WO2023210047A1 - Accélérateur d'activité nodulaire - Google Patents

Accélérateur d'activité nodulaire Download PDF

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Publication number
WO2023210047A1
WO2023210047A1 PCT/JP2022/041999 JP2022041999W WO2023210047A1 WO 2023210047 A1 WO2023210047 A1 WO 2023210047A1 JP 2022041999 W JP2022041999 W JP 2022041999W WO 2023210047 A1 WO2023210047 A1 WO 2023210047A1
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Prior art keywords
ascorbic acid
soybeans
mass
ppm
soybean
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PCT/JP2022/041999
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English (en)
Japanese (ja)
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春香 前田
厚輝 大西
圭二 遠藤
彰子 川崎
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花王株式会社
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Publication of WO2023210047A1 publication Critical patent/WO2023210047A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

Definitions

  • the present invention relates to a root nodule activity promoter that promotes root nodule activity in soybean.
  • soybean which has a large amount of protein per unit area, is extremely important for the world's food supply as a high-protein and high-calorie crop. Soybeans can be eaten as is, or they can be pressed into soybean oil and high-protein soybean meal. It is also used as a raw material for lecithin, a natural emulsifier.
  • Soybean oil is used as a raw material for daily necessities such as cosmetics and soaps, and is also used as biofuel. Soybean meal is also important as feed for livestock.
  • Rhizobia are soil microorganisms that form nodules on the roots of legumes and perform symbiotic nitrogen fixation. Rhizobacteria reduce nitrogen in the atmosphere in their root nodules, converting it into ammonia nitrogen, and further converting it into ureides such as allantoin and allantoic acid, or amides such as asparagine, and supplies them to the host. Soybean absorbs a large amount of nitrogen, most of which is said to come from rhizobia. Therefore, in order to ensure soybean productivity, it is considered important to strengthen root nodules, and techniques have been reported, such as adding specific compounds to propagate and activate rhizobia.
  • Patent Document 1 states that N-acyllactam compounds have a rhizobial growth effect (Patent Document 1), and that when soybean is inoculated with an inoculant containing glycine betaine, the number of root nodules, nodule weight, It has been reported that the dry weight increases (Patent Document 2).
  • Non-Patent Document 1 discloses that when olives were sprayed twice with 500, 1,000, and 2,000 ppm of ascorbic acid on their leaves, the number of leaves increased.
  • Non-Patent Document 2 discloses that when chickpea, a plant belonging to the leguminous family, is treated with a total of three foliar spray treatments of 100, 200, and 300 ppm ascorbic acid aqueous solution, a yield increase effect of up to 30% can be exhibited.
  • Non-Patent Document 1 JP-A-63-304977
  • Patent Document 2 JP-A-2003-40720
  • Patent Document 3 JP-A-2009-73772
  • Non-Patent Document 1 Mayi et al., (2014) ) Effect of Foliar Spray of Humic acid, Ascorbic acid, Cultivars and their Interactions on Growth of Olive (Olea European L.) Transplants cvs. Khithairy and Sorany. IOSR Journal of Agriculture and Veterinary Science, 7: 18-30.
  • Non-patent Document 2 Zarghamnejad et al., (2014) Chickpea response to ascorbic acid foliar application at vegetable and reproductive stages. International Journal of Biosciences, 5: 166-170.
  • the present invention relates to the following 1) to 6).
  • a root nodule activity promoter for soybeans containing ascorbic acid or a salt thereof as an active ingredient which is applied to soybeans at a concentration of 5,000 to 20,000 mass ppm as ascorbic acid.
  • a soybean growth promoter containing ascorbic acid or a salt thereof as an active ingredient which is applied to soybeans at a concentration of 5,000 to 20,000 mass ppm as ascorbic acid.
  • a method for promoting root nodule activity in soybeans comprising a step of applying a treatment solution containing ascorbic acid or a salt thereof at 5,000 to 20,000 mass ppm to soybeans.
  • a method for promoting the growth of soybeans comprising a step of applying a treatment solution containing ascorbic acid or a salt thereof to soybeans at 5,000 to 20,000 mass ppm.
  • a treatment solution containing ascorbic acid or a salt thereof to soybeans at 5,000 to 20,000 mass ppm.
  • Use of ascorbic acid or a salt thereof for producing a soybean root nodule activity promoter applied to soybeans at a concentration of 5,000 to 20,000 mass ppm as ascorbic acid Use of ascorbic acid or a salt thereof for producing a soybean growth promoter applied to soybeans at a concentration of 5,000 to 20,000 mass ppm as ascorbic acid.
  • Amount of allantoin in soybean exudate one week after foliar spraying of ascorbic acid Amount of allantoic acid in soybean exudate one week after foliar spraying of ascorbic acid. Soybean plants one week after foliar spraying of ascorbic acid. Chickpea root nodule weight one week after foliar spraying of ascorbic acid. Amount of allantoic acid in chickpea exudate one week after foliar spraying of ascorbic acid. Amount of asparagine in chickpea exudate one week after foliar spraying of ascorbic acid. Seed weight per individual of Lotus japonicus one week after foliar spraying of ascorbic acid. Number of pods per individual of Lotus japonicus one week after foliar spraying of ascorbic acid. Seed weight per pod of Lotus japonicus one week after foliar spraying of ascorbic acid.
  • the present invention relates to providing a method and material for promoting the growth of soybeans.
  • the present inventors have discovered that a high concentration of ascorbic acid has the effect of improving the amount of ureide produced in soybeans with attached root nodules, and is useful for improving the grain yield of soybeans.
  • root nodule refers to a nodule that occurs on the root of a plant due to symbiosis with bacteria (rhizobia). Rhizobia perform so-called symbiotic nitrogen fixation, reducing atmospheric nitrogen in the root nodules, converting it into ammonia nitrogen, and supplying it to the host.
  • Rhizobium is said to be specific for plants with which they can coexist, and examples of rhizobia that host soybean include Bradyrhizobium diazoefficiens and Bradyrhizobium diazoefficiens. Known species include Bradyrhizobium japonicum, Bradyrhizobium elkanii, and Ensifer fredii.
  • soybean refers to soybean (scientific name: Glycine max), which is an annual plant of the Fabaceae family.
  • the varieties include Fukuyutaka, Enrei, Sato no Hohoemi, Yuagari Musume, Ryuhou, Suzuyutaka, etc., but the present invention is not limited thereto.
  • ascorbic acid may be any of the D-form, L-form, and DL-form, but it is preferably the L-form (so-called L-ascorbic acid).
  • L-ascorbic acid commercially available products of various grades can be used.
  • the salts of ascorbic acid include salts with nitrogen-containing organic bases such as sodium salts, potassium salts, calcium salts, magnesium salts, barium salts, ammonium salts, pyridine, trimethylamine, triethylamine, tributylamine, and diethylamine. can.
  • the use of ascorbic acid or its salt at a certain concentration or more can act as a root nodule activity promoter or soybean growth promoter, and can be used to promote nodule activity in soybeans or soybean growth. It can be used to produce a root nodule activity promoter or a soybean growth promoter in soybean.
  • nodule activity refers to the nitrogen-fixing function performed by root nodules in the host plant
  • promoting nodule activity refers to promoting the nitrogen-fixing function performed by root nodules.
  • the nitrogen fixation function in the host plant can be evaluated as ureide production ability or amide production ability, and therefore, the nodule activity of the present invention can be more specifically evaluated as the amount of ureide production or amide production per wet root nodule weight.
  • the amount of ureide or amide produced can be calculated by measuring the amount of ureide or amide in the exudate collected when the plant is cut (for example, the above-ground part is cut just below the cotyledons).
  • examples of ureido include allantoin, allantoic acid, or citrulline
  • examples of amides include asparagine or glutamine, and it is preferable to measure the amount of allantoic acid or asparagine.
  • promoting soybean growth means increasing the growth amount (fresh weight, elongation amount, etc.) of soybean and increasing the yield of fruits, seeds, or grains, and preferably, increasing the yield of fruits, seeds, or grains. This is an increase in yield.
  • the above root nodule activity promoter or soybean growth promoter is a composition for promoting root nodule activity in soybean or growth of soybean (e.g., various agricultural or horticultural materials), or added to various agricultural or horticultural materials.
  • ⁇ It can be a material (single substance) or a preparation for compounding.
  • the form of the composition may be a liquid or gel composition, or a solid state (block, powder, granule, etc.) composition. That is, the composition may be in a liquid form that can be applied directly to soybean plants, or it may be a composition that is diluted or dissolved before use.
  • the above composition may contain any other components in addition to the ascorbic acid or its salt of the present invention.
  • Such components include solvents (e.g., water, buffers, media, hydroponic solutions, etc.), carriers (diatomaceous earth, vermiculite, perlite, peat moss, activated carbon, humus, talc, zeolite, clay, carbon black, pulp). , straw, soybean meal, bentonite, kaolin, montmorillonite, alumina, etc.), pH adjuster, spreading agent to increase the ability to spread to plants, fertilizer ingredients to increase fertilizer effectiveness, pesticide ingredients, binders, fillers.
  • solvents e.g., water, buffers, media, hydroponic solutions, etc.
  • carriers diatomaceous earth, vermiculite, perlite, peat moss, activated carbon, humus, talc, zeolite, clay, carbon black, pulp.
  • straw soybean meal, bentonite, kaolin, montmorillonite
  • plant growth promoting microorganisms such as rhizobia and mycorrhizal fungi, essential nutrients for plants, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleobases, sugars, monohydric alcohols, nonionic surfactants, food additives microbial extracts, plant hormones, nod factors or lipo-chitooligosaccharides, synthetic lipo-chitooligosaccharides, chitooligosaccharides, chitinic compounds, linoleic acid or its derivatives, linolenic acid or its derivatives, karrikin, acyl-homoserine lactones.
  • plant growth promoting microorganisms such as rhizobia and mycorrhizal fungi, essential nutrients for plants, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleobases, sugars, monohydr
  • compositions include agricultural or horticultural materials such as fertilizers, water for watering, soil conditioners, pesticides, and plant supplements (e.g., activators, nutrients, etc.); Not limited.
  • the composition may be prepared by adding ascorbic acid or a salt thereof to conventional agricultural or horticultural materials.
  • the content of ascorbic acid or its salt in the root nodule activity promoter or soybean growth promoter of the present invention can be appropriately set to suit the application amount, but for example, in the total mass of the composition, It is 50% by mass or more, preferably 60% by mass or more, more preferably 70% by mass or more, and 100% by mass or less, preferably 98% by mass or less, more preferably 95% by mass or less. Further, it is 50 to 100% by mass, preferably 60 to 98% by mass, and more preferably 70 to 95% by mass.
  • the root nodule activity promoter or soybean growth promoter of the present invention may be applied to soybeans as long as it is supplied to soybeans so as to exhibit the effects of the present invention, but preferably by spraying the composition so that it comes into contact with soybean plants.
  • a spray liquid is prepared as is or by diluting with water, preferably ion-exchanged water, and if the composition is in solid form, it is mixed with water, preferably ion-exchanged water.
  • a spray solution is prepared by dissolving the solution and supplied to soybean plants.
  • the method of spraying is not particularly limited, but examples thereof include a spray method, that is, a method of spraying the spray liquid in a mist form.
  • the spreadability is well expressed on the plant.
  • spray methods include manual spraying using a mister, atomizer, spreader (e.g., boom sprayer), and aerial spraying using an airplane, helicopter, drone, etc. can be mentioned.
  • the application amount of the root nodule activity promoter or soybean growth promoter may vary depending on the application method, application time, cultivation density, growth stage, etc.;
  • the concentration in the spray liquid is 5,000 mass ppm or more, preferably 8,000 mass ppm or more, more preferably 10,000 mass ppm or more, and 20,000 mass ppm or less, preferably 18,000 mass ppm.
  • the mass is more preferably 15,000 ppm or less. Further, it is 5,000 to 20,000 mass ppm, preferably 8,000 to 18,000 mass ppm, and more preferably 10,000 to 15,000 mass ppm.
  • the amount of ascorbic acid applied per soybean plant is 33 mg or more, preferably 53 mg or more, more preferably 66 mg or more, and 133 mg or less, preferably 120 mg or less, more preferably 100 mg or less. Further, it is 33 to 133 mg, preferably 53 to 120 mg, and more preferably 66 to 100 mg.
  • the root nodule activity promoter or soybean growth promoter may be applied in an amount within the above range at once, or may be applied in multiple doses.
  • the timing and number of applications are generally determined by Fehr et al. R4 (pod elongation stage), R5 (early grain enlargement stage), R6 (grain enlargement stage) in the growth stage display of the growth period, that is, around 5 to 13 weeks after sowing, preferably around 6 to 12 weeks after sowing, 1 It is preferred to apply once or 1 to 3 times.
  • the present invention further discloses the following aspects.
  • a root nodule activity promoter in soybeans containing ascorbic acid or a salt thereof as an active ingredient which is applied to soybeans at a concentration of 5,000 to 20,000 mass ppm as ascorbic acid.
  • a soybean growth promoter containing ascorbic acid or a salt thereof as an active ingredient which is applied to soybeans at a concentration of 5,000 to 20,000 mass ppm as ascorbic acid.
  • ⁇ 4> A method for promoting root nodule activity in soybeans, comprising a step of applying a treatment solution containing ascorbic acid or its salt to soybeans at 5,000 to 20,000 mass ppm.
  • ⁇ 5> A method for promoting the growth of soybeans, comprising a step of applying a treatment solution containing ascorbic acid or a salt thereof to soybeans at 5,000 to 20,000 mass ppm.
  • ⁇ 6> The method according to ⁇ 4> or ⁇ 5>, wherein the application to soybeans is foliar spraying.
  • ⁇ 7> The method according to ⁇ 6>, wherein the method is applied to soybean once or once to three times during the grain enlargement period or 6 to 12 weeks after soybean sowing.
  • the application amount as ascorbic acid is preferably 8,000 mass ppm or more, more preferably 10,000 mass ppm or more as a concentration in the spray solution when spraying on the leaves of soybean.
  • the mass ppm or more and preferably 18,000 mass ppm or less, more preferably mass 15,000 ppm or less, or preferably 8,000 to 18,000 mass ppm, more preferably 10,000 to 15,000 mass ppm.
  • the mass is ppm.
  • the amount of ascorbic acid applied per soybean plant is 33 to 133 mg.
  • the content of ascorbic acid or its salt in the composition is 50% by mass or more, preferably 60% by mass or more, more preferably 60% by mass or more based on the total mass of the composition. is 70% by mass or more and 100% by mass or less, preferably 98% by mass or less, more preferably 95% by mass or less, or 50 to 100% by mass, preferably 60 to 98% by mass, more preferably It is 70 to 95% by mass.
  • Example 1 Growth-promoting effect of ascorbic acid on soybean The effect of single foliar spraying of high-concentration ascorbic acid on soybean root nodule activity was evaluated. Test plots 1 to 6 evaluated are as follows. Test area 1. Control (no application) Test area 2. Ascorbic acid 1,500 ppm foliar spray Test plot 3. Ascorbic acid 3,000 ppm foliar spray Test plot 4. Ascorbic acid 5,000 ppm foliar spray Test plot 5. Ascorbic acid 10,000 ppm foliar spray Test plot 6. Ascorbic acid 20,000ppm foliar spraying
  • Soil preparation and sowing Mix medium-term fertilizing soil (Takii water-containing cell culture medium-term fertilizing type, Takii Seed Co., Ltd.) and fine vermiculite granules (Akagi Gardening Co., Ltd.) at a volume ratio of 1:1, and then mix the soil into the soil. was filled into a polypot (diameter 10.5 cm, height 9 cm).
  • a loopful of the grown rhizobia was taken, inoculated into 50 mL of YM liquid medium in a 500 mL Sakaguchi flask, and cultured with shaking at 30° C. for about 36 hours.
  • a rhizobium culture solution with a turbidity OD600 of about 0.3 was prepared. After sowing, 1 mL of rhizobium culture solution was dropwise inoculated onto the seeds using a micropipettor.
  • Cultivation conditions Cultivation was carried out indoors, with a 16-hour light period, 25° C., an LED light source, and a light intensity of 400 to 440 ⁇ mol/m 2 /s. Seven days after sowing, the plants were thinned to one plant per pot. Watering was carried out by adding enough tap water to the vat to submerge the bottom 5 cm of the pot after the vat placed under the pot had run out of water.
  • Measuring root nodule activity Collection method for exudate to measure the amount of ureide nitrogen, which is an index of nodule activity After 28 days of cultivation, use pruning shears to cut soybean stems into cotyledonous nodes. A 1.5 mL microtube (Eppendorf Co., Ltd.) filled with #10 cotton balls (Kawamoto Sangyo Co., Ltd.) was placed over the stem, and the exudate exuding from the cut surface of the stem was soaked for 2 hours. Recovered. After collecting the exudate, the cotton balls were stored in a -80°C freezer. In addition, the volume of the exudate was calculated by comparing the weight of the cotton balls before and after collecting the exudate.
  • the HPLC device and mass spectrometer were Agilent 1260 Infinity LC system (Agilent Technologies Co., Ltd.) and AB SCIEX TripleQuad 4500 system (AB Sciex Co., Ltd.), respectively. It was used.
  • the column used was Scherzo SS-C18 (100 mm x 2 mm, 3 ⁇ m) (Intact Co., Ltd.), and the oven temperature was 40°C.
  • FIGS. 1 and 2 Results The measurement results of root nodule activity are shown in FIGS. 1 and 2.
  • the graph in the figure represents the mean value ⁇ standard deviation.
  • Figures 1 and 2 there was a tendency for root nodule activity to be higher in test plots with higher ascorbic acid concentrations.
  • the highest nodule activity was observed in test plot 6 where ascorbic acid concentration of 20,000 ppm was sprayed on the leaves.
  • the nodule activity was higher in test plots 4 to 6 compared to test plot 1, indicating that the most preferable ascorbic acid concentration is 5,000 ppm or more.
  • Comparative Example 1 Effect of high concentration ascorbic acid on soybean leaves The effect on soybean leaves when high concentration ascorbic acid was sprayed once on the leaves was evaluated. Test plots 1 and 2 evaluated are as follows. Test area 1. Ascorbic acid 20,000 ppm foliar spray Test plot 2. Ascorbic acid 40,000ppm foliar spraying
  • Results Figure 3 shows a photograph of the plant one week after foliar spraying.
  • the left side of the photo is a soybean whose leaves were sprayed with 40,000 ppm ascorbic acid, and the right side is a soybean whose leaves were sprayed with 20,000 ppm ascorbic acid.
  • After spraying at 40,000 ppm severe problems such as loss of leaf color occurred, indicating that the concentration of ascorbic acid is preferably 20,000 ppm or less.
  • Test plots 1 to 4 evaluated are as follows. Test area 1. Control (no application) Test area 2. Ascorbic acid 300ppm foliar spray Test plot 3. Ascorbic acid 1,500 ppm foliar spray Test plot 4. Ascorbic acid 7,500ppm foliar spray
  • rhizobia Yeast-Mannitol (YM) medium K 2 HPO 4 0.5 g, MgSO 4 .7H 2 O 0.2 g, NaCl 0.1 g, Yeast Extract 0.4 g, Mannitol 10 g, distilled water 1 L (pH 6.8)) and 1.5% agar (Fujifilm Wako Pure Chemical Industries, Ltd.) to prepare a solid medium, and chickpea rhizobium (Mesorhizobium ciceri) strain NBRC100389T was grown on the solid medium.
  • YM rhizobia Yeast-Mannitol
  • a loopful of the grown rhizobia was taken, inoculated into 50 mL of YM liquid medium in a 500 mL Sakaguchi flask, and cultured with shaking at 30° C. for about 48 hours.
  • a rhizobium culture solution with a turbidity OD600 of about 0.3 was prepared. After sowing, 1 mL of rhizobium culture solution was dropwise inoculated onto the seeds using a micropipettor.
  • Cultivation conditions Cultivation was carried out indoors, with a 16-hour light period, 25° C., an LED light source, and a light intensity of 400 to 440 ⁇ mol/m 2 /s. Seven days after sowing, the plants were thinned to one plant per pot. Watering was carried out by adding enough tap water to the vat to submerge the bottom 5 cm of the pot after the vat placed under the pot had run out of water.
  • Test plots 1 to 6 evaluated are as follows. Test area 1. Control (no application) Test area 2. Ascorbic acid 100ppm foliar spray Test plot 3. Ascorbic acid 200ppm foliar spray Test plot 4. Ascorbic acid 500ppm foliar spray test plot 5. Ascorbic acid 1,000 ppm foliar spray test plot 6. Ascorbic acid 2,000ppm foliar spraying
  • a rhizobium culture solution with a turbidity OD600 of about 0.3 was prepared. After sowing, 1 mL of rhizobium culture solution was dropwise inoculated onto the seeds using a micropipettor.
  • Cultivation conditions Cultivation was carried out indoors, with a 16-hour light period, 25° C., an LED light source, and a light intensity of 400 to 440 ⁇ mol/m 2 /s. Seven days after sowing, the plants were thinned to one plant per pot. Watering was carried out by adding enough tap water to the vat to submerge the bottom 5 cm of the pot after the vat placed under the pot had run out of water.

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Abstract

L'invention concerne un procédé et une matière pour accélérer la croissance de germes de soja.　Cet accélérateur d'activité nodulaire pour le soja comprend de l'acide ascorbique ou un sel de celui-ci en tant qu'ingrédient actif. L'accélérateur d'activité nodulaire est appliqué à des germes de soja à une concentration en acide ascorbique de 5 000 à 20 000 ppm en masse
PCT/JP2022/041999 2022-04-28 2022-11-10 Accélérateur d'activité nodulaire WO2023210047A1 (fr)

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Citations (5)

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