WO2019131606A1 - Dispositif d'inspection - Google Patents

Dispositif d'inspection Download PDF

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Publication number
WO2019131606A1
WO2019131606A1 PCT/JP2018/047515 JP2018047515W WO2019131606A1 WO 2019131606 A1 WO2019131606 A1 WO 2019131606A1 JP 2018047515 W JP2018047515 W JP 2018047515W WO 2019131606 A1 WO2019131606 A1 WO 2019131606A1
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WIPO (PCT)
Prior art keywords
absorber
flow path
liquid
inspection device
filter medium
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PCT/JP2018/047515
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English (en)
Japanese (ja)
Inventor
則彦 大河内
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大日本印刷株式会社
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Publication of WO2019131606A1 publication Critical patent/WO2019131606A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass

Definitions

  • the present disclosure relates to an inspection device comprising a microchannel and an absorber.
  • Immunoassays using antigen-antibody reactions occupy an important place in clinical examinations.
  • a sample test such as an immunological test is performed at a site of medical treatment or medical examination
  • a small test device is used to obtain a result on the spot and use it for treatment or prescription.
  • An immunochromatographic method is known as an immunoassay.
  • an immunochromatographic test kit is widely used in detection of viral infections such as influenza, myocardial infarction markers, and the like.
  • the immunochromatographic method has low detection sensitivity, it has been insufficient in cases where high-sensitivity tests such as, for example, early diagnosis of infectious disease and early detection of cancer are required.
  • an enzyme-linked immunosorbent assay (ELISA: Enzyme-Linked Immunosorbent Assay) method is also known.
  • the ELISA method has the advantage of being capable of highly sensitive detection and of excellent quantitativity.
  • the ELISA method requires a long time for the operation time and the measurement time, and the measurement operation is complicated, so that it is difficult to use for a simple and quick test. Therefore, in the ELISA method, development of a test device capable of a simple and quick test has been actively carried out.
  • Patent Document 1 discloses an assay device, more specifically, a microfluidic device using a porous medium, specifically, a microchannel having a tip and a vicinity of the tip of the microchannel And a space disposed between the microchannel and the porous medium, and the fluid moving in the microchannel based on the lateral flow is porous over the space.
  • An assay device is proposed that is configured to be separated in space so that fluid may be placed in the microchannel after being absorbed in contact with the quality medium.
  • Patent Document 1 discloses that, in an assay device, a space disposed between a microchannel and a porous medium (absorbent paper) functions as a valve mechanism to cause a plurality of liquids to flow repeatedly and stop repeatedly. It is stated that multistep assays can be performed.
  • air vents are typically provided and placed on top of absorbent paper, as also described in US Pat.
  • the air vent hole is blocked by the absorbent paper, and therefore, once the absorbent paper absorbs the liquid moving in the microchannel, the air vent hole It becomes difficult to get out of it. Therefore, when the liquid flows in the flow path, the air from the flow path is difficult to drop out of the air vent hole, so the liquid hardly flows in the flow path, and the liquid may be stopped halfway in the flow path. Therefore, it is difficult to flow a plurality of liquids continuously.
  • the present disclosure has been made in view of the above circumstances, and it is an object of the present disclosure to provide an inspection device capable of simple and rapid inspection and capable of continuously flowing a plurality of liquids. .
  • the present disclosure provides a first base, a second base facing the first base, a flow path between the first base and the second base, and one end of the flow path A liquid holding portion connected to the other end, for introducing a liquid, connected to the other end of the flow path, and arranged with an absorber, an absorber arranged in the liquid holding portion, the flow path and the flow path And an air port disposed between the absorbers and connected to the flow path.
  • the present disclosure has the effect of being able to provide an inspection device capable of simple and quick inspection, and capable of continuously flowing a plurality of liquids.
  • FIG. 1 is a schematic plan view and a cross-sectional view showing an example of the inspection device of the present disclosure. It is the schematic plan view and sectional drawing which show the other example of the inspection device of this indication. It is the schematic plan view and sectional drawing which show the other example of the inspection device of this indication. It is the schematic plan view and sectional drawing which show an example of the conventional inspection device. It is a schematic sectional drawing which shows the other example of the inspection device of this indication. It is process drawing which shows an example of the usage method of the inspection device of this indication. It is the schematic plan view and sectional drawing which show the other example of the inspection device of this indication. It is the schematic plan view and sectional drawing which show the other example of the inspection device of this indication. It is the schematic plan view and sectional drawing which show the other example of the inspection device of this indication.
  • the inspection device of the present disclosure is connected to a first base, a second base facing the first base, a flow path between the first base and the second base, and one end of the flow path, A liquid holding unit connected to the other end of the flow path and arranged at the other end of the flow path, an absorbent body arranged in the liquid holding portion, and the space between the flow path and the absorbent body And an air port disposed and connected to the flow path.
  • FIG. 1 is a schematic plan view and a cross-sectional view showing an example of the inspection device of the present disclosure
  • FIG. 1 (b) is a cross-sectional view taken along line AA of FIG. 1 (a).
  • the inspection device 1 includes a first base 2, a second base 3 facing the first base 2, and a space between the first base 2 and the second base 3.
  • the flow path 4 and one end of the flow path 4 are connected to the inlet 5 for introducing the liquid, and the other end of the flow path 4 is connected to the liquid holding portion 7 in which the absorber 10 is disposed; It has the absorber 10 arrange
  • the inspection device 1 can have an introduction part 6 for introducing a liquid between the introduction port 5 and the flow path 4.
  • the inspection device 1 can also have a spacer 12 for forming the flow path 4 between the first base 2 and the second base 3. In FIG. 1A, the flow path 4 is shown by a broken line because it is covered by the second base 3.
  • Fig. 2 is a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure
  • Fig. 2 (b) is a cross-sectional view taken along line AA of Fig. 2 (a);
  • Fig. 2 is a plan view of the absorber in Fig. 2 (a).
  • the absorber 10 can be disposed from the inside of the liquid holding unit 7 to the inside of the flow path 4.
  • the absorber 10 can have a protrusion 10 a disposed in the flow path 4 as shown in FIG. 2 (c).
  • symbol which is not demonstrated in FIG. 2, since it shows what is the same member as FIG. 1, description here is abbreviate
  • an air port is disposed between the flow path and the absorber as in the case of the inspection device shown in FIGS. 1 (a) and 1 (b) above.
  • Fig. 3 is a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure
  • Fig. 3 (b) is a cross-sectional view taken along the line AA of Fig. 3 (a);
  • Fig. 3 is a plan view of a first absorber of the absorber in Fig. 3 (a).
  • the absorber 10 is disposed only in the first absorber 8 disposed in the flow channel 4 and in the liquid holding unit 7, And a second absorber 9 in contact with the first absorber 8.
  • the second absorber 9 is not in contact with the flow path 4.
  • the first absorbent body 8 may be disposed from the inside of the liquid holding unit 7 to the inside of the flow path 4.
  • the first absorber 8 can have a projection 8a disposed in the flow path 4 as shown in FIG. 3 (c).
  • symbol which is not demonstrated in FIG. 3, since it shows what is the same member as FIG. 1, description here is abbreviate
  • an air port is disposed between the flow path and the absorber as in the case of the inspection device shown in FIGS. 1 (a) and 1 (b) above.
  • the air port is disposed between the flow path and the absorber” means that the liquid holding portion is not filled with the absorber, and the flow is performed at the end portion on the liquid holding portion side of the flow path.
  • An air gap exists between the passage and the absorber, and the air gap is said to be open to the outside.
  • the air port 11 when the air port 11 is disposed on the upper surface of the absorber 10 in the inspection device 100, the air port 11 is blocked by the absorber 10. In this case, once the absorber absorbs the liquid, the air is less likely to pass through the absorber, so the air in the flow path is less likely to escape out. Therefore, for example, when the first liquid and the second liquid are made to flow continuously, after the absorber absorbs the first liquid, the air pushed out when the second liquid moves in the flow path is Since the second liquid does not easily flow out of the air port, it may be difficult for the second liquid to flow in the flow path, and the second liquid may stop in the middle of the flow path. Therefore, it becomes difficult to flow a plurality of liquids continuously.
  • FIG. 4 (b) is a cross-sectional view taken along line AA of FIG. 4 (a).
  • the test device of the present disclosure can be suitably used for a test that utilizes multiple steps of antigen-antibody reaction such as ELISA. Furthermore, it is possible to carry out the inspection easily and quickly without requiring a pump or the like.
  • An absorbent body in the present disclosure is a member that is disposed in the liquid holding unit and absorbs the liquid that has moved in the flow path.
  • the absorber may be arranged in the liquid holding unit and the air port may be arranged between the flow path and the absorber, for example, as shown in FIG.
  • the absorber 10 may be disposed only in the liquid holding portion 7, and FIGS. 2 (a), (b) and 3 (a), (b)
  • the absorber 10 may be disposed from the inside of the liquid holding portion 7 to the inside of the flow path 4 as shown in FIG.
  • the absorber be disposed from the inside of the liquid holding portion to the inside of the flow path.
  • the air port is disposed between the flow path and the absorber
  • the flow path and the absorber are separated by the gap between the flow path and the absorber, and the inside of the flow path is The liquid which has moved may be difficult to be absorbed by the absorber.
  • the width of the space portion is made the same as the width of the microchannel, and the height of the space portion is made micro, for the space portion disposed between the microchannel and the porous medium (absorbent paper).
  • the height of the space is the same as the height of the microchannel, the height of the space is limited to the height of the microchannel, and as a result, the thickness of the absorbent paper is equal to the height of the microchannel It may be limited and the absorption capacity of the absorbent paper may run short.
  • the absorber is disposed from the inside of the liquid holding portion to the inside of the flow path, that is, when it is inserted in the flow path, the liquid moving in the flow path is reliably absorbed by the absorber be able to. Therefore, even when there is a step between the flow path and the liquid holding portion, it is possible to prevent the liquid from being stopped by the step.
  • positioned in the liquid holding part of an absorber does not receive the restriction
  • the absorber 10 when the absorber is disposed only in the liquid holding unit, it is preferable that the absorber 10 be in contact with the flow path 4 as shown in, for example, FIGS. 1 (a) and 1 (b).
  • the flow path and the absorber are separated by the gap between the flow path and the absorber, and the flow path It may be difficult for the liquid moving inside to be absorbed by the absorber.
  • the absorber moving in the flow path is reliably absorbed by the absorber because the absorber is in contact with the flow path. be able to.
  • the absorber is disposed only in the fluid holding portion and is not in contact with the flow path, as described later, movement in the flow path is achieved by enhancing the hydrophilicity and the like of the bottom of the flow path. It is possible for the absorbent to absorb the liquid that has been absorbed.
  • the absorber 10 may be, for example, a single member as shown in FIGS. 1 (a) and 1 (b) and FIGS. 2 (a) and 2 (b), as shown in FIGS. 3 (a) and 3 (b). As shown, it may have the first absorber 8 and the second absorber 9.
  • the first absorber and the second absorber may be in contact with each other, for example, as shown in FIGS. 3 (a), (b) and 5 (a).
  • the first absorber 8 and the second absorber 9 may be stacked, and as shown in FIG. 5 (b), the first absorber 8 and the second absorber 9 are arranged in parallel. May be
  • the first absorber 8 and the second absorber 9 be stacked in this order. This is because the first absorber and the second absorber can be reliably in contact with each other, and the second absorber can reliably absorb the liquid absorbed by the first absorber.
  • the first absorber and the second absorber are laminated, for example, as shown in FIGS. 3A and 3B, one end of each of the first absorber 8 and the second absorber 9 is They may be arranged to be aligned, and as shown in FIG. 5 (a), the first absorber 8 and the second absorber 9 may be stacked in a mutually offset manner.
  • the absorber absorbs liquid and has a predetermined water absorbency.
  • the water absorptivity of the absorber is appropriately selected according to the configuration of the absorber and the like.
  • the absorber 10 when the absorber 10 is disposed only in the liquid holding unit 7, the absorber preferably has high water absorbency. Since the absorption capacity of the absorbent can be increased by the absorbent having high water absorbency, it is suitable for continuously flowing a plurality of liquids.
  • the water absorptivity of the absorber is not particularly limited as long as it is substantially the same as the water absorptivity of the absorber generally used for microfluidic devices, but for example, the Klemm water absorption is preferably 10 mm or more, and in particular, 50 mm It is preferable that it is more than. Further, the Klemm water absorption is preferably 200 mm or less, and more preferably 150 mm or less.
  • Klemm water absorption is too low, it takes time for the liquid to absorb water, and the amount of absorbed water also decreases, which may make it difficult to continuously flow a plurality of liquids. Also, if the Klemm water absorption is too high, no liquid will remain in the channel, that is, as will be described later, the liquid may not be separated into two and all may be absorbed by the absorber, so the antigen in the channel It may be difficult to carry out antibody reaction or enzyme reaction.
  • Klemm water absorption is defined as "the height (mm) which water immersed in water vertically by the lower end of the paper and increased in 10 minutes by capillary action". Further, the Klemm water absorption can be measured in accordance with JIS P 8141: 2004 (paper and paper board-water absorption test method-Klemm method).
  • the absorber 10 is disposed from the inside of the liquid holding portion 7 to the inside of the flow path 4
  • the absorber 10 includes the first absorber 8 disposed in the flow path 4 and the second absorber 9 disposed only in the liquid holding unit 7 and in contact with the first absorber 8;
  • the second absorbent body 9 is not in contact with the flow path 4, it is preferable that the water absorbency of the first absorbent body is lower than the water absorbability of the second absorbent body.
  • the first liquid 31 is introduced into the introduction part 6 from the introduction port 5
  • the first liquid 31 is drawn into the flow path 4 by capillary action by the flow path 4.
  • the flow path 4 is moved by the gravity and the capillary action applied to the first liquid, and is absorbed by the absorber 10.
  • the first liquid 31 flows until the first liquid 31 disappears in the introduction part 6, and is absorbed by the absorber 10.
  • the first liquid 31 leaves the absorber 10 and slightly reverses and stops. As a result, the first liquid 31 is separated into two, one in the absorber 10 and the other in the flow path 4.
  • the second liquid located higher than the flow path in the introduction part acts as if it were a weight and the first liquid Push the back end.
  • the pushed first liquid starts moving in the flow path toward the liquid holding portion and immediately reaches the absorber.
  • the first liquid reaching the absorber is absorbed by the absorber, and the first liquid flows in the flow path until the second liquid disappears in the introduction part, and is absorbed by the absorber.
  • the second liquid 32 flows until the second liquid 32 disappears in the introduction part 6, and is absorbed by the absorber 10.
  • the second liquid 32 leaves the absorber 10 and slightly reverses and stops. At this time, if a sufficient amount of the second liquid is introduced, the first liquid remaining in the flow path is completely replaced by the second liquid.
  • the absorber is disposed from the inside of the liquid holding portion to the inside of the flow path, and the absorber is disposed only in the first absorption body disposed in the flow path and the liquid holding portion, and is in contact with the first absorption body
  • the test device of the present disclosure when the body has a second absorbent body not in contact with the flow path and the water absorptivity of the first absorbent body is lower than the water absorptivity of the second absorbent body; The reason why the flow of liquid separates into two is not clear, but is considered as follows.
  • the second absorbent having high water absorbability is disposed only in the liquid holding unit and is not in contact with the flow channel, while the first absorbent having low water absorbability is disposed in the flow channel. Therefore, it is considered that the liquid moving in the flow path first contacts the first absorber, and then contacts the second absorber via the first absorber.
  • a force in a direction higher than the flow path in the introduction portion from the flow path in the direction from the flow path to the liquid holding portion The force of the atmospheric pressure applied from the inlet and the force of absorbing the liquid by the absorber work, while the force of the atmospheric pressure applied from the air port works in the direction of the flow path from the liquid holding portion.
  • the force applied from the flow path in the direction of the liquid holding portion is larger than the force applied from the liquid holding portion in the direction of the flow path. You can reach to Thereafter, when the liquid disappears in the introduction portion, the force pressing the liquid in the flow path disappears.
  • the water absorbency of the first absorber is sufficiently low, it is considered that the force applied from the liquid holding portion in the direction of the flow path is more dominant than the force applied from the flow path in the direction of the liquid holding portion.
  • the force due to the atmospheric pressure from the inlet and the force due to the atmospheric pressure from the air port become balanced and stop.
  • the absorber is disposed from the inside of the liquid holding portion to the inside of the flow path, and the absorber is disposed only in the first absorption body disposed in the flow path and the liquid holding portion, and And the second absorbent is not in contact with the flow path, and the water absorption of the first absorbent is lower than the water absorption of the second absorbent, the flow path You can let the liquid stay inside. Therefore, there is no concern that liquid will be lost in the channel during the antigen-antibody reaction or enzyme reaction, and liquid exchange can be performed reliably. Therefore, for example, it is particularly suitable for a test using multistep antigen-antibody reaction such as ELISA.
  • the water absorption of the first absorber may be lower than the water absorption of the second absorber, but among them, it is preferable to have low water absorption or non-water absorption, and in particular, to have low water absorption. Is preferred.
  • the water absorption of the first absorbent may be low water absorption or non water absorption, but specifically, it is preferable that the Klemm's water absorption be less than 5 mm. If the water absorption of the first absorbent body is in the above range, the force of the first absorbent body to absorb the liquid can be weakened, and the liquid is separated into two and the liquid is allowed to stay in the flow path. It is because
  • the water absorbency of the second absorbent may be higher than the water absorbency of the first absorbent, but among them, it is preferable to have high water absorbency. Since the second absorbent has high water absorbability, the absorption capacity of the second absorbent can be increased, which is suitable when the plurality of liquids are made to flow continuously.
  • the water absorptivity of the second absorber is not particularly limited as long as it is substantially the same as the water absorptivity of an absorber generally used for a microfluidic device, but for example, it is preferable that the Klemm's water absorbency is 10 mm or more Among them, 50 mm or more is preferable.
  • a Klemm water absorption degree is 200 mm or less, and it is preferable that it is 150 mm or less especially. If the water absorption of the second absorbent body is too low, the absorption capacity of the second absorbent body may be insufficient or the water absorption rate may be reduced. In addition, if the water absorption of the second absorbent is too high, the second absorbent is likely to swell, which may block the air port between the flow path and the absorbent.
  • the water absorbability of the absorber Is not particularly limited.
  • the absorber may have high water absorption or low water absorption.
  • the absorber preferably has low water absorbency.
  • the absorber is disposed from the inside of the liquid holding portion to the inside of the flow passage and is a single member, and the absorber has low water absorbency, the fluid moved in the flow passage is absorbed After being absorbed by the body, it is divided into two at the interface with the absorber in the channel, one in the absorber and the other in the channel. Therefore, the liquid can be retained in the flow channel, which is advantageous for performing an antigen-antibody reaction, an enzyme reaction, exchange of the liquid, and the like.
  • the absorbency of the absorber may be low water absorption, but specifically, it is preferable that the Klemm water absorption be less than 5 mm. If the Klemm water absorption is in the above-mentioned range, the force of absorbing the liquid by the absorber can be weakened, and the liquid can be separated into two and the liquid can be retained in the flow path.
  • an absorber As a material of an absorber, it selects suitably according to the structure etc. of an absorber.
  • the material of the absorbent body when the absorbent body 10 is disposed only in the liquid holding portion 7, the material of the absorbent body can obtain an absorbent body having high water absorption.
  • Material is preferable.
  • the material of such an absorber may be the same as the material of the absorber generally used for microfluidic devices, and is not particularly limited.
  • the absorber 10 is disposed from the inside of the liquid holding portion 7 to the inside of the flow path 4
  • the absorber 10 includes the first absorber 8 disposed in the flow path 4 and the second absorber 9 disposed only in the liquid holding unit 7 and in contact with the first absorber 8;
  • the material of the first absorbent body may be any material capable of obtaining a first absorbent body having a lower water absorbency than the second absorbent body.
  • a material capable of obtaining a first absorbent having low water absorption or non-water absorption is preferable, and in particular, a material capable of obtaining a first absorbent having low water absorption Is preferred.
  • a material of such a 1st absorber vegetable fiber, animal fiber, a synthetic fiber, glass fiber, mineral fiber, metal fiber etc. are mentioned, for example.
  • vegetable fibers are preferred.
  • tracing paper, glassine paper, medicine packaging paper, sulfuric acid paper, paraffin paper or the like can be used. Because these papers are processed to reduce the gaps between plant fibers, their Klemm water absorption is usually less than 5 mm.
  • the resin base material can also be used, for example.
  • the resin constituting the resin base examples include polyethylene terephthalate (PET), polyvinyl alcohol, cellophane, cellulose resin, polyvinyl acetate, polyvinyl formal, polyvinyl butyral, nylon, polyvinyl chloride, styrene copolymer, methacrylic resin, polycarbonate And urea resin, phenol resin, melamine resin, polyacetal and the like.
  • PET polyethylene terephthalate
  • polyvinyl alcohol cellophane
  • cellulose resin polyvinyl acetate
  • polyvinyl formal polyvinyl formal
  • polyvinyl butyral nylon
  • polyvinyl chloride polyvinyl chloride
  • styrene copolymer methacrylic resin
  • the surface of the resin base may be subjected to a hydrophilization treatment.
  • the material of the second absorbent may be any material capable of obtaining a second absorbent having higher water absorbability than the first absorbent, but above all, the second absorbent having high water absorbability It is preferable that it is the material which can obtain.
  • a material of such a 2nd absorber it can be made to be the same as that of the material which can obtain the absorber which has the above-mentioned high water absorption.
  • a material of the absorber Is not particularly limited, and may be, for example, a material capable of obtaining an absorbent having high water absorption, and even a material capable of obtaining an absorbent having low water absorption.
  • the material of the absorber is preferably a material capable of obtaining an absorber having low absorbency.
  • the material capable of obtaining an absorbent having high water absorption is as described above.
  • a material which can obtain the absorber which has low absorbency it can be made to be the same as the material which can obtain the above-mentioned 1st absorber which has low water absorbency.
  • the dimensions and shape of the thickness, width, length and the like of the absorber are appropriately selected according to the configuration and the like of the absorber.
  • the thickness of the absorber is not particularly limited. It is preferred that the height is greater than the height. This is because the absorption capacity of the absorber can be increased. Therefore, it is suitable when flowing a plurality of liquids continuously.
  • the width of the absorber is not particularly limited as long as it is equal to or less than the width of the liquid holding portion, but it is preferable that the width is the same as the width of the liquid holding portion. This is because the absorption capacity of the absorber can be increased. Therefore, it is suitable when flowing a plurality of liquids continuously.
  • the "width of the absorber” refers to the length in the width direction of the flow path of the absorber. The same applies to “width of first absorber” and “width of second absorber”. Further, “the width of the liquid holding portion” refers to the length in the width direction of the flow path of the liquid holding portion.
  • the length of the absorber is not particularly limited as long as it is equal to or less than the length of the liquid holding portion, but among them, the maximum length of the absorber is liquid so that the absorber can contact the flow path. It is preferable that it is the same as the length of a holding part.
  • the length of an absorber means the length of the length direction of the flow path of an absorber. The same applies to “the length of the first absorber” and “the length of the second absorber”.
  • the length of the liquid holding portion refers to the length of the flow path of the liquid holding portion in the longitudinal direction.
  • the shape of the absorber is not particularly limited as long as an air port can be disposed between the flow passage and the absorber, and the absorber can be in contact with the flow passage.
  • the cross-sectional shape in the longitudinal direction of the flow path of the absorber can be a shape in which the surface on the flow path 4 side of the absorber 10 is inclined as shown in FIG.
  • the volume of the absorber is not particularly limited, and the thickness, width, and length of the above-described absorber, and the amount of liquid used when performing the inspection using the inspection device of the present disclosure It is set appropriately according to
  • the absorber 10 is disposed from the inside of the liquid holding portion 7 to the inside of the flow path 4
  • the absorber 10 includes the first absorber 8 disposed in the flow path 4 and the second absorber 9 disposed only in the liquid holding unit 7 and in contact with the first absorber 8;
  • the cross-sectional area of the cross direction of the channel of the 1st absorber is smaller than the cross-sectional area of a channel.
  • an air port can be disposed between the flow passage and the absorber, and a passage of air which is pushed out when the liquid moves in the flow passage can be secured.
  • the first absorber can be easily inserted into the flow path.
  • the thickness of the first absorber may be smaller than the height of the flow path
  • the width of the body protrusion may be smaller than the width of the flow channel.
  • the protrusion part of a 1st absorber is mentioned later.
  • the magnitude of the force by which the first absorber sucks the liquid is affected by, for example, the contact area between the liquid and the first absorber, that is, by the cross-sectional area in the width direction of the flow path of the first absorber. Therefore, the thickness of the first absorber and the width of the protrusions of the first absorber can be appropriately adjusted so that the force of the first absorber to absorb the liquid has a desired magnitude.
  • the thickness of the first absorber may be equal to or less than the height of the flow path, and may be, for example, 0.2 mm or less, and may be 0.1 mm or less.
  • the thickness can be, for example, 0.01 mm or more, and may be 0.05 mm or more.
  • the first absorber 8 can have a projection 8 a disposed in the flow path 4.
  • the shape of the first absorber is not particularly limited as long as the first absorber can be disposed in the flow path. Among them, as described above, the plan view shape of the first absorber can be a shape having a projection.
  • the plan view shape of the projection is not particularly limited as long as the projection of the first absorber can be inserted into the flow path.
  • a rectangular shape as shown in FIG. 7 (c) a triangular shape as shown in FIG. 7 (d), a semicircular shape or a semielliptical shape as shown in FIG. 7 (e), and the like.
  • a tapered shape such as a trapezoidal shape, a triangular shape, a semicircular shape, or a semielliptical shape is preferable because the protrusion can be easily inserted into the flow channel.
  • 7A and 7B are a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure, and FIG.
  • FIG. 7B is a cross-sectional view taken along the line AA of FIG. )
  • To (e) are plan views of the first absorber of the absorber in FIG. 7 (a).
  • the plan view shape of the protrusion is the first Any shape may be used as long as the protrusion of the absorber can be inserted into the flow path, and may be the same as or different from the plan view shape of the enlarged portion of the flow path, for example.
  • 8 to 11 are a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure.
  • 8 (b) is a cross-sectional view taken along the line AA of FIG. 8 (a)
  • FIG. 8 (c) is a plan view of a first absorber of the absorber in FIG. 8 (a).
  • FIG. 9 (b) is a sectional view taken along the line AA of FIG. 9 (a)
  • FIG. 9 (c) is a plan view of a first absorber of the absorber in FIG. 9 (a).
  • 10 (b) is a cross-sectional view taken along the line AA of FIG. 10 (a)
  • FIG. 10 (c) is a plan view of a first absorber of the absorber in FIG. 10 (a).
  • 11 (b) is a cross-sectional view taken along the line AA of FIG. 11 (a)
  • FIG. 11 (c) is a plan view of a first absorber of the absorber in FIG. 11 (a).
  • FIG. 9 shows an example in which the protrusion 8a of the first absorber 8 is disposed in the flow passage 4 beyond the enlarged portion 14, and the shape of the protrusion 8a in plan view is different from the shape of the enlarged portion 14 in plan It is.
  • the unexplained reference numerals in FIGS. 8 to 11 indicate the same members as those in FIG. 1, and thus the description thereof is omitted here.
  • FIG. 12 is a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure.
  • FIG. 12 (b) is a cross-sectional view taken along the line AA of FIG. 12 (a), and FIG. 12 (c) is a plan view in which the second base and the second absorber are omitted in FIG. 12 (a).
  • the width of the projection of the first absorber is appropriately set according to the presence or absence of the enlarged portion of the flow passage, the plan view shape of the projection, and the like.
  • the width of the protrusion is not particularly limited as long as it is equal to or less than the width of the flow path.
  • the maximum width of the projection is preferably the same as the width of the flow channel.
  • the length of the portion disposed in the flow path of the first absorber is, for example, 0.25 or more, where the width of the flow path is 1. And preferably 0.5 or more. Further, the ratio can be, for example, 5 or less, and preferably 2.5 or less. Specifically, the length of the portion disposed in the flow path of the first absorber, that is, the length of the protrusion of the first absorber can be 0.5 mm or more, and 1 mm or more Is preferred. Moreover, the said length can be 10 mm or less, and it is preferable that it is 5 mm or less.
  • the length of the protrusion of the first absorber is too small, it may be difficult to arrange the first absorber from the inside of the liquid holding portion to the inside of the flow path. If the length of the protrusion of the first absorber is too large, it may be difficult to insert the protrusion of the first absorber into the flow channel.
  • the width of the portion other than the protrusion of the first absorbent body is not particularly limited as long as it is equal to or less than the width of the liquid holding portion, but it is preferable that the width is the same as the width of the liquid holding portion.
  • the first absorber and the second absorber be stacked in this order, and the width of the portion other than the protrusion of the first absorber be the same as the width of the liquid holding unit
  • the first absorber and the second absorber can be stably stacked.
  • the length of the portion other than the protruding portion of the first absorber is not particularly limited as long as it is equal to or less than the length of the liquid holding portion, but it is preferable that the length is the same as the length of the liquid holding portion. As described above, in the liquid holding portion, it is preferable that the first absorber and the second absorber are stacked in this order, and the length of the portion other than the protrusion of the first absorber is the same as the length of the liquid holding portion If so, the first absorber and the second absorber can be stably laminated.
  • the thickness of the second absorber is not particularly limited. Since the second absorber is disposed only in the liquid holding portion, the height of the flow path is not limited. Therefore, for example, the thickness of the second absorber can be increased to increase the absorption capacity of the second absorber. Thus, by adjusting the thickness of the second absorber and adjusting the absorption capacity of the second absorber, it is possible to cope with the increase or decrease of the amount of liquid used for the inspection.
  • the thickness of the second absorber may be, for example, 0.1 mm or more, and may be 0.2 mm or more. The thickness may be 1 mm or less, and may be 0.5 mm or less.
  • the width of the second absorber is not particularly limited as long as it is equal to or less than the width of the liquid holding portion, but it is preferable that the width be equal to the width of the liquid holding portion. This is because the absorption capacity of the second absorber can be increased. Therefore, it is suitable when flowing a plurality of liquids continuously.
  • the length of the second absorbent is not particularly limited as long as it is equal to or less than the length of the liquid holding portion, but is preferably less than the length of the liquid holding portion so that the second absorbent does not contact the flow path.
  • the shape of the second absorber is not particularly limited as long as an air port can be disposed between the channel and the absorber, and the second absorber can be prevented from coming into contact with the channel. It is not a thing.
  • the volume of the second absorber is not particularly limited, and the thickness, width, and length of the above-mentioned second absorber, and the amount of liquid used when performing the inspection using the inspection device of the present disclosure. It is set appropriately according to
  • the absorber 10 in the case where the absorber 10 is disposed from the inside of the liquid holding portion 7 to the inside of the flow passage 4 and is a single member, the dimensions and dimensions of the absorber
  • the shape may be the same as the size and shape of the first absorber described above.
  • Air port in the present disclosure is arranged to connect to the flow path, and is arranged between the flow path and the absorber so as to let out the air pushed out as the liquid travels in the flow path. It is a part provided in
  • the arrangement position of the air port is not particularly limited as long as the air port is arranged to connect to the flow path and is arranged between the flow path and the absorber.
  • the air port may be disposed on the top surface of the inspection device or may be disposed on the side surface of the inspection device. That is, in the inspection device, when the first base is disposed on the bottom side of the flow path and the second base is disposed on the top side of the flow path, the air port may be disposed on the second base And may be disposed in a spacer described later. That is, the second base may have an air port, and the spacer may have an air port.
  • the plan view shape of the air port is not particularly limited, and examples thereof include a rectangular shape, a linear shape, a triangular shape, and a circular shape.
  • the length in the longitudinal direction of the flow path of the air port can be set arbitrarily, and can be, for example, 10 ⁇ m or more.
  • the length may be, for example, 1 mm or less, and may be 100 ⁇ m or less. If the said length of an air port is in the said range, several liquids can be made to flow continuously.
  • the area of the air port in plan view can be arbitrarily set, and can be, for example, 0.1 mm 2 or more. Further, the area may be, for example, 10 mm 2 or less, and may be 1 mm 2 or less. If the area of the air port in plan view is within the above range, a plurality of liquids can be made to flow continuously.
  • Examples of the method for forming the air port include punching and laser processing.
  • the flow path in the present disclosure is a flow path disposed between the first base and the second base, one end connected to the inlet, and the other end connected to the liquid holding unit, and a flow causing capillary action. It is a road.
  • the flow path may be disposed between the first base and the second base, one end of which is connected to the inlet, and the other end of which is connected to the liquid holding unit.
  • the bottom surface of the flow path and the bottom surface of the introduction portion described later be continuous, and there is no step between the bottom surface of the flow path and the bottom surface of the introduction portion. This is because the liquid can be quickly drawn into the flow path from the introduction portion.
  • the bottom surface of the flow path and the bottom surface of the liquid holding portion be continuous, and there is no level difference between the bottom surface of the flow path and the bottom surface of the liquid holding portion. This is because the absorber can be easily disposed from the inside of the liquid holding portion to the inside of the flow path.
  • At least one of the bottom surface and the top surface of the flow channel has hydrophilicity.
  • the liquid easily wets and spreads, the liquid is easily drawn into the flow path by capillary action, and the liquid easily flows in the flow path.
  • the bottom surface of the flow channel may have hydrophilicity
  • the top surface may have hydrophilicity, and among them, it is preferable that the bottom surface and the top surface have hydrophilicity.
  • the side surface of the flow path also be hydrophilic.
  • hydrophilic means that the static contact angle of water on a predetermined surface is 60 degrees or less.
  • the static contact angle of water on a given surface is preferably 30 degrees or less.
  • the static contact angle of water on a predetermined surface is usually 3 degrees or more.
  • the static contact angle of water is such that a drop of 1.0 ⁇ L of pure water is dropped on the surface of the measurement object, and one second after landing, the angle of the straight line connecting the left and right end points of the dropped drop to the solid surface
  • the contact angle is measured according to the ⁇ / 2 method for calculating the contact angle from As a measuring device, for example, a contact angle meter DM500 manufactured by Kyowa Interface Science Co., Ltd. can be used.
  • the flow path 4 can have an enlarged portion 14 at the end on the liquid holding portion 7 side.
  • the enlarged portion is a portion where the cross-sectional area of the flow path increases toward the liquid holding portion.
  • the absorber can be easily inserted into the channel by having the enlarged portion at the end on the liquid holding unit side of the channel.
  • the plan view shape of the enlarged portion of the flow path is not particularly limited, and, for example, a trapezoidal shape as shown in FIGS. 8 (a), 9 (a) and 12 (a), FIG. 10 (a) And a semicircular shape or a semielliptical shape as shown in FIG. 11 (a).
  • a tapered shape such as a trapezoidal shape, a semicircular shape or a semielliptical shape is preferable because the absorber can be easily inserted into the flow channel.
  • the reagents 21 and 22 are disposed. It can have reagent arrangement parts 21S and 22S.
  • the reagent placement portion is a region in which the reagent is fixed in the flow path.
  • the arrangement position of the reagent arrangement unit is not particularly limited, and may be, for example, an intermediate position of the flow path.
  • the flow path may have one reagent placement part or may have a plurality of reagent placement parts.
  • the number of reagent placement parts is appropriately selected according to the number of reagents, the application of the test device, and the like.
  • the plan view shape of the reagent disposition part is not particularly limited, and examples thereof include a circular shape, an elliptical shape, a rectangular shape, and a rhombus shape.
  • the plan view shape of the flow path is not particularly limited, and may be, for example, a linear shape or a serpentine shape.
  • the size of the inspection device can be reduced, which is preferable.
  • the width of the flow channel may be such a degree that capillary action appears, and can be, for example, 0.1 mm or more, and 5 mm or less. Further, the width of the flow path may be constant or may be indefinite. For example, in the case where the flow path has an enlarged portion at the end portion on the liquid holding portion side, the enlarged portion can be made wider and the region other than the enlarged portion can be made narrower. In addition, when the flow path has a reagent disposition portion, the reagent disposition portion may be widened, and the region other than the reagent disposition portion may be narrowed.
  • the width of the region other than the enlarged portion and the reagent disposition portion in the flow channel can be 0.1 mm or more, and can be 2 mm or less. If the width of the region other than the enlarged portion and the reagent disposition portion in the flow channel is in the above range, the liquid can stably flow in the flow channel by capillary action. In addition, the width of the enlarged portion in the flow path can be 1 mm or more, and can be 5 mm or less. If the width of the enlarged portion in the flow path is in the above range, the absorber can be easily inserted into the flow path. In addition, the width of the reagent placement portion in the flow channel can be 0.25 mm or more, and can be 5 mm or less. If the width of the reagent placement portion in the flow path is in the above-mentioned range, the liquid can stably flow in the flow path by capillary phenomenon, and the inspection can be performed with high sensitivity.
  • the height of the flow channel may be such a level that capillary action occurs, and can be, for example, 0.05 mm or more, preferably 0.1 mm or more. Moreover, the said height can be 1 mm or less, and 0.5 mm or less is preferable.
  • the length of the flow path is appropriately set according to the type of liquid, the application of the inspection device, and the like, and can be, for example, 5 mm or more, and preferably 25 mm or more. Moreover, the said length can be 1000 mm or less, and it is preferable that it is 500 mm or less. If the length of the flow path is in the above range, the test can be performed with a small amount of sample.
  • the cross-sectional shape in the height direction of the flow channel is usually a rectangular shape, but may be, for example, an arch shape, a trapezoidal shape, or a triangular shape.
  • the height direction of the flow path is the thickness direction of the first base and the second base.
  • the liquid holding unit in the present disclosure is a space that is disposed at an end of the flow channel opposite to the inlet, the absorbent body is disposed, and holds the liquid moving in the flow channel.
  • the bottom surface of the liquid holding portion preferably has hydrophilicity. Thereby, the liquid is easily spread by wetting, and the liquid is easily drawn into the liquid holding portion. Moreover, it is preferable that the top surface and the side surface of the liquid holding portion also have hydrophilicity.
  • the hydrophilicity can be the same as the hydrophilicity described in the section of the flow path.
  • the plan view shape of the liquid holding portion is not particularly limited, and examples thereof include a circular shape, an elliptical shape, a triangular shape, a rectangular shape, a trapezoidal shape, a rhombus shape, and a home base shape.
  • the height of the liquid holding portion can be larger than the height of the flow path.
  • the area in plan view of the liquid holding portion is not particularly limited, and is appropriately set according to the amount of liquid.
  • the inlet in the present disclosure is a portion that is disposed to connect to the flow path and introduces a liquid.
  • the inspection device of the present disclosure can have an introduction part between the introduction port and the flow path.
  • the introduction part is a space which is disposed between the introduction port and the flow path and introduces a liquid.
  • the arrangement position of the introduction port may be the top surface of the introduction portion or a side surface of the introduction portion.
  • the introducing unit preferably has an introducing port on the top surface of the introducing unit. This is because the liquid can be easily dropped to the inlet and the liquid in the flow path can be pushed by the gravity applied to the dropped liquid.
  • the bottom surface of the introduction portion preferably has hydrophilicity.
  • the hydrophilicity can be the same as the hydrophilicity described in the section of the flow path.
  • the shape of the introduction portion on the surface on the introduction port side is not particularly limited, and examples thereof include a circular shape, an elliptical shape, a triangular shape, a rectangular shape, a trapezoidal shape, a rhombus shape, a home base shape and the like.
  • the home base shape can relatively increase the distance from the introduction portion to the flow path from the introduction portion, and as described later, when the filter medium is disposed in the introduction portion, the sample in the introduction portion causes the sample Can be filtered well.
  • the home base shape refers to, for example, the shape of the introducing portion 6 as shown in FIG. Further, as shown in FIG. 1 (a), FIG. 2 (a), FIG.
  • the plan view shape of the introducing part 6 may be a shape having a projection on the flow path 4 side. Also in the shape having a projection on the flow path side, the distance from the introduction portion to the flow path can be made relatively long, and as described later, when the filter medium is disposed in the introduction portion, the introduction is The sample can be well filtered by the filter medium in the part.
  • the height of the introduction part varies depending on the size of the inspection device of the present disclosure and the like, but can be, for example, 0.01 mm or more, preferably 0.1 mm or more. Moreover, the said height can be 1 mm or less, Preferably it is 0.5 mm or less. If the height of the introduction part is in the above range, it is possible to test with a small amount of sample.
  • the area in the surface at the side of the introducing port of an introducing part is not specifically limited, For example, it can be 25 mm ⁇ 2 > or more, and it is preferable that it is 50 mm ⁇ 2 > or more. Moreover, the said area can be 250 mm ⁇ 2 > or less, for example, and it is preferable that it is 100 mm ⁇ 2 > or less. If the area on the surface on the inlet side of the introducing part is in the above range, it is possible to test with a small amount of sample.
  • the inspection device of the present disclosure preferably has a filter medium 15 in the introduction portion 6, as shown in FIG. 13, for example.
  • the filter medium is a member which is disposed in the introduction part and separates the component to be inspected. By disposing the filter medium in the introduction part, the component to be examined of the sample can be separated. Furthermore, the filter medium can prevent air from entering the flow path, and can inhibit inhibition of the antigen-antibody reaction and the enzyme reaction.
  • the filter medium is arranged in the introducing portion, and among them, the filter medium is preferably arranged so as to be in contact with at least the boundary portion between the introducing portion and the flow path.
  • the filter medium since the filter medium is in contact with the boundary between the introduction portion and the flow path, there is no space that is not a flow path between the filter medium and the flow path.
  • the filter medium allows the components to be examined to be separated quickly and easily. Therefore, it is possible to quickly and simply separate the test target component from the small amount of the sample and to test the test target component only by dropping the liquid without adding an external action such as pressure or centrifugal force.
  • the filter medium is preferably in contact with the bottom surface of the introduction portion. Since the filter medium is in contact with the bottom surface of the introduction portion and there is no gap between the filter medium and the bottom surface of the flow path, the capillary phenomenon caused by the flow path makes it easy for the sample having the filter medium to be drawn into the flow path. is there.
  • the filter medium is in contact with the bottom surface of the introduction portion means that the entire bottom surface of the filter medium is in contact with the bottom surface of the introduction portion, and there is a space between the filter medium and the bottom surface of the introduction portion It means that it does not exist. Among them, it is preferable that all of the bottom of the filter medium be in contact with all of the bottom of the introduction part. For example, in FIGS.
  • FIG. 15 a portion indicated by a broken line is a boundary portion 6 ⁇ / b> B between the introduction portion 6 and the flow path 4.
  • FIG. 15 since the reference numerals in FIG. 15 which are not described indicate the same members as in FIG. 1 and FIG. 13, the description here is omitted.
  • the filter medium is preferably in contact with the side surface of the introduction portion. Since the filter medium is in contact with the side surface of the introduction portion and there is no gap between the filter medium and the side surface of the flow path, it is likely that the sample having permeated the filter medium is drawn into the flow path by capillary action by the flow path. is there.
  • the filter medium is in contact with the side surface of the introduction portion means that all the side surfaces of the filter medium are in contact with the side surface of the introduction portion, and there is a space between the filter medium and the side surface of the introduction portion It means that it does not exist. Among them, it is preferable that all the side surfaces of the filter medium be in contact with all the side surfaces of the introduction portion. For example, in FIGS.
  • the introduction part may be filled with the filter medium, and may not be filled with the filter medium.
  • filled with the filter medium means that the filter medium is arrange
  • the filter medium 15 is disposed on the entire inside of the introducing part 6, and in FIGS. 15 (a) and 15 (b), the filter medium 15 is one of the parts in the introducing part 6. It is arranged in the department.
  • the filter medium when the filter medium is disposed at a part in the introduction part, a space exists in the introduction part on the upstream side of the flow of the sample in the introduction part, but by introducing the sample into the introduction part Since the space can be filled with the sample, as described above, the component to be examined can be separated quickly and easily by the filter medium using capillary action by the flow path.
  • the introduction part be filled with a filter medium. That is, it is preferable that the filter medium be disposed in the entire introduction section. That is, it is preferable that the filter medium be disposed substantially without gaps in the introduction portion. Thereby, the component to be examined can be efficiently separated by the filter medium. In addition, due to the capillary phenomenon due to the flow path, it is possible to rapidly introduce the sample that has permeated the filter medium into the flow path. Furthermore, by filling the filter medium in the introduction part, the filter medium can be arranged to be in contact with the boundary between the introduction part and the flow path with certainty.
  • the filter medium may be any one as long as it can separate the component to be inspected and the component not to be inspected, and is appropriately selected according to the type of sample, the application of the inspection device, and the like.
  • the filter medium may be, for example, a filter medium for surface filtration or a filter medium for deep-layer filtration.
  • Surface filtration is a filtration mechanism that mainly captures specific components on the surface of the filter medium, and is also referred to as a screen filter.
  • depth filtration is a filtration mechanism that mainly captures specific components inside the filter medium, and is also referred to as a depth filter.
  • the filter medium is preferably a filter medium for deep bed filtration.
  • the depth filtration filter medium can retain a larger amount of particles than the surface filtration filter medium and is less likely to cause clogging.
  • deep filtration media are suitable.
  • the filter medium for deep bed filtration is suitable when the sample is blood.
  • the blood cell component is a relatively large particle having a particle diameter of, for example, 5 ⁇ m or more and 10 ⁇ m or less, and is contained in a large amount of about 45% in the blood.
  • a filter medium is suitably used.
  • the filter medium may be, for example, a fiber filter medium or a membrane filter.
  • fibrous filter media are preferably used.
  • the fibrous filter medium can hold a larger amount of particles than the membrane filter and is less likely to cause clogging. Therefore, fibrous filter media are suitable in the present disclosure that allow for filtration without external effects.
  • a membrane filter is often used as a surface filtration filter medium
  • a fiber filter medium is often used as a depth filtration filter medium.
  • fibers contained in the fibrous filter medium include cellulose fibers, glass fibers, nylon fibers, polyester fibers, acrylic fibers, polypropylene fibers, aramid fibers, animal fibers, chitosan fibers, carbon fibers, rock fibers, slug fibers, Metal fibers, composite fibers thereof and the like can be mentioned.
  • the fibers contained in the fibrous filter medium are preferably hydrophilic fibers. It is because the fiber filter medium containing hydrophilic fiber becomes easy to permeate
  • a hydrophilic fiber a cellulose fiber, glass fiber, an animal fiber etc. are mentioned, for example.
  • cellulose fiber and glass fiber are preferable because of high hydrophilicity.
  • glass fiber is preferred.
  • the filter medium containing glass fiber is a filter medium for deep layer filtration, and the glass fiber is a hydrophilic fiber and is excellent in separating plasma components and blood cell components in blood.
  • the membrane filter for example, polyether sulfone, nylon, polyester, polyethylene, polypropylene, polyimide, cellulose, cellulose acetate, cellulose mixed ester, polytetrafluoroethylene (PTFE), polyvinylidene fluoride (PVDF), etc. It can be mentioned.
  • the particle holding ability of the filter medium is sufficient as long as the component to be inspected and the component not to be inspected can be separated, and is appropriately selected according to the type of sample, the application of the inspection device, and the like.
  • the particle retention ability can be, for example, 0.1 ⁇ m or more, and preferably 0.5 ⁇ m or more.
  • the particle retention ability can be 10 ⁇ m or less, and preferably 5 ⁇ m or less. If the particle retention ability is within the above range, clogging can be less likely to occur.
  • the antigen is specifically a pathogen such as virus or bacteria, a protein, a hormone, an autoantibody, a nucleic acid, etc.
  • the size is, for example, about several nm to several tens of nm.
  • the particle retention ability may be larger than the size of the antigen, and can be in the above range.
  • the particle retention ability may be equal to or less than the size of the cellular components, and the size of the cellular components is about several tens ⁇ m to several hundreds ⁇ m if the size is large. The particle retention ability can be in the above range.
  • the particle retention ability can be 0.5 ⁇ m or more, and preferably 1 ⁇ m or more. In this case, the particle retention ability can be 5 ⁇ m or less, and preferably 2 ⁇ m or less. If the particle retention ability is within the above range, clogging can not occur and filtration can be performed rapidly.
  • the particle holding capacity may be larger than the size of the antigen, and can be in the above range.
  • the size of the blood cell component is, for example, 5 ⁇ m or more and 10 ⁇ m or less, and the particle retention ability may be equal to or less than the size of the blood cell component be able to.
  • the particle retention ability in the present disclosure is 7 kinds of test powder using 7 kinds of test powder 1 (7 kinds of test powder) defined in JIS Z 8901 (test powder and test particles).
  • This particle size is defined as a particle size capable of retaining 98% when natural dispersion water (hereinafter referred to as a sample) is naturally filtered.
  • the particle retention ability is measured as follows. First, the particle size distribution of the sample is measured according to JIS Z 8820-2 (Method of measuring particle size distribution by liquid phase gravity sedimentation method-Part 2) or JIS Z 8822 (Method of measuring particle size distribution of powder by sedimentation mass method) .
  • a 90 mm diameter filter medium is folded in four and attached to a funnel, into which 50 mL of a sample is poured, and the whole is naturally filtered. Finally, the particle size distribution of the collected filtrate is measured by the same method, and the particle diameter retained 98% by the filter medium is calculated.
  • the diameter of a filter medium and the volume of a sample can be arbitrarily selected in the range which does not affect a measurement result.
  • the thickness and the size of the filter medium are appropriately set according to the arrangement position of the filter medium, the size of the introduction part, and the like.
  • first base and second base are members for forming the flow path, the first base is disposed on the bottom side of the flow path, and the second base is It is disposed on the top side of the flow path.
  • the first substrate and the second substrate may be flexible or rigid. When at least one of the first substrate and the second substrate has rigidity, the handling of the inspection device is facilitated. In addition, when the first substrate and the second substrate have flexibility, the first substrate and the second substrate can be thinned to thin the inspection device of the present disclosure, and the cost can be reduced. .
  • the first substrate may be transparent or opaque.
  • the second substrate is preferably transparent.
  • the first substrate and the second substrate may be any ones that do not transmit liquid, and examples of the material include plastics, silicone rubber, glass, quartz and the like.
  • plastic include polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), polymethyl methacrylate resin (PMMA), acrylonitrile-butadiene-styrene copolymer (ABS) resin, Polyethylene terephthalate (PET), polycarbonate (PC), cyclic polyolefin (COP or COC), polyimide (PI), polyamide such as nylon, etc. may be mentioned.
  • silicone rubbers include polydimethylsiloxane (PDMS) and the like.
  • the surface on the flow path side of the first substrate has hydrophilicity.
  • the bottom surface of the flow path, the bottom surface of the liquid holding portion, and the bottom surface of the introduction portion can be made hydrophilic.
  • substrate has hydrophilicity.
  • the top surface of the flow path can be made hydrophilic.
  • the hydrophilicity can be the same as the hydrophilicity described in the section of the flow path.
  • a material having hydrophilicity may be used for the first substrate and the second substrate, and the first substrate
  • the surface on the flow channel side of the second substrate and the surface on the flow channel side of the second base may be subjected to a hydrophilization treatment.
  • a general method can be applied as the method of hydrophilization treatment.
  • surface treatment methods such as plasma treatment, glow treatment, corona treatment, UV ozone treatment, methods using surface treatment agents such as surfactants and silane coupling agents, methods of depositing silica, hydrophilic polymers And surface treatment.
  • At least one of the first base and the second base may have a groove serving as a flow path, a liquid holding portion, an introducing portion, and the like.
  • the method for forming the groove is not particularly limited, and examples thereof include cutting, injection molding, photolithography, laser ablation, and hot embossing.
  • the thickness of the first substrate and the second substrate is appropriately set according to the application of the inspection device of the present disclosure, but may be, for example, 0.01 mm or more, and may be 0.05 mm or more. It may be 0.2 mm or more, or 0.5 mm or more. Moreover, the said thickness can be 2 mm or less, may be 1 mm or less, may be 0.5 mm or less, and may be 0.25 mm or less.
  • the size and the like of the first substrate and the second substrate are appropriately set according to the application of the inspection device of the present disclosure.
  • the inspection device of the present disclosure can have a spacer between the first substrate and the second substrate.
  • the spacer is a member for providing a space between the first base and the second base, and for forming a flow path, an introducing portion, and the like.
  • an adhesive layer for example, an adhesive layer, a resin layer, etc. can be used.
  • the adhesive layer general adhesives and adhesives can be used. Further, as the adhesive layer, for example, a double-sided tape may be used. For example, a resin base may be used as the resin layer.
  • the thickness of the spacer may be similar to the height of the flow path.
  • the planar view shape of the spacer is a shape having an opening portion which becomes a flow path, a liquid holding portion, an introducing portion and the like.
  • the method of arranging the spacer is appropriately selected according to the material of the spacer and the like.
  • a double-sided tape as an adhesive layer
  • the method of sticking a double-sided tape on the surface of is mentioned.
  • a resin layer is used as a spacer, an opening serving as a flow path, a liquid holding portion, an introducing portion, etc. is formed in a resin base material by punching or the like, and then one side of the first base or the second base
  • the test device of the present disclosure can have a reagent carried by the filter medium.
  • the reagent carried on the filter medium is appropriately selected according to the type of sample and the application of the test device, and is labeled with a labeling substance such as an enzyme, a chemiluminescent substance, a fluorescent substance, a metal colloid, a metal complex, etc. Labeled antibodies can be mentioned.
  • the enzyme is not particularly limited, and specific examples include horseradish peroxidase (HRP), alkaline phosphatase (ALP), ⁇ -D-galactosidase, dehydrogenase and the like.
  • the chemiluminescent substance is not particularly limited. Specifically, N-methyl acridinium ester derivative, N-methyl acridinium acyl sulfonamide ester derivative, N- (4-aminobutyl) -N-ethyl iso A luminol ester derivative etc. are mentioned.
  • the fluorescent substance is not particularly limited, and specific examples include europium, samarium, terbium, dysprosium, fluorescent silica nanoparticles and the like.
  • the metal colloid is not particularly limited, and specific examples thereof include gold colloid, silver colloid, selenium colloid, palladium colloid, platinum colloid and the like.
  • the metal complex is not particularly limited, and specific examples thereof include a ruthenium complex and luminol.
  • the inspection device of the present disclosure can also have a reagent immobilized in the flow channel.
  • the reagent to be immobilized in the channel is appropriately selected according to the type of sample and the application of the test device, and examples include proteins, antibodies, enzymes, nucleic acids and the like.
  • the reagent immobilized in the flow channel is preferably an antibody that binds to the antigen contained in the sample.
  • the reagent immobilized in the flow channel may be an antibody which binds to the above-described labeled antibody carried on the filter medium.
  • the reagents may be used alone or in combination of two or more.
  • the reagent may be fixed in the channel, for example, may be carried on the surface of the first substrate on the channel side, or the carrier may be disposed in the channel and carried on the carrier. .
  • a general method can be applied as a method of loading the reagent on the surface on the flow path side of the first substrate.
  • the method of loading the reagent on the carrier is not particularly limited, and examples thereof include methods such as covalent bonding, ionic bonding, hydrophobic interaction, and biological interaction. Among them, it is preferable that the reagent be immobilized on a carrier via a covalent bond. This is because covalent immobilization can suppress the detachment of the immobilized reagent, ie, the immobilized antibody from the carrier.
  • the carrier for carrying the reagent is not particularly limited, and the carrier material may be, for example, an inorganic material or an organic material.
  • the inorganic material include glass, silica, quartz, alumina, talc, clay, mica, aluminum, iron, titanium oxide, zinc oxide, iron oxide, graphite, carbon black, calcium carbonate and the like.
  • organic material examples include polyethylene (PE), polypropylene (PP), polystyrene (PS), polyvinyl chloride (PVC), polymethyl methacrylate resin (PMMA), acrylonitrile-butadiene-styrene copolymer (ABS) resin And polyethylene terephthalate (PET), polycarbonate (PC), cyclic polyolefin (COP or COC), polyimide (PI), and polyamides such as nylon.
  • PE polyethylene
  • PP polypropylene
  • PS polystyrene
  • PVC polyvinyl chloride
  • PMMA polymethyl methacrylate resin
  • ABS acrylonitrile-butadiene-styrene copolymer
  • PET polyethylene terephthalate
  • PC polycarbonate
  • COC cyclic polyolefin
  • PI polyimide
  • polyamides such as nylon.
  • the carrier examples include flat plates, disks, particles and the like. Among them, particles are preferred because of their large surface area.
  • the carrier may have fine asperities on its surface.
  • the thickness of the carrier is not particularly limited as long as it is equal to or less than the height of the flow channel.
  • the flow path can have one or more reagent placement parts on which reagents are fixed, and when the flow path has a plurality of reagent placement parts, the reagent to be fixed to each reagent placement part is And may be appropriately selected depending on the application of the inspection device.
  • the reagents immobilized on the respective reagent placement parts may be different from one another.
  • the labeled antibody carried by the filter medium is preferably released into the sample by dropping the sample.
  • the released labeled antibody flows in the channel toward the absorber while binding to the antigen contained in the sample. Furthermore, it binds to an antibody immobilized in the flow path (hereinafter referred to as immobilized antibody) to form a sandwich immunocomplex consisting of immobilized antibody-antigen-labeled antibody.
  • This immune complex can be detected by optical means such as luminescence, fluorescence, absorbance, light scattering and the like.
  • the inspection device of the present disclosure can have an absorber fixing unit 16 that fixes the absorber 10, as shown in FIGS. 16 and 17, for example.
  • the absorber fixing portion fixes the absorber and can prevent the absorber from falling off from the liquid holding portion.
  • 16 and 17 are a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure.
  • FIG. 16B is a cross-sectional view taken along the line AA of FIG. 17 (b) is a cross-sectional view taken along the line AA of FIG. 17 (a)
  • FIG. 17 (c) is a cross-sectional view taken along the line BB of FIG. 17 (c).
  • the fixed part for absorbers As an arrangement position of the fixed part for absorbers, the fixed part for absorbers is arranged so that an air port can be arranged between a channel and an absorber, and an absorber can be fixed. It should just be suitably selected according to the arrangement position of the above-mentioned air opening, the arrangement position of the above-mentioned absorber, etc.
  • the absorber fixing portion is usually disposed on the top of the absorber.
  • an adhesive tape can be used.
  • the shape and area of the fixing portion for absorber can be appropriately set according to the shape, area, and the like of the liquid holding portion and the absorber.
  • the arrangement position, shape, area, etc. of the air port can be adjusted by adjusting the arrangement position, shape, area, etc. of the absorber fixing portion.
  • the inspection device of the present disclosure can have a filter medium fixing section 17 that fixes the filter medium 15, as shown in FIGS. 16 and 17, for example.
  • the filter medium is fixed by the filter medium fixing portion, and the filter medium can be prevented from falling off from the introduction portion.
  • the arrangement position of the filter medium fixing portion is appropriately selected according to the arrangement position of the filter medium, the arrangement position of the introduction port of the introduction unit, and the like.
  • the filter medium fixing part is usually disposed on the surface of the filter medium on the introduction port side.
  • the filter medium fixing part is usually disposed on the upper part of the filter medium.
  • the fixing part for filter media is normally arrange
  • the filter medium fixing portion may be disposed on the entire circumference of the filter medium in a plane parallel to the surface on the inlet side of the introduction unit, or may be disposed on a part of the periphery of the filter medium.
  • the filter medium fixing part is usually a plane parallel to the plane on the inlet side of the introducing part.
  • an opening that overlaps the filter medium When the filter medium fixing portion has the opening, the filter medium is exposed at the opening, and the sample can be introduced from the opening.
  • the surface on the opposite side to the surface at the side of the filter medium of the fixing part for filter media has hydrophobicity. This is because the liquid introduced from the introduction port can be held inside the opening of the filter medium fixing portion.
  • hydrophobic means that the static contact angle of water on a predetermined surface is more than 60 degrees.
  • the static contact angle of water on a given surface is preferably 90 degrees or more.
  • the static contact angle of water on a given surface is typically 150 degrees or less.
  • the filter medium fixing portion is not particularly limited as long as the filter medium can be fixed, and for example, an adhesive tape can be used.
  • the shape and area of the filter medium fixing portion can be appropriately set in accordance with the shape, area, and the like of the introduction portion.
  • the shape and the area of the opening can be appropriately set according to the amount of the sample.
  • Housing The inspection device of the present disclosure can have a housing that houses the first base and the second base.
  • the housing protects the first base and the second base, and can suppress deformation of the first base and the second base. In addition, since deformation of the first base and the second base is suppressed, the inspection can be performed with high accuracy.
  • the housing may be transparent or opaque.
  • the housing usually has rigidity.
  • the housing usually has an opening that overlaps the inlet in a plane parallel to the surface on the inlet side of the inlet. At this opening, the filter medium is exposed, and the liquid can be introduced from this opening.
  • the housing can have other openings in plan view.
  • the housing can have an opening that overlaps the reagent placement portion in plan view.
  • FIG. 18 is a schematic plan view and a cross-sectional view showing another example of the inspection device of the present disclosure
  • FIG. 18 (b) is a cross-sectional view taken along line CC of FIG. 18 (a).
  • the inspection device 1 can have a housing 35 that accommodates the first base 2 and the second base 3.
  • the housing 35 can have an opening 36 overlapping the inlet 5 in plan view.
  • a sample can be introduced from the opening 36.
  • the housing 35 can have the openings 37 and 38 overlapping the reagent placement units 21S and 22S in plan view, respectively.
  • the plan view shape of the opening of the housing is not particularly limited, and examples thereof include a circle, an ellipse, a triangle, and a rectangle.
  • the area of the opening in a plan view can be set arbitrarily.
  • the housing is not particularly limited as long as it can protect the first base and the second base and can suppress deformation of the first base and the second base.
  • Examples of the material include Plastic is mentioned.
  • injection molding can be used as a method for manufacturing the housing.
  • the size of the housing is appropriately selected according to the size of the first base and the second base, the use of the inspection device, and the like.
  • Liquids applied to the test devices of the present disclosure include analytes.
  • the sample is preferably a sample used for an immunological test. Specifically, blood (whole blood), plasma, serum, nasal swab, pharyngeal swab, gargle, nasal discharge, urine, saliva, tears and the like can be mentioned. These samples may be diluted as needed.
  • liquid other than the sample is appropriately selected according to the application of the inspection device and the like.
  • washing solution antibody solution, substrate solution and the like can be mentioned.
  • FIG. 19 and FIG. 20 are process drawings showing an example of the method of manufacturing the inspection device of the present disclosure, showing a plan view and a sectional view taken along the line DD of the plan view.
  • the first base 2 and a spacer 12 having a predetermined opening are bonded.
  • the reagents 21 and 22 are fixed to the surface of the first base 2 on the spacer 12 side.
  • the reagent 21 disposed on the inlet side can be an antibody that binds to the antigen contained in the sample.
  • the reagent 22 disposed on the liquid holding unit side can be an antibody that binds to the labeled antibody carried on the filter medium.
  • the second base 3 is bonded to the surface of the spacer 12 opposite to the surface on the first base 2 side. Thereby, the flow path 4 is formed.
  • the second base 3 is processed to form an opening, and the inlet 5, the inlet 6 and the liquid holder 7 are formed.
  • the second base 2 may be bonded to the spacer 12 before forming the opening, or may be bonded to the spacer 12 after forming the opening.
  • FIG. 19C the second base 3 is bonded to the surface of the spacer 12 opposite to the surface on the first base 2 side. Thereby, the flow path 4 is formed.
  • the second base 3 is processed to form an opening, and the inlet 5, the inlet 6 and the liquid holder 7 are formed.
  • the second base 2 may be bonded to the spacer 12 before forming the opening, or may be bonded to the spacer 12 after forming the opening.
  • the first absorber 8 is disposed from the inside of the liquid holding unit 7 to the inside of the flow path 4.
  • the second absorber 9 is disposed only in the liquid holding unit 7, and the second absorber 9 is stacked on the first absorber 8.
  • the filter medium 15 is disposed in the introduction portion 6.
  • the absorber fixing portion 16 is disposed on the top of the absorber 10.
  • the filter medium fixing portion 17 is disposed on the top of the filter medium 15.
  • the inspection device of the present disclosure can be used for various inspections.
  • the test device of the present disclosure can be applied to a test that utilizes an antigen-antibody reaction or an enzyme reaction.
  • an optical method may be used or an electrochemical method may be used.
  • the inspection device of the present disclosure can have an electrode in the flow path.
  • the inspection device of the present disclosure can perform inspection alone, for example, or can be used as a replaceable cartridge.
  • the present disclosure is not limited to the above embodiment.
  • the above-described embodiment is an exemplification, which has substantially the same configuration as the technical idea described in the claims of the present disclosure, and exhibits the same operation and effect as the present invention. It is included in the technical scope of the disclosure.
  • Example 1 (Production of inspection device) First, a PET film (thickness: 0.1 mm) hydrophilized by corona treatment was prepared. At this time, the static contact angle of water on the hydrophilized surface of the PET film was about 30 °. Next, a double-sided tape (core: PET, thickness: 0.1 mm) was cut out in the same shape as the spacer in FIG. 19 (a). One side of this double-sided tape was attached to the hydrophilized side of the PET film. Subsequently, as shown in FIG.
  • a glass bead (0.1 mm thick) having a monoclonal antibody that binds to bovine serum albumin (BSA) immobilized on the exposed portion of the PET film is supported on a filter medium Glass beads (diameter 0.1 mm) immobilized with an antibody that binds to HRP-labeled monoclonal antibody were placed from the left side.
  • the PET film (thickness: 0.1 mm) similarly hydrophilized by corona treatment was affixed on the other side of a double-sided tape. Thereafter, as shown in FIG. 19 (d), an opening serving as an introducing portion and a liquid holding portion was formed in the upper PET film using a cutting plotter.
  • a tracing paper (thickness: 0.06 mm) cut into the shape of FIG. 2 (c) from the inside of the liquid holding portion to the inside of the flow path is disposed as the first absorber. did.
  • the creme water absorption of the tracing paper was less than 5 mm.
  • a cellulose filter paper (thickness: 0.2 mm) was disposed in the liquid holding unit as a second absorbent body, and the cellulose filter paper was laminated on the tracing paper.
  • cellulose filter paper (thickness: 0.2 m) carrying HRP-labeled monoclonal antibody was filled in the introduction part.
  • the Klem water absorption of these cellulose filter papers was 75 mm.
  • an adhesive tape (thickness: 0.03 mm) was attached to the top of the liquid holding portion and the introduction portion.
  • the dimensions of the obtained inspection device are 26 mm ⁇ 76 mm, the dimensions of the liquid holder are 20 mm ⁇ 20 mm, the dimensions of the exposed portion of the filter medium are 10 mm ⁇ 5 mm, the total length of the flow path is 40 mm, and the width of the flow path is 0. It was 5 mm.
  • ELISA method was implemented as follows using the obtained test device. First, 20 ⁇ L of a phosphate buffer solution (PBS) (hereinafter, sample) containing 10 ng / mL of bovine serum albumin (BSA) was dropped from the inlet into the inlet. The sample passed through the cellulose filter within a few seconds and penetrated into the flow path. After about 30 seconds, the sample reached the glass beads, and 30 seconds later, the protrusions of the tracing paper were reached. Soon, the sample was absorbed by the tracing paper in the liquid holding unit, and several seconds later, it was also absorbed by the cellulose filter paper. After about 20 seconds, when there was no sample in the introduction part, the sample was separated at the interface with the tracing paper in the flow path.
  • PBS phosphate buffer solution
  • BSA bovine serum albumin
  • washing solution phosphate buffer solution (PBS) containing 0.05% Tween (surfactant)
  • PBS phosphate buffer solution
  • surfactant surfactant
  • TMB 3,3 ′, 5,5′-tetramethylbenzidine
  • test device of the present disclosure can rapidly carry out a highly sensitive ELISA method with a simple operation.
  • Example 2 (Production of inspection device) As shown in Table 1 below, in the same manner as Example 1, except that the width of the flow path and the length of the protrusion (square shape) of the tracing paper which is the first absorber were variously changed , The inspection device was produced. The width of the protrusion was the same as the width of the flow channel.

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Abstract

La présente invention concerne un dispositif d'inspection comprenant : un premier corps de base ; un second corps de base faisant face au premier corps de base ; un trajet d'écoulement situé entre le premier corps de base et le second corps de base ; un orifice d'introduction relié à une extrémité du trajet d'écoulement et introduisant un liquide ; une partie maintien de liquide reliée à l'autre extrémité du trajet d'écoulement et possédant un corps d'absorption disposé au niveau de cette dernière ; un corps d'absorption disposé à l'intérieur de la partie maintien de liquide ; et un orifice d'air disposé entre le trajet d'écoulement et le corps d'absorption et se raccordant au trajet d'écoulement.
PCT/JP2018/047515 2017-12-25 2018-12-25 Dispositif d'inspection WO2019131606A1 (fr)

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CN117063072A (zh) * 2021-01-08 2023-11-14 日本火腿株式会社 测定装置
WO2023085006A1 (fr) * 2021-11-15 2023-05-19 国立研究開発法人産業技術総合研究所 Dispositif de dosage électrochimique
JP2023125105A (ja) * 2022-02-28 2023-09-07 藤森工業株式会社 液体試料分析用マイクロチップおよびその製造方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007139781A (ja) * 2005-11-21 2007-06-07 Lifescan Inc バイオセンサー装置及びその使用方法
JP2008514966A (ja) * 2004-09-30 2008-05-08 クイデル コーポレイション 第１及び第２流路を有する分析装置
JP2009031102A (ja) * 2007-07-26 2009-02-12 Panasonic Corp 試料分析チップ
JP2010054308A (ja) * 2008-08-27 2010-03-11 Sharp Corp 検出器具、分析装置および検出方法
WO2013147217A1 (fr) * 2012-03-30 2013-10-03 国立大学法人九州大学 Capteur, procédé de détection, système de détection et dispositif de détection
WO2014051033A1 (fr) * 2012-09-28 2014-04-03 独立行政法人産業技術総合研究所 Dispositif de dosage utilisant un milieu poreux

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008514966A (ja) * 2004-09-30 2008-05-08 クイデル コーポレイション 第１及び第２流路を有する分析装置
JP2007139781A (ja) * 2005-11-21 2007-06-07 Lifescan Inc バイオセンサー装置及びその使用方法
JP2009031102A (ja) * 2007-07-26 2009-02-12 Panasonic Corp 試料分析チップ
JP2010054308A (ja) * 2008-08-27 2010-03-11 Sharp Corp 検出器具、分析装置および検出方法
WO2013147217A1 (fr) * 2012-03-30 2013-10-03 国立大学法人九州大学 Capteur, procédé de détection, système de détection et dispositif de détection
WO2014051033A1 (fr) * 2012-09-28 2014-04-03 独立行政法人産業技術総合研究所 Dispositif de dosage utilisant un milieu poreux

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