WO2021017317A1 - Method for inhibiting photodegradation of ergothioneine, and application therefor - Google Patents

Abstract

A method for inhibiting the photodegradation of ergothioneine, and an application therefor, comprising the following steps: adding hyaluronate to a solution containing ergothioneine; the content of ergothioneine is 0.0005-0.01 wt%, and the content of hyaluronate is more than 0.1 wt%. Combining hyaluronate and ergothioneine can effectively inhibit the photodegradation of ergothioneine, and the composition can be used in food and healthcare products or cosmetics, having a good moisture-retention effect and stable efficacy.

Description

Method for inhibiting photodegradation of ergothione and its application Technical field

The invention belongs to the field of biochemical industry, and relates to a method for inhibiting the photodegradation of ergothioine, in particular to a composition containing ergothioine and its application.

Background technique

Ergothioneine (ergothioneine) is a rare natural amino acid discovered in a fungus Claviceps purpurea in 1909. Existing studies have found that ergothioneine exists in many animals and plants, and has important physiological activities in the body. Studies have shown that it can eliminate free radicals, anti-inflammatory, maintain DNA biosynthesis, normal cell growth, and cellular immunity. Kind of physiological function.

There are three preparation methods of ergothioine: chemical synthesis, extraction and microbial fermentation. Chemical synthesis of levothionine is very difficult. The content of thioneine extracted from natural biological raw materials such as edible fungi fruiting bodies, grains, and animal tissues is still very low, and there are problems such as many impurities in the raw materials, drug residues, and high extraction costs. Although synthesizing ergothioine by microbial fermentation can effectively increase the yield of ergothioine, the current reports show that the yield of ergothioine is not high, reaching only a few hundred milligrams per liter. Due to the difficulty in synthesis and extraction of ergothioneine, its price is high and its application is limited.

In the prior art, research on thioneine is mostly concentrated in the fields of food, health products and cosmetics. For example, patent CN103181933B discloses a method for preparing a functional oral preparation rich in thioneine, which will be rich in thioneine edible The mycelium is mixed with water, stirred and leached at high temperature, and then concentrated and added with food additives to make a liquid oral preparation. Patent CN109939027A discloses a method for preparing ergothioine-containing cosmetic stock solution by fermentation of Hericium erinaceus. The stock solution has strong anti-oxidation, anti-radiation and anti-inflammatory effects, and can be used as a raw material to be added to water, milk, cream and other cosmetics in.

In the course of experiments, the inventor found that ergothioine has the disadvantages of being easy to decompose when exposed to light and poor stability. The degradation of ergothioine will reduce or even disappear the efficacy. Therefore, it is necessary to find a method that can inhibit the photodegradation of ergothioneine and develop a product with stable efficacy that can be used in food, health products and cosmetics.

Summary of the invention

Aiming at the current problems that thiothione is easily decomposed due to light and its efficacy is unstable, the present invention provides a method that can effectively inhibit the photodegradation of thiothione. The invention also provides an ergothioine-containing composition which has stable efficacy and can be used in food, health care products and cosmetics.

In order to achieve the above objective, the present invention adopts the following technical solutions.

A method for inhibiting the degradation of ergothioine includes the following steps: adding hyaluronate into a solution containing ergothioine.

An ergothioine-containing composition includes ergothioine and hyaluronate.

The content of the ergothioine in the solution or composition is 0.0005 to 0.01 wt%. The content of the hyaluronic acid salt is not less than 0.1 wt%; more preferably not less than 0.5 wt%; even more preferably 0.5-1.2 wt%.

The ergothioine source may be ergothioine extract or ergothioine powder. The ergothioneine extract is liquid or solid. Preferably, the ergothioine content in the liquid ergothioine extract is 50-500 mg/L; the ergothioine content in the solid ergothioine extract is 0.1-0.5 mg/g.

The hyaluronic acid salt is selected from salts that are conventionally used for skin or food, such as at least one of sodium salt, potassium salt, magnesium salt, zinc salt, calcium salt or quaternary ammonium salt.

The molecular weight of the hyaluronate is 3kDa-1800kDa. The molecular weight of the hyaluronate can be in a single range, or a mixture of several different molecular weight ranges; such as high molecular weight hyaluronate (1000kDa-1800kDa), medium molecular weight hyaluronate (500kDa-1000kDa) ), a mix of low molecular weight hyaluronate (10kDa-500kDa) and ultra-low molecular weight hyaluronate (3kDa-10kDa). Preferably, the hyaluronate is 1-3 parts by weight of hyaluronate with a molecular weight of 1200kDa-1600kDa, 2-4 parts by weight of hyaluronate with a molecular weight of 300kDa-800kDa, and hyaluronate with a molecular weight of 3kDa-10kDa Mix of 2-5 parts by weight of acid salt.

The above composition can be prepared by conventional methods. For example, it is formulated into a mother liquor with a certain concentration after dissolution, or the raw material powders are uniformly mixed.

The composition also contains auxiliary materials acceptable in the field of food and health food, such as at least one of solvents, preservatives, thickeners, flavoring agents, flavors and pigments.

The composition also contains auxiliary materials acceptable in the cosmetics field, such as solvents, solubilizers, surfactants, preservatives, antioxidants, pH regulators, penetration enhancers, liposomes, moisturizers, thickeners, chelating agents At least one of a mixture, a skin feel modifier, a surfactant, an emulsifier, a propellant/propellant, a fragrance, a coloring agent, and a sunscreen agent.

The composition also contains other effective ingredients, such as anti-oxidation, anti-ultraviolet, promoting cell proliferation, whitening, anti-inflammatory and other functional ingredients.

The use of the above composition in the preparation of food health products can be directly used for moisturizing and anti-aging, and can also be added to food health products as an active ingredient.

A food and health product containing the above composition. The content of the composition in the food and health product is 0.1-100wt%; preferably 1-20wt%; more preferably 2-10wt%.

The function of the food health product can be a food health product that is moisturizing and anti-aging, anti-inflammatory, joint maintenance or repair, and intestinal and stomach clearing.

The food and health product can be in the form of at least one of beverages, powders, oral liquids, tablets, capsules, and granular solid powders.

The use of one of the above compositions in the preparation of cosmetics can be directly used for skin moisturizing and anti-aging, and can also be added to cosmetics as an active ingredient.

A cosmetic comprising the above composition. The content of the composition in cosmetics is 0.1-100% by weight; preferably 1-20% by weight; more preferably 2-10% by weight.

The cosmetic function can be moisturizing, anti-aging, anti-inflammatory, whitening, sunscreen or isolation cosmetics.

The cosmetic form may be at least one of sunscreen, release cream, skin lotion, toner, skin care gel, skin care lotion, skin cream, essence, eye cream, facial mask or spray.

The invention has the following advantages:

The present invention combines hyaluronate and ergothioine, which can effectively inhibit the photodecomposition of ergothioine. The composition is used in food health products and cosmetics, and has good moisture retention and functional stability.

Description of the drawings

Figure 1 is the influence of sodium hyaluronate concentration and molecular weight on the content of thioneine before and after light exposure;

Figure 2 shows the cell morphology of UV damaged cells treated with 5% of the sample;

Figure 3 shows the morphology of the cells after 5% sample treatment and then UV damage.

Detailed ways

The present invention will be further described below in conjunction with the embodiments and drawings, but the present invention is not limited by the following embodiments.

Example 1 The inhibitory effect of hyaluronic acid on the photodegradation of thioneine

The light conditions (1000 Lux) were used to simulate the degradation of thioneine-containing products during use to determine the inhibitory effect of hyaluronic acid on the photodegradation of thioneine.

1. The effect of sodium hyaluronate with different molecular weights on the photodegradation of thiothione

Add 0.5% sodium hyaluronate with a molecular weight of 1200kDa-1600kDa, sodium hyaluronate with a molecular weight of 300kDa-800kDa, and sodium hyaluronate with a molecular weight of 3kDa-10kDa into the thioneine solution, and stir until the hyaluronic acid is complete After dissolving, add 2% v/v 1,3-butanediol and 3% v/v 1,2-pentanediol, then place it in a transparent plastic bottle, and place it in a light incubator at 25°C. The photoperiod is 24:0 (light/dark). Sampling is taken regularly every day to measure the change of ergothioneine content. The control is a solution without hyaluronic acid.

Ergothioine is determined by high performance liquid chromatography, HPLC conditions: Column: Hypersil ODS C18 column (250mm×4.6mm, particle size 5μm); column temperature: 30℃; mobile phase: acetonitrile-water (3:97) ; Flow rate: 1.0mL/min; Detection wavelength: 254nm; Injection volume: 20μL.

In the solution, the initial thioneine content was 145.4 mg/L. After 5 days of light, the results of the change of thioneine content are shown in Table 1. From the results, when the addition amount of sodium hyaluronate is 0.5%, it can effectively inhibit the photodegradation of ergothioneine, and this inhibitory effect has nothing to do with the molecular weight of hyaluronic acid.

Table 1 The effect of different molecular weight sodium hyaluronate on the photodegradation of thioneine

2. The effect of different concentrations of sodium hyaluronate with a molecular weight of 3kDa-10kDa on the photodegradation of thioneine

Add 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1.0% of sodium hyaluronate with a molecular weight of 3kDa-10kDa to the solution containing ergothioine, stir until the hyaluronic acid is completely dissolved, then add 2%v/v 1,3-butanediol and 3%v/v 1,2-pentanediol, then placed in a transparent plastic bottle, and placed in a light incubator at 25°C with a photoperiod of 24:0 (Light/dark). Regular samples were taken every day to measure the change of ergothioine content. The control was a solution without hyaluronic acid. In the solution, the initial ergothioine content was 145.7 mg/L. After 5 days of light, the results are shown in Table 2.

Table 2 The effect of different concentrations of sodium hyaluronate (Mw=3kDa-10kDa) on the photodegradation of thiothione

3. The effect of different concentrations of sodium hyaluronate with a molecular weight of 300kDa-800kDa on the photodegradation of thiothione

Add 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1.0% of sodium hyaluronate with a molecular weight of 300kDa-800kDa to the thioneine solution, stir until the hyaluronic acid is completely dissolved, then add 2% v/v 1,3-butanediol and 3% v/v 1,2-pentanediol, then place them in a transparent plastic bottle, and place them in a light incubator at 25°C. The photoperiod of the incubator is 24:0 (light /dark). Regular samples were taken every day to measure the change of ergothioine content. The control was a solution without hyaluronic acid. In the solution, the initial ergothioine content was 145.7 mg/L. After 5 days of light, the results are shown in Table 3.

Table 3 The effect of different concentrations of sodium hyaluronate (Mw=300kDa-800kDa) on the photodegradation of thioneine

4. The effect of sodium hyaluronate with different concentrations of 1200kDa-1600kDa on the photodegradation of thioneine

Add 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 1.0% sodium hyaluronate with a molecular weight of 1200kDa-1600kDa to the thioneine solution, stir until the hyaluronic acid is completely dissolved, and then add 2% v/v 1,3-butanediol and 3% v/v 1,2-pentanediol, then place them in a transparent plastic bottle, and place them in a light incubator at 25°C. The photoperiod of the incubator is 24:0 (light /dark). Regular samples were taken every day to measure the change of ergothioine content. The control was a solution without hyaluronic acid. In the solution, the initial ergothioine content was 145.7 mg/L. After 5 days of light, the results are shown in Table 4.

Table 4 The effect of different concentrations of sodium hyaluronate (Mw=1200kDa-1600kDa) on the photodegradation of thioneine

The ergothioine solution containing sodium hyaluronate of different concentrations and molecular weights was irradiated for 5 days, and the remaining amount (%) relative to the added amount was used as the abscissa to plot Figure 1. According to the chart, hyaluronic acid salts of various molecular weights have inhibitory effects on the degradation of thioneine. Within a certain range, the inhibitory effect of hyaluronic acid on the photodegradation of ergothione is directly proportional to the concentration. As the concentration increases, the inhibitory effect increases; when the concentration of hyaluronate reaches 0.6%, the slope of the curve becomes smaller and increases The amplitude tends to be flat.

Example 2 Preparation of ergothioine-containing composition

Weigh different raw materials according to the following ratio:

Prepared according to the following steps:

Take sodium hyaluronate with a molecular weight of 1200kDa-1600kDa and sodium hyaluronate with a molecular weight of 300kDa-800kDa, add water and heat to 85°C and stir to dissolve the sodium hyaluronate. After the sodium hyaluronate is completely dissolved, the temperature is reduced to 45°C, and then added The formula of sodium hyaluronate and thioneine extracts with a molecular weight of 3kDa-10kDa are stirred evenly to obtain sample S1;

Take sodium hyaluronate with a molecular weight of 1200kDa-1600kDa and a molecular weight of 300kDa-800kDa, respectively, add water and heat to 85℃ and stir to dissolve the sodium hyaluronate. After the sodium hyaluronate is completely dissolved, the temperature will be cooled to 45℃, and then add the formula amount of ergot Stir the thioine extract evenly to obtain samples S2 and S3;

Take the sodium hyaluronate and thioneine extracts with a molecular weight of 3kDa-10kDa, add water and stir evenly to dissolve, to obtain sample S4;

Weigh the required amount of thioneine extract and add it to water and stir evenly to obtain sample C1;

According to the ratio of S1, take sodium hyaluronate with a molecular weight of 1200kDa-1600kDa and sodium hyaluronate with a molecular weight of 300kDa-800kDa, add water and heat to 85℃ and stir to dissolve the sodium hyaluronate. After the sodium hyaluronate is completely dissolved, the temperature is reduced to 45 ℃, then add the formula amount of sodium hyaluronate with a molecular weight of 3kDa-10kDa and stir evenly to obtain sample C2.

Example 3 Antioxidant activity of the composition

The samples S1-4 and C1 and C2 prepared in Example 2 were placed in transparent plastic bottles, and were placed in a light incubator (1000 Lux) at 25° C. for 5 days, or stored in the dark for 5 days before use.

1. DPPH free radical scavenging test

The illuminated samples S1-4, C1, C2 and non-illuminated S1, C1, C2 prepared in Example 2 were respectively dissolved in water to prepare solutions with the final concentrations of the above samples of 2.0%, 4.0%, 6.0%, and 8.0% spare. Precisely measure 5.0 mL of the DPPH solution and 5.0 mL of the sample solution prepared in Example 2 with different concentrations, respectively, and place them in a test tube with a stopper, and mix them evenly. Adjust zero with an equal volume of 95% ethanol-water mixed solution. Leave it at room temperature for 30 minutes, measure the absorbance of the solution at 523nm, 3 replicates for each concentration; set up another group to accurately measure 5.0 mL of DPPH solution and 5.0 mL of purified water and mix them as a blank control. The operation is the same as above. The calculation method is as follows:

Table 5 DPPH free radical scavenging activity

From the data in Table 5, it can be seen that the DPPH free radical scavenging rate of S1-S4 of the same concentration after illumination is similar, and S1 is slightly higher than S2-S4, indicating that the molecular weight of hyaluronic acid has little effect on the DPPH free radical scavenging rate of the composition. The antioxidant activity of the composite HA composition is slightly higher than that of the single molecular weight HA composition. Before and after light, the DPPH free radical scavenging rate of hyaluronic acid is almost unchanged; the ergothione is significantly reduced due to light, and after a certain amount of hyaluronic acid is added, the DPPH free radical scavenging rate of the composition is reduced. small. This shows that hyaluronic acid can effectively inhibit the reduction of DPPH free radical scavenging rate of thiothioneine after exposure to light, and maintain the antioxidant activity of thiothioneine.

2. Active oxygen free radical scavenging test

Preparation of sample solution: Prepare 5% concentration of the C1, C2, S1-4 and non-illuminated C1, C2, S1 sample solutions of Example 2 with serum-free DMEM culture solution, and filter them through a 0.22μm filter. bacteria.

Preparation of dichlorofluorescein diacetate (DCFH-DA) probe solution: Dilute DCFH-DA with PBS solution (0.1M, pH 7.4), and add 1mL PBS to 0.375μL.

HaCaT cells in the logarithmic growth phase were seeded in a 12-well culture plate at a density of 5×10 ⁴ cells/mL, 2 mL of cell suspension per well, and routinely cultured in a carbon dioxide incubator at 37° C. and 5% CO _{2 for} 24 hours. Group operations as follows:

(1) In the irradiation group, discard 1mL culture medium, cover with plastic wrap, irradiate UVA with 2000μW/cm ² intensity for 2-3h, UVB with 700μW/cm ² intensity for 7min; discard the old culture medium after irradiation, and add them separately Each sample solution, each well is 2mL;

(2) The injury model group and the irradiation group are operated in the same way. After the irradiation, the old culture medium is discarded and serum-free culture medium is added, each well is 2 mL;

(3) The normal control group was cultured routinely, and the medium was changed at the same time as the irradiation group, and serum-free culture medium was added, each hole was 2mL;

Then, after culturing for 16 hours, discard all the culture medium and wash twice with PBS. Add 1.5 mL of DCFH-DA solution to each well, put it into the cell culture incubator and continue to incubate for 30 minutes, and mix it every 5 minutes to make the probe fully bound. The probe solution was discarded, washed twice with serum-free medium, and 1 mL of serum-free medium was added to each well and incubated at 37°C for 10 min. After washing once with PBS, trypsinize the cells, wash twice with PBS, resuspend in 300μL PBS, use flow cytometer dual-channel detection, filter cells before using the machine, FL1-H channel, and collect 10,000 cells per sample. Calculate the ROS clearance rate based on the signal data acquired by channel 1 (FL1-H), that is, the fluorescence intensity or total fluorescence produced by DCF. Use the average fluorescence intensity to calculate the ROS clearance rate:

Table 6 Effects on the generation of reactive oxygen radicals (ROS)

After UVA+UVB irradiation damage, the level of ROS in the cells was significantly increased compared to the normal cultured cells. Compared with the damage model group, some ROS can be removed after exposure to the sample after UV damage. It can be seen from the data in Table 6 that the sample C2 prepared in Example 2 has a low ROS clearance rate, while C1 and S1-4 have a certain clearance effect on the generated ROS. However, ergothione is cleared of ROS after exposure to light. However, after adding hyaluronic acid to ergothioneine, the ROS clearance rate of the composition before and after light is relatively stable.

Example 4 The effect of the composition on the repair of UV-induced cell damage and the cell's defense against UV damage

Preparation of sample solution: The illuminated and non-illuminated samples S1, C1, C2 prepared in Example 2 were respectively dissolved in serum-free medium to prepare S1 and C1, C2 with final concentrations of 2.0%, 3.0%, 4.0%, and 5.0% The solution is filtered and sterilized with a 0.22μm filter membrane for later use.

1. Repair effect of keratinocyte damage caused by combined irradiation of UVA and UVB

Take the HaCaT cells in the logarithmic growth phase, after trypsinization, adjust the cell density to 1×10 ⁵ /mL, inoculate in a flat-bottomed 96-well cell culture plate, 100μL of cell suspension per well, and place in a carbon dioxide incubator at 37℃, 5% CO ₂ routine culture overnight. 7.2J/cm ² UVA plus 126mJ/cm ² UVB were used to irradiate HaCaT cells, and the test groups are as follows:

Table 7 UV Repair Test Group

Grouping	Approach
normal group	No irradiation, add 100μL serum-free medium
Model group	After UVA+UVB irradiation, add 100μL serum-free medium
test group	After UVA+UVB irradiation, add 100μL sample solution of different concentration

After the experimental group was irradiated, the culture medium was discarded, and 100 μL of the sample solution with a concentration of 2.0%, 3.0%, 4.0%, and 5.0% was added to each well. The old culture medium was discarded in the normal group and the model group, and 100 μL of fresh culture medium was added. Enter the incubator and continue to cultivate for 24h. Add 10μL of WST-1 to each well, put it in the cell culture incubator and continue to incubate for 4h. Measure the light absorption value with a microplate reader at the wavelength of 450nm, and calculate the relative proliferation rate (%) according to the following formula:

Relative proliferation rate (%)=absorbance of test group/absorbance of normal group×100%

Table 8 Relative proliferation rate of HaCaT cells treated with different concentrations of samples after UV damage (%)

Note: * means p<0.05 compared with the model group, ** means p<0.01 compared with the model group.

It can be seen from Table 8 that after UVA and UVB combined irradiation damage, compared with the normal group (100%), the HaCaT cell proliferation rate of the model group decreased by 40.40%, indicating that the model was successful. After adding the sample, compared with the model group, the cell proliferation rate is significantly improved, and in the concentration range of 2.0%-5.0%, the cell proliferation rate increases with the increase of the sample concentration. The hyaluronic acid sample before and after the light is UV There is almost no change in the damage repair ability. The ultraviolet damage repair ability of the ergothione extract after illumination is much lower than that of the non-illuminated ergothione extract, while the ultraviolet damage repair ability of the composition of the ergothione extract and hyaluronic acid before and after illumination is not significantly different. . It shows that hyaluronic acid can effectively inhibit the reduction of ergothione damage repairing ability after exposure to ultraviolet light. After UVA+UVB combined irradiation, the HaCaT cell boundary is unclear, the membrane structure is damaged, and there are many apoptotic cells. After the irradiation, after the sample solution is processed, the HaCaT morphology partly returns to normal, and the apoptotic cells are significantly reduced. Figure 2 shows the UV Cell morphology of damaged cells treated with 5% sample S1.

2. Protection against keratinocyte damage caused by combined UVA and UVB irradiation

Take human skin fibroblasts in logarithmic growth phase, after trypsin digestion, adjust the cell density to 1×10 ⁵ /mL, inoculate in a flat-bottomed 96-well cell culture plate, 100μL of cell suspension per well, and place in a carbon dioxide incubator at 37℃ , 5% CO ₂ routine culture overnight. The culture solution was discarded and the test groups were as follows. Each well of the test group was added with 100 μL of sample solution with a concentration of 2.0%, 3.0%, 4.0%, and 5%. The normal group and the model group were added with equal amounts of serum-free medium and placed in the incubator Continue to culture for 24h.

Table 9 UV protection test group

Grouping	Approach
normal group	Add 100μL serum-free medium without irradiation
Model group	After adding 100μL serum-free medium, UVA+UVB irradiation
test group	After adding 100μL sample solution of different concentration, UVA+UVB irradiation

Human skin fibroblasts were irradiated with 7.2J/cm ² UVA plus 126mJ/cm ² UVB. After irradiation, discard the old culture medium, add serum-free culture medium, and continue culturing for 24 hours, add 10 μL WST-1 to each well, and place it in the cell culture incubator to continue incubating for 4 hours. Measure the light absorption value with a microplate reader at a wavelength of 450nm, and calculate the relative proliferation rate (%) according to the following formula:

Relative proliferation rate (%)=absorbance of test group/absorbance of normal group×100%

Table 10 Relative proliferation rate of cells pretreated with different concentrations of samples and then damaged by ultraviolet rays

Note: * means p<0.05 compared with the model group, ** means p<0.01 compared with the model group.

The results are shown in Table 10. Compared with the normal group (100%), the proliferation rate of human skin fibroblasts in the model group decreased by 45.58% after combined irradiation of UVA and UVB, indicating that the model was successful. Before UV irradiation, human skin fibroblasts were pretreated with different concentrations of samples. Compared with the untreated model group, the cell proliferation rate was significantly increased, indicating that the samples have better protection against UV damage and have a protective effect As the concentration of the sample solution increases, it increases. Similarly, the ergothione extract and the hyaluronic acid composition can effectively inhibit the degradation of the ultraviolet protection ability of ergothione due to light exposure. Figure 3 shows the cell morphology of cells treated with 5% sample S1 and then subjected to UV damage.

Example 5 Preparation of food and health products containing ergothioine composition

1. A beautifying, intestinal and stomachic beverage containing ergothioine composition, which is composed of the following components:

Ingredient wt%: thioneine composition 10, sodium citrate 0.2, honey 0.7, deionized water 89.1; thioneine composition: containing 1.2% potassium hyaluronate with a molecular weight of 1600kDa-1800kDa, thioneine extract 1 % (The content of ergothioneine is 200mg/L), supplemented with water;

Technological process: mixing ergothioine composition, sodium citrate, honey and deionized water, compounding and sterilizing to obtain beverage.

2. A powder containing ergothioine composition for beauty, nourishing and joint maintenance, which is composed of the following components:

Ingredient wt%: thioneine composition 5, chondroitin sulfate 0.5, maltodextrin 10, honey 0.5, deionized water 84; thioneine composition: containing 0.7% calcium hyaluronate with a molecular weight of 300kDa-800kDa, ergot Thiine extract 1.5% (ergothioine content is 150mg/L), supplemented with water;

Process: Mix the ergothioine composition, chondroitin sulfate, maltodextrin, honey and deionized water, heat to 60°C and stir to dissolve the excipients evenly, and spray dry the material liquid at a feed rate of 2000mL/ H, the inlet air temperature is 95°C, the outlet air temperature is 185°C, and the powder is finally obtained.

Example 6 Preparation of cosmetics containing ergothioine composition

1. A moisturizing and anti-aging sunscreen lotion containing ergothioine composition, which is composed of the following components:

Ingredient wt%: Phase A: sodium stearoyl glutamate 1.0, pentaerythritol distearate 2.0, dioctyl carbonate 5.0, dimethyl silicone oil 2.0, ethoxyethyl p-methoxycinnamate 7.5, paraben Ketone 5.0, Homosalicylate 5.0, Homosalate 5.0, Dimerized Linoleyl Alcohol/Dimethyl Carbonate Copolymer 1.0, Sodium Polyacrylate 0.6; Phase B: Ergothioine Composition 10.0, Deionized Water 55.9 , Flavor and preservative 0.1; thioneine composition: containing 0.1% sodium hyaluronate with a molecular weight of 1200kDa-1600kDa, 0.3% sodium hyaluronate with a molecular weight of 300kDa-800kDa, and sodium hyaluronate with a molecular weight of 3kDa-10kDa 0.5%, thioneine extract 1.5% (the content of thioneine is 200mg/L), 1,3-butanediol 2%, 1,2-pentanediol 3%, and water to make up to 100%.

Process: Heating phase A and phase B to 80-85°C respectively. Add phase B to phase A with stirring. Homogenize at 55-60℃, stir and cool to below 30℃.

2. A moisturizing and anti-aging emulsion containing ergothioine composition, consisting of the following components:

Ingredient wt%:

Phase A: appropriate amount of deionized water, Carbo 934 NF 0.30

Phase B: triethanolamine 0.10, deionized water 1.00

Phase C: monoglyceride 5.00, cetyl alcohol 2.00, natural canola oil 2.00, simethicone 1.00

Phase D: Ergothioine composition 15.0, Jimei Ⅱ 0.30, flavor and preservative 0.1

Ergothioine composition: containing 0.2% of sodium hyaluronate with a molecular weight of 1200kDa-1600kDa, 0.4% of sodium hyaluronate with a molecular weight of 300kDa-800kDa, 0.3% of sodium hyaluronate with a molecular weight of 3kDa-10kDa, thioneine extract 0.5% (ergothioine content is 400mg/L), 1,3-butanediol 2%, 1,2-pentanediol 3%, water supplement.

Process: Heat phase A to 75°C, stir evenly, add phase B. In another container, heat phase C to 75-78°C, add phase C to phases A and B under vigorous stirring, and stir to form an emulsion. Cool to room temperature. Add the ergothioine composition at 45°C, and add preservatives and flavors at 35°C.

3. A moisturizing and anti-aging toner containing ergothioine composition, which is composed of the following components:

Ingredient wt%:

Phase A: glycerin 5.0, propylene glycol 3.0, oat extract 2.0, benzophenone-4/benzophenone-5 0.5, containing ergothioine composition 9.4, deionized water to 100

Thioine composition: containing 0.1% sodium hyaluronate with a molecular weight of 1200kDa-1600kDa, 0.4% sodium hyaluronate with a molecular weight of 300kDa-800kDa, 0.4% sodium hyaluronate with a molecular weight of 3kDa-10kDa, thionin extract Liquid 1% (the content of ergothioneine is 200mg/L), 1,3-butanediol 2%, 1,2-pentanediol 3%, water supplement.

Phase B: Azone/PEG40 hydrogenated castor oil 1.0, hydrolyzed protein 0.5, triethanolamine 0.25.

Process: Heat phase A and phase B to 80-85°C respectively, after mixing the two phases for a few minutes, stir and cool down, add appropriate amount of flavor and preservative to 45°C, stir evenly, continue to cool to 35°C and stir evenly. .

Claims (10)

1. A method for inhibiting the photodegradation of ergothioneine is characterized in that it comprises the following steps: adding hyaluronate to a solution containing ergothioneine.
2. A composition containing ergothioine, which is characterized by comprising ergothioine and hyaluronate.
3. The method according to claim 1 or the composition according to claim 2, wherein the content of the ergothioine in the solution or composition is 0.0005 to 0.01 wt%.
4. The method of claim 1 or the composition of claim 2, wherein the content of the hyaluronate in the solution or composition is not less than 0.1 wt%; more preferably, it is not less than 0.5 wt% ; More preferably 0.5-1.2wt%.
5. The method of claim 1 or the composition of claim 2, wherein the hyaluronate is selected from at least one of sodium salt, potassium salt, magnesium salt, zinc salt, calcium salt, or quaternary ammonium salt. Kind.
6. The method of claim 1 or the composition of claim 2, wherein the molecular weight of the hyaluronate is 3kDa-1800kDa.
7. The method of claim 1 or the composition of claim 2, wherein the hyaluronate is hyaluronate with a molecular weight of 1200kDa-1600kDa, 1-3 parts by weight and a molecular weight of 300kDa-800kDa 2-4 parts by weight of hyaluronic acid salt and 2-5 parts by weight of hyaluronic acid salt with a molecular weight of 3kDa-10kDa.
8. The composition according to claim 2, characterized in that, the composition further comprises auxiliary materials or active ingredients acceptable in the field of food, health food or cosmetics.
9. A food, health food or cosmetic product comprising the composition according to any one of claims 1-6.
10. The food, health food or cosmetic according to claim 7, characterized in that the content of the composition is 0.1-100wt%; preferably 1-20wt%; more preferably 2-10wt%.

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