WO2018221904A3 - 인간 성체 간세포 리프로그래밍 배지 조성물 - Google Patents

인간 성체 간세포 리프로그래밍 배지 조성물 Download PDF

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Publication number
WO2018221904A3
WO2018221904A3 PCT/KR2018/006015 KR2018006015W WO2018221904A3 WO 2018221904 A3 WO2018221904 A3 WO 2018221904A3 KR 2018006015 W KR2018006015 W KR 2018006015W WO 2018221904 A3 WO2018221904 A3 WO 2018221904A3
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WIPO (PCT)
Prior art keywords
human adult
medium composition
reprogramming medium
adult hepatocyte
hepatocyte reprogramming
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PCT/KR2018/006015
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English (en)
French (fr)
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WO2018221904A2 (ko
Inventor
최동호
정재민
김요한
강교진
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한양대학교 산학협력단
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Application filed by 한양대학교 산학협력단 filed Critical 한양대학교 산학협력단
Priority to US16/618,270 priority Critical patent/US20200147143A1/en
Publication of WO2018221904A2 publication Critical patent/WO2018221904A2/ko
Publication of WO2018221904A3 publication Critical patent/WO2018221904A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/14—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from hepatocytes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 인간 성체 간세포 리프로그래밍 배지 조성물에 관한 것으로, 본 발명은 화학물질의 조합을 통해 인간 성체 간세포로부터 간 전구세포를 유도하는 방법을 제공한다.
PCT/KR2018/006015 2017-05-29 2018-05-28 인간 성체 간세포 리프로그래밍 배지 조성물 WO2018221904A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/618,270 US20200147143A1 (en) 2017-05-29 2018-05-28 Human adult hepatocyte reprogramming medium composition

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0066120 2017-05-29
KR1020170066120A KR102107602B1 (ko) 2017-05-29 2017-05-29 인간 성체 간세포 리프로그래밍 배지 조성물

Publications (2)

Publication Number Publication Date
WO2018221904A2 WO2018221904A2 (ko) 2018-12-06
WO2018221904A3 true WO2018221904A3 (ko) 2019-04-11

Family

ID=64454830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/006015 WO2018221904A2 (ko) 2017-05-29 2018-05-28 인간 성체 간세포 리프로그래밍 배지 조성물

Country Status (3)

Country Link
US (1) US20200147143A1 (ko)
KR (1) KR102107602B1 (ko)
WO (1) WO2018221904A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110982776B (zh) * 2018-09-30 2023-07-04 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
KR20220021883A (ko) * 2020-08-14 2022-02-22 한양대학교 산학협력단 체외에서 유전자 교정된 세포 및 이의 용도
KR102504479B1 (ko) * 2020-10-12 2023-03-03 한양대학교 산학협력단 삼차원 인공 튜브형 스캐폴드 및 이의 제조 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104024401A (zh) * 2011-06-10 2014-09-03 荷兰皇家科学院 用于干细胞的培养基
CN105154386A (zh) * 2014-05-30 2015-12-16 中国人民解放军第二军医大学东方肝胆外科医院 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法
KR20160047523A (ko) * 2013-08-28 2016-05-02 프로메테라 바이오사이언시즈 에스.에이./엔.브이. 성체 간 전구 세포 제조 방법
US20160237400A1 (en) * 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102741394B (zh) * 2009-10-16 2016-05-04 斯克里普斯研究所 多能细胞的诱导
CN109890956A (zh) * 2016-10-28 2019-06-14 国立研究开发法人国立癌研究中心 人肝前体细胞的制备方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104024401A (zh) * 2011-06-10 2014-09-03 荷兰皇家科学院 用于干细胞的培养基
US20160237400A1 (en) * 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
KR20160047523A (ko) * 2013-08-28 2016-05-02 프로메테라 바이오사이언시즈 에스.에이./엔.브이. 성체 간 전구 세포 제조 방법
CN105154386A (zh) * 2014-05-30 2015-12-16 中国人民解放军第二军医大学东方肝胆外科医院 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KATSUDA, TAKESHI ET AL.: "Conversion of Terminally Committed Hepatocytes to Culturable Bipotent Progenitor Cells with Regenerative Capacity", CELL STEM CELL, vol. 20, no. 1, 2 January 2017 (2017-01-02), pages 41 - 55, XP055397277, DOI: doi:10.1016/j.stem.2016.10.007 *

Also Published As

Publication number Publication date
KR20180130625A (ko) 2018-12-10
US20200147143A1 (en) 2020-05-14
KR102107602B1 (ko) 2020-05-08
WO2018221904A2 (ko) 2018-12-06

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