WO2019218379A1 - 基于柔性铰链机构的压电超声显微注射装置 - Google Patents

基于柔性铰链机构的压电超声显微注射装置 Download PDF

Info

Publication number
WO2019218379A1
WO2019218379A1 PCT/CN2018/087759 CN2018087759W WO2019218379A1 WO 2019218379 A1 WO2019218379 A1 WO 2019218379A1 CN 2018087759 W CN2018087759 W CN 2018087759W WO 2019218379 A1 WO2019218379 A1 WO 2019218379A1
Authority
WO
WIPO (PCT)
Prior art keywords
flexible hinge
hinge mechanism
cap
device based
cavity
Prior art date
Application number
PCT/CN2018/087759
Other languages
English (en)
French (fr)
Inventor
黄海波
高习玮
陈立国
周飞
郭浩
常凌风
刘吉柱
王阳俊
孙立宁
Original Assignee
苏州大学张家港工业技术研究院
苏州大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 苏州大学张家港工业技术研究院, 苏州大学 filed Critical 苏州大学张家港工业技术研究院
Priority to US16/958,729 priority Critical patent/US11312934B2/en
Publication of WO2019218379A1 publication Critical patent/WO2019218379A1/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure

Definitions

  • the invention relates to the field of microinjection technology, in particular to a piezoelectric ultrasonic microinjection device based on a flexible hinge mechanism.
  • microinjection technology has become an important means of cell engineering research such as transgenic injection, cloning technology, artificial assisted reproductive technology.
  • Membrane rupture technology is the key technology of microinjection. When rupturing the membrane, the injection needle penetrates into the cell body, and then completes the corresponding injection tasks, such as foreign gene injection and nuclear transplantation. During the microinjection, the injection microneedle is in direct contact with the cells, and the quality of the injection device directly affects the activity of the injected cells.
  • the piezoelectric rupture membrane injection method has been widely used in cell microinjection operations as a technique for small cell damage and high injection survival rate.
  • the traditional piezoelectric injection device has the defects of large harmful vibration and complicated operation, which greatly affects the success rate of microinjection.
  • the traditional micro-injector clamping device has poor sealing performance, and most of them adopt spiral seal or ordinary flat washer seal. Under the ultrasonic excitation of piezoelectric ceramic, “leakage” and “leakage” phenomenon are easy to occur, resulting in The experiment produces errors or pollution, which reduces the success rate of the experiment.
  • the object of the present invention is to provide a piezoelectric ultrasonic microinjection device based on a flexible hinge mechanism, which can effectively suppress the harmful radial vibration of the microneedle tip during the microinjection operation and reduce cell damage. Improves the sealing of the device and lays the foundation for safe and efficient microinjection.
  • a piezoelectric ultrasonic microinjection device based on a flexible hinge mechanism comprising:
  • a fixedly assembled closure a flexible hinge mechanism, a base, a screw cap and an end cap, the base having a pump interface;
  • microneedle fixedly mounted in the base, the screw cap and the end cap and extending outward; the microneedle is in communication with the pump interface;
  • the flexible hinge mechanism comprises a housing, a piezoelectric ceramic package module encapsulated in the housing, a central axis fixedly mounted with the piezoelectric ceramic package module and the base, and a vibration output shaft extending from the piezoelectric ceramic package module to the central axis
  • a plurality of flexible hinge beams are disposed between the central shaft and the housing.
  • the end cap and the cap, the cap and the base are respectively fixed by screwing.
  • a gasket is disposed between the end cap and the screw cap and/or between the screw cap and the base, and the two sides of the washer are different first and second tapered surfaces.
  • the end cover comprises an end cap body and an end cap mounting portion, the outer diameter of the end cap main body is larger than the outer diameter of the end cap mounting portion, and the outer side of the end cap mounting portion is provided with the rotation
  • the cover is fixedly mounted with the first thread.
  • the end cap is formed with a first cavity and a second cavity from the end cover main body toward the end cover mounting portion, and the second cavity is tapered.
  • the screw cap includes a screw cap body and a screw cap mounting portion, the outer diameter of the cap cap body is larger than the outer diameter of the cap mounting portion, and the inner side of the cap cap body is provided with the end cap The second thread is fixedly mounted, and the outer side of the cap mounting portion is provided with a third thread fixedly mounted to the base.
  • the spin cover main body in the cap is formed with a third cavity, a fourth cavity and a fifth cavity toward the cap mounting portion, and the fifth cavity is tapered.
  • the base comprises a bent section fixedly mounted with the screw cap, and a horizontal section fixedly mounted with the flexible hinge mechanism, the bent section and the horizontal section passing through the thread and the screw cap and the flexible hinge mechanism Fixed installation separately.
  • the inner side of the bent section is provided with a fourth thread fixedly mounted to the screw cap.
  • a sixth cavity and a seventh cavity are disposed inside the bending section, and an eighth cavity is disposed inside the horizontal section, and the seventh cavity is tapered, and the sixth cavity The body communicates with the pump interface through the seventh cavity and the micro flow channel.
  • the inner side of the horizontal section is provided with a fifth thread
  • the outer side of the central end of the flexible hinge mechanism is provided with a sixth thread
  • the base and the flexible hinge mechanism pass the fifth thread and the sixth thread Fixed installation.
  • the end of the horizontal section is provided with a first flange, and a plurality of first reinforcing ribs are disposed between the first flange and the horizontal section.
  • the flexible hinge mechanism has a second flange disposed on a central axis thereof, and a plurality of second reinforcing ribs are disposed between the second flange and the central shaft.
  • the flexible hinge beam is provided with a plurality of V-shaped recesses.
  • the flexible hinge beam is an equal-angle 120° circumferential array segment centered on the central axis, and the flexible hinge beams in each direction are equidistant double flexible hinges at a certain distance in the axial direction. Beam parallel linear array distribution.
  • the central shaft is provided with a mounting hole, and a screw is fixedly mounted in the mounting hole to fix the central axis and the vibration output shaft.
  • a three-dimensional flexible hinge mechanism is designed for the piezoelectric ceramic package module, which can effectively filter and buffer the vibration output while maintaining high energy transmission efficiency. Radial harmful vibration of the shaft, thereby reducing lateral harmful vibrations of the microneedle tip;
  • the double-cone shaped shaped washer self-sealing mechanism effectively realizes the functions of air sealing and liquid sealing, and the mechanism has better clamping and stabilizing effect on the micro needle;
  • the "flexible hinge mechanism-base” ultrasonic energy transfer connection is optimized, that is, the flange surface contact and the rib design are adopted, and the overall quality of the mechanism is greatly reduced and the overall quality of the mechanism is greatly improved without impairing the overall strength and function of the microneedle.
  • the energy transfer efficiency of piezoelectric ceramics is optimized, that is, the flange surface contact and the rib design are adopted, and the overall quality of the mechanism is greatly reduced and the overall quality of the mechanism is greatly improved without impairing the overall strength and function of the microneedle.
  • FIG. 1 is a schematic perspective view showing a piezoelectric ultrasonic microinjection device according to an embodiment of the present invention
  • FIG. 2 is a front elevational view showing a piezoelectric ultrasonic microinjection device according to an embodiment of the present invention
  • FIG. 3 is a schematic cross-sectional structural view of a piezoelectric ultrasonic microinjection device according to an embodiment of the present invention.
  • FIG. 4 is a schematic perspective view of an end cover according to an embodiment of the present invention.
  • FIG. 5 is a schematic cross-sectional structural view of an end cap according to an embodiment of the present invention.
  • FIG. 6 is a schematic perspective view of a screw cap according to an embodiment of the present invention.
  • FIG. 7 is a schematic cross-sectional structural view of a screw cap according to an embodiment of the present invention.
  • FIG. 8 is a schematic perspective structural view of a susceptor according to an embodiment of the present invention.
  • FIG. 9 is a schematic cross-sectional structural view of a susceptor according to an embodiment of the present invention.
  • 10a is a schematic perspective view of a gasket in an embodiment of the present invention.
  • Figure 10b is a cross-sectional structural view showing a gasket in an embodiment of the present invention.
  • 11a is a schematic perspective view of a flexible hinge mechanism according to an embodiment of the present invention.
  • 11b is a front elevational view showing the flexible hinge mechanism according to an embodiment of the present invention.
  • FIG. 12 is a schematic view showing the assembly structure of a flexible hinge mechanism according to an embodiment of the present invention.
  • FIG. 13 is a schematic view showing another angle assembly structure of the flexible hinge mechanism according to an embodiment of the present invention.
  • FIG. 1 a specific embodiment of the present invention discloses a piezoelectric ultrasonic microinjection device based on a flexible hinge mechanism, which mainly comprises a microneedle.
  • a fixed assembly cover 10 a flexible hinge mechanism 20, a base 30, a screw cap 40 and an end cover 50, the base 30 is provided with a pump interface 35;
  • microneedle 60 fixedly mounted in the base, the screw cap and the end cap and extending outward; the microneedle 60 is in communication with the pump interface 35;
  • the flexible hinge mechanism 20 includes a housing 21, a piezoelectric ceramic package module 22 encapsulated in the housing, a central pivot 23 fixedly mounted to the piezoelectric ceramic package module and the base, and extending from the piezoelectric ceramic package module 22 into the central shaft 23 A plurality of flexible hinge beams 25 are disposed between the vibration output shaft 24 and the central shaft 23 and the housing 21.
  • end cap 50 and the screw cap 40, the screw cap 40 and the base 30 are respectively fixed by screwing.
  • the end cover 50 includes an end cover main body 51 and an end cover mounting portion 52.
  • the outer diameter of the end cover main body 51 is larger than the outer diameter of the end cover mounting portion 52, and the outer side of the end cover mounting portion 52 is provided.
  • the first thread 521 is fixedly mounted with the screw cap.
  • a first cavity 501 and a second cavity 502 are formed in the end cover 50 from the end cover main body 51 toward the end cover mounting portion 52, and the second cavity 502 is tapered.
  • the end cover 50 in the embodiment is a second-order stepped shaft-shaped part, and has an external thread on one side of the small cylindrical surface, and a tapered hole on the end surface of the small cylindrical surface, and penetrates the entire part at the same time.
  • the axial position is provided with a fully penetrating light face having an inner diameter of 1.2 mm which is slightly larger than the outer diameter of the microneedle 60 by 1 mm for the purpose of facilitating the mounting of the microneedles.
  • the screw cap 40 includes a cap body 41 and a cap mounting portion 42.
  • the outer diameter of the cap main body 41 is larger than the outer diameter of the cap mounting portion 42, and the cap body 41 is provided.
  • the inner side is provided with a second thread 411 fixedly mounted to the end cover 50, and the outer side of the cap mounting portion 42 is provided with a third thread 421 fixedly mounted to the base 30.
  • a third cavity 401, a fourth cavity 402, and a fifth cavity 403 are formed in the spin cover 40 in the direction of the cap mounting portion, and the fifth cavity 403 is tapered.
  • the screw cap 40 of the present embodiment is matched with the end cover 50.
  • the screw cap 40 is also a second-step stepped shaft-shaped component, and the large-diameter end of the screw cap 40 is provided with an internal thread matched with the end cap 50, and The thread length is slightly less than the outer thread length of the end cap 50 in order to fully screw the end cap 50 into the internal thread of the screw cap 40.
  • the end surface of the screw cap 40 is provided with a tapered hole surface, and the large diameter of the tapered surface is just the outer diameter of the internal thread.
  • the outer cylindrical surface of the other end (small diameter) of the screw cap 40 is provided with an external thread.
  • the external thread size parameter is the same as the external thread size parameter of the end cap 50.
  • the screw cap 40 is provided with a tapered hole at the end face of the external thread, and the dimensional parameters thereof are the same as those of the tapered cap of the end cap 50.
  • a through hole having an inner diameter of 1.2 mm is also formed in the central axis.
  • the base 30 includes a bent section 31 fixedly mounted to the screw cap 40, and a horizontal section 32 fixedly mounted to the flexible hinge mechanism 20, the bent section 31 and the horizontal section 32. It is fixedly mounted by screws, screw caps and flexible hinge mechanisms.
  • a fourth thread (not shown) fixedly mounted to the screw cap is disposed on the inner side of the bending section 31.
  • the sixth cavity 3011 and the seventh cavity 3012 are disposed inside the bending section 31, and the eighth section is disposed inside the horizontal section 32.
  • the cavity 304, the seventh cavity 3012 is tapered, and the sixth cavity 3011 is in communication with the pump interface 35 through the seventh cavity 3012 and the micro flow channel 302.
  • the inner side of the horizontal section 32 is provided with a fifth thread (not shown), the outer side of the central end of the flexible hinge mechanism 20 is provided with a sixth thread, and the base 30 and the flexible hinge mechanism 20 are fixedly mounted by the fifth thread and the sixth thread. .
  • the end of the horizontal section 32 is provided with a first flange 33, and a plurality of first reinforcing ribs 34 are disposed between the first flange 33 and the horizontal section 32.
  • the base 30 is divided into two sections, and is divided into a bending section 31 and a horizontal section 32 during installation.
  • the bending section 31 is provided with an internal thread matched with the external thread of the screw cap 40, and the thread length is slightly smaller than the external thread length of the screw cap 40, in order to completely screw the screw cap 40 into the internal thread of the base 30.
  • a tapered face is formed at the end face of the screw in the base 30, and its size is the same as that of the tapered face at the end face of the screw cap 40.
  • the horizontal section 32 is internally provided with a microchannel 302 extending from the upper end pump port 35 to the end face of the bent portion 32. As shown in FIG.
  • the end surface of the horizontal section of the base 30 is provided with internal threads to cooperate with the external threads of the central shaft of the flexible hinge mechanism 20.
  • the flexible hinge mechanism 20 filters the ultrasonic energy generated by the piezoelectric ceramic package module 22 through the flexible hinge and transmits it to the susceptor 30 through the vibration output shaft 24.
  • a gasket 80 is disposed between the end cover 50 and the screw cap 40 and between the screw cap 40 and the base 30 , and the first side of the washer 80 is different. Face 81 and second tapered surface 82.
  • both ends of the washer 80 are tapered surfaces and are divided into a first tapered surface 81 and a second tapered surface 82.
  • the gasket 80 has a through hole at a central axis position which is exactly the outer diameter of the microneedle 60.
  • the washer 80 is first loaded into the bottom taper face of the internal thread of the base 30, the first tapered surface 81 is mated with the tapered face of the base 30, and then screwed into the screw cap 40, but not tightened.
  • a washer 80 is placed on the bottom of the inner screw of the screw cap 40, the first tapered surface 81 of the washer 80 is fitted thereto, and then the end cap 50 is screwed, and also not tightened.
  • microneedle 60 is adjusted to be properly inserted from the end face of the first tapered surface 81 of the end cap 50 until the end face of the microneedle 60 is brought to the position of the microchannel 302 of the base 30 to stop. Finally, the screw cap 40 and the end cap 50 are tightened at the same time until the screwing to the limit position cannot continue to rotate.
  • the second tapered surface 82 of the washer 80 engages with the tapered face at the outer thread end face of the screw cap 40, and the tapered surface of the washer 80 is slightly larger in size than the corresponding mating cone face.
  • the purpose of the gasket 80 is to: in the process of screwing the end cap 50 into the threaded hole of the screw cap 40 due to the elastic deformation characteristic of the gasket 80, the first tapered surface 81 of the gasket 80 is subjected to the tightening force to the bottom of the threaded bottom of the base 30.
  • the tapered face wall is pressed, and at the same time, the reaction force of the washer 80 causes the second tapered surface 82 of the washer 80 to be pressed against the tapered face wall at the external thread of the screw cap 40, thereby achieving a self-sealing effect.
  • the taper surface of the washer 80 receives the tightening force, mechanically decomposed, due to the force of the tapered surface, a radial force is generated on the surface of the pinhole wall of the microneedle 60, so that the cylindrical bore in the washer 80 during the tightening process
  • the outer cylindrical surface of the wall and microneedle 60 achieves an automatic sealing effect.
  • the washer 80 reaches the limit of elastic deformation, thereby forming an extrusion with the inner wall, and forming a multi-directional multi-angle self-sealing environment through the surface contact.
  • the first tapered surface 81 of the washer 80 also presses against the tapered face wall at the bottom of the threaded hole in the screw cap 40, and is also subjected to a reaction force.
  • the second tapered surface 82 of the gasket 80 presses the taper face of the externally threaded end face of the end cap 50, and the inner wall of the gasket 80 presses the outer wall of the microneedle 60 to achieve a self-sealing effect.
  • the design of the two needles for the clamping of the microneedles 60 can effectively avoid the radial vibration of the microneedles 60, and can transmit the axial vibration transmitted by the vibration output shaft 24 of the flexible hinge mechanism more efficiently.
  • the gasket 80 and the microneedle 60 used in this time are in surface contact, the design of the gasket 80 can better fix the microneedle 60 and prevent the microneedle 60 from being compared with the conventional point contact and hard contact.
  • the relative displacement with the clamping mechanism reduces mechanical wear between the microneedle 60 and the clamping cap 40, the base 30 and the end cap 50. Therefore, the design of the gasket 80 and the corresponding base 30, the screw cap 40, and the tapered surface of the end cover 50 not only can achieve a better sealing effect, but also has a more stable and firm clamping and fixing of the microneedle 60. The effect does not cause problems such as "radial rotation” and "axial slip" of the microneedle 60 during use.
  • the first flange 33 is disposed on the end surface of the horizontal section 32 of the base 30, and the second flange 26 is disposed on the central shaft 23 of the flexible hinge mechanism 20.
  • the first flange 33 and the second flange 26 are correspondingly disposed, and the second flange 26 is provided with a threaded hole at the end surface thereof, and the threaded hole is exactly the same as the outer diameter of the end surface of the central shaft 23 of the flexible hinge mechanism, and the second flange 26
  • the threaded bore of the end face cooperates with the externally threaded section of the central axle 23 of the flexible hinge structure.
  • the central shaft 23 of the flexible hinge mechanism is completely screwed into the threaded hole in the end surface of the first flange 33 of the base 30 until the end surface of the central shaft 23 of the flexible hinge mechanism 20 and the first flange of the horizontal section of the base 30 33 end face fit to achieve surface contact.
  • the first ribs are sequentially disposed in the "3 o'clock”, “6 o'clock”, “9 o'clock”, and “12 o'clock” directions between the first flange 33 and the outer cylinder of the horizontal section of the pedestal 30.
  • the purpose is to enhance the structural strength of the base 30 when the vibration output shaft 24 of the flexible hinge mechanism 20 transmits the high frequency vibration to the base 30 through the pivot shaft 23, and at the same time, to reduce its own weight and improve the energy transfer efficiency. .
  • the flexible hinge mechanism 20 is a single piece, the housing 21 is a cylindrical housing, and the central shaft 23 and the inner wall of the housing 21 are connected by a flexible hinge beam 25,
  • the flexible hinge beam 25 is a "V" type flexible hinge beam having a plurality of V-shaped recesses thereon.
  • the flexible hinge beam has a circumferential array of 120° at an angle of 0°, 120°, 240° in the end direction of the central axis 23, and the flexible beams in each direction are separated by a certain distance in the axial direction. Parallel linear array distribution from double flexible beams. Therefore, a total of six flexible hinge beams 25 are connected to the housing 21 on the center shaft 23.
  • V type flexible hinge beam design has the best axial vibration transmission efficiency, and the filtering effect on the radial residual vibration is also the best.
  • a threaded hole is provided at the end surface of the center shaft 23 to cooperate with the external thread of the vibration output shaft 24 of the piezoelectric ceramic package module 22.
  • a plurality of second reinforcing ribs 27 are provided between the second flange 26 and the center shaft 23. The position is in the middle of three flexible hinge beams 25, and is also distributed in an array of 120° centered on the axis.
  • the cover 10 is a second-order stepped shaft part, and an external thread (not shown) is provided at a small diameter section of the cover 10, and the large-diameter end face of the cover 10 is provided. Smooth through hole.
  • the end of the inner wall end of the end portion of the flexible hinge mechanism 20 is provided with an internal thread which is matched with the external thread at the small shaft end of the cover 10.
  • the fixing rod 70 is also a second-order stepped shaft part, and the cylindrical surface of the small-axis diameter section of the end face is provided with an external thread.
  • the assembly process of the flexible hinge mechanism 20, the piezoelectric ceramic package module 22, the cover 10, the fixing rod 70, and the set screw 28 firstly screw the vibration output shaft 24 of the piezoelectric ceramic package module 22 into the pivot of the flexible hinge mechanism 20 In the threaded hole at the end face of the 23, the set screw 28 is then screwed into the mounting hole 231 on the cylindrical surface of the central shaft 23, so that the head of the set screw 28 abuts against the external thread surface of the vibration output shaft 24, thereby relaxing and reinforcing. .
  • the small shaft end external threads of the closure 10 are then mated with the internal threads at the end faces of the flexible hinge mechanism 20.
  • the small shaft end of the fixing rod 70 is inserted into the smooth surface through hole of the large shaft end surface of the cover 10, and the external thread of the small shaft end of the fixing rod 70 is screwed into the internal thread at the end surface of the piezoelectric ceramic package module 22.
  • the end surface of the piezoelectric ceramic package module 22 and the end surface of the small diameter section of the cover 10 are continuously fitted and tightened, and the piezoelectric ceramic package module 22 is also driven to rotate, so that the piezoelectric ceramic package module 22 vibrates.
  • the output shaft 24 is continuously tightened and fixed with the threaded hole of the pivot shaft 23 of the flexible hinge mechanism 20, and the stepped shaft end surface of the fixing rod 70 and the end surface of the large shaft diameter section of the cover 10 are tightly fitted, thereby realizing the piezoelectric ceramic package module 22 and the fixed rod. 70. Assembly and fastening process of the cover 10 and the flexible hinge mechanism housing 21.
  • the working principle of the piezoelectric ultrasonic microinjection device based on the flexible hinge mechanism in the present invention is as follows:
  • the fixed rod 70 is fixed to the frame and remains absolutely stationary relative to the static ground.
  • the head end thread of the fixing rod 70 is screwed through the through hole of the cover 10 into the threaded hole of the end surface of the piezoelectric ceramic package module 22, and the cover 10 and the piezoelectric ceramic package module 22 and the fixing rod 70 are fixed by screwing.
  • the flexible hinge mechanism housing 21 is also fixed by the screw connection and the cover 10, so the flexible hinge mechanism housing 21, the cover 10, the fixing rod 70, and the piezoelectric ceramic package module 22 are all fixed ends.
  • the vibration output shaft 24 After the piezoelectric ceramic package module 22 is connected to the power source, the vibration output shaft 24 generates high-frequency vibration, and the vibration is transmitted to the central axis 23 of the flexible hinge mechanism 20 through the screw connection between the vibration output shaft 24 and the center shaft 23. Since the piezoelectric ceramic package module 22 generates a small amount of irregular radial vibration during actual motion. If the energy is directly transmitted to the susceptor 30 without any filtering buffer, the radial residual vibration will be amplified to a large lateral vibration of the needle tip after being transported over a long distance to the needle tip of the microneedle 60. Conducive to cell microinjection and membrane rupture.
  • the central axis can only be displaced along the axial direction due to the restraining action of the three-way flexible hinge, and any radial residual vibration will be absorbed by the three-way flexible hinge beam. Therefore, the central axis, the ribs, the flanges and the output shaft of the flexible hinge mechanism are integral, all of which are movable ends, and the whole is linearly high-frequency reciprocating with respect to the fixed end section (stationary reference). The central axis finally transmits the filtered ultrasonic vibration energy to the vibration output shaft, and finally transmits the vibration energy to the base through the screw connection of the vibration output shaft and the base.
  • a three-dimensional flexible hinge mechanism is designed for the piezoelectric ceramic package module, which can effectively filter and buffer the vibration output while maintaining high energy transmission efficiency. Radial harmful vibration of the shaft, thereby reducing lateral harmful vibrations of the microneedle tip;
  • the double-cone shaped shaped washer self-sealing mechanism effectively realizes the functions of air sealing and liquid sealing, and the mechanism has better clamping and stabilizing effect on the micro needle;
  • the "flexible hinge mechanism-base” ultrasonic energy transfer connection is optimized, that is, the flange surface contact and the rib design are adopted, and the overall quality of the mechanism is greatly reduced and the overall quality of the mechanism is greatly improved without impairing the overall strength and function of the microneedle.
  • the energy transfer efficiency of piezoelectric ceramics is optimized, that is, the flange surface contact and the rib design are adopted, and the overall quality of the mechanism is greatly reduced and the overall quality of the mechanism is greatly improved without impairing the overall strength and function of the microneedle.
  • the present invention can meet bioengineering micromanipulation experiments of different needs, such as: nuclear transfer, single sperm injection, transgenic injection, and the like.
  • the microneedle clamping section adopts the rib design, the overall quality of the microinjector is greatly reduced, and the three-dimensional flexible hinge mechanism is used to effectively reduce the radial vibration of the microneedle tip caused by the piezoelectric ceramic, thereby Effectively alleviate the mechanical damage caused by injection to the cells and improve the success rate of the experiment.
  • the invention has the advantages of simple structure, good interchangeability of components, easy assembly, and relatively low processing cost, which simplifies the experimental cost and complexity of the microinjection experiment operation.

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Mechanical Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • General Electrical Machinery Utilizing Piezoelectricity, Electrostriction Or Magnetostriction (AREA)
  • Surgical Instruments (AREA)

Abstract

一种基于柔性铰链机构的压电超声显微注射装置，包括：固定装配的封盖（10）、柔性铰链机构（20）、基座（30）、旋盖（40）及端盖（50），所述基座（30）上设有泵接口（35）；固定安装于基座（30）、旋盖（40）及端盖（50）内且向外延伸的微针（60），所述微针（60）与泵接口（35）相连通；所述柔性铰链机构（20）包括壳体（21）、封装于壳体（21）内的压电陶瓷封装模块（22）、与压电陶瓷封装模块（22）及基座（30）固定安装的中枢轴（23）、自压电陶瓷封装模块（22）延伸至中枢轴（23）内的振动输出轴（24），所述中枢轴（23）与壳体（21）之间设有若干柔性铰链梁（25）。该装置可有效的抑制显微注射操作过程中的微针针尖有害径向振动，减小细胞损伤，大幅提高了装置的密封性。

Description

基于柔性铰链机构的压电超声显微注射装置 技术领域
本发明涉及显微注射技术领域，特别是涉及一种基于柔性铰链机构的压电超声显微注射装置。
背景技术
随着生物技术的快速发展，显微注射技术已经成为诸如转基因注射、克隆技术、人工辅助生殖技术等细胞工程研究的重要手段。破膜技术是显微注射的关键技术，破膜穿刺时注射针穿入细胞体内，然后完成相应的注射任务，如外源基因注射、核移植等。在显微注射过程中，注射微针与细胞直接接触，注射装置的好坏将直接影响被注射细胞的活性。
压电破膜注射方法作为一种对细胞损害小、注射成活率高的技术已经广泛的应用于细胞显微注射操作中。但传统压电注射装置存在有害振动大、操作复杂等缺陷，极大的影响了显微注射成功率。另外，传统的显微注射器的夹持装置密封性性能较差，大都采用螺旋密封或普通平垫圈密封，在压电陶瓷的超声激励下容易发生“漏气”、“漏液”现象，从而导致实验产生误差或造成污染，降低了实验的成功率。
因此，针对上述技术问题，有必要提供一种基于柔性铰链机构的压电超声显微注射装置。
发明内容
有鉴于此，本发明的目的在于提供一种基于柔性铰链机构的压电超声显 微注射装置，可有效的抑制显微注射操作过程中的微针针尖有害径向振动，减小细胞损伤，大幅提高了装置的密封性，为实现安全、高效的显微注射奠定基础。
为了实现上述目的，本发明一实施例提供的技术方案如下：
一种基于柔性铰链机构的压电超声显微注射装置，所述装置包括：
固定装配的封盖、柔性铰链机构、基座、旋盖及端盖，所述基座上设有泵接口；
固定安装于基座、旋盖及端盖内且向外延伸的微针，所述微针与泵接口相连通；
所述柔性铰链机构包括壳体、封装于壳体内的压电陶瓷封装模块、与压电陶瓷封装模块及基座固定安装的中枢轴、自压电陶瓷封装模块延伸至中枢轴内的振动输出轴，所述中枢轴与壳体之间设有若干柔性铰链梁。
作为本发明的进一步改进，所述端盖与所述旋盖、所述旋盖与所述基座之间分别通过螺纹固定安装。
作为本发明的进一步改进，所述端盖与旋盖之间和/或旋盖与基座之间设有垫圈，所述垫圈的两侧为不同的第一锥面和第二锥面。
作为本发明的进一步改进，所述端盖包括端盖主体及端盖安装部，所述端盖主体的外径大于端盖安装部的外径，所述端盖安装部的外侧设有与旋盖固定安装的第一螺纹。
作为本发明的进一步改进，所述端盖内自端盖主体向端盖安装部方向形成有第一腔体和第二腔体，所述第二腔体呈锥形。
作为本发明的进一步改进，所述旋盖包括旋盖主体及旋盖安装部，所述旋盖主体的外径大于旋盖安装部的外径，所述旋盖主体的内侧设有与端盖固定安装的第二螺纹，所述旋盖安装部的外侧设有与基座固定安装的第三螺纹。
作为本发明的进一步改进，所述旋盖内自旋盖主体向旋盖安装部方向形 成有第三腔体、第四腔体和第五腔体，所述第五腔体呈锥形。
作为本发明的进一步改进，所述基座包括与旋盖固定安装的折弯段、以及与柔性铰链机构固定安装的水平段，所述折弯段及水平段通过螺纹与旋盖及柔性铰链机构分别固定安装。
作为本发明的进一步改进，所述折弯段的内侧设有与旋盖固定安装的第四螺纹。
作为本发明的进一步改进，所述折弯段内部设有第六腔体及第七腔体，水平段内部设有第八腔体，所述第七腔体呈锥形，所述第六腔体通过第七腔体及微流道与泵接口相连通。
作为本发明的进一步改进，所述水平段的内侧设有第五螺纹，柔性铰链机构的中枢轴端部外侧设有第六螺纹，所述基座与柔性铰链机构通过第五螺纹和第六螺纹固定安装。
作为本发明的进一步改进，所述水平段的端部设有第一法兰，第一法兰和水平段之间设有若干第一加强筋。
作为本发明的进一步改进，所述柔性铰链机构的中枢轴上设有第二法兰，所述第二法兰与中枢轴之间设有若干第二加强筋。
作为本发明的进一步改进，所述柔性铰链梁上设有若干V型凹陷部。
作为本发明的进一步改进，所述柔性铰链梁以中枢轴轴心为中心等角度120°圆周阵列分部，且每个方向的柔性铰链梁均在轴向方向相隔一定距离呈等距双柔性铰链梁平行线性阵列分布。
作为本发明的进一步改进，所述中枢轴上设有安装孔，安装孔内固定安装有螺钉，以固定所述中枢轴和振动输出轴。
本发明的有益效果是：
针对压电陶瓷封装模块传导到微针针尖的有害径向振动，对压电陶瓷封装模块设计了一种三维立体柔性铰链机构，在保持高能量传输效率的同时， 也能有效过滤、缓冲振动输出轴的径向有害振动，从而减少微针针尖的横向有害振动；
双锥面异形垫圈自密封机构有效的实现了气密封和液密封的功能，同时该机构对微针具有更好的夹持稳固作用；
对“柔性铰链机构-基座”超声能量传递连接部分进行优化，即采用法兰盘面接触和加强筋的设计，在不削弱微针整体强度和功能的前提下，大大减轻了机构整体质量，提高了压电陶瓷的能量传递效率。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明中记载的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1为本发明一具体实施方式中压电超声显微注射装置的立体结构示意图；
图2为本发明一具体实施方式中压电超声显微注射装置的正视结构示意图；
图3为本发明一具体实施方式中压电超声显微注射装置的剖面结构示意图；
图4为本发明一具体实施方式中端盖的立体结构示意图；
图5为本发明一具体实施方式中端盖的剖面结构示意图；
图6为本发明一具体实施方式中旋盖的立体结构示意图；
图7为本发明一具体实施方式中旋盖的剖面结构示意图；
图8为本发明一具体实施方式中基座的立体结构示意图；
图9为本发明一具体实施方式中基座的剖面结构示意图；
图10a为本发明一具体实施方式中垫圈的立体结构示意图；
图10b为本发明一具体实施方式中垫圈的剖视结构示意图；
图11a为本发明一具体实施方式中柔性铰链机构的立体结构示意图；
图11b为本发明一具体实施方式中柔性铰链机构的正视结构示意图；
图12为本发明一具体实施方式中柔性铰链机构的装配结构示意图；
图13为本发明一具体实施方式中柔性铰链机构的另一角度装配结构示意图。
具体实施方式
为了使本技术领域的人员更好地理解本发明中的技术方案，下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都应当属于本发明保护的范围。
参图1、图2、图11a、图11b、图12、图13所示，本发明一具体实施方式中公开了一种基于柔性铰链机构的压电超声显微注射装置，其主要包括微针夹持及自密封模块、柔性铰链过滤机构及能量传递机构两大部分，具体包括：
固定装配的封盖10、柔性铰链机构20、基座30、旋盖40及端盖50，基座30上设有泵接口35；
固定安装于基座、旋盖及端盖内且向外延伸的微针60，微针60与泵接口35相连通；
柔性铰链机构20包括壳体21、封装于壳体内的压电陶瓷封装模块22、 与压电陶瓷封装模块及基座固定安装的中枢轴23、自压电陶瓷封装模块22延伸至中枢轴23内的振动输出轴24，中枢轴23与壳体21之间设有若干柔性铰链梁25。
本实施例中的端盖50与旋盖40、旋盖40与基座30之间分别通过螺纹固定安装。
参图3-图5所示，端盖50包括端盖主体51及端盖安装部52，端盖主体51的外径大于端盖安装部52的外径，端盖安装部52的外侧设有与旋盖固定安装的第一螺纹521。端盖50内自端盖主体51向端盖安装部52方向形成有第一腔体501和第二腔体502，第二腔体502呈锥形。
具体地，本实施方式中的端盖50为一个二阶阶梯轴形零件，在小圆柱面一侧设有外螺纹，同时在小圆柱面端面设有锥形孔，同时贯穿整个零件，在中轴线位置设有完全贯穿的光面孔，其内径为1.2mm，略微大于微针60的外径1mm，目的是为了便于微针的安装。
参图1-3、图6、图7所示，旋盖40包括旋盖主体41及旋盖安装部42，旋盖主体41的外径大于旋盖安装部42的外径，旋盖主体41的内侧设有与端盖50固定安装的第二螺纹411，旋盖安装部42的外侧设有与基座30固定安装的第三螺纹421。旋盖40内自旋盖主体向旋盖安装部方向形成有第三腔体401、第四腔体402和第五腔体403，第五腔体403呈锥形。
具体地，本实施方式中的旋盖40与端盖50相配合，旋盖40同样为一个二阶阶梯轴状零件，旋盖40大直径一端设有与端盖50相配合的内螺纹，且螺纹长度略小于端盖50外螺纹长度，目的是为了能将端盖50完全旋进旋盖40的内螺纹中去。如图7所示，旋盖40内螺纹端面处设有一锥形孔面，其锥面大径正好为内螺纹外径。旋盖40另一端(小直径)外圆柱面设有外螺纹。其外螺纹尺寸参数与端盖50外螺纹尺寸参数相同。另外，与端盖50设计类似，旋盖40在外螺纹端面处设有锥形孔，其尺寸参数与端盖50锥形孔参数 相同。同时，为了微针60的贯穿安装，同样在其中轴线上也开有内径为1.2mm的贯穿孔。
参图1-3、图6-9所示，基座30包括与旋盖40固定安装的折弯段31、以及与柔性铰链机构20固定安装的水平段32，折弯段31及水平段32通过螺纹与旋盖及柔性铰链机构分别固定安装。
折弯段31的内侧设有与旋盖固定安装的第四螺纹(未图示)，折弯段31内部设有第六腔体3011及第七腔体3012，水平段32内部设有第八腔体304，第七腔体3012呈锥形，第六腔体3011通过第七腔体3012及微流道302与泵接口35相连通。
水平段32的内侧设有第五螺纹(未图示)，柔性铰链机构20的中枢轴端部外侧设有第六螺纹，基座30与柔性铰链机构20通过第五螺纹和第六螺纹固定安装。
另外，水平段32的端部设有第一法兰33，第一法兰33和水平段32之间设有若干第一加强筋34。
具体地，基座30一共分为两段，安装时分为折弯段31和水平段32。折弯段31设有与旋盖40外螺纹相配套的内螺纹，螺纹长度略小于旋盖40外螺纹长度，目的是为了使旋盖40完全拧入基座30内螺纹中去。同样，在基座30内螺纹端面处开有一锥面孔，其尺寸和旋盖40内螺纹端面处的锥面孔尺寸相同。另外，如图9所示，水平段32内部设有微通道302，从上端泵接口35处一直贯穿至折弯段32内螺纹端面处。如图3所示，基座30水平段的端面处设有内螺纹，与柔性铰链机构20的中枢轴的外螺纹相配合。柔性铰链机构20将压电陶瓷封装模块22产生的超声能量经柔性铰链过滤缓冲后通过振动输出轴24传递给基座30。
参图1、图3、图10a、图10b所示，端盖50与旋盖40之间和旋盖40与基座30之间设有垫圈80，垫圈80的两侧为不同的第一锥面81和第二锥面 82。
具体地，垫圈80的两端均为锥面，分为第一锥面81和第二锥面82。垫圈80在中轴线位置开有贯穿孔，其尺寸正好为微针60的外直径。在装配过程中，首先将垫圈80装入基座30的内螺纹底部锥面孔处，将第一锥面81与基座30的锥面孔相配合，然后旋入旋盖40，但不要拧紧。同理，在旋盖40内螺纹底部放置一个垫圈80，将垫圈80的第一锥面81与之配合，之后再拧入端盖50，同样不要拧紧。最后，再将微针60调节好适当位置从端盖50的第一锥面81的端面孔中插入，直到将微针60的端面抵到基座30的微流道302位置处停止。最后，再同时拧紧旋盖40和端盖50，直到拧到极限位置无法继续旋转为止。垫圈80的第二锥面82和旋盖40外螺纹端面处的锥面孔配合，且垫圈80锥面的尺寸略大于相应配合锥面孔的尺寸。
垫圈80设计的目的在于：由于垫圈80具有弹性变形特性，将端盖50旋入旋盖40内螺纹孔的过程中，垫圈80的第一锥面81受拧紧力会对基座30内螺纹底部的锥面孔壁形成挤压，同时，垫圈80受反作用力会使得垫圈80的第二锥面82对旋盖40外螺纹处的锥面孔壁形成挤压，从而达到自密封的效果。
另外，由于垫圈80锥面收到拧紧力，经力学分解，由于锥面受力的特性，会同时对微针60针壁表面产生一个径向力，使得在拧紧过程中，垫圈80内圆柱孔壁和微针60外圆柱面达到一个自动密封的效果。且当实验人员将旋盖40拧紧时，垫圈80达到弹性变形的极限，从而和内壁形成挤压，通过面接触形成多方向多角度的自密封环境。同理，在端盖50旋入旋盖40内螺纹过程中，垫圈80的第一锥面81也会对旋盖40内螺纹孔底部的锥面孔壁形成挤压，同时，受到反作用力的作用，垫圈80的第二锥面82会对端盖50的外螺纹端面锥面孔形成挤压，以及垫圈80内孔壁对微针60外壁进行挤压，从而达到自密封的效果。
参图3所示，由于微针60在进行细胞破膜时，需要在压电陶瓷封装模块22高频振动下才能完成显微操作。因此，对于微针60的夹持采用两点支撑的设计能有效避免微针60的径向振动，能更加高效的传递由柔性铰链机构的振动输出轴24传递过来轴向振动。另外，由于本次采用的垫圈80与微针60为面接触，相比传统的点接触和硬接触，垫圈80的设计能对微针60起到更好的固定夹持作用，防止微针60和夹持机构发生相对位移，从而减少了微针60与夹持旋盖40、基座30及端盖50的之间的机械磨损。因此，该垫圈80和对应基座30、旋盖40、端盖50锥面孔相配合的设计不仅能起到更好密封效果，同时该设计能对微针60具有更稳定、牢固的夹持固定效果，不会造成微针60在使用过程中“径向转动”和“轴向滑移”等问题的出现。
参图3、图8、图11a、图11b、图12所示，基座30的水平段32端面设有第一法兰33，柔性铰链机构20的中枢轴23上设有第二法兰26，第一法兰33和第二法兰26相对应设置，第二法兰26端面处设有螺纹孔，螺纹孔恰好与柔性铰链机构的中枢轴23端面外圆直径相同，第二法兰26端面的螺纹孔与柔性铰链结构的中枢轴23的外螺纹段相配合。实际装配过程中，柔性铰链机构的中枢轴23完全拧入基座30上第一法兰33端面内螺纹孔中，直到柔性铰链机构20的中枢轴23端面和基座30水平段第一法兰33端面配合，实现面接触。
如图8所示，在第一法兰33和基座30水平段外圆柱体间的“3点”、“6点”、“9点”、“12点”方向依次设有第一加强筋，目的在于柔性铰链机构20的振动输出轴24将高频振动通过中枢轴23传递给基座30时，起到加强基座30的结构强度作用，同时也为了降低其自身重量，提高能量传递效率。
如图3、图11a、图11b、图12所示，柔性铰链机构20为一个整体件，壳体21为圆柱壳体，中枢轴23与壳体21内壁之间通过柔性铰链梁25相连接，柔性铰链梁25为“V”型柔性铰链梁，其上设有若干V型凹陷部。柔性 铰链梁以中枢轴23轴心为中心在端面方向的0°、120°、240°位置等角度120°圆周阵列分部，且每个方向的柔性梁均在轴向方向相隔一定距离呈等距双柔性梁平行线性阵列分布。因此，在中枢轴23上，共有6根柔性铰链梁25与壳体21连接。
通过理论计算和有限元分析得出，采用“V”型柔性铰链梁设计具有最优的轴向振动传递效率，同时对于径向残余振动的过滤效果也最佳。在中枢轴23的端面处设有螺纹孔，与压电陶瓷封装模块22的振动输出轴24外螺纹相配合。另外，为了加强中枢轴23和压电陶瓷封装模块振动输出轴24之间的强度，保证超声振动能的传递效率，在第二法兰26和中枢轴23之间设有若干第二加强筋27，位置为三个柔性铰链梁25中间，同样以轴线为中心呈120°阵列分布。在压电陶瓷封装模块22通过振动输出轴24将超声振动传递给柔性铰链机构20的中枢轴23的过程中，为避免两者在高频振动下螺纹连接发生松动，在柔性铰链机构20的中枢轴23圆柱面上设有螺纹通孔，当压电陶瓷封装模块22的振动输出轴24螺纹段完全拧入柔性铰链机构中枢轴23端面螺纹孔后，通过拧入紧定螺钉28对中枢轴23和振动输出轴24之间的连接起到加固和防松作用。
参图3、图12、图13所示，封盖10为二阶阶梯轴零件，在封盖10小直径段处设有外螺纹(未图示)，且封盖10大直径端面处设有光面通孔。柔性铰链机构20壳体端部的内壁末端相应位置设有一段内螺纹，恰与封盖10小轴端处外螺纹相配合。固定杆70同样为一个二阶阶梯轴零件，其端面小轴径段圆柱面设有外螺纹。
柔性铰链机构20、压电陶瓷封装模块22、封盖10、固定杆70、紧定螺钉28的装配过程：首先将压电陶瓷封装模块22的振动输出轴24部分拧入柔性铰链机构20中枢轴23端面处的螺纹孔中，随后将紧定螺钉28拧入中枢轴23圆柱面上的安装孔231，使得紧定螺钉28头部抵住振动输出轴24外螺纹 面上，起到放松加固作用。之后将封盖10的小轴端外螺纹与柔性铰链机构20壳体端面处内螺纹相配合。其次，将固定杆70小轴端插入封盖10大轴端面的光面通孔中，并将固定杆70小轴端的外螺纹拧入压电陶瓷封装模块22尾部端面处的内螺纹中。最后，通过不断拧紧固定杆70，使得压电陶瓷封装模块22尾部端面和封盖10小径段端面不断贴合拧紧，同时也带动压电陶瓷封装模块22跟转，使得压电陶瓷封装模块22振动输出轴24跟柔性铰链机构20中枢轴23螺纹孔不断拧紧固定，以及固定杆70阶梯轴端面和封盖10大轴径段端面相紧固贴合，从而实现压电陶瓷封装模块22、固定杆70、封盖10、柔性铰链机构壳体21四者的装配紧固过程。
本发明中基于柔性铰链机构的压电超声显微注射装置的工作原理为：
固定杆70和机架相固定，相对静地面保持绝对静止。固定杆70头部端面螺纹穿过封盖10通孔孔旋入压电陶瓷封装模块22尾部端面螺纹孔，通过旋紧使得封盖10和压电陶瓷封装模块22以及固定杆70三者相固定。且柔性铰链机构壳体21同样通过螺纹连接和封盖10相固定，因此柔性铰链机构壳体21、封盖10、固定杆70、压电陶瓷封装模块22主体部分均为固定端。压电陶瓷封装模块22接入电源后，振动输出轴24发生高频振动，通过振动输出轴24和中枢轴23之间的螺纹连接将振动传递给柔性铰链机构20的中枢轴23。由于压电陶瓷封装模块22在实际运动过程中会产生少量无规则径向振动。若直接将能量传递给基座30而不经过任何过滤缓冲，该径向有害振动在经过长远距离运输到达微针60针尖后，其径向残余振动会被放大成针尖的大幅度横向振动，不利于细胞显微注射和破膜。中枢轴在V型柔性铰链梁中，由于三方向柔性铰链的约束作用，使得中枢轴只能沿着轴线方向发生位移，而任意径向的残余振动会被三方向柔性铰链梁缓冲吸收。因此，柔性铰链机构的中枢轴、加强筋、法兰、输出轴为一整体，均为活动端，该整体相对于固定端段(静止参考)做直线高频往复运动。中枢轴最后将过滤后的超声振动能 传递给振动输出轴，并最后通过振动输出轴和基座的螺纹连接将振动能传递给基座。
由以上技术方案可以看出，本发明具有如下有益效果：
针对压电陶瓷封装模块传导到微针针尖的有害径向振动，对压电陶瓷封装模块设计了一种三维立体柔性铰链机构，在保持高能量传输效率的同时，也能有效过滤、缓冲振动输出轴的径向有害振动，从而减少微针针尖的横向有害振动；
双锥面异形垫圈自密封机构有效的实现了气密封和液密封的功能，同时该机构对微针具有更好的夹持稳固作用；
对“柔性铰链机构-基座”超声能量传递连接部分进行优化，即采用法兰盘面接触和加强筋的设计，在不削弱微针整体强度和功能的前提下，大大减轻了机构整体质量，提高了压电陶瓷的能量传递效率。
因此，本发明可以满足不同需求的生物工程显微操作实验，如：细胞核移植、细胞单精子注射、转基因注射等。同时，由于微针夹持段采用了加强筋设计，极大的减轻了显微注射器的整体质量，并配合三维立体柔性铰链机构，有效减轻由压电陶瓷造成的微针针尖径向振动，从而有效缓解注射针对细胞造成的机械损伤，提高实验成功率。同时，本发明结构简单、各零部件互换性好，易于装配，且加工成本相对较低，简化了显微注射实验操作的实验成本和复杂程度。
对于本领域技术人员而言，显然本发明不限于上述示范性实施例的细节，而且在不背离本发明的精神或基本特征的情况下，能够以其他的具体形式实现本发明。因此，无论从哪一点来看，均应将实施例看作是示范性的，而且是非限制性的，本发明的范围由所附权利要求而不是上述说明限定，因此旨在将落在权利要求的等同要件的含义和范围内的所有变化囊括在本发明内。不应将权利要求中的任何附图标记视为限制所涉及的权利要求。
此外，应当理解，虽然本说明书按照实施方式加以描述，但并非每个实施方式仅包含一个独立的技术方案，说明书的这种叙述方式仅仅是为清楚起见，本领域技术人员应当将说明书作为一个整体，各实施例中的技术方案也可以经适当组合，形成本领域技术人员可以理解的其他实施方式。

Claims (16)

  1. 一种基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述装置包括：
    固定装配的封盖、柔性铰链机构、基座、旋盖及端盖，所述基座上设有泵接口；
    固定安装于基座、旋盖及端盖内且向外延伸的微针，所述微针与泵接口相连通；
    所述柔性铰链机构包括壳体、封装于壳体内的压电陶瓷封装模块、与压电陶瓷封装模块及基座固定安装的中枢轴、自压电陶瓷封装模块延伸至中枢轴内的振动输出轴，所述中枢轴与壳体之间设有若干柔性铰链梁。
  2. 根据权利要求1所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述端盖与所述旋盖、所述旋盖与所述基座之间分别通过螺纹固定安装。
  3. 根据权利要求2所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述端盖与旋盖之间和/或旋盖与基座之间设有垫圈，所述垫圈的两侧为不同的第一锥面和第二锥面。
  4. 根据权利要求2所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述端盖包括端盖主体及端盖安装部，所述端盖主体的外径大于端盖安装部的外径，所述端盖安装部的外侧设有与旋盖固定安装的第一螺纹。
  5. 根据权利要求4所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述端盖内自端盖主体向端盖安装部方向形成有第一腔体和第二腔体，所述第二腔体呈锥形。
  6. 根据权利要求3所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述旋盖包括旋盖主体及旋盖安装部，所述旋盖主体的外径大于旋盖安装部的外径，所述旋盖主体的内侧设有与端盖固定安装的第二螺纹， 所述旋盖安装部的外侧设有与基座固定安装的第三螺纹。
  7. 根据权利要求6所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述旋盖内自旋盖主体向旋盖安装部方向形成有第三腔体、第四腔体和第五腔体，所述第五腔体呈锥形。
  8. 根据权利要求2所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述基座包括与旋盖固定安装的折弯段、以及与柔性铰链机构固定安装的水平段，所述折弯段及水平段通过螺纹与旋盖及柔性铰链机构分别固定安装。
  9. 根据权利要求8所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述折弯段的内侧设有与旋盖固定安装的第四螺纹。
  10. 根据权利要求8所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述折弯段内部设有第六腔体及第七腔体，水平段内部设有第八腔体，所述第七腔体呈锥形，所述第六腔体通过第七腔体及微流道与泵接口相连通。
  11. 根据权利要求8所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述水平段的内侧设有第五螺纹，柔性铰链机构的中枢轴端部外侧设有第六螺纹，所述基座与柔性铰链机构通过第五螺纹和第六螺纹固定安装。
  12. 根据权利要求8所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述水平段的端部设有第一法兰，第一法兰和水平段之间设有若干第一加强筋。
  13. 根据权利要求12所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述柔性铰链机构的中枢轴上设有第二法兰，所述第二法兰与中枢轴之间设有若干第二加强筋。
  14. 根据权利要求1所述的基于柔性铰链机构的压电超声显微注射装置， 其特征在于，所述柔性铰链梁上设有若干V型凹陷部。
  15. 根据权利要求1所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述柔性铰链梁以中枢轴轴心为中心等角度120°圆周阵列分部，且每个方向的柔性铰链梁均在轴向方向相隔一定距离呈等距双柔性铰链梁平行线性阵列分布。
  16. 根据权利要求1所述的基于柔性铰链机构的压电超声显微注射装置，其特征在于，所述中枢轴上设有安装孔，安装孔内固定安装有螺钉，以固定所述中枢轴和振动输出轴。
PCT/CN2018/087759 2018-05-14 2018-05-22 基于柔性铰链机构的压电超声显微注射装置 WO2019218379A1 (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/958,729 US11312934B2 (en) 2018-05-14 2018-05-22 Piezoelectric ultrasonic microinjection device based on flexible hinge mechanism

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201810454388.0 2018-05-14
CN201810454388.0A CN108330061B (zh) 2018-05-14 2018-05-14 基于柔性铰链机构的压电超声显微注射装置

Publications (1)

Publication Number Publication Date
WO2019218379A1 true WO2019218379A1 (zh) 2019-11-21

Family

ID=62935073

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2018/087759 WO2019218379A1 (zh) 2018-05-14 2018-05-22 基于柔性铰链机构的压电超声显微注射装置

Country Status (3)

Country Link
US (1) US11312934B2 (zh)
CN (1) CN108330061B (zh)
WO (1) WO2019218379A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111889680A (zh) * 2020-06-09 2020-11-06 哈尔滨工业大学 一种基于压电微喷的热熔金属微液滴直接按需打印机构

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109207343A (zh) * 2018-08-24 2019-01-15 苏州大学 一种基于三角形柔性机构的细胞显微注射装置
CN109604814B (zh) * 2018-12-10 2021-02-26 东北大学 一种透镜轴向低频振动辅助激光加工装置
CN109988702A (zh) * 2019-05-10 2019-07-09 苏州大学 一种模块化设计的适配多型针头的压电超声显微注射装置
CN111514451B (zh) * 2020-03-30 2022-04-05 华东师范大学 一种基于超声雾化的微针美容装置
CN111855032A (zh) * 2020-07-06 2020-10-30 南京航空航天大学 一种基于柔性铰链对称结构的压电触觉传感器
CN114107023B (zh) * 2021-11-23 2024-03-19 南京航空航天大学 一种压电驱动细胞显微注射装置及其自适应柔顺控制方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008046051A2 (en) * 2006-10-12 2008-04-17 University Of Connecticut Rotationally oscillating injector
US20090130743A1 (en) * 2005-06-30 2009-05-21 Fujio Miyawaki Vibration type microinjection device
EP2017330B1 (en) * 2007-07-18 2010-08-11 Fujitsu Limited Microinjection apparatus and method of injecting fluid
CN102268363A (zh) * 2011-06-22 2011-12-07 苏州大学 显微注射机构
CN102268360A (zh) * 2011-04-27 2011-12-07 南开大学 基于压电陶瓷驱动的超声振动显微细胞穿入系统
CN104109629A (zh) * 2014-07-30 2014-10-22 苏州大学 一种压电超声显微注射器及压电超声显微注射系统

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8123594A (en) * 1993-10-22 1995-05-08 Abbott Laboratories Reaction tube and method of use to minimize contamination
CN2589778Y (zh) * 2001-04-17 2003-12-03 陈军 自锁连接件
RU2211395C1 (ru) * 2001-12-05 2003-08-27 Открытое акционерное общество "Ракетно-космическая корпорация "Энергия" им. С.П. Королева" Разъемное соединение трубопроводов
CN102497129B (zh) * 2011-12-06 2014-06-04 浙江大学 由多极化方式压电驱动器驱动的多自由度微操作器
CN203054234U (zh) * 2012-11-29 2013-07-10 中国矿业大学 一种矿山震动信号接收传感装置
CN202972264U (zh) * 2012-12-18 2013-06-05 郑州人造金刚石及制品工程技术研究中心有限公司 一种组合式防松动管接头密封结构
CN103950027A (zh) * 2014-04-24 2014-07-30 南京航空航天大学 基于直线型超声电机的二指并联微操作手及操作方法
CN105042018A (zh) * 2015-06-30 2015-11-11 南平市建阳区汽车锻压件厂 设有减振结构的液压驱动摩擦锁止直连式差速器
DE102018103049A1 (de) * 2018-02-12 2019-08-14 Karlsruher Institut für Technologie Druckkopf und Druckverfahren
CN208430152U (zh) * 2018-05-14 2019-01-25 苏州大学张家港工业技术研究院 基于柔性铰链机构的压电超声显微注射装置

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090130743A1 (en) * 2005-06-30 2009-05-21 Fujio Miyawaki Vibration type microinjection device
WO2008046051A2 (en) * 2006-10-12 2008-04-17 University Of Connecticut Rotationally oscillating injector
EP2017330B1 (en) * 2007-07-18 2010-08-11 Fujitsu Limited Microinjection apparatus and method of injecting fluid
CN102268360A (zh) * 2011-04-27 2011-12-07 南开大学 基于压电陶瓷驱动的超声振动显微细胞穿入系统
CN102268363A (zh) * 2011-06-22 2011-12-07 苏州大学 显微注射机构
CN104109629A (zh) * 2014-07-30 2014-10-22 苏州大学 一种压电超声显微注射器及压电超声显微注射系统

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111889680A (zh) * 2020-06-09 2020-11-06 哈尔滨工业大学 一种基于压电微喷的热熔金属微液滴直接按需打印机构
CN111889680B (zh) * 2020-06-09 2022-08-26 哈尔滨工业大学 一种基于压电微喷的热熔金属微液滴直接按需打印机构

Also Published As

Publication number Publication date
CN108330061A (zh) 2018-07-27
US11312934B2 (en) 2022-04-26
CN108330061B (zh) 2024-02-09
US20200332244A1 (en) 2020-10-22

Similar Documents

Publication Publication Date Title
WO2019218379A1 (zh) 基于柔性铰链机构的压电超声显微注射装置
CN208430152U (zh) 基于柔性铰链机构的压电超声显微注射装置
JP2016148404A (ja) ゲートバルブ
CN208587343U (zh) 一种溢流阀
CN215527858U (zh) 一种挂载组件及电池包
CN113583847A (zh) 一种细胞显微注射装置及其鲁棒阻抗控制方法
CN108893248A (zh) 压电超声显微注射器
CN111022654A (zh) 一种低温阀
CN107028643A (zh) 一种超声换能装置
JP6810838B1 (ja) 弾性係数可変のばね構成材
CN213749521U (zh) 一种超声波岩心夹持器
CN201377628Y (zh) 弹性阻尼体减震缓冲阻尼器介质注入用单向阀
CN203976782U (zh) 一种压电超声显微注射器及压电超声显微注射系统
CN108443605A (zh) 高压气瓶气密试验接头
CN210692254U (zh) 一种电磁铁气隙限位调整装置结构
CN205056416U (zh) 一种超声换能器
CN216767593U (zh) 一种火箭发动机用增压阀结构
CN212888859U (zh) 一种挤出机的脱挥装置
CN218236147U (zh) 一种端面浮动静密封结构
CN216077780U (zh) 一种工程机械液压阀阀芯结构
CN210637204U (zh) 一种发动机进气稳压装置
CN217951636U (zh) 一种燃气管封堵器
CN209625807U (zh) 一种卡接柱腔
CN220227155U (zh) 一种密封效果好的液压泵
CN218780779U (zh) 一种高密封性蝶阀

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18919300

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18919300

Country of ref document: EP

Kind code of ref document: A1