WO2021036360A1

WO2021036360A1 - Specific protein analyzer and test method based on same

Info

Publication number: WO2021036360A1
Application number: PCT/CN2020/091079
Authority: WO
Inventors: 张兆伟
Original assignee: 深圳市锦瑞生物科技有限公司
Priority date: 2019-08-29
Filing date: 2020-05-19
Publication date: 2021-03-04
Also published as: CN110632288A

Abstract

A specific protein analyzer, comprising: a main unit (2) and an automatic sampler (3). The automatic sampler (3) comprises a blending assembly (31), an automatic sampling assembly (32), and a refrigeration bin assembly (33). The automatic sampling assembly (32) comprises a test tube rack sampling region (321), a test tube rack feeding region (322), and a test tube rack unloading region (323). The automatic sampling assembly (32) is used for sequentially conveying test tube racks placed in the test tube rack sampling region (321) to the test tube rack feeding region (322) and the test tube rack unloading region (323). Each test tube rack comprises a test tube carrying a sample to be tested. The blending assembly (31) is used for blending the test tubes. The refrigeration bin assembly (33) is provided with two antibody bottle placement positions. By configuring the automatic sampler (3), the automatic sampler (3) conveys the test tube racks placed in the test tube rack sampling region (321) thereof to the test tube rack feeding region (322), thereby implementing automatic sampling.

Description

A specific protein analyzer and test method based on it

Technical field

This application relates to the field of medical devices, and in particular to a specific protein analyzer and a test method based on it.

Background technique

At present, the specific protein analyzer is used as a mainstream instrument for the analysis of specific protein content in human body fluids, and is widely used. The specific protein analyzer uses the principle of scattering and turbidity for detection, by detecting the immune complex particles formed by the reaction between the antigen and the antibody suspended in the buffer. When the laser passes through, the intensity of the scattered light generated by the suspended particles is proportional to the concentration of the antigen. When the reaction reaches the highest peak, the amount of product formed is measured. The peak rate is proportional to the content of the antigen. The peak rate is converted into The concentration of the tested antigen.

CRP and SAA are currently recognized important indicators in the medical field to identify bacterial or viral infections, and the ratio SAA/CRP also has clinical guiding significance. Existing instruments for measuring specific proteins can only inject samples manually, which is inconvenient to operate.

Summary of the invention

In order to solve the above technical problems, embodiments of the present application provide a specific protein analyzer capable of automatic sample injection and a test method based on it.

In order to solve the above technical problems, the embodiments of the present application provide the following technical solutions:

In one aspect, a specific protein analyzer is provided, which includes a host, the host includes a housing, and a reaction module, a sampling component, a liquid addition component, a fluid path system, and a control board installed in the housing. The control board is used to control the reaction module, the sampling component, the liquid addition component, and the liquid path system, the reaction module is used to provide a reaction site for antigen and antibody, and the liquid addition component is used for For adding reagents to the reaction module, the sampling component is used to aspirate samples and/or antibodies, the fluid path system is connected to the fluid addition component and the sampling component, and the fluid path system is used to transport reagents, Sample and/or antibody; and an autosampler, the autosampler includes a mixing assembly, an autosampler assembly, and a refrigerating chamber assembly, the autosampler assembly includes a test tube rack sampling area and a test tube rack feeding area And a test tube rack unloading area, where the automatic sample introduction component is used to sequentially transport the test tube racks placed in the test tube rack sampling area to the test tube rack feeding area and the test tube rack unloading area, the test tube rack including A test tube containing a sample to be tested, the mixing component is used for mixing the test tube, and the refrigerating chamber component is provided with two antibody bottle placement positions.

In some embodiments, the reaction module includes a module base, a laser, a diaphragm, a photoelectric conversion plate, a shielding cover, and a reaction cup group; the laser, the diaphragm, the reaction cup group, the The photoelectric conversion board and the shielding cover are both installed on the module base and arranged in sequence along the optical axis of the laser; the laser is used to emit laser light to the reaction cup group through the aperture; The diaphragm is used to filter the laser light emitted by the laser; the reaction cup group is used to provide a reaction place for antigen and antibody; the photoelectric conversion plate is used to receive scattered light from the reaction cup group and convert it into an electrical signal; The liquid component is used to add reagents in the reaction cup group.

In some embodiments, the reaction module further includes a stirring motor and a magnet, the magnet is fixedly installed on the rotating shaft of the stirring motor; the reaction cup group includes a reaction cup and a heating block; the reaction cup is used to provide a reaction Place, the reaction cup is placed with a magnetic rod stirrer, when the stirring motor drives the magnet to rotate, the magnetic rod stirrer rotates together; the outer surface of the heating block is covered with a heating film for providing reaction The temperature required for the reaction of the place.

In some embodiments, the sampling assembly includes a sampling holder, a puncture support, a feed drive device, a puncture drive device, a sampling needle, and a swab; the puncture support is movably installed on the sampling fixture, and the sampling needle It is movably mounted on the puncture support, and the swab is fixedly mounted on the puncture support; the feed drive device is used to drive the puncture support to move in the horizontal direction; the puncture drive device is used to drive the sampling needle along Move in a vertical direction; the swab is used to clean the sampling needle when the sampling needle moves.

In some embodiments, the liquid addition assembly includes a liquid addition bottom plate, a liquid addition tube holder, a liquid addition tube, and a liquid addition driving device; the liquid addition tube socket is movably installed on the liquid addition bottom plate, and the liquid addition tube It is fixedly installed on the liquid adding pipe seat; the liquid adding driving device is used for driving the liquid adding pipe seat to move along the feeding direction, so that the liquid adding pipe adds reagents to the reaction module.

In some embodiments, the mixing assembly includes a first linear driving device, a second linear driving device, a rotary driving device, and a clamping jaw; the first linear driving device is used to drive the clamping jaw to move in a horizontal direction; The second linear driving device is used for driving the clamping jaw to move in a vertical direction; the rotation driving device is used for driving the clamping jaw to rotate; the clamping jaw is used for clamping the test tube.

In some embodiments, the first linear drive device includes a first screw mechanism and a first screw drive motor, and the first screw drive motor is used to drive the second screw mechanism through the first screw mechanism. The linear drive device moves in a horizontal direction; the second linear drive device includes a second screw mechanism and a second screw drive motor, and the second screw drive motor is used to drive the second screw mechanism The rotary drive device moves in a vertical direction; the rotary drive device includes a pulley mechanism and a pulley drive motor, and the pulley drive motor is used to drive the clamping jaw to rotate through the pulley mechanism.

In some embodiments, the automatic sampling assembly further includes a bottom plate, a sampling assembly, a back plate assembly, a lateral injection assembly, an unloading assembly, and a scanner assembly; the sampling assembly includes a first linear slide, The sampling claw, the first motor, and the first pulley assembly, the first linear slide rail is used to guide the test tube rack to be transported from the test tube rack sampling area to the test tube rack feeding area, The sampling pawl is used to drive the test tube rack to be transported from the test tube rack sampling area to the test tube rack feeding area, and the first motor is used to drive the sample injection dial through the first pulley assembly Claw; the back plate assembly includes a back plate and a counter installed on the back plate, the counter is used to count the test tube position of the test tube rack; the horizontal injection assembly includes a horizontal injection bottom plate and installation A second motor, a second linear guide rail, a second belt wheel assembly, and a transverse pawl assembly on the transverse sample introduction base plate, the second linear guide rail is used to guide the test tube rack from the test tube rack feeding area Transported to the test tube rack unloading area, the lateral sample feeding pawl is used to drive the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, and the second motor is used to pass through the test tube rack unloading area. The second pulley assembly drives the lateral sample feeding pawl; the unloading assembly includes an unloading bracket and a third motor installed on the unloading bracket, a third pulley assembly, a third linear guide, and an unloading push plate. The third linear guide rail is used to guide the test tube rack to be unloaded from the test tube unloading area, the unloading push plate is used to drive the test tube rack to unload from the test tube unloading area, and the third motor is used to pass through the first The three-wheel assembly drives the unloading push plate; the scanner assembly is used to scan the barcode affixed to the test tube; the refrigeration chamber is cooled by the cold end of the Peltier semiconductor, and the hot end of the Peltier semiconductor Installed on the radiator, the radiator dissipates heat through a fan.

In some embodiments, the autosampler is provided with a key switch for controlling the sampling component to aspirate samples and/or antibodies.

On the other hand, there is provided a test method based on the above-mentioned specific protein analyzer, the method comprising: the sampling component diluting the sample in the test tube and spitting the diluted sample and antibody to the Reaction cup group; after a preset time, the reaction module collects data.

In some embodiments, the sampling component dilutes the sample and spits the diluted sample and antibody into the reaction cup group, which specifically includes: the sampling component sucks the sample in the test tube; the sampling component moves To the pre-dilution position and spit out the sample; the sampling component moves to the antibody position to suck the antibody; the sampling component returns to the pre-dilution position to suck the diluted sample; the sampling component moves to the reaction cup group to spit out the sample and antibody; Stir the antigen and antibody in the reaction cup group.

Compared with the prior art, in the specific protein analyzer and the test method based on the specific protein analyzer provided in the embodiments of the present application, by configuring an autosampler, the autosampler transports the test tube rack placed in its test tube rack sampling area to The feeding area of the test tube rack realizes a specific protein analyzer that can automatically inject samples.

Description of the drawings

One or more embodiments are exemplified by the accompanying drawings. These exemplified descriptions do not constitute a limitation on the embodiments. Elements with the same reference numerals in the drawings are denoted as similar elements, unless there are special Affirm that the figures in the attached drawings do not constitute a limitation of proportion.

Figure 1 is a three-dimensional view of a specific protein analyzer provided by one of the embodiments of the application;

Figure 2 is a schematic diagram of the disassembly of the specific protein analyzer shown in Figure 1;

Fig. 3 is a schematic diagram showing the disassembly of the specific protein analyzer shown in Fig. 1 from another angle;

4 is a cross-sectional view of the reaction module of the specific protein analyzer shown in FIG. 1;

Fig. 5 is a three-dimensional view of the sampling component of the specific protein analyzer shown in Fig. 1;

Fig. 6 is a three-dimensional view of the liquid addition component of the specific protein analyzer shown in Fig. 1;

FIG. 7 is a schematic diagram of disassembling the automatic sampling assembly of the specific protein analyzer shown in FIG. 1;

Fig. 8 is a three-dimensional view of the mixing component of the specific protein analyzer shown in Fig. 1;

FIG. 9 is a flowchart of a test method based on the specific protein analyzer shown in FIG. 1 according to another embodiment of the embodiment of the application;

Fig. 10 is a detailed flowchart of step S1 in Fig. 9.

detailed description

In order to facilitate the understanding of the application, the application will be described in more detail below in conjunction with the drawings and specific implementations. It should be noted that when an element is expressed as being "fixed to" another element, it may be directly on the other element, or there may be one or more elements in between. When an element is said to be "connected" to another element, it can be directly connected to the other element, or there may be one or more intervening elements in between. The terms "upper", "lower", "left", "right", "inner", "outer" and similar expressions used in this specification are for illustrative purposes only.

Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by those skilled in the technical field of this application. The terms used in the specification of this application in this specification are only for the purpose of describing specific implementations, and are not used to limit the application. The term "and/or" used in this specification includes any and all combinations of one or more related listed items.

Please refer to FIG. 1, a specific protein analyzer provided by one of the embodiments of this application includes a front housing 1, a host 2 and an autosampler 3. The front side of the front shell 1 is equipped with a display screen 4 for human-computer interaction and display of test results. The host 2 is equipped with a thermal printer 5, and the output port of the thermal printer 5 is set on the top of the host 2 for printing and outputting test results. The autosampler 3 is equipped with a key switch 6 for manual sample injection mode and emergency mode. The autosampler 3 includes a refrigerating chamber assembly, and the refrigerating chamber assembly is provided with two antibody bottle placement positions, so that the specific protein analyzer can not only support a single CRP, SAA project test, but also support CRP, SAA joint inspection. The refrigeration bin assembly is cooled by the cold end of the Peltier semiconductor, and the hot end of the Peltier semiconductor is installed on a radiator, and the radiator is radiated by a fan.

Referring to FIGS. 2 and 3, the host 2 includes a casing and a reaction module 21, a sampling component 22, a liquid addition component 23, a liquid path system, and a control board 24 installed in the casing. The bottom plate 25 of the casing is installed with a first mounting plate and a second mounting plate, the first mounting plate and the second mounting plate are opposite and both are vertical on the bottom plate. The reaction module 21 is installed on the bottom plate 25 to provide a reaction place for antigen and antibody. The sampling component 22 and the liquid addition component 23 are both installed on the first mounting plate. The sampling component 22 is used to suck and spit samples and/or antibodies, and the liquid addition component 23 is used to add reagents to the reaction module 21. The liquid path system is installed between the first mounting plate and the second mounting plate, and is connected to the sampling assembly 22 and the liquid adding assembly 23 for transporting reagents, samples and/or antibodies. The control board card 24 is installed above the first mounting plate and the second mounting plate, and is connected to the autosampler 3, the reaction module 21, the sampling component 22, the liquid addition component 23, and the liquid path system, and is used to control the autosampler 3. The reaction module 21, the sampling component 22, the liquid adding component 23, and the liquid path system work.

Please refer to FIG. 4, the reaction module 21 includes a module base 211, a laser 212, an aperture 213, a photoelectric conversion plate 214, a shielding cover 215, a reaction cup group 216, a stirring motor 217 and a magnet 218. The laser 212, the diaphragm 213, the reaction cup group 216, the photoelectric conversion plate 214, and the shielding cover 215 are all installed on the module base 211, and are arranged in sequence along the optical axis O of the laser 212. The laser 212 is connected to the control board. The laser 212 is used to emit laser light to the reaction cup group 216 through the aperture 213 to provide a light source for testing. The diaphragm 213 is used to filter the laser light emitted by the laser 212 to filter out stray light. The reaction cup group 216 is used to provide a reaction place for the antigen and antibody. The reaction cup group 216 receives the laser light emitted by the laser 212 and transmits the scattered light to the photoelectric conversion plate 214. The photoelectric conversion board 214 is connected to the control board card, and the photoelectric conversion board 214 is used to receive the scattered light transmitted by the reaction cup group 216 and convert the light signal into an electrical signal to realize the principle of scattering and turbidity. The shielding cover 215 provides a reference plane for the photoelectric conversion board 214 and shields external electromagnetic signals. The stirring motor 217 is connected to the control board, the magnet 218 is fixedly installed on the rotating shaft of the stirring motor 217, and the reaction cup group 216 includes a reaction cup and a heating block. The reaction cup is used to provide a reaction place for antigen and antibody. The reaction cup receives the laser light emitted by the laser 212 and transmits the scattered light to the photoelectric conversion plate 214. A magnetic rod stir bar is placed in the reaction cup. When the stirring motor 217 drives the magnet 218 to rotate, The magnetic stir bar rotates together to make the antigen and antibody in the reaction cup fully bind and react. The surface of the heating block is covered with a heating film to provide the temperature conditions required for the antigen-antibody reaction.

Referring to FIG. 5, the sampling assembly 22 includes a sampling fixing seat 221, a puncture support 222, a feeding driving device 223, a puncturing driving device 224, a sampling needle 225 and a swab 226. The puncture bracket 222 is movably installed on the sampling fixing seat 221, the sampling needle 225 is movably installed on the puncture bracket 222 and connected to the fluid path system, and the swab 226 is fixedly installed on the puncture bracket 222. The feed drive device 224 is connected to the control board. The feed drive device 224 is used to drive the puncture support 222 to move in the horizontal direction, so that the sampling needle 225 installed on the puncture support 222 is located directly above the test tube or the reaction cup group. The driving device 224 may be realized by a transmission mode in which a pulley drives a belt. The puncture drive device 224 is connected to the control board. The puncture drive device 224 is used to drive the sampling needle 225 to move in the vertical direction, so that the sampling needle located directly above the test tube or reaction cup group extends into the test tube or reaction cup group. The driving device 224 needs to drive the sampling needle 225 to complete the puncturing action in the vertical direction, so the puncturing driving device 224 should be implemented by a screw drive method. The sampling needle 225 is used to aspirate samples and/or antibodies, and a wire harness and an infusion tube of the fluid system can be arranged between the sampling fixing seat 221 and the puncture support 224 through a drag chain. The swab 226 is used to clean the sampling needle when the sampling needle 225 moves. The aforementioned key switch can also control the sampling component 22 to aspirate samples and/or antibodies.

Please refer to FIG. 6, the liquid adding component 23 includes a liquid adding bottom plate 231, a liquid adding pipe seat 232, a liquid adding pipe 233 and a liquid adding driving device 234. The liquid adding pipe seat 232 is movably installed on the liquid adding bottom plate 231, and the liquid adding pipe 233 is fixedly installed on the liquid adding pipe seat 232, and is connected with the liquid circuit system. The liquid addition driving device 234 is connected to the control board. The liquid addition driving device 234 is used to drive the liquid addition tube holder 232 to move in the horizontal direction, so that the liquid addition tube 233 is located above the reaction cup group, so that the liquid addition tube 233 can be aligned with each other. Reagents are added to the reaction cup group. Since the load of the liquid addition driving device 234 is relatively small, the liquid addition driving device 234 can be realized by a transmission mode in which a pulley drives a belt.

Referring to FIGS. 7 and 8, the autosampler 3 further includes a mixing component 31, an autosampler component 32 and the aforementioned refrigerating chamber component 33. The automatic sampling assembly 32 includes a test tube rack sampling area 321, a test tube rack feeding area 322 and a test tube rack unloading area 323. The automatic sample introduction component 32 is used to transfer the test tube rack placed in the test tube rack sampling area 321 to the test tube rack feeding area 322 and the test tube rack unloading area 323 in sequence. The test tube rack includes test tubes containing samples to be tested. The mixing component 31 is used for mixing the test tube. The automatic sample injection component 32 also includes a bottom plate, a sample injection component, a back plate component, a lateral injection component, an unloading component, and a scanner component. The sampling assembly includes a first linear slide rail, a sampling claw, a first motor, and a first pulley assembly. The first slide rail is used to guide the test tube rack to be transported from the test tube rack sampling area to the test tube rack feeding area, and the sampling claw is used to drive the test tube rack from the test tube rack sampling area to the test tube rack feeding area. The first motor is used The sample feeding pawl is driven by the first belt wheel assembly. The backrest assembly includes a backrest and a counter installed on the backrest. The counter is connected to the control board. The counter is used to count the test tube positions of the test tube rack. Each time the test tube rack feeds a test tube position, the counter counts the test tube position. Add one bit to calculate the feed position of the test tube rack. The horizontal injection assembly includes a horizontal injection bottom plate, a second motor installed on the horizontal injection bottom plate, a second linear guide rail, a second pulley assembly, and a horizontal pawl assembly. The second linear guide rail is used to guide the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, and the transverse sampling pawl is used to drive the test tube rack to be transported from the test tube rack feeding area to the test tube rack unloading area, and the second motor is connected to control The board card, the second motor is used to drive the transverse sample feeding pawl through the second pulley assembly. The unloading assembly includes an unloading bracket, a third motor installed on the unloading bracket, a third pulley assembly, a third linear guide, and an unloading push plate. The third linear guide rail is used to guide the test tube rack to unload from the test tube rack unloading area, the unloading push plate is used to drive the test tube rack to unload from the test tube rack unloading area, the third motor is connected to the control board, and the third motor is used to pass the third pulley assembly Drive to unload the push plate. The scanner component is connected to the control board card, and the scanner component is used to scan the bar code attached to the test tube to identify the test tube.

The mixing assembly 31 includes a first linear driving device 311, a second linear driving device 312, a rotating driving device 313 and a clamping jaw 314. The first linear driving device 311 is connected to the control board. The first linear driving device 311 is used to drive the clamping jaw 314 to move in a horizontal direction, so that the clamping jaw 314 is located directly above the test tube. The first linear drive 311 device includes a first screw mechanism and a first screw drive motor. The first screw drive motor is connected to the control board. The first screw drive motor is used to drive the second linear drive through the first screw mechanism. The driving device 312 moves in the horizontal direction. The second linear driving device 312 is connected to the control board. The second linear driving device 312 is used to drive the clamping jaws 314 to move in the vertical direction, so that the clamping jaws 314 are close to the test tube rack, so that the clamping jaws 314 can grip the test tube. The second linear drive device 312 includes a second screw mechanism and a second screw drive motor. The second screw drive motor is connected to the control board. The second screw drive motor is used to drive the rotary drive device 313 through the second screw mechanism. Move in the vertical direction. The rotation driving device 313 is connected to the control board, and the rotation driving device 313 is used to drive the clamping jaws 314 to rotate so as to mix the test tubes clamped by the clamping jaws 314 evenly. The rotation driving device 313 includes a pulley mechanism and a pulley drive motor. The pulley drive motor is connected to the control board. The pulley drive motor is used to drive the clamping jaw to rotate through the pulley mechanism. The clamping jaw 314 is connected to the control board card, and the clamping jaw 314 is used to clamp the test tube.

When the above-mentioned specific protein analyzer performs automatic sample injection operation, it is as follows:

The test tube rack includes test tubes containing samples to be tested. The test tube rack is clamped in the test tube rack sampling area, and the test tube rack is transported from the test tube rack sampling area to the test tube rack feeding area through the sample injection component. When the test tube position counted by the counter indicates that the test tube rack has entered the test tube rack feeding area, the scanner assembly scans the barcode affixed on the test tube to identify the test tube, and the mixing component clamps the test tube and puts it back into the test tube rack after mixing. , And then, the sampling component sucks samples and/or antibodies to the test tube. After the final sampling component has absorbed the samples and antibodies, the horizontal injection component transports the test tube rack to the test tube rack unloading area, and the unloading component will be located in the test tube unloaded from the test tube rack Shelf unloading.

The manual injection process of the above-mentioned specific protein analyzer is as follows:

Place the test tube under the sampling needle of the sampling assembly, ensure that the inlet of the sampling needle is below the sample liquid level, trigger the button switch 6, the sampling assembly sucks up the sample and/or antibody, and finally the sampling assembly sucks up the sample and antibody.

After the above-mentioned specific protein analyzer completes automatic sample injection or manual sample injection, the sampling component spits the drawn sample and antibody into the reaction cup, and the liquid addition component is the reaction cup for adding reagents, and the magnetic rod in the reaction cup is driven by the stirring motor After the stir bar is stirred, the laser emits laser light to the reaction cup, the photoelectric conversion board receives the scattered light transmitted by the reaction cup, and generates an electrical signal containing the test result, transmits it to the control board, and displays it on the display screen. At this time, the test process is completed. .

Referring to FIG. 9, another embodiment of the present application provides a test method based on the specific protein analyzer provided in the foregoing embodiment, and the test method includes:

Step S1: The sampling component spits the diluted sample and antibody into the reaction cup group;

Step S2: After a preset time, the reaction module collects data. This data is the test result, including CRP value, SAA value and SAA/CRP ratio.

Referring to FIG. 10, in step S1, the sampling component spits the diluted sample and antibody into the reaction cup group, which specifically includes:

S11: The sampling component sucks a sample in the test tube;

S12: The sampling component moves to the pre-dilution position and spit out the sample;

S13: The sampling component is moved to the antibody position to absorb the antibody;

S14: The sampling component returns to the pre-dilution position to suck the diluted sample;

S15: The sampling component is moved to the reaction cup group to vomit samples and antibodies;

S16: Stirring the antigen and antibody in the reaction cup group.

Compared with the prior art, in the specific protein analyzer provided in the embodiments of the present application and the test method based on the same, by configuring an autosampler, the autosampler transports the test tube rack placed in the test tube rack sampling area of the test tube rack to the test tube The rack feeding area realizes a specific protein analyzer that can automatically inject samples.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, not to limit them; under the idea of this application, the above embodiments or the technical features in different embodiments can also be combined. The steps can be implemented in any order, and there are many other changes in different aspects of the application as described above. For the sake of brevity, they are not provided in the details; although the application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art It should be understood that it is still possible to modify the technical solutions recorded in the foregoing embodiments, or equivalently replace some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technology of the embodiments of this application The scope of the program.

Claims

A specific protein analyzer, which is characterized in that it comprises:

A host, the host includes a housing and a reaction module, a sampling component, a liquid addition component, a fluid path system, and a control board installed in the housing, and the control board is used to control the reaction module, The sampling component, the liquid addition component, and the liquid path system, the reaction module is used to provide a reaction place for antigen and antibody, the liquid addition component is used to add reagents to the reaction module, the sampling component For aspirating samples and/or antibodies, the liquid path system is connected to the liquid adding component and the sampling component, and the liquid path system is used to transport reagents, samples and/or antibodies; and

An autosampler, the autosampler includes a mixing component, an autosampler component, and a refrigeration chamber component, and the autosampler includes a test tube rack sampling area, a test tube rack feeding area, and a test tube rack unloading area. The automatic sample introduction component is used to sequentially transport the test tube racks placed in the test tube rack sampling area to the test tube rack feeding area and the test tube rack unloading area, and the test tube rack includes test tubes containing samples to be tested The mixing component is used for mixing the test tube, and the refrigerating chamber component is provided with two antibody bottle placement positions.
The specific protein analyzer of claim 1, wherein the reaction module comprises a module base, a laser, an aperture, a photoelectric conversion plate, a shielding cover, and a reaction cup group;

The laser, the diaphragm, the reaction cup group, the photoelectric conversion board and the shielding cover are all installed on the module base and arranged in sequence along the optical axis of the laser;

The laser is used to emit laser light to the reaction cup group through the aperture;

The diaphragm is used to filter the laser light emitted by the laser;

The reaction cup set is used to provide a reaction place for antigen and antibody;

The photoelectric conversion board is used to receive the scattered light of the reaction cup group and convert it into an electrical signal;

The liquid adding component is used for adding reagents in the reaction cup group.
The specific protein analyzer of claim 2, wherein the reaction module further comprises a stirring motor and a magnet, and the magnet is fixedly installed on the rotating shaft of the stirring motor;

The reaction cup group includes a reaction cup and a heating block;

The reaction cup is used to provide a reaction place, and a magnetic rod stirrer is placed in the reaction cup, and when the stirring motor drives the magnet to rotate, the magnetic rod stirrer rotates together;

The outer surface of the heating block is covered with a heating film to provide the temperature required for the reaction in the reaction site.
The specific protein analyzer according to claim 1, wherein the sampling assembly comprises a sampling holder, a puncture support, a feed drive device, a puncture drive device, a sampling needle and a swab;

The puncture support is movably mounted on the sampling fixing seat, the sampling needle is movably mounted on the puncture support, and the swab is fixedly mounted on the puncture support;

The feed drive device is used to drive the puncture support to move in a horizontal direction;

The puncture driving device is used to drive the sampling needle to move in a vertical direction;

The swab is used to clean the sampling needle when the sampling needle moves.
The specific protein analyzer according to claim 1, wherein the liquid addition component comprises a liquid addition bottom plate, a liquid addition tube seat, a liquid addition tube, and a liquid addition driving device;

The liquid adding pipe seat is movably installed on the liquid adding bottom plate, and the liquid adding pipe is fixedly installed on the liquid adding pipe seat;

The liquid addition driving device is used to drive the liquid addition tube holder to move along the feeding direction, so that the liquid addition tube adds reagents to the reaction module.
The specific protein analyzer of claim 1, wherein the mixing assembly comprises a first linear drive device, a second linear drive device, a rotary drive device and a clamping jaw;

The first linear driving device is used to drive the clamping jaw to move in a horizontal direction;

The second linear driving device is used to drive the clamping jaw to move in a vertical direction;

The rotation driving device is used to drive the clamping jaws to rotate;

The clamping jaw is used to clamp the test tube.
The specific protein analyzer according to claim 6, wherein the first linear drive device comprises a first screw mechanism and a first screw drive motor, and the first screw drive motor is used to pass the The first screw mechanism drives the second linear drive device to move in a horizontal direction;

The second linear drive device includes a second screw mechanism and a second screw drive motor, and the second screw drive motor is used to drive the rotary drive device to move in a vertical direction through the second screw mechanism ；

The rotation driving device includes a pulley mechanism and a pulley drive motor, and the pulley drive motor is used to drive the clamping jaw to rotate through the pulley mechanism.
The specific protein analyzer according to claim 1, wherein the automatic sample injection component further comprises a bottom plate, a sample injection component, a back plate component, a lateral sample injection component, an unloading component, and a scanner component;

The sampling assembly includes a first linear slide rail, a sampling claw, a first motor, and a first pulley assembly, and the first linear slide rail is used to guide the test tube rack to inject samples from the test tube rack Transport area to the test tube rack feeding area, the sample injection pawl is used to drive the test tube rack to be transported from the test tube rack sampling area to the test tube rack feeding area, and the first motor is used to pass The first belt wheel assembly drives the sample feeding pawl;

The backrest board assembly includes a backrest board and a counter installed on the backrest board, and the counter is used to count the test tube positions of the test tube rack;

The transverse sampling assembly includes a transverse sampling base plate, a second motor, a second linear guide rail, a second pulley assembly, and a transverse pawl assembly mounted on the transverse sampling base plate, and the second linear guide rail is used for The test tube rack is guided to be transported from the test tube rack feeding area to the test tube rack unloading area, and the lateral sample feeding pawl is used to drive the test tube rack to be transported from the test tube rack feeding area to the test tube rack In the unloading area, the second motor is used to drive the lateral sample feeding pawl through the second pulley assembly;

The unloading assembly includes an unloading bracket, a third motor, a third pulley assembly, a third linear guide, and an unloading push plate installed on the unloading bracket, and the third linear guide is used to guide the test tube rack from the The test tube unloading area is unloaded, the unloading push plate is used to drive the test tube rack to unload from the test tube unloading area, and the third motor is used to drive the unloading push plate through the third pulley assembly;

The scanner assembly is used to scan the barcode affixed on the test tube;

The refrigerating chamber body is cooled by the cold end of the Peltier semiconductor, the hot end of the Peltier semiconductor is mounted on a radiator, and the radiator is used for heat dissipation by a fan.
The specific protein analyzer according to any one of claims 1 to 8, wherein the autosampler is provided with a key switch for controlling the sampling component to suck and discharge samples and/or antibodies.
A test method based on the specific protein analyzer according to any one of claims 1 to 9, wherein the test method comprises:

The sampling component spits the diluted sample and antibody to the reaction cup group;

After a preset time, the reaction module collects data.
The test method according to claim 10, wherein the sampling component spitting the diluted sample and antibody into the reaction cup group specifically comprises:

The sampling component sucks a sample in the test tube;

The sampling component moves to the pre-dilution position and spit out the sample;

The sampling component is moved to the antibody position to absorb the antibody;

The sampling component returns to the pre-dilution position to suck the diluted sample;

Moving the sampling component to the reaction cup group to vomit samples and antibodies;

Stir the antigen and antibody in the reaction cup group.