WO2020119600A1 - Image acquisition device and detection apparatus for particulate matter in liquid - Google Patents

Image acquisition device and detection apparatus for particulate matter in liquid Download PDF

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Publication number
WO2020119600A1
WO2020119600A1 PCT/CN2019/123649 CN2019123649W WO2020119600A1 WO 2020119600 A1 WO2020119600 A1 WO 2020119600A1 CN 2019123649 W CN2019123649 W CN 2019123649W WO 2020119600 A1 WO2020119600 A1 WO 2020119600A1
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WIPO (PCT)
Prior art keywords
liquid
container
image acquisition
particulate matter
acquisition device
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PCT/CN2019/123649
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French (fr)
Chinese (zh)
Inventor
李剑平
陈涛
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中国科学院深圳先进技术研究院
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Publication of WO2020119600A1 publication Critical patent/WO2020119600A1/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/144Imaging characterised by its optical setup

Definitions

  • the present invention relates to the technical field of detection of particulate matter in liquid, and in particular to image acquisition equipment and detection device for particulate matter in liquid.
  • the optical imaging method is characterized by its non-contact nature and the ability to quickly obtain high-resolution images of particulate matter. It has broad application prospects in the study of plankton, small aquatic organisms and microplastics in liquids.
  • an optical imaging system such as a flow microscopic imaging system is often used in the detection equipment for particles in a liquid, and a light-transmitting sample flow cell is provided for the liquid with particles to flow in the sample flow cell, and Set the light source to enter the light from the side that irradiates the sample flow cell, so that the light passing through the liquid in the sample flow cell enters the camera for imaging, and then use a computer or other image analysis equipment to analyze the image taken by the camera to obtain information on the corresponding particulate matter .
  • the liquid in the sample flow cell is located on the object-side focal plane of the camera, and the flow direction of the liquid is parallel to the object-side focal plane of the camera.
  • the object of the present invention is to provide an image acquisition device and a detection device for particles in a liquid to solve the above-mentioned problems.
  • An image acquisition device for particles in a liquid includes: a liquid measurement container, a light source, and a shooting device, wherein the liquid measurement container is used to contain a liquid with particulate matter, and the liquid is in the liquid measurement container The light flows in a specific flow direction; the light source is used to irradiate the liquid to cause light scattering of the particles in the liquid; the shooting device, the direction of the optical axis of the shooting device coincides with the specific flow direction, so The photographing device is used to photograph an image of particulate matter where light scattering occurs in the liquid.
  • the liquid measuring container includes a temporary storage tube and a sampling tube provided in the temporary storage tube, a predetermined distance between the bottom end of the sampling tube and the bottom end of the temporary storage tube, the The bottom end of the sampling tube is open to communicate with the temporary storage tube; wherein, the liquid flows into the temporary storage tube through the bottom end of the sampling tube.
  • the light center plane of the light source coincides with the object-side focal plane of the shooting device.
  • the plane of the bottom end of the light beam emitted by the light source coincides with the plane of the bottom end of the sampling tube.
  • the image acquisition device further includes a liquid supply container in communication with the liquid measurement container, and the liquid supply container is used to supply the liquid with particulate matter to the liquid measurement container.
  • the image acquisition device further includes a pressure pump in communication with the bottom end of the liquid supply container and a liquid augmentation container in communication with the pressure pump, the pressure pump is used to transfer the liquid in the liquid augmentation container Inject into the liquid supply container.
  • the image acquisition device further includes: a negative pressure pump and a waste liquid container communicating with the bottom end of the liquid measuring container, the negative pressure pump communicating with the waste liquid container to adjust the waste liquid container The air pressure inside.
  • the waste liquid container includes a plurality of child waste liquid containers, and the plurality of child waste liquid containers communicate with each other.
  • the shooting device includes a telecentric lens, and the depth of field of the telecentric lens is within the illumination range of the light source in the specific flow direction.
  • the present invention also provides a device for detecting particulate matter in a liquid.
  • the detection device includes an image analysis device and an image acquisition device as described above.
  • the image analysis device is configured to receive an image of the particulate matter captured by the shooting device, and The analysis is based on the image of the particulate matter.
  • the image acquisition device and detection device for particulate matter in a liquid provided by the present invention, by setting a sample measuring container to make the liquid as a sample flow in a specific flow direction, and setting a light source to irradiate the particulate matter in the liquid to cause light scattering of the particulate matter, A photographing device whose optical axis direction coincides with the specific flow direction collects an image of the light-scattering particulate matter for analysis by the image analysis device. It effectively solves the problem that the speed of the particulate matter in the flow direction is too fast and the image of the particulate matter is smeared, which makes it possible to use the image acquisition device to collect the image of the particulate matter without lifting the conditions.
  • the above scheme uses dark field imaging to improve the contrast and signal-to-noise ratio of the obtained particulate image, and based on imaging the particulate in the fluid, it has the advantages of not being affected by the type of particulate in the liquid and adapting to the environment with large external disturbances. .
  • FIG. 1 is a schematic structural diagram of an image acquisition device for particulate matter in a liquid provided by an embodiment of the present invention
  • FIG. 2 is an exemplary optical path diagram of a light source on one side of the image acquisition device illuminating the liquid in the liquid measuring container;
  • FIG. 3 is an exemplary layout diagram of the light source
  • FIG. 4 is an exemplary connection diagram of sub-liquid waste containers in the waste liquid container.
  • this embodiment provides an image acquisition device for particles in a liquid.
  • the image acquisition device includes a liquid measuring container 1, a light source 2, and a shooting device 3.
  • the liquid measuring container 1 is used for containing liquid with particulate matter, and the liquid flows in the liquid measuring container 1 along a specific flow direction;
  • the light source 2 is used to irradiate the liquid to cause light scattering of the particles in the liquid;
  • the direction of the optical axis of the photographing device 3 coincides with the specific flow direction, and the photographing device 3 is used for photographing an image of particulate matter in which light scattering occurs in the liquid.
  • An image acquisition device for particles in a liquid uses the principle of dark field illumination imaging to make the liquid in the liquid measuring container 1 flow as a sample in a specific flow direction, and the particles in the liquid flow through Light scattering occurs in the light range of the light source 2, and the image of the light-scattered particles is collected by the shooting device 3, so that the image analysis equipment 7 such as a computer can analyze the image to obtain the shape, size, and Information such as concentration and particle size distribution.
  • the image acquisition device is based on the principle of dark field imaging, which can not only obtain the detailed information of the texture of the particulate matter and flagella, but also have the advantages of improving the contrast and granularity of the acquired particulate image.
  • the image acquisition device is suitable for particles with a size range of 500 ⁇ m to 20 mm.
  • the image acquisition device is based on the principle of fluid imaging and has the advantage of not being affected by the type of particles in the liquid. It can not only perform suspended particles without swimming ability Imaging can also image living plankton with strong swimming ability. In addition, it does not need to wait for the particulate matter to settle to the bottom of the liquid measurement container to collect images. The external interference has little effect on the normal operation of the image collection device.
  • the image acquisition device can be applied to on-site environments such as floating row and scientific research ship.
  • the liquid measuring container 1 includes a temporary storage tube 12 and a sampling tube 11 disposed in the temporary storage tube 12, the bottom end of the sampling tube 11 and the bottom end of the temporary storage tube 12 There is a predetermined distance between them, and the bottom end of the sampling tube 11 is open to communicate with the temporary storage tube 12; wherein, the liquid flows into the temporary storage tube 12 via the bottom end of the sampling tube 11.
  • the liquid can flow out from the bottom end of the sampling tube 11 under the obstruction of the bottom end of the temporary storage tube 12 From the bottom end of the temporary storage tube 12 to the surroundings, the liquid that has undergone image acquisition quickly moves away from the field of view of the shooting device 3, avoiding it from affecting the captured image.
  • the predetermined distance between the bottom end of the sampling tube 11 and the bottom end of the temporary storage tube 12 needs to be determined according to the maximum particle size of the captured particulate matter.
  • the predetermined distance is set equal to the particle size of the largest particulate matter.
  • the sampling tube 11 and the temporary storage tube 12 are highly transparent containers.
  • the light source 2 uses a light source with a small divergence angle, and the light center plane 20 of the light source coincides with the object-side focal plane of the shooting device 3.
  • the light center plane 20 is located at the center of the exemplary light paths a and b on both sides of the edge of the illumination range, and the object-side focal plane of the shooting device 3 coincides with the light center plane 20,
  • the depth of field of the shooting device 3 is matched with the illumination range of the light source 2, which can ensure that the particles within the depth of field of the shooting device 3 are all within the illumination range of the light source 2, so that a clear image of particles can be captured.
  • the optical path of the light generated by the light source 2 perpendicular to the optical axis of the shooting device 3, the light generated by the light source 2 is prevented from entering the shooting device 3 and affecting the captured image.
  • the light source 2 includes more than two illuminators 21 that are arranged around the liquid measuring container 1 at an equal angle with each other. In this embodiment, three strips are selected.
  • the illuminator 21 is embedded in the light source bracket, so that the three illuminators 21 surround the liquid measuring container 1, so that the illumination range of the three illuminators 21 completely covers the liquid measuring container 1
  • the cross-section of the test area for example, as shown in FIG. 3, if the sampling tube 11 of the liquid measuring container 1 selects a cylindrical tubular container, the three illuminators 21 produce overlapping areas of the illumination range The area is larger than the cross-sectional area of the sampling tube 11, and all positions on the edge of the sampling tube 11 are within the illumination range.
  • the shooting device 3 needs to be exposed for a short time, so the illuminator 21 needs to use a high-intensity illuminator.
  • the light source 2 may be a monochromatic light source or a color light source composed of at least three illuminators 21 corresponding to the three primary colors, which are respectively used to obtain a grayscale image or a color image of the particulate matter.
  • the plane of the bottom end of the light beam emitted by the light source 2 coincides with the plane of the bottom end of the sampling tube 11. Prevent the phenomenon that unilluminated particles block the imaging, and improve the signal-to-noise ratio and contrast of the image.
  • the shooting device 3 includes a telecentric lens 31 whose depth of field matches the illumination range of the light source 2 in the specific flow direction, preferably, the depth of field of the telecentric lens 31 It coincides with the light range of the light source 2 in the specific flow direction.
  • the telecentric lens 31 can make the magnification of the image acquired in the depth of field range the same.
  • the depth of field matches the illumination range, even if the light scattering particles in the liquid are at different heights in the sampling tube 11, the shooting In the image taken by the device 3 through the telecentric lens 31, the imaging magnification of all particles is the same, and there will be no deviation due to the difference in distance from the telecentric lens 31, thereby avoiding the particles caused by the above reasons. Has an error in the size information.
  • the light source 2 is configured as the above-mentioned light source composed of three strip-shaped illuminators
  • the two focusing planes used to define the depth of field range of the telecentric lens 31 are respectively
  • the optical paths a and b coincide.
  • the image acquisition device further includes a liquid supply container 4 communicating with the liquid measuring container 1, and the liquid supply container 4 is used to supply the liquid with particulate matter to the liquid measuring container 1.
  • the liquid supply container 4 is in communication with the top of the sampling tube 11 of the liquid measuring container 1.
  • the liquid supply container 4 is a container whose volume is much larger than that of the sampling tube 11, so that a sufficient amount of Liquid for high-throughput imaging.
  • the image acquisition device further includes a pressure pump 51 communicating with the bottom end of the liquid supply container 4 and a liquid increasing container 5 communicating with the pressure pump 51, and the pressure pump 51 is used to The liquid in the liquid increasing container 5 is injected into the liquid supply container 4.
  • the pressure pump 51 injects the liquid in the liquid supply container 4 from the bottom end of the liquid supply container 4, on the one hand, the liquid at the bottom of the liquid supply container 4 flows to prevent the liquid supply container 4 from The particulate matter settles on the bottom of it, avoiding the concentration of particulate matter entering the sample tube 11 and affecting the imaging, thereby avoiding affecting the accuracy of the detection results; on the other hand, by supplementing the liquid supply container 4 with liquid, it can be controlled
  • the liquid level in the liquid supply container 4 further controls the speed of the liquid flowing in the sampling tube 11.
  • the liquid can be selected from pure water, pure sea water, and liquid as a sample, which does not interfere with particulate matter.
  • the image acquisition device further includes a rotary blade immersed in the bottom of the liquid supply container 4 and a motor electrically connected to the rotary blade, the motor is used to drive the rotary blade in The liquid supply container 4 rotates to disturb the liquid at the bottom of the liquid supply container 4.
  • the image acquisition device further includes: a negative pressure pump 61 and a waste liquid container 6 communicating with the bottom end of the liquid measuring container 1.
  • the waste liquid container 6 is a closed container, which has a good sealability, so
  • the negative pressure pump 61 communicates with the waste liquid container 6 to adjust the air pressure in the waste liquid container 6, and the air pressure in the waste liquid container 6 can be used to indirectly regulate the flow of liquid in the liquid measurement container 1 Speed, to avoid damage to vulnerable particles in the liquid, such as sea snow and other samples.
  • the liquid collected in the waste liquid container 6 can be used for recycling to avoid the waste of samples.
  • the volume of the waste liquid container 6 needs to be large enough.
  • the waste liquid container 6 includes a plurality of sub-waste liquid containers 60, and the plurality of sub-waste liquid containers 60 are connected to each other according to a certain rule, for example, by serial connection in series or by one sub-waste
  • the liquid container 60 is connected in parallel with a plurality of child waste liquid containers 60 and the like to increase the volume of the waste liquid container 6 by increasing the number of the connected child waste liquid containers 60.
  • the image acquisition device for the particulate matter in the liquid further includes a bracket 8 to fix the liquid measurement container 1, the light source 2, the photographing device 3, and the liquid supply container 4 at corresponding positions of the bracket 8 so that the liquid measurement container 1
  • the extension direction of is consistent with the direction of gravity, and the liquid can naturally flow along the specific flow direction under the action of gravity.
  • the bracket 8 is selected from a light-weight and high-strength aluminum bracket, and each assembly position on the bracket 8 is adjustable.
  • the driving device may also drive the liquid to flow in the specific flow direction.
  • An exemplary use process of the image acquisition device based on the particulate matter in the liquid is as follows: First, pure sea water is injected into the sample container 1 through the liquid supply container 4 until the sea water does not pass through the sampling tube 11 of the sample container 1 Corresponds to the depth of field of the shooting device 3; then, the liquid supply container 4 injects the liquid as the sample to be tested into the sampling tube 11 of the sample container 1, so that the shooting device 3 performs shooting and passes the control
  • the pump valve causes the liquid to flow.
  • pure sea water is injected into the sample container 1 again until the image captured by the shooting device 3 No more particles appear, stop shooting and close the pump valve to complete the image acquisition.
  • the present invention also provides a device for detecting particulate matter in a liquid.
  • the detection device includes an image analysis device 7 and an image acquisition device as described above.
  • the image analysis device 7 is configured to receive particles captured by the shooting device 3. Image, and analyze based on the image of the particulate matter.
  • the components in the above-mentioned device for detecting particulate matter in a liquid can be divided into the following modules: an imaging unit, a sampling unit, a disturbance unit, a fluid control unit, and an image analysis unit, wherein the imaging unit is composed of the light source 2 and the shooting device 3, the sampling unit is composed of the liquid measuring container 1 and the liquid supply container 4, the disturbance unit is composed of the pressure pump 51 and the liquid increase container 5, the fluid control unit is composed of The negative pressure pump 61, the waste liquid container 6, the liquid supply container 4, the pressure pump 51, and the booster container 5 are configured, and the image analysis unit is configured by the image analysis device.
  • the imaging unit is composed of the light source 2 and the shooting device 3
  • the sampling unit is composed of the liquid measuring container 1 and the liquid supply container 4
  • the disturbance unit is composed of the pressure pump 51 and the liquid increase container 5
  • the fluid control unit is composed of The negative pressure pump 61, the waste liquid container 6, the liquid supply container 4, the pressure pump 51, and the booster container 5 are configured
  • the image analysis unit
  • the frame rate of the shooting device 3 and the flow rate of the liquid in the sampling tube 11 follow the relationship between the following parameters:
  • f is the frame rate of the shooting device 3
  • v is the flow rate of the liquid in the sampling tube 11
  • 1 is the depth of field of the shooting device 3
  • s is the cross-sectional area of the sampling tube 11, ie
  • the frame rate of the shooting device 3 is equal to the flow rate of the liquid in the sampling tube 11 divided by the product of the depth of field of the shooting device 3 and the cross-sectional area of the sampling tube 11.
  • the frame rate f of the shooting device 3 and the flow rate v of the liquid in the sampling tube 11 are adjusted to ensure the measurement data Accuracy, and according to this formula, after setting the parameters of one of the frame rate f of the shooting device 3 and the flow rate v of the liquid in the sampling tube 11, the other party can be fed back according to the above relationship Parameters.
  • the image acquisition device and detection device for particulate matter in liquid provided by the present invention, by providing a sample measuring container 1 to allow the liquid as a sample to flow in a specific flow direction, and a light source 2 to irradiate particulate matter in the liquid
  • the imaging device 3 whose optical axis direction coincides with the specific flow direction collects an image of the light scattering particulate matter for analysis by the image analysis device 7. It effectively solves the problem that the speed of the particulate matter in the flow direction is too fast and the image of the particulate matter is smeared, which makes it possible to use the image acquisition device to collect the image of the particulate matter without lifting the conditions.
  • the above scheme uses dark field imaging to improve the contrast and signal-to-noise ratio of the obtained particulate image, and based on imaging the particulate in the fluid, it has the advantages of not being affected by the type of particulate in the liquid and adapting to the environment with large external disturbances. .

Abstract

Provided are an image acquisition device and a detection apparatus for particulate matter in liquid. The image acquisition device comprises a detection liquid container (1), a light source (2), and a photographing device (3). The detection liquid container (1) is used for accommodating a liquid having particulate matter, and the liquid flows in a specific flow direction in the detection liquid container (1); the light source (2) is used for irradiating the liquid, so that light scatters on the particulate matter in the liquid; and the photographing device (3) is used for photographing image of the particulate matter, on which light scatters, in the liquid; the direction of the optical axis coincides with the specific flow direction, avoiding streaking and blurring of an image due to the speed of the the particulate matter in the flow direction being too high, reducing the influence of streaking and blurring on said acquisition device, thereby improving the flow speed of the particulate matter, improving the imaging flux, and improving the detection efficiency. The dark-field imaging manner can improve the contrast and signal-to-noise ratio of the image of the particulate matter, and performs imaging on the basis of particulate matter in a fluid, thus having the advantages of not being affected by the type of particulate matter and adapting to an external environment having a great disturbance.

Description

液体中颗粒物的图像采集设备及检测装置Image acquisition equipment and detection device for particles in liquid 技术领域Technical field
本发明涉及液体中颗粒物的检测技术领域,尤其涉及液体中颗粒物的图像采集设备及检测装置。The present invention relates to the technical field of detection of particulate matter in liquid, and in particular to image acquisition equipment and detection device for particulate matter in liquid.
背景技术Background technique
光学成像方法以其非接触性和能够快速得到颗粒物的高分辨率图像的特点,在液体中的浮游生物、小型水生物以及微塑料等颗粒物研究上具有广阔的应用前景。The optical imaging method is characterized by its non-contact nature and the ability to quickly obtain high-resolution images of particulate matter. It has broad application prospects in the study of plankton, small aquatic organisms and microplastics in liquids.
现有技术中,液体中颗粒物的检测设备常常采用流式显微成像系统一类的光学成像系统,通过设置透光的样品流通池以供带有颗粒物的液体在样品流通池中进行流动,并设置光源从照射样品流通池的一侧射入光线,使通过样品流通池内的液体的光线进入摄像机中进行成像,再利用计算机等图像分析设备对摄像机拍摄的图像进行分析即可获取对应颗粒物的信息。其中,在样品流动池中的液体位于摄像机的物方焦平面上,且液体的流动方向平行于摄像机的物方焦平面。因此,当样品流动池中液体的流速过快时,颗粒物对应地在摄像机的物方焦平面上的位移速度过快,很容易使得摄像机获取的图像出现颗粒物的拖尾模糊现象,使得图像分析设备无法正确地解析颗粒物的信息。而若对液体的流速进行限制,则会由于通量过低而降低了检测设备的工作效率,难以对大量的液体进行解析。In the prior art, an optical imaging system such as a flow microscopic imaging system is often used in the detection equipment for particles in a liquid, and a light-transmitting sample flow cell is provided for the liquid with particles to flow in the sample flow cell, and Set the light source to enter the light from the side that irradiates the sample flow cell, so that the light passing through the liquid in the sample flow cell enters the camera for imaging, and then use a computer or other image analysis equipment to analyze the image taken by the camera to obtain information on the corresponding particulate matter . Wherein, the liquid in the sample flow cell is located on the object-side focal plane of the camera, and the flow direction of the liquid is parallel to the object-side focal plane of the camera. Therefore, when the flow velocity of the liquid in the sample flow cell is too fast, the displacement speed of the particles on the focal plane of the object side of the camera is too fast, it is easy to cause the smearing of particles in the image acquired by the camera, which makes the image analysis equipment The information of the particulate matter cannot be correctly parsed. If the flow rate of the liquid is limited, the working efficiency of the detection device will be reduced due to the low flux, and it is difficult to analyze a large amount of liquid.
发明内容Summary of the invention
有鉴于此,本发明的目的在于提供液体中颗粒物的图像采集设备及检测装置,来解决上述问题。In view of this, the object of the present invention is to provide an image acquisition device and a detection device for particles in a liquid to solve the above-mentioned problems.
为了实现上述的目的,本发明采用了如下的技术方案:In order to achieve the above objectives, the present invention adopts the following technical solutions:
一种液体中颗粒物的图像采集设备,所述图像采集设备包括:测液容器、光源以及拍摄设备,其中,所述测液容器,用于容置具有颗粒物的液体,液体在所述测液容器内沿特定流动方向进行流动;所述光源用于对液体进行照射, 以使液体内的颗粒物发生光散射;所述拍摄设备,所述拍摄设备的光轴方向与所述特定流动方向重合,所述拍摄设备用于拍摄液体内发生光散射的颗粒物的图像。An image acquisition device for particles in a liquid, the image acquisition device includes: a liquid measurement container, a light source, and a shooting device, wherein the liquid measurement container is used to contain a liquid with particulate matter, and the liquid is in the liquid measurement container The light flows in a specific flow direction; the light source is used to irradiate the liquid to cause light scattering of the particles in the liquid; the shooting device, the direction of the optical axis of the shooting device coincides with the specific flow direction, so The photographing device is used to photograph an image of particulate matter where light scattering occurs in the liquid.
优选地,所述测液容器包括暂存管和设置于所述暂存管内的进样管,所述进样管的底端与所述暂存管的底端之间具有预定距离,所述进样管的底端开放,以与所述暂存管连通;其中,液体经由所述进样管的底端流入到所述暂存管。Preferably, the liquid measuring container includes a temporary storage tube and a sampling tube provided in the temporary storage tube, a predetermined distance between the bottom end of the sampling tube and the bottom end of the temporary storage tube, the The bottom end of the sampling tube is open to communicate with the temporary storage tube; wherein, the liquid flows into the temporary storage tube through the bottom end of the sampling tube.
优选地,所述光源的光线中心面与所述拍摄设备的物方焦平面重合。Preferably, the light center plane of the light source coincides with the object-side focal plane of the shooting device.
优选地,所述光源所发出光束的底端所在平面与所述进样管的底端所在平面重合。Preferably, the plane of the bottom end of the light beam emitted by the light source coincides with the plane of the bottom end of the sampling tube.
优选地,所述图像采集设备还包括与所述测液容器连通的供液容器,所述供液容器用于向所述测液容器提供具有颗粒物的液体。Preferably, the image acquisition device further includes a liquid supply container in communication with the liquid measurement container, and the liquid supply container is used to supply the liquid with particulate matter to the liquid measurement container.
优选地,所述图像采集设备还包括与所述供液容器的底端连通的压力泵以及与所述压力泵连通的增液容器,所述压力泵用于将所述增液容器内的液体注射至所述供液容器内。Preferably, the image acquisition device further includes a pressure pump in communication with the bottom end of the liquid supply container and a liquid augmentation container in communication with the pressure pump, the pressure pump is used to transfer the liquid in the liquid augmentation container Inject into the liquid supply container.
优选地,所述图像采集设备还包括:负压泵和与所述测液容器的底端连通的废液容器,所述负压泵与所述废液容器连通,以调节所述废液容器内的气压。Preferably, the image acquisition device further includes: a negative pressure pump and a waste liquid container communicating with the bottom end of the liquid measuring container, the negative pressure pump communicating with the waste liquid container to adjust the waste liquid container The air pressure inside.
优选地,所述废液容器包括多个子废液容器,所述多个子废液容器彼此连通。Preferably, the waste liquid container includes a plurality of child waste liquid containers, and the plurality of child waste liquid containers communicate with each other.
优选地,所述拍摄设备包括远心镜头,所述远心镜头的景深在所述光源于所述特定流动方向上的光照范围内。Preferably, the shooting device includes a telecentric lens, and the depth of field of the telecentric lens is within the illumination range of the light source in the specific flow direction.
本发明还提供了一种液体中颗粒物的检测装置,所述检测装置包括图像分析设备以及如上所述的图像采集设备,所述图像分析设备用于接收所述拍摄设备拍摄的颗粒物的图像,并根据所述颗粒物的图像进行分析。The present invention also provides a device for detecting particulate matter in a liquid. The detection device includes an image analysis device and an image acquisition device as described above. The image analysis device is configured to receive an image of the particulate matter captured by the shooting device, and The analysis is based on the image of the particulate matter.
本发明提供的液体中颗粒物的图像采集设备及检测装置,通过设置测样容器使作为样品的液体在其内沿特定流动方向进行流动,并设置光源照射液体中的颗粒物来使颗粒物发生光散射,以光轴方向与所述特定流动方向重合的拍摄设备采集发生光散射的颗粒物的图像,以供图像分析设备进行分析。其有效地解决了颗粒物在流动方向上的速度过快而造成颗粒物的图像出现拖尾模糊的问题,进而使得利用该图像采集设备进行颗粒物的图像采集时,可以不受该条件 限制地提升颗粒物的流动速度,以提高成像通量,提高检测效率,节省检测时间。同时,上述方案利用暗场成像有利于提高获取的颗粒物图像的对比度和信噪比,并且基于对流体中的颗粒物进行成像,有着不受液体中颗粒物种类影响以及可适应外界扰动大的环境的优点。The image acquisition device and detection device for particulate matter in a liquid provided by the present invention, by setting a sample measuring container to make the liquid as a sample flow in a specific flow direction, and setting a light source to irradiate the particulate matter in the liquid to cause light scattering of the particulate matter, A photographing device whose optical axis direction coincides with the specific flow direction collects an image of the light-scattering particulate matter for analysis by the image analysis device. It effectively solves the problem that the speed of the particulate matter in the flow direction is too fast and the image of the particulate matter is smeared, which makes it possible to use the image acquisition device to collect the image of the particulate matter without lifting the conditions. Flow speed to improve imaging flux, improve detection efficiency, and save detection time. At the same time, the above scheme uses dark field imaging to improve the contrast and signal-to-noise ratio of the obtained particulate image, and based on imaging the particulate in the fluid, it has the advantages of not being affected by the type of particulate in the liquid and adapting to the environment with large external disturbances. .
附图说明BRIEF DESCRIPTION
图1是本发明实施例提供的液体中颗粒物的图像采集设备的结构示意图;1 is a schematic structural diagram of an image acquisition device for particulate matter in a liquid provided by an embodiment of the present invention;
图2是所述图像采集设备中一侧的光源对测液容器中的液体进行照射的示例性光路图;2 is an exemplary optical path diagram of a light source on one side of the image acquisition device illuminating the liquid in the liquid measuring container;
图3是所述光源的示例性布局图;3 is an exemplary layout diagram of the light source;
图4是所述废液容器中的子废液容器的示例性连接图。FIG. 4 is an exemplary connection diagram of sub-liquid waste containers in the waste liquid container.
具体实施方式detailed description
为使本发明的目的、技术方案和优点更加清楚,下面结合附图对本发明的具体实施方式进行详细说明。这些优选实施方式的示例在附图中进行了例示。附图中所示和根据附图描述的本发明的实施方式仅仅是示例性的,并且本发明并不限于这些实施方式。To make the objectives, technical solutions, and advantages of the present invention clearer, specific embodiments of the present invention are described in detail below with reference to the drawings. Examples of these preferred embodiments are illustrated in the drawings. The embodiments of the present invention shown in the drawings and described according to the drawings are merely exemplary, and the present invention is not limited to these embodiments.
在此,还需要说明的是,为了避免因不必要的细节而模糊了本发明,在附图中仅仅示出了与根据本发明的方案密切相关的结构和/或处理步骤,而省略了关系不大的其他细节。Here, it should also be noted that, in order to avoid obscuring the present invention due to unnecessary details, only the structures and/or processing steps closely related to the solution according to the present invention are shown in the drawings, and the relationship is omitted. Not much other details.
参阅图1和图2所示,本实施例提供了一种液体中颗粒物的图像采集设备,所述图像采集设备包括测液容器1、光源2以及拍摄设备3。Referring to FIG. 1 and FIG. 2, this embodiment provides an image acquisition device for particles in a liquid. The image acquisition device includes a liquid measuring container 1, a light source 2, and a shooting device 3.
其中,所述测液容器1用于容置具有颗粒物的液体,液体在所述测液容器1内沿特定流动方向进行流动;Wherein, the liquid measuring container 1 is used for containing liquid with particulate matter, and the liquid flows in the liquid measuring container 1 along a specific flow direction;
所述光源2用于对液体进行照射,以使液体内的颗粒物发生光散射;The light source 2 is used to irradiate the liquid to cause light scattering of the particles in the liquid;
所述拍摄设备3的光轴方向与所述特定流动方向重合,所述拍摄设备3用于拍摄液体内发生光散射的颗粒物的图像。The direction of the optical axis of the photographing device 3 coincides with the specific flow direction, and the photographing device 3 is used for photographing an image of particulate matter in which light scattering occurs in the liquid.
本发明实施例提供的一种液体中颗粒物的图像采集设备,利用暗场照明成像的原理,使测液容器1中作为样品的液体沿特定流动方向进行流动的过程中,液体中的颗粒物流经所述光源2的光照范围时发生光散射,并通过拍摄设备3 采集发生光散射的颗粒物的图像,以供相应的计算机等图像分析设备7对图像进行分析,获得液体中颗粒物的形状、尺寸、浓度以及粒径分布等信息。相较于现有技术中明场成像的方案,所述图像采集设备基于暗场成像的原理,不仅能获取颗粒物的纹理以及鞭毛等图像细节信息,还具备有利于提高获取的颗粒物图像的对比度和信噪比的优势。而且重要的是,由于所述图像采集设备的拍摄设备3的光轴方向与所述特定流动方向重合,即颗粒物随液体沿拍摄设备3的光轴方向上进行流动,可以避免拍摄图像出现颗粒物因速度过大而产生颗粒物处的拖尾模糊现象,进而可以不受该因素限制提升颗粒物的流动速度,能使所述图像采集设备的成像通量大幅提升,快速获取大体积液体中所有颗粒物高质量的图像。An image acquisition device for particles in a liquid provided by an embodiment of the present invention uses the principle of dark field illumination imaging to make the liquid in the liquid measuring container 1 flow as a sample in a specific flow direction, and the particles in the liquid flow through Light scattering occurs in the light range of the light source 2, and the image of the light-scattered particles is collected by the shooting device 3, so that the image analysis equipment 7 such as a computer can analyze the image to obtain the shape, size, and Information such as concentration and particle size distribution. Compared with the bright field imaging scheme in the prior art, the image acquisition device is based on the principle of dark field imaging, which can not only obtain the detailed information of the texture of the particulate matter and flagella, but also have the advantages of improving the contrast and granularity of the acquired particulate image. Advantages of signal-to-noise ratio. And importantly, since the direction of the optical axis of the shooting device 3 of the image acquisition device coincides with the specific flow direction, that is, particles flow along the direction of the optical axis of the shooting device 3 with the liquid, it is possible to avoid the occurrence of particles in the captured image. Excessive speed results in smearing of particles, which can be used to increase the flow rate of particles without being restricted by this factor, which can greatly increase the imaging flux of the image acquisition device and quickly obtain high quality of all particles in large volumes of liquid Image.
所述图像采集设备适用于500μm~20mm粒径范围的颗粒物,所述图像采集设备基于流体成像的原理还具有不受液体中颗粒物种类影响的优点,其不仅能对没有游动能力的悬浮颗粒进行成像,还能对游动能力较强的活体浮游生物进行成像,另外,其无需等待颗粒物沉降至测液容器的底部来采集图像,外界干扰对所述图像采集设备的正常工作的影响较小,使所述图像采集设备能适用于浮排、科考船等现场环境。The image acquisition device is suitable for particles with a size range of 500 μm to 20 mm. The image acquisition device is based on the principle of fluid imaging and has the advantage of not being affected by the type of particles in the liquid. It can not only perform suspended particles without swimming ability Imaging can also image living plankton with strong swimming ability. In addition, it does not need to wait for the particulate matter to settle to the bottom of the liquid measurement container to collect images. The external interference has little effect on the normal operation of the image collection device. The image acquisition device can be applied to on-site environments such as floating row and scientific research ship.
具体地,所述测液容器1包括暂存管12和设置于所述暂存管12内的进样管11,所述进样管11的底端与所述暂存管12的底端之间具有预定距离,所述进样管11的底端开放,以与所述暂存管12连通;其中,液体经由所述进样管11的底端流入到所述暂存管12。利用所述测液容器1中的暂存管12套设于进样管11上的结构,使得液体从所述进样管11的底端流出后,能在暂存管12的底端的阻挡下,从所述暂存管12的底端向四周流动,已经过图像采集的液体快速地远离所述拍摄设备3的视场,避免了其对拍摄图像造成影响。上述进样管11的底端与所述暂存管12的底端之间的预定距离需要根据所拍摄颗粒物的最大粒径进行决定,若所述预定距离过大,则所拍摄颗粒物通过进样管11后难以在较短时间内远离拍摄设备3的视场,增加了焦面目标发出的光被预定距离内的颗粒物遮挡几率;若所述预定距离小于最大的颗粒物的粒径,则所拍摄颗粒物可能无法从所述进样管11的底端流出,造成堵塞,遮挡成像,且还会对较为柔弱的颗粒物形态造成损伤。优选将所述预定距离设置为等于最大的颗粒物的粒径。另外,为使所述光源2能透过所述测液容器1对液体进行照射,所述进样管11和暂存管12为高透明的容器。Specifically, the liquid measuring container 1 includes a temporary storage tube 12 and a sampling tube 11 disposed in the temporary storage tube 12, the bottom end of the sampling tube 11 and the bottom end of the temporary storage tube 12 There is a predetermined distance between them, and the bottom end of the sampling tube 11 is open to communicate with the temporary storage tube 12; wherein, the liquid flows into the temporary storage tube 12 via the bottom end of the sampling tube 11. With the structure that the temporary storage tube 12 in the liquid measuring container 1 is sleeved on the sampling tube 11, the liquid can flow out from the bottom end of the sampling tube 11 under the obstruction of the bottom end of the temporary storage tube 12 From the bottom end of the temporary storage tube 12 to the surroundings, the liquid that has undergone image acquisition quickly moves away from the field of view of the shooting device 3, avoiding it from affecting the captured image. The predetermined distance between the bottom end of the sampling tube 11 and the bottom end of the temporary storage tube 12 needs to be determined according to the maximum particle size of the captured particulate matter. If the predetermined distance is too large, the captured particulate matter passes through the sample After the tube 11, it is difficult to stay away from the field of view of the shooting device 3 in a short period of time, increasing the probability that the light emitted by the focal plane target is blocked by particles within a predetermined distance; if the predetermined distance is less than the particle size of the largest particle, the shot Particulate matter may not flow out from the bottom end of the sampling tube 11, causing blockage, blocking imaging, and also damaging the form of relatively weak particulate matter. Preferably, the predetermined distance is set equal to the particle size of the largest particulate matter. In addition, in order to allow the light source 2 to irradiate the liquid through the liquid measuring container 1, the sampling tube 11 and the temporary storage tube 12 are highly transparent containers.
如图2所示,在本实施例中,所述光源2选用发散角较小的光源,所述光 源的光线中心面20与所述拍摄设备3的物方焦平面重合。例如,如图2中所示,光线中心面20位于光照范围的边缘两侧的示例性光路a和b的中心,而所述拍摄设备3的物方焦平面与所述光线中心面20重合,所述拍摄设备3的景深位于所述光源2的光照范围匹配,其能确保处于所述拍摄设备3景深内的颗粒都处于所述光源2的照明范围内,从而能拍摄到清晰的颗粒物图像。进一步地,通过使所述光源2产生的光的光路垂直于所述拍摄设备3的光轴,避免所述光源2产生的光线进入到所述拍摄设备3中,影响拍摄图像。As shown in FIG. 2, in this embodiment, the light source 2 uses a light source with a small divergence angle, and the light center plane 20 of the light source coincides with the object-side focal plane of the shooting device 3. For example, as shown in FIG. 2, the light center plane 20 is located at the center of the exemplary light paths a and b on both sides of the edge of the illumination range, and the object-side focal plane of the shooting device 3 coincides with the light center plane 20, The depth of field of the shooting device 3 is matched with the illumination range of the light source 2, which can ensure that the particles within the depth of field of the shooting device 3 are all within the illumination range of the light source 2, so that a clear image of particles can be captured. Further, by making the optical path of the light generated by the light source 2 perpendicular to the optical axis of the shooting device 3, the light generated by the light source 2 is prevented from entering the shooting device 3 and affecting the captured image.
示例性地,结合图3所示,所述光源2包括两个以上并以相互之间保持相等的夹角环绕所述测液容器1进行设置的照明器21,本实施例选用三个条形的照明器21,通过嵌入设置的光源支架中,使所述三个照明器21环绕所述测液容器1,进而使所述三个照明器21的光照范围完全覆盖所述测液容器1中测试区域的横截面,例如,如图3中所示,若所述测液容器1的进样管11选用圆柱形的管状容器,则所述三个照明器21产生光照范围的交叠区域的面积大于所述进样管11的横截面的面积,所述进样管11边缘上所有位置均处于光照范围内。而为了减少颗粒径向移动产生的运动模糊,所述拍摄设备3需要进行短时间的曝光,故所述照明器21需选用高光强的照明器。另外,所述光源2可选用单色光源或由对应三原色的至少三个照明器21构成的彩色光源,分别用于获取颗粒物的灰度图像或彩色图像。Exemplarily, referring to FIG. 3, the light source 2 includes more than two illuminators 21 that are arranged around the liquid measuring container 1 at an equal angle with each other. In this embodiment, three strips are selected. The illuminator 21 is embedded in the light source bracket, so that the three illuminators 21 surround the liquid measuring container 1, so that the illumination range of the three illuminators 21 completely covers the liquid measuring container 1 The cross-section of the test area, for example, as shown in FIG. 3, if the sampling tube 11 of the liquid measuring container 1 selects a cylindrical tubular container, the three illuminators 21 produce overlapping areas of the illumination range The area is larger than the cross-sectional area of the sampling tube 11, and all positions on the edge of the sampling tube 11 are within the illumination range. In order to reduce the motion blur caused by the radial movement of the particles, the shooting device 3 needs to be exposed for a short time, so the illuminator 21 needs to use a high-intensity illuminator. In addition, the light source 2 may be a monochromatic light source or a color light source composed of at least three illuminators 21 corresponding to the three primary colors, which are respectively used to obtain a grayscale image or a color image of the particulate matter.
进一步地,所述光源2所发出光束的底端所在平面与所述进样管11的底端所在平面重合。防止未被照明的颗粒物对成像造成遮挡的现象发生,提高图像的信噪比和对比度。Further, the plane of the bottom end of the light beam emitted by the light source 2 coincides with the plane of the bottom end of the sampling tube 11. Prevent the phenomenon that unilluminated particles block the imaging, and improve the signal-to-noise ratio and contrast of the image.
具体地,所述拍摄设备3包括远心镜头31,所述远心镜头31的景深与所述光源2在所述特定流动方向上的光照范围匹配,优选地,所述远心镜头31的景深与所述光源2在所述特定流动方向上的光照范围重合。远心镜头31能使在景深范围内获取的图像的放大倍率相同,当景深与光照范围匹配时,即使液体中发生光散射的颗粒物处于所述进样管11内的不同高度处,所述拍摄设备3通过所述远心镜头31拍摄的图像中,所有颗粒物的成像的放大倍率一致,不会因距离所述远心镜头31的远近不同而产生偏差,从而避免了上述原因导致分析获得的颗粒物的尺寸信息出现误差。例如,当所述光源2设置为上述由三个条形的照明器构成的光源时,用于限定所述远心镜头31的景深范围的两个调焦平面分别与光照范围的边缘两侧的光路a和b相重合。Specifically, the shooting device 3 includes a telecentric lens 31 whose depth of field matches the illumination range of the light source 2 in the specific flow direction, preferably, the depth of field of the telecentric lens 31 It coincides with the light range of the light source 2 in the specific flow direction. The telecentric lens 31 can make the magnification of the image acquired in the depth of field range the same. When the depth of field matches the illumination range, even if the light scattering particles in the liquid are at different heights in the sampling tube 11, the shooting In the image taken by the device 3 through the telecentric lens 31, the imaging magnification of all particles is the same, and there will be no deviation due to the difference in distance from the telecentric lens 31, thereby avoiding the particles caused by the above reasons. Has an error in the size information. For example, when the light source 2 is configured as the above-mentioned light source composed of three strip-shaped illuminators, the two focusing planes used to define the depth of field range of the telecentric lens 31 are respectively The optical paths a and b coincide.
进一步地,所述图像采集设备还包括与所述测液容器1连通的供液容器4,所述供液容器4用于向所述测液容器1提供具有颗粒物的液体。所述供液容器4与所述测液容器1的进样管11的顶端连通,具体地,所述供液容器4为容积远大于所述进样管11的容器,使得能提供充足量的液体以进行高通量成像。Further, the image acquisition device further includes a liquid supply container 4 communicating with the liquid measuring container 1, and the liquid supply container 4 is used to supply the liquid with particulate matter to the liquid measuring container 1. The liquid supply container 4 is in communication with the top of the sampling tube 11 of the liquid measuring container 1. Specifically, the liquid supply container 4 is a container whose volume is much larger than that of the sampling tube 11, so that a sufficient amount of Liquid for high-throughput imaging.
更进一步地,所述图像采集设备还包括与所述供液容器4的底端连通的压力泵51以及与所述压力泵51连通的增液容器5,所述压力泵51用于将所述增液容器5内的液体注射至所述供液容器4内。所述压力泵51通过将所述供液容器4中液体从所述供液容器4的底端注入,一方面使得所述供液容器4底部的液体发生流动,防止所述供液容器4中的颗粒物沉降于其底部,避免由于颗粒物集中进入所述进样管11而对成像造成影响,进而避免影响检测结果的准确度;另一方面,通过为所述供液容器4补充液体,可以控制所述供液容器4中的液位高度,进而控制液体在所述进样管11中流动的速度。所述液体可以选用纯水、纯净的海水以及作为样品的液体等不会对颗粒物造成干扰的液体。当然,作为另一种实施方式,若只考虑用于防止所述供液容器4中的颗粒物沉降于底部,也可以只通过所述压力泵51向所述供液容器4的底部注入气体来扰动所述供液容器4底部的液体,从而起到上述避免由于颗粒物集中进入所述进样管11而对成像造成影响的作用。作为又一种实施方式,所述图像采集设备还包括浸没在所述供液容器4的底部的旋转叶片以及与所述旋转叶片电性连接的电机,所述电机用于驱动所述旋转叶片在所述供液容器4中进行旋转,以起到扰动所述供液容器4底部的液体的作用。Furthermore, the image acquisition device further includes a pressure pump 51 communicating with the bottom end of the liquid supply container 4 and a liquid increasing container 5 communicating with the pressure pump 51, and the pressure pump 51 is used to The liquid in the liquid increasing container 5 is injected into the liquid supply container 4. The pressure pump 51 injects the liquid in the liquid supply container 4 from the bottom end of the liquid supply container 4, on the one hand, the liquid at the bottom of the liquid supply container 4 flows to prevent the liquid supply container 4 from The particulate matter settles on the bottom of it, avoiding the concentration of particulate matter entering the sample tube 11 and affecting the imaging, thereby avoiding affecting the accuracy of the detection results; on the other hand, by supplementing the liquid supply container 4 with liquid, it can be controlled The liquid level in the liquid supply container 4 further controls the speed of the liquid flowing in the sampling tube 11. The liquid can be selected from pure water, pure sea water, and liquid as a sample, which does not interfere with particulate matter. Of course, as another embodiment, if it is only considered to prevent the particulate matter in the liquid supply container 4 from sinking to the bottom, it may also be disturbed only by injecting gas into the bottom of the liquid supply container 4 through the pressure pump 51 The liquid at the bottom of the liquid supply container 4 serves to avoid the influence of imaging on the sample tube 11 due to the concentration of the particulate matter. As yet another embodiment, the image acquisition device further includes a rotary blade immersed in the bottom of the liquid supply container 4 and a motor electrically connected to the rotary blade, the motor is used to drive the rotary blade in The liquid supply container 4 rotates to disturb the liquid at the bottom of the liquid supply container 4.
进一步地,所述图像采集设备还包括:负压泵61和与所述测液容器1的底端连通的废液容器6,所述废液容器6为密闭的容器,其密封性良好,所述负压泵61与所述废液容器6连通,以调节所述废液容器6内的气压,利用所述废液容器6的气压可以间接地调控所述测液容器1内的液体的流动速度,避免液体中的易损的颗粒物发生损坏,例如海雪等样品。所述废液容器6中采集的液体可用于回收利用,避免样品的浪费。Further, the image acquisition device further includes: a negative pressure pump 61 and a waste liquid container 6 communicating with the bottom end of the liquid measuring container 1. The waste liquid container 6 is a closed container, which has a good sealability, so The negative pressure pump 61 communicates with the waste liquid container 6 to adjust the air pressure in the waste liquid container 6, and the air pressure in the waste liquid container 6 can be used to indirectly regulate the flow of liquid in the liquid measurement container 1 Speed, to avoid damage to vulnerable particles in the liquid, such as sea snow and other samples. The liquid collected in the waste liquid container 6 can be used for recycling to avoid the waste of samples.
为确保在同次检测中,能利用所述负压泵61调控所述测液容器1内的液体的流动速度,所述废液容器6的容积需要足够大。示例性地,参阅图4所示,所述废液容器6包括多个子废液容器60,所述多个子废液容器60依照一定的规律彼此连通,例如通过依次串联的方式或者以一个子废液容器60并联多个子废液容器60等等方式,以增加连通的子废液容器60的数目的方法来增大所述废液容器6的容积。In order to ensure that the negative pressure pump 61 can be used to regulate the flow rate of the liquid in the liquid measuring container 1 during the same inspection, the volume of the waste liquid container 6 needs to be large enough. Exemplarily, as shown in FIG. 4, the waste liquid container 6 includes a plurality of sub-waste liquid containers 60, and the plurality of sub-waste liquid containers 60 are connected to each other according to a certain rule, for example, by serial connection in series or by one sub-waste The liquid container 60 is connected in parallel with a plurality of child waste liquid containers 60 and the like to increase the volume of the waste liquid container 6 by increasing the number of the connected child waste liquid containers 60.
所述液体中颗粒物的图像采集设备还包括支架8,分别将测液容器1、光源2、拍摄设备3以及供液容器4固定在所述支架8的对应位置上,使得所述测液容器1的延伸方向与重力方向一致,液体能在重力的作用下自然地沿所述特定流动方向进行流动。所述支架8选用重量轻且强度高的铝材支架,支架8上的各个装配位置可调。当然,作为其他的实施方式,也可以通过驱动装置驱动液体沿所述特定流动方向进行流动。The image acquisition device for the particulate matter in the liquid further includes a bracket 8 to fix the liquid measurement container 1, the light source 2, the photographing device 3, and the liquid supply container 4 at corresponding positions of the bracket 8 so that the liquid measurement container 1 The extension direction of is consistent with the direction of gravity, and the liquid can naturally flow along the specific flow direction under the action of gravity. The bracket 8 is selected from a light-weight and high-strength aluminum bracket, and each assembly position on the bracket 8 is adjustable. Of course, as other embodiments, the driving device may also drive the liquid to flow in the specific flow direction.
基于上述液体中颗粒物的图像采集设备的示例性使用过程如下:首先通过供液容器4向所述测样容器1中注入纯净的海水,直至海水没过所述测样容器1的进样管11中对应所述拍摄设备3的景深的深度;再通过供液容器4向所述测样容器1的进样管11中注入作为待测样品的液体,使所述拍摄设备3进行拍摄并通过控制泵阀使液体进行流动,待作为待测样品的液体完全从所述测样容器1中流出后,再次向所述测样容器1中注入纯净的海水,直至所述拍摄设备3拍摄的图像中不再出现颗粒物,停止拍摄并关闭泵阀,完成图像采集。An exemplary use process of the image acquisition device based on the particulate matter in the liquid is as follows: First, pure sea water is injected into the sample container 1 through the liquid supply container 4 until the sea water does not pass through the sampling tube 11 of the sample container 1 Corresponds to the depth of field of the shooting device 3; then, the liquid supply container 4 injects the liquid as the sample to be tested into the sampling tube 11 of the sample container 1, so that the shooting device 3 performs shooting and passes the control The pump valve causes the liquid to flow. After the liquid as the sample to be tested completely flows out of the sample container 1, pure sea water is injected into the sample container 1 again until the image captured by the shooting device 3 No more particles appear, stop shooting and close the pump valve to complete the image acquisition.
本发明还提供了一种液体中颗粒物的检测装置,所述检测装置包括图像分析设备7以及如上所述的图像采集设备,所述图像分析设备7用于接收所述拍摄设备3拍摄的颗粒物的图像,并根据所述颗粒物的图像进行分析。The present invention also provides a device for detecting particulate matter in a liquid. The detection device includes an image analysis device 7 and an image acquisition device as described above. The image analysis device 7 is configured to receive particles captured by the shooting device 3. Image, and analyze based on the image of the particulate matter.
上述液体中颗粒物的检测装置中的部件可划分为以下模块:成像单元、进样单元、扰动单元、流体控制单元以及图像分析单元,其中,所述成像单元由所述光源2和所述拍摄设备3构成,所述进样单元由所述测液容器1和所述供液容器4构成,所述扰动单元由所述压力泵51及所述增液容器5构成,所述流体控制单元由所述负压泵61、所述废液容器6、所述供液容器4、所述压力泵51及所述增液容器5构成,所述图像分析单元由所述图像分析设备构成。The components in the above-mentioned device for detecting particulate matter in a liquid can be divided into the following modules: an imaging unit, a sampling unit, a disturbance unit, a fluid control unit, and an image analysis unit, wherein the imaging unit is composed of the light source 2 and the shooting device 3, the sampling unit is composed of the liquid measuring container 1 and the liquid supply container 4, the disturbance unit is composed of the pressure pump 51 and the liquid increase container 5, the fluid control unit is composed of The negative pressure pump 61, the waste liquid container 6, the liquid supply container 4, the pressure pump 51, and the booster container 5 are configured, and the image analysis unit is configured by the image analysis device.
上述液体中颗粒物的检测装置中,所述拍摄设备3的帧率和所述进样管11内液体的流速遵循以下参数之间的关系式:In the above device for detecting particulate matter in liquid, the frame rate of the shooting device 3 and the flow rate of the liquid in the sampling tube 11 follow the relationship between the following parameters:
f=v/(1*s)f=v/(1*s)
其中,f为所述拍摄设备3的帧率,v为所述进样管11内液体的流量,1为所述拍摄设备3的景深,s为所述进样管11的横截面积,即所述拍摄设备3的帧率等于所述进样管11内液体的流量除以所述拍摄设备3的景深与所述进样管11的横截面积的乘积。由此,当选定所述进样管11以及所述拍摄设备3的景深后,通过调控所述拍摄设备3的帧率f和所述进样管11内液体的流量v来确保测量数据的准确性,并且,可根据该公式,当设定好所述拍摄设备3的帧率f 和所述进样管11内液体的流量v中的一方的参数后,可依照上述关系式反馈另一方的参数。Where f is the frame rate of the shooting device 3, v is the flow rate of the liquid in the sampling tube 11, 1 is the depth of field of the shooting device 3, and s is the cross-sectional area of the sampling tube 11, ie The frame rate of the shooting device 3 is equal to the flow rate of the liquid in the sampling tube 11 divided by the product of the depth of field of the shooting device 3 and the cross-sectional area of the sampling tube 11. Therefore, when the depth of field of the sampling tube 11 and the shooting device 3 is selected, the frame rate f of the shooting device 3 and the flow rate v of the liquid in the sampling tube 11 are adjusted to ensure the measurement data Accuracy, and according to this formula, after setting the parameters of one of the frame rate f of the shooting device 3 and the flow rate v of the liquid in the sampling tube 11, the other party can be fed back according to the above relationship Parameters.
综上所述,本发明提供的液体中颗粒物的图像采集设备及检测装置,通过设置测样容器1使作为样品的液体在其内沿特定流动方向进行流动,并设置光源2照射液体中的颗粒物来使颗粒物发生光散射,以光轴方向与所述特定流动方向重合的拍摄设备3采集发生光散射的颗粒物的图像,以供图像分析设备7进行分析。其有效地解决了颗粒物在流动方向上的速度过快而造成颗粒物的图像出现拖尾模糊的问题,进而使得利用该图像采集设备进行颗粒物的图像采集时,可以不受该条件限制地提升颗粒物的流动速度,以提高成像通量,提高检测效率,节省检测时间。同时,上述方案利用暗场成像有利于提高获取的颗粒物图像的对比度和信噪比,并且基于对流体中的颗粒物进行成像,有着不受液体中颗粒物种类影响以及可适应外界扰动大的环境的优点。In summary, the image acquisition device and detection device for particulate matter in liquid provided by the present invention, by providing a sample measuring container 1 to allow the liquid as a sample to flow in a specific flow direction, and a light source 2 to irradiate particulate matter in the liquid To cause light scattering of the particulate matter, the imaging device 3 whose optical axis direction coincides with the specific flow direction collects an image of the light scattering particulate matter for analysis by the image analysis device 7. It effectively solves the problem that the speed of the particulate matter in the flow direction is too fast and the image of the particulate matter is smeared, which makes it possible to use the image acquisition device to collect the image of the particulate matter without lifting the conditions. Flow speed to improve imaging flux, improve detection efficiency, and save detection time. At the same time, the above scheme uses dark field imaging to improve the contrast and signal-to-noise ratio of the obtained particulate image, and based on imaging the particulate in the fluid, it has the advantages of not being affected by the type of particulate in the liquid and adapting to the environment with large external disturbances. .
需要说明的是,在本文中,诸如第一和第二等之类的关系术语仅仅用来将一个实体或者操作与另一个实体或操作区分开来,而不一定要求或者暗示这些实体或操作之间存在任何这种实际的关系或者顺序。而且,术语“包括”、“包含”或者其任何其他变体意在涵盖非排他性的包含,从而使得包括一系列要素的过程、方法、物品或者设备不仅包括那些要素,而且还包括没有明确列出的其他要素,或者是还包括为这种过程、方法、物品或者设备所固有的要素。在没有更多限制的情况下,由语句“包括一个……”限定的要素,并不排除在包括所述要素的过程、方法、物品或者设备中还存在另外的相同要素。It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that these entities or operations There is any such actual relationship or order. Moreover, the terms "include", "include" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device that includes a series of elements includes not only those elements, but also those not explicitly listed Or other elements that are inherent to this process, method, article, or equipment. Without more restrictions, the element defined by the sentence "include one..." does not exclude that there are other identical elements in the process, method, article or equipment that includes the element.
以上所述仅是本申请的具体实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本申请原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本申请的保护范围。The above is only the specific implementation of this application. It should be pointed out that for those of ordinary skill in the art, without departing from the principles of this application, several improvements and retouches can be made. These improvements and retouches also It should be regarded as the scope of protection of this application.

Claims (19)

  1. 一种液体中颗粒物的图像采集设备,其中,所述图像采集设备包括:An image acquisition device for particles in a liquid, wherein the image acquisition device includes:
    测液容器(1),用于容置具有颗粒物的液体,液体在所述测液容器(1)内沿特定流动方向进行流动;A liquid measuring container (1) for containing liquid with particulate matter, and the liquid flows in a specific flow direction in the liquid measuring container (1);
    光源(2),用于对液体进行照射,以使液体内的颗粒物发生光散射;The light source (2) is used to irradiate the liquid to cause light scattering of the particles in the liquid;
    拍摄设备(3),所述拍摄设备(3)的光轴方向与所述特定流动方向重合,所述拍摄设备(3)用于拍摄液体内发生光散射的颗粒物的图像。A photographing device (3), the direction of the optical axis of the photographing device (3) coincides with the specific flow direction, and the photographing device (3) is used to photograph an image of a particulate matter in which light scattering occurs in the liquid.
  2. 根据权利要求1所述的图像采集设备,其中,所述光源(2)的光线中心面与所述拍摄设备(3)的物方焦平面重合。The image acquisition device according to claim 1, wherein the light center plane of the light source (2) coincides with the object-side focal plane of the shooting device (3).
  3. 根据权利要求2所述的图像采集设备,其中,所述光源(2)所发出光束的底端所在平面与所述进样管(11)的底端所在平面重合。The image acquisition device according to claim 2, wherein the plane of the bottom end of the light beam emitted by the light source (2) coincides with the plane of the bottom end of the sampling tube (11).
  4. 根据权利要求1所述的图像采集设备,其中,所述测液容器(1)包括暂存管(12)和设置于所述暂存管(12)内的进样管(11),所述进样管(11)的底端与所述暂存管(12)的底端之间具有预定距离,所述进样管(11)的底端开放,以与所述暂存管(12)连通;其中,液体经由所述进样管(11)的底端流入到所述暂存管(12)。The image acquisition device according to claim 1, wherein the liquid measuring container (1) includes a temporary storage tube (12) and a sampling tube (11) provided in the temporary storage tube (12), the There is a predetermined distance between the bottom end of the sampling tube (11) and the bottom end of the temporary storage tube (12), and the bottom end of the sampling tube (11) is open to communicate with the temporary storage tube (12) Communication; wherein, the liquid flows into the temporary storage tube (12) via the bottom end of the sampling tube (11).
  5. 根据权利要求4所述的图像采集设备,其中,所述光源(2)的光线中心面与所述拍摄设备(3)的物方焦平面重合。The image acquisition device according to claim 4, wherein the center plane of light rays of the light source (2) coincides with the object-side focal plane of the shooting device (3).
  6. 根据权利要求1所述的图像采集设备,其中,所述拍摄设备(3)包括远心镜头(31),所述远心镜头(31)的景深与所述光源(2)在所述特定流动方向上的光照范围重合。The image acquisition device according to claim 1, wherein the photographing device (3) includes a telecentric lens (31), the depth of field of the telecentric lens (31) and the light source (2) flow in the specific flow The light range in the direction coincides.
  7. 根据权利要求1所述的图像采集设备,其中,所述图像采集设备还包括与所述测液容器(1)连通的供液容器(4),所述供液容器(4)用于向所述测液容器(1)提供具有颗粒物的液体。The image acquisition device according to claim 1, wherein the image acquisition device further comprises a liquid supply container (4) in communication with the liquid measurement container (1), the liquid supply container (4) is used to The liquid measuring container (1) provides liquid with particulate matter.
  8. 根据权利要求7所述的图像采集设备,其中,所述图像采集设备还包括与所述供液容器(4)的底端连通的压力泵(51)以及与所述压力泵(51)连通的增液容器(5),所述压力泵(51)用于将所述增液容器(5)内的液体注射至 所述供液容器(4)内。The image acquisition device according to claim 7, wherein the image acquisition device further includes a pressure pump (51) communicating with the bottom end of the liquid supply container (4) and a pressure pump (51) communicating with the pressure pump (51) A liquid increasing container (5), the pressure pump (51) is used to inject the liquid in the liquid increasing container (5) into the liquid supply container (4).
  9. 根据权利要求1所述的图像采集设备,其中,所述图像采集设备还包括:负压泵(61)和与所述测液容器(1)的底端连通的废液容器(6),所述负压泵(61)与所述废液容器(6)连通,以调节所述废液容器(6)内的气压。The image acquisition device according to claim 1, wherein the image acquisition device further comprises: a negative pressure pump (61) and a waste liquid container (6) communicating with the bottom end of the liquid measurement container (1), so The negative pressure pump (61) communicates with the waste liquid container (6) to adjust the air pressure in the waste liquid container (6).
  10. 根据权利要求9所述的图像采集设备,其中,所述废液容器(6)包括多个子废液容器(60),所述多个子废液容器(60)彼此连通。The image acquisition device according to claim 9, wherein the waste liquid container (6) includes a plurality of sub waste liquid containers (60), and the plurality of sub waste liquid containers (60) communicate with each other.
  11. 一种液体中颗粒物的检测装置,其中,所述检测装置包括图像分析设备(7)以及图像采集设备,所述图像采集设备包括测液容器(1)、光源(2)以及拍摄设备(3),A detection device for particulate matter in a liquid, wherein the detection device includes an image analysis device (7) and an image acquisition device, the image acquisition device includes a liquid measurement container (1), a light source (2), and a shooting device (3) ,
    所述测液容器(1)用于容置具有颗粒物的液体,液体在所述测液容器(1)内沿特定流动方向进行流动;The liquid measuring container (1) is used for containing liquid with particulate matter, and the liquid flows in a specific flow direction in the liquid measuring container (1);
    所述光源(2)用于对液体进行照射,以使液体内的颗粒物发生光散射;The light source (2) is used to irradiate the liquid to cause light scattering of the particles in the liquid;
    所述拍摄设备(3)的光轴方向与所述特定流动方向重合,所述拍摄设备(3)用于拍摄液体内发生光散射的颗粒物的图像,The direction of the optical axis of the photographing device (3) coincides with the specific flow direction, and the photographing device (3) is used to photograph an image of particulate matter in which light scattering occurs in the liquid,
    其中,所述图像分析设备(7)用于接收所述拍摄设备(3)拍摄的颗粒物的图像,并根据所述颗粒物的图像进行分析。Wherein, the image analysis device (7) is used to receive the image of the particulate matter captured by the photographing device (3), and analyze based on the image of the particulate matter.
  12. 根据权利要求11所述的检测装置,其中,所述光源(2)的光线中心面与所述拍摄设备(3)的物方焦平面重合。The detection device according to claim 11, wherein the light center plane of the light source (2) coincides with the object-side focal plane of the photographing device (3).
  13. 根据权利要求12所述的检测装置,其中,所述光源(2)所发出光束的底端所在平面与所述进样管(11)的底端所在平面重合。The detection device according to claim 12, wherein the plane of the bottom end of the light beam emitted by the light source (2) coincides with the plane of the bottom end of the sampling tube (11).
  14. 根据权利要求11所述的检测装置,其中,所述测液容器(1)包括暂存管(12)和设置于所述暂存管(12)内的进样管(11),所述进样管(11)的底端与所述暂存管(12)的底端之间具有预定距离,所述进样管(11)的底端开放,以与所述暂存管(12)连通;其中,液体经由所述进样管(11)的底端流入到所述暂存管(12)。The detection device according to claim 11, wherein the liquid measuring container (1) includes a temporary storage tube (12) and a sampling tube (11) provided in the temporary storage tube (12), the There is a predetermined distance between the bottom end of the sample tube (11) and the bottom end of the temporary storage tube (12), and the bottom end of the sampling tube (11) is open to communicate with the temporary storage tube (12) ; Wherein, the liquid flows into the temporary storage tube (12) via the bottom end of the sampling tube (11).
  15. 根据权利要求11所述的检测装置,其中,所述拍摄设备(3)包括远心镜头(31),所述远心镜头(31)的景深与所述光源(2)在所述特定流动方向上的光照范围重合。The detection device according to claim 11, wherein the photographing device (3) includes a telecentric lens (31), the depth of field of the telecentric lens (31) and the light source (2) in the specific flow direction The light ranges on the above coincide.
  16. 根据权利要求11所述的检测装置,其中,所述图像采集设备还包括与所述测液容器(1)连通的供液容器(4),所述供液容器(4)用于向所述测液容器(1)提供具有颗粒物的液体。The detection device according to claim 11, wherein the image acquisition device further comprises a liquid supply container (4) in communication with the liquid measurement container (1), the liquid supply container (4) is used to The liquid measuring container (1) provides liquid with particulate matter.
  17. 根据权利要求16所述的检测装置,其中,所述图像采集设备还包括与所述供液容器(4)的底端连通的压力泵(51)以及与所述压力泵(51)连通的增液容器(5),所述压力泵(51)用于将所述增液容器(5)内的液体注射至所述供液容器(4)内。The detection device according to claim 16, wherein the image acquisition device further comprises a pressure pump (51) in communication with the bottom end of the liquid supply container (4) and an increasing pressure in communication with the pressure pump (51) A liquid container (5), and the pressure pump (51) is used to inject the liquid in the liquid increasing container (5) into the liquid supply container (4).
  18. 根据权利要求11所述的检测装置,其中,所述图像采集设备还包括:负压泵(61)和与所述测液容器(1)的底端连通的废液容器(6),所述负压泵(61)与所述废液容器(6)连通,以调节所述废液容器(6)内的气压。The detection device according to claim 11, wherein the image acquisition device further comprises: a negative pressure pump (61) and a waste liquid container (6) communicating with the bottom end of the liquid measurement container (1), the A negative pressure pump (61) communicates with the waste liquid container (6) to adjust the air pressure in the waste liquid container (6).
  19. 根据权利要求18所述的检测装置,其中,所述废液容器(6)包括多个子废液容器(60),所述多个子废液容器(60)彼此连通。The detection device according to claim 18, wherein the waste liquid container (6) includes a plurality of sub waste liquid containers (60), and the plurality of sub waste liquid containers (60) communicate with each other.
PCT/CN2019/123649 2018-12-14 2019-12-06 Image acquisition device and detection apparatus for particulate matter in liquid WO2020119600A1 (en)

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