WO2020105649A1 - Procédé de production de légumes à feuilles - Google Patents

Procédé de production de légumes à feuilles

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Publication number
WO2020105649A1
WO2020105649A1 PCT/JP2019/045327 JP2019045327W WO2020105649A1 WO 2020105649 A1 WO2020105649 A1 WO 2020105649A1 JP 2019045327 W JP2019045327 W JP 2019045327W WO 2020105649 A1 WO2020105649 A1 WO 2020105649A1
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WO
WIPO (PCT)
Prior art keywords
day
oxidized glutathione
germination
leafy vegetables
period
Prior art date
Application number
PCT/JP2019/045327
Other languages
English (en)
Japanese (ja)
Inventor
小川　健一
靖史 大門
季之 高橋
功英 石橋
Original Assignee
岡山県
株式会社アースサイド
Ｊｘｔｇエネルギー株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 岡山県, 株式会社アースサイド, Ｊｘｔｇエネルギー株式会社 filed Critical 岡山県
Publication of WO2020105649A1 publication Critical patent/WO2020105649A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides

Definitions

  • the present invention relates to a method for producing leafy vegetables.
  • Glutathione is a peptide consisting of three amino acids, L-cysteine, L-glutamic acid, and glycine, which exists not only in the human body, but in many living organisms such as other animals, plants, and microorganisms, and removes active oxygen. Functions such as action and detoxification of peroxide are known.
  • Glutathione is composed of reduced glutathione, which is present as a single peptide molecule consisting of three amino acids, L-cysteine, L-glutamic acid, and glycine, and oxidized glutathione, in which two molecules of reduced glutathione are bound via a disulfide bond. It exists in either form.
  • Oxidized glutathione is known to be useful in the fertilizer field because it has a function of promoting plant growth. For example, it has been reported that the number of seeds or flowers of a plant is increased by administering a plant with oxidized glutathione (Patent Document 1). Further, it has been reported that the growth of tubers and the like was promoted by administering oxidized glutathione to tubers and the like in the bud stage (Patent Document 2). Furthermore, it has been reported that changes in growth and zinc content were observed when oxidized glutathione was administered to leaf lettuce (Patent Document 3).
  • the present invention provides a method for producing leafy vegetables having excellent production efficiency.
  • leaf vegetables are produced, which comprises a step of applying oxidized glutathione to the underground part of leaf vegetables at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a method is provided.
  • the step of applying oxidized glutathione to the underground portion of the leaf vegetables is used.
  • a method of increasing yield is provided.
  • a composition for increasing the yield of leafy vegetables comprising oxidized glutathione, at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a composition for applying oxidized glutathione to the underground part of leafy vegetables comprising oxidized glutathione, at least within the seedling raising period from the day of germination to the 12th day after germination.
  • the present invention it is advantageous in that the weight of leaf vegetables produced is increased. Further, according to the present invention, it is possible to reduce the number of production (cultivation) days required when the final weight of leaf vegetables produced is the same.
  • oxidized glutathione means reduced glutathione (N- (N- ⁇ -L-glutamyl-L-cysteinyl) glycine). It is a compound in which two molecules are bound via a disulfide bond.
  • oxidized glutathione is not bound to other substances and is not an ionized educt, a salt formed with oxidized glutathione and an acid or a base, a hydrate of these, Various forms such as a mixture may be included.
  • the above-mentioned oxidized glutathione salt is not particularly limited as long as it is a salt acceptable as a fertilizer such as ammonium salt, calcium salt, magnesium salt, sodium salt, lithium salt and the like.
  • the salt of oxidized glutathione is preferably one or more salts selected from ammonium salts, calcium salts and magnesium salts.
  • the leafy vegetables produced by the method of the present invention may be of any type as long as they are vegetables whose leaf portion is mainly edible.
  • leafy vegetables produced in the present invention include ruffle lettuce, romaine lettuce, batavia lettuce, lorobionda, lolorossa, butterhead lettuce, leaf lettuce such as oakley lettuce; headlet lettuce; mustard vegetable (wasabi vegetable), Mizuna , Leaf radish, Chinese cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage and other cruciferous leafy vegetables, chard (Swiss chard), spinach (mainly for salad variety), or other such as chrysanthemum Examples include leafy vegetables.
  • leaf lettuce such as frill lettuce, romaine lettuce, Batavia lettuce, lorobinonda, lolorossa, butterhead lettuce, or oakley lettuce is preferable.
  • the seeds of leafy vegetables or seedlings immediately after germination used for the production of leafy vegetables can be obtained by methods known to those skilled in the art.
  • the “underground part of leafy vegetables” refers to a part of a plant body of leafy vegetables that is in contact with soil or a medium and is not exposed to the air. This underground part has a function of absorbing nutrients and water necessary for growing leafy vegetables.
  • oxidized glutathione is absorbed from underground.
  • the subterranean part of the leafy vegetables comprises roots.
  • the “above-ground portion of leafy vegetables” refers to a portion of a plant body of leafy vegetables which is not in contact with soil or a medium and is exposed to the air.
  • This aerial part has the function of performing photosynthesis necessary for the growth of leafy vegetables.
  • the above-ground part is a part that is produced (cultivated) and then used for food.
  • the above-ground part of the leafy vegetables comprises leaves.
  • oxidized glutathione is applied underground.
  • the oxidized glutathione applied may be in any state as long as it can be absorbed via the underground part of the leafy vegetables.
  • the state of oxidized glutathione that can be applied by the method of the present invention may be, for example, an oxidized glutathione-containing solution obtained by dissolving oxidized glutathione in water or a water-soluble solvent (methanol, ethanol, etc.) or a mixed solvent thereof. ..
  • it may be a solid substance containing oxidized glutathione, such as powder or granules, which is a simple substance of oxidized glutathione or a mixture with other compounds.
  • this solution may contain a solvent such as a water-soluble solvent such as water, methanol or ethanol in addition to the oxidized glutathione. it can. Furthermore, this solution may contain components such as a surfactant (straight chain alkylbenzene sulfonate, etc.), a dispersion stabilizer (carboxymethyl cellulose, its salt, etc.), a thickener, an antioxidant and the like.
  • a surfactant straight chain alkylbenzene sulfonate, etc.
  • dispersion stabilizer carboxymethyl cellulose, its salt, etc.
  • thickener an antioxidant and the like.
  • the content concentration of the oxidized glutathione in the solution is a concentration that can be absorbed by leaf vegetables via the underground part. It is not particularly limited as long as it is one.
  • ppm (w / v) to 10000 ppm (w / v) is preferable, 4000 ppm (w / v) to 8000 ppm (w / v) is preferable, and 5000 ppm (w / v) to 7000 ppm (w / v) is more preferable, and 6000 ppm (w / v) is further preferable.
  • the applied oxidized glutathione is in the form of an aqueous solution.
  • the oxidized glutathione state in the method of the present invention is an oxidized glutathione-containing solid
  • the solid is a surfactant (straight chain alkylbenzene sulfonate, alkylnaphthalene sulfonate, lauryl sulfate, castor oil potassium salt). Soaps, etc.), dispersion stabilizers (carboxymethyl cellulose, salts thereof, etc.), excipients (lactose, etc.), disintegrants, thickeners, antioxidants and other components may be included.
  • the concentration of oxidized glutathione contained in the solid matter is not particularly limited as long as it is a concentration that can be absorbed from the underground portion of leafy vegetables, but at the time of application, it is converted into oxidized glutathione free form. It is preferable that the concentration is, for example, 0.01 to 50% by mass.
  • the above-mentioned application of oxidized glutathione can be carried out by immersing the underground portion of leafy vegetables in an oxidized glutathione-containing medium from the viewpoint of the absorption efficiency of oxidized glutathione.
  • the medium is a nutrient solution in the state of an aqueous solution of the fertilizer component and oxidized glutathione.
  • oxidized glutathione had various growth promoting effects in plants, but the effect was largely dependent on the application period of oxidized glutathione.
  • the application time of oxidized glutathione to leafy vegetables was examined, and when oxidized glutathione was applied within the seedling raising period from the day of germination to the 12th day after germination, leafy vegetables produced were It was found that the weight increased significantly.
  • photosynthesis has begun after germination, and if oxidized glutathione is applied during the seedling raising period, which is the preparatory stage of full-scale growth, it is one of the growth promoting effects. It is considered that the increase promoting effect is efficiently induced, which leads to an increase in the harvest weight of leafy vegetables.
  • the seedling raising period in the present invention is from the day when leafy vegetables germinate to the 12th day after germination.
  • the weight per leaf plant reached 0.2 to 0.5 g, and the true leaves were fully developed. Has become. ..
  • the total applied amount of oxidized glutathione within the above-mentioned seedling raising period is 0.375 mg (hereinafter referred to as “mg / strain”) to 1.5 mg / strain, and more preferably Is 0.375 mg / strain to 1.125 mg / strain, more preferably 0.375 mg / strain to 0.75 mg / strain, and even more preferably 0.5625 mg / strain.
  • the period of application of oxidized glutathione within the seedling raising period is 1 to 5 days, preferably 2 to 5 days, more preferably 3 to 5 days, still more preferably 3 to 4 days. Even more preferably, it is 3 days.
  • the application period is the period from the 7th day after germination to the 7th day or later.
  • oxidized glutathione is applied daily during the above application period.
  • oxidized glutathione is applied every day from the day of germination to day 9 to day 11.
  • the daily application rate of oxidized glutathione within the above-mentioned seedling raising period is 0.125 mg / strain to 0.5 mg / strain, more preferably 0.125 mg / strain to The amount is 0.375 mg / strain, more preferably 0.125 mg / strain to 0.25 mg / strain, and even more preferably 0.1875 mg / strain.
  • Oxidized glutathione within the growth period from the day of germination to day 12 or later
  • Oxidized glutathione in addition to the application of oxidized glutathione within the period of seedling raising, 12 days after germination Oxidized glutathione can be further applied within the growing period of. As a result, the weight of leafy vegetables produced can be increased.
  • the total application amount of oxidized glutathione within the growth period is 0.263 mg / strain to 2.63 mg / strain, more preferably 0.263 mg / strain to 1.
  • the amount is 98 mg / strain, more preferably 0.67 mg / strain to 1.98 mg / strain, and even more preferably 1.31 mg / strain.
  • the application period of oxidized glutathione within the above-mentioned growth period is from the 12th day from the day when the leafy vegetables germinate, from the weight of the leafy vegetables (the total weight of the above-ground portion and the underground portion). ) Reaches 28 to 40 g per share.
  • the application of oxidized glutathione can be carried out in one day or divided into a plurality of days within the above growth period.
  • the application of oxidized glutathione is carried out for a plurality of days within the above growth period.
  • the application of oxidized glutathione is carried out every 3 days within the above growth period.
  • the weight of leafy vegetables (the sum of the weights of the above-ground portion and the below-ground portion) is 2.9 to 5.
  • Oxidized glutathione is applied on the day when it reaches 3 g, and on the day when the weight of leafy vegetables (the sum of the weights of the above-ground part and the underground part) reaches 28 to 40 g per strain.
  • the daily application rate of oxidized glutathione during the above-mentioned growth period is 0.046 mg / strain to 1.5 mg / strain per leaf vegetable, more preferably 0.046 mg. / Strain to 1.13 mg / strain, more preferably 0.12 mg / strain to 1.13 mg / strain, and even more preferably 0.23 mg / strain to 0.75 mg / strain.
  • the daily application rate of oxidized glutathione on the 12th day from the germination day is 0.046 mg / strain to 0.46 mg / strain per leaf vegetable, and The amount is preferably 0.046 mg / strain to 0.35 mg / strain, more preferably 0.12 mg / strain to 0.35 mg / strain, and even more preferably 0.23 mg / strain.
  • the dose is 0.067 mg / strain to 0.67 mg / strain per leaf vegetable, more preferably 0.067 mg / strain to 0.5 mg / strain, further preferably 0.17 mg / strain to 0.5 mg / strain.
  • the strain and even more preferably 0.33 mg / strain.
  • the total application amount of oxidized glutathione within the seedling raising period is 1 It is set to 0.375 mg / strain to 1.5 mg / strain, more preferably 0.5625 mg / strain to 1.5 mg / strain, even more preferably 0.75 mg / strain to 1.5 mg / strain, and even more preferably It will be 1.125 mg / strain.
  • the daily application amount of oxidized glutathione within the seedling raising period Is 0.125 mg / strain to 0.5 mg / strain, more preferably 0.1875 mg / strain to 0.5 mg / strain, even more preferably 0.25 mg / strain to 0.5 mg / strain, and even more It is preferably 0.375 mg / strain.
  • the method for producing leafy vegetables according to the present invention can be carried out either under an ordinary open environment such as open-air cultivation or under a closed environment. Those skilled in the art can appropriately select these environmental conditions for producing leafy vegetables by the method of the present invention.
  • the method for producing leafy vegetables is carried out in a closed environment. In a closed environment, constant production efficiency and quality can be secured without being affected by environmental changes in the external environment. Further, the closed environment includes a semi-fully closed environment such as a greenhouse, or a completely closed environment in which the cultivation environment is completely shielded from the outside and the environmental conditions are strictly controlled.
  • the "medium” refers to a medium having a function of retaining and supplying nutrient components (nitrogen, phosphoric acid, potassium, etc.) and water necessary for production (cultivation) of leaf vegetables to leaf vegetables. It does not matter whether it is solid or solid. Examples thereof include soil and solid carriers used in an open environment or a semi-closed environment, and nutrient solutions used in a semi-closed environment or a completely closed environment. In a preferred embodiment of the present invention, the underground part of the leafy vegetables is immersed in the medium. ..
  • the medium is a nutrient solution in which nutrient components are dissolved in water.
  • the production of leafy vegetables using a nutrient solution is generally called hydroponics and can be carried out by a method known to those skilled in the art.
  • hydroponic culture the concentration of each nutrient in the nutrient solution can be easily controlled.
  • the concentration of nutrient components in the nutrient solution can be kept constant.
  • a nutrient solution having a usual mixing ratio used in the hydroponics of ordinary leafy vegetables can be used as the nutrient component of the nutrient solution in the method of the present invention.
  • the nutrient solution used in the method of the present invention may contain, for example, a large amount of essential element and a small amount of essential element for hydroponics of leafy vegetables.
  • the large amount of essential elements are, for example, 6 elements of nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur.
  • the trace essential elements are, for example, seven elements of iron, manganese, zinc, copper, molybdenum, boron, and chlorine.
  • the method for producing leafy vegetables can be carried out under a completely closed environment.
  • a completely closed environment there is an advantage that the leaf vegetables produced have a uniform quality.
  • the method of the present invention can be carried out in a plant factory.
  • the plant factory enables the year-round production of plants by performing environmental control and growth prediction based on the monitoring of the cultivation environment and growth among the facilities that control and grow the growth environment of plants.
  • a cultivation facility By carrying out the method of the present invention in a plant factory, it is possible to quantitatively and cyclically control the application amount and application timing of oxidized glutathione to leafy vegetables, thereby producing a large amount of leafy vegetables of constant quality. it can.
  • Leafy Vegetable Produced by the Method of the Present Invention As another aspect of the present invention, a leafy vegetable produced by the method of the present invention is provided.
  • Leaf vegetables produced by the method of the present invention the physiological state in the leaf vegetables caused by absorption of oxidized glutathione is determined by gene expression analysis or metabolome analysis of metabolites, and the results are compared. Thus, it can be distinguished from leafy vegetables to which oxidized glutathione is not applied.
  • the leafy vegetables produced by the method of the present invention are distinguished from leafy vegetables to which oxidized glutathione is not applied by examining the change in taste caused by absorption of oxidized glutathione in the leafy vegetables. be able to.
  • oxidized glutathione is applied to the underground part of leafy vegetables at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a method of increasing the yield of leafy vegetables comprising the steps of:
  • the increase in the yield of leafy vegetables is preferably an increase in the weight of the above-ground portion of the leafy vegetables.
  • the rate of increase in the weight of the above-ground portion of leafy vegetables is preferably 5% or more, more preferably 6% or more, further preferably 8% or more, more preferably 10% or more. Is more preferable, and 12% or more is particularly preferable.
  • the average value of the above-ground weight of leaf vegetables produced by applying oxidized glutathione is A, and the average above-ground weight of leaf vegetables produced under the same conditions except that oxidized glutathione is not applied.
  • the method of the present invention is advantageous in that the weight of leafy vegetables produced when grown for the same number of days increases. Further, according to the present invention, it is possible to reduce the number of production (cultivation) days required when the final weight of leaf vegetables produced is the same.
  • composition for increasing the yield of leafy vegetables Another aspect of the present invention is a composition for increasing the yield of leafy vegetables, comprising oxidized glutathione, at least on the day of germination.
  • the composition for increasing the yield of leafy vegetables comprises a fertilizer composition for leafy vegetables or a growth promoter for leafy vegetables.
  • the fertilizer composition for leafy vegetables of the present invention comprises oxidized glutathione.
  • it may further contain a fertilizer component generally used for leafy vegetables, as long as the action of the oxidized glutathione described above is not impaired.
  • the fertilizer component refers to an element used for leafy vegetables such as nitrogen, phosphorus, potassium, silicon, magnesium, calcium, manganese, boron, and iron.
  • the other fertilizer component of oxidized glutathione in the fertilizer composition for leaf vegetables of the present invention uses an organic compound containing a fertilizer component element, which is a form that can be used for leaf vegetables, an inorganic compound, or a combination thereof. be able to.
  • the fertilizer component used in the present invention preferably contains at least one selected from the group consisting of nitrogen, phosphorus, potassium, silicon, magnesium, calcium, manganese, boron and iron. From nitrogen, phosphorus and potassium More preferably, it comprises at least one selected from the group consisting of, and it is even more preferred that it contains at least a combination of three kinds of nitrogen, phosphorus and potassium.
  • the fertilizer composition for leafy vegetables of the present invention may be used in the above-mentioned method for producing leafy vegetables or a method for increasing the yield of leafy vegetables.
  • the growth promoter for leafy vegetables of the present invention comprises oxidized glutathione.
  • a plant hormone and / or a growth promoter may be further contained, as long as the action of the oxidized glutathione described above is not impaired.
  • examples of the plant hormone include gibberellin.
  • examples of the growth promoting agent include benzoic acid, nicotinic acid, nicotinic acid amide, pipecolic acid and the like.
  • the growth promoter of the present invention may be used in the method for producing leafy vegetables or the method for increasing the yield of leafy vegetables.
  • the present invention includes the following [1] to [20].
  • a method for producing leafy vegetables which comprises a step of applying oxidized glutathione to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination.
  • the method according to [1] or [2], wherein the total application amount of the oxidized glutathione within the seedling raising period is 0.375 to 1.5 mg per strain.
  • [5] The method according to any one of [1] to [4], wherein the application period is a period from the day of germination to the seventh day or later.
  • [6] The method according to [4] or [5], wherein oxidized glutathione is applied daily during the application period.
  • [7] The method according to any one of [4] to [6], wherein the daily application amount of the oxidized glutathione within the seedling raising period is 0.125 to 0.5 mg per strain.
  • the application of the oxidized glutathione comprises immersing the underground part of the leafy vegetable in an oxidized glutathione-containing medium.
  • [16] The method according to any one of [1] to [15], which is performed in a closed environment.
  • [17] The method according to any one of [1] to [16], which is performed in a plant factory.
  • the leafy vegetables are leaf lettuce, heading lettuce, leafy vegetables in the family Cruciferae, mustard greens (wasabi greens), Mizuna, leaf radish, cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage, The method according to any one of [1] to [17], which is selected from the group consisting of chard (Swiss chard), spinach and chrysanthemum.
  • Increasing the yield of leafy vegetables which comprises a step of applying oxidized glutathione to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination Method.
  • a composition for increasing the yield of leafy vegetables the composition comprising oxidized glutathione, the composition of leafy vegetables at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a composition for applying oxidized glutathione to the underground is
  • the use of oxidized glutathione for increasing the yield of leafy vegetables wherein the seedling raising period is at least from the day of germination to the 12th day after germination.
  • a use comprising applying oxidized glutathione to the basement of leafy vegetables.
  • the use of oxidized glutathione in the manufacture of a composition for increasing the yield of leafy vegetables comprising applying the composition to the basement of leafy vegetables within the seedling raising period from day to 12 days post germination.
  • the oxidized glutathione for increasing the yield of leafy vegetables which is a leafy material at least within the seedling raising period from the day of germination to the 12th day after germination.
  • Oxidized glutathione is provided which comprises applying oxidized glutathione to the subterranean portion of a vegetable.
  • a 4000K LED was used as an artificial light source for irradiation.
  • One day during hydroponics of frill lettuce was divided into a light period in which frill lettuce was irradiated with an artificial light source and a dark period in which it was not irradiated, and the time distribution was 16 hours for the light period and 8 hours for the dark period.
  • the temperature was 18 to 25 ° C. and the humidity was 50 to 90%. No pesticides were used.
  • Test example 1 The amount of harvest when the oxidized glutathione was applied to the frill lettuce for 3 days from the day of germination to the day 9 to 11 was confirmed.
  • Test plot 1-1 and control plot 1-1 In test section 1-1, hydroponic cultivation of frill lettuce was carried out by the method described in "1. Hydroponic cultivation of frill lettuce” above, and a total of 3 days from 9 to 11 days from the day on which the frill lettuce germinated. A new nutrient solution to which 1.5 ml of an aqueous glutathione solution (commercially available oxidized glutathione reagent was used. The concentration of oxidized glutathione was 6000 ppm; the same applies hereinafter) was added once a day for 1 day during the day. I replaced it with.
  • the amount added once a day corresponds to the amount of oxidized glutathione of 0.1875 mg (hereinafter referred to as "0.1875 mg / strain”) per furyl lettuce strain. Therefore, in test section 1-1, a total of 4.5 ml of an aqueous glutathione solution was added to the nutrient solution per 1 L of the nutrient solution. This total amount corresponds to the amount of oxidized glutathione of 0.5625 mg / strain.
  • Control Group 1-1 hydroponic cultivation of frill lettuce was performed simultaneously under the same conditions as in Test Group 1-1 except that the glutathione aqueous solution was not added.
  • Frill lettuce hydroponically cultivated in the test plot 1-1 and the control plot 1-1 were randomly harvested in 13 strains respectively, and the weight of the above-ground portion of the harvested furil lettuce was measured, and the average value was calculated. .. Furthermore, the relative value of the average value of the above-ground weight of furyl lettuce obtained in Test Group 1-1 when the average value of the above-ground weight of furyl lettuce obtained in Control Group 1-1 was set to 100 Calculated. The results are shown in Table 1.
  • Test plot 1-2 and control plot 1-2 In test area 1-2, during a total of 3 days on the 9th to 11th days after the germination of furyl lettuce, once a day, exchange with a new nutrient solution to which 3.0 ml of glutathione aqueous solution was added per 1 L of nutrient solution. Frill lettuce was hydroponically cultivated in the same manner as in Test Area 1-1 except for the above. The amount added once a day corresponds to the amount of oxidized glutathione of 0.375 mg / strain. Therefore, in test section 1-2, a total of 9.0 ml of an aqueous glutathione solution was added to the nutrient solution per 1 L of the nutrient solution. This total amount corresponds to 1.125 mg / strain of oxidized glutathione.
  • Control Group 1-2 hydroponic cultivation of frill lettuce was performed at the same time under the same conditions as in Test Group 1-2 except that the glutathione aqueous solution was not added.
  • test plot 1-1 when a sensory test on the taste and flavor of furil lettuce harvested by hydroponically cultivating furil lettuce under the same conditions as in test plot 1-1, no significant difference was found between the test plot and control plot. .. However, in some cultivation cases, the test plots sometimes felt sweeter than the control plots.
  • Test example 2 The amount of harvest when the oxidized glutathione was applied to the frill lettuce on and after the 12th day after germination was confirmed.
  • Test plot 2-1 and control plot 2-1 hydroponic cultivation of furil lettuce was carried out by the method described in "1. Hydroponic cultivation of furil lettuce” above, and 12 days after the germination of furil lettuce (post first addition) ) (Addition on and after the 12th day from germination is referred to as “post ... addition”; the same in the present specification), weight per frill lettuce strain (total sum of weight of above-ground portion and below-ground portion of furyl-lettuce) The amount per day (post 2nd addition), and the weight per frill lettuce strain (total sum of above-ground and below-ground parts) reached 28-40 g.
  • control plot 2-1 hydroponic cultivation of frill lettuce was performed at the same time as the test plot 2-1, under the same conditions as in the test plot 2-1, except that the aqueous glutathione solution was not added.
  • Test area 2-2 and control area 2-2 In test section 2-2, hydroponic cultivation of frill lettuce was performed by the method described in "1. Hydroponic cultivation of frill lettuce” above, and 12 days after the day when the frill lettuce germinated (post first addition) ) And frill lettuce per strain (sum of the weight of the above-ground part and the underground part) reached 2.9 to 5.3 g (post second addition), in each one addition, It was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the solution.
  • control plot 2-2 hydroponic cultivation of frill lettuce was performed at the same time as the test plot 2-2 under the same conditions as in the test plot 2-2 except that the glutathione aqueous solution was not added.
  • Test area 2-3 In test section 2-3, hydroponic cultivation of frill lettuce was carried out by the method described in "1. Hydroponic cultivation of frill lettuce" above, and 12 days from the day on which the frill lettuce germinated (post first addition) ) Was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution. In addition, on the day when the weight (total sum of the weight of the above-ground part and the amount of the below-ground part) of the frill lettuce reached 2.9 to 5.3 g (post second addition), 1 ml of glutathione aqueous solution was added per 1 L of the nutrient solution. The new nutrient solution added was replaced.
  • control plot 2-3 hydroponic cultivation of frill lettuce was carried out at the same time as test plot 2-2 under the same conditions as in test plot 2-3 except that the glutathione aqueous solution was not added.
  • Test example 3 Yield when oxidized glutathione was applied to frill lettuce for 3 to 9 to 11 days after germination, and glutathione aqueous solution was applied in multiple times from 12 to 12 days after germination. It was confirmed.
  • Test area 3-1 In test section 3-1, hydroponic cultivation of frill lettuce was carried out by the method described in “1. Hydroponic cultivation of frill lettuce” above, and a total of 3 days from the day when the frill lettuce germinated to the 11th to 11th days. During the day, the fresh nutrient solution was added once a day with 3.0 ml of an aqueous glutathione solution added per liter of the nutrient solution. The amount added once a day corresponds to the amount of oxidized glutathione of 0.375 mg / strain.
  • the weight of the furyl lettuce per strain (the total weight of the above-ground portion and the underground portion) was 2.9 to 12 days after the germination of the furyl lettuce (the first post addition).
  • the weight of the furyl lettuce per strain (sum of the weight of the above-ground part and the underground part) reached 28-40 g (post third addition)
  • a new nutrient solution containing 0.5 ml of an aqueous glutathione solution per 1 L of nutrient solution was replaced.
  • the amount added at the 1st to 3rd post corresponds to the amount of oxidized glutathione at 0.23 mg / strain (first post), 0.33 mg / strain (second post), 0.75 mg / strain (third post). To do.
  • control section 3-1 hydroponic cultivation of frill lettuce was carried out at the same time as the test section 3-1 under the same conditions as in the test section 3-1, except that the aqueous glutathione solution was not added.
  • Test plot 3-2 and control plot 3-2 In test area 3-2, hydroponic cultivation of furil lettuce was carried out by the method described in “1. Hydroponic cultivation method of furil lettuce”, and a total of 3 days from the day when germinated furil lettuce was germinated. During the day, the fresh nutrient solution was added once a day with the addition of 1.5 ml of glutathione aqueous solution per liter of nutrient solution. The amount added once a day corresponds to the amount of oxidized glutathione of 0.1875 mg / strain.
  • the weight per 1 day of the furyl lettuce was 2.9 to 12 days after the germination of the furyl lettuce (first addition of post).
  • the weight of the furyl lettuce per strain (sum of aboveground and underground parts) reached 28-40 g (post third addition), respectively.
  • it was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution.
  • the post-first to third addition amounts were 0.23 mg / strain (post first addition), 0.33 mg / strain (post second addition), and 0.75 mg / strain (post third addition) oxidized form, respectively. Equivalent to the amount of glutathione.
  • control section 3-2 hydroponic cultivation of frill lettuce was carried out at the same time as test section 3-2 under the same conditions as in test section 3-2 except that the glutathione aqueous solution was not added.
  • the glutathione aqueous solution was obtained on the 9th to 11th days after the germination of the frill lettuce and on the 12th day after the germination of the frill lettuce. As a result of the addition of a plurality of times, the weight of the harvested frill lettuce was increased. ..
  • test was conducted in the same manner as in Test Group 3-1 except that the addition amount of glutathione was changed to 5.0 ml per 1 L of the nutrient solution once a day for 3 to 9 to 11 days from the day on which the frill lettuce germinated. I went. As a result, no sales-increasing effect of frill lettuce was observed.

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Abstract

La présente invention concerne un procédé de production de légumes à feuilles ayant une excellente efficacité de production. Plus en détail, l'invention concerne un procédé de production de légumes à feuilles comprenant une étape consistant à appliquer, au moins au cours d'une période de croissance de semis à partir du jour où la germination s'est produite jusqu'au douzième jour après la germination, du glutathion oxydé sur la partie souterraine d'un légume à feuilles.
PCT/JP2019/045327 2018-11-20 2019-11-19 Procédé de production de légumes à feuilles WO2020105649A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023171639A1 (fr) * 2022-03-07 2023-09-14 株式会社カネカ Composition agricole et procédé de culture de plantes l'utilisant

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008072602A1 (fr) * 2006-12-11 2008-06-19 Japan Science And Technology Agency Régulateur de croissance végétale et son utilisation
WO2013088956A1 (fr) * 2011-12-12 2013-06-20 岡山県 Composé augmentant la teneur en acides aminés dans un plant et utilisation du composé
WO2017006869A1 (fr) * 2015-07-03 2017-01-12 株式会社カネカ Composition d'engrais pour une application sur les feuilles et comprenant du glutathion oxydé et un constituant d'engrais
JP2017063632A (ja) * 2015-09-28 2017-04-06 公立大学法人秋田県立大学 水耕栽培方法、葉菜類、培養液、及び培養液濃縮組成物
JP2018121590A (ja) * 2017-02-02 2018-08-09 昭和電工株式会社 人工光による育苗方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008072602A1 (fr) * 2006-12-11 2008-06-19 Japan Science And Technology Agency Régulateur de croissance végétale et son utilisation
WO2013088956A1 (fr) * 2011-12-12 2013-06-20 岡山県 Composé augmentant la teneur en acides aminés dans un plant et utilisation du composé
WO2017006869A1 (fr) * 2015-07-03 2017-01-12 株式会社カネカ Composition d'engrais pour une application sur les feuilles et comprenant du glutathion oxydé et un constituant d'engrais
JP2017063632A (ja) * 2015-09-28 2017-04-06 公立大学法人秋田県立大学 水耕栽培方法、葉菜類、培養液、及び培養液濃縮組成物
JP2018121590A (ja) * 2017-02-02 2018-08-09 昭和電工株式会社 人工光による育苗方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023171639A1 (fr) * 2022-03-07 2023-09-14 株式会社カネカ Composition agricole et procédé de culture de plantes l'utilisant

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