WO2019023226A1 - Compositions et procédés permettant d'améliorer la santé des plantes et de lutter contre les maladies touchant les plantes et les organismes nuisibles pour les plantes - Google Patents

Compositions et procédés permettant d'améliorer la santé des plantes et de lutter contre les maladies touchant les plantes et les organismes nuisibles pour les plantes Download PDF

Info

Publication number
WO2019023226A1
WO2019023226A1 PCT/US2018/043479 US2018043479W WO2019023226A1 WO 2019023226 A1 WO2019023226 A1 WO 2019023226A1 US 2018043479 W US2018043479 W US 2018043479W WO 2019023226 A1 WO2019023226 A1 WO 2019023226A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
pythium
bacterial strain
active variant
phytophthora
Prior art date
Application number
PCT/US2018/043479
Other languages
English (en)
Inventor
Brooke W. BISSINGER
Billie ESPEJO
Esther Gachango
Philip E. Hammer
Chad Alton KEYSER
Mary E. KRONER
Narendra PALEKAR
Steven J. RONYAK
Mathias TWIZEYIMANA
Original Assignee
AgBiome, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AgBiome, Inc. filed Critical AgBiome, Inc.
Publication of WO2019023226A1 publication Critical patent/WO2019023226A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/075—Bacillus thuringiensis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/11—Bacillus megaterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/39—Pseudomonas fluorescens

Definitions

  • the invention relates to bacterial strains and populations for controlling plant pests and disease and/or improving an agronomic trait of interest in a plant.
  • Plant diseases and plant pests are responsible for significant agricultural losses. Effects can range from mild symptoms to catastrophic plant damage, which can lead to major economic and social consequences. Methods are needed to effectively control plant diseases and the pathogens that cause them and to effectively control plant pests.
  • compositions and methods for controlling plant diseases and plant pests and/or for improving at least one agronomic trait of interest in a plant comprise a population of biocontrol agents or bacterial strains that control one or more pathogens that cause plant disease, one or more plant pests, and/or improve at least one agronomic trait of interest.
  • the biological agents or bacterial strains can be used as an inoculant for plants.
  • Methods for growing a plant susceptible to plant disease and methods and compositions for controlling plant disease and plant pests are also provided. Further provided are methods and compositions of increasing disease resistance and pest resistance in plants. Methods and compositions for improving plant health and/or improving at least one agronomic trait of interest are also provided.
  • compositions and methods for improving at least one agronomic trait of interest and/or improving plant health and/or for controlling one or more plant diseases and/or plant pests are provided.
  • a biological agent, biocontrol agent, bacterial strain, modified bacterial strain, modified biological agent, or modified biocontrol agent or active variant thereof are used herein to describe a microorganism that is used to control plant pests, disease-causing plant pathogens and/or improve at least one agronomic trait of interest and/or improve plant health.
  • bacterial strains which can be used to control one or more plant pest, one or more plant disease, and/or improve at least one agronomic trait of interest and/or improve plant health.
  • bacterial strains include AIP011864 (a Bacillus thuringiensis strain), AIP060073 (a Bacillus amyloliquefaciens strain), AIP089963 (a Filimonas lacunae strain), AIP098363 (a Pseudomonas poae strain), AIP054629 (a Arthrobacter phenanthrenivorans strain), AIP038494 (a Bacillus megaterium strain), AIP064474 (a Bacillus thuringiensis strain), AIP085152 (a Escherichia coli strain), AIP004618 (a Escherichia coli strain), AIP037827 (a Pseudomonas fluorescens strain).
  • Cell populations comprising one or more of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827 are provided, as well as, populations of spores derived from each of these strains, or any preparation thereof.
  • various bacterial strains and/or the pesticidal compositions provided herein comprise as an active ingredient (a) a cell population comprising one or more of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • AIP011864 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67397.
  • AIP060073 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67394.
  • AIP089963 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 10, 2017 and assigned NRRL No. B- 67377.
  • AIP098363 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67390.
  • AIP054629 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67387.
  • AIP038494 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67392.
  • AIP064474 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 10, 2017 and assigned NRRL No. B- 67395.
  • AIP085152 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67385.
  • AIP004618 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67386.
  • AIP037827 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67389.
  • AIP085784 was deposited with the Patent Depository of the National Center for Agricultural Utilization Research Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604 U.S.A. on February 27, 2017 and assigned NRRL No. B- 67393.
  • Isolated encompasses a bacterium, spore, or other entity or substance, that has been (1) separated from at least some of the components with which it was associated when initially produced (whether in nature or in an experimental setting), and/or (2) produced, prepared, purified, and/or manufactured by the hand of man. Isolated bacteria may be separated from at least about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, or more of the other components with which they were initially associated.
  • a substance is “pure” if it is substantially free of other components.
  • the terms “purify,” “purifying” and “purified” refer to a bacterium, spore, or other material that has been separated from at least some of the components with which it was associated either when initially produced or generated (e.g., whether in nature or in an experimental setting), or during any time after its initial production.
  • a bacterium or spore or a bacterial population or a spore population may be considered purified if it is isolated at or after production, such as from a material or environment containing the bacterium or bacterial population or spore, and a purified bacterium or bacterial population or spore may contain other materials up to about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, or above about 90% and still be considered purified.
  • purified bacteria or spores and bacterial populations or spore populations are more than about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, or more than about 99% pure.
  • a culture of bacteria contains no other bacterial species in quantities to be detected by normal bacteriological techniques.
  • population is intended a group or collection that comprises two or more individuals (i.e., 10, 100, 1,000, 10,000, lxlO 6 , lxlO 7 , or lxlO 8 or greater) of a given bacterial strain.
  • compositions are provided herein that comprise a population of at least one bacterial strain or a mixed population of individual from more than one bacterial strain.
  • the population of at least one of a bacterial strain i.e., AIP011864, AIP060073, AIP089963, AIP098363,
  • AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof) comprises a concentration of at least about 10 s CFU/ml to about 10 n CFU/ml, about 10 s CFU/ml to about 10 10 CFU/ml, about 10 s CFU/ml to about 10 12 CFU/ml, about 10 s CFU/ml to about 10 6 CFU/ml, about 10 6 CFU/ml to about 10 7 CFU/ml, about 10 7 CFU/ml to about 10 s CFU/ml, about 10 s CFU/ml to about 10 9 CFU/ml, about 10 9 CFU/ml to about 10 10 CFU/ml, about 10 10 CFU/ml to about 10 n CFU/ml, about 10 n CFU/ml to about 10 12 CFU/ml.
  • the concentration of the bacterial strain provided herein or active variant thereof comprises at least about 10 s CFU/ml, at least about 10 6 CFU/ml, at least about 10 7 CFU/ml, at least about 10 s CFU/ml, at least about 10 9 CFU/ml, at least about 10 10 CFU/ml, at least about 10 n CFU/ml, or at least about 10 12 CFU/ml.
  • a "spore” refers to at least one dormant (at application) but viable reproductive unit of a bacterial species.
  • Non-limiting methods by which spores are formed from each of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, and AIP085784 (or variants of any thereof) are disclosed elsewhere herein.
  • populations disclosed herein can comprise a combination of vegetative cells and forepores (cells in an intermediate stage of spore formation); a combination of forespores and spores; or a combination of forespores, vegetative cells and/or spores.
  • derived from means directly isolated or obtained from a particular source or alternatively having identifying characteristics of a substance or organism isolated or obtained from a particular source.
  • source is an organism
  • derived from means that it may be isolated or obtained from the organism itself or culture broth, suspension, or medium used to culture or grow said organism.
  • a compound or composition "derived from” or “obtainable from” means that the compound or composition may be isolated from or produced by a cell culture or a whole cell broth, or suspension, filtrate, supernatant, fraction, or extract derived from a cell culture or a whole cell broth.
  • the filtrate or extract may then be diluted with another composition, such as water, buffer, fresh media, and/or a formulation.
  • another composition such as water, buffer, fresh media, and/or a formulation.
  • Such diluted filtrates or extracts are still considered filtrates and extracts of the invention.
  • “metabolite” refers to a compound, substance, or byproduct of fermentation of a bacterial strain (i.e., at least one of AIP011864, AIP060073, AIP089963, AIP098363,
  • An effective compound or metabolite is a compound present in the supernatant, whole cell broth, or bacterial strain which may improve any agronomic trait of interest of a plant, or controls a plant pest or plant pathogen that causes a plant disease, when applied to a plant of interest at an effective amount.
  • a compositions of the invention comprises a diluted filtrate, diluted extract, or diluted supernatant derived from the fermentation of a bacterial strain, wherein said composition comprises a diluted amount of the effective compound or metabolite compared to the amount whole cell broth or undiluted supernatant of said bacterial strain, wherein the bacterial is at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the diluted filtrate, diluted extract, or diluted supernatant may still comprise an effective amount of the effective compound or metabolite.
  • compositions and methods described herein comprise or are derived from a bacterial strain (i.e., at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores, from any one of
  • At least one bacterial strain is cultivated in nutrient medium using methods known in the art.
  • the bacterial strain can be cultivated by shake flask cultivation or by small scale or large scale fermentation (including but not limited to continuous, batch, fed-batch, or solid state fermentation) in laboratory or industrial fermenters performed in a suitable medium and under conditions allowing for bacterial cell growth.
  • the cultivation can take place in suitable nutrient medium comprising carbon and nitrogen sources and inorganic salts, using prodedures known in the art Suitable media are available from commercial sources or are prepared according to publications well-known in the art.
  • compounds, metabolites, and/or compositions can be extracted from the culture broth.
  • the extract can be fractionated by chromatography.
  • the extract can be further purified using methods well-known in the art.
  • the extract can also be diluted using methods well- known in the art.
  • compositions comprising or derived from a bacterial strain (i.e., at least one of
  • Active variants of the various bacterial strains provided herein include, for example, any isolate or mutant of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, or AIP085784.
  • a mutation in the swrA gene of a Bacillus spp. may result in impaired swarming ability.
  • This mutation causes the bacteria to form a more robust biofilm compared to the wild-type strain, thereby enhancing the ability of the mutated strain to improve an agronomic trait of interest of a plant or controls a plant pest or plant pathogen that causes a plant disease.
  • a similarly modified bacterial strain can be considered an active variant of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, or AIP085784.
  • An increase in resistance to a biocide refers to the ability of an organism (i.e., bacterial cell or spore) to survive and reproduce following exposure to a dose of the biocide (e.g, herbicide, fungicide, pesticide, or other crop protection chemical) that would normally be lethal to the unmodified organism or would substantially reduce growth of the unmodified organism.
  • a biocide i.e., for example, a herbicide, fungicide, pesticide, or other crop protection chemical resistance
  • compositions provided herein include selected or engineered bacterial strains and modified populations of bacterial strains. These bacterial strains or modified bacterial strains can be used as an inoculant for plants. They can also be applied as a spray application directly to the aerial parts of plants, and can be mixed with the herbicide or other chemical to which they have been modified to become tolerant.
  • the bar gene encodes resistance to the herbicide basta
  • the nptll gene encodes resistance to the antibiotics kanamycin and geneticin
  • the ALS-gene mutants encode resistance to the sulfonylurea herbicides including chlorsulfuron, metsulfuron, sulfometuron, nicosulfuron, rimsulfuron, flazasulfuron, sulfosulfuron, and triasulfuron
  • the imadizolinone herbicides including imazethapyr, imazaquin, imazapyr, and imazamethabenz.
  • Active variants of the bacteria identified by this criteria will also retain the ability to improve at least one agronomic trait when applied in an effective amount to a plant, plant part, or an area of cultivation, including for example, reducing plant disease severity and/or reducing plant disease development.
  • the active variant of the bacterial strain(s) disclosed herein include strains that are closely related to any of the disclosed strains on the basis of the Average Nucleotide Identity (ANI) method of organism classification.
  • ANI Average Nucleotide Identity
  • AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, or AIP085784 disclosed herein includes a bacterial stain that falls within at least a 90%, 95%, 96%, 97%, 97.5%, 98%, 98.5%, 98.8%, 99%, 99.5%, or 99.8% cut off score employing the A method of organism classification as set forth in Konstantinidis, K.T., et al. , (2005) PNAS USA 102(7):2567-72, which is herein incorporated by reference in its entirety.
  • AIP064474, AIP085152, AIP004618, AIP037827, or AIP085784 disclosed herein includes a bacterial stain that falls within at least a 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99.0%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or 100% cut-off score employing the ANI method of organism classification as set forth in Konstantinidis, K.T., et al. , (2005).
  • an active variant of a bacterial strain disclosed herein includes a distance metric of less than about 0.005, 0.010, 0.015, 0.020, 0.025, or 0.030.
  • a genome, as it relates to the Mash distance includes both bacterial chromosomal DNA and bacterial plasmid DNA.
  • the active variant of a bacterial strain has a genome that is above a Mash distance threshold to the disclosed strains that is greater than dissimilarity caused by technical variance.
  • the active variant of a bacterial strain has a genome that is above a Mash distance threshold to the disclosed strains that is greater than dissimilarity caused by technical variance and has a Mash distance of less than about 0.015.
  • “above technical variation” means above the Mash distance between two strains caused by errors in the genome assemblies provided the genomes being compared were each DNA sequenced with at least 20X coverage with the Illumina HiSeq 2500 DNA sequencing technology and the genomes are at least 99% complete with evidence for contamination of less than 2%. While 20X coverage is an art recognized term, for clarity, an example of 20X coverage is as follows: for a genome size of 5 megabases (MB), 100 MB of DNA sequencing from the given genome is required to have 20X sequencing coverage on average at each position along the genome. There are many suitable collections of marker genes to use for genome completeness calculations including the sets found in Campbell et al. (2013) PNAS USA 110(14):5540-45, Dupont et al.
  • Genomes of sufficient quality for comparison must be produced.
  • a genome of sufficient quality is defined as a genome assembly created with enough DNA sequence to amount to at least 20X genome coverage using Illumina HiSeq 2500 technology. The genome must be at least 99% complete with contamination of less than 2% to be compared to the claimed microbe's genome.
  • Genomes are to be compared using the Minhash workflow as demonstrated in Ondov et al. (2016) Genome Biology 17: 132, herein incorporated by reference in its entirety. Unless otherwise stated, parameters employed are as follows: “sketch” size of 1000, and "k-mer length" of 21.
  • a Mash distance of 0.015 between two genomes means the expected mutation rate is 0.015 mutations per homologous position.
  • Active variants of the bacteria identified by such methods will retain the ability to improve at least one agronomic trait when applied in an effective amount to a plant, plant part, or an area of cultivation, including for example, reducing plant disease severity, reducing plant disease development, and/or increasing pest resistance.
  • AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof) can be formulated as a cell paste, wettable powders, a cell pellet, dusts, granules, a slurry, a dry powder, aqueous or oil based liquid products, and the like.
  • Such formulations will comprise the bacteria provided herein, an active variant thereof, or a composition derived therefrom, in addition to carriers and other agents.
  • the formulations can be used in a variety of methods as disclosed elsewhere herein.
  • the bacterial strains disclosed herein, the active variants thereof, and/or a composition derived therefrom, can be formulated to include at least one or more of an extender, a solvent, spontaneity promoter, carrier, emulsifier, dispersant, frost protectant, thickener, and/or adjuvant.
  • the extender, solvent, spontaneity promoter, carrier, emulsifier, dispersant, frost protectant, thickener, and/or adjuvant is a non-natural or synthetic extender, a solvent, spontaneity promoters, carriers, emulsifiers, dispersants, frost protectants, thickeners, and/or adjuvants.
  • the formulations or application forms of the various bacterial strains, active variants thereof, and/or a composition derived therefrom can comprise, but are not limited to, auxiliaries, such as extenders, solvents, spontaneity promoters, carriers, emulsifiers, dispersants, frost protectants, biocides, solid carriers, surfactants, thickeners and/or other auxiliaries, such as adjuvants.
  • auxiliaries such as extenders, solvents, spontaneity promoters, carriers, emulsifiers, dispersants, frost protectants, biocides, solid carriers, surfactants, thickeners and/or other auxiliaries, such as adjuvants.
  • An adjuvant in this context is a component which enhances the biological effect of the formulation, without the component itself having a biological effect.
  • adjuvants are agents which promote the retention, spreading, attachment to the leaf surface, or penetration.
  • non-limiting liquid solvents are: aromatics such as xylene, toluene or alkylnaphthalenes, chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons such as cyclohexane or paraffins, for example petroleum fractions, mineral and vegetable oils, alcohols such as butanol or glycol and also their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethylformamide and dimethyl sulphoxide, and also water.
  • aromatics such as xylene, toluene or alkylnaphthalenes
  • chlorinated aromatics and chlorinated aliphatic hydrocarbons such as chlorobenzenes, chloroethylenes or methylene chloride
  • Non-limiting solvents are, for example, aromatic hydrocarbons, such as xylene, toluene or alkylnaphthalenes, for example, chlorinated aromatic or aliphatic hydrocarbons, such as chlorobenzene, chloroethylene or methylene chloride, for example, aliphatic hydrocarbons, such as cyclohexane, for example, paraffins, petroleum fractions, mineral and vegetable oils, alcohols, such as methanol, ethanol, isopropanol, butanol or glycol, for example, and also their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, for example, strongly polar solvents, such as dimethyl sulphoxide, and water.
  • aromatic hydrocarbons such as xylene, toluene or alkylnaphthalenes
  • chlorinated aromatic or aliphatic hydrocarbons such as chlor
  • Carriers suitable for granules include the following: for example, crushed and fractionated natural minerals such as calcite, marble, pumice, sepiolite, dolomite, and also synthetic granules of inorganic and organic meals, and also granules of organic material such as sawdust, paper, coconut shells, maize cobs, and tobacco stalks.
  • Liquefied gaseous extenders or solvents may also be used.
  • extenders or carriers which at standard temperature and under standard pressure are gaseous, examples being aerosol propellants, such as halogenated hydrocarbons, and also butane, propane, nitrogen and carbon dioxide.
  • emulsifiers and/or foam-formers, dispersants or wetting agents having ionic or nonionic properties, or mixtures of these surface-active substances are salts of polyacrylic acid, salts of lignosulphonic acid, salts of phenolsulphonic acid or naphthalenesulphonic acid, polycondensates of ethylene oxide with fatty alcohols or with fatty acids or with fatty amines, with substituted phenols (preferably alkylphenols or arylphenols), salts of sulphosuccinic esters, taurine derivatives (preferably alkylta urates), phosphoric esters of polyethoxylated alcohols or phenols, fatty acid esters of polyols, and derivatives of the compounds containing sulphates, sulphonates and phosphates, examples being alkylaryl polyglycol ethers, alkylsulphonates, alkyl sulphates, arylsulphonates, protein hydrolys,
  • auxiliaries that may be present in the formulations and in the application forms derived from them include colorants such as inorganic pigments, examples being iron oxide, titanium oxide, Prussian Blue, and organic dyes, such as alizarin dyes, azo dyes and metal phthalocyanine dyes, and nutrients and trace nutrients, such as salts of iron, manganese, boron, copper, cobalt, molybdenum, and zinc.
  • colorants such as inorganic pigments, examples being iron oxide, titanium oxide, Prussian Blue, and organic dyes, such as alizarin dyes, azo dyes and metal phthalocyanine dyes, and nutrients and trace nutrients, such as salts of iron, manganese, boron, copper, cobalt, molybdenum, and zinc.
  • Stabilizers such as low-temperature stabilizers, preservatives, antioxidants, light stabilizers or other agents which improve chemical and/or physical stability may also be present. Additionally present may be foam-formers or defoamers.
  • the formulations and application forms derived from them may also comprise, as additional auxiliaries, stickers such as carboxymethylcellulose, natural and synthetic polymers in powder, granule or latex form, such as gum arabic, polyvinyl alcohol, polyvinyl acetate, and also natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids.
  • additional auxiliaries include mineral and vegetable oils.
  • auxiliaries include fragrances, protective colloids, binders, adhesives, thickeners, thixotropic substances, penetrants, retention promoters, stabilizers,
  • the active compounds may be combined with any solid or liquid additive commonly used for formulation purposes.
  • Suitable penetrants in the present context include all those substances which are typically used in order to enhance the penetration of active agrochemical compounds into plants.
  • Penetrants in this context are defined in that, from the (generally aqueous) application liquor and/or from the spray coating, they are able to penetrate the cuticle of the plant and thereby increase the mobility of the active compounds in the cuticle. This property can be determined using the method described in the literature (Baur et al., 1997, Pesticide Science 51 : 131-152).
  • compositions and formulations disclosed herein can comprise an amount of a bacterial strain, such as AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores, and/or a composition derived from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • a bacterial strain such as AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP0384
  • Such an amount can comprise a concentration of the bacterial strain of at least about 10 4 to about 10 n , at least about 10 s CFU/gram to about 10 n CFU/gram, about 10 s CFU/gram to about 10 10 CFU/gram, about 10 s CFU/gram to about 10 12 CFU/gram, about 10 s CFU/gram to about 10 6 CFU/gram, about 10 6 CFU/gram to about 10 7 CFU/gram, about 10 7 CFU/gram to about 10 s CFU/gram, about 10 s
  • the concentration of the bacterial strain comprises at least about 10 s CFU/gram, at least about 10 6 CFU/gram, at least about 10 7 CFU/gram, at least about 10 s CFU/gram, at least about 10 9 CFU/gram, at least about 10 10 CFU/gram, at least about 10 n CFU/gram, at least about 10 12 CFU/ gram.
  • the concentration of the bacterial strain comprises at least about 10 s CFU/gram, at least about 10 6 CFU/gram, at least about 10 7 CFU/gram, at least about 10 s CFU/gram, at least about 10 9 CFU/gram, at least about 10 10 CFU/gram, at least about 10 n CFU/gram, at least about 10 12
  • the amount of the bacterial strain can comprise a concentration of the bacterial strain of at least about 10 s CFU/gram to about 10 n CFU/gram, about 10 7 CFU/gram to about 10 10 CFU/gram, about 10 7 CFU/gram to about 10 n CFU/gram, about 10 6 CFU/gram to about 10 10 CFU/gram, about 10 6 CFU/gram to about 10 n CFU/gram, about 10 n CFU/gram to about 10 12 CFU/gram, about 10 s CFU/gram to about 10 10 CFU/gram, about 10 s CFU/gram to about 10 12
  • a "cell paste” comprises a population of cells that has been centrifuged and/or filtered or otherwise concentrated.
  • composition comprising a supernatant, filtrate, or extract derived from at least one of bacterial strain AIP011864, AIP060073, AIP089963, AIP098363, AIP054629,
  • the composition contains effective compound(s) and/or metabolite(s) which improve an agronomic trait of interest of a plant or controls a plant pest or plant pathogen that causes disease.
  • the supernatant refers to the liquid remaining when cells are grown in broth or are harvested in another liquid from an agar plate and are removed by centrifugation, filtration, sedimentation, or other means well known in the art. The supernatant may be further concentrated to produce a filtrate.
  • the filtrate may comprise a
  • a coated seed which comprises a seed and a coating on the seed, wherein the coating comprises at least one bacterial strain, such as AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore or a forespore or a combination of cells, forespores or/and spores, and/or a composition derived from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, wherein said bacterial strain or the active variant thereof is present on the seed at about 10 s CFU/seed to about 10 7
  • a seed coating can further comprise at least at least one nutrient, at least one herbicide or at least one pesticide, or at least one biocide. See, for example, US App Pub. 20040336049,
  • the various formulations disclosed herein can be stable for at least 30, 40, 50, 60, 70, 80, 90, 100, 125, 150, 200, 225, 250, 275, 300, 325, 350 days, 1.5 years, 2 years or longer.
  • stable is intended that the formulation retains viable bacteria, and/or retains an effective amount of a biologically active bacterial population.
  • a stable formulation may also retain an effective amount of a biologically active compound or metabolite derived from the supernatant, filtrate, or extract of a whole cell culture of a bacterial strain.
  • Biological activity as used herein refers to the ability of the formulation to improve an agronomic trait of interest or control a plant pest or plant pathogen that causes a plant disease.
  • the stable formulation retains at least about 1%, about 10%, about 20%, about 30% about 40%, about 50%, about 60%, about 70%, about 80%, or about 90% of CFU/gram in the formulation at a given storage time point when compared to the CFU/gram produced after immediate preparation of the formulation.
  • the stable formulation retains at least about 30% to 80%, about 50% to about 80%, about 60% to about 70%, about 70% to about 80%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70% of biological activity in the formulation at a given storage time point when compared to the biological activity found in the formulation immediately after production.
  • the stable formulation at a given storage time point retains at least about 30%, 45%, 50%, 60%, 70%, 80%, 90% of biological activity when compared to the biological activity found in the formulation immediately after production.
  • the stable formation retains any combination of the viability and biological activity noted above.
  • the formulations preferably comprise between 0.00000001 % and 98% by weight of active compound or, with particular preference, between 0.01 % and 95% by weight of active compound, more preferably between 0.5% and 90% by weight of active compound, based on the weight of the formulation.
  • the active compound content of the application forms prepared from the formulations may vary within wide ranges.
  • the active compound concentration of the application forms may be situated typically between 0.00000001 % and 95% by weight of active compound, preferably between 0.00001 % and 1 % by weight, based on the weight of the application form.
  • Application takes place in a customary manner adapted to the application forms.
  • the bacterial strain provided herein and/or a composition derived therefrom can be used to significantly improve at least one agronomic trait of interest (i.e, reduce disease such as ASR or another fungal pathogen of interest or reduce susceptibility to plant pests, such as insect and nematode pests).
  • the bacterial strain provided herein and/or a composition derived therefrom can be used with other pesticides for an effective integrated pest management program.
  • the biocontrol populations at least one of the bacterial strains provided herein and/or a composition derived therefrom
  • Glyceryl dimyristate [Tetradecanoic acid, diester with 1,2,3-propanetriol] ;
  • Glyceryl dioleate [9- Octadecenoic acid (9Z)-, diester with 1,2,3-propanetriol];
  • Glyceryl distearate [9- Octadecenoic acid (9Z)-, diester with 1,2,3-propanetriol];
  • Glyceryl distearate Glyceryl
  • the bacterial strains or modified bacterial strains or active variants thereof provided herein and/or a composition derived therefrom can be employed with any plant species to improve an agronomic trait of interest.
  • Agronomic traits of interest include any trait that improves plant health or commercial value.
  • Non-limiting examples of agronomic traits of interest including increase in biomass, increase in drought tolerance, thermal tolerance, herbicide tolerance, drought resistance, insect resistance, fungus resistance, virus resistance, bacteria resistance, male sterility, cold tolerance, salt tolerance, increased yield, enhanced nutrient use efficiency, increased nitrogen use efficiency, increased tolerance to nitrogen stress, increased fermentable carbohydrate content, reduced lignin content, increased antioxidant content, enhanced water use efficiency, increased vigor, increased germination efficiency, earlier or increased flowering, increased biomass, altered root-to-shoot biomass ratio, enhanced soil water retention, or a combination thereof.
  • the agronomic trait of interest includes an altered oil content, altered protein content, altered seed carbohydrate composition, altered seed oil composition, and altered seed protein composition, chemical tolerance, cold tolerance, delayed senescence, disease resistance, drought tolerance, ear weight, growth improvement, health enhancement, heat tolerance, herbicide tolerance, herbivore resistance, improved nitrogen fixation, improved nitrogen utilization, improved root architecture, improved water use efficiency, increased biomass, increased root length, increased seed weight, increased shoot length, increased yield, increased yield under water-limited conditions, kernel mass, kernel moisture content, metal tolerance, number of ears, number of kernels per ear, number of pods, nutrition enhancement, pathogen resistance, pest resistance, photosynthetic capability improvement, salinity tolerance, stay-green, vigor improvement, increased dry weight of mature seeds, increased fresh weight of mature seeds, increased number of mature seeds per plant, increased chlorophyll content, increased number of pods per plant, increased length of pods per plant, reduced number of wilted leaves per plant, reduced number of severely wilted leaves per plant, and increased number
  • the bacterial strain or active variant thereof provided herein and/or a composition derived therefrom can be employed to decrease or reduce the level of a plant pest.
  • pests includes but is not limited to, insects, fungi, bacteria, nematodes, acarids, protozoan pathogens, animal-parasitic liver flukes, and the like.
  • the bacterial strain or active variant thereof provided herein and/or a composition derived therefrom can be employed with any plant species susceptible to a plant disease.
  • a plant susceptible to a plant disease is meant that the causative pathogen(s) of the plant disease are able to infect the plant.
  • a plant susceptible to a plant disease can be susceptible to a plant disease caused by an insect or nematode pest as disclosed elsewhere herein.
  • Vegetables include tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo).
  • tomatoes Locopersicon esculentum
  • lettuce e.g., Lactuca sativa
  • green beans Phaseolus vulgaris
  • lima beans Phaseolus limensis
  • peas Lathyrus spp.
  • members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo).
  • Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine ⁇ Pinus contorta), and Monterey pine ⁇ Pinus radiata); Douglas-fir ⁇ Pseudotsuga menziesii); Western hemlock ⁇ Tsuga canadensis); Sitka spruce ⁇ Picea glauca); redwood ⁇ Sequoia sempervirens); true firs such as silver fir ⁇ Abies amabilis) and balsam fir ⁇ Abies balsamea); and cedars such as Western red cedar ⁇ Thuja plicata) and Alaska yellow-cedar ⁇ Chamaecyparis nootkatensis).
  • pines such as loblolly pine (Pinus ta
  • plants of the present invention are crop plants (for example, corn, alfalfa, sunflower, Brassica, soybean, cotton, safflower, peanut, sorghum, wheat, millet, tobacco, etc.).
  • corn and soybean plants are optimal, and in yet other embodiments corn plants are optimal.
  • plants of interest include grain plants that provide seeds of interest, oil-seed plants, and leguminous plants.
  • Seeds of interest include grain seeds, such as corn, wheat, barley, rice, sorghum, rye, etc.
  • Oil-seed plants include cotton, soybean, safflower, sunflower, Brassica, maize, alfalfa, palm, coconut, etc.
  • Leguminous plants include beans, peas, and dry pulses. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc.
  • the bacterial strains provided herein are those that target one or more plant pests.
  • pests includes but is not limited to, insects, fungi, bacteria, nematodes, viruses or viroids, oomycetes, protozoa, and the like.
  • insects or "insect pests” as used herein refers to insects and other similar pests.
  • the term “insect” encompasses eggs, larvae, juvenile and mature forms of insects. Insects can be targeted at any stage of development. For example, insects can be targeted after the first instar, during the second instar, third instar, fourth instar, fifth instar, or any other developmental or adult growth stage.
  • instar is used to denote the developmental stage of the larval or nymphal forms of insects. Insect pests include insects selected from the orders Coleoptera,
  • Lepidoptera Hemiptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Trombidiformes, Dermaptera, Isoptera, Anoplura, Siphonaptera,
  • Insect pests of the order Coleoptera include, but are not limited to, Agriotes spp., Anthonomus spp., Atomaria linearis, Chaetocnema tibialis, Cosmopolites spp., Curculio spp., Dermestes spp., Epilachna spp., Eremnus spp., Leptinotarsa decemlineata, Lissorhoptrus spp., Melolontha spp., Orycaephilus spp., Otiorhynchus spp., Phlyctinus spp., Popillia spp., Psylliodes spp., Rhizopertha spp., Scarabeidae, Sitophilus spp., Sitotroga spp., Tenebrio spp., Tribolium spp.
  • Coleoptera insects include, but are not limited to weevils from the families Anthribidae, Bruchidae, and Curculionidae (e.g., sweetpotato weevil ⁇ Cylas formicarius (Fabricius)), boll weevil ⁇ Anthonomus grandis Boheman), rice water weevil ⁇ Lissorhoptrus oryzophilus Kuschel), rice weevil (Sitophilus oryzae L.)), and also flea beetles, cucumber beetles, rootworms, leaf beetles, potato beetles, and leafminers in the family Chrysomelidae (e.g., Colorado potato beetle ⁇ Leptinotarsa decemlineata Say), western corn rootworm (Diabrotica virgifera virgifera LeConte)); chafers and other beetles from the family Scarib
  • Insect pests of the order Lepidoptera include, but are not limited to, Achoroia grisella, Acleris gloverana, Acleris variana, Adoxophyes orana, Agrotis ipsilon, Alabama argillacea, Alsophila pometaria, Amyelois transitella, Anagasta kuehniella, Anarsia lineatella, Anisota senatoria,
  • Antheraea pernyi Anticarsia gemmatalis, Archips spp., Argyrotaenia spp., Athetis mindara, Bombyx mori, Bucculatrix thurberiella, Cadra cautella, Choristoneura spp., Cochylls hosppes, Colias eurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella, Datana integerrima, Dendrolimus sibericus, Desmiafeneralis, Diaphania hyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraea saccharalis, Ennomos subsignaria, Eoreuma loftini, Espphestia elutella, Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoellia ambiguella
  • Insect pests of the order Hemiptera include, but are not limited to, Lygus spp., including
  • Insect pests of the order Hemiptera also include Eurygaster ssp. , Coreidae ssp., Pyrrhocoridae ssp., Blostomatidae ssp., Reduviidae ssp., Cimicidae ssp. ; Aleurocanthus woglumi, citrus blackfly; Aleyrodes proletella, cabbage whitefly; Bemisia argentifolii, silverleaf whitefly; Trialeurodes vaporariorum, greenhouse whitefly, and Bemisia tabaci, sweet potato whitefly.
  • Insect pests of the order Thysenoptera include, but are not limited to, thrips species, including Frankliniella spp., for example Western Flower thrips (Frankliniella occidentalis (Pergande)); Thrips spp., for example Thrips tabaci; Scirtothrips spp., for example Scirtothrips dorsalis; Klambothrips spp., for example Klambothrips myopori; Echinothrips spp., for example Echinothrips americanus; and Megalurothrips spp., for example Megalurothrips usitatus.
  • thrips species including Frankliniella spp., for example Western Flower thrips (Frankliniella occidentalis (Pergande)); Thrips spp., for example Thrips tabaci; Scirtothrips spp., for example Scirtothrips dorsalis; Klambothrips
  • Insect pests of the order Trombidiformes include, but are not limited to, plant feeding mites, including six-spooted spider mite (Eutetranychus sexmaculatus), Texas citrus mite (Eutetranychus banksi), Citrus red mite (Panonychus citri), European red mite (Panonychus ulmi), McDaniel mite (Tetranychus mcdanieli), Pacific spider mite (Tetranychus pacificus), Strawberry spider mite (Tetranychus urticae), Spruce spider mite ⁇ Oligonychus ununguis), Sugi spider mite ⁇ Oligonychus nondonensisi), and Tetranychus evansi.
  • six-spooted spider mite Eutetranychus sexmaculatus
  • Texas citrus mite Eutetranychus banksi
  • Citrus red mite Panonychus citri
  • European red mite Panonych
  • Insect pests of the order Acari including, but not limited to, mites and ticks.
  • Insect pests of interest include insect pests of crops of high agronomic importance. These insect pests include, but are not limited to, insect pests for the following crops: Maize: Ostrinia nubilalis, European corn borer; Agrotis ipsilon, black cutworm; Helicoverpa zeae, corn earworm; Spodoptera frugiperda, fall armyworm; Diatraea grandiosella, southwestern corn borer;
  • Macrosiphum avenae English grain aphid
  • Melanoplus femurrubrum redlegged grasshopper
  • sunflower moth Zygogramma exclamationis, sunflower beetle; Bothyrus gibbosus, carrot beetle; Neolasioptera murtfeldtiana, sunflower seed midge; Cotton: Heliothis virescens, tobacco budworm; Helicoverpa zea, cotton bollworm; Spodoptera exigua, beet armyworm; Pectinophora gossypiella, pink bollworm; boll weevil, e.g., Anthonomus grandis; Aphis gossypii, cotton aphid;
  • Acrosternum hilare green stink bug
  • Melanoplus femurrubrum redlegged grasshopper
  • Melanoplus differentialis differential grasshopper
  • Hylemya platura seedcorn maggot
  • Sericothrips variabilis soybean thrips
  • Thrips tabaci onion thrips
  • Tetranychus turkestani strawberry spider mite
  • Insect pests of interest also include Araecerus fasciculatus, coffee bean weevil;
  • Pests of interest also include nematodes.
  • Nematodes include parasitic nematodes such as root-knot, cyst, and lesion nematodes, including Heterodera spp., Meloidogyne spp., and Globodera spp.; particularly members of the cyst nematodes, including, but not limited to, Heterodera glycines (soybean cyst nematode); Heterodera schachtii (beet cyst nematode); Heterodera avenae (cereal cyst nematode); and Globodera rostochiensis and Globodera pallida (potato cyst nematodes).
  • Lesion nematodes include Pratylenchus spp.
  • Bacterial strains or active variants thereof and/or a composition derived therefrom can be tested for pesticidal activity against a pest in any developmental stage, including early developmental stages, e.g., as larvae or other immature forms.
  • larvae of insect pests may be reared in total darkness at from about 20 ⁇ to about 30 ⁇ and from about 30% to about 70% relative humidity.
  • Bioassays may be performed as described in Czapla and Lang (1990) /. Econ. Entomol. 83 (6): 2480- 2485. Methods of rearing insect larvae and performing bioassays are well known to one of ordinary skill in the art.
  • the bacterial strains provided herein are those that target one or more insect or insect pests.
  • the various bacterial strains provided herein target one or more insect pests that cause damage to plants.
  • any of the bacterial strain provided herein or active variant thereof can have insecticidal activity against one, two, three, four, five, or more insect pests described herein.
  • the bacterial strains AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP089963, AIP085784 or an active variant thereof, and/or a composition derived therefrom can have activity against Western corn rootworm, Colorado potato beetle, sweet potato weevil, Southern green stink bug, Lygus spp., white flies, Bemisia spp., Myzus persicae, Thrips spp., spider mites, aphids, and/or Drosophila suzukii.
  • AIP060073 and AIP089963, or an active variant thereof have activity against Southern green stink bug.
  • AIP098363, AIP054629, AIP038494, or an active variant thereof have activity against Western corn rootworm, Colorado potato beetle, and/or sweet potato weevil.
  • AIP060073, AIP 089963, AIP089963, AIP085784, or an active variant thereof have activity against Lygus spp., white flies, Bemisia spp., Myzus persicae, Thrips spp., thrips, spider mites, aphids, and/or Drosophila suzukii.
  • plant diseases include, but are not limited to, Asian Soybean Rust (ASR), gray mold, leaf spot, Frogeye Leaf Spot, Early Blight, Damping off complex, Brown Patch, black scurf, root rot, belly rot, sheath blight, Powdery Mildew, Anthracnose leaf spot, Downy Mildew, Pythium Blight, Late Blight, Fusarium Head Blight, sudden death syndrome (SDS), Fusarium Wilt, Corn Stalk Rot, Brown Rust, Black Rust, Yellow Rust, Wheat Rust, Rust, Apple Scab, Verticillium Wilt, Fire Blight, and Brown Rot, to name a few.
  • ASR Asian Soybean Rust
  • gray mold leaf spot
  • Frogeye Leaf Spot Early Blight
  • Damping off complex Brown Patch
  • Brown Patch black scurf
  • root rot belly rot
  • sheath blight Powdery Mildew
  • Anthracnose leaf spot Downy Mildew
  • the bacterial strains provided herein are those that target one or more plant pathogens.
  • the various bacterial strains provided herein target one or more fungal pathogens that cause plant disease.
  • any of the bacterial strain provided herein or active variant thereof can have antifungal activity against one, two, three, four, five, or more fungal pathogens and/or fungal diseases described herein.
  • the fungal pathogen is selected from the group consisting of Botrytis cinerea, Cercospora sojina, Alternaria solani, Rhizoctonia solani, Erysiphe necator, Podosphaera xanthii, Colletotrichum cereal, Plasmopara viticola, Peronospora belbahrii, Pythium
  • the fungal pathogen is Phakopsora sp., including Phakopsora pachyrhizi and/or Phakopsora meibomiae.
  • the bacterial strains, active variants thereof i.e., AIP011864,
  • the bacterial strain or active variant thereof i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof), and/or a composition derived therefrom, controls at least one, two, three, four, five, or more fungal diseases selected from the group consisting of Asian Soybean Rust, gray mold, Frogeye Leaf
  • the bacterial strains or active variants thereof i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof), and/or a composition derived therefrom control at least one, two, three, four, five, or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora soj
  • the bacterial strains or active variants thereof i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores, from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof), and/or a composition derived therefrom control at least one, two, or all of Phakopsora.
  • the bacterial strain or modified biological agents disclosed herein i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of
  • the bacterial strains or modified bacterial strains disclosed herein i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073,
  • kits for treating or preventing a plant disease comprising applying to a plant having a plant disease or at risk of developing a plant disease an effective amount of at least one bacterial strain provided herein or an active variant thereof wherein the bacterial strain controls a plant pathogen that causes the plant disease.
  • methods of reducing susceptibility to a plant pest and/or increasing resistance to a plant pest comprising applying to a plant having a plant disease or at risk of developing a plant disease caused by a plant pest an effective amount of at least one bacterial strain provided herein or an active variant thereof and/or a composition derived therefrom, wherein the bacterial strain and/or the composition derived therefrom controls the plant pest, such as the insect pest.
  • the effective compound may be a composition derived from at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the composition derived from a bacterial strain described herein is extracted from the culture broth.
  • the bacterial strain provided herein or active variant thereof may comprise at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363,
  • the effective amount of the bacterial strain or active variant thereof comprises at least about 10 12 to 10 16 CFU per hectare or least about 10 4 to 10 16 CFU per hectare, or least about 10 s to 10 n CFU per hectare.
  • the bacterial strain provided herein or an active variant thereof is an antipathogenic agent that treats or prevents one, two, three, four, five or more plant diseases or infections by plant pests.
  • the bacterial strain provided herein or an active variant thereof and/or composition derived therefrom is an antifungal agent that treats or prevents one, two, three, four, five or more fungal plant diseases or infections caused by insect pests or nematode pests.
  • the bacterial strain provided herein or an active variant thereof and/or composition derived therefrom can be employed with any plant species susceptible to a plant disease of interest and/or susceptible to a plant pest of interest. Examples of diseases causes by the fungal pathogens described herein are provided in Table 1.
  • a bacterial strain provided herein or active variant thereof that controls one or more of Colletotrichum cereal, Apiognomonia errabunda, Apiognomonia veneta, Colletotrichum gloeosporiodes, Discula fraxinea can be applied to a plant having Anthracnose leaf spot or at risk of developing Anthracnose leaf spot in order to treat or prevent Anthracnose leaf spot in the plant.
  • ASR Asian Soybean Rust
  • methods of treating or preventing Asian Soybean Rust comprising applying to a plant having ASR or at risk of developing ASR an effective amount of at least one bacterial strain provided herein or an active variant thereof comprising AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, and/or a composition derived
  • the effective amount of the bacterial strain provided herein or an active variant thereof comprises at least about 10 12 to 10 16 CFU per hectare and wherein the bacterial strain provided herein or active variant thereof controls a plant pathogen that causes ASR.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is used as a foliar application on a plant to treat or prevent ASR.
  • the bacterial strain provided herein or an active variant thereof or modified bacterial strain provided herein can be employed with any plant species susceptible to ASR.
  • ASR Asian Soybean Rust
  • soybean Glycine max
  • common bean Phaseolus vulgaris
  • lima beans Phaseolus limensis
  • butter beans Phaseolus lunatus
  • cowpeas Vigna unguiculata
  • pigeon peas Cajanus cajan
  • yam beans such as jicama (Pachyrhizus erosus).
  • a soybean plant is employed.
  • the bacterial strain provided herein or an active variant thereof controls one or more fungi that causes ASR (such as, for example, Phakopsora).
  • ASR is caused by one or more fungal pathogens of the genus Phakopsora.
  • the fungal pathogens that cause ASR are Phakopsora pachyrhizi or
  • Phakopsora meibomiae The ASR pathogen is well adapted for long-distance dispersal, because the spores can be readily carried by the wind, making it an ideal means for introduction to new, rust-free regions.
  • the primary means of dissemination are spores, which can be carried by wind or splashed rain. These pathogens are obligate pathogens surviving and reproducing only on live hosts.
  • the first symptoms are light-brown polygonal lesions of 2 to 5 mm on the adaxial leaf surface. These lesions develop into volcano-shaped lesions known as pustules that appear on the abaxial surface of the leaf, where uredospores are produced.
  • the bacterial strain provided herein or an active variant thereof and/or a composition derived therefrom controls Phakopsora pachyrhizi. In yet further embodiments, the bacterial strain provided herein or active variant thereof and/or a composition derived therefrom controls Phakopsora meibomiae. Various assays to measure such activity are disclosed elsewhere herein.
  • treat or “treating” or its derivatives includes substantially inhibiting, slowing, or reversing the progression of a condition, substantially ameliorating symptoms of a condition or substantially preventing the appearance of symptoms or conditions brought about by the pathogen or pest that causes the plant disease.
  • controlling and "protecting a plant from a pathogen” refers to one or more of inhibiting or reducing the growth, germination, reproduction, and/or proliferation of a pathogen or pest of interest; and/or killing, removing, destroying, or otherwise diminishing the occurrence, and/or activity of a pathogen or pest of interest.
  • a plant treated with the bacterial strain provided herein may show a reduced disease severity or reduced disease development in the presence of plant pathogens and/or plant pests by a statistically significant amount.
  • prevent means the countering in advance of bacterial, fungal, viral, insect or other pest growth, proliferation, infestation, spore germination, and hyphae growth.
  • the composition is applied before exposure to the pathogens or plant pests.
  • ameliorate and “amelioration” relate to the improvement in the treated plant condition brought about by the compositions and methods provided herein.
  • the improvement can be manifested in the forms of a decrease in pathogen or pest growth and/or an improvement in the diseased plant height, weight, number of leaves, root system, or yield.
  • the term refers to the improvement in a diseased plant physiological state.
  • inhibitor and all variations of this term is intended to encompass the restriction or prohibition of bacterial, fungal, viral, nematode, insect, or any other pest growth, as well as spore germination.
  • the term "eliminate” relates to the substantial eradication or removal of bacteria, fungi, viruses, nematodes, insects, or any other pests by contacting them with the composition of the invention, optionally, according to the methods of the invention described below.
  • delay means the slowing of the progress of bacterial, fungal, viral, nematode, insect, or any other pest growth, and spore germination.
  • the expression “delaying the onset” is interpreted as preventing or slowing the progression of bacterial, fungal, viral, nematodes, insect, or any other pest growth, infestation, infection, spore germination and hyphae growth for a period of time, such that said bacterial, fungal, viral, nematode, insect, or any other pest growth, infestation, infection, spore germination and hyphae growth do not progress as far along in development, or appear later than in the absence of the treatment according to the invention.
  • a plant, plant part, or area of cultivation treated with the bacterial strain provided herein or an active variant thereof may show a reduced disease severity or reduced disease development in the presence of plant pathogens or plant pest by a statistically significant amount.
  • a reduced disease severity or reduced disease development can be a reduction of about 10% to about 20%, about 20% to about 30%, about 30% to about 40%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70%, about 70% to about 80%, about 80% to about 90%, or about 90% to about 100% when compared to non-treated control plants.
  • the plant treated with a bacterial strain provided herein or an active variant thereof may show a reduced disease severity or reduced disease development in the presence of plant pathogen of at least about 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%
  • a plant, plant part, or area of cultivation treated with the bacterial strain provided herein or an active variant thereof may show a reduction of plant pests, includes insect and/or nematode pests.
  • a reduction of plant pests can be a reduction of about 10% to about 20%, about 20% to about 30%, about 30% to about 40%, about 40% to about 50%, about 50% to about 60%, about 60% to about 70%, about 70% to about 80%, about 80% to about 90%, or about 90% to about 100% when compared to non-treated control plants.
  • the plant treated with a bacterial strain provided herein or an active variant thereof may show a reduction of plant pests of at least about 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%
  • Methods for measuring the number of plant pests include, counting the number of pests, or contacting plants with one or more pests and determining the plant's ability to survive and/or cause the death of the pests. See, for example, Czapla and Lang, (1990) J. Econ. Entomol. 83:2480- 2485; Andrews, et al., (1988) Biochem. J. 252: 199-206; Marrone, et al., (1985) J. of Economic Entomology 78:290-293 and U.S. Pat. No. 5,743,477, all of which are herein incorporated by reference in their entirety.
  • the insecticidal activity is against a Lepidopteran species. In one embodiment, the insecticidal activity is against a Coleopteran species. In one embodiment, the insecticidal activity is against a Hemipteran species. In some embodiments, the insecticidal activity is against one or more insect pests, such as the Western corn rootworm, the Colorado potato beetle, the sweet potato weevil, or the Southern green stink bug. In some embodiments, the insecticidal activity is against a Lygus species, such as Lygus hesperus, Lygus lineolaris, Lygus pratensis, Lygus rugulipennis Popp, and/or Lygus pabulinus.
  • Lygus species such as Lygus hesperus, Lygus lineolaris, Lygus pratensis, Lygus rugulipennis Popp, and/or Lygus pabulinus.
  • the insecticidal activity is against a stinkbug, such as Euschistus servus, Nezara viridula, Acrosternum hilare, Euschistus servus, Euschistus heros, Dichelops melacantus, Dichelops furcatus, and/or Halyomorpha halys.
  • the insecticidal activity is against an aphid, such as Lipaphis erysimi, Aphis gossypii, Macrosiphum avenae, Myzus persicae, Acyrthosiphon pisum and/or Aphidoidea ssp.
  • the insecticidal activity is against a whitefly and/or a blackfly, such as Aleurocanthus woglumi, citrus blackfly; Aleyrodes proletella, cabbage whitefly; Bemisia argentifolii, silverleaf whitefly; Trialeurodes vaporariorum, greenhouse whitefly, and Bemisia tabaci, sweet potato whitefly.
  • a whitefly and/or a blackfly such as Aleurocanthus woglumi, citrus blackfly; Aleyrodes proletella, cabbage whitefly; Bemisia argentifolii, silverleaf whitefly; Trialeurodes vaporariorum, greenhouse whitefly, and Bemisia tabaci, sweet potato whitefly.
  • the insecticidal activity is against an insect pest of the order
  • Thysenoptera such as for example thrips species, including Frankliniella spp., for example Western Flower thrips ⁇ Frankliniella occidentalis (Pergande)); Thrips spp., for example Thrips tabaci;
  • Scirtothrips spp. for example Scirtothrips dorsalis; Klambothrips spp., for example Klambothrips myopori; Echinothrips spp., for example Echinothrips americanus; and/or Megalurothrips spp., for example Megalurothrips usitatus.
  • the insecticidal activity is against an insect pest of the order
  • Trombidiformes such as a six-spoted spider mite (Eutetranychus sexmaculatus), Texas citrus mite (Eutetranychus banksi), Citrus red mite (Panonychus citri), European red mite (Panonychus ulmi), McDaniel mite (Tetranychus mcdanieli), Pacific spider mite (Tetranychus pacificus), Strawberry spider mite ⁇ Tetranychus urticae), Spruce spider mite (Oligonychus ununguis), Sugi spider mite (Oligonychus nondonensisi), and/or Tetranychus evansi.
  • a six-spoted spider mite Eutetranychus sexmaculatus
  • Texas citrus mite Eutetranychus banksi
  • Citrus red mite Panonychus citri
  • European red mite Panonychus ulmi
  • McDaniel mite Tetranychus
  • an antipathogenic composition or “antipathogenic” is intended that the compositions are capable of suppressing, controlling, preventing and/or killing the invading pathogenic organism or pest.
  • an antipathogenic composition reduces the disease symptoms resulting from pathogen challenge by a statistically significant amount, including for example, at least about 10% to at least about 20%, at least about 20% to about 50%, at least about 10% to about 60%, at least about 30% to about 70%, at least about 40% to about 80%, or at least about 50% to about 90% or greater.
  • the methods of the invention can be utilized to protect plants from disease, particularly those diseases that are caused by plant pathogens or plant pests.
  • Assays that measure antipathogenic activity are commonly known in the art, as are methods to quantitate disease resistance in plants following pathogen infection. See, for example, U.S. Patent No. 5,614,395, herein incorporated by reference. Such techniques include, measuring over time, the average lesion diameter, the pathogen biomass, and the overall percentage of decayed plant tissues. For example, a plant either expressing an antipathogenic polypeptide or having an antipathogenic composition applied to its surface shows a decrease in tissue necrosis ⁇ i.e., lesion diameter) or a decrease in plant death following pathogen challenge when compared to a control plant that was not exposed to the antipathogenic composition. Alternatively, antipathogenic activity can be measured by a decrease in pathogen biomass.
  • a plant expressing an antipathogenic polypeptide or exposed to an antipathogenic composition is challenged with a pathogen of interest.
  • tissue samples from the pathogen-inoculated tissues are obtained and RNA is extracted.
  • the percent of a specific pathogen RNA transcript relative to the level of a plant specific transcript allows the level of pathogen biomass to be determined.
  • in vitro antipathogenic assays include, for example, the addition of varying concentrations of the antipathogenic composition to paper disks and placing the disks on agar containing a suspension of the pathogen of interest. Following incubation, clear inhibition zones develop around the discs that contain an effective concentration of the antipathogenic polypeptide (Liu et al. (1994) Plant Biology 91 : 1888-1892, herein incorporated by reference). Additionally, microspectrophotometrical analysis can be used to measure the in vitro antipathogenic properties of a composition (Hu et al. (1997) Plant Mol. Biol. 34:949-959 and Cammue et al. (1992) /. Biol. Chem. 267: 2228-2233, both of which are herein incorporated by reference).
  • compositions and methods for inducing disease resistance in a plant to plant pathogens are also provided. Accordingly, the compositions and methods are also useful in protecting plants against fungal pathogens, viruses, nematodes, and insects.
  • methods of inducing disease resistance against a plant pathogen comprising applying to a plant that is susceptible to a plant disease caused by the plant pathogen an effective amount of at least one bacterial strain provided herein or active variant thereof.
  • methods of inducing disease resistance against a plant pest or inducing resistance to infection by a plant pest comprising applying to a plant that is susceptible to a plant disease or infection caused by the plant pest an effective amount of at least one bacterial strain provided herein or active variant thereof.
  • the bacterial strain provided herein or active variant thereof may comprise at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, .
  • the bacterial strain provided herein or active variant thereof promotes a defensive response to the pathogen that causes the plant disease.
  • the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 12 to 10 16 CFU per hectare.
  • the effective compound may be a composition derived from at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the composition derived from a bacterial strain described herein is extracted from the culture broth.
  • a defensive response in the plant can be triggered after applying the bacterial strain provided herein or active variant thereof to the plant, but prior to pathogen challenge and/or after pathogen challenge of the plant treated with the bacterial strain provided herein or active variant thereof.
  • a defensive response in the plant can be triggered after applying the bacterial strain provided herein or active variant thereof to the plant, but prior to pest challenge and/or after pest challenge of the plant treated with the bacterial strain provided herein or active variant thereof.
  • the bacterial strain provided herein or active variant thereof induces resistance to one, two, three, four, five or more plant pathogens and/or pests described herein. In other methods, the bacterial strain provided herein or active variant thereof induces resistance to one, two, three, four, five or more fungal plant pathogens, insect pests or nematode pests described herein.
  • disease resistance is intended that the plants avoid the disease symptoms that result from plant-pathogen interactions. That is, pathogens are prevented from causing plant diseases and the associated disease symptoms, or alternatively, the disease symptoms caused by the pathogen are minimized or lessened as compared to a control.
  • pest resistance is intended that the plants avoid the symptoms that result from infection of a plant by a pest. That is, pests are prevented from causing plant diseases and the associated disease symptoms, or alternatively, the disease symptoms caused by the pest are minimized or lessened as compared to a control.
  • methods of improving plant health and/or improving an agronomic trait of interest comprising applying to a plant an effective amount of at least one bacterial strain provided herein or an active variant thereof or an active derivative thereof.
  • the bacterial strain provided herein or active variant thereof may comprise at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 12 to 10 16 CFU per hectare.
  • the effective compound may be a composition derived from at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the composition derived from a bacterial strain described herein is extracted from the culture broth.
  • Increased stress tolerance refers to an increase in the ability of a plant to decrease or prevent symptoms associated with one or more stresses.
  • the stress can be a biotic stress that occurs as a result of damage done to plants by other living organisms such as a pathogen (for example, bacteria, viruses, fungi, parasites), insects, nematodes, weeds, cultivated or native plants.
  • the stress can also be an abiotic stress such as extreme temperatures (high or low), high winds, drought, salinity, chemical toxicity, oxidative stress, flood, tornadoes, wildfires, radiation and exposure to heavy metals.
  • an effective amount of the bacterial strain or active variant thereof improves plant health or improves an agronomic trait of interest by a statistically significant amount, including for example, at least about 10% to at least about 20%, at least about 20% to about 50%, at least about 10% to about 60%, at least about 30% to about 70%, at least about 40% to about 80%, or at least about 50% to about 90% or greater.
  • the bacterial strain provided herein or active variant thereof, and/or a composition derived from a whole culture broth or supernatant thereof may have bioherbicide activity, by "bioherbicide” is intended that a bacterial strain provided herein or active variant thereof, and/or a composition derived from a whole culture broth or supernatant thereof, reduces the growth rate, development, or both the growth rate and development (as evidenced by reduced dry weight), possibly leading to death, of at least one target plant species.
  • the bioherbicide exhibits selective activity (host specificity) when exposed to one or more plants, so that a plant of interest is not affected by the bioherbicide, while one or more target plants, for example a weed species, is susceptible to the effects of the bioherbicide agent.
  • the bioherbicide comprises at least one bacterial strain deposited as AIP011864, AIP060073, AIP089963, AIP098363,
  • the bioherbicide comprises a supernatant, filtrate, or extract derived from at least one of bacterial strain AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494,
  • the a supernatant, filtrate, or extract described above is extracted from the whole culture broth or supernatant.
  • the bacterial strain provided herein or active variant thereof are applied in an effective amount.
  • An effective amount of a bacterial strain provided herein or active variant thereof is an amount sufficient to control, treat, prevent, inhibit the pathogen or pest that causes a plant disease, and/or reduce plant disease severity or reduce plant disease development.
  • the effective amount of the bacterial strain provided herein or active variant thereof is an amount sufficient to improve an agronomic trait of interest and/or to promote or increase plant health, growth or yield of a plant susceptible to a disease and/or infection by a plant pest.
  • the rate of application of the bacterial strain provided herein or active variant thereof may vary according to the pathogen or pest being targeted, the crop to be protected, the efficacy of the bacterial strain provided herein or active variant thereof, the severity of the disease, the climate conditions, the agronomic trait of interest to improve, and the like.
  • the rate of bacterial strain provided herein or active variant thereof is 10 7 to 10 16 colony forming units (CFU) per hectare.
  • the rate of bacterial strain provided herein or active variant thereof application is 3 x 10 7 to 1 x 10 n colony forming units (CFU) per hectare. (This corresponds to about 1 Kg to 10kg of formulated material per hectare).
  • the rate of bacterial strain provided herein or active variant thereof application is 3 x 10 7 to 1 x 10 16 colony forming units (CFU) per hectare; about lxlO 12 to about lxlO 13 colony forming units (CFU) per hectare, about lxlO 13 to about lxlO 14 colony forming units (CFU) per hectare, about lxlO 14 to about lxlO 15 colony forming units (CFU) per hectare, about lxlO 15 to about lxlO 16 colony forming units (CFU) per hectare, about lxlO 16 to about lxlO 17 colony forming units (CFU) per hectare; about lxlO 4 to about lxlO 14 colony forming units (CFU) per hectare; about lxlO 5 to about lxlO 13 colony forming units (CFU) per hectare; about lxlO
  • the rate of bacterial strain provided herein or active variant thereof application is at least about lxlO 4 , about lxlO 5 , about lxlO 6 , about lxlO 7 , about lxlO 8 , about lxlO 9 , about lxlO 10 , about lxlO 11 , about Ixl0 12 lxl0 13 , about lxlO 14 , lxlO 15 , about lxlO 16 , or about lxlO 17 colony forming units (CFU) per hectare.
  • CFU colony forming units
  • the rate of bacterial strain provided herein or active variant thereof application is at least lxlO 7 to at least about lxlO 12 CFU/hectare.
  • the bacterial strain provided herein or active variant thereof applied comprises the strain deposited as AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, or an active derivative of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, or an active derivative of any thereof.
  • the effective compound may be a composition derived from at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the composition derived from a bacterial strain described herein is extracted from the culture broth or supernatant.
  • Any appropriate agricultural application rate for a biocide can be applied in combination with the bacterial strain provided herein or active variant thereof disclosed herein and/or a composition derived therefrom.
  • Methods to assay for the effective amount of the bacterial strain provided herein or active variant thereof include, for example, any statistically significant increase in the control of the pathogen or pest targeted by the biocide. Methods to assay for such control are known. Moreover, a statistically significant increase in the control of plant health, yield and/or growth that occurs upon application of an effective amount of the bacterial strain provided herein or active variant thereof when compared to the plant health, yield and/or growth that occurs when no bacterial strain provided herein or active variant thereof is applied.
  • compositions comprising at least one bacterial strain provided herein or active variant thereof provided herein (i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant or any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant any of thereof) and/or a composition derived therefrom.
  • a composition derived therefrom i.e., AIP011864, AIP060073, AIP089963, AIP
  • applying is intended contacting an effective amount of the bacterial strain provided herein or active variant thereof to a plant, area of cultivation, seed and/or weed with one or more of the bacterial strain provided herein or active variant thereof so that a desired effect is achieved.
  • the application of the bacterial strain provided herein or active variant thereof and/or a composition derived therefrom can occur prior to the planting of the crop (for example, to the soil, the seed, or the plant).
  • the application of the bacterial strain provided herein or active variant thereof and/or a composition derived therefrom is a foliar application.
  • a further embodiment of the invention provides a method for controlling or inhibiting the growth of a plant pathogen by applying the population of bacterial strain provided herein or active variant thereof and/or a composition derived therefrom to an environment in which the plant pathogen and/or plant pest may grow.
  • the application may be to the plant, to parts of the plant, to the seeds of the plants to be protected, or to the soil in which the plant to be protected are growing or will grow.
  • Application to the plant or plant parts may be before or after harvest.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein and/or a composition derived therefrom is used as a foliar application to control or inhibit growth of one or more pathogens selected from the group consisting of Alternaria spp., Alternaria solani, Colletotrichum spp. , Mycosphaerella spp., Phomopsis spp., Cercospora spp., Botrytis spp., and Botrytis cinerea.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is used as a foliar application to control or inhibit growth of one or more insect or insect pests.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is applied to the soil in which the plants to be protected are growing or will grow to control or inhibit growth of one or more insect or insect pests.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is applied to the plant propagule (i.e. seed, slip, stem cutting, corn etc.) from which the plant to be protected are growing or will grow to control or inhibit growth of one or more insect or insect pests.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is applied to the plant tissue (including fruit) after harvest to control or inhibit growth of one or more insect or insect pests.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof provided herein is applied to the soil in which the plant to be protected are growing or will grow to control or inhibit growth of one or more pathogens selected from the group consisting of Rhizoctonia spp., Rhizoctonia solani, Fusarium spp., Sclerotium spp., Sclerotinia spp., Sclerotinia sclerotiorum, Phytopthora spp. , and Pythium spp.
  • an effective amount of at least one bacterial strain provided herein or active variant thereof and/or a composition derived therefrom is applied to the plant after harvest to control or inhibit growth of one or more pathogens selected from the group consisting of Monolinia spp., Penicillium spp., Botrytis ssp., and Botrytis cinerea.
  • the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips, anthers, and the like.
  • Grain is intended to mean the mature seed produced by commercial growers for purposes other than growing or reproducing the species.
  • the application of the bacterial strain provided herein or active variant thereof i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of
  • AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant thereof) and/or a composition derived therefrom is applied to the leaves of a soybean plant.
  • the timing of application can vary depending on the conditions and geographical location.
  • the bacterial strain provided herein or active variant thereof is applied at the Rl (beginning flowering stage) of soybean development or may be applied earlier depending on ASR onset and the disease severity.
  • the biocide to a crop, area of cultivation, or field it is intended that one or more of a particular field, plant crop, seed and/or weed is treated with one or more of the bacterial strain provided herein or active variant thereof and one or more biocide so that a desired effect is achieved.
  • Various methods are provided for controlling a plant pathogen and/or plant pest that causes a plant disease in an area of cultivation containing a plant susceptible to the plant disease.
  • the method comprises planting the area of cultivation with seeds or plants susceptible to the plant disease or pest; and applying to the plant susceptible to the disease or pest, the seed or the area of cultivation of the plant susceptible to the plant disease or pest an effective amount of at least one bacterial strain provided herein or active variant thereof (i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, or an active derivative or any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP
  • the method comprises applying to a plant susceptible to the disease or pest, a seed, or an area of cultivation of the plant susceptible to the disease or pest an effective amount of a composition comprising at least one bacterial strain provided herein or active variant thereof and/or a composition derived therefrom.
  • the bacterial strain provided herein or active variant thereof may comprise at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the effective amount of the bacterial strain provided herein or active variant thereof comprises at least about 10 12 to 10 16 colony forming units (CFU) per hectare.
  • the effective compound may be a composition derived from at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • the composition derived from a bacterial strain described herein is extracted from the culture broth.
  • the “yield” of the plant refers to the quality and/or quantity of biomass produced by the plant.
  • biomass is intended any measured plant product.
  • An increase in biomass production is any improvement in the yield of the measured plant product.
  • An increase in yield can comprise any statistically significant increase including, but not limited to, at least a 1% increase, at least a 3% increase, at least a 5% increase, at least a 10% increase, at least a 20% increase, at least a 30%, at least a 50%, at least a 70%, at least a 100% or a greater increase in yield compared to a plant not exposed to the bacterial strain provided herein or active variant thereof and/or a composition derived therefrom.
  • a method for increasing yield in a plant comprises applying to a crop or an area of cultivation an effective amount of a composition comprising at least one bacterial strain comprising AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, a spore or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, wherein said effective amount comprises at least about 10 12 to 10 16 colony forming units (CFU) per hectare, and wherein said composition controls
  • composition derived from a bacterial strain described herein is extracted from the culture broth.
  • an "area of cultivation” comprises any region in which one desires to grow a plant.
  • Such areas of cultivations include, but are not limited to, a field in which a plant is cultivated (such as a crop field, a sod field, a tree field, a managed forest, a field for culturing fruits and vegetables, etc.), a greenhouse, a growth chamber, etc.
  • a coated seed which comprises a seed and a coating on the seed, wherein the coating comprises at least one bacterial strain provided herein or active variant thereof and/or a composition derived therefrom.
  • the effective compound may be a composition derived from at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629,
  • composition derived from a bacterial strain described herein is extracted from the culture broth.
  • the bacterial strain provided herein or active variant thereof may comprise at least one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof; or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof.
  • said bacterial strain provided herein or active variant thereof is present on the seed at about 10 s CFU/seed to about 10 7 CFU/seed, at about 10 4 CFU/seed to about 10 8 CFU/seed, at about 10 4 CFU/seed to about 10 s CFU/seed, at about 10 s CFU/seed to about 10 6 CFU/seed, at about 10 6 CFU/seed to about 10 7 CFU/seed, or at about 10 7 CFU/seed to about 10 s CFU/seed.
  • the seed coating can be applied to any seed of interest (i.e., for a monocot or a dicot). Various plants of interest are disclosed elsewhere herein.
  • a seed coating can further comprise at least at least one nutrient, at least one herbicide or at least one pesticide, or at least one biocide. See, for example, US App Pub. 20040336049,
  • a plant of interest i.e., plant susceptible to the plant disease
  • the area of cultivation comprising the plant can be treated with a combination of an effective amount of the bacterial strain provided herein or active variant thereof and an effective amount of a biocide.
  • treated with a combination of or “applying a combination of a bacterial strain provided herein or active variant thereof and a biocide to a plant, area of cultivation or field it is intended that one or more of a particular field, plant, and/or weed is treated with an effective amount of one or more of the bacterial strain provided herein or active variant thereof and one or more biocide so that a desired effect is achieved.
  • the application of one or both of the bacterial strain provided herein or active variant thereof and the biocide can occur prior to the planting of the crop (for example, to the soil, or the plant).
  • the application of the bacterial strain provided herein or active variant thereof and the biocide may be simultaneous or the applications may be at different times (sequential), so long as the desired effect is achieved.
  • the active variant comprises a bacterial strain provided herein that is resistance to one or more biocide.
  • the bacterial strain provided herein or active variant thereof i.e., AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, or a spore, or a forespore or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof) is resistant to glyphosate.
  • a plant, crop, or area of cultivation is treated with a combination of an effective amount of the bacterial strain provided herein or active variant thereof that is resistant to glyphosate and an effective amount of glyphosate, wherein the effective amount of glyphosate is such as to selectively control weeds while the crop is not significantly damaged.
  • the active variant comprises a bacterial strain provided herein that is resistant to glufosinate.
  • a plant, crop, or area of cultivation is treated with a combination of an effective amount of the bacterial strain provided herein or active variant thereof that is resistant to glufosinate and an effective amount of glufosinate, wherein the effective amount of glufosinate is such as to selectively control weeds while the crop is not significantly damaged.
  • the effective amount of the bacterial strain provided herein or active variant thereof is sufficient to result in a statistically significant increase in plant health, yield, and/or growth when compared to the plant health, yield, and/or growth that occurs when the same concentration of a bacterial strain provided herein or active variant thereof that was not modified to be resistant to glufosinate is applied in combination with the effective amount of the glufosinate or active derivative thereof.
  • the bacterial strain provided herein or active variant thereof comprises an effective amount of AIP011864, AIP060073, AIP089963, AIP098363,
  • the bacterial strain provided herein or active variant thereof can be used in combination with a biocide (i.e., a herbicide, fungicide, pesticide, or other crop protection chemical).
  • a biocide i.e., a herbicide, fungicide, pesticide, or other crop protection chemical.
  • the bacterial strain provided herein or active variant thereof is compatible with the biocide of interest.
  • herbicide fungicide, pesticide, insecticide or other crop protection chemical tolerance or herbicide, fungicide, pesticide, insecticide or other crop protection chemical resistance
  • an organism i.e., the plant and/or the bacterial strain provided herein or active variant thereof etc.
  • Herbicides that can be used in the various methods and compositions discloses herein include glyphosate, ACCase inhibitors (Arloxyphenoxy propionate (FOPS)); ALS inhibitors (Sulfonylurea (SU)), Imidazonlinone (IMI), Pyrimidines (PM)); microtubule protein inhibitor (Dinitroaniline (DNA)); synthetic auxins (Phenoxy (P)), Benzoic Acid (BA), Carboxylic acid (CA)); Photosystem II inhibitor (Triazine (TZ)), Triazinone (TN), Nitriles (NT), Benzothiadiazinones (BZ), Ureas (US)); EPSP Synthase inhibitor (glycines (GC)); Glutamine Synthesis inhibitor (Phosphinic Acid (PA)); DOXP synthase inhibitor (Isoxazolidinone (IA)); HPPD inhibitor (Pyrazole (PA)), Triketone (TE)); PPO inhibitors
  • Pesticides that can be used in the various methods and compositions disclosed herein include imidacloprid clothianidin, arylpyrazole compounds (WO2007103076); organophosphates, phenyl pyrazole, pyrethoids caramoyloximes, pyrazoles, amidines, halogenated hydrocarbons, carbamates and derivatives thereof, terbufos, chloropyrifos, fipronil, chlorethoxyfos, telfuthrin, carbofuran, imidacloprid, tebupirimfos (U.S. Patent No. 5,849,320).
  • Fungicides that can be used in the various methods and compositions disclosed herein include aliphatic nitrogen fungicides (butylamine, cymoxanil, dodicin, dodine, guazatine, iminoctadine); amide fungicides (benzovindiflupyr, carpropamid, chloraniformethan, cyflufenamid, diclocymet, diclocymet, dimoxystrobin, fenaminstrobin, fenoxanil, flumetover, furametpyr, isofetamid, isopyrazam, mandestrobin, mandipropamid, metominostrobin, orysastrobin, penthiopyrad, prochloraz, quinazamid, silthiofam, triforine); acylamino acid fungicides (benalaxyl, benalaxyl-M, furalaxyl, metalaxyl, metalaxyl-M, pe
  • strobilurin fungicides fluoxastrobin, mandestrobin
  • methoxyacrylate strobilurin fungicides azoxystrobin, bifujunzhi, coumoxystrobin, enoxastrobin, flufenoxystrobin, jiaxiangjunzhi, picoxystrobin, pyraoxystrobin
  • methoxycarbanilate strobilurin fungicides pyraclostrobin, pyrametostrobin, triclopyricarb
  • methoxyiminoacetamide strobilurin fungicides dimoxystrobin, fenaminstrobin, metominostrobin, orysastrobin
  • methoxyiminoacetate strobilurin fungicides (kresoxim-methyl, trifloxystrobin); aromatic fungicides (biphenyl, chlorodinitronaphthalenes, chloroneb, chlorothalonil, cresol, dicloran, fenjuntong, hexachlorobenzene, pentachlorophenol, quintozene, sodium pentachlorophenoxide, tecnazene, trichlorotrinitrobenzenes); arsenical fungicides (asomate, urbacide); aryl phenyl ketone fungicides (metrafenone, pyriofenone); benzimidazole fungicides (albendazole, benomyl, carbendazim, chlorfenazole, cypendazole, debacarb, fuberidazole, mecarbinzid, rabenzazole, thiabendazole); benzimid
  • phenylmercuriurea phenylmercury acetate, phenylmercury chloride, phenylmercury derivative of pyrocatechol, phenylmercury nitrate, phenylmercury salicylate, thiomersal, tolylmercury acetate); morpholine fungicides (aldimorph, benzamorf, carbamorph, dimethomorph, dodemorph,
  • organophosphorus fungicides (ampropylfos, ditalimfos, EBP, edifenphos, fosetyl, hexylthiofos, inezin, iprobenfos, izopamfos, kejunlin, phosdiphen, pyrazophos, tolclofos-methyl, triamiphos); organotin fungicides (decaf entin, fentin, tributyltin oxide); oxathiin fungicides (carboxin, oxycarboxin); oxazole fungicides (chlozolinate, dichlozoline, drazoxolon, famoxadone, hymexazol, metazoxolon, myclozolin, oxadixyl, oxathiapiprolin, pyrisoxazole,
  • Non-limiting embodiments of the invention include: 1. A composition comprising:
  • spore, or a forespore, or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, wherein the active variant comprises a bacterial strain having a genome within a Mash distance of about 0.015, and wherein said spore, or forespore, or combination of cells, forespores and/or spores is present at about 10 s CFU/gram to about 10 12 CFU/gram or at about 10 s CFU/ml to about 10 12 CFU/ml; and/or
  • an effective amount of said composition improves an agronomic trait of interest of a plant or controls a plant pest or plant pathogen that causes a plant disease.
  • composition of embodiment 1 wherein the plant disease is a fungal plant disease.
  • composition of embodiment 1 or 2, wherein the plant disease is Asian Soybean Rust (ASR).
  • ASR Asian Soybean Rust
  • composition of any one of embodiments 1-5 wherein said composition comprises a wettable powder, a spray dried formulation, a liquid formulation, and/or a stable formulation.
  • composition of embodiment 7, wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina, Cercospora beticola, Alternaria solani, Rhizoctonia solani, Blumeria graminisf. sp. Tritici, Erysiphe necator, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe lagerstroemiae, Sphaerotheca pannosa, Colletotrichum cereale, Apiognomonia errabunda,
  • Plasmopara obduscens Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora capsici, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Gibberella zeae, Colletotrichum graminicola, Phakopsora sp., Phakopsora meibomiae, Phakopsora pachyrizi, Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia
  • composition of embodiment 8 wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina, Alternaria solani, Rhizoctonia solani, Erysiphe necator, Podosphaera xanthii, Colletotrichum cereal, Plasmopara viticola, Peronospora belbahrii, Pythium aphanidermatum, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusa
  • composition of embodiment 8, wherein said plant pathogen comprises
  • Phakopsora pachyrhizi or Phakopsora meibomiae Phakopsora pachyrhizi or Phakopsora meibomiae.
  • composition of embodiment 10, wherein said pathogen comprises Phakopsora pachyrhizi.
  • composition of embodiment 12, wherein the plant pest is selected from the orders Lepidoptera, Coleoptera, Trombidiformes, Thysenoptera, Acari, and Hemiptera.
  • composition of embodiment 12 or 13, wherein the plant pest is selected from the group consisting of a stink bug, aphid, whitefly, blackfly, thrips, mite, spider mite, fruit fly, corn rootworm, and Colorado potato beetle.
  • compositions of any of embodiments 12-14, wherein the plant pest is a southern green stink bug, western corn rootworm, green peach aphid, western flower thrips, or Colorado potato beetle.
  • a composition comprising a cell paste comprising:
  • an effective amount of said bacterial strain composition improves an agronomic trait of interest of the plant or controls a plant pest or plant pathogen that causes a plant disease.
  • composition of embodiment 15, wherein the plant disease is a fungal plant disease.
  • composition of any one of embodiments 16-17, wherein the plant disease is Asian Soybean Rust, Phytophthora late blight, or Rhizoctonia damping-off.
  • plant pathogen comprises at least one fungal pathogen.
  • composition of embodiment 19, wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina, Cercospora beticola, Alternaria solani, Rhizoctonia solani, Blumeria graminisf. sp. Tritici, Erysiphe necator, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe lager stroemiae, Sphaerotheca pannosa, Colletotrichum cereale, Apiognomonia errabunda,
  • Plasmopara obduscens Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora capsici, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Gibberella zeae, Colletotrichum graminicola, Phakopsora sp., Phakopsora meibomiae, Phakopsora pachyrizi, Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia
  • composition of embodiment 19, wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina, Alternaria solani, Rhizoctonia solani, Erysiphe necator, Podosphaera xanthii, Colletotrichum cereal, Plasmopara viticola, Peronospora belbahrii, Pythium aphanidermatum, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Phakopsora pachyrizi, Phakopsora meibomiae and Venturia inaequalisa.
  • fungal pathogens selected from the group consisting of Botrytis cinerea,
  • composition of embodiment 19, wherein said plant pathogen comprises
  • Phakopsora pachyrhizi or Phakopsora meibomiae Phakopsora pachyrhizi or Phakopsora meibomiae.
  • composition of embodiment 19, wherein said plant pathogen comprises
  • composition of embodiment 16, wherein said plant pest is a nematode pest and/or insect pest.
  • composition of embodiment 24, wherein the plant pest is selected from the orders Lepidoptera, Coleoptera, Trombidiformes, Thysenoptera, Acari, and Hemiptera.
  • the plant pest is a southern green stink bug, western corn rootworm, sweet potato weevil, green peach aphid, western flower thrips, or Colorado potato beetle.
  • composition comprising a wettable power comprising
  • an effective amount of said bacterial strain composition improves an agronomic trait of interest of the plant or controls a plant pest or plant pathogen that causes a plant disease.
  • composition of embodiment 28, wherein the plant disease is a fungal plant disease.
  • composition of embodiment 28 or 29, wherein the plant pathogen comprises at least one fungal pathogen.
  • composition of embodiment 30, wherein the said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina, Cercospora beticola, Alternaria solani, Rhizoctonia solani, Blumeria graminisf. sp. Tritici, Erysiphe necator, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe lager stroemiae, Sphaerotheca pannosa, Colletotrichum cereale, Apiognomonia errabunda,
  • composition of embodiment 31 wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina, Alternaria solani, Rhizoctonia solani, Erysiphe necator, Podosphaera xanthii, Colletotrichum cereal, Plasmopara viticola, Peronospora belbahrii, Pythium aphanidermatum, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Phakopsora pachyrizi, Phakopsora meibomiae, and Venturia inaequalisa.
  • Botrytis cinerea Cercospora sojina
  • composition of embodiment 31, wherein said plant pathogen comprises
  • Phakopsora pachyrhizi or Phakopsora meibomiae Phakopsora pachyrhizi or Phakopsora meibomiae.
  • composition of embodiment 33, wherein said plant pathogen comprises
  • composition of embodiment 28, wherein said plant pest is a nematode pest and/or insect pest.
  • composition of embodiment 35, wherein the plant pest is selected from the orders Lepidoptera, Coleoptera, Trombidiformes, Thysenoptera, Acari, and Hemiptera.
  • composition of any one of embodiments 35 or 36, wherein the plant pest is selected from the group consisting of a stink bug, aphid, whitefly, blackfly, thrips, mite, spider mite, fruit fly, corn rootworm, and Colorado potato beetle.
  • compositions of any one of embodiments 35-37, wherein the plant pest is a southern green stink bug, western corn rootworm, sweet potato weevil, green peach aphid, western flower thrips, or Colorado potato beetle.
  • composition of embodiment 39, wherein said active variant is selected under herbicide, fungicide, pesticide, insecticide, or other crop protection chemical pressure and is resistant to said herbicide, fungicide, pesticide, insecticide, or other crop protection chemical.
  • invention 49 The isolated biologically pure culture of embodiment 49, wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina, Cercospora beticola, Alternaria solani, Rhizoctonia solani, Blumeria graminis f. sp. Tritici, Erysiphe necator, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe lager stroemiae, Sphaerotheca pannosa, Colletotrichum cereale,
  • Apiognomonia errabunda Apiognomonia veneta, Colletotrichum gloeosporiodes, Discula fraxinea, Plasmopara viticola, Pseudoperonospora cubensis, Peronospora belbahrii, Bremia lactucae, Peronospora lamii, Plasmopara obduscens, Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora capsici, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Gibberella ze
  • invention 50 The isolated biologically pure culture of embodiment 50, wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina, Alternaria solani, Rhizoctonia solani, Erysiphe necator, Podosphaera xanthii, Colletotrichum cereal, Plasmopara viticola, Peronospora belbahrii, Pythium aphanidermatum, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Phakopsora pachyrizi, Phakopsora meibomiae, and Venturia inaequalisa.
  • fungal pathogens selected from the group consist
  • invention 58 wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina, Cercospora beticola, Alternaria solani, Rhizoctonia solani, Blumeria graminisf. sp. Tritici, Erysiphe necator, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe lager stroemiae, Sphaerotheca pannosa, Colletotrichum cereale, Apiognomonia errabunda,
  • Plasmopara obduscens Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora capsici, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Fusarium oxysporum, Fusarium graminicola, Gibberella zeae, Colletotrichum graminicola, Phakopsora sp., Phakopsora meibomiae, Phakopsora pachyrizi, Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia
  • invention 58 wherein said plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina, Alternaria solani, Rhizoctonia solani, Erysiphe necator, Podosphaera xanthii, Colletotrichum cereal, Plasmopara viticola, Peronospora belbahrii, Pythium aphanidermatum, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora nicotianae, Phytophthora infestans, Phytophthora tropicalis, Phytophthora sojae, Fusarium graminearum, Fusarium solani, Phakopsora pachyrizi, Phakopsora meibomiae, and Venturia inaequalisa.
  • Botrytis cinerea Cercospora sojina
  • a method for growing a plant susceptible to a plant pest or plant disease or improving an agronomic trait of interest in a plant comprising applying to the plant
  • CFU colony forming units
  • said effective amount controls a plant pest or plant pathogen that causes the plant disease or improves the agronomic trait of interest.
  • plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina, Cercospora beticola, Alternaria solani, Rhizoctonia solani, Blumeria graminisf. sp.
  • Tritici Erysiphe necator, Podosphaera xanthii, Golovinomyces cichoracearum, Erysiphe lagerstroemiae, Sphaerotheca pannosa, Colletotrichum cereale, Apiognomonia errabunda, Apiognomonia veneta, Colletotrichum gloeosporiodes, Discula fraxinea, Plasmopara viticola, Pseudoperonospora cubensis, Peronospora belbahrii, Bremia lactucae, Peronospora lamii, Plasmopara obduscens, Pythium cryptoirregulare, Pythium aphanidermatum, Pythium irregulare, Pythium sylvaticum, Pythium myriotylum, Pythium ultimum, Phytophthora capsici, Phytophthora nicotianae, Phy
  • plant pathogen comprises one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina,
  • a method of controlling a plant pest or plant pathogen that causes a plant disease in an area of cultivation comprising:
  • an effective amount of at least one bacterial strain comprising AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, wherein the active variant comprises a bacterial strain having a genome within a Mash distance of about 0.015, and wherein said effective amount comprises at least about 10 12 to 10 16 colony forming units (CFU) per hectare;
  • an effective amount of at least one bacterial strain comprising a spore, or a forespore, or a combination of cells, forespores and/or spores from any one of AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, AIP064474, AIP085152, AIP004618, AIP037827, AIP085784, or an active variant of any thereof, wherein the active variant comprises a bacterial strain having a genome within a Mash distance of about 0.015, and wherein said effective amount comprises at least about 10 12 to 10 16 colony forming units (CFU) per hectare; or (iii) an effective amount of a supernatant, filtrate, or extract derived from a whole cell culture of at least one of bacterial strain AIP011864, AIP060073, AIP089963, AIP098363, AIP054629, AIP038494, A
  • said effective amount controls a plant pest or plant pathogen that causes the plant disease or improves the agronomic trait of interest.
  • Phakopsora sp. Phakopsora meibomiae, Phakopsora pachyrizi, Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia spp., Venturia inaequalis, Verticillium spp, Erwinia amylovora, Monilinia fructicola, Monilinia lax, and Monilinia fructigena.
  • composition controls one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina,
  • a method of making a modified bacterial strain comprising:
  • a method of treating or preventing a plant disease comprising applying to a plant having a plant pest or plant disease or at risk of developing a plant pest or plant disease an effective amount of:
  • said effective amount of said bacterial strain, spore, or a forespore, or a combination of cells, forespores and/or spores or the active variant of any thereof is at least about 10 12 to 10 16 CFU per hectare, and wherein said bacterial strain controls the plant pest or plant pathogen that causes the plant disease.
  • invention 106 The method of embodiment 105, wherein the one or more fungal plant diseases comprise Asian Soybean Rust (ASR).
  • ASR Asian Soybean Rust
  • invention 109 The method of embodiment 108, wherein the one or more fungal pathogens are selected from the group consisting of Botrytis cinerea, Cersospora spp, Cercospora sojina,
  • Phakopsora sp. Phakopsora meibomiae, Phakopsora pachyrizi, Puccinia triticina, Puccinia recondita, Puccinia striiformis, Puccinia graminis, Puccinia spp., Venturia inaequalis, Verticillium spp, Erwinia amylovora, Monilinia fructicola, Monilinia lax, and Monilinia fructigena.
  • composition controls one or more fungal pathogens selected from the group consisting of Botrytis cinerea, Cercospora sojina,
  • Bacterial strains set forth in Table 2 were selected for assaying of pestieidal activity.
  • Bacterial strains set forth in Table 2 were cultured in CHA media which consists of, per L, NaCl (5g), tiyptone (lOg), nutrient broth (8g), CaCl 2 (0.14mM), MgCi 2 6H 2 0 (0.2mM), and MnCl 2 4H 2 0 (0.01 mM).
  • Table 3 summarizes the incubation time, the concentration of bacteria (CFU/ml) achieved and percentage of sporulation.
  • Tables 4 through 7 describe the constituents of the various growth media used.
  • Late blight caused by the oomycete pathogen Phytophthora infestans is a devastating disease of potato and tomato worldwide.
  • a leaf disc assay was performed using tomato cultivar 'Rutgers' . Plants of cultivar 'Rutgers' were planted every 2 weeks and placed inside a growth chamber (Percival
  • leaf discs (3.5-cm diameter each) were sprayed with 120 ⁇ of the bacterial strain at a concentration of 1 x 10 s spores/ml suspended in sterile distilled water.
  • Leaf disks were inoculated with 2 droplets (50 ⁇ per drop) of P. infestans mycelia and sporangia suspension a day after application of the bacterial strain, which was tested as a putative biocontrol againt.
  • the bacterial strain application was done using a fingertip sprayer (Container & Packaging Supply, Eagle, ID) fitted to a 15 mL conical centrifuge tube (Fisher Scientific, Cat No. l4-59-53A).
  • Leaf disks were placed with the abaxial side in contact with a saturated 20 x 20 cm filter paper (Whatman International Ltd., Kent, England) in a plastic container (Blister Box 20 x 20 cm, Placon, Madison, WI); two filter papers were used per box. Boxes with leaf disks were placed inside zip bags (Webster Industries, Peabody, MA) and incubated at 18°C in the dark for a period of 12 h. The boxes were then moved into a growth chamber (Percival Scientific, Inc) set at a cycle of 13 hours of light and l lh of darkness, maintained at 18°C and 95% RH. The experimental design was a randomized complete block design with 2 replications, and the experiment was repeated once.
  • ANOVA analysis of variance
  • Table 8 Pesticidal activity against P. infestans using leaf disk assay
  • Rhizoctonia solani infested grain was ground and then screened through a #10 screen to remove any over-sized grain.
  • the screened infested grain was added to Fafard Superfine Germination media at 1.5 grams of ground inoculum to 1 liter of soil mix by volume.
  • Germination mix, inoculum, and 1 liter of water per 75 liters of germination media was added to a cement mixer and mixed until everything was well incorporated.
  • the media-inoculum material was then placed into a secondary holding container which was sealed and held at 20°C for 18 hours before using in the assay.
  • 606-cell planting trays were filled with inoculated germination media.
  • One soybean seed was sown per 606 cell, planting at a depth of 1.5 to 2 cm leaving the planting holes open if applying treatments as a liquid formulation.
  • Individual planting cells were treated with one of the re-suspended cultures containing the strains set forth in Table 2 at 3 ml per cell/seed. The seed treatment was directly over the top of the seed. Once treatments were applied, the flats were shaken lightly to close planting holes. The planting trays were lightly watered and a humidity dome was placed on the flat. After 3-4 days flats were checked for moisture and lightly watered as needed to ensure cells were evenly moist. The humidity dome was replaced after watering.
  • Table 9 Pesticidal activity against 1.5g/L R. solani using soybean seed germination assay.
  • Example 4 Colorado Potato Beetle Leaf Disc Assay
  • a starter culture was prepared by filling a 96-well block with 1-ml (per well) LB media. Each well of the block was inoculated with a bacterial strain. This starter culture was grown at 30°C shaking at 225 rpms for 24 h. Assay cultures were prepared by filling two 48-well blocks with -1.7 ml (per well) media. Twenty-five ⁇ from each well of the starter culture was added to the assay culture blocks. Assay block were grown at 30 °C for either 24, 48 or 72 hrs at 225 rpms.
  • the assay blocks were removed from the incubator/shaker and centrifuged for 20 min at 4000 rpms in order to pellet the microbial content. The supernatant was then poured off so that only the pellet remained. Pellets were then re-suspended in 1.7 ml buffer and placed on ice until they were used in the assay. All microbial preparations were applied within 12 h of preparation.
  • a single prefilter was placed in each well of a 24-well plate. 50 ⁇ ddH20 was applied to each filter, to maintain the relative humidity throughout the experiment. Undamaged and uncurled potato leaves from a potato plant were selected for use. A #8 cork borer was used to make leaf discs, the center vein of the leaf was avoided. A single leaf disc was placed so the top-side of the leaf was facing up into each well of a 24-well plate, the leaf was placed so that it was not in contact with any of the sides of the plate. Microbial strains were provided as a liquid culture by microbiology in 48-well blocks with each well containing approx. 2250 ⁇ suspension. 100 ⁇ of 1% stock solution of surfactant (Silwet ECO spreader) was added to each well. The culture was thoroughly mixed and 40 ⁇ was pipetted onto a potato leaf disc. This process was repeated so that every bacterial treatment was applied to two leaf discs. The treatment was allowed to spread over the entire leaf.
  • surfactant Silwet ECO spreader
  • CPB eggs were purchased from French Agricultural Research, Inc., Lamberton, MN. After adding 5-6 larvae to each well, the plates were sealed with a pressure-sensitive adhesive cover and 4 small holes were added above each well. vPlates were then placed in a Percival incubator and maintained at 26 °C and 55% RH with 12/12 light:dark photoperiod for 24 h. After 24 h, plates were evaluated for the percent of each leaf disc that was consumed by the CPB larvae. Plates were than returned to the incubator.
  • Fall armyworm (FAW), corn earworm (CEW), European corn borer (ECB) southwestern corn borer (SWCB) and diamond backed moth (DBM) eggs were purchased from a commercial insectary (Benzon Research Inc., Carlisle, PA).
  • the FAW, CEW, ECB and BCW eggs were incubated to the point that eclosion occurred within 12 hrs of the assay setup.
  • SWCB and DBM were introduced to the assay as neonate larvae.
  • Assays were carried out in 24-well trays containing multispecies lepidopteran diet (SOUTHLAND PRODUCTS INCORPORATED, Lake Village, AR). Whole culture microbial suspensions were applied to the surface of the diet (diet overlay) and allowed to evaporate and soak into the diet.
  • the bioassay plates were sealed with a plate sealing film vented with pin holes. The plates were incubated at 26C at 65 RH on a 16:8 day:night cycle in a Percival for 5 days. The assays were assessed for level of mortality, growth inhibition and feeding inhibition. A microbe is considered active on CEW or FAW when mortality, growth inhibition and/or feeding inhibition is greater than the controls in three or more independent repetitions. The results are set forth in Table 10.
  • WCR Western corn rootworm
  • Southern Green stink bugs were reared at the AgBiome facility in RTP, NC. Only healthy second instar SGSB were used in the assay.
  • One ml of a 50:50 whole culture microbe: sucrose suspension was added to 4 wells of a 24 well plate, which was then covered with stretched parafilm that served as a feeding membrane to expose the SGSB to the diet/sample mixture.
  • the plates were incubated at 25 C:21C, 16:8 day:night cycle at 65%RH for 5 days. Mortality was assessed for each sample.
  • a microbe is considered active for SGSB when mortality is greater than 60% in three or more independent repetitions. The results are set forth in Table 10.
  • the following in vitro assay is used in the primary screen to determine if the bacterial culture or sample has contact activity against aphids (soybean aphids, Aphis glycines (SBA) and green peach aphids, Myzus persicae (GPA)).
  • This assay also is used to guide to determine the active portion or fraction of a bacterial culture, such as a whole cell broth, or of a supernatant derived from a whole cell broth, and to assist in determing its active components. Aphid colonies were initiated at AgBiome from insects from North Carolina State University and Michigan State University.
  • Leaf discs were removed from wells and submerged into treatment 3 times and then returned to each well. 24-well plates were placed under a fan to dry. Plates were re-sealed with a film held in Percival and observations for mortality, reproduction, and honeydew production were taken 3 days post-treatment. Bacterial cultures were considered active when high aphid mortality, high nymph mortality, low reproduction and low honey dew production were observed in three or more independent assays. The results are set forth in Table 10.
  • the following in vitro assay was performed to determine contact insecticidal activity of a bacterial strain against the aphid.
  • Bacterial strains grown in liquid media were removed from the incubator/shaker and centrifuged for 20 min at 4000 rpms in order to pellet the cells and cellular debris.
  • the supernatant (cell-free broth and/or spent media) was then separated from the pellet and diluted 0.5x.
  • the pellets were washed in buffer and then re-suspended in the growth volume.
  • the previously described method for aphid contact assay was used to determine the active fractions of AIP60073, AIP089963 and AIP085784 whole cell broths.
  • Results in Table 11 indicate the topically applied dilute supernatant is highly effective in controlling aphids, although activity was also retained in the pellet comprising the bacterial cells. This indicates that an effective compound, or metabolite, is present in the supernatant derived from cultures of the indicated bacterial strains. This effective compound is insecticidal.
  • the following describes a method for evaluating the pesticidal activity of the described bacterial strains against Asian Soybean Rust (ASR), using the fungal pathogen Phakopsora pachyrhizi.
  • ASR Asian Soybean Rust
  • the susceptible soybean cultivar Williams 82 is used in strain evaluation using the detached-leaf technique (Twizeyimana and Hartman, 2010) and using whole plant in growth chambers. Briefly, soybean seeds are sown in 18-cell plastic inserts that were filled with soil-less mix (Sunshine Mix, LCI ; Sun Gro Horticulture Inc., Bellevue, WA) and placed inside a flat. Ceils are fertilized at planting with slow-release pellets (Osmocote 19-6-12; 2 pellets per cm 2 ). Flats are maintained inside a growth chamber (Percival Scientific, Inc., Boone, IA) maintained at 70% relative humidity (RH) with a daily cycle of 14 h of light and 10 h of darkness at 24
  • Bacterial strains set forth in Table 2 are plated on Luria Bertani medium or in liquid culture, CHA medium which consists of, per L, NaCl (5g), tryptone (lOg), nutrient broth (8g), CaCl 2 (0.14mM), MgCl 2 6H 2 0 (0.2mM), and MnCl 2 4H 2 0 (0.01 mM) and are purified to obtain single colonies. Single colonies are characterized morphologically or using molecular techniques.
  • the Phakopsora pachyrhizi isolate FL07-1 is used in all ASR inoculations. The isolate is a single spore isolate obtained from infected soybean leaves collected from Gadseden County, Florida in 2007.
  • a detached leaf assay is performed to evaluate the pesticidal activity of the bacterial strains of Table 2. Briefly, leaf disks (3-cm diameter each) are sprayed with 120 ⁇ of the bacterial strain (1 x 10 s spores/ml of water) of P. pachyrhizi. Leaf disks are inoculated with 120 ⁇ spore suspension (1 x 10 4 spores/ml of sterile distilled water) of P. pachyrhizi a day after application of the bacterial strain. Both the bacterial strain and P. pachyrhizi inoculation applications are done using an atomizer attached to an air compressor (Twizeyimana and Hartman, 2010).
  • Leaf disks are placed adaxial side down on saturated 20 x 20 cm filter paper (Whatman International Ltd., Kent, England) in a plastic container (Blister Box 20 x 20 cm, Placon, Madison, WI); two filter papers are used per box. Boxes with leaf disks are incubated in the dark for a period of 12 h followed by a cycle of 13 hours of light (380 umol m-2s-l) and 11 h of darkness inside a tissue chamber (Percival Scientific, Inc.) maintained at 23°C and 95% RH. Prior to incubation, boxes are placed inside zip bags (Webster Industries, Peabody, MA). The experimental design is a randomized complete block design with 2 replications and is repeated once.
  • Bacterial strains are selected from the initial screening (conducted using leaf disk) based on their uredinia counts and are evaluated using whole plant assays. Soybean plants are maintained in growth chambers. When plants are at V2-stage (Fehr et al. 1971), the first fully expanded trifoliate leaf is sprayed with the bacterial strain, and the inoculation with P. pachyrhizi is done a day after as described in detached-leaf assay. Sprayed plants are maintained in a growth chamber at 75% RH with a daily cycle of 14 h and 10 h of light and darkness at 22°C and 24°C, respectively. The experimental design is a randomized complete block design with 3 replications and is repeated once.
  • Bacterial strains that have ⁇ 10 uredinia in the 1-cm diameter circle after evaluation in the whole plant assays described above, and/or active variants thereof, are applied to soybeans in the field. Treatments are applied at 16.8 Gallons/ Acre with treatments applied to achieve uniform plant coverage per general treatment guidelines for ASR treatment. The first treatment is applied at Rl with a follow up treatment applied at 14 days and 28 days after first treatment. The specific treatments are outlined below.
  • Example 11 Field Trials for the Various Bacterial Strains or Active Variants Thereof
  • Bacterial strain treatments are applied at 20 Gallons/ Acre with treatments applied to achieve uniform plant coverage per general treatment guidelines for ASR treatment.
  • the first treatment is applied at Rl with a follow up treatment applied at 14 days after first treatment.
  • the specific treatments are outlined below. Treatments:
  • Example 12 Methods of Formulation for the Various Bacterial Strains
  • Example 2 The following describes methods of formulation for the bacterial strains described in Table 2, and/or active variants thereof.
  • a wettable powder formulation was produced by adding 5% (by mass of pellet) of glycerol to the cell pellet and then mixed with a spatula. 20% (by mass) of Microcel E was transferred to a food processor and the glycerol/pellet is poured over the Microcel. This material was blended using the knife blade attachment of the food processor for not more than 10 seconds. The product was dried overnight at 40 ⁇ to a water activity of approximately 0.3. The dried powder formulation was stored in vacuum sealed mylar pouches at 22 ⁇ . The dried powder formulation retained antifungal activity and/or insecticidal activity. The concentration of each strain measured from the dried powder formulation is provided in Table 11.
  • the seed treatment formulation was made by mixing lOg formulated strain plus 30ml water plus 15ml Unicoat Polymer. The weighed out seed is placed in a sterilized mason jar. An appropriate amount of seed treatment solution based off of seed weight (.05ml/25g seed), the mixture is shaken for 60 seconds or until the seeds were visually well coated. The seeds are placed into a single layer in a foil roasting pan and placed under a laminar flow hood for 1 hour or until seeds are dry. Once the seeds dry, they are placed in an air tight container and stored at RT.
  • the experimental unit is a one-row plot of maize measuring approximately 12.5 feet. At 2 field sites, maize seeds treated with various bacterial strains recited in Table 2 are planted arranged using a Randomized Complete Block (RCB) design with four replications per treatment. Each experimental unit is infested artificially to augment naturally occurring populations. Up to 8 adjacent plants within each plot are manually infested with 500-750 corn rootworm eggs at an early vegetative stage (V2-V3). Efficacy against corn rootworm is estimated by sampling as many as five roots per plot.
  • RTB Randomized Complete Block
  • Bacterial strains are applied to the seeds. Bacterial strains may be applied to the seeds using a seed treatment formulation similar to that described in Example 14. A standard commercial seed-applied insecticide is also applied to the control seed. The rate of each insecticide is the labeled rate for control of western corn rootworm. Control substances are used to estimate insect injury potential (negative control).
  • Statistical analysis is performed. Means per treatment are calculated for each location and across locations. Maximum and minimum root scores within each event are used to compare treatments for the consistency of efficacy provided. Descriptive statistics are used to communicate trends in the data. Analyses consistent with a RCB design are used to determine statistical significance.
  • the experimental unit is a one-row plot of maize measuring approximately 12.5 feet. At 2 field sites, maize seeds are planted and bacterial strains recited in Table 2, or active variants thereof, are applied in a RCB design with two replications per treatment. Each experimental unit is infested artificially to augment naturally occurring populations. Timing of infestation and damage evaluation are dependent on lepidopteran species. Stalk tunneling, leaf feeding and/or ear feeding damage are evaluated.
  • the bacterial strains are applied to foliage.
  • a standard commercial foliar insecticide is applied at the label rate to the control plant foliage.
  • the negative control is used to estimate insect injury potential.
  • the number of thrips on two leaves per plant were counted on the 6th and 10th day after treatment (DAT). The mean for each day was calculated and data are summarized in Table 18.
  • Table 18 Insecticidal activity of bacterial strains against western flower thrips
  • the experimental unit is a mini-plot of horticultural crop such as strawberries with measurements/samples taken in a randomly placed 30 X 30 cm quadrant.
  • the plots are infested with insect(s) of interest, for example thrips, mites, aphids, and/or white flies, in addition to the naturally occurring populations.
  • Bacterial strains recited in Table 2, and/or active variants thereof, are applied via foliar spray in a RCB design with four replications per treatment. Appropriate parameters of insect infestation are sampled/measured such as for example "the number of thrips per 10 flowers".
  • the data are recorded at 1, 2, 6, 7 and 14 days post-treatment.
  • a standard commercial foliar insecticide is applied at the label rate, and the negative control is used to estimate insect injury potential.

Abstract

L'invention concerne des compositions et des procédés pour le traitement ou la prévention d'une maladie touchant les plantes et/ou destinés à des organismes nuisibles s'attaquant aux plantes. Ces compositions et ces procédés comprennent une souche bactérienne qui lutte contre un ou plusieurs organismes nuisibles et/ou pathogènes provoquant une maladie dans la plante, ou qui améliorent au moins un caractère agronomique d'intérêt dans une plante. La souche bactérienne peut être utilisée comme inoculant pour les plantes. Par conséquent, l'invention concerne des procédés de culture d'une plante sensible à une maladie s'attaquant aux plantes et/ou sensible à des organismes nuisibles s'attaquant aux plantes et des procédés pour lutter contre une maladie touchant les plantes et/ou contre des organismes nuisibles s'attaquant aux plantes sur une plante sensible à la maladie touchant les plantes et/ou sensible à des organismes nuisibles s'attaquant aux plantes.
PCT/US2018/043479 2017-07-26 2018-07-24 Compositions et procédés permettant d'améliorer la santé des plantes et de lutter contre les maladies touchant les plantes et les organismes nuisibles pour les plantes WO2019023226A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201762537269P 2017-07-26 2017-07-26
US62/537,269 2017-07-26
US201862621316P 2018-01-24 2018-01-24
US62/621,316 2018-01-24

Publications (1)

Publication Number Publication Date
WO2019023226A1 true WO2019023226A1 (fr) 2019-01-31

Family

ID=63207876

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/043479 WO2019023226A1 (fr) 2017-07-26 2018-07-24 Compositions et procédés permettant d'améliorer la santé des plantes et de lutter contre les maladies touchant les plantes et les organismes nuisibles pour les plantes

Country Status (1)

Country Link
WO (1) WO2019023226A1 (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109722396A (zh) * 2019-01-25 2019-05-07 黑龙江大学 同时去除低温地下水中腐殖酸和硝酸盐的假单胞菌及其应用
CN112544342A (zh) * 2020-12-10 2021-03-26 广东省林业科学研究院 一种高效中华鹅膏菌专用培养基及其制备方法与应用
CN113354491A (zh) * 2021-07-21 2021-09-07 广西壮族自治区农业科学院 一种生防与植物活力复合剂及其制备方法
CN114556032A (zh) * 2019-08-14 2022-05-27 博思特生物群落公司 用于预防或减少真菌病原体在植物上生长的微生物组合物
CN114544800A (zh) * 2022-01-14 2022-05-27 南通市疾病预防控制中心 分子筛串联固相萃取检测甲氧基丙烯酸酯类杀菌剂的方法
WO2022225926A1 (fr) 2021-04-19 2022-10-27 AgBiome, Inc. Compositions et procédés d'amélioration de la santé de plantes et de lutte contre les maladies de plantes
WO2022245786A1 (fr) 2021-05-18 2022-11-24 AgBiome, Inc. Compositions et méthodes pour améliorer la santé des plantes et lutter contre des maladies de plantes
CN114990009B (zh) * 2022-05-19 2023-07-21 东莞市农业科学研究中心 植物根际促生菌株f13在制备抗病促生增产微生物菌剂中的应用
EP4007490A4 (fr) * 2019-07-19 2023-12-27 Agriculture Victoria Services Pty Ltd Nouvelles souches de pseudomonas et procédés associés
WO2024026305A1 (fr) 2022-07-26 2024-02-01 AgBiome, Inc. Compositions et procédés basés sur pseudomonas chlororaphis pour améliorer la santé des plantes et lutter contre les maladies des plantes

Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4094097A (en) 1976-09-10 1978-06-13 Cornell Research Foundation, Inc. Method for preparing a pesticidally resistant rhizobium and agronomic composition thereof
US4306027A (en) 1976-09-10 1981-12-15 Cornell Research Foundation, Inc. Pesticidally resistant rhizobium and agronomic use thereof
WO1994010845A2 (fr) 1992-11-17 1994-05-26 Ciba-Geigy Ag Compositions microbicides synergiques
WO1996038576A1 (fr) 1995-06-01 1996-12-05 Signal Pharmaceuticals, Inc. Lignee de cellules parentes oligodendrogliales humaines
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5849320A (en) 1996-06-13 1998-12-15 Novartis Corporation Insecticidal seed coating
US6758044B2 (en) 2001-10-10 2004-07-06 Goodrich Pump & Engine Control Systems, Inc. Control system for positioning compressor inlet guide vanes
US7250561B1 (en) 1996-07-16 2007-07-31 Bayer Cropscience S.A. Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides
WO2007103076A1 (fr) 2006-03-01 2007-09-13 Bayer Cropscience, Lp Enrobage de semence a base de polymeres
US8124846B2 (en) 2000-10-30 2012-02-28 Bayer CropScience S.A.S. Herbicide-tolerant plants through bypassing metabolic pathway
US20140173979A1 (en) 2012-12-21 2014-06-26 Dow Agrosciences Llc Method of coating seed
US20150033811A1 (en) 2013-08-05 2015-02-05 Specialty Fertilizer Products, Llc Micronutrient-enhanced polymeric seed coatings
WO2016109396A1 (fr) * 2014-12-29 2016-07-07 Fmc Corporation Compositions de bacillus amyloliquefaciens rti472 et procédés d'utilisation pour favoriser la croissance de plante et traiter une maladie de plante
JP2016155862A (ja) * 2016-05-24 2016-09-01 住友化学株式会社 有害生物防除組成物および有害生物防除方法
WO2017040273A2 (fr) * 2015-08-28 2017-03-09 AgBiome, Inc. Compositions et procédés de lutte contre une maladie des plantes

Patent Citations (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4306027A (en) 1976-09-10 1981-12-15 Cornell Research Foundation, Inc. Pesticidally resistant rhizobium and agronomic use thereof
US4094097A (en) 1976-09-10 1978-06-13 Cornell Research Foundation, Inc. Method for preparing a pesticidally resistant rhizobium and agronomic composition thereof
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
WO1994010845A2 (fr) 1992-11-17 1994-05-26 Ciba-Geigy Ag Compositions microbicides synergiques
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
WO1996038576A1 (fr) 1995-06-01 1996-12-05 Signal Pharmaceuticals, Inc. Lignee de cellules parentes oligodendrogliales humaines
US5849320A (en) 1996-06-13 1998-12-15 Novartis Corporation Insecticidal seed coating
US7935869B2 (en) 1996-07-16 2011-05-03 Bayer S.A.S. Chimeric gene with several herbicide tolerance genes, plant cell and plant resistant to several herbicides
US7250561B1 (en) 1996-07-16 2007-07-31 Bayer Cropscience S.A. Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides
US8124846B2 (en) 2000-10-30 2012-02-28 Bayer CropScience S.A.S. Herbicide-tolerant plants through bypassing metabolic pathway
US6758044B2 (en) 2001-10-10 2004-07-06 Goodrich Pump & Engine Control Systems, Inc. Control system for positioning compressor inlet guide vanes
WO2007103076A1 (fr) 2006-03-01 2007-09-13 Bayer Cropscience, Lp Enrobage de semence a base de polymeres
US20140173979A1 (en) 2012-12-21 2014-06-26 Dow Agrosciences Llc Method of coating seed
US20150033811A1 (en) 2013-08-05 2015-02-05 Specialty Fertilizer Products, Llc Micronutrient-enhanced polymeric seed coatings
WO2016109396A1 (fr) * 2014-12-29 2016-07-07 Fmc Corporation Compositions de bacillus amyloliquefaciens rti472 et procédés d'utilisation pour favoriser la croissance de plante et traiter une maladie de plante
WO2017040273A2 (fr) * 2015-08-28 2017-03-09 AgBiome, Inc. Compositions et procédés de lutte contre une maladie des plantes
JP2016155862A (ja) * 2016-05-24 2016-09-01 住友化学株式会社 有害生物防除組成物および有害生物防除方法

Non-Patent Citations (26)

* Cited by examiner, † Cited by third party
Title
"Pesticide Specifications, Manual on development and use of FAO and WHO specifications for pesticides", 2004, CROP LIFE INTERNATIONAL, article "FAO Plant Production and Protection Papers - 173, prepared by the FAO/WHO Joint Meeting on Pesticide Specifications"
ANDREWS ET AL., BIOCHEM. J., vol. 252, 1988, pages 199 - 206
BAUR ET AL., PESTICIDE SCIENCE, vol. 51, 1997, pages 131 - 152
BISHOP ET AL., BMC BIOLOGY, vol. 7, no. 1, 2009, pages 1741 - 7007,7-3
CAMMUE ET AL., J. BIOL. CHEM., vol. 267, 1992, pages 2228 - 2233
CAMPBELL ET AL., PNAS USA, vol. 110, no. 14, 2013, pages 5540 - 45
CHEMICAL ABSTRACTS, Columbus, Ohio, US; abstract no. 66733-21-9
CHOI ET AL., J. MICROBIOL. METHODS, vol. 64, 2006, pages 391 - 397
CZAPLA; LANG, J. ECON. ENTOMOL., vol. 83, 1990, pages 2480 - 2485
CZAPLA; LANG, J. ECON. ENTOMOL., vol. 83, no. 6, 1990, pages 2480 - 2485
DUPONT ET AL., ISMEJ, vol. 6, 2012, pages 1625 - 1628
GODOY ET AL., FITOPATOL. BRAS., vol. 31, no. 1, 2006, pages 63 - 68
HANAHAN, D.: "Studies on transformation of Escherichia coli with plasmids", J. MOL. BIOL., vol. 166, 1983, pages 557 - 77
HU ET AL., PLANT MOL. BIOL., vol. 34, 1997, pages 949 - 959
KONSTANTINIDIS, K.T. ET AL., PNAS USA, vol. 102, no. 7, 2005, pages 2567 - 72
LIU ET AL., PLANT BIOLOGY, vol. 91, 1994, pages 1888 - 1892
MARRONE ET AL., J. OF ECONOMIC ENTOMOLOGY, vol. 78, 1985, pages 290 - 293
ONDOV ET AL., GENOME BIOLOGY, vol. 17, 2016, pages 132
PARKS ET AL., GENOME RESEARCH, vol. 25, 2015, pages 1043 - 1055
RICHTER, M. ET AL., PNAS, vol. 106, no. 45, 2009, pages 19126 - 31
SEIDMAN, C.E. ET AL.: "Current Protocols in Molecular Biology", 1994, JOHN WILEY AND SONS
SHACHI VARDHAN ET AL: "Diversity analysis of biocontrol Bacillus isolated from rhizospheric soil of rice-wheat (Oryza sativa-Triticum aestivum L.) at India", THE JOURNAL OF ANTIBIOTICS, vol. 66, no. 8, 22 May 2013 (2013-05-22), GB, pages 485 - 490, XP055308072, ISSN: 0021-8820, DOI: 10.1038/ja.2013.10 *
STACKEBRANDT E ET AL.: "Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology", INT J SYST EVOL MICROBIOL., vol. 52, no. 3, 2002, pages 1043 - 7
THOMMA ET AL., PLANT BIOLOGY, vol. 95, 1998, pages 15107 - 15111
VARGHESE, N.J. ET AL., NUCLEIC ACIDS RESEARCH, 6 July 2015 (2015-07-06)
WANG ET AL., J. CHEM. TECHNOL. BIOTECHNOL., vol. 85, 2010, pages 775 - 778

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109722396A (zh) * 2019-01-25 2019-05-07 黑龙江大学 同时去除低温地下水中腐殖酸和硝酸盐的假单胞菌及其应用
EP4007490A4 (fr) * 2019-07-19 2023-12-27 Agriculture Victoria Services Pty Ltd Nouvelles souches de pseudomonas et procédés associés
CN114556032A (zh) * 2019-08-14 2022-05-27 博思特生物群落公司 用于预防或减少真菌病原体在植物上生长的微生物组合物
CN112544342A (zh) * 2020-12-10 2021-03-26 广东省林业科学研究院 一种高效中华鹅膏菌专用培养基及其制备方法与应用
WO2022225926A1 (fr) 2021-04-19 2022-10-27 AgBiome, Inc. Compositions et procédés d'amélioration de la santé de plantes et de lutte contre les maladies de plantes
WO2022225925A1 (fr) 2021-04-19 2022-10-27 AgBiome, Inc. Compositions et méthodes pour améliorer la santé de plantes et lutter contre des maladies des plantes
WO2022245786A1 (fr) 2021-05-18 2022-11-24 AgBiome, Inc. Compositions et méthodes pour améliorer la santé des plantes et lutter contre des maladies de plantes
CN113354491A (zh) * 2021-07-21 2021-09-07 广西壮族自治区农业科学院 一种生防与植物活力复合剂及其制备方法
CN114544800A (zh) * 2022-01-14 2022-05-27 南通市疾病预防控制中心 分子筛串联固相萃取检测甲氧基丙烯酸酯类杀菌剂的方法
CN114990009B (zh) * 2022-05-19 2023-07-21 东莞市农业科学研究中心 植物根际促生菌株f13在制备抗病促生增产微生物菌剂中的应用
WO2024026305A1 (fr) 2022-07-26 2024-02-01 AgBiome, Inc. Compositions et procédés basés sur pseudomonas chlororaphis pour améliorer la santé des plantes et lutter contre les maladies des plantes

Similar Documents

Publication Publication Date Title
US20200221703A1 (en) Bacterial strains and their use for controlling plant disease
CN113631040B (zh) 控制植物害虫和改善植物健康的组合物和方法
WO2019023226A1 (fr) Compositions et procédés permettant d'améliorer la santé des plantes et de lutter contre les maladies touchant les plantes et les organismes nuisibles pour les plantes
CN113163771B (zh) 用于防治植物有害生物和改善植物健康的组合物和方法
US20200054022A1 (en) Compositions and methods for controlling plant pests and plant disease
WO2022245786A1 (fr) Compositions et méthodes pour améliorer la santé des plantes et lutter contre des maladies de plantes
WO2020006555A1 (fr) Compositions comprenant des bactéries et procédés de lutte contre des organismes nuisibles de plantes et d'amélioration de la santé des plantes
WO2019074813A1 (fr) Compositions et procédés permettant d'améliorer la santé des plantes et de lutter contre les maladies touchant les plantes et les organismes nuisibles pour les plantes
US20220312774A1 (en) Compositions and methods for improving plant health and controlling plant disease
WO2022225925A1 (fr) Compositions et méthodes pour améliorer la santé de plantes et lutter contre des maladies des plantes
WO2019241370A1 (fr) Compositions et procédés d'amélioration de la santé des plantes et de lutte contre les maladies des plantes
WO2024026305A1 (fr) Compositions et procédés basés sur pseudomonas chlororaphis pour améliorer la santé des plantes et lutter contre les maladies des plantes
BR112018004081B1 (pt) Composições que compreendem uma cepa bacteriana, método para cultivar uma planta suscetível a uma doença, métodos para controlar um ou mais patógenos de plantas, método para o tratamento ou prevenção de uma doença de planta e uso das ditas composições para controlar doenças de plantas

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 18755355

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 18755355

Country of ref document: EP

Kind code of ref document: A1