WO2021019624A1 - 細胞回収装置および細胞回収方法 - Google Patents

細胞回収装置および細胞回収方法 Download PDF

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Publication number
WO2021019624A1
WO2021019624A1 PCT/JP2019/029514 JP2019029514W WO2021019624A1 WO 2021019624 A1 WO2021019624 A1 WO 2021019624A1 JP 2019029514 W JP2019029514 W JP 2019029514W WO 2021019624 A1 WO2021019624 A1 WO 2021019624A1
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WIPO (PCT)
Prior art keywords
flow path
pump
valve
pipette tip
mode
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2019/029514
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English (en)
French (fr)
Japanese (ja)
Inventor
大和 前田
明莉 武田
善丈 山本
野田　佳孝
隆志 井上
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Shimadzu Corp
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Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to PCT/JP2019/029514 priority Critical patent/WO2021019624A1/ja
Priority to CN201980098325.9A priority patent/CN114096654B/zh
Priority to JP2021536468A priority patent/JP7231038B2/ja
Priority to US17/628,528 priority patent/US12559708B2/en
Publication of WO2021019624A1 publication Critical patent/WO2021019624A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof

Definitions

  • the present invention relates to a cell recovery device and a method for recovering cells using a cell recovery device.
  • Patent Document 1 a cell aspiration system for assisting the work of picking cells has been proposed in Patent Document 1.
  • the system proposed in Patent Document 1 includes a transport unit that three-dimensionally moves a suction unit having a pipette tip attached to the tip thereof, and a detection unit that optically detects the tip of the pipette tip.
  • the tip of the pipette tip is detected by the detection unit, and the tip position of the pipette tip is moved to the position of a specific cell by the transport unit.
  • Japanese Unexamined Patent Publication No. 2016-112012 Japanese Unexamined Patent Publication No. 2016-112012
  • the cell suction system proposed in Patent Document 1 can assist the operator's work of picking cells.
  • As a method for collecting cells in the cell culture vessel as described above, in addition to the method of picking specific cells, unnecessary substances in the cell culture vessel are removed, and the specific cells remain in the cell culture vessel. There is a way. The method of collecting the cells in these cell culture vessels is still a burdensome task for the operator, and improvement is desired.
  • An object of the present invention is to reduce the burden on the operator for collecting cells in a cell culture vessel.
  • the cell recovery device includes a pipette tip for sucking a substance in a cell culture vessel, a first valve connected to the pipette tip via a first flow path, a first valve and a second flow path.
  • a first pump connected via a third valve and a second pump connected via a third flow path are provided.
  • the first valve is switched so as to connect the first flow path and the second flow path and block the third flow path, the first pump is driven, and the inside of the cell culture vessel sucked from the pipette tip. Is discharged from the drain of the first pump via the first flow path and the second flow path, and in the picking mode, the first flow path and the third flow path are connected and the second flow path is cut off.
  • the first valve is switched so as to drive the second pump, and the cells in the cell culture vessel are sucked by the pipette tip.
  • FIG. 1 is an overall view of the cell recovery device according to the present embodiment.
  • FIG. 2 is a diagram showing a cell recovery device operating in the remove mode.
  • FIG. 3 is a diagram showing a cell recovery device operating in the washing mode.
  • FIG. 4 is a diagram showing a cell recovery device operating in the picking mode.
  • FIG. 5 is a flowchart showing the operation of the remove mode.
  • FIG. 6 is a flowchart showing the operation of the cleaning mode.
  • FIG. 7 is a flowchart showing the operation of the picking mode.
  • FIG. 1 is an overall view of the cell recovery device 10 according to the present embodiment.
  • the cell recovery device 10 of the present embodiment includes three modes, a remove mode, a washing mode, and a picking mode.
  • the remove mode is a mode in which cells are collected by sucking and removing unnecessary substances in the cell culture vessel 21 and leaving specific cells in the cell culture vessel 21.
  • the washing mode is a mode for washing the flow path included in the cell recovery device 10.
  • the picking mode is a mode in which cells are collected by sucking specific cells in the cell culture vessel 21.
  • the cell recovery device 10 includes a pipette tip 1, a drive unit 2, an optical unit 3, an imaging unit 4, a control unit 5, a display unit 6, an operation unit 7, and a flow path 8.
  • the pipette tip 1 sucks unnecessary substances in the cell culture vessel 21 in the remove mode, and sucks specific cells in the cell culture vessel 21 in the picking mode.
  • the pipette tip 1 sucks the cleaning liquid in the cleaning mode.
  • the first flow path R1 is connected to the pipette tip 1.
  • the drive unit 2 moves the pipette tip 1 in the three-dimensional direction.
  • the drive unit 2 includes, for example, a rotation drive unit in a horizontal plane, a rotation drive unit in a circular surface, a movement drive unit in the vertical direction, and the like.
  • the control unit 5 controls the cell recovery device 10.
  • the drive unit 2 moves the pipette tip 1 under the control of the control unit 5.
  • the optical unit 3 optically detects the tip of the pipette tip 1.
  • the optical unit 3 includes a microscope with a field of view above the installation table 20.
  • the image of the tip of the pipette tip 1 detected by the optical unit 3 is imaged by the imaging unit 4.
  • the image pickup unit 4 is, for example, an image pickup device such as a CCD (Charge Coupled Device).
  • the image of the tip of the pipette tip 1 captured by the imaging unit 4 is given to the control unit 5.
  • the control unit 5 acquires an image of the tip of the pipette tip 1 and displays the image of the tip of the pipette tip 1 on the display unit 6.
  • the display unit 6 is, for example, a liquid crystal display.
  • the image displayed on the display unit 6 includes an image of the tip of the pipette tip 1 and an image of the culture solution CS containing the cells contained in the cell culture container 21. The operator can grasp the positional relationship between the pipette tip 1 and the cell by referring to the image.
  • the operation unit 7 includes an operation member for moving the pipette tip 1 in the three-dimensional direction and an operation member for causing the pipette tip 1 to perform suction and discharge operations.
  • the operator operates the operation unit 7 to move the tip of the pipette tip 1 while referring to the image of the culture solution CS containing the tip of the pipette tip 1 and the cells displayed on the display unit 6. Further, the operator executes a suction or discharge operation with respect to the pipette tip 1 while referring to the image of the culture solution CS containing the tip of the pipette tip 1 and the cells displayed on the display unit 6.
  • the flow path 8 includes a first valve V1, a second valve V2, a first pump P1 and a second pump P2.
  • the pipette tip 1 and the first valve V1 are connected by the first flow path R1.
  • the first valve V1 and the second valve V2 are connected by the fourth flow path R4.
  • the second valve V2 and the first pump P1 are connected by a fifth flow path R5.
  • the first valve V1 and the second pump P2 are connected by a third flow path R3.
  • the second pump P2 and the second valve V2 are connected by the sixth flow path R6.
  • the second flow path R2 is formed by the fourth flow path R4 and the fifth flow path R5.
  • the first flow path R1 is connected to the suction port of the pipette tip 1, and in the remove mode, the culture solution CS containing unnecessary substances sucked by the pipette tip 1 flows into the first flow path R1. Then, in the remove mode, the culture solution CS containing unnecessary substances flows through the first flow path R1 and the second flow path R2 (fourth flow path R4 and fifth flow path R5). Further, in the cleaning mode, the cleaning liquid sucked by the pipette tip 1 flows into the first flow path R1. Then, in the cleaning mode, the cleaning liquid flows through the first flow path R1, the third flow path R3, the sixth flow path R6, and the fifth flow path R5. Further, in the picking mode, the first flow path R1, the third flow path R3, and the sixth flow path R6 are filled with the culture solution in advance.
  • the first valve V1 is a valve having three ports connected to the first flow path R1, the fourth flow path R4 (second flow path R2), and the third flow path R3, respectively.
  • the first valve V1 is a first switching state that connects the first flow path R1 and the fourth flow path R4 (second flow path R2) and shuts off the third flow path R3, and the first flow path R1 and It is possible to switch between the second switching state in which the third flow path R3 is connected and the fourth flow path R4 (second flow path R2) is blocked.
  • the first valve V1 switches between the first switching state and the second switching state under the control of the control unit 5.
  • the second valve V2 is a valve having three ports connected to the fourth flow path R4, the fifth flow path R5, and the sixth flow path R6, respectively.
  • the second valve V2 connects the fourth flow path R4 and the fifth flow path R5, and also connects the first switching state that shuts off the sixth flow path R6 and the sixth flow path R6 and the fifth flow path R5. At the same time, it is possible to switch between the second switching state in which the fourth flow path R4 is blocked.
  • the second valve V2 switches between the first switching state and the second switching state under the control of the control unit 5.
  • the first pump P1 is a motor driven in the remove mode and the cleaning mode.
  • the first pump P1 is driven by the control of the control unit 5.
  • the first pump P1 is a motor having a larger capacity and stronger power than the second pump P2.
  • a diaphragm pump is used as the first pump P1.
  • the first pump P1 sucks the culture solution CS containing unnecessary substances through the first flow path R1 and the second flow path R2 (fourth flow path R4 and fifth flow path R5).
  • the first pump P1 sucks the cleaning liquid through the first flow path R1, the third flow path R3, the sixth flow path R6, and the fifth flow path R5.
  • the first pump P1 discharges the culture solution CS or the cleaning solution containing the sucked unnecessary substances from the drain D1.
  • the second pump P2 is a motor driven in the picking mode.
  • the second pump P2 is driven by the control of the control unit 5.
  • the second pump P2 is a motor having a smaller capacity than the first pump P1 and having high accuracy in controlling the suction and discharge amounts.
  • a plunger pump is used as the second pump P2.
  • the second pump P2 sucks the culture solution from the third flow path R3 during the suction operation in the picking mode.
  • the second pump P2 discharges the culture solution sucked into the cylinder to the third flow path R3 during the discharge operation in the picking mode.
  • FIG. 2 is a diagram showing a cell recovery device 10 operating in the remove mode.
  • FIG. 5 is a flowchart showing the operation of the remove mode.
  • the remove mode is a mode in which cells are collected by sucking and removing unnecessary substances in the cell culture vessel 21 and leaving specific cells in the cell culture vessel 21.
  • a cell culture container 21 is placed on the upper part of the setting table 20.
  • the cell culture container 21 contains a culture solution CS containing cells to be collected.
  • step S11 shown in FIG. 5 the control unit 5 switches the first valve V1 to connect the first flow path R1 and the second flow path R2 (fourth flow path R4), and shuts off the third flow path R3. To do. Further, in step S12, the control unit 5 switches the second valve V2 to connect the fourth flow path R4 and the fifth flow path R5, and shuts off the sixth flow path R6. Next, in step S13, the control unit 5 drives the first pump P1 to suck the culture solution CS containing unnecessary substances in the cell culture container 21 with the pipette tip 1.
  • the culture solution CS containing unnecessary substances sucked by the pipette tip 1 passes through the first flow path R1 and the second flow path R2 (fourth flow path R4 and fifth flow path R5) to drain D1 of the first pump P1. Is discharged from.
  • the flow path painted in black is the flow path through which the culture solution CS containing unnecessary substances sucked by the pipette tip 1 flows.
  • the culture solution CS containing the sucked unnecessary substances is discharged from the drain D1.
  • the cells to be collected remain in the cell culture vessel 21.
  • FIG. 3 is a diagram showing a cell recovery device 10 operating in the washing mode.
  • FIG. 6 is a flowchart showing the operation of the cleaning mode.
  • the cleaning mode is a mode that mainly cleans the flow path including the first flow path R1.
  • the first flow path R1 is a flow path shared in the remove mode and the picking mode.
  • the washing mode is executed, for example, after performing the cell collection operation in the remove mode and before performing the cell collection operation in the picking mode.
  • the pipette tip 1 moves and is arranged in the washing liquid tank 23.
  • the pipette tip 1 sucks the cleaning liquid by performing a suction operation.
  • the cleaning liquid sucked by the pipette tip 1 flows into the first flow path R1.
  • step S21 shown in FIG. 6, the control unit 5 switches the first valve V1 to connect the first flow path R1 and the third flow path R3, and shuts off the second flow path R2 (fourth flow path R4). To do. Further, in step S22, the control unit 5 switches the second valve V2 to connect the sixth flow path R6 and the fifth flow path R5, and shuts off the fourth flow path R4. Next, in step S23, the control unit 5 drives the first pump P1 and draws the cleaning liquid sucked from the pipette tip 1 into the first flow path R1, the third flow path R3, the sixth flow path R6, and the fifth flow path. It is discharged from the drain D1 of the first pump P1 via the path R5.
  • the flow path painted in black is the flow path through which the cleaning liquid sucked by the pipette tip 1 flows.
  • the suctioned cleaning liquid is discharged from the drain D1 by the driving force of the first pump P1.
  • the plunger of the second pump P2 In the cleaning mode, it is preferable to move the plunger of the second pump P2 as much as possible in the discharge direction. As a result, the cleaning liquid flowing through the first flow path R1 and the third flow path R3 due to the suction force of the first pump P1 flows into the sixth flow path R6 without entering the cylinder of the second pump P2. As a result, unnecessary substances mixed with the cleaning liquid and flowing into the cylinder of the second pump P2 are prevented from entering the cylinder.
  • FIG. 4 is a diagram showing a cell recovery device 10 operating in the picking mode.
  • FIG. 7 is a flowchart showing the operation of the picking mode.
  • the picking mode is a mode in which cells are collected by sucking specific cells in the cell culture vessel 21.
  • a cell culture container 21 is placed on the upper part of the setting table 20.
  • the cell culture container 21 contains a culture solution CS containing cells to be collected.
  • the picking mode is prepared by filling the first flow path R1, the third flow path R3, and the sixth flow path R6 with the culture solution.
  • the pipette tip 1 is placed in a container containing a separately prepared culture solution.
  • the first valve V1 and the second valve V2 are switched, and the first pump P1 is driven after shutting off the fourth flow path R4 as in the cleaning mode.
  • the culture solution sucked by the pipette tip 1 flows through the first flow path R1, the third flow path R3, the sixth flow path R6, and the fifth flow path R5.
  • the first flow path R1, the third flow path R3, and the sixth flow path R6 are filled with the culture solution.
  • step S31 shown in FIG. 7 the control unit 5 switches the first valve V1 to connect the first flow path R1 and the third flow path R3, and shuts off the second flow path R2 (fourth flow path R4). To do. Further, in step S32, the control unit 5 switches the second valve V2 to connect the fourth flow path R4 and the fifth flow path R5, and shuts off the sixth flow path R6. Next, in step S33, the control unit 5 drives the second pump P2 to cause the second pump P2 to perform the suction operation. As a result, the cells in the cell culture vessel 21 are sucked by the pipette tip 1. The cells to be collected sucked by the pipette tip 1 are held at the tip of the pipette tip 1.
  • a small amount of the culture solution in the third flow path R3 is held in the cylinder of the second pump P2 by the suction operation by the pipette tip 1.
  • a suction force is applied to both the third flow path R3 and the sixth flow path R6.
  • the second valve V2 is switched to shut off the sixth flow path R6, the culture solution in the third flow path R3 is sucked into the second pump P2 by the suction operation of the second pump P2. ..
  • the control unit 5 controls the second pump P2 and causes the second pump P2 to perform the discharge operation.
  • the cells held at the tip of the pipette tip 1 are delivered to the collection container 22.
  • the second pump P2 performs the discharge operation, pressure is applied to both the third flow path R3 and the sixth flow path R6.
  • the second valve V2 is switched to shut off the sixth flow path R6, most of the culture solution discharged from the second pump P2 due to the discharge operation of the second pump P2 is in the third flow path. It is pushed out to R3.
  • pressure is applied to the first flow path R1 via the third flow path R3 by the discharge operation of the second pump P2, and the cells held at the tip of the pipette tip 1 are discharged.
  • the flow path painted in black is the flow path filled with the culture solution in the picking mode.
  • the suction operation of the second pump P2 the sucked cells are held at the tip of the pipette tip 1.
  • the cells held in the pipette tip 1 are delivered to the collection container 22 by the discharge operation of the second pump P2.
  • the cell recovery device of the present embodiment can operate both the remove mode and the picking mode by switching between the first valve V1 and the second valve V2. This makes it possible to reduce the workload of the operator who performs the cell collection work. Further, the cell recovery device of the present embodiment can operate the washing mode by switching between the first valve V1 and the second valve V2. Thereby, for example, when the cells are collected in the picking mode, contamination due to unnecessary substances remaining in the remove mode can be prevented.
  • a diaphragm pump is used as the first pump P1 and a plunger pump is used as the second pump P2.
  • the pumps used as the first pump P1 and the second pump P2 are not particularly limited, and other pumps may be used.
  • the first pump P1 since it is used for removing unnecessary substances in the remove mode and sucking the cleaning liquid in the cleaning mode, it is preferable to use a pump having a relatively large capacity and strong power.
  • the second pump P2 since it is necessary to perform a suction operation on a cell-by-cell basis or an intracellular tissue-by-cell unit in the picking mode, it is preferable to use a pump having high accuracy in suction amount and discharge amount.
  • the case where the cell recovery device 10 has three modes of a remove mode, a wash mode, and a picking mode has been described as an example, but the cell recovery device 10 does not have the wash mode and has the remove mode and the picking mode. May be good.
  • the flow path 8 may be cleaned by another method.
  • the second valve V2 and the sixth flow path R6 can be omitted. In that case, the first valve V1 and the first pump P1 may be directly connected.
  • the cell recovery device is A pipette tip that sucks substances in the cell culture vessel, A first valve connected to the pipette tip via a first flow path, A first pump connected to the first valve via a second flow path, A second pump connected to the first valve via a third flow path, With In the remove mode, the first valve is switched so as to connect the first flow path and the second flow path and shut off the third flow path, and the first pump is driven from the pipette tip. Unnecessary substances in the aspirated cell culture vessel are discharged from the drain of the first pump via the first flow path and the second flow path. In the picking mode, the first valve is switched so as to connect the first flow path and the third flow path and block the second flow path, and the second pump is driven to drive the cell culture vessel. The cells inside are aspirated by the pipette tip.
  • cells can be recovered using either the remove mode or the picking mode by switching the first valve. As a result, the burden on the operator for collecting the cells in the cell culture container is reduced.
  • the second flow path includes a fourth flow path and a fifth flow path.
  • the cell recovery device is A second valve connected to the first valve via the fourth flow path, connected to the first pump via the fifth flow path, and connected to the second pump via the sixth flow path.
  • the first valve is switched so as to connect the first flow path and the third flow path and shut off the fourth flow path, and the sixth flow path and the fifth flow path.
  • the second valve is switched so as to cut off the fourth flow path, and the first pump is driven to collect the cleaning liquid sucked from the pipette tip into the first flow path and the third flow path.
  • the drain of the first pump may be discharged through the sixth flow path and the fifth flow path.
  • the flow path used for cell recovery can be washed by switching between the first valve and the second valve.
  • the flow path of the cell collection device can be washed, including the first flow path shared in the remove mode and the picking mode.
  • the culture solution may be filled in advance in the first flow path, the third flow path, and the sixth flow path.
  • the suction amount of the second pump can be adjusted accurately.
  • the cell recovery method is A pipette tip that sucks substances in the cell culture vessel, A first valve connected to the pipette tip via a first flow path, A first pump connected to the first valve via a second flow path, A second pump connected to the first valve via a third flow path, It is a cell recovery method performed in a cell recovery device provided with In remove mode, A step of connecting the first flow path and the second flow path and switching the first valve so as to shut off the third flow path. A step of driving the first pump to discharge unnecessary substances in the cell culture vessel sucked from the pipette tip from the drain of the first pump via the first flow path and the second flow path.
  • the second flow path includes a fourth flow path and a fifth flow path.
  • the cell recovery device is A second valve connected to the first valve via the fourth flow path, connected to the first pump via the fifth flow path, and connected to the second pump via the sixth flow path.
  • With 2 valves In wash mode A step of connecting the first flow path and the third flow path and switching the first valve so as to shut off the fourth flow path.
  • the first pump is driven, and the cleaning liquid sucked from the pipette tip is drained from the first pump via the first flow path, the third flow path, the sixth flow path, and the fifth flow path.
  • the process of discharging from May include.

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PCT/JP2019/029514 2019-07-26 2019-07-26 細胞回収装置および細胞回収方法 Ceased WO2021019624A1 (ja)

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Application Number Priority Date Filing Date Title
PCT/JP2019/029514 WO2021019624A1 (ja) 2019-07-26 2019-07-26 細胞回収装置および細胞回収方法
CN201980098325.9A CN114096654B (zh) 2019-07-26 2019-07-26 细胞回收装置及细胞回收方法
JP2021536468A JP7231038B2 (ja) 2019-07-26 2019-07-26 細胞回収装置および細胞回収方法
US17/628,528 US12559708B2 (en) 2019-07-26 2019-07-26 Cell collecting device and cell collecting method

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PCT/JP2019/029514 WO2021019624A1 (ja) 2019-07-26 2019-07-26 細胞回収装置および細胞回収方法

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