WO2018135843A2 - Lactobacillus fermentum strain having ability to prevent hair loss, promote hair growth, or improve sexual function and composition comprising same - Google Patents

Abstract

The present invention relates to a novel strain having an ability to prevent hair loss, promote hair growth, or improve sexual function, and to a composition comprising the same.

Description

Lactobacillus fermentum strain having the ability to prevent hair loss, promote hair growth or improve sexual function, and a composition comprising the same

The present invention relates to a novel strain having a hair loss prevention, promoting hair growth or improving sexual function and a composition comprising the same.

Hair loss is rapidly increasing in modern society, 60% of hair care subjects are in the 20s and 30s, and the age of hair loss is getting lower and the number of patients suffering from alopecia is estimated to reach almost 10 million. Socioeconomic problems are seriously emerging.

The more severe the hair loss, the more psychological complaints of depression and anxiety symptoms, the higher the stress perception, the difficulty of adaptation in human or heterosexual relationship, and the adjustment in the home is reported as a health concern.

The cause of hair loss is not well known yet, but there are reports of possible causes such as genetic factors, hormonal abnormalities, and immunological abnormalities.

Currently, the hair loss treatments are approved by the US Food and Drug Administration (FDA), and the transdermal application of minoxidil (Minoxidil) and orally administered Finasteride is used, but the associated side effects such as cardiovascular disorders, skin irritation, decreased sexual function and severe malformation It is showing its limitations for such reasons.

Meanwhile, previous studies have reported on the effect of improving alopecia using herbal medicines, but the results are inadequate. Recently, herbal extracts such as plant extracts and sewage were extracted with hot water and fermented with lactic acid bacteria of Lactobacillus genus. Although some patents have been reported, most of them have been applied to rats, and no studies have been applied to human hair loss or hair loss in progress.

Accordingly, the present invention is to provide a novel lactic acid bacteria strain effective for preventing hair loss, promoting hair growth or improving sexual function.

Furthermore, the present invention is to provide a composition for preventing hair loss, promoting hair growth or improving sexual function that can be safely used without side effects.

One aspect of the invention, the novel Lactobacillus fermentum ( Lactobacillus fermentum ) Provides a KCCM11910P strain. The morphological characteristics of the strain are Gram-positive bacteria, have a nucleotide sequence of SEQ ID NO: 1, and have 99% homology with Lactobacillus percentage as analyzed by 16s rRNA partial sequencing. Accordingly, the present inventors deposited the newly isolated Lactobacillus pertumtum KCCM11910P at the Korean Culture Center of Microorganisms (120-861, Hongje-2ga-gil, Seodaemun-gu, Seoul, Korea) on October 7, 2016. The strain is isolated from the traditional Korean food Cheonggukjang. The strain is effective in preventing hair loss, promoting hair growth, improving sexual function, lowering cholesterol, and / or lipolysis.

Another aspect of the invention relates to a culture of Lactobacillus permanent KCCM11910P, concentrates thereof, or dried products thereof.

The novel isolated strain of the present invention can be cultured through a conventional method for culturing Bacillus or Lactobacillus genus. The medium may be natural or synthetic medium. As the carbon source of the medium, for example, glucose, sucrose, dextrin, glycerol, starch and the like can be used, and as the nitrogen source, peptone, meat extract, yeast extract, dried yeast, soybean, ammonium salt, nitrate and other organic or inorganic Nitrogen containing compounds may be used, but are not limited to these components. Inorganic salts contained in the medium include magnesium. Manganese, calcium. Iron, potassium and the like can be used, but are not limited thereto. In addition to the components of the carbon source, nitrogen source and inorganic salts, amino acids, vitamins, nucleic acids and related compounds may be added to the medium. Culture temperature conditions of the newly isolated strain of the present application can be incubated for 12 hours to 4 days in the temperature range of 20 ~ 40 ℃. In one embodiment, medicinal preparations such as seoritae, baekshou, sukjihwang, licorice, cheongung, tofu, cinnamon, donkey, Seokpo, red sesame, ephedra, ginger, white porcelain, cheoncho, horseback riding, mandrel, etc. It is possible to further improve the effects of preventing hair loss, promoting hair growth, and improving sexual function of the strain by adding a mixture thereof and culturing and using this culture. In particular, it is preferable to add frosted and / or white sewage to the culture medium, and the amount thereof to be added is preferably 0.01 to 10% by weight, specifically 0.01 to 5% by weight, based on the weight of the medium, respectively.

The culture of the newly isolated strain may be a culture stock solution including the cells, and may also be a culture cell from which the culture supernatant is removed or concentrated. The culture composition may further include components necessary for culturing Bacillus or Lactobacillus strains, as well as components that act synergistically to the growth of Bacillus or Lactobacillus strains, the composition according to the conventional techniques in the art It can be easily selected by those who have it.

In addition, the state of the strain may be a liquid state or dry state, the drying method is possible, but not limited to ventilation drying, natural drying, spray drying and freeze drying.

In another aspect, the present invention relates to a composition comprising the Lactobacillus permanent strain of the present invention, a culture thereof, a concentrate thereof, or a dried product thereof. The Lactobacillus fermentum strain may specifically be Lactobacillus fermentum KCCM11910P, but is not limited thereto. The composition may be a food composition or a pharmaceutical composition for preventing hair loss, promoting hair growth, or improving sexual function, and further, has a lipolytic effect and / or a cholesterol lowering effect. One aspect of the food composition may be a food, health food, dietary supplement, or health functional food composition. One aspect of the pharmaceutical composition may be a quasi-drug or a pharmaceutical preparation.

In one embodiment, the composition is Seoryae, Baekshou, Sukjihwang, licorice, Cheongung, tofu, cinnamon, Angelica, Seokchangpo, Red sesame, ephedra, prickly pear, pine needles, pine flower, celticus, jimo, ginger, white porcelain, cheoncho, horseback riding, bay Herbal ingredients effective for preventing hair loss or promoting hair growth, such as herbal preparations such as a typewriter, extracts thereof, or mixtures thereof; Or may further comprise a pharmaceutical component such as minoxidil or finasteride.

In another embodiment, the composition is Brevibacillus Genus , Lactobacillus Genus, Lactococcus Genus, Propionibacterium Genus, Enterocuccus Genus, Strains such as the Bifidobacterium genus may be further included. Specifically, Brevibacillus reuszeri (E.g. KCCM11911P), Enterococcus faecium (e.g. KCCM11909P), Leuconostoc mesenteroides (e.g. KCCM11827P), leukonostock holzafel (e.g. KCCM11830P), Lactobacillus sakei (e.g. KCCM11841P), or Culture, or mixtures thereof. More specifically, Brevibacillus reuszeri (E.g. KCCM11911P), Enterococcus faecium (e.g., KCCM11909P), leukonostock holzpellelli Ceb-kc-003 (KCCM11830P), leukonostock mesenteroides KCCM11827P, Lactobacillus sakei KCCM11841P, or cultures thereof, or mixtures thereof. . In addition, the composition may further comprise collagen.

The composition of the present invention comprises Lactobacillus fermentum (eg KCCM11910P) strains of 5 x 10 ⁴ CFU / ml to 5 x 10 ¹⁰ CFU / ml, preferably 5 x 10 ⁴ CFU / ml to 5 x 10 ⁸ LFU Bacillus percentages of CFU / ml (eg KCCM11910P). The composition may be administered 30ml to 100ml per dose, once to four times a day at the above concentration.

The composition of the present invention may further include a pharmaceutically or food acceptable carrier, and may be formulated with the carrier to be provided as a food or medicine.

As used herein, the term "pharmaceutically or food acceptable carrier" refers to a carrier or diluent that does not stimulate the organism and does not inhibit the biological activity and properties of the administered strain.

The composition may be formulated in a variety of oral or parenteral formulations, but may preferably be formulated in a liquid oral solution.

When formulated in a liquid solution, acceptable pharmaceutical or food acceptable carriers are sterile and physiologically compatible carriers, including saline, sterile water, buffered saline, albumin injectable solutions, dextrose solution, maltodextrin solution, glycerol and these One or more components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to formulate into liquid formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. In addition, binders, emulsifiers, preservatives, and the like may be further added to prevent degradation of the composition, or amino acids, vitamins, enzymes, flavoring agents, nonprotein nitrogen compounds, silicates, buffers, extractants, Oligosaccharides and the like may be further included. When administered in liquid form, the dosage is from 5 x 10 ⁴ to 5 x 10 ⁸ CFU / ml of Lactobacillus permanent (eg KCCM11910P) strain, preferably from 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml. The amount of Lactobacillus permanent (eg KCCM11910P) may be administered from 30ml to 100ml per dose once to four times a day. In one example, 50 ml per dose may be administered 2 to 4 times per day in an amount of 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentage (eg KCCM11910P). More specifically, the amount of 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentage (eg, KCCM11910P) may be administered once to twice daily with 50ml to 100ml per dose. More specifically, 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentage (eg KCCM11910P) is administered once 50ml to 100ml 30 minutes before breakfast, and 50ml to 30 minutes before dinner or before bedtime. 100 ml can be administered once.

Formulations for oral administration comprising the Lactobacillus fermentum of the present invention (e.g., KCCM11910P) include, for example, tablets, troches, lozenges, water-soluble or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, Syrups or elixirs. For formulation into tablets and capsules, such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, binders such as cellulose or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, stearic acid Lubricants such as magnesium, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be included, and the capsule formulation may further contain a liquid carrier such as fatty oil in addition to the aforementioned substances.

The daily dosage of the composition or Lactobacillus permanent (eg KCCM11910P) will vary depending on the patient's weight, age, sex, health condition, treatment, prevention or major symptoms, time of administration, method of administration and severity. But may be about 0.0001 mg / kg to about 10 g / kg, specifically about 0.01 mg / kg to about 500 mg / kg, and may be administered or applied once to six times a day, for example, once to four times a day. Can be.

The lactobacillus permanents (eg, KCCM11910P) in the composition may be in liquid or dry state, preferably in liquid form. Drying methods may be, but not limited to, ventilation drying, natural drying, spray drying and freeze drying. The Lactobacillus permanentum of the present invention (e.g., KCCM11910P) is in powder form in an amount of 0.05 to 80% by weight, for example 0.1 to 70% by weight, 0.1 to 50% by weight, or 1 to 30% by weight of the composition. Can be mixed.

One aspect of the present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a Lactobacillus latement (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof.

Another aspect of the invention comprises administering to a subject a therapeutic or prophylactically effective amount of a Lactobacillus pertumtum (eg KCC11910P) strain or culture thereof, concentrate or dried product thereof to prevent hair loss or promote hair growth. It relates to a method for preventing hair loss or a method for treating hair loss. The therapeutic or prophylactically effective amount may vary depending on the weight, age, sex, health condition, treatment, prevention, or major symptoms, time of administration, administration method, and severity of the patient, as described above. About 0.0001 mg / kg to about 10 g / kg, specifically about 0.01 mg / kg to about 500 mg / kg, and may be administered or applied once to six times a day, for example, once to four times a day. . Or, Lactobacillus permanum (eg KCCM11910P) strains of 5 × 10 ⁴ to 5 × 10 ⁸ CFU / ml, preferably 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentagement (eg KCCM11910P 30 ml to 100 ml per dose may be administered once to four times daily. In one example, 50 ml per dose may be administered 2 to 4 times per day in an amount of 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentage (eg KCCM11910P). More specifically, the amount of 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentage (eg, KCCM11910P) may be administered once to twice daily with 50ml to 100ml per dose. More specifically, 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus percentage (eg KCCM11910P) is administered once 50ml to 100ml 30 minutes before breakfast, and 50ml to 30 minutes before dinner or before bedtime. 100 ml can be administered once.

One aspect of the present invention relates to a composition for improving sexual dysfunction, including Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof. The sexual dysfunction improvement may be, for example, an improvement in erectile dysfunction. Or another aspect of the present invention comprises administering to a subject for the prevention or treatment of sexual dysfunction a therapeutic or prophylactically effective amount of a Lactobacillus latement (eg, KCCM11910P) strain or its culture, its concentrate or its dry matter. The present invention relates to a method for preventing or treating sexual dysfunction. The therapeutic or prophylactically effective amount is the same as described above.

One aspect of the present invention relates to a composition for lowering cholesterol, comprising a Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof. Or another aspect of the invention, high cholesterol, comprising administering to a subject a therapeutically or prophylactically effective amount of a Lactobacillus pertumtum (eg, KCCM00000) strain or culture thereof, concentrate or dried product thereof to lower cholesterol It relates to a method for preventing or treating bloodemia. The therapeutic or prophylactically effective amount is the same as described above.

One aspect of the present invention relates to a composition for lipolysis comprising a Lactobacillus pertumtum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof. Or another aspect of the invention comprises administering to a subject for the prevention or treatment of hyperlipidemia a therapeutic or prophylactically effective amount of a Lactobacillus fermentum (eg, KCCM11910P) strain or culture thereof, concentrate or dried product thereof , A method of preventing or treating hyperlipidemia. The therapeutic or prophylactically effective amount is the same as described above.

Lactobacillus fermentum strain of the present invention (eg, KCCM11910P) strain or a composition comprising the same is effective for preventing hair loss, promoting hair growth or improving sexual function.

Lactobacillus permanent (eg KCCM11910P) or a composition comprising the same, can be used safely to prevent hair loss, promote hair growth, lower cholesterol or improve sexual function without side effects.

1 is a hair comparison photograph before and after (about 2 months) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain.

2 is a hair comparison photograph before and after (about 2 months) taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain.

3 is a hair comparison photograph before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain (about 45 days).

FIG. 4 is a comparison of hair before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and Brevibacillus lugeri Ceb-ch-003 (KCCM11911P).

FIG. 5 is a hair comparison photograph before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and Brevibacillus rugeri Ceb-ch-003 (KCCM11911P).

FIG. 6 is a comparison of hair before and after taking Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and Brevibacillus rugeri Ceb-ch-003 (KCCM11911P).

Figure 7 shows Lactobacillus permanent Ceb-ch-002 (KCCM11910P) and kimchi extract bacteria Leukonstock Holzpellelli Ceb-kc-003 ( Leuconostoc Hair comparison picture before and after taking holzapfelii Ceb-kc-003 (KCCM11830P).

Figure 8 is a graph showing the cholesterol lowering effect compared to Lactobacillus plantarum strain Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain and other lactic acid strains.

Figure 9 is a graph showing the NO production concentration compared to Lactobacillus plantarum strain Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain and other lactic acid strains.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example  One: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P Isolation and Identification of Strains

(1) Sample acquisition and strain isolation

Cheonggukjang, a traditional Korean food, was collected, and the obtained sample was diluted in stages and incubated in BHI solid medium (Difco, USA) added with 3% sodium chloride for 24 hours at 37 ° C. The predominant strain was isolated from the sample. Selected colonies were purely separated by culturing in a new medium over 3 times, and the pure cultured cells were stored in a medium to which 20% glycerol was added and stored at below 70 ° C.

(2) Morphological and taxonomic characterization

For identification of the isolated strains, morphological and biochemical investigations were performed primarily. The morphological features were Gram-stained and Gram-positive. The analysis was performed by 16s rRNA partial sequencing to analyze the taxonomic characteristics. As a result, it was confirmed that the isolate had a nucleotide sequence of SEQ ID NO: 1, and the isolated strain had 99% homology with Lactobacillus percentage.

Accordingly, the inventors deposited the newly isolated Lactobacillus permanent Ceb-ch-002 at the Korean Culture Center of Microorganisms (120-861, Hongje-2ga-gil, Seodaemun-gu, Seoul) on October 7, 2016. Was deposited.

The strain was lyophilized to prepare strain powder.

(3) Preparation of culture medium of strain

Cultures of Lactobacillus permanent Ceb-ch-002 were prepared by the following method:

After adding 2 kg of Dextrose, 1.5 kg of whole milk powder, and 0.05 kg of yeast extract to 100 liters of purified water, they were sterilized at 121 ° C for 15-30 minutes at high temperature and high pressure, and then cooled to around 35 ° C and then Lactobacillus fermentum Ceb-ch-002 0.2 to 0.4 liter inoculation in sterile state and then incubated for 2 to 3 days at around 35 ° C to obtain a culture solution of Lactobacillus fermentum Ceb-ch-002.

Example  2: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Hair Loss Prevention Effect

Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain cultures obtained in Example 1 were subjected to 1 × 10 ⁶ to 1 month to 2 months in a total of three males aged 48, 54 and 55 with alopecia. An amount of 1 × 10 ⁸ CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) was orally administered daily between 50 ml and 100 ml twice daily in the morning and evening.

The number of hairs falling out after hair washing once at 10-day intervals before and after administration of the strain was 10 days, 20 days and 30 days.

The experimental results are shown in Table 1 below:

Hair loss number before and after taking

Test subject Taking date	48 year old male	54 year old male	55 year old male
Before taking	55 hair loss	44 hair loss	38 hair loss
10 days after taking	36 hair loss	33 hair loss	26 hair loss
20 days after taking	21 hair loss	19 hair loss	20 hair loss
30 days after taking	12 hair loss	5 hair loss	8 hair loss

In addition, in the men before and after the administration of the strain of the present invention, at a specific time point, was taken with a picture to compare the thickness, amount, glossiness, etc. of the hair, a part of the results are shown in Figures 1 to 3 have.

From the above results, it can be seen that the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain of the present invention is effective in preventing hair loss, and is also effective in treating hair loss by remarkably improving hair thickness, quantity and shine.

Example  3: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Strains Brevibacillus rugeri Ceb -ch-003 ( KCCM11911P ) Hair loss prevention effect of strain combination

The culture solution of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 was isolated from Cheonggukjang, and the Korean Culture Center of Microorganisms, Hongje-2ga, Seodaemun-gu, Seoul, October 7, 2016. Brevibacillus deposited with the accession number KCCM11911P at -120-861) reuszeri ) Cultures of Ceb-ch-003 (KCCM11911P) strains were administered to a total of three men 55 years, 53 years and 48 years old with alopecia for 1 to 2 months. Specific dosing methods include 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) at 25 ml to 50 ml, 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml Brevibacillus reuszeri Ceb-ch-003 (KCCM11911P) 25 ml to 50 ml per dose were administered orally twice daily.

The number of hairs falling out after hair washing once at 10-day intervals before and after 10, 20 and 30 days of dosing of the strains was counted.

The experimental results are shown in Table 2 below:

Hair loss number before and after taking

Test subject Taking date	55 year old male	53 year old male	48 year old male
Before taking	60 hair loss	55 hair loss	57 hair loss
10 days after taking	45 hair loss	43 hair loss	45 hair loss
20 days after taking	23 hair loss	27 hair loss	36 hair loss
30 days after taking	10 hair loss	7 hair loss	13 hair loss

In addition, in the men before and after the administration of the strain of the present invention, at a specific time point was taken with a photograph to compare the thickness, quantity, glossiness, etc. of the hair, the results of some of which are shown in Figs. have.

From the above results, the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain of the present invention is another strain, Brevibacillus rugeri ( Brevibacillus) reuszeri ) When used in combination with Ceb-ch-003 (KCCM11911P) strain, it can be seen that it is more effective in preventing hair loss.

Example  4: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Hair Loss Prevention Effects of Strains and Leukonostock Holzafelly Ceb-kc-003 (KCCM11830P) Strains

The culture medium of Lactobacillus permantum Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and the culture medium of leukonostock Holzafelly Ceb-kc-003 (KCCM11830P) strain isolated from kimchi were 66 years old with hair loss symptoms. A male was administered for 4 months. Specific dosing methods include 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) at 25 ml to 50 ml, 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml Rukono Stock Holz Felice Ceb-kc-003 (KCCM11830P) 25 ml to 50 ml per dose were administered orally twice daily.

The number of hairs falling out after hair washing once at 10-day intervals before and after 10, 20 and 30 days of dosing of the strains was counted.

The experimental results are shown in Table 3 below:

Hair loss before and after taking

Test subject Taking date	66 year old male
Before taking	45 hair loss
10 days after taking	41 hair loss
20 days after taking	20 hair loss
30 days after taking	15 hair loss

Example  5: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Strains Enterococcus Paesium KCCM11909P  Hair loss prevention effect of strain combination

Cultures of the Lactobacillus permanent Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and cultures of the Enterococcus faecium KCCM11909P strain were administered to a 60-year-old male with alopecia for 3 months. Specific administration methods include 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of Lactobacillus permanent Ceb-ch-002 (KCCM11910P) 25 ml to 50 ml per time, 1 × 10 ⁶ to 1 × 10 ⁸ CFU / ml of enterocock The Curse Paesium KCCM11909P strain was administered orally daily from 25 ml to 50 ml twice daily.

The number of hairs falling out after hair washing once at 10-day intervals before and after 10, 20 and 30 days of dosing of the strains was counted.

The experimental results are shown in Table 4 below:

Hair loss number before and after taking

Test subject Taking date	60 year old male
Before taking	45 hair loss
10 days after taking	42 hair loss
20 days after taking	27 hair loss
30 days after taking	23 hair loss

Example  6: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P Cholesterol-lowering Effect of

In order to examine the cholesterol lowering effect of Lactobacillus Permanent Ceb-ch-002 (KCCM11910P) strain powder, 1 mg / ml to 5 mg / ml Lactobacillus Permanent Ceb-ch-002 (KCCM11910P) lyophilized powder solution, And 1 mg / ml to 5 mg / ml Lactobacillus plantarum powder solution was used as a control, respectively.

<Cholesterol removal ability>

The cholesterol removal ability defined in the present invention can be specifically measured according to the following method:

30 ml of MRS culture paper is placed in a 50 ml falcon tube, and the test bacteria (glycerol stock) are inoculated (1 × 10 ⁷ / ml) and incubated at 30 ° C. for 24 hours. 300 μl of the obtained culture was added to 30 ml of cholesterol culture medium (350 mg / L cholesterol (Wako Pure Chemical), MRS culture medium containing 0.2% bile acid (w / ｖ). , Centrifugal separation at 4 ° C., 8,000 rpm for 10 minutes, to determine the cholesterol level in the supernatant using Determiner FC (Kyowa Medex Co., Ltd.). The ratio (%) of cholesterol removed from the culture to the amount of cholesterol contained (cholesterol) is calculated.

The results are shown in Figure 8 and Table 5, which is shown as the average value after three iterations.

	Lactobacillus permanent Ceb-ch-002 (KCCM11910P)	Lactobacillus plantarum
1mg / ml	21%	11%
2mg / ml	27%	16%
3mg / ml	39%	22%
4mg / ml	51%	31%
5mg / ml	62%	35%

From the above results, it can be seen that the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain powder of the present invention is effective in lowering cholesterol in proportion to the concentration, which is significantly higher than other lactobacillus Lactobacillus plantarum . .

Example  7: Lactobacillus Permanent Ceb -ch-002 ( KCCM11910P ) Improvement of sexual function of strain And prevention effect

A total of 5 males aged 50 to 70 who are smokers or non-smokers with or without sexual dysfunction are 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml of Lactobacillus latex Ceb-ch-002 (KCCM11910P). ) Was orally administered once daily to twice daily in an amount of 50 ml to 100 ml per time. Before and after the administration of the strain was allowed to self-evaluate the number of dawn erection, erection, erection persistence at monthly intervals.

A total of 3 people answered the questionnaire during the 1 to 3 months of dosing, and the results are shown in Table 6 below:

Sexual function improvement effect data

Test subject Dose duration	51 year old male (Non-smoker)	54 year old male (Non-smoker)	65 year old male (smoker)
Before taking	Good erection	Low erection		Erection difficulty
1 month after taking	Increased erection endurance	Dawn erection		Dawn erection
2 months after taking	Increased number of erections	Dawn Erection Increase	Increased erection endurance
3 months after taking	Increased number of erections and lasting erection	Increased erection endurance	Increased number of erections

From the above results, the Lactobacillus latent Ceb-ch-002 (KCCM11910P) strain powder of the present invention can be confirmed that there is a prophylactic effect of sexual dysfunction, and further, the therapeutic effect on sexual dysfunction.

In addition, the 24 hour production concentration of NO (from Hacat cells), which helps improve erectile power by increasing blood flow and capillary relaxation, was further evaluated. Comparative results (average value after three iterations) between the new strain Ceb-ch-002 (KCCM11910P) and Lactobacillus plantarum of the present invention are shown in FIG. 9 and Table 7. FIG.

	Lactobacillus permanent Ceb-ch-002 (KCCM11910P)	Lactobacillus plantarum
1 μg / ml	3.7 (μM)	2.5 (μM)
10 μg / ml	4.0 (μM)	2.9 (μM)
20 μg / ml	4.6 (μM)	3.1 (μM)
30 μg / ml	5.2 (μM)	3.3 (μM)
40 μg / ml	6.4 (μM)	3.5 (μM)
50 μg / ml	7.1 (μM)	3.5 (μM)

Claims (17)

1. Lactobacillus fermentum Ceb-ch-002 Ceb-ch-002 (KCCM11910P) strain.
2. The strain according to claim 1, wherein the strain is for use in preventing hair loss, promoting hair growth, improving sexual function, lowering cholesterol or lipolysis.
3. Culture of Lactobacillus permanent Ceb-ch-002 (KCCM11910P), concentrates thereof, or dried products thereof.
4. A composition for preventing hair loss, promoting hair growth, improving sexual function, lowering cholesterol or lipolysis, comprising the Lactobacillus fermentum strain, its culture, its concentrate, or its dried product.
5. The composition of claim 4, wherein the Lactobacillus fermentum strain is Lactobacillus fermentum Ceb-ch-002 (KCCM11910P).
6. The composition of claim 4, wherein the composition is for preventing hair loss, promoting hair growth, or improving sexual function.
7. The composition of claim 4 wherein the composition is a pharmaceutical composition or a food composition.
8. The composition of any one of claims 4 to 7, wherein the composition is administered in oral or parenteral form.
9. 8. The composition of any one of claims 4 to 7, wherein the Lactobacillus permanent strain is administered in an amount of about 0.0001 mg / kg to about 10 g / kg per day.
10. The method according to any one of claims 4 to 7, wherein the Lactobacillus permanent strain is administered at a concentration of 5 x 10 ⁴ to 5 x 10 ⁸ CFU / ml 30 ml to 100 ml per dose, once to four times daily. Composition.
11. 8. The composition of claim 4, wherein the composition further comprises a pharmaceutically or food acceptable carrier. 9.
12. Of claim 4 to claim 7 according to any one of the preceding claims, wherein the composition, Brevibacillus genus, Leuconostoc genus, genus Lactobacillus, genus Lactococcus, genus Propionibacterium, in Enterocuccus, or Further comprising a strain of the genus Bifidobacterium .
13. The method of claim 12, wherein the composition is, Leuconostoc genus, Brevibacillus genus Or the genus Enterocuccus , cultures thereof, concentrates thereof, dried products thereof, or mixtures thereof.
14. The composition of any one of claims 4 to 7, wherein the improvement in sexual function is an improvement in erectile dysfunction.
15. The composition of any one of claims 4 to 7, wherein the Lactobacillus fermentum strain is isolated in Cheonggukjang.
16. 8. The composition of claim 4, wherein the Lactobacillus permanent strain is comprised between 0.05 and 80% by weight of the weight of the composition. 9.
17. The method according to claim 1 or 2, wherein the Lactobacillus fermentum Ceb-ch-002 ( Lactobacillus fermentum Ceb-ch-002: KCCM11910P) has a nucleotide sequence of SEQ ID NO: 1.

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