Classifications

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  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
  • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
  • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
  • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
  • C07K16/3076—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
  • C07K16/3084—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated gangliosides
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
  • A61K31/19—Carboxylic acids, e.g. valproic acid
  • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
  • A61K31/203—Retinoic acids ; Salts thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K38/00—Medicinal preparations containing peptides
  • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • A61K38/19—Cytokines; Lymphokines; Interferons
  • A61K38/193—Colony stimulating factors [CSF]
• • A—HUMAN NECESSITIES
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  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K39/00—Medicinal preparations containing antigens or antibodies
  • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
  • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
  • A61K39/39558—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against tumor tissues, cells, antigens
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K39/00—Medicinal preparations containing antigens or antibodies
  • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
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  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
  • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
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  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
  • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
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  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/52—Constant or Fc region; Isotype
  • C07K2317/522—CH1 domain
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  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/52—Constant or Fc region; Isotype
  • C07K2317/524—CH2 domain
• • C—CHEMISTRY; METALLURGY
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  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/52—Constant or Fc region; Isotype
  • C07K2317/526—CH3 domain
• • C—CHEMISTRY; METALLURGY
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  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/52—Constant or Fc region; Isotype
  • C07K2317/53—Hinge
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  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
  • C07K2317/565—Complementarity determining region [CDR]
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  • C07—ORGANIC CHEMISTRY
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  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
  • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
  • C07K2317/732—Antibody-dependent cellular cytotoxicity [ADCC]
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  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
  • C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
  • C07K2317/734—Complement-dependent cytotoxicity [CDC]
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  • C07K2319/00—Fusion polypeptide
  • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
  • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor

Definitions

• the invention relates to the field of antibodies.
• it relates to antibodies that bind the ganglioside GD2.
• It further relates to the use of GD2 antibodies in medical and detection methods.
• the invention further relates to cells, nucleic acid molecules and methods for the production of the antibodies.
• Neuroblastoma the most common extracranial solid tumor of childhood. The majority of patients is diagnosed with high-risk neuroblastoma with a mortality of 50%. Neuroblastoma therapy consists of intensive multimodality treatment with severe toxicities. High-risk neuroblastoma therapy consists of intensive
• the antibody dinutuximab (trade name Unituxin), directed against ganglioside GD2, a carbohydrate antigen uniformly expressed on neuroblastoma and neural tissue, was FDA-approved for neuroblastoma treatment.
• Application of this antibody, combined with cytokines and differentiation factors, has improved patient prognosis and demonstrated that neuroblastoma is susceptible to immunotherapy (Yu et al., 2010; New Engl. J. Med, Vol 363: pp 1324-34; and Suzuki and Cheung., 2015; Expert Opin Ther Targets Vol 19: p. 349-62).
• Dinutuximab significantly improved event-free survival in comparison to standard treatment.
• Dinutuximab is a chimeric monoclonal antibody composed of the variable heavy- and light-chain regions of the murine anti-GD2 antibody 14.18 and the constant regions of human IgGl heavy-chain and kappa light-chain (Gillies et al., 1989; J Immunol Methods, 125: p. 191-202). It is directed against the end-terminal penta-oligosaccharide of GD2, an extracellularly expressed disialoganglioside on tissues of the central nervous system and peripheral nerves, as well as on many tumors of neuroectodermal origin, including neuroblastoma (Suzuki and Cheung, 2015; Expert Opin Ther Targets Vol 19: p. 349-62).
• Antibody produced in SP2/0 mouse myeloma cells can result in aberrant glycosylation with respect to natural human antibodies.
• the antibody is now produced in CHO cells.
• the uniform expression of GD2 on neuroblastoma together with the low expression on other tissues makes this tumor associated antigen a promising target for antibody therapy.
• Dinutuximab is used in combination with isotretinoin and alternating administration of IL-2 and GM-CSF for the first-line treatment against high-risk neuroblastoma in patients where a response after induction therapy was shown (Suzuki and Cheung, 2015; Expert Opin Ther Targets Vol 19: p. 349-62).
• ADCC antibody dependent cell mediated cytotoxicity
• CDC complement mediated cytotoxicity
• NK cells NK cells and possibly macrophages for their action.
• ADCC activity of dinutuximab against neuroblastoma is for some reason also dependent on granulocyte activation. This is shown in vitro and in vivo, by showing that patient outcome depends in part on granulocyte activation (Barker et al., 1991; Cancer Res, Vol 51: p.
• the anti-GD2 antibodies and the methods of treatment of present invention exhibit improved efficacy.
• toxicity associated with dinutuximab treatment is reduced, in particular pain associated toxicity is abrogated, as shown in pre-clinical models.
• the invention provides an anti-ganglioside GD2 antibody that comprises an antibody variable domain and antibody constant domains, wherein the variable domain comprises a heavy and light chain variable region comprising respectively at least the CDR3 of the heavy chain variable region of antibody chl4.18 and at least the CDR3 of the light chain variable region of antibody chl4.18; and an IgA hinge and CH2 domain.
• the variable domain preferably comprises a heavy and light chain variable region comprising respectively at least the CDR1, CDR2 and CDRl, CDR2 and CDR3 of the light chain variable region of antibody chl4.18.
• the variable domain comprises the heavy and light chain variable region of antibody chl4.18.
• the invention further provides a method of treatment of a subject that has a
• GD2 positive tumor or is at risk of having a GD2 positive tumor the method comprising administering a therapeutic amount of an antibody of the invention to the subject in need thereof.
• the method preferably further comprises administering retinoic acid in an amount effective to upregulate the ganglioside GD2 in said GD2 positive tumor, preferably neuroblastoma in said subject.
• the method may further comprise administering granulocyte-macrophage colony-stimulating factor (GM- CSF, granulocyte colony-stimulating factor (G-CSF) or a combination thereof to said subject.
• GM-CSF, G-CSF or a combination thereof can increase the number of granulocytes in the subject but is also administered to improve the induced cell killing.
• the invention further provides an antibody of the invention for use in the treatment of a subject that has a GD2 positive tumor or is at risk of having a GD2 positive tumor.
• the invention further provides an antibody of the invention in combination with retinoic acid for use in the treatment of a subject that has a GD2 positive tumor or is at risk of having a GD2 positive tumor.
• the invention further provides an antibody of the invention in combination with GM-CSF, G-CSF or a combination thereof for use in the treatment of a subject that has a GD2 positive tumor or is at risk of having a GD2 positive tumor.
• the invention further provides an antibody of the invention in combination with GM-CSF, G-CSF or a combination thereof and retinoic acid for use in the treatment of a subject that has a GD2 positive tumor or is at risk of having a GD2 positive tumor.
• the GD2 positive tumor is preferably a GD2 positive neuroblastoma such as a neuroectoderm-derived tumor or a sarcoma.
• the GD2 positive tumor is a GD2 positive neuroblastoma, retinoblastoma, melanoma, small cell lung cancer, brain tumor such as glioblastoma, osteosarcoma,
• the GD2 positive tumor is a neuroblastoma.
• neuroblastoma treated with a method or use of the invention is preferably a high risk neuroblastoma.
• a heavy chain comprising an IgA hinge and Cn2 domain.
• an engineered antibody or fragment thereof for use in improvement of patient compliance and/or a reduction of a side effect associated with immunoglobulin G (IgG) antibody therapy, wherein the engineered antibody or fragment thereof comprises: (a) CDR1, CDR2, and CDR3 of an immunoglobulin G (IgG); and (b) the constant regions or portion thereof of an immunoglobulin A (IgA), wherein the engineered antibody or fragment thereof results in reduction of at least one side effect upon administration to the subject as compared to
• the engineered antibody or fragment thereof further comprises a heavy chain and light chain variable region of IgG.
• the constant regions or portion thereof of the IgA comprises a hinge, a CH2 constant region, or a combination thereof.
• the constant regions or portion thereof comprises a hinge, a CH2 constant region, or a combination thereof.
• the constant regions comprise a constant heavy and constant light chain of the IgA.
• the constant regions or portion thereof of the IgA comprises a hinge and a constant heavy chain domain.
• the antibody or fragment thereof is chimerized, humanized, human, or non-human.
• the constant regions or portion thereof is human.
• the side effect comprises an innate immune response.
• the innate immune response comprises a complement response.
• the complement response comprises IgG binding to FcyRs or Clq.
• the side effect comprises one or more of pain, visceral
• the pain is acute pain, migraine or intense visceral pain.
• determining the reduction of the side effect is measured by performing flow cytometry, an in vitro assay, or a combination thereof.
• the flow cytometry comprises determining cellular lysis, binding, cell death, receptor expression, or a combination thereof.
• the flow cytometry comprises determining live/dead staining of a cell.
• the in vitro assay comprises ELISA, antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), hemolytic assay, or a combination thereof.
• the engineered antibody or fragment thereof when determining the reduction of the side effect is measured by performing flow cytometry, an in vitro assay, or a combination thereof.
• the flow cytometry comprises determining cellular lysis, binding, cell death, receptor expression, or a combination thereof.
• the flow cytometry comprises determining live/dead staining of a cell.
• the in vitro assay comprises ELISA, antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC
• the administered results in an increased antibody-dependent cell-mediated cytotoxicity (ADCC) and/or reduced complement- dependent cytotoxicity (CDC) as compared to the corresponding IgG antibody that comprises the same CDRl, CDR2, and CDR3.
• ADCC antibody-dependent cell-mediated cytotoxicity
• CDC complement- dependent cytotoxicity
• the reduction comprises from 5%, 10%, 15%, 20%., 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, to 100% of the side effect as compared to administering a comparable IgG antibody that comprises the same CDRl, CDR2, and CDR3.
• the antibody or fragment thereof binds a target present on a non-cancerous cell, a cancer cell, or a combination thereof. In some cases, the antibody or fragment thereof binds a target from a brain-metastasizing cancer. In some cases, the antibody or fragment thereof binds one or more of GD2, ALK, hNET, GD3, and CD20.
• the GD2 positive tumor is neuroblastoma, retinoblastoma, melanoma, small cell lung cancer, glioblastoma, osteosarcoma, rhabdomyosarcoma, Ewing's sarcoma, liposarcoma, fibrosarcoma, leiomyosarcoma, and any combinations thereof.
• the antibody or fragment thereof binds a neuroblastoma cell.
• the ALK (anaplastic lymphoma kinase) positive tumor is an anaplastic large-cell lymphoma, an adenocarcinoma of the lung, a neuroblastoma, an inflammatory myofibroblastic tumor, a renal cell carcinomas, esophageal squamous cell carcinoma, breast cancer, a colonic adenocarcinoma, a glioblastoma multiforme or an anaplastic thyroid cancer.
• the hNET (human norepinephrine transporter) positive tumor is a bladder tumor, breast tumor, prostate tumor, carcinoma, leukemia, liver cancer, lung cancer, lymphoma, Hodgkin's lymphoma, Non-Hodgkin's lymphoma, melanoma, neuroblastoma, ovarian tumor, pancreatic tumor or a retinoblastoma.
• the GD3 positive tumor is a neuroectodermal tumor of the center nervous system, glioma, neuroblastoma, retinoblastoma, ependymoma, sarcoma, melanoma, breast cancer, ovarian cancer, glioblastoma, Ewing's sarcoma, or small cell lung carcinoma.
• the CD20 positive tumor is a leukemia, a lymphoma or a neuroblastoma.
• composition comprising an engineered antibody or fragment thereof and a pharmaceutically acceptable carrier.
• kit comprising an engineered antibody or fragment thereof and instructions for use thereof.
• a method comprising: administering to a subject a pharmaceutical composition comprising an antibody or fragment thereof comprising: (a) CDRl, CDR2, and CDR3 of an immunoglobulin G (IgG); and (b) the constant regions or portion thereof of an immunoglobulin A (IgA), wherein the administering results in a reduction of a side effect in the subject as compared to administering a comparable IgG antibody that comprises the same CDRl, CDR2, and CDR3.
• an engineered immunoglobulin G (IgG) antibody fragment wherein the engineered IgG antibody fragment comprises at least a hinge domain and a CH2 domain of a human IgA antibody.
• the engineered IgG antibody fragment when administered to a subject, results in increased antibody- dependent cell-mediated cytotoxicity (ADCC) and/or reduced complement-dependent cytotoxicity (CDC) as compared to a comparable antibody fragment absent the hinge domain and a Cn2 domain of a human IgA antibody
• a method of treating pain or allodynia associated with IgG administration comprising administering to a subject an engineered IgG antibody or fragment thereof comprising the constant regions of an IgA antibody.
• a method of reducing complement activation resulting from IgG administration comprising administering to a subject an engineered IgG antibody or fragment thereof comprising the constant regions of an IgA antibody.
• Gangliosides are sialic acid-containing glycosphingolipids that play important roles in signal transduction as well as cell adhesion and recognition.
• the ganglioside GD2 is a b-series ganglioside that requires the enzymes GD3 synthase and GD2 synthase to add sialic acid units onto its precursor GM2.
• Normal tissues generally express a-series gangliosides, whereas b-series gangliosides are expressed during fetal development and are restricted primarily to the nervous system in healthy adults and at low levels in peripheral nerves and skin
• a structure of the ganglioside GD2 is depicted in figure 10.
• the ganglioside GD2 is highly expressed on neuroectoderm-derived tumors and sarcomas, including neuroblastoma, retinoblastoma, melanoma, small cell lung cancer, brain tumors, osteosarcoma, rhabdomyosarcoma, Ewing's sarcoma in children and adolescents, as well as liposarcoma, fibrosarcoma, leiomyosarcoma and other soft tissue sarcomas in adults.
• GD2 expression in normal individuals appears to be limited to the brain and certain peripheral nerves and melanocytes. As the brain is typically not accessible for normal circulating antibodies GD2 is considered an attractive target for tumor-specific therapy (For review see
• Mahiuddin et al 2014; FEBS letters 588: 288-297). Mahmddin et al describe various GD2 targeted approaches among which there are the GD2 specific antibodies. A number of targeted therapies has reached the clinic and show promise in phase I, II and III trials.
• GD2 antibodies are presently under development.
• the antibodies are thought to induce ADCC and CDC, for which in particular neuroblastoma appears to be relatively sensitive.
• Various avenues are being pursued to improve the efficacy and reduce the toxicity of the targeted approaches.
• the most frequently used antibodies all originate from murine IgG3 antibodies.
• the murine antibodies have been humanized in recent years. A number of variants have been made.
• Murine antibody 14.18 has been chimerized to chl4.18 and humanized into hul4.18 both have a human IgGl background (Mahiuddin et al., 2014; FEBS letters 588: 288-297).
• the murine IgG3 antibody 3F8 has been used in humans. To reduce human anti-mouse responses and improve the efficacy of the antibody while reducing toxicity 3F8 was chimerized (ch3F8) and humanized 3F8 (hu3F8-IgGl and hu3F8-IgG4). In GD2 binding studies by SPR, ch3F8 and hu3F8 maintained KD comparable to m3F8.
• m3F8, ch3F8 and hu3F8 had substantially slower koff. Similar to m3F8, both ch3F8 and hu3F8 inhibited tumor cell growth in vitro, while cross-reactivity with other gangliosides was comparable to that of ni3F8. Both peripheral blood mononuclear cell (PBMC)- ADCC and polymorphonuclear leukocytes (PMN)-ADCC of ch3F8 and hu3F8-IgGl were more potent than m3F8. Hu3F8-IgG4 had near absent PBMC-ADCC and CDC.
• PBMC peripheral blood mononuclear cell
• PMN polymorphonuclear leukocytes
• Hu3F8 and m3F8 had similar tumor-to-non tumor ratios in biodistribution studies. Anti-tumor effect against neuroblastoma xenografts was better with hu3F8-IgGl than m3F8 (see Cheung et al., 2012; Oncoimmunology 1.4: 477-486).
• An antibody (Ab) also known as an immunoglobulin (Ig), is a large protein.
• An antibody interacts with various components of the immune system. Some of the interactions are mediated by its Fc region (located at the base), which contains site(s) involved in these interactions.
• Antibodies are proteins belonging to the immunoglobulin superfamily. They typically have two heavy chains and two light chains. There are several different types of antibody heavy chains that define the five different types of crystallizable fragments (Fc) that may be attached to the antigen-binding fragments. The five different types of Fc regions allow antibodies to be grouped into five isotypes. An Fc region of a particular antibody isotype is able to bind to its specific Fc receptor (FcR) thus allowing the antigen- antibody complex to mediate different roles depending on which FcR it binds.
• FcR Fc receptor
• an IgG antibody to bind to its corresponding FcR is modulated by the presence/absence of interaction sites and the structure of the glycan(s) (if any) present at sites within its Fc region.
• the ability of antibodies to bind to FcRs helps to direct the appropriate immune response for each different type of foreign object they encounter.
• an antibody of the invention is typically a full-length antibody.
• the term 'full length antibody' is defined as comprising an essentially complete
• a full length antibody has two heavy and two light chains. Each chain contains constant (C) and variable (V) regions.
• a heavy chain of a full length antibody typically comprises a CHI, a Cii2, a Cn3, a VH region and a hinge region.
• a light chain of a full length antibody typically comprises a CL region and a VL region.
• An antibody binds to antigen via the variable region domains contained in the Fab portion.
• An antibody variable domain comprises a heavy chain variable region and a light chain variable region.
• Full length antibodies according to the invention encompass heavy and light chains wherein mutations may be present that provide desired characteristics. Full length antibodies should not have deletions of substantial portions of any of the regions. However, IgG molecules wherein one or several amino acid residues are substituted, inserted, deleted or a combination thereof, without essentially altering the antigen binding
• a 'full length antibody can have a substitution, insertion, deletion or a combination thereof, of between 1 and 10 (inclusive) amino acid residues, preferably in non-CDR regions, wherein the deleted amino acids are not essential for the binding specificity of the antibody.
• the four human IgG isotypes bind the activating Fey receptors (FcyRI, FcyRIIa, FcyRIIIa), the inhibitory FcyRIIb receptor, and the first component of complement (Clq) with different affinities, yielding very different effector functions. Binding of IgG to the FcyRs or Clq depends on residues located in the hinge region and the Gij2 domain. Two regions of the Cu2 domain are critical for FcyRs and Clq binding, and have unique sequences in IgG2 and IgG4.
• IgGl antibodies have a Clq binding site through which complement activation through the classical pathway is achieved. IgA antibodies can also activate complement.
• ADCC activity of a murine, murine chimerized or humanized IgG or human IgM GD2 antibody with unmodified constant regions can be increased by replacing at least the hinge domain and the CH2 domain thereof by a hinge domain and Cn2 domain of a human IgA antibody, preferably a human IgAl antibody.
• the Cri3 domain is of a human IgA antibody.
• the antibody comprises an essentially complete IgA constant region.
• the invention therefore provides an antibody that can bind GD2 and that comprises ADCC activity and that comprises a hinge domain and Ci-i2 domain of a human IgA antibody.
• a GD2 antibody of the invention has a reduced capacity to induce CDC.
• a GD2 antibody of the invention has a reduced capacity to induce pain when compared to the murine, murine chimerized or humanized IgG or human IgM original GD2 antibody.
• An IgA constant domain or hinge region may have one or more amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to a germ line IgA domain or hinge region.
• An IgA domain or hinge region preferably has at most 4 amino acid insertions, deletions, substitutions, additions or a combination thereof, preferably at most 3; 2 or preferably at most 1 amino acid insertions, deletions, substitutions, additions or a combination thereof.
• Such a protein is still an IgA constant domain or hinge region.
• An IgA constant domain or hinge region may have one or more amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to a germ line IgA.
• the IgA constant part (including the three domains and the hinge region) can have 0; 1; 2; 3; 4; 5; 6 ;7; 8; 9; 10; 11; 12; 13; 14; or 15 amino acid insertions, deletions, substitutions, additions or a combination thereof.
• the invention provides an anti-ganglioside GD2 antibody that comprises an antibody variable domain and antibody constant domains, wherein the variable domain comprises a heavy and light chain variable region comprising respectively at least the CDR3 of the heavy and light chain variable regions of antibody chl4.18 and an IgA hinge and Cri2 domain.
• the variable domain preferably comprises a heavy and light chain variable region comprising respectively at least the CDR1, CDR2 and CDR3 of the heavy and light chain variable regions of antibody chl4.18 as depicted in figure 1.
• the variable domain preferably comprises a heavy and light chain variable region of antibody chl4.18 as depicted in figure 1.
• the anti-ganglioside GD2 antibody heavy chain variable region is preferably a heavy chain variable region of antibody chl4.18 with 0-5 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody chl4.18 indicated in figure 1.
• the one or more positions are preferably not positions in the CDR1, CDR2 and CDR3 regions.
• the sequence of the CDRs is thus as indicated for heavy chain variable region of antibody chl4.18 of figure 1.
• the heavy chain variable region has 0-4 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDR1, CDR2 and CDRS regions. It is preferred that the heavy chain variable region has 0-3, more preferably 0-2, more preferably 0- 1 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDR1, CDR2 and CDRS regions. In a preferred embodiment a heavy chain variable region in the antibody of the invention has 0 amino acid insertions, deletions, substitutions, additions or a combination thereof with respect to the heavy chain variable region sequence of antibody chl4.18 in figure 1.
• the anti-ganglioside GD2 antibody light chain variable region is preferably a light chain variable region of antibody chl4.18 with 0-5 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody chl4.18 indicated in figure 1.
• the one or more positions are preferably not positions in the CDRl, CDR2 and CDRS regions.
• the sequence of the CDRs is thus as indicated for light chain variable region of antibody chl4.18 of figure 1.
• the light chain variable region has 0-4 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDRS regions. It is preferred that the light chain variable region has 0-3, more preferably 0-2, more preferably 0-1 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDRS regions. In a preferred embodiment a light chain variable region in the antibody of the invention has 0 amino acid insertions, deletions, substitutions, additions or a combination thereof with respect to the light chain variable region sequence of antibody chl4.18 in figure 1.
• the invention provides an anti-ganglioside CD2 antibody that comprises an antibody variable domain and antibody constant domains, wherein the variable domain comprises a heavy and light chain variable region comprising respectively at least the CDR3 of the heavy and light chain variable regions of antibody 3F8 and an IgA hinge and CH2 domain.
• the variable domain preferably comprises a heavy and light chain variable region comprising respectively at least the CDR1, CDR2 and CDR3 of the heavy and light chain variable regions of antibody 3F8 (as depicted in figure 1).
• the variable domain preferably comprises a heavy and light chain variable region of antibody 3F8 as depicted in figure 1.
• the anti-ganglioside GD2 antibody heavy chain variable region is preferably a heavy chain variable region of antibody 3F8 with 0-5 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody 3F8 indicated in figure 1.
• the one or more positions are preferably not positions in the CDR1, CDR2 and CDR3 regions.
• the sequence of the CDRs is thus as indicated for heavy chain variable region of antibody 3F8 of figure 1.
• the heavy chain variable region has 0-4 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody 3F8 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDRS regions. It is preferred that the heavy chain variable region has 0-3, more preferably 0-2, more preferably 0-1 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody 3F8 indicated in figure 1, wherein the one or more positions are not positions in the CDR1, CDR2 and CDR3 regions. In a preferred embodiment a heavy chain variable region in the antibody of the invention has 0 amino acid insertions, deletions, substitutions, additions or a combination thereof with respect to the heavy chain variable region sequence of antibody 3F8 in figure 1.
• the anti-ganglioside GD2 antibody light chain variable region is preferably a light chain variable region of antibody 3F8 with 0-5 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody 3F8 indicated in figure 1.
• the one or more positions are preferably not positions in the CDR1, CDR2 and CDR3 regions.
• the sequence of the CDRs is thus as indicated for light chain variable region of antibody 3F8 of figure 1.
• the light chain variable region has 0-4 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody 3F8 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDR3 regions. It is preferred that the light chain variable region has 0-3, more preferably 0-2, more preferably 0-1 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody 3F8 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDR3 regions. In a preferred embodiment a light chain variable region in the antibody of the invention has 0 amino acid insertions, deletions, substitutions, additions or a combination thereof with respect to the light chain variable region sequence of antibody 3F8 in figure 1.
• IgA has two subclasses (IgAl and IgA2) and can be produced as a
• the antibody in the present invention is preferably a monomeric antibody.
• the IgA elements in an antibody of the invention are preferably human IgA elements.
• An IgA element can be an IgAl element or an IgA2 element.
• IgA elements in an antibody of the invention can be all IgAl elements or all IgA2 elements or a combination of IgAl and IgA2 elements.
• An IgA element is preferably a human IgA element.
• Preferably all IgA element in the antibody are human IgA elements.
• the IgA elements can be IgAl elements, preferably human IgAl elements.
• the IgA elements can also be IgA2, preferably IgA2m(l) elements, preferably human IgAl elements.
• the Cul domain and/or CH3 domain of the antibody can be an IgG Cul domain, an IgG Ci-i3 domain or a combination thereof. It is preferred that the Cul domain, CH3 domain or
• the IgA CHI domain and/or hinge region is a human IgA Cul domain and/or human IgA hinge region.
• Said human IgA Cul domain and/or human IgA hinge region is preferably an human IgAl CH I domain or human IgAl hinge region.
• Said human IgA Cul domain and/or human IgA hinge region is preferably an human IgA2m(l) CHI domain or human IgA2m(l) hinge region.
• the constant domains and hinge region of the antibody are preferably human constant regions and hinge region, preferably of a human IgA antibody.
• the constant domains and hinge region of the antibody are preferably human IgAl or human IgA2m(l) constant domains and hinge region.
• a human constant region can have 0- 15 amino acid changes with respect to a human allele as found in nature.
• An amino acid change may be introduced for various reasons. Non-limiting examples include but are not limited to improving production or homogeneity of the antibody, adapting half-life in the circulation, stability of the HC/LC combination, optimizing glycosylation, adjusting
• a human constant region can have 0; 1; 2; 3; 4; 5; 6; 7; 8; 9; 10; 11; 12; 13; 14; and 15 ammo acid changes with respect to a human allele as found in nature.
• the changed amino acid is preferably one chosen from an amino acid at a corresponding position of a different isotype.
• the constant regions of the heavy chain are IgA2 constant regions, preferably human IgA2 constant regions, preferably human IgA2m(l).
• the human constant region is a mutated IgA2m(l) sequence.
• the antibody comprises the constant regions of an IgA2m(l) sequence, preferably with at least one and preferably at least 2; 3; 4; 5; and preferably at least 7 of the following mutations: N166G; P221R; N337T; I338L; T339S; C331S; and mutation of the C-terminal amino acid sequence which is a human IgA2m(l) antibody is "... VDGTCY" into "... VDGT.
• Figure 13 shows the sequence of human IgAl; IgA2m(l) and a preferred mutated IgA2m(l) sequence (hIgA2.0); see also figure 13E.
• the GD2 antibody comprises a heavy chain with the constant regions of figure 13E with 0; 1; 2; 3; 4; 5; 6; 7; 8; 9; 10; 11; 12; 13; 14; and 15 amino acid changes with respect to the sequence provided in figure 13E, provided that amino acids at positions 166; 221; 337; 338; 339; and 331 are 166G; 221R; 337T; 338L; 339S and 331S.
• the C-terminal ammo acid sequence which is a human IgA2m(l) antibody is "...VDGTCY" is "...VDGT".
• the heavy chain is subject to isotype switching.
• the mention IgA antibody preferably comprises a chl4.18 variable domain or a 3F8 variable domain, preferably a chl4.18 variable domain.
• An anti-ganglioside GD2 antibody comprising:
• the heavy chain variable region has 0-4 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDR3 regions. It is preferred that the heavy chain variable region has 0-3, more preferably 0-2, more preferably 0- 1 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the heavy chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDRl, CDR2 and CDR3 regions. In a preferred embodiment a heavy chain variable region in the antibody of the invention has 0 amino acid insertions, deletions, substitutions, additions or a combination thereof with respect to the heavy chain variable region sequence of antibody chl4.18 in figure 1;
• the light chain variable region has 0-4 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDR1, CDR2 and CDRS regions. It is preferred that the light chain variable region has 0-3, more preferably 0-2, more preferably 0-1 amino acid insertions, deletions, substitutions, additions or a combination thereof at one or more positions with respect to the light chain variable region sequence of antibody chl4.18 indicated in figure 1, wherein the one or more positions are not positions in the CDR1, CDR2 and CDR3 regions. In a preferred embodiment a light chain variable region in the antibody of the invention has 0 amino acid insertions, deletions, substitutions, additions or a combination thereof with respect to the light chain variable region sequence of antibody chl4.18 in figure 1;
• an IgA heavy chain preferably comprising an amino acid sequence as depicted in figure 13E with 0; 1; 2; 3; 4; 5; 6; 7; 8; 9; 10; 11; 12; 13; 14; and 15 amino acid changes with respect to the sequence provided in figure 13E, provided that amino acids at positions 166; 221; 337; 338; 339; and 331 are 166G; 221R; 337T; 338L; 339S and 33 IS.
• the C-terminal amino acid sequence which is a human IgA2m(l) antibody is "...VDGTCY" is "...VDGT"; and
• the anti-ganglioside GD2 antibody of the invention preferably exhibits more antibody-dependent cell-mediated cytotoxicity (ADCC) than the antibody dinutuximab when measured in a suitable in vitro ADCC assay. It preferably exhibits less complement-dependent cytotoxicity (CDC) than the antibody dinutuximab when measured in a suitable in vitro CDC assay.
• ADCC antibody-dependent cell-mediated cytotoxicity
• CDC complement-dependent cytotoxicity
• Various ADCC and CDC assays are available to the person skilled in the art. In the context of the present invention it is preferred that an ADCC assay or a CDC assay as described in the examples is used. ADCC function of an antibody as claimed is preferably measured in a classical chromium release assay (as described for instance in examples).
• CDC function of an antibody as claimed is preferably measured in a method based on 7-AAD positivity in flow cytometry (see for instance the examples).
• the antibody preferably exhibits 20% or less, more preferably 10% or less of the complement-dependent cytotoxicity (CDC) of the antibody dinutuximab when measured in a suitable in vitro CDC assay.
• the antibody comprises an albumin-binding domain (ABD) attached to a heavy or light chain of the antibody.
• ABD albumin-binding domain
• Another domain is added to increase the half- life of the antibody.
• the domain the Dili domain of human albumin. Such a domain is preferably physically linked to a constant region of an IgA part of the antibody.
• an engineered antibody or fragment thereof, as provided herein comprising the variable region of an IgG antibody and the constant regions of an IgA antibody, can reduce a side effect.
• an engineered antibody or fragment thereof, as provided herein can reduce a side effect associated with an IgG antibody therapy.
• an engineered antibody or fragment thereof, as provided herein can reduce a side effect associated with an innate immune response. Side effects associated with such innate immune response can be inflammation, complement system activation, white blood cell (mast cell, phagocyte, macrophage, neutrophil, dendritic cell, basophil, eosinophil, natural killer cell, and gamma delta cell activity.
• an engineered antibody or fragment thereof, as provided herein can reduce a side effect associated with complement system activation.
• Complement system activation can be a biochemical cascade of the immune system that complements the ability of antibodies or fragments thereof to clear pathogens or mark them for destruction.
• the complement cascade comprises a variety of plasma proteins, which are synthesized in the liver, these proteins have various functions comprising: triggering the recruitment of inflammatory cells, tagging pathogens for destruction (opsonization), perforating the plasma
• a reduction of a side effect can comprise the reduction of complement system activation.
• an IgG antibody or portion thereof can react with a target resulting in binding of Clq to the Fc portion of antigen-bound IgG after which Clr and Cls attach to form CI, an enzyme associated with complement activation.
• CI can proceed to enzymatically cleave C4 into C4a and C4b.
• C4b binds to adjacent proteins and carbohydrates on the surface of the target and then binds C2.
• Activated CI can cleave C2 into C2a and C2b forming C4b2a, a C3 convertase.
• C3 convertase can cleave C3 into C3a and C3b.
• C3b can bind to C4b2a, a C3 convertase, to form C4b2a3b, a C5 convertase that can cleave C5 into C5a and C5b.
• C5b can bind to the target and also bind C6, C7, c8, and C9 to form C5b6789n, the membrane attack complex (MAC).
• MAC membrane attack complex
• MAC can destroy gram-negative bacteria as well as cells displaying foreign antigens (such as virus-infected cells, tumor cells, to name a few) by causing their lysis.
• a reduction in a complement response can be measured by the absence or reduced level of any one of: Clq, Clr, Cls, CI, C4, C4a, C4b, C2, C2a, C2b, C4b2a, C3, C3a, C3b, C4b2a3b, C5, C5a, C5b, C6, C7, C8, C9, C5b6789m, MAC, and any combination thereof.
• a reduction in a complement response can refer to a reduction of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, 100% of bmdmg of a complement factor selected from any one of: Clq, Clr, Cls, CI, C4, C4a, C4b, C2, C2a, C2b, C4b2a, C3, C3a, C3b, C4b2a3b, C5, C5a, C5b, C6, C7, C8, C9, C5b6789m, and MAC to the engineered antibody that comprises an IgG variable region and the IgA constant regions or portion thereof as described herein, as compared to a comparable amount of an IgG antibody.
• a complement factor selected from any one of: Clq, Clr, Cls, CI, C4, C4a, C4b, C2, C2a,
• a reduction of a complement response can be determined by performing an ELISA, flow cytometry, hematology, or an in vitro assay to quantify an amount of a complement factor selected from any one of: Clq, Clr, Cls, CI, C4, C4a, C4b, C2, C2a, C2b, C4b2a, C3, C3a, C3b, C4b2a3b, C5, C5a, C5b, C6, C7, C8, C9, C5b6789m, MAC, and any combination thereof.
• an antibody or fragment thereof provided herein can result in a lower level of a complement factor as compared to a comparable antibody or fragment thereof comprising the provided CDR1, CDR2, and CDR3 regions.
• an engineered IgG-IgA antibody or fragment thereof provided herein can result in a lower level of a complement factor as compared to a comparable IgG antibody or fragment thereof comprising the same CDR.1, CDR.2, and CDR3.
• a reduction of a complement response can also be measured by flow cytometric quantification of complement dependent lysis of target cells as well as described in the examples.
• Additional side effects which can be mitigated or reduced by administration of an engineered antibody or fragment thereof described herein, as compared to administration of an IgG, can refer to any side effects associated with IgG antibody therapy.
• Side effects associated with IgG antibody therapy can include
• an IgG antibody therapy can result in side effects such as: inflammation, hypertension, hypotension, pain, lever, urticaria, allergy, chill, weakness, diarrhea, nausea, vomit, rash, itch, cough, constipation, edema, headache, fever, shortness of breath, muscle ache, pain, decreased appetite, insomnia, dizziness, anaphylaxis, thrombosis, heart failure, bleeding, hepatitis, enterocolitis, mucositis, cytokine syndrome, hypothyroidism, hyponatremia, hypokalemia, capillary leak syndrome, and allodynia.
• an antibody or fragment, thereof provided herein, such as engineered antibodies can be administered at a higher dose, a longer treatment period, or with more frequency as compared to a comparable IgG antibody comprising the same CDR1, CDR2, and CDR3 regions.
• an antibody or fragment thereof provided herein, such as engineered antibodies can be administered at a higher dose from about IX, 2X, 3X, 4X, 5X, 6X, 7X, 8X, 9X, 10X, over that of a comparable IgG antibody comprising the same CDR.1, CDR.2, and CDR3 regions.
• an antibody or fragment thereof provided herein, such as engineered antibodies can be administered for a longer time period from about 1 hour, 5 hrs, 10 lirs, 1 day, 2 days, 3 days, 4 days, 5 days, 7 days, 14 days, 24 days, 30 days, monthly, bimonthly, bi-yearly, yearly, and daily over that of a comparable IgG antibody comprising the same CDR1, CDR2, and CDR3 regions.
• an antibody or fragment thereof provided herein, such as engineered antibodies can be administered more frequency such as hourly, daily, weekly, monthly, yearly as compared to a comparable IgG antibody comprising the same CDR1, CDR2, and CDR3 regions.
• A. reason for changing an amino acid at a certain position can be
• Antibodies of the present invention have variable heavy and variable light chain regions derived from a murine background.
• Antibodies with such variable domains can be used in humans. Presently it is preferred to de-immunize such variable domains. De-immunization typically involves the modification of the murine sequence into a more human sequence whenever possible. Typically such modifications are directed towards removing one or more T-ceii epitopes or one more B-ceil epitopes from the variable domain. In a preferred embodiment one or more (human) T-cell epitopes have been removed by replacement of at least one amino acid of the epitope with a different amino acid. Often it is sufficient to substitute the so-called “anchor" amino acid. Suitable replacement amino acids can be obtained from somatic cell hypermutants of the particular VH or VL.
• human B-eell epitopes can be removed by replacement of at least one amino acid of the epitope with a different amino acid. Often it is sufficient, to substitute only one amino acid of the epitope. Suitable replacement amino acids can be obtained from somatic cell hypermutants of the particular VH or VL. Replacement with an amino acid that is naturally present at that position in a human antibody is preferred.
• a variable domain of the invention is modified with respect to one or more exterior residues. Such residues are readily encountered by the immune system and are preferably selectively replaced with human residues to provide a hybrid molecule that comprises either a weakly immunogenic or substantially non-immunogenic surface.
• Suitable replacement amino acids can be obtained from somatic cell hypermutants of the particular VH or VL. Replacement with an amino acid that is naturally present at that position in a human antibody is preferred.
• the invention thus further provides an antibody of the invention that comprises a humanized heavy chain variable region, a humanized light chain variable region or a combination thereof.
• a light chain of an antibody as defined herein has a light chain constant region.
• the light chain constant region is preferably the light chain constant region of the corresponding antibody.
• a light chain comprising a chl4.18 light chain variable region preferably also comprises the light chain constant region of the chl4.18 antibody.
• a light chain comprising a 3F8 light chain variable region preferably also comprises the light chain constant region of the 3F8 antibody.
• the invention further provides a method of treatment of a subject that has a GD2 positive tumor or is at risk of having said GD2 positive tumor the method comprising administering a therapeutic amount of an antibody of the invention to the subject in need thereof.
• an antibody of the invention for use in the treatment of a subject that has a GD2 positive tumor or is at risk of having a GD2 positive tumor.
• the treatment preferably further comprises administering retinoic acid in an amount effective to upreguiate the ganglioside GD2 in neuroblastoma cells in said subject.
• the treatment preferably further comprises administering granulocyte-macrophage colony-stimulating factor (GM-CSF), G- GSF or a combination thereof in an amount effective to increase the number of granulocytes in the subject and/or to increase ADCC.
• GM-CSF granulocyte-macrophage colony-stimulating factor
• G-CSF granulocyte-macrophage colony-stimulating factor
• the GM-CSF, G-CSF or a combination thereof is preferably of a species that is to be treated. It is preferably human GM-CS F, human G-CSF or a combination thereof.
• the GD2 positive tumor is preferably a GD2 positive neuroblastoma such as a neuroectoderm-derived tumor or a sarcoma.
• the GD2 positive tumor is a GD2 positive neuroblastoma, retinoblastoma, melanoma, small cell lung cancer, brain tumor, osteosarcoma, rhabdomyosarcoma, E wing's sarcoma in children and adolescents, or liposarcoma, fibrosarcoma, leiomyosarcoma or another soft tissue sarcoma in adults.
• the GD2 positive tumor is a neuroblastoma.
• the neuroblastoma treated with a method or use of the invention is preferably a high risk neuroblastoma.
• the retinoic acid is preferably 13-cis-retinoic acid (isotretinoin).
• the invention further provides an antibody of the invention or a me thod or antibody for use of the invention, wherein the antibody comprises a heavy chain variable region with the amino acid sequence
• the invention also provides a nucleic acid molecule or combination of nucleic acid molecules that codes for a heavy chain, a light or preferably both of an antibody as described herein.
• the nucleic acid molecule or combination preferably further comprises one or more sequences for the expression of an antibody as described. Non-limiting examples of such expression sequences are a promoter, a termination sequence, an enhancer, an intron etc.
• sequences are not necessarily present on the nucleic acid molecule as such sequences can be provided in cis by the integration site of the nucleic acid molecule in, for instance, a chromosome of a cell, or a vector comprising said nucleic acid molecule.
• Suitable integration sites in a cellular chromosome can easily be determined and targeted, for instance by means of homologous recombination.
• a cell that comprises a nucleic acid molecule or combination as described herein.
• a nucleic acid molecule or combination according to the invention is for instance comprised in a cell.
• the translated product of the nucleic acid molecule can be, or be incorporated into, an antibody of the invention.
• the invention thus also provides a cell comprising a nucleic acid molecule or combination according to the invention.
• the invention further provides a cell comprising a nucleic acid molecule or combination of the invention and that is capable of producing an antibody of the invention.
• a method for producing an antibody of the invention comprising culturing a cell comprising expressing one or more nucleic acid molecules that code for an antibody of the invention and harvesting the antibody from the culture medium, the cell or a combination thereof.
• Said cell is preferably an animal cell, more preferably a mammalian cell.
• the cell is preferably a cell that is normally used for the production of an antibody for use in humans.
• Non-limiting examples of such cells are CHO, NSO, HEK cells preferably HEK293F cells, and PER.C6 cells.
• Cells may specifically designed to suit certain purposes, for instance, most cell lines used for the production of antibodies have been adapted for growth in suspension, in high densities and other properties.
• a suitable cell is any cell capable of comprising and preferably of producing an antibody according to the invention.
• Figure 1 Variable heavy and light chain amino acid of the antibodies chl4.18 (panel A and B respectively; the CDR regions are underlined). DNA sequences coding for the variable heavy and light chain of the antibody chl4.18 (panel C and D respectively). For 3F8 a chimeric gammal heavy chain (SEQ ID NO: 1) and a chimeric kappa light chain (SEQ ID NO: 2) are depicted. The respective CDRs are underlined.
• Figure 2 Quantification of IgA chl4.18 after transiection with several heavy (HC) and light chain (LC) ratio's. 5 different ratios were used for IgGl and IgAl production. The optimal ratios were used for larger scale production.
• HC heavy
• LC light chain
• Figure 3 A: ⁇ -light chain specific affinity chromatography of IgA chl4.18.
• B Size exclusion chromatography of IgA chl4.18 UV absorption, representing protein concentration, is indicated by the trace.
• Figure 4 binding of in-house produced and purified IgGl and IgA chl4.18 to the GD2 expressing neuroblastoma cell line IMR32 and SK-N-FI in flow cytometry.
• Figure 5 CDC assay of IgGl and IgA after 1 hr and 4 hrs of incubation with IMR32 cell line. Cell lysis through complement activation is analyzed by flow cytometric analysis. 15% pooled human serum was added, and cells were incubated for 60 min and 4 hrs at 37°C. The amount of cell lysis is measured by 7-AAD staining.
• Figure 6 Leukocyte (Panel A and C) and PMN (Panel B and D)ADCC assay of IgGl and IgA after 4 hrs of incubation with the IMR32 (Panel A and B) and SK-N- FI (Panel C and D) cell lines. Red blood cells were lysed and the remaining effector cells were added to wells. After 4 hours of incubation at 37°C, 5I Cr release was measured in counts per minute (cpm) by a beta-gamma counter. The percentage of specific lysis was calculated by determining the maximal lysis in the presence of triton and basal lysis in the absence of antibodies and effector cells.
• FIG 7 Leukocyte ADCC assay of in-house produced and purified IgGl and IgA after 4 hrs of incubation with the IMR32 and SK-N-FI cell line. Red blood cells were lysed and the remaining effector cells were added to wells together with cytokines. After 4 hours of incubation at 37°C, 51 Cr release was measured in counts per minute (cpm) by a beta-gamma counter. The percentage of specific lysis was calculated by determining the maximal lysis in the presence of triton and basal lysis in the absence of antibodies and effector cells.
• Figure 8 PMN ADCC assay of IgGl and IgA chl4.18 after 4 hrs of incubation with the IMR32 or SK-N-FI cell line.
• PMNs were isolated by Ficoll/Histopaque separation. Subsequently, effector cells, cytokines and antibodies at various concentrations were added to microtiter plates containing target cells. E:T ratios were 40: 1 (PMN). After 4 hours of incubation at 37°C, 51 Cr release was measured in counts per minute (cpm) by a beta-gamma counter. The percentage of specific lysis was calculated by determining the maximal lysis in the presence of triton and basal lysis in the absence of antibodies and effector cells.
• Figure 9 GD2 (Panel A) and MHC-I (Panel B) expression on IMR32 cells after exposure to different concentrations of isotretinoin for 4 consecutive days. Cells were stained with IgA chl4.18 and detected with a secondary anti-IgA PE or anti- MHC-I-PE.
• Figure 10 Schematic representation of the ganglioside GD2.
• Figure 11 Quantification of in vivo mechanical thresholds. Experiments were conducted using female (aged 8-12 weeks) C57BL/6 mice (Harlan Laboratories). Mice received an intravenous injection of 100 microgram of antibody. Mechanical thresholds were determined using the von Frey test (Stoelting) with the up-and- down method as is described in Eijkelkamp et al., 2010; J. Neurosci. 30:2138-2149 and Chaplan et al., 1994; J. Neurosci. Methods 53:55-63. All experiments were performed by experimenters blinded to treatment.
• Figure 12 Primary sequence and modeling of the IgAl/IgA2.0 hybrid antibody.
• B the heavy chain of 225-IgA2.() was modeled and illustrated in front and side view, with mutations marked.
• C heavy chains of wild-type and mutant IgA2 were modeled. The resulting alignment indicates a different orientation of C241 in the heavy chains of IgA2-wt compared with IgA2.0, possibly due to the P221R mutation.
• D focus on the tailpiece of 225-IgA2-wt (green, C471; red, Y 472) and IgA2.0 (red). Prediction and alignment of models were performed using I-TASSER; models were modified in 3D-Mol Viewer.
• E nucleic acid sequence and protein sequence of the constant regions of the heavy chain of IgA2.0.
• FIG. 13 HP-SEC analysis of IgAl and IgGl chl4.18. Purified antibodies were subjected to HP-SEC analysis. Both IgAl chl4.18 (A) and IgGl chl4.18 (B) showed to be highly monomeric. Traces show the UV absorbance at 280 nm.
• Figure 14 Binding of IgGl and IgAl chl4.18 antibodies to neuroblastoma cell lines. (A) Antibody binding of IgAl-FITC and IgGl-FITC chl4.18 to GD2
• FIG. 15 Characterization of ADCC by IgAl and IgGl chl4.18 against a panel of neuroblastoma cell lines.
• A ADCC assays with IgAl and IgGl cb.14.18 on 3 different neuroblastoma cell lines with leukocytes from peripheral blood as effector cells.
• C ADCC assays with IgGl chl4.18 on IMR32 cell line with isolated PBMC's (E:T ratio of 100: 1) or isolated neutrophils (E:T ratio of 40: 1) as effector cells with co- treatment of 10 ng/ml GM-CSF, 6545 U/ml of IL-2 and 24 hours of pre-incubation with 10 ⁇ of 11-cis retinoic acid.
• D ADCC assays with leukocytes from
• Figure 16 Complement assays on a panel of neuroblastoma cell lines by IgGl and IgAl chl4.18 antibodies.
• A Lysis by IgGl chl4.18 antibodies on 4 different neuroblastoma cell lines. Cells were incubated with 4 different concentrations of antibody and 15% serum for 15 minutes and 4 hours.
• B Lysis by IgAl chl4.18 antibodies on 4 different neuroblastoma cell lines. Cells were incubated with 4 different concentrations of antibody and 15% serum for 15 minutes and 4 hours.
• C Expression of complement regulatory proteins CD46, CD55 and CD59 on
• Figure 17 In vivo efficacy of IgGl and IgAl chl4.18 antibodies.
• A Quantification of bioluminescent signal of neuroblastoma cells after 24 hours of treatment with IgAl or IgGl chl4.18.
• B Quantification of bioluminescent signal at 3 days of treatment after I.V. injection of tumor cells.
• Figure 18 Neuronal exposure of to IgAl does not lead to decreases in mechanical withdrawal thresholds.
• A Plasma concentrations of IgAl and IgGl chl4.18 3 hours after intravenous injection.
• B Von-Frey withdrawal thresholds.
• C Von- Frey withdrawal thresholds after I.V. injection of fluorescently labeled IgAl chl4.18 or IgGl chl4.18.
• D Left column: Visualization of intravenously injected Alexa-488 labeled antibodies on sciatic nerves. Right column: Visualization of ex- vivo staining of GD2 by incubation of neurons with Alexa-549 labeled IgGl chl4.18.
• the amino acid sequences of the variable regions from chl4.18 were found in the FDA application of dinutuximab (application 125516; depicted in Figure 1). These amino acid sequences were translated to the cDNA sequence representing the most likely non- degenerate coding sequence.
• the cDNAs were synthesized (Baseclear) and subcloned into the pEE14.4 expression vectors which contain either the IgAl or IgGl backbone (described in: Beyer, T., et al. "Serum-free production and purification of chimeric IgA antibodies.” Journal of immunological methods 346.1 (2009): 26-37).
• the optimal ratio of heavy to light chain DNA for transfection was first determined by small scale test transfections in HEK293F cells. Antibody production was then quantified by anti-human IgG ( Figure 2A) or IgA ELISA ( Figure 2B).
• IgA chl4.18 was isolated from the serum free supernatant using human ⁇ -light chain specific affinity
• Binding of anti-GD2 antibodies to the GD2 expressing cell lines IMR32 and SK-N-FI was analyzed by staining cells on ice for 45 minutes with anti-GD2 antibody at several concentrations. Cells were washed and a secondary goat-anti human IgA-PE or IgG-PE was added to the cells for 45 minutes on ice in the dark. Afterwards, antibody binding was quantified by flow cytometric analysis.
• ADCC assays were performed to assess the efficacy of IgA and IgGl chl4.18 to recruit effector cells against neuroblastoma cells.
• leukocyte ADCC's were performed. Hereto, peripheral blood from healthy donors was treated with RBC Lysis buffer to remove red blood cells.
• the remaining leukocytes were washed and added to radioactively labeled neuroblastoma cells with or without IL-2 and GM- CSF (both lOng/ml). After 4 hours of incubation at 37°C, cell death was quantified by 5I Cr release, measured by liquid scintillation. The percentage of specific lysis was calculated by determining the maximal lysis in the presence of 2.5% triton X- 100 and basal cell lysis in the absence of antibodies and effector cells.
• PMNs were isolated from peripheral blood from healthy donors by ficoll-histopaque and added in a 40: 1 E:T ratio to radioactively labelled neuroblastoma cells with or without IL-2 and GM-CSF (both lOng/ml). Results and discussion
• the functional characterization of the antibodies was performed by determining binding capacity to GD2, complement activation and effector cell recruitment. Flow cytometric analysis of binding to the GD2-positive cell lines IMR32 and SK-N-FI showed similar binding patterns for both IgGl and IgA ( Figure 4). Complement activation of the produced antibodies was assessed by live/dead staining (7-AAD) after incubation with 15% pooled human serum and several concentrations of antibody The IgA anti-GD2 antibody did not show activation of complement, while the IgGl variant having the same variable regions did activate the complement system after 1 hour of incubation (Figure 5A). Lysis increased further for IgG when incubation time increased to 4 hours, while for IgA no complement activation was observed (Figure 5B)
• ADCC assays were performed to assess the efficacy of IgA and IgGl chl4.18 to recruit effector cells against neuroblastoma cells.
• leukocyte ADCC's were performed.
• the IgA anti-GD2 antibody showed to be superior for both the IMR32 and SK-N-FI cell line ( Figure 6).
• PMN were isolated from peripheral blood from healthy donors. It was shown that the IgA anti-GD2 antibody stimulates PMN better than IgGl to kill IMR32 and SK-N-FI cells ( Figure 6).
• the examples show that an IgA isotype variant of dinutuximab has higher ADCC capacity for killing of neuroblastoma's, and no complement activation.
• the IgA anti-GD2 antibody enhances destruction of neuroblastoma cells, whereas side effects such as neuropathic pain are reduced.
• the antibody of the invention provides potent ADCC activity while simultaneously reducing at least the pain problem associated with dinutuximab administration.
• Antibodies of the invention efficiently activate neutrophils presumably through the FcaR (CD89). This generates potent anti- tumor reactions.
• the susceptibility of neuroblastoma cells to an antibody of the invention shows that an IgA anti-GD2 exhibits improved efficacy against neuroblastoma when compared to an IgG isotype variant with the same variable domains, and simultaneously reduces treatment toxicity. This significantly improves anti-neuroblastoma immunotherapy.
• Antibody function is governed by a number of factors.
• the target and the epitope that is recognized play an important role as does the cell on which the target resides.
• Effector functions of the antibody are often correlated with the isotype of the antibody. Although this is useful as a general rule, many exceptions indeed exist. This is exemplified for instance for ADCC activity by Rajasekaran et al (Rajasekaran et al., 2015; ImmunoTargets and Therapy Vol 4: 91) and for CDC activity by Lohse et al., 2017; Br J Haematol. doi: 10.1111/bjh.
• FcaRI Inhibitory receptors and polymorphisms are not reported for FcaRI.
• the FcaRI is not expressed in mice, making it for a long time not possible to do the necessary preclinical in vivo studies with IgA therapeutic antibodies.
• the human FcaRI transgenic mouse was generated. This mouse has a similar expression pattern of human FcaRI as in humans. These mice were back- crossed in the relevant backgrounds, i.e. balb/c, C57B/L6, and SCID for growing human tumors.
• IgA as therapeutic antibody.
• IgA is known as a mucosal antibody, in its monomeric form it is the second class of antibody present in the human serum.
• an anti-tumor antibody IgA can be effective in vitro. The antitumor mechanism is different and mainly through the recruitment of neutrophils, the most abundant type of leucocytes. Also in vivo IgA can be efficacious as a therapeutic antibody.
• a drawback of an IgA molecule is its on average relatively short half-life, due to different glycosylation and lack of binding to the neonatal Fc receptor, FcRn.
• the present invention solves this problem for GD2 specific IgA antibodies by providing them with adapted glycosylation and targeting of the IgA to FcRn indirectly (Meyer et al., 2016 MAbs Vol 8: pp 87-98).
• anti-GD2 IgA isotype antibodies are targeting neuroblastoma, in vitro, in vivo and in patient-derived models.
• the efficacy of the anti-GD2 antibodies to elicit ADCC will be assay against a further panel of GD2 expressing neuroblastoma cell lines (e.g. IMR-32, SH-SY5Y, SK-N-FI, LAN-5), from ATCC) and ex vivo neuroblastoma cells as targets.
• the panel will include tumor-initiating cells that have been generated from primary neuroblastoma samples and that contain stem cell-like cells that may represent the chemotherapy-resistant cell population responsible for resistance to conventional chemotherapy (such as described in Bate-Eya, et al., 2014; European journal of cancer 50.3: 628-637). Both whole blood and isolated effector populations from healthy volunteers and patients will be used as effector cells.
• target cells will be labelled with r, 1 Cr for 2 hours.
• PMNs and PBMCs will be isolated by Ficoll/Histopaque separation.
• effector cells, antibodies at various concentrations, and medium will be added to microtiter plates containing target cells.
• E:T ratios will be 40: 1 (PMN) and 50: 1 (PBMC).
• 51 Cr release will be measured in counts per minute (cpm). The percentage of specific lysis will be calculated by determining the maximal lysis in the presence of triton and basal lysis in the absence of antibodies and effector cells.
• Tumor-derived organoids reflect tumor heterogeneity and allow performing these functional tests on tissues representing primary tumor tissue.
• IgA dinutuximab on these organoids.
• Chl4.18 a chimeric antibody of the IgGl isotype directed against the ganglioside GD2, expressed on neuroblastoma cells, but also on peripheral and central nervous tissue.
• Chl4.18 is given as second-line treatment in combination with IL-2, GM-CSF and 11-cis retinoic acid for the treatment of high-risk
• Chl4.18-antibody-opsonized tumor cells can be killed by leukocytes through antibody-dependent cell-mediated cytotoxicity (ADCC), depending on antibody binding to Fc receptors on leucocytes.
• ADCC antibody-dependent cell-mediated cytotoxicity
• Chl4.18 is also able to activate the complement system on the tumor cell surface, causing lysis of the cell via complement-dependent cytotoxicity (CDC).
• CDC complement-dependent cytotoxicity
• IgGl antibody therapy natural killer (NK) cells are regarded as important cells for mediating ADCC.
• NK natural killer
• neuroblastoma there is evidence that granulocytes also play a role in mediating ADCC when treated with an anti-GD2 IgGl antibody (Bruchelt et al 1989, Immunol Lett 22(3): 217-220; Gilman et al 2009, J Clin Oncol 27(1): 85-91; Cheung et al 2012, J Clin Oncol 30(4): 426-432).
• IgAl and IgGl chl4.18 specifically bind GD2 with similar affinity
• ADCC and CDC are known to be induced by chl4.18 against neuroblastoma in vivo.
• ADCC assays were performed on IMR-32, SK-N-FI and LAN-1 neuroblastoma cell lines with leukocytes as effector cells.
• Both IgA and IgG antibodies lysed IMR-32 cells to a similar extent, while SK-N-FI and LAN- 1 cells were killed better with IgAl chl4.18 ( Figure 15A).
• the GI-ME-N cell line that has no detectable GD2 expression on FACS could not be lysed by both antibodies, showing that GD2 expression is a prerequisite for ADCC (data not shown).
• neutrophils and peripheral blood mononuclear cells were separately used as effector cells to determine their respective cytotoxic capacity against neuroblastoma cell lines with these antibodies.
• IgGl chl4.18 effectively lysed IMR32 cells, while lysis with the IgA antibody did not perform as well ( Figure 15B). This was also seen for 2 other neuroblastoma cell lines (data not shown) On the contrary, when neutrophils were used as effector cells, IgA chl4.18 mediated superior ADCC for all tested cell lines in comparison to IgGl ( Figure 15B).
• GM-CSF in combination with IgAl chl4.18 boosted ADCC with neutrophils as effector cells (Figure 15C). Presence of IL-2 did not further increase neutrophil mediated killing, while maximal lysis increased with preexposure to 11-cis-retinoic acid. For IgGl antibodies, GM-CSF improved killing with neutrophils slightly, while addition of IL-2 or retinoic acid did not enhance killing further (data not shown).
• a second effect mediated by chl4.18 is activation of the complement system.
• Chl4.18 is known to lyse neuroblastoma target cells via CDC in vitro. We assessed in vitro complement activation by these antibodies on the same panel of
• EL4 cells were intravenously injected ). Shortly after injection of cells, tumor cells localized to the lungs, as observed with bioluminescent imaging(Data not shown). After three days of treatment, both IgAl and IgGl cleared the tumor cells, while cells were still present in mice treated with
• Anti GD2-IgAl does not induce pain
• IgGl antibodies directed against GD2 A major limitation of IgGl antibodies directed against GD2 is the increased sensitivity to light touch after treatment. To test whether the lack of complement activation by IgA would mitigate this problem, we conducted in vivo pain experiments in mice. Paw retraction thresholds after stimulation with von Frey hairs were determined as a measure for allodynia.
• mice were intraperi tone ally injected with either a low dose of IgGl (20 ⁇ g), corresponding to a dose of 100 ⁇ g of IgA at 24 hours or a high dose of IgGl (100 ⁇ g) (Figure 18A).
• the antibody concentration found back in the serum after 24 hours is in line with the clinical phenotype after chl4.18 treatment.
• Treatment with 20 ⁇ g of IgGl chl4.18 showed a significant decrease in withdrawal threshold, which returned to baseline after 48h.
• a sub-therapeutic dose of IgGl (4 ⁇ g) did not lead to a significant reduction in withdrawal threshold.
• kits comprising compositions of engineered antibody or fragments thereof.
• kits for the reduction of a complement response such as reducing complement activation
• kits for the treatment of a cancer, pathogen infection, immune disorder or allogeneic transplant can include a therapeutic or prophylactic composition containing an effective amount of an engineered antibody in unit dosage form.
• a kit comprises a sterile container which can contain a therapeutic composition of engineered antibodies or fragments thereof; such containers can be boxes, ampules, bottles, vials, tubes, bags, pouches, blister-packs, or other suitable container forms known in the art.
• Such containers can be made of plastic, glass, laminated paper, metal foil, or other materials suitable for holding medicaments.
• engineered antibodies and fragments thereof can be provided together with instructions for administering the antibody or fragment thereof to a subject having or at risk of developing a toxicity, complement-associated toxicity, cancer, pathogen infection, immune disorder or allogeneic transplant.
• Instructions can generally include information about the use of the administration how to utilize the composition to treat toxicity, a side effect, cancer, pathogen infection, immune disorder or allogeneic transplant.
• the present invention shows that IgAl chl4.18 offers surprisingly strong anti- tumoral effects through neutrophil mediated ADCC and does not induce allodynia in vivo.
• Blocking the C5a receptor with an antagonist completely stopped allodynia (Sorkin et al 2010, Pain 149(1): 135-142).
• a first approach was to mutate the Clq-binding site of chl4.18 (K322A). This mutation is thought to abrogate complement activation of chl4.18 (Sorkin et al 2010, Pain 149(1): 135- 142). This approach only reduced complement activation, and, as a result, residual pain remained.
• IgA mediated killing was improved by the addition of GM-CSF.
• IL-2 does not seem to improve IgA or IgG mediated killing.
• IL-2 was added to the clinical regimen after effects were seen in GD2-expressing melanoma and sarcoma patients which were treated with chl4.18.
• the effects of IL-2 on neuroblastoma therapy remain unclear. This is further stressed by the clinical trial which compared immunotherapy with and without IL-2 (Ladenstein et al 2013 MAbs 5(5): 801-809). The authors show that the addition of IL-2 did not have significant effects on EFS or OS and that early termination because of toxicity was significantly higher in the IL-2 arm.
• IgAl lacks a Clq binding site, complement activation of IgA has been documented.
• the MBL pathway was shown to be activated by polymeric IgA, while the classical complement pathway was triggered for monomeric IgA directed against CD20 (Roos et al; 2001; J Immunol 167(5): 2861-2868 andLohse, Loew et al; 2018; Br J Haematol 181(3): 413-417). Nevertheless, IgAl chl4.18 did not induce CDC of neuroblastoma cell lines and did not induce complement- dependent allodynia in mice.
• mice In our studies, we dosed mice with 5 times the amount of IgA compared to IgGl to adjust for the difference in half- life between the two antibodies in mice. A similar neuronal exposure and serum concentration could be achieved with the two antibodies. For longer models, multiple doses of IgAl were injected to account for this. Although the in vivo half-life of IgA is approximately a week in humans, several approaches can be undertaken to improve the half-life of IgA in mice to improve future comparisons (Morell et al, 1973, Clin Exp Immunol 13(4): 521-528). The glycosylation of IgA can be reduced to decrease clearance by binding to the asialoglycoprotein receptor.
• variable heavy and light chain sequences of chl4.18 were derived from Biologic License Application 125516.
• the variable heavy chain sequences were cloned into Lonza expression vectors (pEE14.4), coding for the IgAl or IgGl heavy chain while the variable light chain sequences were cloned into Lonza expression vectors (pEE14.4) coding for the kappa light chain.
• Monomeric antibodies were produced by transient transfection of HEK293F cells with vectors coding for the heavy chain, light chain and pAdvantage (accession number U47294; promega), using 293Fectin transfection reagent according to the manufacturer's instructions.
• IgGl antibodies were purified using protein A columns (Hi-trap protein A) coupled to an
• AKTAprime plus chromatography system (GE lifesciences). Bound antibody was eluted with 0.1M sodium acetate pH 2.5 and neutralized with 1M TRIS-HCl pH 8.8. The eluate was dialyzed against PBS. IgAl antibodies were purified using kappa light chain affinity chromatography columns (Hi-trap kappaSelect) and eluted with 0.1M glycine buffer pH 2.5. The eluate was applied on a SEC column ran with PBS as mobile phase. The fractions containing monomeric IgA were collected and concentrated with 100 KDa spin columns. All antibodies were filtered over 0.22 ⁇ filters. Purity and stability of the antibodies was analyzed by HP-SEC (Yarra 3u SEC-2000 column) with 100 mM sodium phosphate, 150 mM NaCl pH 6.8 as mobile phase with detection at 280 nm.
• NHS-fluorescein was removed by using sephadex columns (NAP- 5, GE-healthcare), according to the manufacturer's instructions.
• Antibodies were labelled with alexa fluor-488 antibody labeling kit (ThermoFisher) according to the manufacturer's instructions.
• HEK293F cells were cultured in FreeStyle 293 expression medium at 37°C in a humidified incubator with orbital shaker platform containing 8% CO 2.
• 100.000 neuroblastoma cells were plated out in 96 well plates and centrifuged for 2 minutes at 1500 RPM. Cells were washed and incubated with fluorescein-labelled antibody at several concentrations for 45 minutes. Next, cells were centrifuged for 2 minutes at 1500 RPM, washed and resuspended in PBS. The amount of bound antibody to the cells was quantified by flow cytometry (BD FacsCanto II, BD). Cell based affinity measurements
• IMR32 neuroblastoma cells were plated out on the side of a 10 cm culture dish in an elliptical shape and incubated overnight for attaching to the plate.
• the number of leukocytes used per well corresponds to the number of leukocytes present in 50 ⁇ 1 of blood before lysis.
• blood was added on top of FicoU/Histopaque 1119 layers and centrifuged for 25 minutes at 1500 RPM without braking. Afterwards, PBMC's and PMN's were collected from the interphase between serum and ficoll or in the histopaque layer respectively.
• the effector-to-target (E:T) ratios were 80: 1 for PBMCs, 40: 1 for PMNs.
• Effector cells antibodies at various concentration, GM-CSF and IL-2 and radioactively labeled tumor cells were added to round-bottom microtiter plates (Corning Incorporated) and incubated for 37°C in a humidified incubator containing 5% CO2. Plates were centrifuged for 2 minutes at 1500 RPM and 50 ⁇ of the supernatant was transferred to lumaplates. Radioactive signal (in cpm) was quantified in a beta-gamma counter.
• pooled human serum from 8 different healthy donors was added to a concentration of 15% and incubated for 1 hour or 4 hours. Afterwards, cells were washed and stained with 7-AAD for 15 minutes. 7-AAD uptake, representing cell lysis was quantified by flow cytometry.
• mice were maintained in the animal facility of the University of Utrecht- Experiments were conducted using both male and female C57BL/6 mice (Janvier) Mice were housed in groups under a 12: 12 light dark cycle, with food and water available ad libitum. Mice were acclimatized for at least 1 week prior to the start of experiment. Sample sizes were calculated with power analysis at the time of the design of experiments.
• mice were euthanized by cervical dislocation. Mechanical thresholds were assessed for 48 hours.
• mice were injected
• mice intraperi tone ally with 5xl() 6 GD2 expressing EL4 cells expressing (ATCC). After 1 day, mice were intraperitoneally injected with 100 ⁇ g of IgGl chl4.18, or 100 ⁇ g of IgAl chl4.18. After 2 days, blood was taken and mice were injected with luciferin and subjected to bioluminescence analysis. Afterwards mice were euthanized by cervical dislocation.
• GD2 antibody binding to neurons was visualized by i.v. injection of 20 ⁇ ig or 100 ⁇ g alexa-488 labeled IgGl chl4.18 or 20 ⁇ g of IgAl chl4.18.
• Sciatic nerves were isolated and ⁇ thick slices were prepared with a cryostat cryotome and placed on slides. Slides were fixed for 10 minutes in 4% PFA and washed. Finally, slides were counterstained with DAPI, washed and treated with fluorsave. Slides were dried overnight at 4°C and images were taken by fluorescence microscopy.

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