WO2019205943A1 - 一种改善胰岛素抵抗的复合制剂及制备方法与应用 - Google Patents
一种改善胰岛素抵抗的复合制剂及制备方法与应用 Download PDFInfo
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- WO2019205943A1 WO2019205943A1 PCT/CN2019/082127 CN2019082127W WO2019205943A1 WO 2019205943 A1 WO2019205943 A1 WO 2019205943A1 CN 2019082127 W CN2019082127 W CN 2019082127W WO 2019205943 A1 WO2019205943 A1 WO 2019205943A1
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- extract
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- improving insulin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/733—Fructosans, e.g. inulin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
- A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
Definitions
- the present disclosure relates to the field of oral preparations, and in particular to a composite preparation for improving insulin resistance, a preparation method and application thereof.
- the object of the present disclosure includes, for example, providing a composite preparation for improving insulin resistance which is safe and effective in improving insulin resistance.
- the object of the present disclosure also includes, for example, a method for preparing a composite preparation for improving insulin resistance, which is simple in process and easy to industrially produce.
- the object of the present disclosure also includes, for example, the use of a composite preparation for improving insulin resistance for the preparation of an oral preparation for improving insulin resistance.
- the present disclosure provides a composite preparation for improving insulin resistance, which is prepared from the following raw materials, and the raw materials include:
- the oligosaccharide is selected from the group consisting of inulin, oligofructose, xylooligosaccharide, galactooligosaccharide, oligo-isomaltose, polydextrose, resistant dextrin, L-arabinose, and konjac At least one of a combination of glucomannan compositions.
- the plant extract is selected from the group consisting of seaweed extract, kelp extract, yellow essence extract, alfalfa extract, alfalfa extract, puerarin and ginseng extract, and psyllium husk At least one of the combinations.
- the fungal extract is selected from at least one of the group consisting of a mushroom extract, a cordyceps extract, a ganoderma lucidum extract, a Hericium erinaceus extract, and a yeast extract.
- the raw material further comprises Tremella extract, and the Tremella extract is used in an amount of from 1% to 5% by mass of the fungal extract.
- the feedstock further comprises Dendrobium candidum extract, the Dendrobium candidum extract being from 1% to 5% by mass of the plant extract.
- the present disclosure provides a method for preparing the above-described composite preparation for improving insulin resistance, which comprises the following steps:
- the oligosaccharide, the plant extract, the fungal extract, the sodium taurine and the Cistanche extract are mixed and ground.
- the plant extract is prepared as follows:
- the selected plant raw materials are washed, pulverized and mixed, and added with 4-6 times weight and 60 ° C-80 ° C hot water extraction for 3-4 hours, and filtered to obtain an extract; after the extract is concentrated, ethanol precipitation is carried out to obtain a crude polysaccharide.
- the crude polysaccharide is separated by chromatography and eluted with distilled water to obtain a polysaccharide liquid; after the polysaccharide liquid is concentrated, it is precipitated with ethanol and dried to obtain a powdery plant extract.
- the fungal extract is prepared as follows:
- the fungal raw material is dried and fully ground, and then added with 10-20 volumes of pure water, extracted at 70-80 ° C for 3-4 hours, and filtered to obtain an extract; the extract is centrifuged at 3000-5000 r/min for 10-15 minutes. The supernatant was taken; the supernatant was concentrated, and precipitated with ethanol; the precipitate was taken and dried to obtain a powdery fungal extract.
- the present disclosure provides the use of the above-described composite preparation for improving insulin resistance, which is used for preparing an oral preparation for reducing the ratio of the thick-walled mycelium/Bacteroides in the intestinal tract and increasing the specific gravity of the SCFA-producing bacteria.
- Embodiments of the present disclosure provide the use of a composite preparation for improving insulin resistance according to the present disclosure for improving insulin resistance or treating or preventing a disease or condition associated with insulin resistance.
- Embodiments of the present disclosure provide a method of treating or preventing a disease or condition associated with insulin resistance or insulin resistance, comprising administering to a subject in need thereof a composite preparation for improving insulin resistance as described in the present disclosure, or an improvement as described in the present disclosure A combination of insulin resistance and a pharmaceutically acceptable carrier.
- the insulin resistance-related disease or condition is metabolic syndrome, obesity, type 2 diabetes, hyperinsulinemia, polycystic ovary syndrome, hyperglycemia, hypertension, or dyslipidemia .
- the composite preparation that improves insulin resistance is an oral powder formulation.
- the beneficial effects of the composite preparation for improving insulin resistance and the preparation method and application thereof of the embodiments of the present disclosure include at least: a composite preparation for improving insulin resistance according to an embodiment of the present disclosure, which is prepared from the following raw materials in mass percentage: 20% -65% oligosaccharides; 21%-44% plant extract; 10%-30% fungal extract; 0.5%-5% sodium taurate; and 0.5%-5% Cistanche extract, the safety of the composite preparation Good sex, can effectively regulate the intestinal flora.
- the preparation method of the composite preparation is that the oligosaccharide, the plant extract, the fungal extract, the sodium taurine and the Cistanche extract are mixed and ground, and the process is simple and easy to industrialize.
- the use of the composite preparation for improving insulin resistance of the embodiments of the present disclosure is a composite preparation for preparing an oral preparation for improving insulin resistance.
- the embodiments of the present disclosure provide a composite preparation for improving insulin resistance, which is prepared from the following raw materials, and the raw materials include: 20%-65% oligosaccharides; 21%-44% plant extracts; %-30% fungal extract; 0.5%-5% sodium taurate; and 0.5%-5% Cistanche extract.
- the raw material comprises: 33% - 52% oligosaccharide; 29% - 36% plant extract; 15% - 25% fungal extract; 2% - 5% sodium taurate; and 1% - 4% Cistanche Extract.
- the oligosaccharide is selected from the group consisting of inulin, oligofructose, xylooligosaccharide, galactooligosaccharide, oligo-isomaltose, polydextrose, resistant dextrin, L-arabinose, and konjac At least one of a combination of glucomannan compositions.
- the raw materials include 1%-10% inulin, 1%-5% xylooligosaccharide, 1.5%-10% galacto-oligosaccharide, 1%-5% oligo-isomaltose, 5%-by mass percent. 10% polydextrose, 5%-10% resistant dextrin, 5%-10% L arabinose and 0.5%-5% konjac glucomannan.
- the plant extract is obtained by plant extraction, mostly plant polysaccharides.
- the plant extract is selected from the group consisting of seaweed extract, kelp extract, extract of Rhizoma Polygonati, extract of Lycium barbarum, extract of Lycium barbarum L., extract of Radix Puerariae (ie, Pueraria lobata extract), and ginseng extract, and composition of psyllium psyllium. At least one of the combinations.
- the front hull of the round shovel is the outer shell of the seed of the planted psyllium (Llant name: Plantago ovata), which can be directly ground.
- the raw materials include 5%-10% seaweed extract, 3%-5% kelp extract, 1.5%-5% yellow essence extract, 3%-5% quince extract, 5%-10% by mass percentage.
- the fungal extract is obtained by fungal extraction, mostly fungal polysaccharides.
- the fungal extract is at least one selected from the group consisting of a mushroom extract, a cordyceps extract, a ganoderma lucidum extract, a Hericium erinaceus extract, and a yeast extract.
- the raw materials include 5%-10% shiitake mushroom extract, 0.5%-5% Cordyceps extract, 0.5%-5% Hericium erinaceus extract, and 4%-10% yeast extract by mass percentage.
- the raw material may further comprise Tremella extract, and the Tremella extract is used in an amount of from 1% to 5% by mass of the fungal extract.
- the feedstock further comprises Dendrobium candidum extract, the Dendrobium candidum extract being from 1% to 5% by mass of the plant extract.
- the raw material further comprises a flower extract
- the flower extract is used in an amount of from 2% to 10% by mass of the plant extract.
- the flower extract is selected from at least one of a combination of a peony flower extract, a honeysuckle extract, and a dandelion extract.
- the composite formulation is made using oligosaccharides, plant extracts, fungal extracts, sodium taurate, and Cistanche extract, wherein oligosaccharides, plant extracts, fungal extracts, cattle Sodium sulfonate synergistically improves insulin resistance.
- Embodiments of the present disclosure provide a method of preparing the above-described composite preparation for improving insulin resistance, comprising the steps of:
- the oligosaccharide, the plant extract, the fungal extract, the sodium taurine and the Cistanche extract are mixed and ground.
- the plant extract can be purchased as a high purity extract, or can be prepared according to the following preparation methods:
- the selected plant raw materials are washed, pulverized and mixed, and added with 4-6 times weight and 60 ° C-80 ° C hot water extraction for 3-4 hours, and filtered to obtain an extract; after the extract is concentrated, ethanol precipitation is carried out to obtain a crude polysaccharide.
- the crude polysaccharide is separated by chromatography and eluted with distilled water to obtain a polysaccharide liquid; after the polysaccharide liquid is concentrated, it is precipitated with ethanol and dried to obtain a powdery plant extract.
- the fungal extract can be purchased as a high purity extract, or can be prepared according to the following preparation methods:
- the fungal raw material is dried and fully ground, and then added with 10-20 volumes of pure water, extracted at 70-80 ° C for 3-4 hours, and filtered to obtain an extract; the extract is centrifuged at 3000-5000 r/min for 10-15 minutes. The supernatant was taken; the supernatant was concentrated, and precipitated with ethanol; the precipitate was taken and dried to obtain a powdery fungal extract.
- the flower extract is commercially available or can be prepared according to the following preparation methods:
- the flower raw material is added to the yeast for fermentation, filtered, matured, and clarified to obtain a flower and fruit fermentation liquid; the fermentation liquid is filtered and concentrated to obtain a powdery flower extract.
- the Cistanche extract is commercially available or can be prepared according to the following preparation methods:
- Cistanche deserticola microwave extraction device microwave power 500W-900W, temperature 70 °C, a total of 3 extractions, the first addition of 50%-90% ethanol, extraction 10min-30min; the second addition 50%-90% Ethanol, extraction for 10min-30min; the third addition of distilled water, extraction 20min-40min.
- the first and second ethanol extracts were concentrated to remove ethanol, and the third aqueous extract was added thereto, mixed, and concentrated to obtain a powdery Cistanche extract.
- Embodiments of the present disclosure provide an application of the above-described composite preparation for improving insulin resistance.
- the composite preparation has a function of improving insulin resistance, thereby increasing sensitivity to insulin. Accordingly, the composite preparation can be used to prepare an oral preparation for improving insulin resistance. More specifically, the composite preparation is used for the preparation of an oral preparation for improving the symptoms of obesity in obese people.
- oligosaccharide 100 g inulin, 100 g oligofructose, 100 g xylooligosaccharide, 90 g galactooligosaccharide, 50 g oligo-isomaltose, 50 g polydextrose, 50 g resistant dextrin, 50 g L arabinose and 40 g Konjac glucomannan
- 230 g plant extract 50 g kelp extract, 80 g scutellaria extract, 50 g sorghum extract, 50 g sorghum extract
- 120 g fungal extract 60 g mushroom extract, 40 g cordyceps extract, 20 g yeast) Extract
- 10 g of sodium taurate 60 g mushroom extract, 40 g cordyceps extract, 20 g yeast
- 220 g oligosaccharide 50 g galactooligosaccharide, 50 g oligo isomaltose, 50 g polydextrose, 50 g L arabinose and 20 g konjac glucomannan
- 430 g plant extract 100 g kelp extract, 80 g huangjing extract
- 300 g of fungal extract 100 g of mushroom extract, 50 g of cordyceps extract, 100 g of extract of Hericium erinaceus and 50 g of yeast extract
- 25 g Sodium taurate and 25 g of Cistanche extract.
- oligosaccharide 100 g inulin, 100 g oligofructose, 100 g xylooligosaccharide, 100 g galactooligosaccharide, 100 g oligo-isomalt
- 300 g plant extract 100 g seaweed extract, 100 g yellow essence extract
- 50g of alfalfa extract, 50g of alfalfa extract 50g of alfalfa extract
- 150g of fungal extract 50g of mushroom extract, 50g of cordyceps extract, 50g of extract of Hericium erinaceus
- 25g of sodium taurate 50g of Dendrobium Extract.
- the oligosaccharide, the plant extract, the fungal extract, the sodium taurine and the Cistanche extract, the Dendrobium candidum extract are mixed and ground.
- oligosaccharide 50g inulin, 50g oligofructose, 50g xylooligosaccharide, 50g galactooligosaccharide, 50g oligo-isomaltose, 50g polydextrose, 50g resistant dextrin
- 350g plant extract 50 g seaweed extract, 50 g kelp extract, 100 g scutellaria extract, 50 g sorghum extract, 50 g sorghum extract and 50 g ginseng extract
- 250 g fungal extract 100 g mushroom extract, 50 g cordyceps extract, 50 g monkey head
- Mushroom extract and 50 g yeast extract 25 g sodium taurine; and 25 g Cistanche extract
- 8 g Tremella extract 50g inulin, 50g oligofructose, 50g xylooligosaccharide, 50g galactooligosaccharide, 50g oligo-isomaltose,
- the oligosaccharide, the plant extract, the fungal extract, the sodium taurine and the Cistanche extract, the white fungus extract are mixed and ground.
- oligosaccharide 50g inulin, 50g xylooligosaccharide, 50g galacto-oligosaccharide, 30g oligo-isomaltose, 50g polydextrose, 50g resistant dextrin, 100gL arabinose and 40g konjac glucomannan
- 320g plant extract 50g seaweed extract, 50g kelp extract, 50g scutellaria extract, 30g sorghum extract, 60g sorghum extract and 30g puerarin, 50g psyllium husk
- 200g fungal extract 40 g of shiitake mushroom extract, 40 g of Cordyceps extract, 40 g of extract of Hericium erinaceus and 40 g of yeast extract
- 20 g of sodium taurate and 40 g of Cistanche extract.
- oligosaccharide 50g inulin, 50g xylooligosaccharide, 50g galacto-oligosaccharide, 30g oligo-isomaltose, 50g polydextrose, 50g resistant dextrin, 100gL arabinose and 40g konjac glucomannan
- 320g plant extract 50g seaweed extract, 50g kelp extract, 50g scutellaria extract, 30g sorghum extract, 60g sorghum extract and 30g puerarin, 50g psyllium husk
- 200g fungal extract 40 g of mushroom extract, 40 g of Cordyceps extract, 40 g of extract of Hericium erinaceus and 40 g of yeast extract
- 20 g of sodium taurate and 40 g of Cistanche extract
- 30 g of peony extract 50g inulin, 50g xylooligosaccharide, 50g galacto-oligosaccharide, 30g
- mice Female BALb/c mice, 4 weeks old (body weight 20 ⁇ 1g), SPF grade, were purchased from Hubei Experimental Animal Research Center. Raised in the SPF animal room. Oral feed (fat calorie 10%) and high-fat high-protein feed (fat calories 40% or more) fed to mice were purchased from the Hubei Animal Research Center, and mice were given free access to water during feeding.
- mice After adaptive feeding for 1 week, the mice were randomly divided into 3 groups, 12 in each group:
- Blank control group continuous feeding of common feed
- Conditional control group After feeding high-fat feed for 6 weeks, the feed was changed to normal feed for 1 week;
- Example 5 The compound preparation of Example 5 was changed to 6 weeks after continuous feeding of the high fat diet, and each mouse was perfused for 1 week at a dose of 1 g/kg.
- mice obesity model The results of induction of mouse obesity model can be seen from the table.
- the changes in body weight of the high-fat diet (P ⁇ 0.05) in the control group and the experimental group were significantly higher than those in the control group.
- the weight gain of the comparison group was ⁇ 20%.
- Mouse obesity model The changes in body weight of the high-fat diet (P ⁇ 0.05) in the control group and the experimental group were significantly higher than those in the control group.
- the weight gain of the comparison group was ⁇ 20%.
- mice were fasted for 4 h, and the blood glucose concentration of the tail vein of the mice (Roche blood glucose meter) was measured. All blood samples were tested for blood insulin levels according to the Rat/Mouse Insulin ELISA Kit (Millipore, US) kit operating instructions.
- mice were fasted for 12 h, and the body weight of the mice and the blood of the tail vein were measured to determine the blood glucose level of the fasting state of the mice;
- the glucose solution was intragastrically administered with a 50% glucose sugar solution at a dose of 2 g/kg; the blood glucose concentration of the tail vein of the mouse was measured at intervals of 30 min, 60 min, 90 min, and 120 min.
- mice were fasted for 12 h, and the mice were bled with blood. All blood samples were processed according to the kit instructions; cholesterol and free fatty acids and glycated serum protein kit (Wuhan Shengzhiyuan Biotechnology Co., Ltd.).
- the results of oral glucose tolerance test showed that the glucose tolerance of the control group was abnormal (P ⁇ 0.01). Compared with the glucose tolerance of the blank group, the glucose tolerance of the experimental group was significantly lower than that of the control group. P ⁇ 0.05). It is indicated that the composite preparation can effectively reduce insulin resistance caused by obesity.
- the data results involved in this test are all measurement data, and all data are in mean and standard deviation. Said.
- the composite preparation for improving insulin resistance of the embodiments of the present disclosure has good safety and can effectively improve insulin resistance;
- the preparation method of the composite preparation is simple in process and easy to industrialize.
- the use of the composite preparation for improving insulin resistance of the embodiments of the present disclosure is a composite preparation for preparing an oral preparation for improving insulin resistance.
- the composite preparation of the present disclosure has good safety and can effectively regulate intestinal flora.
- the preparation preparation of the composite preparation of the present disclosure is simple and easy to industrialize production.
- the use of the composite preparation for improving insulin resistance of the embodiments of the present disclosure is a composite preparation for preparing an oral preparation for improving insulin resistance.
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Abstract
Description
Claims (15)
- 根据权利要求1所述的改善胰岛素抵抗的复合制剂,其特征在于,所述低聚糖选自菊粉、低聚果糖、低聚木糖、低聚半乳糖、低聚异麦芽糖、聚葡萄糖、抗性糊精、L阿拉伯糖和魔芋葡甘聚糖组成的组合中的至少一种。
- 根据权利要求1或2所述的改善胰岛素抵抗的复合制剂,其特征在于,所述植物提取物选自海藻提取物、昆布提取物、黄精提取物、茯苓提取物、枸杞提取物、葛根黄酮和人参提取物、圆苞车前子壳组成的组合中的至少一种。
- 根据权利要求1至3中任一项所述的改善胰岛素抵抗的复合制剂,其特征在于,所述真菌提取物选自香菇提取物、虫草提取物、灵芝提取物、猴头菇提取物和酵母提取物组成的组合中的至少一种。
- 根据权利要求1至4中任一项所述的改善胰岛素抵抗的复合制剂,其特征在于,所述原料还包括银耳提取物,所述银耳提取物的使用量为所述真菌提取物质量的1%-5%。
- 根据权利要求1至5中任一项所述的改善胰岛素抵抗的复合制剂,其特征在于,所述原料还包括铁皮石斛提取物,所述铁皮石斛提取物为所述植物提取物质量的1%-5%。
- 根据权利要求1至4中任一项所述的改善胰岛素抵抗的复合制剂,其特征在于,所述原料包括630g低聚糖;230g植物提取物;120g真菌提取物;10g牛磺酸钠;以及10g肉苁蓉提取物,其中,所述低聚糖由100g菊粉、100g低聚果糖、100g低聚木糖、90g低聚半乳糖、50g低聚异麦芽糖、50g聚葡萄糖、50g抗性糊精、50gL阿拉伯糖和40g魔芋葡甘聚糖组成,所述植物提取物由50g昆布提取物、80g黄精提取物、50g茯苓提取物和50g枸杞提取物组成,以及所述真菌提取物由60g香菇提取物、40g虫草提取物和20g酵母提取物组成。
- 一种如权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂的制备方法,其特征在于,其包括以下步骤:将低聚糖、植物提取物、真菌提取物、牛磺酸钠和肉苁蓉提取物混合、研磨即得。
- 根据权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂或根据权利要求8所述的方法,其特征在于,所述植物提取物的制备方法如下:将选取的植物原料清洗、粉碎后混合,添加4-6倍重量、60℃-80℃的热水提取3-4小时,过滤得到提取液;将提取液浓缩后,再用乙醇沉淀得到粗品多糖;将粗品多糖经层析分离、蒸馏水洗脱,得到多糖液;将多糖液浓缩后,再用乙醇沉淀,干燥得到粉状的植物提取物。
- 根据权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂或根据权利要求8所述的方法,其特征在于,所述真菌提取物的制备方法如下:将真菌原料干燥后充分研磨,再加10-20倍体积纯水,于70-80℃下提取3-4小时,过滤得到提取液;将提取液经3000-5000r/min离心10-15分钟后取上清液;将上清液浓缩后,用乙醇沉淀;取沉淀,干燥后得到粉状的真菌提取物。
- 一种如权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂的应用,其特征在于,所述复合制剂用于制备改善胰岛素抵抗的口服制剂。
- 根据权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂用于改善胰岛素抵抗或治疗或预防胰岛素抵抗相关疾病或疾患的应用。
- 一种治疗或预防胰岛素抵抗或胰岛素抵抗相关疾病或疾患的方法,包括向有需要的受试者给予如权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂,或如权利要求1至7中任一项所述的改善胰岛素抵抗的复合制剂以及药学上可接受的载体。
- 根据权利要求12所述的应用或权利要求13所述的方法,其中所述胰岛素抵抗相关疾病或疾患为代谢综合征、肥胖症、2型糖尿病、高胰岛素血症、多囊卵巢综合症、高血糖症、高血压或血脂紊乱。
- 根据权利要求11或12所述的应用,或权利要求13所述的方法,所述改善胰岛素抵抗的复合制剂为口服粉末制剂。
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