KR101840376B1 - Lactobacillus fermentum stain for preventing depilation, improving hair growth or improving sexual disfunction, and composition comprising the same - Google Patents

Abstract

The present invention relates to a novel strain with hair loss prevention functions, hair growth promotion functions, or sexual function improvement ability, and a composition containing the same.

Description

Lactobacillus fermentum stain for preventing depilation, improving hair growth or improving sexual disfunction, and composition comprising the same}

The present invention relates to a novel strain capable of preventing hair loss, promoting hair growth or improving sexual function, and a composition comprising the same.

As we go to the modern society, the number of hair loss patients is increasing rapidly, and the age range of hair loss is gradually decreasing, and the number of patients suffering from alopecia is estimated to reach almost 10 million people, with 60% of hair care subjects in their 20s and 30s. The socio-economic problems that followed are seriously emerging.

The more severe the hair loss is, the more psychologically depression or anxiety symptoms are, the higher the perception of stress, difficulties in adaptation in human or heterosexual relationships, and it is reported that the adaptation in the home is not smooth, which is a health concern.

The cause of hair loss is not yet well known, but there are reports of genetic factors, hormonal abnormalities, and immunological abnormalities as possible causes.

Currently, Minoxidil for transdermal application and Finasteride for oral administration, approved by the US Food and Drug Administration (FDA), are widely used as treatments for hair loss, but concomitant side effects such as cardiovascular disorders, skin irritation, decreased sexual function, and serious malformations are caused. The limitations are revealed for reasons such as.

Meanwhile, previous studies have reported on the effect of improving alopecia using herbal medicines, but the results are inadequate. Recently, herbal materials such as plant extracts and sewage were extracted with hot water and fermented into Lactobacillus genus Lactobacillus to promote hair growth. Although some patents have been reported, most of the research results have been applied to experimental mice, and there is no research applied to people who are actually experiencing hair loss or hair loss.

Korean Patent Application Publication No. 2006-0092676 Korean Patent Application Publication No. 2006-0039670

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Accordingly, the present invention is to provide a novel lactic acid bacteria strain effective in preventing hair loss, promoting hair growth or improving sexual function.

Further, the present invention is to provide a composition for preventing hair loss, promoting hair growth, or improving sexual function that can be safely used without side effects.

One aspect of the present invention is a novel Lactobacillus fermentum KCCM11910P strain is provided. The morphological feature of the strain is Gram-positive bacteria, has a nucleotide sequence of SEQ ID NO: 1, and as a result of analysis by 16s rRNA partial sequencing, it has 99% homology with Lactobacillus fermentum. Accordingly, the present inventors deposited the newly isolated Lactobacillus fermentum KCCM11910P to the Korean Culture Center of Microorganisms (120-861 Hongje-2ga-gil, Seodaemun-gu, Seoul) on October 7, 2016 under the deposit number KCCM11910P. The strain is isolated from Korean traditional food, Cheonggukjang. The strain is effective in preventing hair loss, promoting hair growth, improving sexual function, lowering cholesterol, and/or decomposing fat.

Another aspect of the present invention relates to a culture of Lactobacillus fermentum KCCM11910P, a concentrate thereof, or a dried product thereof.

The novel isolated strain of the present invention can be cultured through a conventional method of culturing Bacillus or Lactobacillus sp. As a medium, natural or synthetic medium may be used. As the carbon source of the medium, for example, glucose, sucrose, dextrin, glycerol, starch, etc. may be used, and as the nitrogen source, peptone, meat extract, yeast extract, dried yeast, soybean, ammonium salt, nitrate and other organic or inorganic Nitrogen-containing compounds may be used, but are not limited to these components. The inorganic salt contained in the medium is magnesium. Manganese, calcium. Iron, potassium, and the like may be used, but are not limited thereto. In addition to the components of the carbon source, nitrogen source, and inorganic salt, amino acids, vitamins, nucleic acids, and compounds related thereto may be added to the medium. The culture temperature condition of the newly isolated strain of the present application can be cultured for 12 hours to 4 days in the temperature range of 20 ~ 40 ℃. In one example, in the culture medium, herbal preparations such as Seoritae, Baekhasoo, Sukjihwang, Licorice, Chungoong, Duchung, Cinnamon, Angelicae, Seokchangpo, Jeokhaesuo, Cheongbaekyeop, Ginger, Baekjain, Cheoncho, Horseback riding, Manhyeongja, extracts thereof, or By adding and culturing a mixture thereof, and using this culture, the effect of preventing hair loss, promoting hair growth, and improving sexual function of the present strain can be further improved. Particularly, it is good to add seoritae and/or white hawthorn to the culture medium, and the amount thereof added is preferably about 0.01% to 10% by weight, specifically 0.01% to 5% by weight, based on the weight of the medium, respectively.

The culture of the newly isolated strain may be a culture stock solution including cells, and may be cells obtained by removing or concentrating the culture supernatant. The composition of the culture may additionally include a component necessary for cultivating a conventional Bacillus or Lactobacillus strain, as well as a component synergistically acting on the growth of a Bacillus or Lactobacillus strain. It can be easily selected by the possessor.

In addition, the state of the strain may be a liquid state or a dry state, and the drying method may be air-dried, natural-dried, spray-dried and freeze-dried, but is not limited thereto.

In another aspect, it relates to a composition comprising the Lactobacillus fermentum strain of the present invention, a culture thereof, a concentrate thereof, or a dried product thereof. The Lactobacillus fermentum strain may be specifically Lactobacillus fermentum KCCM11910P, but is not limited thereto. The composition may be a food composition or a pharmaceutical composition for preventing hair loss, promoting hair growth, or improving sexual function, and further, has a lipolytic effect and/or a cholesterol lowering effect. One aspect of the food composition may be food, health food, health supplement food, or health functional food composition. One aspect of the pharmaceutical composition may be a quasi-drug or a pharmaceutical preparation.

In one example, the composition is Seoritae, Baekhasuoh, Sukjihwang, Licorice, Chungoong, Duchung, Cinnamon, Angelicae, Seokchangpo, Jeokhaesuoh, Chongbaekyeop, Gasiogapi, Songyeop, Songhwa, Gyomaek, Jimo, Ginger, Baekjain, Cheoncho, Horse Riding, Man Herbal ingredients effective in preventing hair loss or promoting hair growth, such as herbal preparations such as hyungja, extracts thereof, or mixtures thereof; Alternatively, it may contain additional medicinal ingredients such as minoxidil or finasteride.

In another example, the composition is Brevibacillus Genus , Lactobacillus genus, Lactococcus genus, Propionibacterium Genus, Enterocuccus genus, It may further include strains such as the genus Bifidobacterium. Specifically, Brevibacillus reuszeri (Example: KCCM11911P), Enterococcus faecium (e.g. KCCM11909P), Leuconostoc mesenteroides (e.g. KCCM11827P), Leuconostoc holjapelli (e.g. KCCM11830P), Lactobacillus sakei (e.g. KCCM11841P), or It may further comprise culture, or mixtures thereof. More specifically, Brevibacillus reuszeri (E.g. KCCM11911P), Enterococcus faecium (e.g. KCCM11909P), Leukonostock Holjapelli Ceb-kc-003 (KCCM11830P), Leukonostock Mecenteroides KCCM11827P, Lactobacillus sakei KCCM11841P, or a culture thereof, or a mixture thereof It may further include. In addition, the composition may further include collagen.

The composition of the present invention comprises a Lactobacillus fermentum (eg, KCCM11910P) strain ^{of 5 x 10 4} CFU/ml to 5 x 10 ^{10 CFU/ml, preferably 5 x 10 4} CFU/ml to 5 x 10 ⁸ CFU/ml of Lactobacillus Fermentum (eg KCCM11910P) is included. The composition may be administered at the concentration of 30ml to 100ml per time, 1 to 4 times a day.

The composition of the present invention may further include a pharmaceutically or food pharmaceutically acceptable carrier, and may be formulated with a carrier to be provided as a food or pharmaceutical product.

In the present invention, the term "pharmaceutically or food pharmaceutically acceptable carrier" refers to a carrier or diluent that does not stimulate an organism and does not inhibit the biological activity and properties of the administered strain.

The composition may be formulated in various oral or parenteral formulations, but preferably may be formulated as a liquid oral solution.

When formulated as a liquid solution, acceptable pharmaceutical or food acceptable carriers are sterilized and biocompatible, and include saline, sterile water, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, and these One or more of the components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added as necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to form liquid formulations such as aqueous solutions, suspensions, emulsions, pills, capsules, granules, or tablets. In addition, binders, emulsifiers, preservatives, etc. may be additionally added to prevent degradation of the composition, or amino acids, vitamins, enzymes, flavoring agents, non-proteinaceous nitrogen compounds, silicates, buffers, extractants, It may further include oligosaccharides and the like. When administered in a liquid form, the dosage is 5 x 10 ⁴ to 5 x 10 ⁸ CFU/ml of Lactobacillus fermentum (eg, KCCM11910P) strain, preferably 1 x 10 ⁶ to 1 x 10 ⁸ CFU/ml In the amount of Lactobacillus fermentum (eg, KCCM11910P), 30 ml to 100 ml per dose may be administered once to 4 times a day. In one example, 50 ^{ml of Lactobacillus fermentum (eg, KCCM11910P) of 1×10 6} to 1×10 ⁸ CFU/ml may be administered twice to 4 times a day. More specifically, an amount of 1 x 10 ⁶ to 1 x 10 ⁸ CFU/ml of Lactobacillus Permentum (eg, KCCM11910P) may be administered in an amount of 50 ml to 100 ml once or twice a day. More specifically, 1 x 10 ⁶ to 1 x 10 ⁸ CFU/ml of Lactobacillus fermentum (eg, KCCM11910P) is administered once 30 minutes before breakfast, 50 ml to 100 ml, 30 minutes before dinner or 50 ml before bedtime. 100ml can be administered once.

Formulations for oral administration comprising the Lactobacillus Permentum of the present invention (eg, KCCM11910P) include, for example, tablets, troches, lozenges, water-soluble or oily suspensions, powders or granules, emulsions, hard or soft capsules, Syrup or elixir. For formulation into tablets and capsules, etc., binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, stearic acid A lubricant such as magnesium, calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax may be included. In the case of a capsule formulation, a liquid carrier such as fatty oil may be further included in addition to the above-mentioned substances.

The daily dosage of the composition or Lactobacillus fermentum (e.g. KCCM11910P) varies depending on the patient's weight, age, sex, health status, major symptoms to be treated, prevented, or improved, administration time, administration method, and severity. However, it may be about 0.0001 mg/kg to about 10 g/kg, specifically about 0.01 mg/kg to about 500 mg/kg, and can be administered or applied once to 6 times a day, for example, 1 to 4 times a day. I can.

The lactobacillus fermentum (eg, KCCM11910P) in the composition may be in a liquid or dry state, preferably in a liquid form. The drying method may be air drying, natural drying, spray drying, and freeze drying, but is not limited thereto. The Lactobacillus fermentum of the present invention (e.g. KCCM11910P) is in the form of a powder in an amount of 0.05 to 80% by weight of the composition, for example, 0.1 to 70% by weight, 0.1 to 50% by weight, or 1 to 30% by weight. Can be mixed.

One aspect of the present invention relates to a composition for preventing hair loss or promoting hair growth, comprising a Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof or a dried product thereof.

Another aspect of the present invention comprises administering to a subject a therapeutically or prophylactically effective amount of a Lactobacillus fermentum (eg, KCC11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof to prevent hair loss or promote hair growth, It relates to a method of preventing hair loss or a method of treating hair loss. As described above, the therapeutically or prophylactically effective amount may vary depending on the patient's weight, age, sex, health condition, major symptoms to be treated, prevented, or improved, administration time, administration method, and severity, but 1 day. It may be about 0.0001 mg/kg to about 10 g/kg, specifically about 0.01 mg/kg to about 500 mg/kg, and may be administered or applied once to 6 times a day, such as 1 to 4 times a day. . Or, 5 x 10 ⁴ to 5 x 10 ⁸ CFU / ml of Lactobacillus fermentum (e.g., KCCM11910P) strain, preferably 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml of Lactobacillus fermentum (e.g., KCCM11910P ) In the amount of 30ml to 100ml per time can be administered once to 4 times a day. In one example, 50 ^{ml of Lactobacillus fermentum (eg, KCCM11910P) of 1×10 6} to 1×10 ⁸ CFU/ml may be administered twice to 4 times a day. More specifically, an amount of 1 x 10 ⁶ to 1 x 10 ⁸ CFU/ml of Lactobacillus Permentum (eg, KCCM11910P) may be administered in an amount of 50 ml to 100 ml once or twice a day. More specifically, 1 x 10 ⁶ to 1 x 10 ⁸ CFU/ml of Lactobacillus fermentum (eg, KCCM11910P) is administered once 30 minutes before breakfast, 50 ml to 100 ml, 30 minutes before dinner or 50 ml before bedtime. 100ml can be administered once.

One aspect of the present invention relates to a composition for improving sexual dysfunction, comprising a Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof or a dried product thereof. The improvement of sexual dysfunction may be, for example, improvement of erectile dysfunction. Or another aspect of the present invention, comprising administering a therapeutically or prophylactically effective amount of a Lactobacillus fermentum (e.g., KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dry matter thereof to a subject for the prevention or treatment of sexual dysfunction. , It relates to a method of preventing or treating sexual dysfunction. The therapeutically or prophylactically effective amount is the same as described above.

One aspect of the present invention relates to a composition for lowering cholesterol, comprising a Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof or a dried product thereof. Or in another aspect of the present invention, high cholesterol comprising administering to a subject a therapeutically or prophylactically effective amount of a Lactobacillus fermentum (e.g., KCCM00000) strain or a culture thereof, a concentrate thereof, or a dried product thereof, to lower cholesterol. It relates to a method of preventing or treating hyperemia. The therapeutically or prophylactically effective amount is the same as described above.

One aspect of the present invention relates to a composition for fat decomposition, comprising a Lactobacillus fermentum (eg, KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof. Or another aspect of the present invention, comprising administering a therapeutically or prophylactically effective amount of a Lactobacillus fermentum (e.g., KCCM11910P) strain or a culture thereof, a concentrate thereof, or a dried product thereof to a subject for the prevention or treatment of hyperlipidemia. , It relates to a method of preventing or treating hyperlipidemia. The therapeutically or prophylactically effective amount is the same as described above.

The Lactobacillus fermentum (eg, KCCM11910P) strain of the present invention or a composition comprising the same is effective in preventing hair loss, promoting hair growth, or improving sexual function.

Lactobacillus fermentum (eg, KCCM11910P) or a composition comprising the same can be safely used for preventing hair loss, promoting hair growth, lowering cholesterol or improving sexual function without side effects.

1 is a photograph of a comparison of hair before and after (about 2 months) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain.
2 is a photograph of a comparison of hair before and after (about 2 months) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain.
3 is a photograph of a comparison of hair before and after (about 45 days) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain.
4 is a photo of a comparison of hair before and after (about 45 days) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) and Brevibacillus lugeri Ceb-ch-003 (KCCM11911P).
5 is a photograph of a comparison of hair before and after (about 45 days) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) and Brevibacillus luzeri Ceb-ch-003 (KCCM11911P).
6 is a photograph of a comparison of hair before and after (about 75 days) taking Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) and Brevibacillus luzeri Ceb-ch-003 (KCCM11911P).
7 is a Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) and Kimchi extract, leukonostock whole japelli Ceb-kc-003 ( Leuconostoc This is a picture of hair comparison before and after (100 days after) taking holzapfelii Ceb-kc-003 (KCCM11830P).
Figure 8 is a graph showing the cholesterol lowering effect compared to Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain and other lactic acid bacteria Lactobacillus plantarum.
9 is a graph showing the concentration of NO production compared to the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain and another lactic acid strain, Lactobacillus plantarum.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Lactobacillus Permentum Isolation and identification of Ceb -ch -002 ( KCCM11910P) strain

(1) Sample acquisition and strain isolation

Cheonggukjang, a traditional Korean food, was collected, and the obtained sample was diluted in stages, spread on a BHI solid medium (Difco, USA) added with 3% sodium chloride, and cultured for 24 hours at 37°C. The dominant strain was isolated from the sample. The selected colonies were purely separated by transferring to a new medium for 3 times and culturing, and the pure cultured bacteria were stored in a medium to which 20% glycerol was added and stored at -70°C or less.

(2) Investigation of morphological and taxonomic characteristics

In order to identify the isolated strain, morphological and biochemical investigations were primarily performed. As a morphological feature, Gram was positive as a result of Gram staining. In order to analyze the taxonomic characteristics, analysis was performed by 16s rRNA partial sequencing, and as a result, it was confirmed to have the nucleotide sequence of SEQ ID NO: 1, and the isolated strain had 99% homology with Lactobacillus fermentum.

Accordingly, the present inventors transferred the newly isolated Lactobacillus fermentum Ceb-ch-002 to the Korean Culture Center of Microorganisms (120-861 Hongje-2ga-gil, Seodaemun-gu, Seoul) on October 7, 2016 with the deposit number KCCM11910P. Deposited with.

The strain was lyophilized to prepare a strain powder.

(3) Preparation of the culture solution of the strain

A culture solution of Lactobacillus fermentum Ceb-ch-002 was prepared in the following manner:

After adding 2 kg of Dextrose, 1.5 kg of whole milk powder, and 0.05 kg of yeast extract to 100 liters of purified water, sterilize at 121°C for 15 to 30 minutes, cool to around 35°C, and then use Lactobacillus Permentum Ceb-ch-002. 0.2 to 0.4 liters were inoculated in a sterile state, and then cultured at around 35 °C for 2 to 3 days to obtain a culture solution of Lactobacillus fermentum Ceb-ch-002.

Example 2: Lactobacillus Permentum Anti -hair loss effect of Ceb -ch-002 (KCCM11910P) strain

The Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain culture solution obtained in Example 1 was used for 1 x 10 ⁶ to 3 males aged 48, 54 and 55 years old with hair loss symptoms for 1 month to 2 months. An amount of 1×10 ⁸ CFU/ml of Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) was administered orally twice daily in the morning and evening at 50 ml to 100 ml per dose.

Before the administration of the strain and after 10, 20 and 30 days of administration, the number of hairs that fell out after washing the hair once at 10-day intervals was counted.

The experimental results are shown in Table 1 below:

Number of hair loss before and after taking Experiment subject
Date of taking 48 years old male 54 years old male 55 years old male Before taking 55 hair loss 44 hair loss 38 hair loss 10 days after taking 36 hair loss 33 hair loss 26 hair loss 20 days after taking 21 hair loss 19 hair loss 20 hair loss 30 days after taking 12 hair loss 5 hair loss 8 hair loss

In addition, in the above males, pictures were taken to compare the thickness, amount, and shine of the hair at a specific time point before and after administration of the strain of the present invention, and some results are shown in FIGS. 1 to 3. have.

From the above results, it can be seen that the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain of the present invention is effective in preventing hair loss, and is also effective in treating hair loss by remarkably improving hair thickness, quantity, and gloss.

Example 3: Lactobacillus Permentum Anti -hair loss effect of combination of Ceb-ch-002 ( KCCM11910P ) strain and Brevibacillus lugeri Ceb-ch-003 (KCCM11911P) strain

The culture solution of the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and was isolated from Cheonggukjang, and on October 7, 2016, the Korean Culture Center of Microorganisms, Hongje-2, Seodaemun-gu, Seoul -Gil 120-861) deposited with the deposit number KCCM11911P Brevibacillus reuszeri (Brevibacillus reuszeri) Ceb-ch-003 (KCCM11911P) the culture solution of the strain of 55 years old, 53 years old and 48 years old with hair loss symptoms total 3 people It was administered to men for 1 to 2 months. Specific administration method is 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml of Lactobacillus Permentum Ceb-ch-002 (KCCM11910P) 25 ml to 50 ml, 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml Brevibacillus reuszeri ) Ceb-ch-003 (KCCM11911P) It was administered orally every day from 25ml to 50ml twice a day.

Before administration of the strains and after 10, 20 and 30 days of administration, the number of hairs that fell out after washing the hair once at 10-day intervals was counted.

The experimental results are shown in Table 2 below:

Number of hair loss before and after taking Experiment subject
Date of taking 55 years old male 53 years old male 48 years old male Before taking 60 hair loss 55 hair loss 57 hair loss 10 days after taking 45 hair loss 43 hair loss 45 hair loss 20 days after taking 23 hair loss 27 hair loss 36 hair loss 30 days after taking 10 hair loss 7 hair loss 13 hair loss

In addition, in the males, pictures were taken to compare the thickness, amount, and shine of the hair at a specific time point before and after administration of the strain of the present invention, and some results are shown in FIGS. 4 to 6. have.

From the above results, the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain of the present invention is more effective in preventing hair loss when used in combination with another strain, Brevibacillus reuszeri) Ceb-ch-003 (KCCM11911P) strain. It can be seen that it is.

Example 4: Lactobacillus Permentum Anti -hair loss effect of combination of Ceb -ch-002 ( KCCM11910P) strain and Leuko Nostock Holjapelli Ceb- kc - 003 (KCCM11830P ) strain

The culture solution of the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and the culture solution of the Leukonostock Holjapelli Ceb-kc-003 (KCCM11830P) strain isolated from kimchi were used at 66 years old with hair loss symptoms. Was administered to a male of 4 months. Specific administration method is 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml of Lactobacillus Permentum Ceb-ch-002 (KCCM11910P) 25 ml to 50 ml, 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml Leukono Stock Holjap Felli Ceb-kc-003 (KCCM11830P) It was administered orally every day from 25ml to 50ml twice a day.

Before administration of the strains and after 10, 20 and 30 days of administration, the number of hairs that fell out after washing the hair once at 10-day intervals was counted.

The experimental results are shown in Table 3 below:

Number of hair loss before and after taking Experiment subject
Date of taking 66 years old male Before taking 45 hair loss 10 days after taking 41 hair loss 20 days after taking 20 hair loss 30 days after taking 15 hair loss

Example 5: Lactobacillus Permentum Ceb -ch-002 (KCCM11910P) strain and Orientation rokok coarse par when help hair loss prevention effect of the strain combination KCCM11909P

The culture solution of the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain obtained in Example 1 and the culture solution of the Enterococcus faecium KCCM11909P strain were administered to a 60-year-old male with hair loss for 3 months. Specific administration method is 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml of Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) 25 ml to 50 ml, 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml of enterococ Cus faecium KCCM11909P strain was orally administered from 25 ml to 50 ml twice a day twice a day.

Before administration of the strains and after 10, 20 and 30 days of administration, the number of hairs falling out after washing the hair once at 10 days intervals was counted.

The experimental results are shown in Table 4 below:

Number of hair loss before and after taking Experiment subject
Date of taking 60 years old male Before taking 45 hair loss 10 days after taking 42 hair loss 20 days after taking 27 hair loss 30 days after taking 23 hair loss

Example 6: Lactobacillus Permentum Ceb -ch-002 ( KCCM11910P ) Cholesterol Lowering Effect

In order to examine the cholesterol-lowering effect of the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain powder, a freeze-dried powder solution of Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) of 1 mg/ml to 5 mg/ml, respectively, And as a control, each 1mg/ml to 5mg/ml of Lactobacillus plantarum powder was used.

<Cholesterol removal ability>

The cholesterol (cholesterol) removal ability specified in the present invention can be specifically measured according to the following method:

30 ml of MRS culture paper was put into a 50 ml falcon tube, and the test bacteria (glycerol stock) were inoculated (1×10 ⁷ cells/ml), and cultured at 30° C. for 24 hours. 300 μl of the obtained culture medium is added to 30 ml of MRS culture paper containing cholesterol culture paper (350 mg/L cholesterol (Wako Pure Chemical), 0.2% bile acid (w/v)) After incubation at 37°C for 24 hours , Centrifugal separation is performed for 10 minutes at 4°C and 8,000 rpm, and the cholesterol level in the supernatant is measured using Determiner FC (Kyowa Medex Co., Ltd.). The ratio (%) of the amount of cholesterol removed from the culture medium to the amount of cholesterol contained (cholesterol) is calculated.

The results are shown in Fig. 8 and Table 5, which is expressed as an average value after repeated treatment three times.

Lactobacillus Fermentum Ceb-ch-002 (KCCM11910P) Lactobacillus plantarum 1mg/ml 21% 11% 2mg/ml 27% 16% 3mg/ml 39% 22% 4mg/ml 51% 31% 5mg/ml 62% 35%

From the above results, it can be seen that the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain powder of the present invention is effective in lowering cholesterol in proportion to the concentration, which is significantly improved compared to other lactic acid bacteria Lactobacillus plantarum. .

Example 7: Lactobacillus Permentum Ceb -ch-002 ( KCCM11910P ) Strain improvement and prevention effect of sexual function

For a total of 5 men 50 to 70 years old who are smoking or non-smokers with or without sexual dysfunction, 1 x 10 ⁶ to 1 x 10 ⁸ CFU/ml of Lactobacillus Permentum Ceb-ch-002 (KCCM11910P) for a total of 3 months ) Was administered orally once to twice daily in an amount of 50ml to 100ml per time. Before and after administration of the strain, the number of early morning erections, erections, and erectile persistence were evaluated at intervals of one month.

A total of 3 people responded to the questionnaire for 1 to 3 months of double dose, and the results are shown in Table 6 below:

Sexual function improvement effect data Experiment subject
Duration of taking 51 years old male
(Non-smoker) 54 years old male
(Non-smoker) 65 years old male
(smoker) Before taking Good erection Low erection Difficulty with erection 1 month after taking Increased erection duration Dawn erection Dawn erection 2 months after taking Increased number of erections Increased number of erections at dawn Increased erection duration 3 months after taking Increased erection recovery and erection duration Increased erection duration Increased number of erections

From the above results, it can be confirmed that the Lactobacillus fermentum Ceb-ch-002 (KCCM11910P) strain powder of the present invention has a preventive effect on sexual dysfunction, and further, has a therapeutic effect on sexual dysfunction.

In addition, the concentration of NO produced for 24 hours (occurring from Hacat cells), which helps improve erectile power by increasing blood flow and relaxing capillaries, was further evaluated. The results of a comparison test (average value after 3 repetitions) with the new strains Ceb-ch-002 (KCCM11910P) and Lactobacillus plantarum of the present invention are shown in FIGS. 9 and 7.

Lactobacillus Fermentum Ceb-ch-002 (KCCM11910P) Lactobacillus plantarum 1μg/ml 3.7(μM) 2.5(μM) 10μg/ml 4.0(μM) 2.9(μM) 20μg/ml 4.6 (μM) 3.1(μM) 30μg/ml 5.2 (μM) 3.3(μM) 40μg/ml 6.4(μM) 3.5(μM) 50μg/ml 7.1 (μM) 3.5(μM)

Korea Microorganism Conservation Center (overseas) KCCM11910P 20161007 Korea Microorganism Conservation Center (overseas) KCCM11830P 20160411 Korea Microorganism Conservation Center (overseas) KCCM11911P 20161007 Korea Microorganism Conservation Center (overseas) KCCM11909P 20161007

<110> COENBIO CO., Ltd. <120> Lactobacillus fermentum stain for preventing depilation, improving hair growth or improving sexual disfunction, and composition comprising the same <130> P16-1231 <160> 2 <170> KopatentIn 2.0 <210> 1 <211> 861 <212> DNA <213> DNA sequence of Lactobacillus fermentum KCCM11910P <400> 1 gctcaggatg aacgccggcc ggtgtgccta atacatgcaa gtcgaacgcg ttggccccaa 60 ttgattgatg gtgcttgcac ctgattgatt ttggtcgcca acgagtggcg gacgggtgag 120 taacacgtag gtaacctgcc cagaagcggg ggacaacatt tggaaacaga tgctaatacc 180 gcataacaac gttgttcgca tgaacaacgc ttaaaagatg gcttctcgct atcacttctg 240 gatggacctg cggtgcatta gcttgttggt ggggtaatgg cctaccaagg cgatgatgca 300 tagccgagtt gagagactga tcggccacaa tgggactgag acacggccca tactcctacg 360 ggaggcagca gtagggaatc ttccacaatg ggcgcaagcc tgatggagca acaccgcgtg 420 agtgaagaag ggtttcggct cgtaaagctc tgttgttaaa gaagaacacg tatgagagta 480 actgttcata cgttgacggt atttaaccag aaagtcacgg ctaactacgt gccagcagcc 540 gcggtaatac gtaggtggca agcgttatcc ggatttattg ggcgtaaaga gagtgcaggc 600 ggttttctaa gtctgatgtg aaagccttcg gcttaaccgg agaagtgcat cggaaactgg 660 ataacttgag tgcagaagag ggtagtggaa ctccatgtgt agcggtggaa tgcgtagata 720 tatggaagaa caccagtggc gaaggcggct acctggtctg caactgacgc tgagactcga 780 aagcatgggt agcgaacagg attagatacc ctggtagtcc atgccgtaaa cgatgagtgc 840 taggtgttgg agggtttccg c 861 <210> 2 <211> 868 <212> DNA <213> DNA sequence of Enterococcus faecium KCCM11909P <400> 2 gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag tcgtacgctt ctttttccac 60 cggagcttgc tccaccggaa aaagaggagt ggcgaacggg tgagtaacac gtgggtaacc 120 tgcccatcag aaggggataa cacttggaaa caggtgctaa taccgtataa caatcgaaac 180 cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg atggatggac ccgcggtgca 240 ttagctagtt ggtgaggtaa cggctcacca aggccacgat gcatagccga cctgagaggg 300 tgatcggcca cattgggact gagacacggc ccaaactcct acgggaggca gcagtaggga 360 atcttcggca atggacgaaa gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg 420 gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga gtaactgttc atcccttgac 480 ggtatctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 540 gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct taagtctgat 600 gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggagactt gagtgcagaa 660 gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag gaacaccagt 720 ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg gggagcaaac 780 aggattagat accctggtag tccacgccgt aaacgatgag tgctaagtgt tggagggttt 840 ccgcccttca gtgctgcagc taacgcat 868

Claims (10)

The concentrate or the dried product of the Lactobacillus fermentum strain, wherein the culture liquid, the concentrate or the dried product is a soybean, a soybean-derived ingredient, or a linseed Wherein the lactobacillus fermentum strain is used at a concentration of 5 x 10 &lt; ⁴ &gt; to 5 x 10 &lt; ⁸ &gt; CFU / ml in an amount of 30 ml to 100 ml per day, Orally administered food composition. The food composition according to claim 1, wherein the food composition further has a cholesterol-lowering ability. delete The food composition according to claim 1, wherein the lactobacillus fermentum strain is administered in an amount of 0.0001 mg / kg to 10 g / kg per day. The food composition according to claim 1, wherein said Lactobacillus perfumedum strain is administered at a concentration of 1 x 10 ⁶ to 1 x 10 ⁸ CFU / ml, 50 ml to 100 ml per day, twice a day. The method of claim 1, wherein the food composition, flow Pocono stock holjap pellets, Breda ratio Bacillus base jelly, Enterobacter cock coarse par when help, their culture, their concentrates, and food further comprises a counter dried form or a mixture thereof Composition. A pharmaceutical composition for promoting hair loss prevention or hair growth comprising a culture of a Lactobacillus fermentum strain, a concentrate thereof, or a dried product thereof, wherein the culture liquid, the concentrate or the dried product is selected from the group consisting of soybean, soybean- Wherein the lactobacillus fermentum strain is used at a concentration of 5 x 10 &lt; ⁴ &gt; to 5 x 10 &lt; ⁸ &gt; CFU / ml in an amount of 30 ml to 100 ml per day, A pharmaceutical composition for oral administration. 8. The pharmaceutical composition according to claim 7, wherein the lactobacillus fermentum strain comprises 0.05 to 80% by weight of the weight of the pharmaceutical composition.
The method of claim 8, wherein the pharmaceutical composition, kind Pocono stock holjap pellets, Breda ratio Bacillus base jelly, Enterobacter cock coarse par when help, their culture, its concentrate, further comprising a counter dried form or a mixture thereof, a medicament Composition. 9. The pharmaceutical composition according to claim 8, wherein the Lactobacillus perfumer strain is administered at a concentration of 1 x 10 &lt; ⁶ &gt; to 1 x 10 &lt; ⁸ &gt;

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