WO2019004585A1 - Système de supplémentation et procédé de supplémentation d'agcl pour capteur de dispositif de surveillance continue du glucose - Google Patents

Système de supplémentation et procédé de supplémentation d'agcl pour capteur de dispositif de surveillance continue du glucose Download PDF

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WO2019004585A1
WO2019004585A1 PCT/KR2018/005128 KR2018005128W WO2019004585A1 WO 2019004585 A1 WO2019004585 A1 WO 2019004585A1 KR 2018005128 W KR2018005128 W KR 2018005128W WO 2019004585 A1 WO2019004585 A1 WO 2019004585A1
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voltage
agcl
glucose
working electrode
constant
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PCT/KR2018/005128
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English (en)
Korean (ko)
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차근식
남학현
이석원
강영재
정인석
심정수
나지선
이진선
곽수민
양현희
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주식회사 아이센스
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Publication of WO2019004585A1 publication Critical patent/WO2019004585A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
    • A61B5/7225—Details of analog processing, e.g. isolation amplifier, gain or sensitivity adjustment, filtering, baseline or drift compensation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry

Definitions

  • the present invention relates to an AgCl replenishment system and a replenishment method for a CGMS sensor, and more particularly, to a method and system for replenishing an AgCl replenishment system for a CGMS sensor, and more particularly, to a method and apparatus for analyzing a blood sample concentration by chronoamperometry.
  • a method and system for replenishing an AgCl replenishment system for a CGMS sensor and more particularly, to a method and apparatus for analyzing a blood sample concentration by chronoamperometry.
  • it is possible to minimize a deviation caused by the interfering substance by applying a stair-stepped ladder-shaped perturbation voltage for a short time, and also to provide a reference electrode with a longer life And an AgCl replenishment system and a replenishment method for the sensor.
  • the sensors of the continuous blood glucose measurement system currently being manufactured in the major markets of the United States and Europe are inserted into the subcutaneous tissue layer under the skin of the human skin to quantitatively measure the glucose of the interstitial fluid, Has been shown to correlate with blood glucose levels, so we make real-time measurements of the body's blood glucose by applying corrections between the two.
  • This allows the diabetic patient to avoid the inconvenience of the finger-prick method, which had to manage his blood sugar by piercing his fingertips with a needle, to continue blood glucose measurement for 1 to 2 weeks through insertion of one sensor body
  • blood glucose level change can be observed in real time, it is possible to promptly respond to hyperglycemia and hypoglycemic state of a patient, and systematic blood glucose control can be achieved.
  • glucose is oxidized via glucose oxidase-catalyzed reaction, which produces gluconolactone and hydrogen peroxide by oxygen in body fluid, and hydrogen peroxide is electronically oxidized, It is associated with glucose concentration.
  • biosensors are intended to be implanted within or into the body of living animals such as mammals.
  • these biosensors have a three-electrode system with a working electrode sensitive to the object of interest, a reference electrode for controlling the potential of the working electrode, and a counter electrode through which the electrical current generated on the working electrode passes .
  • reference and counter electrodes may be combined into one electrode to form a two-electrode system.
  • the working electrode may consist of a sensing layer, usually in direct contact with the conductive material of the electrode, and a diffusion-limited membrane layer on top of the sensing layer.
  • the reference electrode is made of Ag / AgCl, which is usually prepared by screen printing or electroplating.
  • the lifetime of the screen-printed Ag / AgCl reference electrode is limited because it dissolves into the surrounding tissue of AgCl in the body current sensor.
  • the lifetime of the entire sensor is often limited by the amount of Ag / AgCl available as a reference electrode of the sensor, particularly for a biosensor implanted in the body, increasing the level of Ag / AgCl placed on the reference electrode It is very important to keep the biosensor compact and compact.
  • the electrical signal output may be influenced by the diffusion coefficient toward the electrode of the blood sugar due to the change of the blood properties such as viscosity or the change of the reaction rate on the electrode surface,
  • a method of estimating and reflecting the degree of interference of a measurement object by giving various types of input signals to the sensor in order to minimize or eliminate the influence of interference caused by various variables such as external environment and property of the sample,
  • the parameter values derived from the determined signal are mathematically determined, such as by multiple linear regression
  • the interference level is estimated and the measured value is determined.
  • the present invention has been made in order to solve such problems, and it is an object of the present invention to provide a method and apparatus for measuring a blood sample concentration by chronoamperometry, It is possible to minimize the variation caused by the interfering material by applying a stair-stepped staggered waveform voltage for a short period of time, as well as to provide a reference electrode with a longer life, A supplementary system and a supplementary method.
  • An object of the present invention is to provide a AgCl replica for a CGMS sensor having an Ag / AgCl reference electrode that can extend the lifespan suitable for a current measurement sensor that can be used for a long period of time, System and a supplementary method.
  • the AgCl replenishment system for a CGMS sensor includes a sensor unit capable of being transplanted into a body and formed in multiple layers so as to surround an outer circumferential surface of the tube and measuring the concentration of glucose in the body, A constant DC voltage for initiating an oxidation-reduction reaction of glucose and for promoting an electron transfer reaction, and a step for applying a stair-stepped ladder-type perturbation voltage in order to complement AgCl of the reference electrode of the sensor unit following the constant DC voltage And a microcontroller for controlling the digital-to-analog converter circuit and determining whether the concentration value of the glucose is within an error range by using the ⁇ -shaped stepped ladder-type perturbation voltage have.
  • the AgCl replenishing method for a CGMS sensor is characterized in that the sensing device is implanted into the body to initiate the redox reaction of the analyte with respect to the working electrode of the sensor part for measuring the concentration of the analyte in the body, And a ladder-shaped perturbation voltage in the form of a ⁇ -shape is applied to the constant DC voltage to supplement AgCl of the reference electrode of the sensor unit.
  • the AgCl replenishment system and the replenishment method for the CGMS sensor according to an embodiment of the present invention can be used to measure the blood sample concentration by chronoamperometry and to measure the concentration of various interfering substances in the blood, When the deviation is large, a staggered ladder-shaped perturbation voltage is applied for a short time to minimize a variation caused by the disturbing material, and the reference electrode having an extended life can be provided and used for a long period of time.
  • the AgCl replenishment system and the replenishment method for the CGMS sensor according to an embodiment of the present invention can improve the accuracy of measurement, and can be miniaturized or compacted, and can be used for a long time. Can be provided.
  • FIG. 1 is a schematic diagram of a continuous blood glucose measurement system to which an AgCl replenishing system for a CGMS sensor according to an embodiment of the present invention is applied.
  • FIG. 2 is a schematic view of a sensing section used in a continuous blood glucose measurement system to which an AgCl replenishing system for a CGMS sensor according to an embodiment of the present invention is applied.
  • FIG. 3 is a configuration diagram of a sensing device used in a continuous blood glucose measurement system to which an AgCl replenishing system for a CGMS sensor according to an embodiment of the present invention is applied.
  • FIG. 4 is a graph showing a ⁇ -stepladder-type perturbation potential used in an AgCl replenishing method for a CGMS sensor according to an embodiment of the present invention and a corresponding induced current.
  • FIG. 5 is a flowchart illustrating an AgCl replenishing method for a CGMS sensor according to an embodiment of the present invention.
  • FIGS. 6 and 7 are graphs respectively showing the current value measured at the working electrode of the CGMS sensor, the applied voltage applied thereto, and the amount of AgCl according to the time of the reference electrode, respectively.
  • FIGS. 8 to 10 are graphs showing the relationship between the voltage applied for the concentration measurement of the analyte in the CGMS sensor according to an embodiment of the present invention, the current value measured at the working electrode of the CGMS sensor,
  • FIG. 3 is a graph showing the amount of AgCl in the electrode according to time.
  • FIG. 11 to 13 are graphs showing voltages applied to the in-vivo analyte concentration of a CGMS sensor according to an embodiment of the present invention, current values measured at the working electrode of the CGMS sensor,
  • FIG. 3 is a graph showing the amount of AgCl in the electrode according to time.
  • FIG. 1 is a schematic view of a continuous blood glucose measurement system to which an AgCl replenishment system for a CGMS sensor according to an embodiment of the present invention is applied.
  • FIG. 2 is a schematic diagram of a continuous blood glucose measurement system using an AgCl replenishment system for a CGMS sensor according to an embodiment of the present invention.
  • Fig. 3 is a schematic view of a sensing section used in Fig.
  • a continuous blood glucose measurement system 100 includes an insulin tank 110, an insulin pump 130, . ≪ / RTI >
  • Insulin is stored in the insulin tank 110.
  • the insulin pump 130 provides power for supplying insulin.
  • the sensing device 150 may measure the concentration of blood glucose and beta-hydroxybutyrate and directly inject insulin into the human body.
  • the insulin tank 110 and the insulin pump 130 may be connected to the microtubule 112 or the like and the insulin stored in the insulin tank 110 may be connected to the insulin pump 130 through the micro- Lt; / RTI >
  • the insulin delivered to the insulin pump 130 may be administered to the human body through the internal passage of the sensing unit 160.
  • the sensing device 150 includes an insulin tube 152 connected to the insulin pump 130 to directly scan the insulin in the body, and a plurality of layers formed to surround the outer circumferential surface of the insulin tube 152, And a potential applying unit 161 for applying a potential between the reference electrode and the working electrode of the sensor unit 160 for measuring the concentration of beta-hydroxybutyrate.
  • the insulin tube 152 may be formed in various forms that can be inserted into a body such as a cannula or a microdialysis tube.
  • the insulin tube 152 may be an insulative material, .
  • the sensor unit 160 is inserted into the body and includes a first working electrode 162, a second working electrode 164 and a reference electrode 166, a first reaction unit 168 and a second reaction unit 170 ).
  • the first working electrode 162 is formed to surround the outer circumferential surface of the insulin tube 152 and the first reaction unit 168 is formed on one side of the outer circumferential surface of the first working electrode 162. In the first reaction unit 168, redox reaction of glucose in the body occurs.
  • the second working electrode 164 is formed to surround the outer circumferential surface of the first working electrode 162 and the second reaction unit 170 is formed on one side of the outer circumferential surface of the second working electrode 164.
  • the second reaction unit 170 an oxidation-reduction reaction of beta-hydroxybutyrate occurs in the body.
  • a first insulating portion 172 may be formed between the first and second working electrodes 162 and 164 and the first and second working electrodes 162 and 164 may be electrically .
  • the first and second reaction units 168 and 170 may be conductive films including enzymes that cause oxidation of glucose and beta-hydroxybutyrate, respectively.
  • the material of the first and second working electrodes 162 and 164 through which the signal current is generated may be platinum, gold, carbon, or the like as a conductive material.
  • the reference electrode 166 is formed to surround the outer circumferential surface of the second working electrode 164.
  • a second insulating portion 174 may be formed between the second working electrode 164 and the reference electrode 166 and the second working electrode 164 and the reference electrode 166 ) Are electrically separated.
  • the reference electrode 166 may be substantially comprised of silver / silver chloride (Ag / AgCl).
  • glucose and beta-glucose levels are measured through current signals due to glucose and beta-hydroxybutyrate oxidation in the first and second reaction units 168 and 170, - While measuring the concentration of hydroxybutyrate, insulin can be administered into the body through the insulin tube 152. Therefore, the user can avoid the inconvenience of attaching a measuring instrument for measuring blood sugar and the like, and an insulin pump for insulin administration to the body surface separately.
  • the first and second reaction units 168 and 170 may be formed adjacent to the first and second reaction parts 168 and 170 so as not to be directly exposed to the body insertion direction of the reaction part 160.
  • the first and second reaction portion protecting portions 176 and 178 may be formed of the same material as that of the first and second insulating portions 172 and 174.
  • the potential applying unit 161 applies a potential to each of the working electrodes WE 162, 164 (see FIG. 1) to the reference electrode 166 so that the oxidation reaction of glucose and beta-hydroxybutyrate occurs in the first and second reaction units. ) Through the external electric power.
  • the first and second reaction units 168 and 170 are described for oxidizing glucose and beta-hydroxybutyrate, respectively.
  • the first and second reaction units 168 and 170 may be configured to oxidize beta-hydroxybutyrate and glucose, respectively.
  • FIG. 3 is a configuration diagram of a sensing device used in a continuous blood glucose measurement system to which an AgCl replenishment system for a CGMS sensor according to an embodiment of the present invention is applied.
  • FIG. The ⁇ -stepladder-type perturbation potential and the corresponding current obtained in correspondence with the ⁇ -stepped ladder-type perturbation potential.
  • the sensing device 150 used in the continuous blood glucose measurement system to which the AgCl replenishment system for a CGMS sensor according to an embodiment of the present invention is applied includes a sensing unit, preferably an electrochemical biosensor 160 Is connected to the connector 151, the connector 151 is electrically connected to the current-to-voltage converter 152 so that the microcontroller 155 (MCU) applies a constant voltage according to the existing large-time current method And a perturbation voltage can be applied to the potential applying unit 161 of the sensing unit 160 through a digital-analog converter circuit (DAC) 153 provided in the sensing device 100 without a separate perturbation voltage circuit .
  • DAC digital-analog converter circuit
  • the firmware of the sensing device 150 used in the continuous blood glucose measurement system to which the AgCl replenishing system for a CGMS sensor according to an embodiment of the present invention is applied first stores a constant capable of generating a predetermined perturbation voltage in a memory, A predetermined constant is written in a register of the DAC 153. When a perturbation voltage is applied, a constant value stored in the memory is incremented / decremented by a predetermined period of time and written into a register of the DAC 153.
  • the microcontroller 155 applies a corresponding voltage between the two electrodes of the sensing unit 160 according to a constant value recorded in the DAC 153 register.
  • the first or second sensing current measured through the sensing unit 160 is measured through the connector 151 and the current-to-voltage converter 152 directly through the analog-to-digital converter circuit 154 (ADC) .
  • ADC analog-to-digital converter circuit 154
  • the construction of the perturbation voltage by the stepped wave as shown in FIG. 4 is advantageous in that the circuit is simpler than the methods using the AC or linear scanning method, The measurement accuracy can be improved.
  • This fluctuation or perturbation causes a significant change in the characteristics of the induced current, which can be obtained by taking the current value obtained from one step or a plurality of steps constituting the stepped ladder type, It can be a means.
  • the reason why the response current is represented by the first response current or the second response current is that the characteristics of the response current are changed due to fluctuations or fluctuations and are different from each other.
  • a stepped ladder-type perturbation voltage application scheme with a periodicity that is additionally applied for a short period of time in order to remove the influence of the hematocrit ratio in the calibration equation after a constant voltage is called " ⁇ -stepladder perturbation potential Quot;) or simply a " stepladder potential ".
  • the currents with different characteristics above are the currents that can be used as variables to effectively separate or correct the effect of the hematocrit by the difference between the blood sugar and the hematocrit (hematocrit) dependence.
  • the current values of the first and second response currents are determined according to the blood sugar and the hematocrit (G 1 , g 2 ) of blood glucose and hematocrit ratio as follows.
  • the response current obtained by applying the stepped ladder-type perturbation voltage can not be obtained because the degree of fluctuation of the sample near the electric double layer continuously changes when each step is raised or lowered for a short time, The influence of the charging current also changes, and the characteristics of the current can be greatly different from the current obtained by the large-time current method.
  • the calibration method used in the method of measuring the concentration of the analyte in the biological sample according to an embodiment of the present invention
  • the feature points are called characteristic points and the current values of the feature points are used as they are or are appropriately modified to be variables suitable for use in the calibration equation.
  • the response current of the large time current method can be approximated by the cotrel equation when the reagent of the biosensor reaches a uniform liquid state with the sample in the sample cell.
  • F is the Faraday constant
  • A is the electrode area
  • D is the number of electrons in the sample of the oxidized / reduced material (e.g.,
  • And C is the concentration of the substance to be oxidized / reduced.
  • the characteristic point in the large time current method section is a current value at a point where the constant voltage is applied and is stably expressed in a cortical manner.
  • a time period of several seconds to several minutes It is within 3 ⁇ 10 seconds.
  • the second induction current obtained from the stepped ladder-type perturbation voltage is greatly different in characteristics from the first induction current obtained when a constant voltage is applied, and can be used as a variable having a high orthogonality in the entire verification equation .
  • a method of finding a feature point from second response currents corresponding to a period in which the perturbation voltage is applied and a method of creating a feature using the feature points are as follows.
  • the following method is one example, and it can be applied variously according to the purpose of application.
  • the characteristic points are searched for in the second response currents corresponding to the period in which the perturbation voltage is applied, or the current values obtained from the characteristic points are made into features, and they are combined in a linear manner to perform multivariable regression analysis ), It is possible to obtain a calibration equation that minimizes the effect of the hematocrit ratio.
  • FIG. 5 is a flow chart for explaining the AgCl replenishing method for a CGMS sensor according to an embodiment of the present invention
  • FIGS. 6 and 7 are graphs each showing a current value generally measured at the working electrode of the CGMS sensor
  • FIGS. 8 to 10 are graphs showing a voltage applied for measuring the concentration of a substance to be analyzed in the CGMS sensor according to an embodiment of the present invention
  • a voltage applied to the CGMS sensor 11 to 13 are graphs showing current values measured at the working electrode, the applied voltage applied thereto, and the amount of AgCl according to the time of the reference electrode.
  • a redox reaction of glucose is initiated in a sensing unit 160 implanted in the body,
  • the constant DC voltage for measuring the glucose value is applied (S140). Then, in order to obtain the AgCL regeneration and the new third sensing current, Shaped staggered ladder-type perturbation voltage is applied (S150) after the application of the second ⁇ -shaped stepped ladder-type perturbation voltage.
  • the glucose measurement is continued by applying the constant DC voltage and the stepped ladder-type perturbation voltage of the first ⁇ shape at step S160.
  • a first direct current voltage is applied to the working electrode to obtain a first induction current at least one time point
  • a first ⁇ -shaped stepped ladder-type perturbation voltage is applied after the constant direct current voltage is applied, (S110)
  • calculating a predetermined feature from the first or second response currents and determining at least one or more of the at least two or more interfering substances in the biological sample
  • the AgCl of the reference electrode is dissolved by using a calibration equation composed of a feature function to determine a predetermined new feature when the dissolved value of the measured glucose value is out of the error range, Shaped staggered ladder-type perturbation voltage having a frequency increased to facilitate the supplementation of the second ⁇ -shaped system It is configured to apply a trapezoidal-shaped localized perturbation voltage.
  • a concentration of AgCl on the reference electrode of the electrochemical sensing unit 160 is set between a reference electrode and another electrode by a perturbation voltage Lt; / RTI >
  • the reference electrode if at least a portion of the reference electrode is subcutaneously subcutaneously implanted into the patient, a portion of the AgCl on the reference electrode will dissolve into the aqueous environment. As a result of the AgCl loss, the electrochemical sensor can not maintain a reasonable potential over the measurement period, but for this exact measurement, the frequency applied to the reference electrode is increased by an ⁇ -shaped stepped ladder- Ag will result in Ag + . At this time, Ag + binds to Cl - in the body environment to form a silver salt (AgCl) on the reference electrode to supplement the concentration of AgCl in the reference electrode.
  • AgCl silver salt
  • the electrical potential applied to the reference electrode should be at a sufficient level for a time sufficient for Ag to be converted to a sufficient level of Ag + .
  • the perturbation voltage to be applied may be at least about +50 mV, +75 mV, +100 mV, +125 mV, +150 mV, +175 mV, +200 mV, +250 mV, In addition, the perturbation voltage may be applied for a period of at least 30 seconds, 45 seconds, 1 minute, 2 minutes, 3 minutes, or more, or for a measurement time of glucose.
  • the period of application of the perturbation voltage can be controlled to a time sufficient for Ag conversion.
  • a lower level of perturbation voltage such as +50 mV
  • the perturbation voltage applied may be reduced.
  • the application of the perturbation voltage may also be repeated one or more times while the reference electrode is positioned under the patient. This period may last from about one to three days, five days, one week, two weeks, about three weeks, one month, two months or more. Likewise, the application of the perturbation voltage may be repeated twice, three times, four times, five times, six times, seven times, eight times, nine times, and ten more times. It will be apparent to those skilled in the art that the number of times of application of the perturbation voltage is limited by the concentration of Ag of the reference electrode that can be used for the AgCl transformation to compensate for the concentration of AgCl on the reference electrode.
  • the sensing unit 160 can perform the system calibration or the user calibration through the application of the perturbation voltage.
  • This calibration may be done automatically without user involvement.
  • the calibration may be performed in a system based on real-time, depending on various factors such as, for example, glucose concentration and / or temperature under or glucose change rate, ≪ / RTI >
  • FIGS. 6 to 13 reference will be made to a reference electrode supplemented with an AgCl replenishment method for a CGMS sensor according to an embodiment of the present invention.
  • the standard reduction potential of glucose is 0.4 V
  • 0.4 V is applied to the constant DC voltage
  • the area of the reference electrode is 0.0314 cm 2
  • the area of the working electrode is 0.0031 cm 2
  • the initial value of AgCl on the reference electrode is 0.0450 mg
  • the current value measured at the working electrode is 8 hours
  • the first current is not measured after 8 hours, and the amount of AgCl is gradually decreased, which is almost zero at the time of 8 hours.
  • the application of the ⁇ -shaped trapezoidal perturbation voltage The life of the reference electrode is prolonged.
  • the AgCl replenishment system and the replenishment method for the CGMS sensor according to an embodiment of the present invention can be used to measure the blood sample concentration by chronoamperometry and to measure the concentration of various interfering substances in the blood, When the deviation is large, a staggered ladder-shaped perturbation voltage is applied for a short time to minimize a variation caused by the disturbing material, and the reference electrode having an extended life can be provided and used for a long period of time.

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Abstract

Un mode de réalisation de la présente invention concerne un système de supplémentation d'AgCl pour un capteur de dispositif de surveillance continue du glucose, lequel système peut comprendre : une unité de capteur apte à être implantée dans un corps et formée de multiples couches de façon à entourer la surface périphérique externe d'un tube pour mesurer la concentration de glucose dans le corps ; un circuit de convertisseur numérique-analogique pour appliquer une tension continue constante de façon à déclencher une réaction d'oxydo-réduction du glucose par rapport à l'unité de capteur et de façon à favoriser une réaction de transfert d'électrons, et une tension de perturbation du type en échelle échelonnée d'une forme lambda "Λ" pour supplémenter l'AgCl dans une électrode de référence de l'unité de capteur suivant la tension continue constante ; et un micro-dispositif de commande pour commander le circuit de convertisseur numérique-analogique et pour déterminer si une valeur de concentration de glucose se trouve ou non à l'intérieur d'une plage d'erreurs à l'aide de la tension de perturbation du type en échelle échelonnée d'une forme lambda "Λ".
PCT/KR2018/005128 2017-06-29 2018-05-03 Système de supplémentation et procédé de supplémentation d'agcl pour capteur de dispositif de surveillance continue du glucose WO2019004585A1 (fr)

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KR1020170082612A KR101979257B1 (ko) 2017-06-29 2017-06-29 CGMS 센서용 AgCl 보충시스템 및 보충방법
KR10-2017-0082612 2017-06-29

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