WO2020105649A1 - Leaf vegetable production method - Google Patents

Leaf vegetable production method

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Publication number
WO2020105649A1
WO2020105649A1 PCT/JP2019/045327 JP2019045327W WO2020105649A1 WO 2020105649 A1 WO2020105649 A1 WO 2020105649A1 JP 2019045327 W JP2019045327 W JP 2019045327W WO 2020105649 A1 WO2020105649 A1 WO 2020105649A1
Authority
WO
WIPO (PCT)
Prior art keywords
day
oxidized glutathione
germination
leafy vegetables
period
Prior art date
Application number
PCT/JP2019/045327
Other languages
French (fr)
Japanese (ja)
Inventor
小川　健一
靖史 大門
季之 高橋
功英 石橋
Original Assignee
岡山県
株式会社アースサイド
Ｊｘｔｇエネルギー株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 岡山県, 株式会社アースサイド, Ｊｘｔｇエネルギー株式会社 filed Critical 岡山県
Publication of WO2020105649A1 publication Critical patent/WO2020105649A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides

Definitions

  • the present invention relates to a method for producing leafy vegetables.
  • Glutathione is a peptide consisting of three amino acids, L-cysteine, L-glutamic acid, and glycine, which exists not only in the human body, but in many living organisms such as other animals, plants, and microorganisms, and removes active oxygen. Functions such as action and detoxification of peroxide are known.
  • Glutathione is composed of reduced glutathione, which is present as a single peptide molecule consisting of three amino acids, L-cysteine, L-glutamic acid, and glycine, and oxidized glutathione, in which two molecules of reduced glutathione are bound via a disulfide bond. It exists in either form.
  • Oxidized glutathione is known to be useful in the fertilizer field because it has a function of promoting plant growth. For example, it has been reported that the number of seeds or flowers of a plant is increased by administering a plant with oxidized glutathione (Patent Document 1). Further, it has been reported that the growth of tubers and the like was promoted by administering oxidized glutathione to tubers and the like in the bud stage (Patent Document 2). Furthermore, it has been reported that changes in growth and zinc content were observed when oxidized glutathione was administered to leaf lettuce (Patent Document 3).
  • the present invention provides a method for producing leafy vegetables having excellent production efficiency.
  • leaf vegetables are produced, which comprises a step of applying oxidized glutathione to the underground part of leaf vegetables at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a method is provided.
  • the step of applying oxidized glutathione to the underground portion of the leaf vegetables is used.
  • a method of increasing yield is provided.
  • a composition for increasing the yield of leafy vegetables comprising oxidized glutathione, at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a composition for applying oxidized glutathione to the underground part of leafy vegetables comprising oxidized glutathione, at least within the seedling raising period from the day of germination to the 12th day after germination.
  • the present invention it is advantageous in that the weight of leaf vegetables produced is increased. Further, according to the present invention, it is possible to reduce the number of production (cultivation) days required when the final weight of leaf vegetables produced is the same.
  • oxidized glutathione means reduced glutathione (N- (N- ⁇ -L-glutamyl-L-cysteinyl) glycine). It is a compound in which two molecules are bound via a disulfide bond.
  • oxidized glutathione is not bound to other substances and is not an ionized educt, a salt formed with oxidized glutathione and an acid or a base, a hydrate of these, Various forms such as a mixture may be included.
  • the above-mentioned oxidized glutathione salt is not particularly limited as long as it is a salt acceptable as a fertilizer such as ammonium salt, calcium salt, magnesium salt, sodium salt, lithium salt and the like.
  • the salt of oxidized glutathione is preferably one or more salts selected from ammonium salts, calcium salts and magnesium salts.
  • the leafy vegetables produced by the method of the present invention may be of any type as long as they are vegetables whose leaf portion is mainly edible.
  • leafy vegetables produced in the present invention include ruffle lettuce, romaine lettuce, batavia lettuce, lorobionda, lolorossa, butterhead lettuce, leaf lettuce such as oakley lettuce; headlet lettuce; mustard vegetable (wasabi vegetable), Mizuna , Leaf radish, Chinese cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage and other cruciferous leafy vegetables, chard (Swiss chard), spinach (mainly for salad variety), or other such as chrysanthemum Examples include leafy vegetables.
  • leaf lettuce such as frill lettuce, romaine lettuce, Batavia lettuce, lorobinonda, lolorossa, butterhead lettuce, or oakley lettuce is preferable.
  • the seeds of leafy vegetables or seedlings immediately after germination used for the production of leafy vegetables can be obtained by methods known to those skilled in the art.
  • the “underground part of leafy vegetables” refers to a part of a plant body of leafy vegetables that is in contact with soil or a medium and is not exposed to the air. This underground part has a function of absorbing nutrients and water necessary for growing leafy vegetables.
  • oxidized glutathione is absorbed from underground.
  • the subterranean part of the leafy vegetables comprises roots.
  • the “above-ground portion of leafy vegetables” refers to a portion of a plant body of leafy vegetables which is not in contact with soil or a medium and is exposed to the air.
  • This aerial part has the function of performing photosynthesis necessary for the growth of leafy vegetables.
  • the above-ground part is a part that is produced (cultivated) and then used for food.
  • the above-ground part of the leafy vegetables comprises leaves.
  • oxidized glutathione is applied underground.
  • the oxidized glutathione applied may be in any state as long as it can be absorbed via the underground part of the leafy vegetables.
  • the state of oxidized glutathione that can be applied by the method of the present invention may be, for example, an oxidized glutathione-containing solution obtained by dissolving oxidized glutathione in water or a water-soluble solvent (methanol, ethanol, etc.) or a mixed solvent thereof. ..
  • it may be a solid substance containing oxidized glutathione, such as powder or granules, which is a simple substance of oxidized glutathione or a mixture with other compounds.
  • this solution may contain a solvent such as a water-soluble solvent such as water, methanol or ethanol in addition to the oxidized glutathione. it can. Furthermore, this solution may contain components such as a surfactant (straight chain alkylbenzene sulfonate, etc.), a dispersion stabilizer (carboxymethyl cellulose, its salt, etc.), a thickener, an antioxidant and the like.
  • a surfactant straight chain alkylbenzene sulfonate, etc.
  • dispersion stabilizer carboxymethyl cellulose, its salt, etc.
  • thickener an antioxidant and the like.
  • the content concentration of the oxidized glutathione in the solution is a concentration that can be absorbed by leaf vegetables via the underground part. It is not particularly limited as long as it is one.
  • ppm (w / v) to 10000 ppm (w / v) is preferable, 4000 ppm (w / v) to 8000 ppm (w / v) is preferable, and 5000 ppm (w / v) to 7000 ppm (w / v) is more preferable, and 6000 ppm (w / v) is further preferable.
  • the applied oxidized glutathione is in the form of an aqueous solution.
  • the oxidized glutathione state in the method of the present invention is an oxidized glutathione-containing solid
  • the solid is a surfactant (straight chain alkylbenzene sulfonate, alkylnaphthalene sulfonate, lauryl sulfate, castor oil potassium salt). Soaps, etc.), dispersion stabilizers (carboxymethyl cellulose, salts thereof, etc.), excipients (lactose, etc.), disintegrants, thickeners, antioxidants and other components may be included.
  • the concentration of oxidized glutathione contained in the solid matter is not particularly limited as long as it is a concentration that can be absorbed from the underground portion of leafy vegetables, but at the time of application, it is converted into oxidized glutathione free form. It is preferable that the concentration is, for example, 0.01 to 50% by mass.
  • the above-mentioned application of oxidized glutathione can be carried out by immersing the underground portion of leafy vegetables in an oxidized glutathione-containing medium from the viewpoint of the absorption efficiency of oxidized glutathione.
  • the medium is a nutrient solution in the state of an aqueous solution of the fertilizer component and oxidized glutathione.
  • oxidized glutathione had various growth promoting effects in plants, but the effect was largely dependent on the application period of oxidized glutathione.
  • the application time of oxidized glutathione to leafy vegetables was examined, and when oxidized glutathione was applied within the seedling raising period from the day of germination to the 12th day after germination, leafy vegetables produced were It was found that the weight increased significantly.
  • photosynthesis has begun after germination, and if oxidized glutathione is applied during the seedling raising period, which is the preparatory stage of full-scale growth, it is one of the growth promoting effects. It is considered that the increase promoting effect is efficiently induced, which leads to an increase in the harvest weight of leafy vegetables.
  • the seedling raising period in the present invention is from the day when leafy vegetables germinate to the 12th day after germination.
  • the weight per leaf plant reached 0.2 to 0.5 g, and the true leaves were fully developed. Has become. ..
  • the total applied amount of oxidized glutathione within the above-mentioned seedling raising period is 0.375 mg (hereinafter referred to as “mg / strain”) to 1.5 mg / strain, and more preferably Is 0.375 mg / strain to 1.125 mg / strain, more preferably 0.375 mg / strain to 0.75 mg / strain, and even more preferably 0.5625 mg / strain.
  • the period of application of oxidized glutathione within the seedling raising period is 1 to 5 days, preferably 2 to 5 days, more preferably 3 to 5 days, still more preferably 3 to 4 days. Even more preferably, it is 3 days.
  • the application period is the period from the 7th day after germination to the 7th day or later.
  • oxidized glutathione is applied daily during the above application period.
  • oxidized glutathione is applied every day from the day of germination to day 9 to day 11.
  • the daily application rate of oxidized glutathione within the above-mentioned seedling raising period is 0.125 mg / strain to 0.5 mg / strain, more preferably 0.125 mg / strain to The amount is 0.375 mg / strain, more preferably 0.125 mg / strain to 0.25 mg / strain, and even more preferably 0.1875 mg / strain.
  • Oxidized glutathione within the growth period from the day of germination to day 12 or later
  • Oxidized glutathione in addition to the application of oxidized glutathione within the period of seedling raising, 12 days after germination Oxidized glutathione can be further applied within the growing period of. As a result, the weight of leafy vegetables produced can be increased.
  • the total application amount of oxidized glutathione within the growth period is 0.263 mg / strain to 2.63 mg / strain, more preferably 0.263 mg / strain to 1.
  • the amount is 98 mg / strain, more preferably 0.67 mg / strain to 1.98 mg / strain, and even more preferably 1.31 mg / strain.
  • the application period of oxidized glutathione within the above-mentioned growth period is from the 12th day from the day when the leafy vegetables germinate, from the weight of the leafy vegetables (the total weight of the above-ground portion and the underground portion). ) Reaches 28 to 40 g per share.
  • the application of oxidized glutathione can be carried out in one day or divided into a plurality of days within the above growth period.
  • the application of oxidized glutathione is carried out for a plurality of days within the above growth period.
  • the application of oxidized glutathione is carried out every 3 days within the above growth period.
  • the weight of leafy vegetables (the sum of the weights of the above-ground portion and the below-ground portion) is 2.9 to 5.
  • Oxidized glutathione is applied on the day when it reaches 3 g, and on the day when the weight of leafy vegetables (the sum of the weights of the above-ground part and the underground part) reaches 28 to 40 g per strain.
  • the daily application rate of oxidized glutathione during the above-mentioned growth period is 0.046 mg / strain to 1.5 mg / strain per leaf vegetable, more preferably 0.046 mg. / Strain to 1.13 mg / strain, more preferably 0.12 mg / strain to 1.13 mg / strain, and even more preferably 0.23 mg / strain to 0.75 mg / strain.
  • the daily application rate of oxidized glutathione on the 12th day from the germination day is 0.046 mg / strain to 0.46 mg / strain per leaf vegetable, and The amount is preferably 0.046 mg / strain to 0.35 mg / strain, more preferably 0.12 mg / strain to 0.35 mg / strain, and even more preferably 0.23 mg / strain.
  • the dose is 0.067 mg / strain to 0.67 mg / strain per leaf vegetable, more preferably 0.067 mg / strain to 0.5 mg / strain, further preferably 0.17 mg / strain to 0.5 mg / strain.
  • the strain and even more preferably 0.33 mg / strain.
  • the total application amount of oxidized glutathione within the seedling raising period is 1 It is set to 0.375 mg / strain to 1.5 mg / strain, more preferably 0.5625 mg / strain to 1.5 mg / strain, even more preferably 0.75 mg / strain to 1.5 mg / strain, and even more preferably It will be 1.125 mg / strain.
  • the daily application amount of oxidized glutathione within the seedling raising period Is 0.125 mg / strain to 0.5 mg / strain, more preferably 0.1875 mg / strain to 0.5 mg / strain, even more preferably 0.25 mg / strain to 0.5 mg / strain, and even more It is preferably 0.375 mg / strain.
  • the method for producing leafy vegetables according to the present invention can be carried out either under an ordinary open environment such as open-air cultivation or under a closed environment. Those skilled in the art can appropriately select these environmental conditions for producing leafy vegetables by the method of the present invention.
  • the method for producing leafy vegetables is carried out in a closed environment. In a closed environment, constant production efficiency and quality can be secured without being affected by environmental changes in the external environment. Further, the closed environment includes a semi-fully closed environment such as a greenhouse, or a completely closed environment in which the cultivation environment is completely shielded from the outside and the environmental conditions are strictly controlled.
  • the "medium” refers to a medium having a function of retaining and supplying nutrient components (nitrogen, phosphoric acid, potassium, etc.) and water necessary for production (cultivation) of leaf vegetables to leaf vegetables. It does not matter whether it is solid or solid. Examples thereof include soil and solid carriers used in an open environment or a semi-closed environment, and nutrient solutions used in a semi-closed environment or a completely closed environment. In a preferred embodiment of the present invention, the underground part of the leafy vegetables is immersed in the medium. ..
  • the medium is a nutrient solution in which nutrient components are dissolved in water.
  • the production of leafy vegetables using a nutrient solution is generally called hydroponics and can be carried out by a method known to those skilled in the art.
  • hydroponic culture the concentration of each nutrient in the nutrient solution can be easily controlled.
  • the concentration of nutrient components in the nutrient solution can be kept constant.
  • a nutrient solution having a usual mixing ratio used in the hydroponics of ordinary leafy vegetables can be used as the nutrient component of the nutrient solution in the method of the present invention.
  • the nutrient solution used in the method of the present invention may contain, for example, a large amount of essential element and a small amount of essential element for hydroponics of leafy vegetables.
  • the large amount of essential elements are, for example, 6 elements of nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur.
  • the trace essential elements are, for example, seven elements of iron, manganese, zinc, copper, molybdenum, boron, and chlorine.
  • the method for producing leafy vegetables can be carried out under a completely closed environment.
  • a completely closed environment there is an advantage that the leaf vegetables produced have a uniform quality.
  • the method of the present invention can be carried out in a plant factory.
  • the plant factory enables the year-round production of plants by performing environmental control and growth prediction based on the monitoring of the cultivation environment and growth among the facilities that control and grow the growth environment of plants.
  • a cultivation facility By carrying out the method of the present invention in a plant factory, it is possible to quantitatively and cyclically control the application amount and application timing of oxidized glutathione to leafy vegetables, thereby producing a large amount of leafy vegetables of constant quality. it can.
  • Leafy Vegetable Produced by the Method of the Present Invention As another aspect of the present invention, a leafy vegetable produced by the method of the present invention is provided.
  • Leaf vegetables produced by the method of the present invention the physiological state in the leaf vegetables caused by absorption of oxidized glutathione is determined by gene expression analysis or metabolome analysis of metabolites, and the results are compared. Thus, it can be distinguished from leafy vegetables to which oxidized glutathione is not applied.
  • the leafy vegetables produced by the method of the present invention are distinguished from leafy vegetables to which oxidized glutathione is not applied by examining the change in taste caused by absorption of oxidized glutathione in the leafy vegetables. be able to.
  • oxidized glutathione is applied to the underground part of leafy vegetables at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a method of increasing the yield of leafy vegetables comprising the steps of:
  • the increase in the yield of leafy vegetables is preferably an increase in the weight of the above-ground portion of the leafy vegetables.
  • the rate of increase in the weight of the above-ground portion of leafy vegetables is preferably 5% or more, more preferably 6% or more, further preferably 8% or more, more preferably 10% or more. Is more preferable, and 12% or more is particularly preferable.
  • the average value of the above-ground weight of leaf vegetables produced by applying oxidized glutathione is A, and the average above-ground weight of leaf vegetables produced under the same conditions except that oxidized glutathione is not applied.
  • the method of the present invention is advantageous in that the weight of leafy vegetables produced when grown for the same number of days increases. Further, according to the present invention, it is possible to reduce the number of production (cultivation) days required when the final weight of leaf vegetables produced is the same.
  • composition for increasing the yield of leafy vegetables Another aspect of the present invention is a composition for increasing the yield of leafy vegetables, comprising oxidized glutathione, at least on the day of germination.
  • the composition for increasing the yield of leafy vegetables comprises a fertilizer composition for leafy vegetables or a growth promoter for leafy vegetables.
  • the fertilizer composition for leafy vegetables of the present invention comprises oxidized glutathione.
  • it may further contain a fertilizer component generally used for leafy vegetables, as long as the action of the oxidized glutathione described above is not impaired.
  • the fertilizer component refers to an element used for leafy vegetables such as nitrogen, phosphorus, potassium, silicon, magnesium, calcium, manganese, boron, and iron.
  • the other fertilizer component of oxidized glutathione in the fertilizer composition for leaf vegetables of the present invention uses an organic compound containing a fertilizer component element, which is a form that can be used for leaf vegetables, an inorganic compound, or a combination thereof. be able to.
  • the fertilizer component used in the present invention preferably contains at least one selected from the group consisting of nitrogen, phosphorus, potassium, silicon, magnesium, calcium, manganese, boron and iron. From nitrogen, phosphorus and potassium More preferably, it comprises at least one selected from the group consisting of, and it is even more preferred that it contains at least a combination of three kinds of nitrogen, phosphorus and potassium.
  • the fertilizer composition for leafy vegetables of the present invention may be used in the above-mentioned method for producing leafy vegetables or a method for increasing the yield of leafy vegetables.
  • the growth promoter for leafy vegetables of the present invention comprises oxidized glutathione.
  • a plant hormone and / or a growth promoter may be further contained, as long as the action of the oxidized glutathione described above is not impaired.
  • examples of the plant hormone include gibberellin.
  • examples of the growth promoting agent include benzoic acid, nicotinic acid, nicotinic acid amide, pipecolic acid and the like.
  • the growth promoter of the present invention may be used in the method for producing leafy vegetables or the method for increasing the yield of leafy vegetables.
  • the present invention includes the following [1] to [20].
  • a method for producing leafy vegetables which comprises a step of applying oxidized glutathione to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination.
  • the method according to [1] or [2], wherein the total application amount of the oxidized glutathione within the seedling raising period is 0.375 to 1.5 mg per strain.
  • [5] The method according to any one of [1] to [4], wherein the application period is a period from the day of germination to the seventh day or later.
  • [6] The method according to [4] or [5], wherein oxidized glutathione is applied daily during the application period.
  • [7] The method according to any one of [4] to [6], wherein the daily application amount of the oxidized glutathione within the seedling raising period is 0.125 to 0.5 mg per strain.
  • the application of the oxidized glutathione comprises immersing the underground part of the leafy vegetable in an oxidized glutathione-containing medium.
  • [16] The method according to any one of [1] to [15], which is performed in a closed environment.
  • [17] The method according to any one of [1] to [16], which is performed in a plant factory.
  • the leafy vegetables are leaf lettuce, heading lettuce, leafy vegetables in the family Cruciferae, mustard greens (wasabi greens), Mizuna, leaf radish, cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage, The method according to any one of [1] to [17], which is selected from the group consisting of chard (Swiss chard), spinach and chrysanthemum.
  • Increasing the yield of leafy vegetables which comprises a step of applying oxidized glutathione to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination Method.
  • a composition for increasing the yield of leafy vegetables the composition comprising oxidized glutathione, the composition of leafy vegetables at least within the seedling raising period from the day of germination to the 12th day after germination.
  • a composition for applying oxidized glutathione to the underground is
  • the use of oxidized glutathione for increasing the yield of leafy vegetables wherein the seedling raising period is at least from the day of germination to the 12th day after germination.
  • a use comprising applying oxidized glutathione to the basement of leafy vegetables.
  • the use of oxidized glutathione in the manufacture of a composition for increasing the yield of leafy vegetables comprising applying the composition to the basement of leafy vegetables within the seedling raising period from day to 12 days post germination.
  • the oxidized glutathione for increasing the yield of leafy vegetables which is a leafy material at least within the seedling raising period from the day of germination to the 12th day after germination.
  • Oxidized glutathione is provided which comprises applying oxidized glutathione to the subterranean portion of a vegetable.
  • a 4000K LED was used as an artificial light source for irradiation.
  • One day during hydroponics of frill lettuce was divided into a light period in which frill lettuce was irradiated with an artificial light source and a dark period in which it was not irradiated, and the time distribution was 16 hours for the light period and 8 hours for the dark period.
  • the temperature was 18 to 25 ° C. and the humidity was 50 to 90%. No pesticides were used.
  • Test example 1 The amount of harvest when the oxidized glutathione was applied to the frill lettuce for 3 days from the day of germination to the day 9 to 11 was confirmed.
  • Test plot 1-1 and control plot 1-1 In test section 1-1, hydroponic cultivation of frill lettuce was carried out by the method described in "1. Hydroponic cultivation of frill lettuce” above, and a total of 3 days from 9 to 11 days from the day on which the frill lettuce germinated. A new nutrient solution to which 1.5 ml of an aqueous glutathione solution (commercially available oxidized glutathione reagent was used. The concentration of oxidized glutathione was 6000 ppm; the same applies hereinafter) was added once a day for 1 day during the day. I replaced it with.
  • the amount added once a day corresponds to the amount of oxidized glutathione of 0.1875 mg (hereinafter referred to as "0.1875 mg / strain”) per furyl lettuce strain. Therefore, in test section 1-1, a total of 4.5 ml of an aqueous glutathione solution was added to the nutrient solution per 1 L of the nutrient solution. This total amount corresponds to the amount of oxidized glutathione of 0.5625 mg / strain.
  • Control Group 1-1 hydroponic cultivation of frill lettuce was performed simultaneously under the same conditions as in Test Group 1-1 except that the glutathione aqueous solution was not added.
  • Frill lettuce hydroponically cultivated in the test plot 1-1 and the control plot 1-1 were randomly harvested in 13 strains respectively, and the weight of the above-ground portion of the harvested furil lettuce was measured, and the average value was calculated. .. Furthermore, the relative value of the average value of the above-ground weight of furyl lettuce obtained in Test Group 1-1 when the average value of the above-ground weight of furyl lettuce obtained in Control Group 1-1 was set to 100 Calculated. The results are shown in Table 1.
  • Test plot 1-2 and control plot 1-2 In test area 1-2, during a total of 3 days on the 9th to 11th days after the germination of furyl lettuce, once a day, exchange with a new nutrient solution to which 3.0 ml of glutathione aqueous solution was added per 1 L of nutrient solution. Frill lettuce was hydroponically cultivated in the same manner as in Test Area 1-1 except for the above. The amount added once a day corresponds to the amount of oxidized glutathione of 0.375 mg / strain. Therefore, in test section 1-2, a total of 9.0 ml of an aqueous glutathione solution was added to the nutrient solution per 1 L of the nutrient solution. This total amount corresponds to 1.125 mg / strain of oxidized glutathione.
  • Control Group 1-2 hydroponic cultivation of frill lettuce was performed at the same time under the same conditions as in Test Group 1-2 except that the glutathione aqueous solution was not added.
  • test plot 1-1 when a sensory test on the taste and flavor of furil lettuce harvested by hydroponically cultivating furil lettuce under the same conditions as in test plot 1-1, no significant difference was found between the test plot and control plot. .. However, in some cultivation cases, the test plots sometimes felt sweeter than the control plots.
  • Test example 2 The amount of harvest when the oxidized glutathione was applied to the frill lettuce on and after the 12th day after germination was confirmed.
  • Test plot 2-1 and control plot 2-1 hydroponic cultivation of furil lettuce was carried out by the method described in "1. Hydroponic cultivation of furil lettuce” above, and 12 days after the germination of furil lettuce (post first addition) ) (Addition on and after the 12th day from germination is referred to as “post ... addition”; the same in the present specification), weight per frill lettuce strain (total sum of weight of above-ground portion and below-ground portion of furyl-lettuce) The amount per day (post 2nd addition), and the weight per frill lettuce strain (total sum of above-ground and below-ground parts) reached 28-40 g.
  • control plot 2-1 hydroponic cultivation of frill lettuce was performed at the same time as the test plot 2-1, under the same conditions as in the test plot 2-1, except that the aqueous glutathione solution was not added.
  • Test area 2-2 and control area 2-2 In test section 2-2, hydroponic cultivation of frill lettuce was performed by the method described in "1. Hydroponic cultivation of frill lettuce” above, and 12 days after the day when the frill lettuce germinated (post first addition) ) And frill lettuce per strain (sum of the weight of the above-ground part and the underground part) reached 2.9 to 5.3 g (post second addition), in each one addition, It was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the solution.
  • control plot 2-2 hydroponic cultivation of frill lettuce was performed at the same time as the test plot 2-2 under the same conditions as in the test plot 2-2 except that the glutathione aqueous solution was not added.
  • Test area 2-3 In test section 2-3, hydroponic cultivation of frill lettuce was carried out by the method described in "1. Hydroponic cultivation of frill lettuce" above, and 12 days from the day on which the frill lettuce germinated (post first addition) ) Was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution. In addition, on the day when the weight (total sum of the weight of the above-ground part and the amount of the below-ground part) of the frill lettuce reached 2.9 to 5.3 g (post second addition), 1 ml of glutathione aqueous solution was added per 1 L of the nutrient solution. The new nutrient solution added was replaced.
  • control plot 2-3 hydroponic cultivation of frill lettuce was carried out at the same time as test plot 2-2 under the same conditions as in test plot 2-3 except that the glutathione aqueous solution was not added.
  • Test example 3 Yield when oxidized glutathione was applied to frill lettuce for 3 to 9 to 11 days after germination, and glutathione aqueous solution was applied in multiple times from 12 to 12 days after germination. It was confirmed.
  • Test area 3-1 In test section 3-1, hydroponic cultivation of frill lettuce was carried out by the method described in “1. Hydroponic cultivation of frill lettuce” above, and a total of 3 days from the day when the frill lettuce germinated to the 11th to 11th days. During the day, the fresh nutrient solution was added once a day with 3.0 ml of an aqueous glutathione solution added per liter of the nutrient solution. The amount added once a day corresponds to the amount of oxidized glutathione of 0.375 mg / strain.
  • the weight of the furyl lettuce per strain (the total weight of the above-ground portion and the underground portion) was 2.9 to 12 days after the germination of the furyl lettuce (the first post addition).
  • the weight of the furyl lettuce per strain (sum of the weight of the above-ground part and the underground part) reached 28-40 g (post third addition)
  • a new nutrient solution containing 0.5 ml of an aqueous glutathione solution per 1 L of nutrient solution was replaced.
  • the amount added at the 1st to 3rd post corresponds to the amount of oxidized glutathione at 0.23 mg / strain (first post), 0.33 mg / strain (second post), 0.75 mg / strain (third post). To do.
  • control section 3-1 hydroponic cultivation of frill lettuce was carried out at the same time as the test section 3-1 under the same conditions as in the test section 3-1, except that the aqueous glutathione solution was not added.
  • Test plot 3-2 and control plot 3-2 In test area 3-2, hydroponic cultivation of furil lettuce was carried out by the method described in “1. Hydroponic cultivation method of furil lettuce”, and a total of 3 days from the day when germinated furil lettuce was germinated. During the day, the fresh nutrient solution was added once a day with the addition of 1.5 ml of glutathione aqueous solution per liter of nutrient solution. The amount added once a day corresponds to the amount of oxidized glutathione of 0.1875 mg / strain.
  • the weight per 1 day of the furyl lettuce was 2.9 to 12 days after the germination of the furyl lettuce (first addition of post).
  • the weight of the furyl lettuce per strain (sum of aboveground and underground parts) reached 28-40 g (post third addition), respectively.
  • it was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution.
  • the post-first to third addition amounts were 0.23 mg / strain (post first addition), 0.33 mg / strain (post second addition), and 0.75 mg / strain (post third addition) oxidized form, respectively. Equivalent to the amount of glutathione.
  • control section 3-2 hydroponic cultivation of frill lettuce was carried out at the same time as test section 3-2 under the same conditions as in test section 3-2 except that the glutathione aqueous solution was not added.
  • the glutathione aqueous solution was obtained on the 9th to 11th days after the germination of the frill lettuce and on the 12th day after the germination of the frill lettuce. As a result of the addition of a plurality of times, the weight of the harvested frill lettuce was increased. ..
  • test was conducted in the same manner as in Test Group 3-1 except that the addition amount of glutathione was changed to 5.0 ml per 1 L of the nutrient solution once a day for 3 to 9 to 11 days from the day on which the frill lettuce germinated. I went. As a result, no sales-increasing effect of frill lettuce was observed.

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Abstract

The present invention provides a leaf vegetable production method having excellent production efficiency. In more detail, provided is a leaf vegetable production method comprising a step of applying, at least in a seedling-growing period from the day sprouting has occurred to the twelfth day after sprouting, oxidized glutathione on the underground portion of a leaf vegetable.

Description

葉物野菜の生産方法Production method of leafy vegetables 関連出願の参照Reference to related applications
　本特許出願は、２０１８年１１月２０日に出願された日本国特許出願２０１８－２１７０７３号に基づく優先権の主張を伴うものであり、かかる先の特許出願における全開示内容は、引用することにより本明細書の一部とされる。 This patent application is accompanied by a claim of priority based on Japanese Patent Application No. 2018-217073 filed on November 20, 2018, and all the disclosures in the above patent application are incorporated by reference. It is made a part of this specification.
　本発明は、葉物野菜の生産方法に関する。 The present invention relates to a method for producing leafy vegetables.
　グルタチオンは、Ｌ－システイン、Ｌ－グルタミン酸、グリシンの３種のアミノ酸からなるペプチドであり、人体だけではなく、他の動物、植物、微生物などの多くの生物生体内に存在し、活性酸素の除去作用、過酸化物の解毒作用等の機能が知られている。 Glutathione is a peptide consisting of three amino acids, L-cysteine, L-glutamic acid, and glycine, which exists not only in the human body, but in many living organisms such as other animals, plants, and microorganisms, and removes active oxygen. Functions such as action and detoxification of peroxide are known.
　グルタチオンは、Ｌ－システイン、Ｌ－グルタミン酸、グリシンの３種のアミノ酸からなるペプチド１分子単独で存在する還元型グルタチオンと、還元型グルタチオンの２分子がジスルフィド結合を介して結合した酸化型グルタチオンの、いずれかの形態で存在する。 Glutathione is composed of reduced glutathione, which is present as a single peptide molecule consisting of three amino acids, L-cysteine, L-glutamic acid, and glycine, and oxidized glutathione, in which two molecules of reduced glutathione are bound via a disulfide bond. It exists in either form.
　酸化型グルタチオンは、植物生長促進の機能を有することから、肥料分野において有用であることが知られている。例えば、酸化型グルタチオンを植物に投与することにより、植物の種子または花の数等が増加することが報告されている（特許文献１）。また、酸化型グルタチオンを着蕾期の塊茎植物等に投与することにより、塊茎等の生長が促進されたことが報告されている（特許文献２）。さらに、酸化型グルタチオンをリーフレタスに投与した際に、生長と亜鉛含有量の変化が観察されたことが報告されている（特許文献３）。 Oxidized glutathione is known to be useful in the fertilizer field because it has a function of promoting plant growth. For example, it has been reported that the number of seeds or flowers of a plant is increased by administering a plant with oxidized glutathione (Patent Document 1). Further, it has been reported that the growth of tubers and the like was promoted by administering oxidized glutathione to tubers and the like in the bud stage (Patent Document 2). Furthermore, it has been reported that changes in growth and zinc content were observed when oxidized glutathione was administered to leaf lettuce (Patent Document 3).
国際公開第２００８／０７２６０２号International Publication No. 2008/072602 特開２０１８－１１５１１６号公報Japanese Patent Laid-Open No. 2018-115116 特開２０１７－６３６３２号公報JP, 2017-63632, A
　本発明者らは、葉物野菜の生産における酸化型グルタチオンの施用時期を鋭意検討した結果、発芽した日から発芽後１２日目に至るまでの育苗期間内に酸化型グルタチオンを施用すると、生産される葉物野菜の重量が顕著に増加することを見出した。本発明はこの知見に基づくものである。 As a result of diligent examination of the application timing of oxidized glutathione in the production of leafy vegetables, the present inventors produced it when the oxidized glutathione was applied within the seedling raising period from the day of germination to the 12th day after germination. We found that the weight of leafy vegetables significantly increased. The present invention is based on this finding.
　従って、本発明は、生産効率の優れた葉物野菜の生産方法を提供する。 Therefore, the present invention provides a method for producing leafy vegetables having excellent production efficiency.
　本発明によれば、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜を生産する方法が提供される。 According to the present invention, leaf vegetables are produced, which comprises a step of applying oxidized glutathione to the underground part of leaf vegetables at least within the seedling raising period from the day of germination to the 12th day after germination. A method is provided.
　さらに、本発明によれば、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜の収穫量を増加させる方法が提供される。 Furthermore, according to the present invention, at least within the seedling raising period from the day of germination to the 12th day after germination, the step of applying oxidized glutathione to the underground portion of the leaf vegetables is used. A method of increasing yield is provided.
　さらに、本発明によれば、葉物野菜の収穫量を増加させるための組成物であって、酸化型グルタチオンを含んでなり、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に酸化型グルタチオンを施用するための組成物が提供される。 Furthermore, according to the present invention, a composition for increasing the yield of leafy vegetables, comprising oxidized glutathione, at least within the seedling raising period from the day of germination to the 12th day after germination. In, there is provided a composition for applying oxidized glutathione to the underground part of leafy vegetables.
　本発明によれば、生産される葉物野菜の重量が増加する点で有利である。また、本発明によれば、生産される葉物野菜の最終重量を同一にした場合に必要な生産（栽培）日数を低減化することができる。 According to the present invention, it is advantageous in that the weight of leaf vegetables produced is increased. Further, according to the present invention, it is possible to reduce the number of production (cultivation) days required when the final weight of leaf vegetables produced is the same.
発明の具体的説明Detailed explanation of the invention
１．葉物野菜を生産する方法
（１）葉物野菜の地下部への酸化型グルタチオンの施用
　本明細書において「酸化型グルタチオン」とは、還元型グルタチオン（Ｎ－（Ｎ－γ－Ｌ－グルタミル－Ｌ－システイニル）グリシン）の２分子がジスルフィド結合を介して結合した化合物である。本発明においては、酸化型グルタチオンには、他の物質と結合しておらず、イオン化していない遊離体、酸化型グルタチオンと酸または塩基とで形成される塩、これらの水和物、これらの混合物等の各種形態が包含され得る。 1. How to produce leafy vegetables
(1) Application of oxidized glutathione to the underground part of leafy vegetables In the present specification, “oxidized glutathione” means reduced glutathione (N- (N-γ-L-glutamyl-L-cysteinyl) glycine). It is a compound in which two molecules are bound via a disulfide bond. In the present invention, oxidized glutathione is not bound to other substances and is not an ionized educt, a salt formed with oxidized glutathione and an acid or a base, a hydrate of these, Various forms such as a mixture may be included.
　上述の酸化型グルタチオンの塩としては、アンモニウム塩、カルシウム塩、マグネシウム塩、ナトリウム塩、リチウム塩等の肥料として許容される塩であれば特に限定されない。酸化型グルタチオンの塩は、好ましくはアンモニウム塩、カルシウム塩及びマグネシウム塩から選択される１種以上の塩である。 The above-mentioned oxidized glutathione salt is not particularly limited as long as it is a salt acceptable as a fertilizer such as ammonium salt, calcium salt, magnesium salt, sodium salt, lithium salt and the like. The salt of oxidized glutathione is preferably one or more salts selected from ammonium salts, calcium salts and magnesium salts.
　本発明の方法で生産される葉物野菜は、主に葉の部分を食用とする野菜であれば、その種類を問わない。本発明において生産される葉物野菜の例としては、フリルレタス、ロメインレタス、バタビアレタス、ロロビオンダ、ロロロッサ、バターヘッドレタス、またはオークリーフレタス等のリーフレタス；結球レタス；からし菜（わさび菜）、ミズナ、葉ダイコン、白菜非結球品種、白菜結球性ミニ品種、非結球性キャベツなどのアブラナ科の葉菜、フダンソウ（スイスチャード）、ホウレンソウ（主にサラダ用品種が好ましい）、またはシュンギク等のその他の葉物野菜等が挙げられる。これらの中で、フリルレタス、ロメインレタス、バタビアレタス、ロロビオンダ、ロロロッサ、バターヘッドレタス、またはオークリーフレタス等のリーフレタスが好ましい。葉物野菜の生産に用いられる葉物野菜の種子または発芽直後の苗は、当業者に公知の方法で入手することができる。 The leafy vegetables produced by the method of the present invention may be of any type as long as they are vegetables whose leaf portion is mainly edible. Examples of leafy vegetables produced in the present invention include ruffle lettuce, romaine lettuce, batavia lettuce, lorobionda, lolorossa, butterhead lettuce, leaf lettuce such as oakley lettuce; headlet lettuce; mustard vegetable (wasabi vegetable), Mizuna , Leaf radish, Chinese cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage and other cruciferous leafy vegetables, chard (Swiss chard), spinach (mainly for salad variety), or other such as chrysanthemum Examples include leafy vegetables. Of these, leaf lettuce such as frill lettuce, romaine lettuce, Batavia lettuce, lorobinonda, lolorossa, butterhead lettuce, or oakley lettuce is preferable. The seeds of leafy vegetables or seedlings immediately after germination used for the production of leafy vegetables can be obtained by methods known to those skilled in the art.
　本明細書において「葉物野菜の地下部」とは、葉物野菜の植物体のうち、土壌または培地等に接しており、空気中に露出されていない部分をいう。この地下部には、葉物野菜の生長に必要な栄養分および水分を吸収する機能がある。本発明において酸化型グルタチオンは地下部から吸収される。本発明の好ましい一つの実施態様では、葉物野菜の地下部は根を含むものとされる。 In the present specification, the “underground part of leafy vegetables” refers to a part of a plant body of leafy vegetables that is in contact with soil or a medium and is not exposed to the air. This underground part has a function of absorbing nutrients and water necessary for growing leafy vegetables. In the present invention, oxidized glutathione is absorbed from underground. In one preferred embodiment of the present invention, the subterranean part of the leafy vegetables comprises roots.
　本明細書において「葉物野菜の地上部」とは、葉物野菜の植物体のうち、土壌または培地等に接しておらず、空気中に露出されている部分をいう。この地上部には、葉物野菜の生長に必要な光合成を行う機能がある。また、この地上部は生産（栽培）された後、食用に供される部分である。本発明の好ましい一つの実施態様では、葉物野菜の地上部は葉を含むものとされる。 In the present specification, the “above-ground portion of leafy vegetables” refers to a portion of a plant body of leafy vegetables which is not in contact with soil or a medium and is exposed to the air. This aerial part has the function of performing photosynthesis necessary for the growth of leafy vegetables. The above-ground part is a part that is produced (cultivated) and then used for food. In one preferred embodiment of the present invention, the above-ground part of the leafy vegetables comprises leaves.
　本発明の方法において、酸化型グルタチオンは地下部に施用される。施用される酸化型グルタチオンの状態は、葉物野菜の地下部を経由して吸収されうる状態のものであればいずれの状態でもよい。本発明の方法で施用されうる酸化型グルタチオンの状態は、例えば、酸化型グルタチオンを水もしくは水溶性溶媒（メタノール、エタノール等）またはこれらの混合溶媒に溶解した酸化型グルタチオン含有溶液であってもよい。あるいは、酸化型グルタチオンの単体の、もしくは他の化合物との混合物である、粉末もしくは顆粒等の酸化型グルタチオン含有固形物であってもよい。 In the method of the present invention, oxidized glutathione is applied underground. The oxidized glutathione applied may be in any state as long as it can be absorbed via the underground part of the leafy vegetables. The state of oxidized glutathione that can be applied by the method of the present invention may be, for example, an oxidized glutathione-containing solution obtained by dissolving oxidized glutathione in water or a water-soluble solvent (methanol, ethanol, etc.) or a mixed solvent thereof. .. Alternatively, it may be a solid substance containing oxidized glutathione, such as powder or granules, which is a simple substance of oxidized glutathione or a mixture with other compounds.
　本発明の方法で施用される酸化型グルタチオンが酸化型グルタチオン含有溶液の状態である場合、この溶液は、酸化型グルタチオン以外に、水、メタノール、エタノール等の水溶性溶媒等の溶媒を含むことができる。さらに、この溶液は、界面活性剤（直鎖アルキルベンゼンスルホン酸塩等）、分散安定化剤（カルボキシメチルセルロース、その塩等）、増粘剤、酸化防止剤等の成分を含んでいてもよい。 When the oxidized glutathione applied by the method of the present invention is in the state of an oxidized glutathione-containing solution, this solution may contain a solvent such as a water-soluble solvent such as water, methanol or ethanol in addition to the oxidized glutathione. it can. Furthermore, this solution may contain components such as a surfactant (straight chain alkylbenzene sulfonate, etc.), a dispersion stabilizer (carboxymethyl cellulose, its salt, etc.), a thickener, an antioxidant and the like.
　本発明の方法で施用される酸化型グルタチオンが酸化型グルタチオン含有溶液の状態である場合は、該溶液中の酸化型グルタチオンの含有濃度は、地下部を経由して葉物野菜に吸収されうる濃度のものであれば、特に限定されない。例えば、酸化型グルタチオンの遊離体換算で、２０００ｐｐｍ（ｗ／ｖ）～１００００ｐｐｍ（ｗ／ｖ）が好ましく、４０００ｐｐｍ（ｗ／ｖ）～８０００ｐｐｍ（ｗ／ｖ）が好ましく、５０００ｐｐｍ（ｗ／ｖ）～７０００ｐｐｍ（ｗ／ｖ）がより好ましく、６０００ｐｐｍ（ｗ／ｖ）がさらに好ましい。 When the oxidized glutathione applied by the method of the present invention is in the state of an oxidized glutathione-containing solution, the content concentration of the oxidized glutathione in the solution is a concentration that can be absorbed by leaf vegetables via the underground part. It is not particularly limited as long as it is one. For example, in terms of free form of oxidized glutathione, 2000 ppm (w / v) to 10000 ppm (w / v) is preferable, 4000 ppm (w / v) to 8000 ppm (w / v) is preferable, and 5000 ppm (w / v) to 7000 ppm (w / v) is more preferable, and 6000 ppm (w / v) is further preferable.
　本発明のより好ましい実施態様では、施用される酸化型グルタチオンは水溶液の状態である。 In a more preferred embodiment of the present invention, the applied oxidized glutathione is in the form of an aqueous solution.
　本発明の方法における酸化型グルタチオンの状態が酸化型グルタチオン含有固形物である場合、この固形物は、界面活性剤（直鎖アルキルベンゼンスルホン酸塩、アルキルナフタレンスルホン酸塩、ラウリル硫酸塩、ヒマシ油カリ石けん等）、分散安定化剤（カルボキシメチルセルロース、その塩、等）、賦形剤（乳糖等）、崩壊剤、増粘剤、酸化防止剤等の成分を含んでいてもよい。該固形物中での酸化型グルタチオンの含有濃度は、葉物野菜の地下部から吸収されうる濃度のものであればよく、特に限定されないが、施用される時点において、酸化型グルタチオンの遊離体換算で例えば０．０１～５０質量％となる濃度であることが好ましい。 When the oxidized glutathione state in the method of the present invention is an oxidized glutathione-containing solid, the solid is a surfactant (straight chain alkylbenzene sulfonate, alkylnaphthalene sulfonate, lauryl sulfate, castor oil potassium salt). Soaps, etc.), dispersion stabilizers (carboxymethyl cellulose, salts thereof, etc.), excipients (lactose, etc.), disintegrants, thickeners, antioxidants and other components may be included. The concentration of oxidized glutathione contained in the solid matter is not particularly limited as long as it is a concentration that can be absorbed from the underground portion of leafy vegetables, but at the time of application, it is converted into oxidized glutathione free form. It is preferable that the concentration is, for example, 0.01 to 50% by mass.
　本発明の好ましい実施態様では、上記酸化型グルタチオンの施用は、酸化型グルタチオンの吸収効率の観点から、酸化型グルタチオン含有培地中に葉物野菜の地下部を浸漬することにより実施することができる。また、同様の観点から、上記培地は肥料成分および酸化型グルタチオンの水溶液の状態である養液であることがより好ましい。 In a preferred embodiment of the present invention, the above-mentioned application of oxidized glutathione can be carried out by immersing the underground portion of leafy vegetables in an oxidized glutathione-containing medium from the viewpoint of the absorption efficiency of oxidized glutathione. From the same viewpoint, it is more preferable that the medium is a nutrient solution in the state of an aqueous solution of the fertilizer component and oxidized glutathione.
（２）葉物野菜の発芽した日から発芽後１２日目に至るまでの育苗期間内における酸化型グルタチオンの施用
　本発明の方法では、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用することにより、生産される葉物野菜の重量を増加させることができる。ここで、「発芽した日から発芽後１２日目に至るまで」は、「発芽した日から発芽後１１日目の終わりまで」を意味する。 (2) Application of oxidized glutathione within the period for raising seedlings from the day of germination of leafy vegetables to the 12th day after germination In the method of the present invention, at least seedling raising from the day of germination until the 12th day after germination By applying oxidized glutathione to the underground part of the leafy vegetables within the period, the weight of the leafy vegetables produced can be increased. Here, "from the day of germination to 12th day after germination" means "from the day of germination to the end of 11th day after germination".
　従来から、酸化型グルタチオンが植物における種々の生長促進作用を有することは知られていたものの、その作用は酸化型グルタチオンの施用時期に大きく依存していた。本発明において、葉物野菜に対する酸化型グルタチオンの施用時期を検討したところ、発芽した日から発芽後１２日目に至るまでの育苗期間内に酸化型グルタチオンを施用すると、生産される葉物野菜の重量が顕著に増加することが判明した。特定の理論に拘泥するものではないが、発芽後光合成作用が始まっており、本格的に生長する時期の準備段階にあたる育苗期間内に酸化型グルタチオンを施用すると、生長促進作用の一つである重量増加促進作用が効率的に誘導され、葉物野菜の収穫重量の増加につながるものと考えられる。 Previously, it was known that oxidized glutathione had various growth promoting effects in plants, but the effect was largely dependent on the application period of oxidized glutathione. In the present invention, when the application time of oxidized glutathione to leafy vegetables was examined, and when oxidized glutathione was applied within the seedling raising period from the day of germination to the 12th day after germination, leafy vegetables produced were It was found that the weight increased significantly. Although not limited to any particular theory, photosynthesis has begun after germination, and if oxidized glutathione is applied during the seedling raising period, which is the preparatory stage of full-scale growth, it is one of the growth promoting effects. It is considered that the increase promoting effect is efficiently induced, which leads to an increase in the harvest weight of leafy vegetables.
　本発明における育苗期間は、葉物野菜が発芽した日から発芽後１２日目に至るまでとされる。この発芽後１２日目には、葉物野菜の一株当たりの重量（地上部および地下部の重量の総和）が０．２～０．５ｇに到達しており、本葉が展開しきった状態となっている。　 The seedling raising period in the present invention is from the day when leafy vegetables germinate to the 12th day after germination. On the 12th day after germination, the weight per leaf plant (the total weight of the above-ground portion and the below-ground portion) reached 0.2 to 0.5 g, and the true leaves were fully developed. Has become. ‥
　本発明の好ましい実施態様では、上記育苗期間内における酸化型グルタチオンの総施用量は、１株当たり０．３７５ｍｇ（以下「ｍｇ／株」と記述する）～１．５ｍｇ／株とされ、より好ましくは０．３７５ｍｇ／株～１．１２５ｍｇ／株、さらに好ましくは０．３７５ｍｇ／株～０．７５ｍｇ／株、さらにより好ましくは０．５６２５ｍｇ／株とされる。 In a preferred embodiment of the present invention, the total applied amount of oxidized glutathione within the above-mentioned seedling raising period is 0.375 mg (hereinafter referred to as “mg / strain”) to 1.5 mg / strain, and more preferably Is 0.375 mg / strain to 1.125 mg / strain, more preferably 0.375 mg / strain to 0.75 mg / strain, and even more preferably 0.5625 mg / strain.
　本発明の好ましい実施態様では、上記育苗期間内における酸化型グルタチオンの施用期間は１～５日とされ、好ましくは２～５日、より好ましくは３～５日、さらに好ましくは３～４日、さらにより好ましくは３日とされる。本発明のより好ましい実施態様では、施用期間は、発芽した日から７日目以降の期間とされる。本発明のさらに好ましい実施態様では、上記施用期間中は、毎日、酸化型グルタチオンが施用される。本発明のさらに好ましい実施態様では、発芽した日から９日目から１１日目までの毎日、酸化型グルタチオンが施用される。 In a preferred embodiment of the present invention, the period of application of oxidized glutathione within the seedling raising period is 1 to 5 days, preferably 2 to 5 days, more preferably 3 to 5 days, still more preferably 3 to 4 days. Even more preferably, it is 3 days. In a more preferred embodiment of the present invention, the application period is the period from the 7th day after germination to the 7th day or later. In a further preferred embodiment of the present invention, oxidized glutathione is applied daily during the above application period. In a further preferred embodiment of the present invention, oxidized glutathione is applied every day from the day of germination to day 9 to day 11.
　本発明の好ましい実施態様では、上記育苗期間内における酸化型グルタチオンの１日当たりの施用量は、１株当たり０．１２５ｍｇ／株～０．５ｍｇ／株とされ、より好ましくは０．１２５ｍｇ／株～０．３７５ｍｇ／株、さらに好ましくは０．１２５ｍｇ／株～０．２５ｍｇ／株、さらにより好ましくは０．１８７５ｍｇ／株とされる。 In a preferred embodiment of the present invention, the daily application rate of oxidized glutathione within the above-mentioned seedling raising period is 0.125 mg / strain to 0.5 mg / strain, more preferably 0.125 mg / strain to The amount is 0.375 mg / strain, more preferably 0.125 mg / strain to 0.25 mg / strain, and even more preferably 0.1875 mg / strain.
（３）発芽した日から１２日目以降の生育期間内における酸化型グルタチオンの施用
　本発明の方法において、酸化型グルタチオンを上記育苗期間内に施用することに加え、発芽した日から１２日目以降の生育期間内にさらに酸化型グルタチオンを施用することができる。これにより生産される葉物野菜の重量を増加させることができる。 (3) Application of oxidized glutathione within the growth period from the day of germination to day 12 or later In the method of the present invention, in addition to the application of oxidized glutathione within the period of seedling raising, 12 days after germination Oxidized glutathione can be further applied within the growing period of. As a result, the weight of leafy vegetables produced can be increased.
　本発明の好ましい実施態様では、上記生育期間内における酸化型グルタチオンの総施用量は、１株当たり０．２６３ｍｇ／株～２．６３ｍｇ／株とされ、より好ましくは０．２６３ｍｇ／株～１．９８ｍｇ／株、さらに好ましくは０．６７ｍｇ／株～１．９８ｍｇ／株、さらにより好ましくは１．３１ｍｇ／株とされる。 In a preferred embodiment of the present invention, the total application amount of oxidized glutathione within the growth period is 0.263 mg / strain to 2.63 mg / strain, more preferably 0.263 mg / strain to 1. The amount is 98 mg / strain, more preferably 0.67 mg / strain to 1.98 mg / strain, and even more preferably 1.31 mg / strain.
　本発明の好ましい実施態様では、上記生育期間内における酸化型グルタチオンの施用期間は、葉物野菜が発芽した日から数えて１２日目から葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日までとされる。 In a preferred embodiment of the present invention, the application period of oxidized glutathione within the above-mentioned growth period is from the 12th day from the day when the leafy vegetables germinate, from the weight of the leafy vegetables (the total weight of the above-ground portion and the underground portion). ) Reaches 28 to 40 g per share.
　本発明の好ましい実施態様では、酸化型グルタチオンの施用は、上記生育期間内において、１日で、または複数日に分けて実施することができる。 In a preferred embodiment of the present invention, the application of oxidized glutathione can be carried out in one day or divided into a plurality of days within the above growth period.
　本発明のより好ましい実施態様では、上記生育期間内において、酸化型グルタチオンの施用は複数日に分けて実施される。 In a more preferred embodiment of the present invention, the application of oxidized glutathione is carried out for a plurality of days within the above growth period.
　本発明のさらに好ましい実施態様では、上記生育期間内において、酸化型グルタチオンの施用は３日に分けて実施される。 In a further preferred embodiment of the present invention, the application of oxidized glutathione is carried out every 3 days within the above growth period.
　本発明のよりさらに好ましい実施態様では、上記生育期間内において、発芽した日から１２日目、葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２．９～５．３ｇに到達する日、および葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日に、酸化型グルタチオンが施用される。 In a further preferred embodiment of the present invention, within the above growth period, on the 12th day from the day of germination, the weight of leafy vegetables (the sum of the weights of the above-ground portion and the below-ground portion) is 2.9 to 5. Oxidized glutathione is applied on the day when it reaches 3 g, and on the day when the weight of leafy vegetables (the sum of the weights of the above-ground part and the underground part) reaches 28 to 40 g per strain.
　本発明の好ましい実施態様では、上記生育期間内における酸化型グルタチオンの１日当たりの施用量は、葉物野菜１株当たり０．０４６ｍｇ／株～１．５ｍｇ／株とされ、より好ましくは０．０４６ｍｇ／株～１．１３ｍｇ／株、さらに好ましくは０．１２ｍｇ／株～１．１３ｍｇ／株、さらにより好ましくは０．２３ｍｇ／株～０．７５ｍｇ／株とされる。 In a preferred embodiment of the present invention, the daily application rate of oxidized glutathione during the above-mentioned growth period is 0.046 mg / strain to 1.5 mg / strain per leaf vegetable, more preferably 0.046 mg. / Strain to 1.13 mg / strain, more preferably 0.12 mg / strain to 1.13 mg / strain, and even more preferably 0.23 mg / strain to 0.75 mg / strain.
　本発明のより好ましい実施態様では、上記発芽した日から１２日目における酸化型グルタチオンの１日当たりの施用量は、葉物野菜１株当たり０．０４６ｍｇ／株～０．４６ｍｇ／株とされ、より好ましくは０．０４６ｍｇ／株～０．３５ｍｇ／株、さらに好ましくは０．１２ｍｇ／株～０．３５ｍｇ／株、さらにより好ましくは０．２３ｍｇ／株とされる。 In a more preferred embodiment of the present invention, the daily application rate of oxidized glutathione on the 12th day from the germination day is 0.046 mg / strain to 0.46 mg / strain per leaf vegetable, and The amount is preferably 0.046 mg / strain to 0.35 mg / strain, more preferably 0.12 mg / strain to 0.35 mg / strain, and even more preferably 0.23 mg / strain.
　本発明のより好ましい実施態様では、上記葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２．９～５．３ｇに到達する日における酸化型グルタチオンの１日当たりの施用量を葉物野菜１株当たり０．０６７ｍｇ／株～０．６７ｍｇ／株とされ、より好ましくは０．０６７ｍｇ／株～０．５ｍｇ／株、さらに好ましくは０．１７ｍｇ／株～０．５ｍｇ／株、さらにより好ましくは０．３３ｍｇ／株とされる。 In a more preferred embodiment of the present invention, the amount of oxidized glutathione applied per day on the day when the weight of the leafy vegetables (sum of the weight of the above-ground portion and the amount of the below-ground portion) reaches 2.9 to 5.3 g per strain. The dose is 0.067 mg / strain to 0.67 mg / strain per leaf vegetable, more preferably 0.067 mg / strain to 0.5 mg / strain, further preferably 0.17 mg / strain to 0.5 mg / strain. The strain, and even more preferably 0.33 mg / strain.
　本発明のより好ましい実施態様では、上記葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日における酸化型グルタチオンの１日当たりの施用量を葉物野菜１株当たり０．１５ｍｇ／株～１．５ｍｇ／株とされ、より好ましくは０．１５ｍｇ／株～１．１３ｍｇ／株、さらに好ましくは０．３８ｍｇ／株～１．１３ｍｇ／株、さらにより好ましくは０．７５ｍｇ／株とされる。 In a more preferred embodiment of the present invention, the daily application amount of oxidized glutathione on the day when the weight of the leafy vegetables (sum of the weights of the above-ground portion and the below-ground portion) reaches 28 to 40 g per strain, 0.15 mg / strain to 1.5 mg / strain per vegetable, more preferably 0.15 mg / strain to 1.13 mg / strain, even more preferably 0.38 mg / strain to 1.13 mg / strain, and even more The amount is preferably 0.75 mg / strain.
　本発明のさらに好ましい実施態様では、酸化型のグルタチオンを上記育苗期間内に施用することに加え、上記生育期間内にさらに施用する場合において、上記育苗期間内における酸化型グルタチオンの総施用量を１株当たり０．３７５ｍｇ／株～１．５ｍｇ／株とされ、より好ましくは０．５６２５ｍｇ／株～１．５ｍｇ／株、さらに好ましくは０．７５ｍｇ／株～１．５ｍｇ／株、さらにより好ましくは１．１２５ｍｇ／株とされる。 In a further preferred embodiment of the present invention, in addition to the application of oxidized glutathione within the seedling raising period, in the case of further application within the growing period, the total application amount of oxidized glutathione within the seedling raising period is 1 It is set to 0.375 mg / strain to 1.5 mg / strain, more preferably 0.5625 mg / strain to 1.5 mg / strain, even more preferably 0.75 mg / strain to 1.5 mg / strain, and even more preferably It will be 1.125 mg / strain.
　本発明のさらに好ましい実施態様では、酸化型のグルタチオンを上記育苗期間内に施用することに加え、上記生育期間内にさらに施用する場合において、上記育苗期間内における酸化型グルタチオンの１日当たりの施用量を１株当たり０．１２５ｍｇ／株～０．５ｍｇ／株とされ、より好ましくは０．１８７５ｍｇ／株～０．５ｍｇ／株、さらに好ましくは０．２５ｍｇ／株～０．５ｍｇ／株、さらにより好ましくは０．３７５ｍｇ／株とされる。 In a further preferred embodiment of the present invention, in addition to the application of oxidized glutathione within the seedling raising period, in the case of further application within the growing period, the daily application amount of oxidized glutathione within the seedling raising period. Is 0.125 mg / strain to 0.5 mg / strain, more preferably 0.1875 mg / strain to 0.5 mg / strain, even more preferably 0.25 mg / strain to 0.5 mg / strain, and even more It is preferably 0.375 mg / strain.
（４）その他の生産条件
　本発明における葉物野菜の生産方法は、露地栽培等の通常の開放環境下または閉鎖環境下のいずれにおいても実施することができる。本発明の方法による葉物野菜の生産にあたり、当業者であれば、これらの環境条件を適宜選択することができる。本発明の好ましい実施態様では、葉物野菜の生産方法は、閉鎖環境下において実施される。閉鎖環境下においては、外界の環境変化に左右されず一定の生産効率および品質を確保することができる。さらに、閉鎖環境には、温室などの半完全閉鎖系環境、または栽培環境を外部と完全に遮断して環境条件を厳密に管理する完全閉鎖系環境が含まれる。 (4) Other production conditions The method for producing leafy vegetables according to the present invention can be carried out either under an ordinary open environment such as open-air cultivation or under a closed environment. Those skilled in the art can appropriately select these environmental conditions for producing leafy vegetables by the method of the present invention. In a preferred embodiment of the present invention, the method for producing leafy vegetables is carried out in a closed environment. In a closed environment, constant production efficiency and quality can be secured without being affected by environmental changes in the external environment. Further, the closed environment includes a semi-fully closed environment such as a greenhouse, or a completely closed environment in which the cultivation environment is completely shielded from the outside and the environmental conditions are strictly controlled.
　本明細書において「培地」とは、葉物野菜の生産（栽培）に必要な栄養成分（窒素、リン酸、カリ等）および水分を葉物野菜に保持供給する機能を有するものをいい、液体状であると固形状であるとを問わない。例えば、開放環境や半完全閉鎖系環境において使用される土壌や固形担体、半完全閉鎖環境や完全閉鎖系環境において使用される養液等が挙げられる。本発明の好ましい実施態様では、葉物野菜の地下部は培地中に浸漬されている。　 In the present specification, the "medium" refers to a medium having a function of retaining and supplying nutrient components (nitrogen, phosphoric acid, potassium, etc.) and water necessary for production (cultivation) of leaf vegetables to leaf vegetables. It does not matter whether it is solid or solid. Examples thereof include soil and solid carriers used in an open environment or a semi-closed environment, and nutrient solutions used in a semi-closed environment or a completely closed environment. In a preferred embodiment of the present invention, the underground part of the leafy vegetables is immersed in the medium. ‥
　本発明のより好ましい実施態様では、培地は栄養成分を水に溶解した養液である。養液を用いた葉物野菜の生産は、一般に水耕栽培と呼ばれ、当業者に公知の方法により実施することができる。水耕栽培において、養液中の各栄養成分の濃度を容易に制御することができる。また、養液中の栄養成分の濃度を一定に保持することもできる。本発明の方法における養液の栄養成分は、通常の葉物野菜の水耕栽培で使われている通常の配合割合の養液を使用することができる。本発明の方法において使用される養液は、例えば、葉物野菜の水耕栽培向けの多量必須元素および微量必須元素を含有してもよい。このうち、多量必須元素は、例えば、窒素、リン、カリウム、カルシウム、マグネシウム、硫黄の６元素である。また、微量必須元素は、例えば、鉄、マンガン、亜鉛、銅、モリブデン、ホウ素、塩素の７元素である。 In a more preferred embodiment of the present invention, the medium is a nutrient solution in which nutrient components are dissolved in water. The production of leafy vegetables using a nutrient solution is generally called hydroponics and can be carried out by a method known to those skilled in the art. In hydroponic culture, the concentration of each nutrient in the nutrient solution can be easily controlled. In addition, the concentration of nutrient components in the nutrient solution can be kept constant. As the nutrient component of the nutrient solution in the method of the present invention, a nutrient solution having a usual mixing ratio used in the hydroponics of ordinary leafy vegetables can be used. The nutrient solution used in the method of the present invention may contain, for example, a large amount of essential element and a small amount of essential element for hydroponics of leafy vegetables. Among these, the large amount of essential elements are, for example, 6 elements of nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur. Moreover, the trace essential elements are, for example, seven elements of iron, manganese, zinc, copper, molybdenum, boron, and chlorine.
　本発明のより好ましい実施態様では、葉物野菜の生産方法は、完全閉鎖系環境下において実施することができる。完全閉鎖系環境下においては、生産された葉物野菜が均一の品質を有するといった有利な点がある。 In a more preferred embodiment of the present invention, the method for producing leafy vegetables can be carried out under a completely closed environment. In a completely closed environment, there is an advantage that the leaf vegetables produced have a uniform quality.
　本発明の好ましい実施態様において、本発明の方法は植物工場で実施することができる。ここで、植物工場とは、植物の生育環境を制御して栽培を行う施設のうち、栽培環境および生育のモニタリングを基礎として環境制御と生育予測を行うことにより、植物の周年生産を可能とする栽培施設をいう。本発明の方法を植物工場において実施することにより、葉物野菜に対する酸化型グルタチオンの施用量や施用タイミングを定量的かつ周期的に制御できることで、一定品質の葉物野菜を大量に生産することができる。 In a preferred embodiment of the present invention, the method of the present invention can be carried out in a plant factory. Here, the plant factory enables the year-round production of plants by performing environmental control and growth prediction based on the monitoring of the cultivation environment and growth among the facilities that control and grow the growth environment of plants. A cultivation facility. By carrying out the method of the present invention in a plant factory, it is possible to quantitatively and cyclically control the application amount and application timing of oxidized glutathione to leafy vegetables, thereby producing a large amount of leafy vegetables of constant quality. it can.
２.本発明の方法により生産された葉物野菜
　本発明の別の態様として、本発明の方法により生産された葉物野菜が提供される。本発明の方法により生産された葉物野菜は、酸化型グルタチオンの吸収によって惹起された当該葉物野菜中の生理状態を遺伝子発現分析または代謝産物のメタボローム解析等によって決定し、その結果を比較することにより、酸化型グルタチオンを施用しない葉物野菜と区別することができる。また、本発明の方法により生産された葉物野菜は、当該葉物野菜の酸化型グルタチオンの吸収によって惹起された呈味の変化を調べることにより、酸化型グルタチオンを施用しない葉物野菜と区別することができる。 2. Leafy Vegetable Produced by the Method of the Present Invention As another aspect of the present invention, a leafy vegetable produced by the method of the present invention is provided. Leaf vegetables produced by the method of the present invention, the physiological state in the leaf vegetables caused by absorption of oxidized glutathione is determined by gene expression analysis or metabolome analysis of metabolites, and the results are compared. Thus, it can be distinguished from leafy vegetables to which oxidized glutathione is not applied. The leafy vegetables produced by the method of the present invention are distinguished from leafy vegetables to which oxidized glutathione is not applied by examining the change in taste caused by absorption of oxidized glutathione in the leafy vegetables. be able to.
３．葉物野菜の収穫量を増加させる方法
　本発明の別の態様として、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜の収穫量を増加させる方法が提供される。 3. Method for increasing yield of leafy vegetables As another aspect of the present invention, oxidized glutathione is applied to the underground part of leafy vegetables at least within the seedling raising period from the day of germination to the 12th day after germination. Provided is a method of increasing the yield of leafy vegetables, the method comprising the steps of:
　本発明の方法において、葉物野菜の収穫量の増加は、葉物野菜の地上部の重量の増加であることが好ましい。 In the method of the present invention, the increase in the yield of leafy vegetables is preferably an increase in the weight of the above-ground portion of the leafy vegetables.
　本発明の方法において、葉物野菜の地上部の重量の増加率が５％以上であることが好ましく、６％以上であることがより好ましく、８％以上であることがさらに好ましく、１０％以上であることがさらにより好ましく、１２％以上であることが特に好ましい。ここで、酸化型グルタチオンを施用して生産した葉物野菜の地上部の重量の平均値をＡとし、酸化型グルタチオンを施用しない以外は同じ条件で生産した葉物野菜の地上部の重量の平均値をＢとすると、増加率は、以下の式で表される。
　　［数１］
増加率＝（Ａ－Ｂ）／Ｂ×１００ In the method of the present invention, the rate of increase in the weight of the above-ground portion of leafy vegetables is preferably 5% or more, more preferably 6% or more, further preferably 8% or more, more preferably 10% or more. Is more preferable, and 12% or more is particularly preferable. Here, the average value of the above-ground weight of leaf vegetables produced by applying oxidized glutathione is A, and the average above-ground weight of leaf vegetables produced under the same conditions except that oxidized glutathione is not applied. When the value is B, the increase rate is expressed by the following formula.
[Equation 1]
Rate of increase = (AB) / B × 100
　本発明の方法によれば、同じ日数で栽培した場合に生産される葉物野菜の重量が増加する点で有利である。また、本発明によれば、生産される葉物野菜の最終重量を同一にした場合に必要な生産（栽培）日数を減らすことができる。 The method of the present invention is advantageous in that the weight of leafy vegetables produced when grown for the same number of days increases. Further, according to the present invention, it is possible to reduce the number of production (cultivation) days required when the final weight of leaf vegetables produced is the same.
４．葉物野菜の収穫量を増加させるための組成物
　本発明の別の態様として、葉物野菜の収穫量を増加させるための組成物であって、酸化型グルタチオンを含んでなり、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に酸化型グルタチオンを施用するための組成物が提供される。 4. Composition for increasing the yield of leafy vegetables Another aspect of the present invention is a composition for increasing the yield of leafy vegetables, comprising oxidized glutathione, at least on the day of germination. A composition for applying oxidized glutathione to the underground part of leafy vegetables within the period from seedling to the 12th day after germination.
　本発明の好ましい一つの実施態様では、上記葉物野菜の収穫量を増加させるための組成物には、葉物野菜用肥料組成物または葉物野菜用生長促進剤が含まれる。 In a preferred embodiment of the present invention, the composition for increasing the yield of leafy vegetables comprises a fertilizer composition for leafy vegetables or a growth promoter for leafy vegetables.
　本発明の葉物野菜用肥料組成物は、酸化型グルタチオンを含んでなるものである。また、上述した酸化型グルタチオンの作用を損なわない限り、酸化型グルタチオンの他に、葉物野菜一般に用いられる肥料成分をさらに含んでいてもよい。ここで、肥料成分とは、窒素、リン、カリウム、ケイ素、マグネシウム、カルシウム、マンガン、ホウ素、鉄等の葉物野菜に利用される元素を指す。本発明の葉物野菜用肥料組成物における酸化型グルタチオンの他の肥料成分は、葉物野菜に利用可能な形態である、肥料成分元素を含有する有機化合物、無機化合物またはそれらの組合せを利用することができる。本発明に用いられる肥料成分としては、窒素、リン、カリウム、ケイ素、マグネシウム、カルシウム、マンガン、ホウ素および鉄からなる群から選択される少なくとも１種を少なくとも含むことが好ましく、窒素、リンおよびカリウムからなる群から選択される少なくとも１種を含んでなることがより好ましく、窒素、リンおよびカリウムの３種の組合せを少なくとも含むことがさらに好ましい。 The fertilizer composition for leafy vegetables of the present invention comprises oxidized glutathione. In addition to the oxidized glutathione, it may further contain a fertilizer component generally used for leafy vegetables, as long as the action of the oxidized glutathione described above is not impaired. Here, the fertilizer component refers to an element used for leafy vegetables such as nitrogen, phosphorus, potassium, silicon, magnesium, calcium, manganese, boron, and iron. The other fertilizer component of oxidized glutathione in the fertilizer composition for leaf vegetables of the present invention uses an organic compound containing a fertilizer component element, which is a form that can be used for leaf vegetables, an inorganic compound, or a combination thereof. be able to. The fertilizer component used in the present invention preferably contains at least one selected from the group consisting of nitrogen, phosphorus, potassium, silicon, magnesium, calcium, manganese, boron and iron. From nitrogen, phosphorus and potassium More preferably, it comprises at least one selected from the group consisting of, and it is even more preferred that it contains at least a combination of three kinds of nitrogen, phosphorus and potassium.
　本発明の葉物野菜用肥料組成物は、上記の葉物野菜の生産方法、あるいは葉物野菜の収穫量を増加させる方法において使用してもよい。 The fertilizer composition for leafy vegetables of the present invention may be used in the above-mentioned method for producing leafy vegetables or a method for increasing the yield of leafy vegetables.
　本発明の葉物野菜用生長促進剤は、酸化型グルタチオンを含んでなるものである。また、上述した酸化型グルタチオンの作用を損なわない限り、酸化型グルタチオンの他に、さらに、植物ホルモンおよび／または生長促進剤を含んでいてもよい。ここで、植物ホルモンには、例えば、ジベレリンが挙げられる。また、生長促進剤には、例えば、安息香酸、ニコチン酸、ニコチン酸アミド、ピペコリン酸等が挙げられる。 The growth promoter for leafy vegetables of the present invention comprises oxidized glutathione. In addition to the oxidized glutathione, a plant hormone and / or a growth promoter may be further contained, as long as the action of the oxidized glutathione described above is not impaired. Here, examples of the plant hormone include gibberellin. Further, examples of the growth promoting agent include benzoic acid, nicotinic acid, nicotinic acid amide, pipecolic acid and the like.
　本発明の生長促進剤は、上記の葉物野菜の生産方法、あるいは葉物野菜の収穫量を増加させる方法において使用してもよい。 The growth promoter of the present invention may be used in the method for producing leafy vegetables or the method for increasing the yield of leafy vegetables.
　また、一つの実施態様によれば、本発明には、以下の［１］～［２０］が包含される。［１］少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜を生産する方法。
［２］前記葉物野菜の地下部が根を含んでなる、［１］に記載の方法。
［３］前記育苗期間内における前記酸化型グルタチオンの総施用量が、１株当たり０．３７５～１．５ｍｇである、［１］または［２］に記載の方法。
［４］前記育苗期間内における酸化型グルタチオンの施用期間が、１～５日である、［１］～［３］のいずれかに記載の方法。
［５］前記施用期間が、発芽した日から７日目以降の期間である、［１］～［４］のいずれかに記載の方法。
［６］前記施用期間において酸化型グルタチオンを毎日施用する、［４］または［５］に記載の方法。
［７］前記育苗期間内における前記酸化型グルタチオンの１日当たりの施用量が、１株当たり０．１２５～０．５ｍｇである、［４］～［６］のいずれかに記載の方法。
［８］前記酸化型グルタチオンの施用が、酸化型グルタチオン含有培地中に葉物野菜の地下部を浸漬することを含んでなる、［１］～［７］のいずれかに記載の方法。
［９］前記酸化型グルタチオン含有培地が養液である、［８］に記載の方法。
［１０］発芽した日から１２日目以降の生育期間内にさらに酸化型グルタチオンを施用する、［１］～［９］のいずれかに記載の方法。
［１１］前記生育期間内における前記酸化型グルタチオンの総施用量が、１株当たり０．２６３～２．６３ｍｇである、［１０］に記載の方法。
［１２］前記生育期間内における酸化型グルタチオンの施用期間が、発芽した日から１２日目から葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日までである、［１０］または［１１］に記載の方法。
［１３］前記生育期間内において、酸化型グルタチオンの施用を１日でまたは複数日に分けて実施する、［１０］～［１２］のいずれかに記載の方法。
［１４］酸化型グルタチオンの施用を、発芽した日から１２日目、葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２．９～５．３ｇに到達する日、および葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日に実施する、［１０］～［１３］のいずれかに記載の方法。
［１５］前記生育期間内における前記酸化型グルタチオンの１日当たりの施用量が、１株当たり０．０４６～１．５ｍｇである、［１０］～［１４］のいずれかに記載の方法。
［１６］閉鎖環境下で行われる、［１］～［１５］のいずれかに記載の方法。
［１７］植物工場で行われる、［１］～［１６］のいずれかに記載の方法。
［１８］前記葉物野菜が、リーフレタス、結球レタス、アブラナ科の葉菜、からし菜（わさび菜）、ミズナ、葉ダイコン、白菜非結球品種、白菜結球性ミニ品種、非結球性キャベツ、フダンソウ（スイスチャード）、ホウレンソウおよびシュンギクからなる群から選択される、［１］～［１７］のいずれかに記載の方法。
［１９］少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜の収穫量を増加させる方法。
［２０］葉物野菜の収穫量を増加させるための組成物であって、酸化型グルタチオンを含んでなり、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に酸化型グルタチオンを施用するための、組成物。 Further, according to one embodiment, the present invention includes the following [1] to [20]. [1] A method for producing leafy vegetables, which comprises a step of applying oxidized glutathione to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination.
[2] The method according to [1], wherein the underground part of the leafy vegetables comprises roots.
[3] The method according to [1] or [2], wherein the total application amount of the oxidized glutathione within the seedling raising period is 0.375 to 1.5 mg per strain.
[4] The method according to any one of [1] to [3], wherein the application period of oxidized glutathione within the seedling raising period is 1 to 5 days.
[5] The method according to any one of [1] to [4], wherein the application period is a period from the day of germination to the seventh day or later.
[6] The method according to [4] or [5], wherein oxidized glutathione is applied daily during the application period.
[7] The method according to any one of [4] to [6], wherein the daily application amount of the oxidized glutathione within the seedling raising period is 0.125 to 0.5 mg per strain.
[8] The method according to any one of [1] to [7], wherein the application of the oxidized glutathione comprises immersing the underground part of the leafy vegetable in an oxidized glutathione-containing medium.
[9] The method according to [8], wherein the oxidized glutathione-containing medium is a nutrient solution.
[10] The method according to any one of [1] to [9], wherein oxidized glutathione is further applied within the growth period on and after the 12th day from the day of germination.
[11] The method according to [10], wherein the total application amount of the oxidized glutathione during the growth period is 0.263 to 2.63 mg per strain.
[12] The application period of oxidized glutathione within the growing period reaches 28 to 40 g per plant (the total weight of the above-ground portion and the below-ground portion) of leafy vegetables from the 12th day from the day of germination. The method according to [10] or [11], which is up to date.
[13] The method according to any one of [10] to [12], wherein during the growth period, the application of oxidized glutathione is carried out for one day or divided into a plurality of days.
[14] The application of oxidized glutathione was carried out on the 12th day from the day of germination, on the day when the weight of leafy vegetables (the sum of the weights of the above-ground portion and the below-ground portion) reached 2.9 to 5.3 g per strain, And the method according to any one of [10] to [13], which is carried out on the day when the weight of leafy vegetables (the sum of the weights of the above-ground portion and the underground portion) reaches 28 to 40 g per strain.
[15] The method according to any one of [10] to [14], wherein the daily application amount of the oxidized glutathione during the growth period is 0.046 to 1.5 mg per strain.
[16] The method according to any one of [1] to [15], which is performed in a closed environment.
[17] The method according to any one of [1] to [16], which is performed in a plant factory.
[18] The leafy vegetables are leaf lettuce, heading lettuce, leafy vegetables in the family Cruciferae, mustard greens (wasabi greens), Mizuna, leaf radish, cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage, The method according to any one of [1] to [17], which is selected from the group consisting of chard (Swiss chard), spinach and chrysanthemum.
[19] Increasing the yield of leafy vegetables, which comprises a step of applying oxidized glutathione to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination Method.
[20] A composition for increasing the yield of leafy vegetables, the composition comprising oxidized glutathione, the composition of leafy vegetables at least within the seedling raising period from the day of germination to the 12th day after germination. A composition for applying oxidized glutathione to the underground.
　また、本発明の別の態様によれば、葉物野菜の収穫量を増加させるための、酸化型グルタチオンの使用であって、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に酸化型グルタチオンを施用することを含んでなる、使用が提供される。 Further, according to another aspect of the present invention, the use of oxidized glutathione for increasing the yield of leafy vegetables, wherein the seedling raising period is at least from the day of germination to the 12th day after germination. In, there is provided a use comprising applying oxidized glutathione to the basement of leafy vegetables.
　また、本発明の別の態様によれば、葉物野菜の収穫量を増加させるための組成物の製造における酸化型グルタチオンの使用であって、上記組成物が酸化型グルタチオンを含み、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に上記組成物が施用されることを含んでなる、使用が提供される。 According to another aspect of the present invention, the use of oxidized glutathione in the manufacture of a composition for increasing the yield of leafy vegetables, wherein the composition contains oxidized glutathione and is at least germinated. Use is provided comprising applying the composition to the basement of leafy vegetables within the seedling raising period from day to 12 days post germination.
　また、本発明の別の態様によれば、葉物野菜の収穫量を増加させるための酸化型グルタチオンであって、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に酸化型グルタチオンを施用することを含んでなる、酸化型グルタチオンが提供される。 Further, according to another aspect of the present invention, the oxidized glutathione for increasing the yield of leafy vegetables, which is a leafy material at least within the seedling raising period from the day of germination to the 12th day after germination. Oxidized glutathione is provided which comprises applying oxidized glutathione to the subterranean portion of a vegetable.
　上記の使用、化合物（酸化型グルタチオン）の態様は何れも、本発明の方法または組成物に関する記載に準じて実施することができる。 Any of the above-mentioned uses and aspects of the compound (oxidized glutathione) can be carried out according to the description regarding the method or composition of the present invention.
　本発明について、実施例を用いて詳しく説明するが、本発明はこれら実施例に限定されるものではない。 The present invention will be described in detail using examples, but the present invention is not limited to these examples.
１.フリルレタスの水耕栽培方法
　完全閉鎖系の環境下において、フリルレタス（リーフレタス(Lactuca sativa var. crispa)の一種）の種子を含水環境下に播種し、レタスの水耕栽培一般に用いられる発芽条件下でインキュベーションした。発芽後、フリルレタスの苗の下部に容器を配置して当該容器に養液を充たし、当該養液に当該フリルレタスの苗の根部が浸漬するように保持し、完全閉鎖系の環境下において水耕栽培を継続した。ここで、前記養液は、市販の水耕栽培用肥料（窒素、リン酸、カリ等を含む)を水で溶解して調製した。照射する人工光源としては、４０００ＫのＬＥＤを使用した。フリルレタスの水耕栽培期間中の１日を、人工光源をフリルレタスに照射する明期と、照射しない暗期に分け、その時間配分は、明期１６時間、暗期８時間とした。温度は１８～２５℃、湿度は５０～９０％とした。なお、農薬は一切使用しなかった。 1. Hydroponic cultivation of frill lettuce In a completely closed environment, seeds of frill lettuce (a type of leaf lettuce (Lactuca sativa var. Crispa)) are sown in a water-containing environment, and generally used for hydroponic cultivation of lettuce. Incubated under germination conditions. After germination, place a container in the lower part of the frill lettuce seedling and fill the container with a nutrient solution, hold the root part of the frill lettuce seedling soaked in the nutrient solution, and water in a completely closed system environment. Cultivation was continued. Here, the nutrient solution was prepared by dissolving a commercially available fertilizer for hydroponics (including nitrogen, phosphoric acid, potassium, etc.) in water. A 4000K LED was used as an artificial light source for irradiation. One day during hydroponics of frill lettuce was divided into a light period in which frill lettuce was irradiated with an artificial light source and a dark period in which it was not irradiated, and the time distribution was 16 hours for the light period and 8 hours for the dark period. The temperature was 18 to 25 ° C. and the humidity was 50 to 90%. No pesticides were used.
２．試験例１
　フリルレタスに、発芽した日から９～１１日目の３日間酸化型グルタチオンを施用した場合の収穫量を確認した。 2. Test example 1
The amount of harvest when the oxidized glutathione was applied to the frill lettuce for 3 days from the day of germination to the day 9 to 11 was confirmed.
試験区１－１および対照区１－１
　試験区１－１では、上述の「１.フリルレタスの水耕栽培方法」に記載された方法によりフリルレタスの水耕栽培を行い、フリルレタスが発芽した日から９～１１日目の合計３日間の間に、１日１回、養液１Ｌ当たり１．５ｍｌのグルタチオン水溶液（市販の酸化型グルタチオン試薬を使用した。酸化型グルタチオン濃度は６０００ｐｐｍ；以下同様である。）を添加した新しい養液と交換した。この１日１回当たりの添加量は、フリルレタス１株当たり０．１８７５ｍｇ（以下、「０．１８７５ｍｇ／株」のように表記する）の酸化型グルタチオン量に相当する。したがって、試験区１－１では、合計で養液１Ｌ当たり４．５ｍｌのグルタチオン水溶液を養液に添加した。この合計量は、０．５６２５ｍｇ／株の酸化型グルタチオン量に相当する。 Test plot 1-1 and control plot 1-1
In test section 1-1, hydroponic cultivation of frill lettuce was carried out by the method described in "1. Hydroponic cultivation of frill lettuce" above, and a total of 3 days from 9 to 11 days from the day on which the frill lettuce germinated. A new nutrient solution to which 1.5 ml of an aqueous glutathione solution (commercially available oxidized glutathione reagent was used. The concentration of oxidized glutathione was 6000 ppm; the same applies hereinafter) was added once a day for 1 day during the day. I replaced it with. The amount added once a day corresponds to the amount of oxidized glutathione of 0.1875 mg (hereinafter referred to as "0.1875 mg / strain") per furyl lettuce strain. Therefore, in test section 1-1, a total of 4.5 ml of an aqueous glutathione solution was added to the nutrient solution per 1 L of the nutrient solution. This total amount corresponds to the amount of oxidized glutathione of 0.5625 mg / strain.
　対照区１－１では、グルタチオン水溶液の添加を行わなかったこと以外は試験区１－１と同条件により、フリルレタスの水耕栽培を同時に行った。 In Control Group 1-1, hydroponic cultivation of frill lettuce was performed simultaneously under the same conditions as in Test Group 1-1 except that the glutathione aqueous solution was not added.
　試験区１－１および対照区１－１で水耕栽培されたフリルレタスを、それぞれ無作為に１３株収穫し、収穫されたフリルレタスの地上部の重量を測定し、その平均値を算出した。さらに、対照区１－１で得られたフリルレタスの地上部の重量の平均値を１００とした場合の試験区１－１で得られたフリルレタスの地上部の重量の平均値の相対値を算出した。結果を表１に示す。
Figure JPOXMLDOC01-appb-T000001
 Frill lettuce hydroponically cultivated in the test plot 1-1 and the control plot 1-1 were randomly harvested in 13 strains respectively, and the weight of the above-ground portion of the harvested furil lettuce was measured, and the average value was calculated. .. Furthermore, the relative value of the average value of the above-ground weight of furyl lettuce obtained in Test Group 1-1 when the average value of the above-ground weight of furyl lettuce obtained in Control Group 1-1 was set to 100 Calculated. The results are shown in Table 1.
Figure JPOXMLDOC01-appb-T000001
　なお、試験区１－１と対照区１－１とを比較すると、１株あたり１８．１ｇの収穫重量が増加したが、これは植物工場において１万株のフリルレタスを生産する場合は、１８１ｋｇの増収となる。 It should be noted that, when the test plot 1-1 and the control plot 1-1 were compared, the harvest weight per plant increased by 18.1 g, which is 181 kg when the plant factory produces 10,000 frill lettuce. Will be increased.
試験区１－２および対照区１－２
　試験区１－２では、フリルレタスが発芽した日から９～１１日目の合計３日間の間に、１日１回、養液１Ｌ当たり３．０ｍｌのグルタチオン水溶液を添加した新しい養液と交換した以外は試験区１－１と同様の方法でフリルレタスの水耕栽培を行った。この１日１回当たりの添加量は、０．３７５ｍｇ／株の酸化型グルタチオン量に相当する。したがって、試験区１－２では、合計で養液１Ｌ当たり９．０ｍｌのグルタチオン水溶液を養液に添加した。この合計量は、１．１２５ｍｇ／株の酸化型グルタチオン量に相当する。 Test plot 1-2 and control plot 1-2
In test area 1-2, during a total of 3 days on the 9th to 11th days after the germination of furyl lettuce, once a day, exchange with a new nutrient solution to which 3.0 ml of glutathione aqueous solution was added per 1 L of nutrient solution. Frill lettuce was hydroponically cultivated in the same manner as in Test Area 1-1 except for the above. The amount added once a day corresponds to the amount of oxidized glutathione of 0.375 mg / strain. Therefore, in test section 1-2, a total of 9.0 ml of an aqueous glutathione solution was added to the nutrient solution per 1 L of the nutrient solution. This total amount corresponds to 1.125 mg / strain of oxidized glutathione.
　対照区１－２では、グルタチオン水溶液の添加を行わなかったこと以外は試験区１－２と同条件により、フリルレタスの水耕栽培を同時に行った。 In Control Group 1-2, hydroponic cultivation of frill lettuce was performed at the same time under the same conditions as in Test Group 1-2 except that the glutathione aqueous solution was not added.
　試験区１－２および対照区１－２で水耕栽培されたフリルレタスの地上部の重量測定、重量の相対値の算出は、試験区１－１と同様の手法により実施した。その結果、試験区１－１と同様の重量増加が認められた。 The weight measurement of the above-ground part of the frill lettuce hydroponically cultivated in the test plot 1-2 and the control plot 1-2 and the calculation of the relative value of the weight were carried out in the same manner as in the test plot 1-1. As a result, the same weight increase as in Test Group 1-1 was observed.
　上述した通り、試験区１－１および試験区１－２では、フリルレタスが発芽した日から９～１１日目の３日間に、養液１Ｌ当たり１．５～３．０ｍｌのグルタチオン水溶液を３回添加した結果、フリルレタスの収穫重量が増加した。 As described above, in the test plots 1-1 and 1-2, 1.5 to 3.0 ml of the glutathione aqueous solution was added to 1 L of the nutrient solution for 3 days on the 9th to 11th days from the day when the frill lettuce germinated. As a result of repeated additions, the harvest weight of frill lettuce increased.
　なお、試験区１－１と同様の条件でフリルレタスを水耕栽培して収穫されたフリルレタスの味覚および風味に関する官能試験を行ったところ、試験区と対照区で特に大きな差は生じなかった。ただし、いくつかの栽培例で、対照区と比較して試験区の方に甘味を感じることがあった。 In addition, when a sensory test on the taste and flavor of furil lettuce harvested by hydroponically cultivating furil lettuce under the same conditions as in test plot 1-1, no significant difference was found between the test plot and control plot. .. However, in some cultivation cases, the test plots sometimes felt sweeter than the control plots.
３．試験例２
　フリルレタスに、発芽した日から１２日目以降酸化型グルタチオンを施用した場合の収穫量を確認した。 3. Test example 2
The amount of harvest when the oxidized glutathione was applied to the frill lettuce on and after the 12th day after germination was confirmed.
試験区２－１および対照区２－１
　試験区２－１では、上述の「１.フリルレタスの水耕栽培方法」に記載された方法によりフリルレタスの水耕栽培を行い、フリルレタスが発芽した日から１２日目（ポスト１回目添加）（発芽した日から１２日目以降の添加を「ポスト…添加」と称する。本明細書において同じ。）、フリルレタスの一株当りの重量（フリルレタスの地上部および地下部の重量の総和とする。）が２．９～５．３ｇに到達した日（ポスト２回目添加）、およびフリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２８～４０ｇに到達した日（ポスト３回目添加）に、それぞれの１回の添加において、養液１Ｌ当たり０．５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。該ポスト１～３回目の添加量は、それぞれ、０．２３ｍｇ／株（ポスト１回目添加）、０．３３ｍｇ／株（ポスト２回目添加）、０．７５ｍｇ／株（ポスト３回目添加）の酸化型グルタチオン量に相当する。したがって、試験区２－１では、合計で１．５ｍｌ／Ｌのグルタチオン水溶液を養液に添加した。この合計量は、１．３１ｍｇ／株の酸化型グルタチオンに相当する。 Test plot 2-1 and control plot 2-1
In test section 2-1, hydroponic cultivation of furil lettuce was carried out by the method described in "1. Hydroponic cultivation of furil lettuce" above, and 12 days after the germination of furil lettuce (post first addition) ) (Addition on and after the 12th day from germination is referred to as “post ... addition”; the same in the present specification), weight per frill lettuce strain (total sum of weight of above-ground portion and below-ground portion of furyl-lettuce) The amount per day (post 2nd addition), and the weight per frill lettuce strain (total sum of above-ground and below-ground parts) reached 28-40 g. On the day (post-third addition), in each single addition, the nutrient solution was replaced with a new one containing 0.5 ml of an aqueous glutathione solution. The amount of addition of the first to third additions was 0.23 mg / strain (first addition of post), 0.33 mg / strain (second addition of post), 0.75 mg / strain (addition of third post), respectively. Equivalent to the amount of type glutathione. Therefore, in test section 2-1, a total of 1.5 ml / L aqueous glutathione solution was added to the nutrient solution. This total amount corresponds to 1.31 mg / strain of oxidized glutathione.
　対照区２－１では、グルタチオン水溶液の添加を行わなかったこと以外は試験区２－１と同条件により、フリルレタスの水耕栽培を試験区２－１と同時に行った。 In the control plot 2-1, hydroponic cultivation of frill lettuce was performed at the same time as the test plot 2-1, under the same conditions as in the test plot 2-1, except that the aqueous glutathione solution was not added.
　試験区２－１および対照区２－１で水耕栽培されたフリルレタスの地上部の重量測定、重量の相対値の算出は、試験例１と同様にして行った。結果を表２に示す。 The weight measurement and calculation of the relative value of the weight of the above-ground part of the frill lettuce hydroponically cultivated in the test plot 2-1 and the control plot 2-1 were performed in the same manner as in Test Example 1. The results are shown in Table 2.
試験区２－２および対照区２－２
　試験区２－２では、上述の「１.フリルレタスの水耕栽培方法」に記載された方法によりフリルレタスの水耕栽培を行い、フリルレタスが発芽した日から１２日目（ポスト１回目添加）およびフリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２．９～５．３ｇに到達した日（ポスト２回目添加）に、それぞれの１回の添加において、養液１Ｌ当たり０．５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。さらにフリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２８～４０ｇに到達した日（ポスト３回目添加）に、養液１Ｌ当たり１５ｍｌのグルタチオン水溶液を添加した。該ポスト１～３回目の添加量は、それぞれ、０．２３ｍｇ／株（ポスト１回目添加）、０．３３ｍｇ／株（ポスト２回目添加）、２２．５ｍｇ／株（ポスト３回目添加）の酸化型グルタチオン量に相当する。したがって、試験区２－２では、合計で１６ｍｌ／Ｌのグルタチオン水溶液を養液に添加した。この合計量は、２３．１ｍｇ／株の酸化型グルタチオン量に相当する。 Test area 2-2 and control area 2-2
In test section 2-2, hydroponic cultivation of frill lettuce was performed by the method described in "1. Hydroponic cultivation of frill lettuce" above, and 12 days after the day when the frill lettuce germinated (post first addition) ) And frill lettuce per strain (sum of the weight of the above-ground part and the underground part) reached 2.9 to 5.3 g (post second addition), in each one addition, It was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the solution. Further, on the day when the weight of the furyl lettuce per strain (sum of the weights of the above-ground portion and the underground portion) reached 28 to 40 g (post third addition), 15 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution. The amount of addition of the first to third post was 0.23 mg / strain (first post addition), 0.33 mg / strain (second post addition), 22.5 mg / strain (post third addition), respectively. Equivalent to the amount of type glutathione. Therefore, in test section 2-2, a total of 16 ml / L of glutathione aqueous solution was added to the nutrient solution. This total amount corresponds to the amount of oxidized glutathione of 23.1 mg / strain.
　対照区２－２では、グルタチオン水溶液の添加を行わなかったこと以外は試験区２－２と同条件により、フリルレタスの水耕栽培を試験区２－２と同時に行った。 In the control plot 2-2, hydroponic cultivation of frill lettuce was performed at the same time as the test plot 2-2 under the same conditions as in the test plot 2-2 except that the glutathione aqueous solution was not added.
　試験区２－２および対照区２－２で水耕栽培されたフリルレタスの地上部の重量測定、重量の相対値の算出は、試験区１－１と同様の手法により実施した。結果を表２に示す。　 The weight measurement of the above-ground part of the frill lettuce hydroponically cultivated in the test plot 2-2 and the control plot 2-2 and the calculation of the relative value of the weight were carried out in the same manner as in the test plot 1-1. The results are shown in Table 2. ‥
試験区２－３
　試験区２－３では、上述の「１.フリルレタスの水耕栽培方法」に記載された方法によりフリルレタスの水耕栽培を行い、フリルレタスが発芽した日から１２日目（ポスト１回目添加）に、養液１Ｌ当たり０．５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。また、フリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２．９～５．３ｇに到達した日（ポスト２回目添加）に、養液１Ｌ当たり１ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。さらに、フリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２８～４０ｇに到達した日（ポスト３回目添加）に、養液１Ｌ当たり５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。該ポスト１～３回目の添加量は、それぞれ、０．２３ｍｇ／株（ポスト１回目添加）、０．６７ｍｇ／株（ポスト２回目添加）、７．５ｍｇ／株（ポスト３回目添加）の酸化型グルタチオン量に相当する。したがって、試験区２－３では、合計で６．５ｍｌ／Ｌのグルタチオン水溶液を養液に添加した。この合計量は、８．４ｍｇ／株の酸化型グルタチオン量に相当する。 Test area 2-3
In test section 2-3, hydroponic cultivation of frill lettuce was carried out by the method described in "1. Hydroponic cultivation of frill lettuce" above, and 12 days from the day on which the frill lettuce germinated (post first addition) ) Was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution. In addition, on the day when the weight (total sum of the weight of the above-ground part and the amount of the below-ground part) of the frill lettuce reached 2.9 to 5.3 g (post second addition), 1 ml of glutathione aqueous solution was added per 1 L of the nutrient solution. The new nutrient solution added was replaced. Furthermore, on the day when the weight (total sum of weight above and below ground) per furyl lettuce reached 28 to 40 g (post third addition), 5 ml of glutathione aqueous solution was added per 1 L of nutrient solution. Replaced with liquid. The post-first to third addition amounts were 0.23 mg / strain (post first addition), 0.67 mg / strain (post second addition), and 7.5 mg / strain (post third addition) oxidation, respectively. Equivalent to the amount of type glutathione. Therefore, in test section 2-3, a total of 6.5 ml / L glutathione aqueous solution was added to the nutrient solution. This total amount corresponds to the amount of oxidized glutathione of 8.4 mg / strain.
　対照区２－３では、グルタチオン水溶液の添加を行わなかったこと以外は試験区２－３と同条件により、フリルレタスの水耕栽培を試験区２－２と同時に行った。 In control plot 2-3, hydroponic cultivation of frill lettuce was carried out at the same time as test plot 2-2 under the same conditions as in test plot 2-3 except that the glutathione aqueous solution was not added.
　試験区２－３および対照区２－３で水耕栽培されたフリルレタスの地上部の重量測定、重量の相対値の算出は、試験区１－１と同様の手法により実施した。結果を表２に示す。

Figure JPOXMLDOC01-appb-T000002
 The weight measurement and calculation of the relative value of the weight of the above-ground part of the frill lettuce hydroponically cultivated in the test plot 2-3 and the control plot 2-3 were carried out by the same method as in the test plot 1-1. The results are shown in Table 2.

Figure JPOXMLDOC01-appb-T000002
　表２によれば、フリルレタスが発芽した日から１２日目日以降の生育期間内のみにグルタチオン水溶液を添加した場合、フリルレタスの増収効果は認められなかった。 According to Table 2, when the glutathione aqueous solution was added only during the growth period from the day 12 of the germination of the frill lettuce to the day after the germination, the effect of increasing the yield of the frill lettuce was not observed.
４．試験例３
　フリルレタスに、発芽した日から９～１１日目の３日間に酸化型グルタチオンを施用し、さらに発芽した日から１２日目およびそれ以降にグルタチオン水溶液を複数回に分けて施用した場合の収穫量を確認した。 4. Test example 3
Yield when oxidized glutathione was applied to frill lettuce for 3 to 9 to 11 days after germination, and glutathione aqueous solution was applied in multiple times from 12 to 12 days after germination. It was confirmed.
試験区３－１
　試験区３－１では、上述の「１.フリルレタスの水耕栽培方法」に記載された方法によりフリルレタスの水耕栽培を行い、フリルレタスが発芽した日から９～１１日目の合計３日間の間に、１日１回、養液１Ｌ当たり３．０ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。この１日１回当たりの添加量は、０．３７５ｍｇ／株の酸化型グルタチオン量に相当する。さらに、試験区３－１では、フリルレタスが発芽した日から１２日目(ポスト１回目添加)、フリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２．９～５．３ｇに到達した日(ポスト２回目添加)、およびフリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２８～４０ｇに到達した日(ポスト３回目添加)に、それぞれの１回の添加において、養液１Ｌ当たり０．５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。該ポスト１～３回目の添加量は、０．２３ｍｇ／株（ポスト１回目）、０．３３ｍｇ／株（ポスト２回目）、０．７５ｍｇ／株（ポスト３回目）の酸化型グルタチオン量に相当する。 Test area 3-1
In test section 3-1, hydroponic cultivation of frill lettuce was carried out by the method described in “1. Hydroponic cultivation of frill lettuce” above, and a total of 3 days from the day when the frill lettuce germinated to the 11th to 11th days. During the day, the fresh nutrient solution was added once a day with 3.0 ml of an aqueous glutathione solution added per liter of the nutrient solution. The amount added once a day corresponds to the amount of oxidized glutathione of 0.375 mg / strain. Furthermore, in the test plot 3-1, the weight of the furyl lettuce per strain (the total weight of the above-ground portion and the underground portion) was 2.9 to 12 days after the germination of the furyl lettuce (the first post addition). On the day of reaching 5.3 g (post second addition), and the day when the weight of the furyl lettuce per strain (sum of the weight of the above-ground part and the underground part) reached 28-40 g (post third addition), In each single addition, a new nutrient solution containing 0.5 ml of an aqueous glutathione solution per 1 L of nutrient solution was replaced. The amount added at the 1st to 3rd post corresponds to the amount of oxidized glutathione at 0.23 mg / strain (first post), 0.33 mg / strain (second post), 0.75 mg / strain (third post). To do.
　対照区３－１では、グルタチオン水溶液の添加を行わなかったこと以外は試験区３－１と同条件により、フリルレタスの水耕栽培を試験区３－１と同時に行った。 In the control section 3-1, hydroponic cultivation of frill lettuce was carried out at the same time as the test section 3-1 under the same conditions as in the test section 3-1, except that the aqueous glutathione solution was not added.
　試験区３－１および対照区３－１で水耕栽培されたフリルレタスの地上部の重量測定、重量の相対値の算出は、試験例１と同様にして行った。結果を表３に示す。 The weight measurement of the above-ground portion of furyl lettuce hydroponically cultivated in the test section 3-1 and the control section 3-1 and the calculation of the relative value of the weight were performed in the same manner as in Test Example 1. The results are shown in Table 3.
試験区３－２および対照区３－２
　試験区３－２では、上述の「１.フリルレタスの水耕栽培方法」に記載された方法によりフリルレタスの水耕栽培を行い、フリルレタスが発芽した日から９～１１日目の合計３日間の間に、１日１回、養液１Ｌ当たり１．５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。この１日１回当たりの添加量は、０．１８７５ｍｇ／株の酸化型グルタチオン量に相当する。さらに、試験区３－２では、フリルレタスが発芽した日から１２日目（ポスト１回目添加）、フリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２．９～５．３ｇに到達した日（ポスト２回目添加）およびフリルレタスの一株当りの重量（地上部および地下部の重量の総和）が２８～４０ｇに到達した日（ポスト３回目添加）に、それぞれ１回の添加において、養液１Ｌ当たり０．５ｍｌのグルタチオン水溶液を添加した新しい養液と交換した。該ポスト１～３回目の添加量はそれぞれ、０．２３ｍｇ／株（ポスト１回目添加）、０．３３ｍｇ／株（ポスト２回目添加）、０．７５ｍｇ／株（ポスト３回目添加）の酸化型グルタチオン量に相当する。 Test plot 3-2 and control plot 3-2
In test area 3-2, hydroponic cultivation of furil lettuce was carried out by the method described in “1. Hydroponic cultivation method of furil lettuce”, and a total of 3 days from the day when germinated furil lettuce was germinated. During the day, the fresh nutrient solution was added once a day with the addition of 1.5 ml of glutathione aqueous solution per liter of nutrient solution. The amount added once a day corresponds to the amount of oxidized glutathione of 0.1875 mg / strain. Furthermore, in test section 3-2, the weight per 1 day of the furyl lettuce (total of the weight of the above-ground portion and the amount of the below-ground portion) was 2.9 to 12 days after the germination of the furyl lettuce (first addition of post). On the day of reaching 5.3 g (post second addition) and on the day when the weight of the furyl lettuce per strain (sum of aboveground and underground parts) reached 28-40 g (post third addition), respectively. In one addition, it was replaced with a new nutrient solution to which 0.5 ml of an aqueous glutathione solution was added per 1 L of the nutrient solution. The post-first to third addition amounts were 0.23 mg / strain (post first addition), 0.33 mg / strain (post second addition), and 0.75 mg / strain (post third addition) oxidized form, respectively. Equivalent to the amount of glutathione.
　対照区３－２では、グルタチオン水溶液の添加を行わなかったこと以外は試験区３－２と同条件により、フリルレタスの水耕栽培を試験区３－２と同時に行った。 In control section 3-2, hydroponic cultivation of frill lettuce was carried out at the same time as test section 3-2 under the same conditions as in test section 3-2 except that the glutathione aqueous solution was not added.
　試験区３－２および対照区３－２で水耕栽培されたフリルレタスの地上部の重量測定、重量の相対値の算出は、試験例１と同様にして行った。結果を表３に示す。
Figure JPOXMLDOC01-appb-T000003
 The weight measurement and calculation of the relative weight of the above-ground parts of the furil lettuce hydroponically cultivated in the test plot 3-2 and the control plot 3-2 were performed in the same manner as in Test Example 1. The results are shown in Table 3.
Figure JPOXMLDOC01-appb-T000003
　表３によれば、試験区３－１および試験区３－２では、フリルレタスが発芽した日から９～１１日目の３日間とフリルレタスが発芽した日から１２日目以降にそれぞれグルタチオン水溶液を複数回に分けて添加した結果、収穫されるフリルレタスの重量は増加した。　 According to Table 3, in the test plots 3-1 and 3-2, the glutathione aqueous solution was obtained on the 9th to 11th days after the germination of the frill lettuce and on the 12th day after the germination of the frill lettuce. As a result of the addition of a plurality of times, the weight of the harvested frill lettuce was increased. ‥
　さらに、フリルレタスが発芽した日から９～１１日目の３日間のグルタチオンの添加量を１日１回、養液１Ｌ当たり５．０ｍｌにする以外、試験区３－１と同様の方法により試験を行った。その結果、フリルレタスの増収効果は認められなかった Furthermore, the test was conducted in the same manner as in Test Group 3-1 except that the addition amount of glutathione was changed to 5.0 ml per 1 L of the nutrient solution once a day for 3 to 9 to 11 days from the day on which the frill lettuce germinated. I went. As a result, no sales-increasing effect of frill lettuce was observed.
　なお、試験区３－１と対照区３－１とを比較すると、１株あたり１１ｇ収穫重量が増加したが、これは植物工場において１万株のフリルレタスを生産する場合は、１１０ｋｇの増収となる。 In addition, when the test plot 3-1 and the control plot 3-1 were compared, the harvest weight per plant increased by 11 g, which is 110 kg higher when the plant factory produces 10,000 floryl lettuce plants. Become.

Claims (20)

  1. 　少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜を生産する方法。 A method for producing leafy vegetables, which comprises a step of applying oxidized glutathione to the underground part of the leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination.
  2. 　前記葉物野菜の地下部が根を含んでなる、請求項１に記載の方法。 The method according to claim 1, wherein the underground part of the leafy vegetables comprises roots.
  3. 　前記育苗期間内における前記酸化型グルタチオンの総施用量が、１株当たり０．３７５～１．５ｍｇである、請求項１または２に記載の方法。 The method according to claim 1 or 2, wherein the total application amount of the oxidized glutathione within the seedling raising period is 0.375 to 1.5 mg per strain.
  4. 　前記育苗期間内における酸化型グルタチオンの施用期間が、１～５日である、請求項１～３のいずれか一項に記載の方法。 The method according to any one of claims 1 to 3, wherein the application period of oxidized glutathione within the seedling raising period is 1 to 5 days.
  5. 　前記施用期間が、発芽した日から７日目以降の期間である、請求項１～４のいずれか一項に記載の方法。 The method according to any one of claims 1 to 4, wherein the application period is a period from the 7th day after germination to the 7th day or later.
  6. 　前記施用期間において酸化型グルタチオンを毎日施用する、請求項４または５に記載の方法。 The method according to claim 4 or 5, wherein oxidized glutathione is applied daily during the application period.
  7. 　前記育苗期間内における前記酸化型グルタチオンの１日当たりの施用量が、１株当たり０．１２５～０．５ｍｇである、請求項４～６のいずれか一項に記載の方法。 The method according to any one of claims 4 to 6, wherein the daily application amount of the oxidized glutathione in the seedling raising period is 0.125 to 0.5 mg per strain.
  8. 　前記酸化型グルタチオンの施用が、酸化型グルタチオン含有培地中に葉物野菜の地下部を浸漬することを含んでなる、請求項１～７のいずれか一項に記載の方法。 The method according to any one of claims 1 to 7, wherein the application of the oxidized glutathione comprises immersing the underground part of the leafy vegetable in a medium containing oxidized glutathione.
  9. 　前記酸化型グルタチオン含有培地が養液である、請求項８に記載の方法。 The method according to claim 8, wherein the medium containing oxidized glutathione is a nutrient solution.
  10. 　発芽した日から１２日目以降の生育期間内にさらに酸化型グルタチオンを施用する、請求項１～９のいずれか一項に記載の方法。 10. The method according to any one of claims 1 to 9, wherein oxidized glutathione is further applied within the growth period on and after the 12th day from the day of germination.
  11. 　前記生育期間内における前記酸化型グルタチオンの総施用量が、１株当たり０．２６３～２．６３ｍｇである、請求項１０に記載の方法。 11. The method according to claim 10, wherein the total application amount of the oxidized glutathione during the growth period is 0.263 to 2.63 mg per strain.
  12. 　前記生育期間内における酸化型グルタチオンの施用期間が、発芽した日から１２日目から葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日までである、請求項１０または１１に記載の方法。 The period of application of oxidized glutathione within the growth period is from the day 12 from the day of germination until the day when the weight of leafy vegetables (the sum of the weights of the above-ground part and the below-ground part) reaches 28 to 40 g per strain. The method according to claim 10 or 11, wherein:
  13. 　前記生育期間内において、酸化型グルタチオンの施用を１日でまたは複数日に分けて実施する、請求項１０～１２のいずれか一項に記載の方法。 The method according to any one of claims 10 to 12, wherein the application of oxidized glutathione is carried out in one day or divided into a plurality of days within the growing period.
  14. 　酸化型グルタチオンの施用を、発芽した日から１２日目、葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２．９～５．３ｇに到達する日、および葉物野菜の重量（地上部および地下部の重量の総和）が１株当たり２８～４０ｇに到達する日に実施する、請求項１０～１３のいずれか一項に記載の方法。 On the 12th day from the day of germination, the application of oxidized glutathione, the day when the weight of leafy vegetables (the sum of the weights of the above-ground part and the below-ground part) reached 2.9 to 5.3 g per strain, and the leaves. The method according to any one of claims 10 to 13, which is carried out on the day when the weight of vegetables (the sum of the weight of the above-ground portion and the weight of the below-ground portion) reaches 28 to 40 g per share.
  15. 　前記生育期間内における前記酸化型グルタチオンの１日当たりの施用量が、１株当たり０．０４６～１．５ｍｇである、請求項１０～１４のいずれか一項に記載の方法。 The method according to any one of claims 10 to 14, wherein the daily application amount of the oxidized glutathione during the growth period is 0.046 to 1.5 mg per strain.
  16. 　閉鎖環境下で行われる、請求項１～１５のいずれか一項に記載の方法。 The method according to any one of claims 1 to 15, which is performed in a closed environment.
  17. 　植物工場で行われる、請求項１～１６のいずれか一項に記載の方法。 The method according to any one of claims 1 to 16, which is performed in a plant factory.
  18. 　前記葉物野菜が、リーフレタス、結球レタス、アブラナ科の葉菜、からし菜（わさび菜）、ミズナ、葉ダイコン、白菜非結球品種、白菜結球性ミニ品種、非結球性キャベツ、フダンソウ（スイスチャード）、ホウレンソウおよびシュンギクからなる群から選択される、請求項１～１７のいずれか一項に記載の方法。 The leafy vegetables include leaf lettuce, heading lettuce, cruciferous leafy vegetables, mustard vegetables (wasabi greens), Mizuna, leaf radish, Chinese cabbage non-heading variety, Chinese cabbage heading mini variety, non-heading cabbage, chard root (Switzerland) The method according to any one of claims 1 to 17, wherein the method is selected from the group consisting of chard), spinach and chrysanthemum.
  19. 　少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において、葉物野菜の地下部に酸化型グルタチオンを施用する工程を含んでなる、葉物野菜の収穫量を増加させる方法。 A method of increasing the yield of leafy vegetables, which comprises a step of applying oxidized glutathione to the underground part of the leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination.
  20. 　葉物野菜の収穫量を増加させるための組成物であって、酸化型グルタチオンを含んでなり、少なくとも発芽した日から発芽後１２日目に至るまでの育苗期間内において葉物野菜の地下部に酸化型グルタチオンを施用するための、組成物。 A composition for increasing the yield of leafy vegetables, comprising oxidized glutathione, which is added to the underground part of leafy vegetables at least during the seedling raising period from the day of germination to the 12th day after germination. A composition for applying oxidized glutathione.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023171639A1 (en) * 2022-03-07 2023-09-14 株式会社カネカ Agricultural composition and method for cultivating plants using same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008072602A1 (en) * 2006-12-11 2008-06-19 Japan Science And Technology Agency Plant growth regulator and use thereof
WO2013088956A1 (en) * 2011-12-12 2013-06-20 岡山県 Compound for increasing amino acid content in plant, and use thereof
WO2017006869A1 (en) * 2015-07-03 2017-01-12 株式会社カネカ Fertilizer composition for application to leaves and including oxidized glutathione and fertilizer component
JP2017063632A (en) * 2015-09-28 2017-04-06 公立大学法人秋田県立大学 Hydroponic method, leaf vegetables, culture liquid, and culture concentrate composition
JP2018121590A (en) * 2017-02-02 2018-08-09 昭和電工株式会社 Seedling growth method using artificial light

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008072602A1 (en) * 2006-12-11 2008-06-19 Japan Science And Technology Agency Plant growth regulator and use thereof
WO2013088956A1 (en) * 2011-12-12 2013-06-20 岡山県 Compound for increasing amino acid content in plant, and use thereof
WO2017006869A1 (en) * 2015-07-03 2017-01-12 株式会社カネカ Fertilizer composition for application to leaves and including oxidized glutathione and fertilizer component
JP2017063632A (en) * 2015-09-28 2017-04-06 公立大学法人秋田県立大学 Hydroponic method, leaf vegetables, culture liquid, and culture concentrate composition
JP2018121590A (en) * 2017-02-02 2018-08-09 昭和電工株式会社 Seedling growth method using artificial light

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023171639A1 (en) * 2022-03-07 2023-09-14 株式会社カネカ Agricultural composition and method for cultivating plants using same

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