WO2019130018A1 - Procédés d'augmentation du rendement et/ou de la tolérance au stress abiotique - Google Patents

Procédés d'augmentation du rendement et/ou de la tolérance au stress abiotique Download PDF

Info

Publication number
WO2019130018A1
WO2019130018A1 PCT/GB2018/053702 GB2018053702W WO2019130018A1 WO 2019130018 A1 WO2019130018 A1 WO 2019130018A1 GB 2018053702 W GB2018053702 W GB 2018053702W WO 2019130018 A1 WO2019130018 A1 WO 2019130018A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
nucleic acid
sequence
seq
mutation
Prior art date
Application number
PCT/GB2018/053702
Other languages
English (en)
Inventor
Hongning Tong
Chengcai Chu
Wenchao YIN
Yunhua XIAO
Dapu LIU
Guoxia ZHANG
Original Assignee
Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
Williams, Andrea
Institute Of Crop Science, Chinese Academy Of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences, Williams, Andrea, Institute Of Crop Science, Chinese Academy Of Agricultural Sciences filed Critical Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
Publication of WO2019130018A1 publication Critical patent/WO2019130018A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

L'invention concerne des procédés d'augmentation du rendement et/ou de la tolérance au stress abiotique dans une plante, des plantes transgéniques présentant un rendement accru et/ou une tolérance au stress abiotique et des procédés de production de telles plantes. En particulier, l'invention comprend l'augmentation de l'expression ou de l'activité de AGO2, notamment à un niveau modéré.
PCT/GB2018/053702 2017-12-25 2018-12-19 Procédés d'augmentation du rendement et/ou de la tolérance au stress abiotique WO2019130018A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201711423192.7A CN107868123B (zh) 2017-12-25 2017-12-25 一种同时提高植物产量和抗性的基因及其应用
CN201711423192.7 2017-12-25

Publications (1)

Publication Number Publication Date
WO2019130018A1 true WO2019130018A1 (fr) 2019-07-04

Family

ID=61756100

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2018/053702 WO2019130018A1 (fr) 2017-12-25 2018-12-19 Procédés d'augmentation du rendement et/ou de la tolérance au stress abiotique

Country Status (2)

Country Link
CN (1) CN107868123B (fr)
WO (1) WO2019130018A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110713527A (zh) * 2018-07-11 2020-01-21 中国农业大学 Bin2及其编码基因在调控植物耐盐中的应用
CN109777792B (zh) * 2018-09-29 2020-12-11 中国科学院动物研究所 一种rna解旋酶3及其编码基因和应用
CN112812162B (zh) * 2021-02-08 2022-07-19 江苏省农业科学院 一种水稻抗性相关基因及其应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565350A (en) 1993-12-09 1996-10-15 Thomas Jefferson University Compounds and methods for site directed mutations in eukaryotic cells
WO2000015815A1 (fr) 1998-09-14 2000-03-23 Pioneer Hi-Bred International, Inc. Genes de type rac de mais et methodes d'utilisations
US20040123343A1 (en) * 2000-04-19 2004-06-24 La Rosa Thomas J. Rice nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
WO2011061656A1 (fr) * 2009-11-17 2011-05-26 Basf Plant Science Company Gmbh Plantes à rendement accru
US8440431B2 (en) 2009-12-10 2013-05-14 Regents Of The University Of Minnesota TAL effector-mediated DNA modification
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565350A (en) 1993-12-09 1996-10-15 Thomas Jefferson University Compounds and methods for site directed mutations in eukaryotic cells
WO2000015815A1 (fr) 1998-09-14 2000-03-23 Pioneer Hi-Bred International, Inc. Genes de type rac de mais et methodes d'utilisations
US20040123343A1 (en) * 2000-04-19 2004-06-24 La Rosa Thomas J. Rice nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
WO2011061656A1 (fr) * 2009-11-17 2011-05-26 Basf Plant Science Company Gmbh Plantes à rendement accru
US8440431B2 (en) 2009-12-10 2013-05-14 Regents Of The University Of Minnesota TAL effector-mediated DNA modification
US8440432B2 (en) 2009-12-10 2013-05-14 Regents Of The University Of Minnesota Tal effector-mediated DNA modification
US8450471B2 (en) 2009-12-10 2013-05-28 Regents Of The University Of Minnesota TAL effector-mediated DNA modification
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ALLISON MALLORY ET AL: "Form, Function, and Regulation of ARGONAUTE Proteins", THE PLANT CELL, vol. 22, no. 12, 1 December 2010 (2010-12-01), US, pages 3879 - 3889, XP055563176, ISSN: 1040-4651, DOI: 10.1105/tpc.110.080671 *
CERMAK T ET AL.: "Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting", NUCLEIC ACID RESEARCH, vol. 39, 2011, XP055130093, DOI: doi:10.1093/nar/gkr218
HIEI Y; OHTA S; KOMARI T; KUMASHIRO T: "Efficient transformationof rice(Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the", T-DNA. PLANT J, vol. 6, 1994, pages 271 - 82
KAPOOR MEENU ET AL: "Genome-wide identification, organization and phylogenetic analysis of Dicer-like, Argonaute and RNA-dependent RNA Polymerase gene families and their expression analysis during reproductive development and stress in rice", BMC GENOMICS, BIOMED CENTRAL, vol. 9, no. 1, 1 October 2008 (2008-10-01), pages 451, XP021042189, ISSN: 1471-2164, DOI: 10.1186/1471-2164-9-451 *
MÁRTA LUDMAN ET AL: "Crispr/Cas9 Mediated Inactivation of Argonaute 2 Reveals its Differential Involvement in Antiviral Responses", SCIENTIFIC REPORTS, vol. 7, no. 1, 21 April 2017 (2017-04-21), XP055563183, DOI: 10.1038/s41598-017-01050-6 *
SAMBROOK ET AL.: "Molecular Cloning: A Library Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
XIAO Y; LIU D; ZHANG G; TONG H; CHU C: "Brassinosteroids Regulate OFP1, a DLT Interacting Protein, to Modulate Plant Architecture and Grain Morphology in Rice", FRONT. PLANT SCI, 2017
XIAO Y; LIU D; ZHANG G; TONG H; CHU C: "Brassinosteroids Regulate OFP1, a DLT Interacting Protein, to Modulate Plant Architecture and Grain Morphologyin Rice", FRONT. PLANT SCI., 2017

Also Published As

Publication number Publication date
CN107868123A (zh) 2018-04-03
CN107868123B (zh) 2020-05-12

Similar Documents

Publication Publication Date Title
US9809827B2 (en) Transgenic maize
US11873499B2 (en) Methods of increasing nutrient use efficiency
US10982225B2 (en) Flowering time-regulating genes and related constructs and applications thereof
US20190085355A1 (en) Drought tolerant maize
US10988775B2 (en) Wheat plants resistant to powdery mildew
WO2017107983A1 (fr) Procédé permettant d'augmenter l'efficacité d'utilisation d'azote chez des végétaux
CN111433363B (zh) 非生物胁迫耐性提高的植物和提高植物非生物胁迫耐性的多聚核苷酸及方法
CN111718914B (zh) 蛋白ZmTIP1在调控植物抗旱性中的应用
CN115927380A (zh) 具有增强性状的转基因植物
US20220396804A1 (en) Methods of improving seed size and quality
WO2019130018A1 (fr) Procédés d'augmentation du rendement et/ou de la tolérance au stress abiotique
WO2014069339A1 (fr) Acide nucléique conférant une propriété de rendement élevé à une plante, procédé pour produire une plante transgénique à fort rendement et procédé pour accroître le rendement de la plante.
CN111295445B (zh) 非生物胁迫耐性提高的植物和提高植物非生物胁迫耐性的多聚核苷酸及方法
WO2015007241A1 (fr) Marqueur moléculaire
CN111154767B (zh) 根长调控基因logl5及相应的构建体和其应用
US20180105824A1 (en) Modulation of dreb gene expression to increase maize yield and other related traits
CN111826391A (zh) 一种nhx2-gcd1双基因或其蛋白的应用
US20080189805A1 (en) Novel genes and rna molecules that confer stress tolerance
CN112204144B (zh) 非生物胁迫耐受植物和使用方法
CN110959043A (zh) 利用bcs1l基因和向导rna/cas核酸内切酶系统改良植物农艺性状的方法
US20080189804A1 (en) Novel genes and rna molecules that confer stress tolerance
CN112041448B (zh) 氮素限制条件下农艺性状改变的植物和非生物胁迫耐性基因相关的构建体和方法
WO2023152164A1 (fr) Canaux ioniques modifiés
CN115044592A (zh) 一种调控玉米株型和瘤黑粉病抗性的基因ZmADT2及其编码蛋白和应用
CN116789785A (zh) 长雄蕊野生稻高产、高光效基因FarL1及其应用

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18830504

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18830504

Country of ref document: EP

Kind code of ref document: A1

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 24/11/2020)

122 Ep: pct application non-entry in european phase

Ref document number: 18830504

Country of ref document: EP

Kind code of ref document: A1