WO2020127726A3 - Mikroskop - Google Patents

Mikroskop Download PDF

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Publication number
WO2020127726A3
WO2020127726A3 PCT/EP2019/086299 EP2019086299W WO2020127726A3 WO 2020127726 A3 WO2020127726 A3 WO 2020127726A3 EP 2019086299 W EP2019086299 W EP 2019086299W WO 2020127726 A3 WO2020127726 A3 WO 2020127726A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
unit
beam path
sample
microscope
Prior art date
Application number
PCT/EP2019/086299
Other languages
English (en)
French (fr)
Other versions
WO2020127726A2 (de
Inventor
Werner Knebel
Florian Fahrbach
Original Assignee
Leica Microsystems Cms Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Microsystems Cms Gmbh filed Critical Leica Microsystems Cms Gmbh
Priority to JP2021535934A priority Critical patent/JP2022514666A/ja
Priority to US17/415,761 priority patent/US20220113523A1/en
Priority to EP19835647.9A priority patent/EP3899630A2/de
Publication of WO2020127726A2 publication Critical patent/WO2020127726A2/de
Publication of WO2020127726A3 publication Critical patent/WO2020127726A3/de

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/10—Beam splitting or combining systems
    • G02B27/14—Beam splitting or combining systems operating by reflection only
    • G02B27/141—Beam splitting or combining systems operating by reflection only using dichroic mirrors
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Die Erfindung betrifft ein Mikroskop (10) mit einer Weitfeld-Beleuchtungseinheit (12) zur Beleuchtung zumindest eines ausgewählten Bereichs einer Probe (14) und mit einer Strahlteilereinheit (16, 16') zur Erzeugung eines ersten Detektionsstrahlengangs (18) und eines zweiten Detektionsstrahlengangs (20). Das Mikroskop (10) umfasst ferner eine innerhalb des ersten Detektionsstrahlengangs (18) angeordnete Kamera-Detektionseinheit (20) zur Aufnahme von Bildern des ausgewählten Bereichs der Probe (14) und eine innerhalb des zweiten Detektionsstrahlengangs (20) angeordnete Punkt-Detektionseinheit (24) zur Erfassung eines innerhalb des ausgewählten Bereichs liegenden vorbestimmten Teilbereichs der Probe (14). Innerhalb des ersten und zweiten Detektionsstrahlengangs (18, 20) ist objektseitig der Strahlteilereinheit (16, 16') ein Detektionsobjektiv (26) angeordnet, das als gemeinsames Detektionsobjektiv für die Kamera- Detektionseinheit (22, 22') und die Punkt-Detektionseinheit (24) vorgesehen ist.
PCT/EP2019/086299 2018-12-21 2019-12-19 Mikroskop WO2020127726A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2021535934A JP2022514666A (ja) 2018-12-21 2019-12-19 顕微鏡
US17/415,761 US20220113523A1 (en) 2018-12-21 2019-12-19 Microscope
EP19835647.9A EP3899630A2 (de) 2018-12-21 2019-12-19 Mikroskop

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102018133509 2018-12-21
DE102018133509.1 2018-12-21
DE102019110869.1 2019-04-26
DE102019110869.1A DE102019110869A1 (de) 2018-12-21 2019-04-26 Mikroskop

Publications (2)

Publication Number Publication Date
WO2020127726A2 WO2020127726A2 (de) 2020-06-25
WO2020127726A3 true WO2020127726A3 (de) 2020-08-27

Family

ID=69159730

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2019/086299 WO2020127726A2 (de) 2018-12-21 2019-12-19 Mikroskop

Country Status (5)

Country Link
US (1) US20220113523A1 (de)
EP (1) EP3899630A2 (de)
JP (1) JP2022514666A (de)
DE (1) DE102019110869A1 (de)
WO (1) WO2020127726A2 (de)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5377003A (en) * 1992-03-06 1994-12-27 The United States Of America As Represented By The Department Of Health And Human Services Spectroscopic imaging device employing imaging quality spectral filters
DE102010035003A1 (de) * 2010-08-20 2012-02-23 PicoQuant GmbH. Unternehmen für optoelektronische Forschung und Entwicklung Räumlich und zeitlich hochauflösende Mikroskopie

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5528368A (en) * 1992-03-06 1996-06-18 The United States Of America As Represented By The Department Of Health And Human Services Spectroscopic imaging device employing imaging quality spectral filters
US5479252A (en) * 1993-06-17 1995-12-26 Ultrapointe Corporation Laser imaging system for inspection and analysis of sub-micron particles
EP0911667B1 (de) * 1997-10-22 2003-04-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Programmierbares räumlich lichtmoduliertes Mikroskop und Mikroskopieverfahren
EP1207415B1 (de) * 1997-10-29 2006-08-30 MacAulay, Calum, E. Gerät und Verfahren zur Mikroskopie unter Verwendung räumlich modulierten Lichtes
DE19835072A1 (de) * 1998-08-04 2000-02-10 Zeiss Carl Jena Gmbh Anordnung zur Beleuchtung und/oder Detektion in einem Mikroskop
DE20216583U1 (de) 2001-12-20 2003-01-23 Leica Microsystems Mikroskop und Durchflusszytometer
US20040133112A1 (en) * 2002-03-08 2004-07-08 Milind Rajadhyaksha System and method for macroscopic and confocal imaging of tissue
DE10309138A1 (de) * 2003-02-28 2004-09-16 Till I.D. Gmbh Mikroskopvorrichtung
JP4716686B2 (ja) * 2004-07-23 2011-07-06 オリンパス株式会社 顕微鏡装置
CN101498833A (zh) * 2009-03-06 2009-08-05 北京理工大学 兼有宏-微视场观测的超分辨差动共焦显微镜
JP5307629B2 (ja) * 2009-05-22 2013-10-02 オリンパス株式会社 走査型顕微鏡装置
JP2010286565A (ja) * 2009-06-09 2010-12-24 Olympus Corp 蛍光観察装置
DE102012211943A1 (de) * 2012-07-09 2014-06-12 Carl Zeiss Microscopy Gmbh Mikroskop
DE102012214568A1 (de) * 2012-08-16 2014-02-20 Leica Microsystems Cms Gmbh Optische Anordnung und ein Mikroskop
DE102012020240A1 (de) * 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur SPIM Mikroskopie
US9500846B2 (en) * 2014-03-17 2016-11-22 Howard Hughes Medical Institute Rapid adaptive optical microscopy over large multicellular volumes
CN103926228B (zh) * 2014-04-28 2016-03-02 江苏天宁光子科技有限公司 一种激光扫描共焦荧光显微内窥成像系统
DE102018124129A1 (de) * 2017-12-04 2019-06-06 Leica Microsystems Cms Gmbh Mikroskopsystem und Verfahren zur mikroskopischen Abbildung mit einem solchen Mikroskopsystem

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5377003A (en) * 1992-03-06 1994-12-27 The United States Of America As Represented By The Department Of Health And Human Services Spectroscopic imaging device employing imaging quality spectral filters
DE102010035003A1 (de) * 2010-08-20 2012-02-23 PicoQuant GmbH. Unternehmen für optoelektronische Forschung und Entwicklung Räumlich und zeitlich hochauflösende Mikroskopie

Also Published As

Publication number Publication date
US20220113523A1 (en) 2022-04-14
JP2022514666A (ja) 2022-02-14
DE102019110869A1 (de) 2020-06-25
EP3899630A2 (de) 2021-10-27
WO2020127726A2 (de) 2020-06-25

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