WO2018135843A2 - Souche de lactobacillus fermentum pouvant empêcher la perte de cheveux, favoriser la pousse des cheveux, ou améliorer la fonction sexuelle, et composition comprenant cette souche - Google Patents

Souche de lactobacillus fermentum pouvant empêcher la perte de cheveux, favoriser la pousse des cheveux, ou améliorer la fonction sexuelle, et composition comprenant cette souche Download PDF

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WO2018135843A2
WO2018135843A2 PCT/KR2018/000757 KR2018000757W WO2018135843A2 WO 2018135843 A2 WO2018135843 A2 WO 2018135843A2 KR 2018000757 W KR2018000757 W KR 2018000757W WO 2018135843 A2 WO2018135843 A2 WO 2018135843A2
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composition
strain
lactobacillus
kccm11910p
ceb
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WO2018135843A3 (fr
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염규진
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(주)코엔바이오
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/143—Fermentum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus

Definitions

  • the present invention relates to a novel strain having a hair loss prevention, promoting hair growth or improving sexual function and a composition comprising the same.
  • the cause of hair loss is not well known yet, but there are reports of possible causes such as genetic factors, hormonal abnormalities, and immunological abnormalities.
  • the present invention is to provide a novel lactic acid bacteria strain effective for preventing hair loss, promoting hair growth or improving sexual function.
  • the present invention is to provide a composition for preventing hair loss, promoting hair growth or improving sexual function that can be safely used without side effects.
  • the novel Lactobacillus fermentum ( Lactobacillus fermentum ) Provides a KCCM11910P strain.
  • the morphological characteristics of the strain are Gram-positive bacteria, have a nucleotide sequence of SEQ ID NO: 1, and have 99% homology with Lactobacillus percentage as analyzed by 16s rRNA partial sequencing. Accordingly, the present inventors deposited the newly isolated Lactobacillus pertumtum KCCM11910P at the Korean Culture Center of Microorganisms (120-861, Hongje-2ga-gil, Seodaemun-gu, Seoul, Korea) on October 7, 2016. The strain is isolated from the traditional Korean food Cheonggukjang. The strain is effective in preventing hair loss, promoting hair growth, improving sexual function, lowering cholesterol, and / or lipolysis.
  • Another aspect of the invention relates to a culture of Lactobacillus permanent KCCM11910P, concentrates thereof, or dried products thereof.
  • the novel isolated strain of the present invention can be cultured through a conventional method for culturing Bacillus or Lactobacillus genus.
  • the medium may be natural or synthetic medium.
  • the carbon source of the medium for example, glucose, sucrose, dextrin, glycerol, starch and the like can be used, and as the nitrogen source, peptone, meat extract, yeast extract, dried yeast, soybean, ammonium salt, nitrate and other organic or inorganic Nitrogen containing compounds may be used, but are not limited to these components.
  • Inorganic salts contained in the medium include magnesium. Manganese, calcium. Iron, potassium and the like can be used, but are not limited thereto.
  • culture temperature conditions of the newly isolated strain of the present application can be incubated for 12 hours to 4 days in the temperature range of 20 ⁇ 40 °C.
  • medicinal preparations such as seoritae, baekshou, sukjihwang, licorice, cheongung, tofu, cinnamon, donkey, Seokpo, red sesame, ephedra, ginger, white porcelain, cheoncho, horseback riding, mandrel, etc.
  • the culture of the newly isolated strain may be a culture stock solution including the cells, and may also be a culture cell from which the culture supernatant is removed or concentrated.
  • the culture composition may further include components necessary for culturing Bacillus or Lactobacillus strains, as well as components that act synergistically to the growth of Bacillus or Lactobacillus strains, the composition according to the conventional techniques in the art It can be easily selected by those who have it.
  • the state of the strain may be a liquid state or dry state
  • the drying method is possible, but not limited to ventilation drying, natural drying, spray drying and freeze drying.
  • the present invention relates to a composition
  • a composition comprising the Lactobacillus permanent strain of the present invention, a culture thereof, a concentrate thereof, or a dried product thereof.
  • the Lactobacillus fermentum strain may specifically be Lactobacillus fermentum KCCM11910P, but is not limited thereto.
  • the composition may be a food composition or a pharmaceutical composition for preventing hair loss, promoting hair growth, or improving sexual function, and further, has a lipolytic effect and / or a cholesterol lowering effect.
  • One aspect of the food composition may be a food, health food, dietary supplement, or health functional food composition.
  • One aspect of the pharmaceutical composition may be a quasi-drug or a pharmaceutical preparation.
  • the composition is Seoryae, Baekshou, Sukjihwang, licorice, Cheongung, tofu, cinnamon, Angelica, Seokchangpo, Red sesame, ephedra, prickly pear, pine needles, pine flower, celticus, jimo, ginger, white porcelain, cheoncho, horseback riding, bay
  • Herbal ingredients effective for preventing hair loss or promoting hair growth such as herbal preparations such as a typewriter, extracts thereof, or mixtures thereof; Or may further comprise a pharmaceutical component such as minoxidil or finasteride.
  • the composition is Brevibacillus Genus , Lactobacillus Genus, Lactococcus Genus, Propionibacterium Genus, Enterocuccus Genus, Strains such as the Bifidobacterium genus may be further included.
  • Brevibacillus reuszeri E.g. KCCM11911P
  • Enterococcus faecium e.g. KCCM11909P
  • Leuconostoc mesenteroides e.g. KCCM11827P
  • leukonostock holzafel e.g. KCCM11830P
  • Lactobacillus sakei e.g. KCCM11841P
  • Culture or mixtures thereof.
  • composition may further comprise collagen.
  • the composition of the present invention comprises Lactobacillus fermentum (eg KCCM11910P) strains of 5 x 10 4 CFU / ml to 5 x 10 10 CFU / ml, preferably 5 x 10 4 CFU / ml to 5 x 10 8 LFU Bacillus percentages of CFU / ml (eg KCCM11910P).
  • the composition may be administered 30ml to 100ml per dose, once to four times a day at the above concentration.
  • composition of the present invention may further include a pharmaceutically or food acceptable carrier, and may be formulated with the carrier to be provided as a food or medicine.
  • the term "pharmaceutically or food acceptable carrier” refers to a carrier or diluent that does not stimulate the organism and does not inhibit the biological activity and properties of the administered strain.
  • composition may be formulated in a variety of oral or parenteral formulations, but may preferably be formulated in a liquid oral solution.
  • acceptable pharmaceutical or food acceptable carriers are sterile and physiologically compatible carriers, including saline, sterile water, buffered saline, albumin injectable solutions, dextrose solution, maltodextrin solution, glycerol and these
  • saline sterile and physiologically compatible carriers
  • sterile water sterile water
  • buffered saline albumin injectable solutions
  • dextrose solution dextrose solution
  • maltodextrin solution glycerol
  • glycerol glycerol
  • diluents, dispersants, surfactants, binders and lubricants may be additionally added to formulate into liquid formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.
  • the dosage is from 5 x 10 4 to 5 x 10 8 CFU / ml of Lactobacillus permanent (eg KCCM11910P) strain, preferably from 1 x 10 6 to 1 x 10 8 CFU / ml.
  • the amount of Lactobacillus permanent eg KCCM11910P may be administered from 30ml to 100ml per dose once to four times a day.
  • 50 ml per dose may be administered 2 to 4 times per day in an amount of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage (eg KCCM11910P). More specifically, the amount of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage (eg, KCCM11910P) may be administered once to twice daily with 50ml to 100ml per dose. More specifically, 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage (eg KCCM11910P) is administered once 50ml to 100ml 30 minutes before breakfast, and 50ml to 30 minutes before dinner or before bedtime. 100 ml can be administered once.
  • 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage eg KCCM11910P
  • 100 ml can be administered once.
  • Formulations for oral administration comprising the Lactobacillus fermentum of the present invention (e.g., KCCM11910P) include, for example, tablets, troches, lozenges, water-soluble or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, Syrups or elixirs.
  • binders such as cellulose or gelatin
  • excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, stearic acid Lubricants such as magnesium, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be included, and the capsule formulation may further contain a liquid carrier such as fatty oil in addition to the aforementioned substances.
  • the daily dosage of the composition or Lactobacillus permanent will vary depending on the patient's weight, age, sex, health condition, treatment, prevention or major symptoms, time of administration, method of administration and severity. But may be about 0.0001 mg / kg to about 10 g / kg, specifically about 0.01 mg / kg to about 500 mg / kg, and may be administered or applied once to six times a day, for example, once to four times a day. Can be.
  • the lactobacillus permanents (eg, KCCM11910P) in the composition may be in liquid or dry state, preferably in liquid form. Drying methods may be, but not limited to, ventilation drying, natural drying, spray drying and freeze drying.
  • the Lactobacillus permanentum of the present invention e.g., KCCM11910P
  • One aspect of the present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a Lactobacillus latement (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • a Lactobacillus latement eg, KCCM11910P
  • KCCM11910P Lactobacillus latement
  • Another aspect of the invention comprises administering to a subject a therapeutic or prophylactically effective amount of a Lactobacillus pertumtum (eg KCC11910P) strain or culture thereof, concentrate or dried product thereof to prevent hair loss or promote hair growth. It relates to a method for preventing hair loss or a method for treating hair loss.
  • the therapeutic or prophylactically effective amount may vary depending on the weight, age, sex, health condition, treatment, prevention, or major symptoms, time of administration, administration method, and severity of the patient, as described above.
  • Lactobacillus permanum (eg KCCM11910P) strains of 5 ⁇ 10 4 to 5 ⁇ 10 8 CFU / ml, preferably 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentagement (eg KCCM11910P 30 ml to 100 ml per dose may be administered once to four times daily. In one example, 50 ml per dose may be administered 2 to 4 times per day in an amount of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage (eg KCCM11910P).
  • the amount of 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage may be administered once to twice daily with 50ml to 100ml per dose. More specifically, 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus percentage (eg KCCM11910P) is administered once 50ml to 100ml 30 minutes before breakfast, and 50ml to 30 minutes before dinner or before bedtime. 100 ml can be administered once.
  • One aspect of the present invention relates to a composition for improving sexual dysfunction, including Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • the sexual dysfunction improvement may be, for example, an improvement in erectile dysfunction.
  • another aspect of the present invention comprises administering to a subject for the prevention or treatment of sexual dysfunction a therapeutic or prophylactically effective amount of a Lactobacillus latement (eg, KCCM11910P) strain or its culture, its concentrate or its dry matter.
  • the present invention relates to a method for preventing or treating sexual dysfunction.
  • the therapeutic or prophylactically effective amount is the same as described above.
  • One aspect of the present invention relates to a composition for lowering cholesterol, comprising a Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • a Lactobacillus fermentum eg, KCCM11910P
  • high cholesterol comprising administering to a subject a therapeutically or prophylactically effective amount of a Lactobacillus pertumtum (eg, KCCM00000) strain or culture thereof, concentrate or dried product thereof to lower cholesterol
  • a method for preventing or treating bloodemia is the same as described above.
  • One aspect of the present invention relates to a composition for lipolysis comprising a Lactobacillus pertumtum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • a composition for lipolysis comprising a Lactobacillus pertumtum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.
  • another aspect of the invention comprises administering to a subject for the prevention or treatment of hyperlipidemia a therapeutic or prophylactically effective amount of a Lactobacillus fermentum (eg, KCCM11910P) strain or culture thereof, concentrate or dried product thereof , A method of preventing or treating hyperlipidemia.
  • the therapeutic or prophylactically effective amount is the same as described above.
  • Lactobacillus fermentum strain of the present invention eg, KCCM11910P
  • a composition comprising the same is effective for preventing hair loss, promoting hair growth or improving sexual function.
  • Lactobacillus permanent eg KCCM11910P
  • a composition comprising the same can be used safely to prevent hair loss, promote hair growth, lower cholesterol or improve sexual function without side effects.
  • 1 is a hair comparison photograph before and after (about 2 months) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain.
  • KCCM11910P Lactobacillus permanent Ceb-ch-002
  • FIG. 4 is a comparison of hair before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and Brevibacillus lugeri Ceb-ch-003 (KCCM11911P).
  • FIG. 5 is a hair comparison photograph before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and Brevibacillus rugeri Ceb-ch-003 (KCCM11911P).
  • FIG. 6 is a comparison of hair before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and Brevibacillus rugeri Ceb-ch-003 (KCCM11911P).
  • Figure 7 shows Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and kimchi extract bacteria Leukonstock Holzpellelli Ceb-kc-003 ( Leuconostoc Hair comparison picture before and after taking holzapfelii Ceb-kc-003 (KCCM11830P).
  • Figure 8 is a graph showing the cholesterol lowering effect compared to Lactobacillus plantarum strain Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain and other lactic acid strains.
  • Figure 9 is a graph showing the NO production concentration compared to Lactobacillus plantarum strain Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain and other lactic acid strains.
  • Cheonggukjang a traditional Korean food, was collected, and the obtained sample was diluted in stages and incubated in BHI solid medium (Difco, USA) added with 3% sodium chloride for 24 hours at 37 ° C. The predominant strain was isolated from the sample. Selected colonies were purely separated by culturing in a new medium over 3 times, and the pure cultured cells were stored in a medium to which 20% glycerol was added and stored at below 70 ° C.
  • morphological and biochemical investigations were performed primarily.
  • the morphological features were Gram-stained and Gram-positive.
  • the analysis was performed by 16s rRNA partial sequencing to analyze the taxonomic characteristics. As a result, it was confirmed that the isolate had a nucleotide sequence of SEQ ID NO: 1, and the isolated strain had 99% homology with Lactobacillus percentage.
  • the inventors deposited the newly isolated Lactobacillus permanent Ceb-ch-002 at the Korean Culture Center of Microorganisms (120-861, Hongje-2ga-gil, Seodaemun-gu, Seoul) on October 7, 2016. Was deposited.
  • strain powder The strain was lyophilized to prepare strain powder.
  • Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain cultures obtained in Example 1 were subjected to 1 ⁇ 10 6 to 1 month to 2 months in a total of three males aged 48, 54 and 55 with alopecia. An amount of 1 ⁇ 10 8 CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) was orally administered daily between 50 ml and 100 ml twice daily in the morning and evening.
  • the number of hairs falling out after hair washing once at 10-day intervals before and after administration of the strain was 10 days, 20 days and 30 days.
  • Hair loss number before and after taking Test subject Taking date 48 year old male 54 year old male 55 year old male Before taking 55 hair loss 44 hair loss 38 hair loss 10 days after taking 36 hair loss 33 hair loss 26 hair loss 20 days after taking 21 hair loss 19 hair loss 20 hair loss 30 days after taking 12 hair loss 5 hair loss 8 hair loss
  • Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain of the present invention is effective in preventing hair loss, and is also effective in treating hair loss by remarkably improving hair thickness, quantity and shine.
  • Example 3 Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Strains Brevibacillus rugeri Ceb -ch-003 ( KCCM11911P ) Hair loss prevention effect of strain combination
  • the culture solution of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 was isolated from Cheonggukjang, and the Korean Culture Center of Microorganisms, Hongje-2ga, Seodaemun-gu, Seoul, October 7, 2016. Brevibacillus deposited with the accession number KCCM11911P at -120-861) reuszeri ) Cultures of Ceb-ch-003 (KCCM11911P) strains were administered to a total of three men 55 years, 53 years and 48 years old with alopecia for 1 to 2 months.
  • Specific dosing methods include 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) at 25 ml to 50 ml, 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml Brevibacillus reuszeri Ceb-ch-003 (KCCM11911P) 25 ml to 50 ml per dose were administered orally twice daily.
  • Hair loss number before and after taking Test subject Taking date 55 year old male 53 year old male 48 year old male Before taking 60 hair loss 55 hair loss 57 hair loss 10 days after taking 45 hair loss 43 hair loss 45 hair loss 20 days after taking 23 hair loss 27 hair loss 36 hair loss 30 days after taking 10 hair loss 7 hair loss 13 hair loss
  • Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain of the present invention is another strain, Brevibacillus rugeri ( Brevibacillus) reuszeri )
  • Ceb-ch-003 (KCCM11911P) strain When used in combination with Ceb-ch-003 (KCCM11911P) strain, it can be seen that it is more effective in preventing hair loss.
  • Example 4 Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Hair Loss Prevention Effects of Strains and Leukonostock Holzafelly Ceb-kc-003 (KCCM11830P) Strains
  • the culture medium of Lactobacillus permantum Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and the culture medium of leukonostock Holzafelly Ceb-kc-003 (KCCM11830P) strain isolated from kimchi were 66 years old with hair loss symptoms. A male was administered for 4 months.
  • Specific dosing methods include 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) at 25 ml to 50 ml, 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml Rukono Stock Holz Felice Ceb-kc-003 (KCCM11830P) 25 ml to 50 ml per dose were administered orally twice daily.
  • Hair loss before and after taking Test subject Taking date 66 year old male Before taking 45 hair loss 10 days after taking 41 hair loss 20 days after taking 20 hair loss 30 days after taking 15 hair loss
  • Example 5 Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Strains Enterococcus Paesium KCCM11909P Hair loss prevention effect of strain combination
  • Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and cultures of the Enterococcus faecium KCCM11909P strain were administered to a 60-year-old male with alopecia for 3 months.
  • Specific administration methods include 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) 25 ml to 50 ml per time, 1 ⁇ 10 6 to 1 ⁇ 10 8 CFU / ml of enterocock
  • Hair loss number before and after taking Test subject Taking date 60 year old male Before taking 45 hair loss 10 days after taking 42 hair loss 20 days after taking 27 hair loss 30 days after taking 23 hair loss
  • Lactobacillus Permanent Ceb-ch-002 (KCCM11910P) strain powder
  • 1 mg / ml to 5 mg / ml Lactobacillus Permanent Ceb-ch-002 (KCCM11910P) lyophilized powder solution
  • 1 mg / ml to 5 mg / ml Lactobacillus plantarum powder solution was used as a control, respectively.
  • the cholesterol removal ability defined in the present invention can be specifically measured according to the following method:
  • Lactobacillus permanent Ceb-ch-002 Lactobacillus plantarum 1mg / ml 21% 11% 2mg / ml 27% 16% 3mg / ml 39% 22% 4mg / ml 51% 31% 5mg / ml 62% 35%
  • Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain powder of the present invention is effective in lowering cholesterol in proportion to the concentration, which is significantly higher than other lactobacillus Lactobacillus plantarum . .
  • Example 7 Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Improvement of sexual function of strain And prevention effect
  • a total of 5 males aged 50 to 70 who are smokers or non-smokers with or without sexual dysfunction are 1 x 10 6 to 1 x 10 8 CFU / ml of Lactobacillus latex Ceb-ch-002 (KCCM11910P). ) was orally administered once daily to twice daily in an amount of 50 ml to 100 ml per time. Before and after the administration of the strain was allowed to self-evaluate the number of dawn erection, erection, erection persistence at monthly intervals.
  • Lactobacillus latent Ceb-ch-002 (KCCM11910P) strain powder of the present invention can be confirmed that there is a prophylactic effect of sexual dysfunction, and further, the therapeutic effect on sexual dysfunction.
  • Lactobacillus permanent Ceb-ch-002 Lactobacillus plantarum 1 ⁇ g / ml 3.7 ( ⁇ M) 2.5 ( ⁇ M) 10 ⁇ g / ml 4.0 ( ⁇ M) 2.9 ( ⁇ M) 20 ⁇ g / ml 4.6 ( ⁇ M) 3.1 ( ⁇ M) 30 ⁇ g / ml 5.2 ( ⁇ M) 3.3 ( ⁇ M) 40 ⁇ g / ml 6.4 ( ⁇ M) 3.5 ( ⁇ M) 50 ⁇ g / ml 7.1 ( ⁇ M) 3.5 ( ⁇ M)

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Abstract

L'invention concerne une nouvelle souche ayant pouvant empêcher la perte de cheveux, favoriser la pousse des cheveux, ou améliorer la fonction sexuelle; et une composition comprenant cette souche.
PCT/KR2018/000757 2017-01-19 2018-01-16 Souche de lactobacillus fermentum pouvant empêcher la perte de cheveux, favoriser la pousse des cheveux, ou améliorer la fonction sexuelle, et composition comprenant cette souche WO2018135843A2 (fr)

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KR1020170009281A KR101791088B1 (ko) 2017-01-19 2017-01-19 탈모 예방, 발모 촉진 또는 성기능 개선능이 있는 락토바실러스 퍼멘텀 균주 및 이를 포함하는 조성물
KR10-2017-0009281 2017-01-19

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WO2018135843A2 true WO2018135843A2 (fr) 2018-07-26
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WO2020096059A1 (fr) * 2018-11-09 2020-05-14 ニチニチ製薬株式会社 Agent externe pour la pousse des cheveux ou la prévention de la perte des cheveux
CN113797137A (zh) * 2021-10-29 2021-12-17 浙江宜格企业管理集团有限公司 一种具有滋养强韧眉毛功能的何首乌提取物及制备方法

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CN113164531B (zh) * 2018-11-09 2023-12-15 日本尼奇制药株式会社 用于生发或防止脱发的外用剂
JP7426100B2 (ja) 2018-11-09 2024-02-01 ニチニチ製薬株式会社 育毛用又は脱毛防止用外用剤
CN113797137A (zh) * 2021-10-29 2021-12-17 浙江宜格企业管理集团有限公司 一种具有滋养强韧眉毛功能的何首乌提取物及制备方法
CN113797137B (zh) * 2021-10-29 2024-04-16 浙江宜格企业管理集团有限公司 一种具有滋养强韧眉毛功能的何首乌提取物及制备方法

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