WO2019158786A1 - FORMULADO LÍQUIDO A BASE DE NANOPARTÍCULAS DE CuO COMO POTENCIADOR DE LA AUTODEFENSA DE LAS PLANTAS Y USO DEL MISMO - Google Patents
FORMULADO LÍQUIDO A BASE DE NANOPARTÍCULAS DE CuO COMO POTENCIADOR DE LA AUTODEFENSA DE LAS PLANTAS Y USO DEL MISMO Download PDFInfo
- Publication number
- WO2019158786A1 WO2019158786A1 PCT/ES2018/070181 ES2018070181W WO2019158786A1 WO 2019158786 A1 WO2019158786 A1 WO 2019158786A1 ES 2018070181 W ES2018070181 W ES 2018070181W WO 2019158786 A1 WO2019158786 A1 WO 2019158786A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plant
- plants
- copper
- formulation
- cuo nanoparticles
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Definitions
- the present invention relates to a liquid formulation based on copper oxide nanoparticles (CuO) as a plant self-defense enhancer.
- CuO copper oxide nanoparticles
- the invention also relates to the use of said formulation in the defense of plants and trees such as vines, almond trees and others of a similar nature against certain pathogens that cause fungal diseases in the wood of such plants.
- the technical field in which the present invention is registered is included within the industrial sector dedicated to the manufacture and application of fertilizers and other products to promote and enhance the self-defense of trees and plants.
- EFMV Fungal wood diseases
- REPLACEMENT SHEET (RULE 26) this formulated to the vines has allowed to improve the metabolic processes that occur during the different stages of development, improving the appearance of the treated plants, activating their self-defense and resistance to biotic and abiotic adverse conditions, thereby allowing to increase e! yield and crop production.
- wood diseases are multiannual and are caused by more of 100 different fungal species, associated with a set of symptoms.
- Pathogenic fungi infect the wood and develop internally in the trunk and arms, causing a necrosis and / or wood rot.
- the diseased vines have a progressive loss of vigor, reducing the quantity and quality of the harvest year after year.
- a progressive decay occurs and the plants end up dying, which forces to replace the vineyard with the consequent economic consequences that all this entails.
- copper has the characteristic of activating certain enzymes involved in the synthesis of lignin in plants and is essential for various enzyme systems. It is also necessary in the process of photosynthesis, essential for the respiration of plants and adjuvant of these in the metabolism of carbohydrates and proteins, also helping to intensify the taste and color in vegetables and flowers.
- REPLACEMENT SHEET (RULE 26) It is therefore clear that copper is a necessary element for plants. However, it often happens that the contribution of this element to plants occurs in amounts that exceed the allowed limits, generating a toxicity that affects the development of the root, burning its tips and causing excessive lateral growth. It must also be taken into account that high levels of copper in plants can compete with the absorption of other elements, such as iron, molybdenum or zinc. In the case of new crops, at first they may become greener than normal, then they will show symptoms of iron deficiency and probably other micronutrients, and if not corrected in time, the threat of copper toxicity can reduce branching. and finally cause the deterioration of the plant.
- the present invention relates to a liquid formulation based on CuO nanoparticles, in the form of copper oxide nanoparticles (CuO), as a plant self-defense enhancer and intended to improve their physiological efficiency. More particularly, the invention relates to a liquid formulation that makes it possible to reduce the incidence and severity of the symptoms of diseases in plants, in particular of wood (inhibiting the action of certain pathogens) and fungal diseases such as Mildiu, while reducing perspiration and achieving greater net photosynthesis. Similarly, one of the fundamental characteristics of the present formulation is the low concentration of copper that it presents, which makes it a formula that is quite environmentally friendly.
- the use of the formulated invention allows to improve the metabolic processes that occur during the different stages of development, improving the appearance of the treated plants, activating their self-defense and resistance to biotic and abiotic adverse conditions, thereby increasing the yield and crop production.
- the formulation of the present invention includes copper, essential plant amino acids and a derivative of vitamin K (MSB, add sodium bisulfite or Vitamin K3), with the particularity that copper is incorporated in the form of nanoparticles, being able to multiply in this way, the protective surface of the treatments while ensuring better adhesion and persistence of copper on the plant tissues, thus increasing the crop protection time.
- This composition also allows the formulation of the invention to also act as a stimulant of certain metabolic reactions of the basic plants for their development and growth.
- nanotechnology can offer nanomaterials that can act as an elici ⁇ or for pfantas, in addition to other functions.
- An elicitor is defined as a compound that, when applied exogenously, is capable of activating or triggering the natural synthesis of other substances in the plant, stimulating the metabolism and activating the defensive response of the plant. But not only that, nanomaterials can act as more effective nutrients or pesticides with fewer side effects and with greater efficacy.
- the substance identified as MSB in the present description together with different metal cations in the same formulated as those of Ca, Zn, Mn and of course Cu, improves the assimilation of those metals (and their respective oxides) by part of the plant.
- the formula is applied by root, it stimulates the formation of new roots, which are the most effective for the absorption of these metals of high atomic weight and transport in the xylem (ascending system), while in foliar application it improves the 4
- REPLACEMENT SHEET (RULE 26) absorption metabolism towards the phloem.
- the proposed liquid formulation responds to the composition corresponding to the following qualitative and quantitative formula:
- the size of the CuO nanoparticles involved in the liquid formulation of the present invention is less than 60 nm, and more preferably is in a range between 8 nm and 40 nm.
- Figures 1.1 and 1.2 consisting of micrographs by which the way in which the copper from the formulation of the present invention is dispersed is compared on the surface of a sheet ( Figure 1.1) with respect to the dispersion of copper from a conventional formulation (figure 1.2).
- REPLACEMENT SHEET (RULE 26) they are distributed randomly, while in the treatment with the commercial formulation, the Cu is grouped in the intercellular areas instead of being distributed inside the cell. This confirms the mention made above regarding a greater dispersion of the nanoparticles.
- With the formulation of the invention it was possible to obtain CuO nanoparticles with a size between 8 and 40 n that do not tend to agglomerate, thus increasing their effectiveness.
- Figure 2 shows an example of a photomicrograph, performed with a transmission electron microscope that reproduces the size of the particles and their aggregation.
- copper is necessary in the process of photosynthesis, since it is essential for the respiration of plants and is part of the prosthetic group of numerous proteins such as ascorbic acid, phenol or dexides of oxidases.
- cytochrome It favors the use of nitrogen and protein synthesis, also acting as a stabilizer of chlorophyll and helping to intensify the taste and color of vegetables and flowers.
- it is an essential element, it is also considered a heavy metal that can accumulate in the soil, with the peculiarity that it is hardly biodegradable. This presents a risk, since at acidic pH, copper becomes more soluble and therefore can become toxic to auxiliary fauna.
- the liquid formulation of the invention combines the advantage provided by the small size of the CuO nanoparticles, with the vehicular effect provided by the MSB and the complexing power of the essential plant amino acids, facilitating thus the absorption of copper contributed by the plant. It also produces a greater mobilization of! copper in the plant, allowing its assimilation quickly, efficiently and abundantly and allowing it to reach the place where they should exert their action. In this way, it is possible to optimize the metabolic processes that occur during the different stages of plant development, improving the appearance of the treated plants, activating their self-defense and resistance to biotic and abiotic adverse conditions, thereby allowing to increase the yield and Crop production. At the same time, by using a reduced amount of copper, the accumulation in the soil of this metal is reduced, thus contributing to a reduction of the environmental impact.
- REPLACEMENT SHEET soil through fertirrigation, drip irrigation, sprinkling or distributing it in areas close to the plant in blanket irrigation, or foliar, applying with an atomizer, in the initial stages of crop development, to stimulate root formation new.
- the dose of the formulation of the invention to be used is 2.5 liters per hectare of cultivation and application.
- PRODUCT 2 Invention Formulation
- the 36 plants were arranged in a design of 2 repeating blocks. Each experimental unit consisted of 3 plants considered biological replicas, totaling 6 strains per condition. In the test, 6 different conditions have been compared, as described below.
- REPLACEMENT SHEET (RULE 26) Once the first leaves were developed, e! First treatment of the products evaluated. Two weeks later, the inoculations were performed, infecting the plants with the causative agents of the selected EMVs. After two weeks, the second treatment with the evaluated products was applied and subsequently the plants were maintained for a further 3 months, until they were lifted to assess susceptibility and re-isolate pathogenic fungi.
- the plants of conditions 3, 5 and 6 were inoculated, which were infected with two fungal species causing the EMV; Diplodia seriata (Ds) and Phaeoacremonium minimum (Pm).
- the inoculation method consisted of depositing a small square of culture medium with the mycelium of the fresh and active fungus (7-10 days of growth), after making an incision in the plant with a sterile scalpel. The two fungi were inoculated together between the first and the second yolk. Next, a cotton pad soaked in sterile water was placed and the wounds were sealed with parafilm.
- control conditions 1, 2 and 4 On the plants of control conditions 1, 2 and 4 (called "+ C"), not infected, an incision was also made, placing a piece of sterile agar and sealing with cotton and parafilm as described above.
- An assay of the formulation of the invention was carried out to evaluate its effect against symptoms of mildew. Specifically, a test was carried out with 2 plots that correspond to 2 different lots (FCL98 and VN42), within which 3 treatments have been established (control, formulated invention and comparative formulation). All with 4 repetitions, as shown in the scheme of the test that has been represented in Figure 4 of the attached drawings. Together, the number of plants on which copper treatments have been tested is 1200 plants for each of the lots.
- the formulation of the invention was applied via foliar and the test was carried out during the 2017 campaign by the University of Navarra.
- Severity Average of% affection in the total of the affected leaves.
- the invention is particularly applicable in the industrial sector dedicated to the manufacture and application of products to promote and enhance the self-defense of trees and plants.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2020008639A MX2020008639A (es) | 2018-02-19 | 2018-03-12 | Formulado liquido a base de nanoparticulas de cuo como potenciador de la autodefensa de las plantas y uso del mismo. |
BR112020015709-0A BR112020015709B1 (pt) | 2018-02-19 | 2018-03-12 | Formulação líquida à base de nanopartículas de cuo como potencializadora da autodefesa das plantas e o uso da mesma |
EP18906414.0A EP3756463A4 (en) | 2018-02-19 | 2018-03-12 | LIQUID FORMULATION BASED ON CUO NANOPARTICLES AS A POTENTIALIZER OF PLANT SELF-DEFENSE AND USE OF THIS FORMULATION |
US16/969,608 US11369115B2 (en) | 2018-02-19 | 2018-03-12 | Liquid formulation based on CuO nanoparticles to boost the self-defence of plants and use of same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ESP201830145 | 2018-02-19 | ||
ES201830145A ES2725076B2 (es) | 2018-02-19 | 2018-02-19 | FORMULADO LIQUIDO A BASE DE NANOPARTICULAS DE CuO COMO POTENCIADOR DE LA AUTODEFENSA DE LAS PLANTAS Y USO DEL MISMO. |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2019158786A1 true WO2019158786A1 (es) | 2019-08-22 |
Family
ID=67619275
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/ES2018/070181 WO2019158786A1 (es) | 2018-02-19 | 2018-03-12 | FORMULADO LÍQUIDO A BASE DE NANOPARTÍCULAS DE CuO COMO POTENCIADOR DE LA AUTODEFENSA DE LAS PLANTAS Y USO DEL MISMO |
Country Status (10)
Country | Link |
---|---|
US (1) | US11369115B2 (es) |
EP (1) | EP3756463A4 (es) |
AR (1) | AR114180A1 (es) |
BR (1) | BR112020015709B1 (es) |
CL (1) | CL2020002121A1 (es) |
EC (1) | ECSP20050652A (es) |
ES (1) | ES2725076B2 (es) |
MA (1) | MA51885A (es) |
MX (1) | MX2020008639A (es) |
WO (1) | WO2019158786A1 (es) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080039320A1 (en) * | 2004-11-26 | 2008-02-14 | Harald Kohle | Use Of Menadione |
ES2435069A1 (es) * | 2012-06-13 | 2013-12-18 | Agro Stock S.A. | Formulado líquido enraizante y potenciador de la autodefensa en las plantas y utilización del mismo |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2665686T3 (es) * | 2004-02-26 | 2018-04-26 | Ajinomoto Co., Inc | Fertilizante/revitalizante para plantas |
-
2018
- 2018-02-19 ES ES201830145A patent/ES2725076B2/es active Active
- 2018-03-12 US US16/969,608 patent/US11369115B2/en active Active
- 2018-03-12 MX MX2020008639A patent/MX2020008639A/es unknown
- 2018-03-12 MA MA051885A patent/MA51885A/fr unknown
- 2018-03-12 BR BR112020015709-0A patent/BR112020015709B1/pt active IP Right Grant
- 2018-03-12 EP EP18906414.0A patent/EP3756463A4/en active Pending
- 2018-03-12 WO PCT/ES2018/070181 patent/WO2019158786A1/es unknown
-
2019
- 2019-01-15 AR ARP190100081A patent/AR114180A1/es active IP Right Grant
-
2020
- 2020-08-17 CL CL2020002121A patent/CL2020002121A1/es unknown
- 2020-08-19 EC ECSENADI202050652A patent/ECSP20050652A/es unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080039320A1 (en) * | 2004-11-26 | 2008-02-14 | Harald Kohle | Use Of Menadione |
ES2435069A1 (es) * | 2012-06-13 | 2013-12-18 | Agro Stock S.A. | Formulado líquido enraizante y potenciador de la autodefensa en las plantas y utilización del mismo |
Non-Patent Citations (2)
Title |
---|
BISWAL SKNAYAK AKPARIDA UKNAYAK PL: "Applications of nanotechnology in agriculture and food sciences", IJSID, vol. 2, no. 1, 2012, pages 21 - 36 |
See also references of EP3756463A4 |
Also Published As
Publication number | Publication date |
---|---|
BR112020015709A2 (pt) | 2020-12-08 |
ES2725076A1 (es) | 2019-09-19 |
CL2020002121A1 (es) | 2020-12-04 |
MX2020008639A (es) | 2020-09-21 |
EP3756463A4 (en) | 2021-11-10 |
ECSP20050652A (es) | 2020-11-30 |
US20210015104A1 (en) | 2021-01-21 |
US11369115B2 (en) | 2022-06-28 |
BR112020015709B1 (pt) | 2022-10-25 |
AR114180A1 (es) | 2020-07-29 |
EP3756463A1 (en) | 2020-12-30 |
ES2725076B2 (es) | 2020-07-22 |
MA51885A (fr) | 2021-05-26 |
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