WO2018093069A1 - Procédé de culture 3d d'une cellule - Google Patents

Procédé de culture 3d d'une cellule Download PDF

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Publication number
WO2018093069A1
WO2018093069A1 PCT/KR2017/012196 KR2017012196W WO2018093069A1 WO 2018093069 A1 WO2018093069 A1 WO 2018093069A1 KR 2017012196 W KR2017012196 W KR 2017012196W WO 2018093069 A1 WO2018093069 A1 WO 2018093069A1
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WO
WIPO (PCT)
Prior art keywords
stem cells
microcapsules
alginic acid
cells
cell
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PCT/KR2017/012196
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English (en)
Korean (ko)
Inventor
이재영
장종욱
박중건
최고은
Original Assignee
광주과학기술원
사회복지법인 삼성생명공익재단
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Application filed by 광주과학기술원, 사회복지법인 삼성생명공익재단 filed Critical 광주과학기술원
Priority claimed from KR1020170143583A external-priority patent/KR20180054439A/ko
Publication of WO2018093069A1 publication Critical patent/WO2018093069A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Definitions

  • Microcapsules containing alginic acid is a particle having an average diameter of several tens to hundreds of micrometers containing the alginic acid polymer as a main component, means a particle having a cell for culture therein, For convenience, it can be simply described as “alginic acid microcapsules” or “alginic acid capsules”, and unless otherwise stated, all have the same meaning.
  • Figure 2 is a photograph observing cells cultured in alginic acid capsules according to an embodiment of the present invention with an optical microscope.
  • the survival rate of the stem cells is live / dead fluorescence staining, and counts the number of living cells (green) and dead cells (red) therefrom and has activity when counting. It was confirmed that the percentage of cells is maintained more than 90%.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)

Abstract

La présente invention concerne une microcapsule contenant de l'acide alginique, son procédé de préparation, un procédé de culture 3D de cellules souches utilisant ladite microcapsule, et l'utilisation de la microcapsule dans la culture de cellules souches. Dans la présente invention, des microstructures capsulaires à base d'acide alginique ou d'un acide alginique modifié par un peptide dérivé de la matrice extracellulaire permettent la culture en 3D de cellules souches et peuvent être utilisées pour une culture efficace qui conduit à une efficacité spatiotemporelle, une activité cellulaire, et une production en masse supérieures, par comparaison avec une culture 2D à caractère général dans une boîte de culture.
PCT/KR2017/012196 2016-11-15 2017-10-31 Procédé de culture 3d d'une cellule WO2018093069A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2016-0151918 2016-11-15
KR20160151918 2016-11-15
KR10-2017-0143583 2017-10-31
KR1020170143583A KR20180054439A (ko) 2016-11-15 2017-10-31 세포 3d 배양 방법

Publications (1)

Publication Number Publication Date
WO2018093069A1 true WO2018093069A1 (fr) 2018-05-24

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PCT/KR2017/012196 WO2018093069A1 (fr) 2016-11-15 2017-10-31 Procédé de culture 3d d'une cellule

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WO (1) WO2018093069A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108949524A (zh) * 2018-07-26 2018-12-07 上海大学 用于体外细胞三维微组织形成的笼结构微孔培养皿及其制备方法
CN111375361A (zh) * 2020-03-17 2020-07-07 北京唐颐惠康生物医学技术有限公司 一种用于大规模干细胞培养的纳米海藻糖3d微胶囊
CN113699094A (zh) * 2021-08-26 2021-11-26 深圳钧兴生物科技有限公司 一种无血清培养细胞的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020066507A (ko) * 2001-02-12 2002-08-19 이종원 항생제를 이용한 인공간 시스템
KR20090109127A (ko) * 2007-02-12 2009-10-19 안트로제네시스 코포레이션 부착성 태반 줄기세포에서 유래한 간세포와 연골세포 및 ｃｄ34＋, ｃｄ45－ 태반 줄기세포가 농축된 세포군
KR101372384B1 (ko) * 2009-04-27 2014-03-12 취토넷 게엠베하 운트 코. 카게 캡슐화된 간세포 조성물
KR20160121780A (ko) * 2015-04-09 2016-10-20 한양대학교 산학협력단 세포와 세포간의 결합 증진을 위한 고분자 지지체 및 이를 이용한 세포 배양 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020066507A (ko) * 2001-02-12 2002-08-19 이종원 항생제를 이용한 인공간 시스템
KR20090109127A (ko) * 2007-02-12 2009-10-19 안트로제네시스 코포레이션 부착성 태반 줄기세포에서 유래한 간세포와 연골세포 및 ｃｄ34＋, ｃｄ45－ 태반 줄기세포가 농축된 세포군
KR101372384B1 (ko) * 2009-04-27 2014-03-12 취토넷 게엠베하 운트 코. 카게 캡슐화된 간세포 조성물
KR20160121780A (ko) * 2015-04-09 2016-10-20 한양대학교 산학협력단 세포와 세포간의 결합 증진을 위한 고분자 지지체 및 이를 이용한 세포 배양 방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHOE, G.: "Biomimetic three dimensional culture of human mesenchymal stem cells for highly efficient and scalable cell expansion", KOREAN SOCIETY FOR BIOMATERIALS FALL CONFERENCE AND GENERAL MEETING, vol. 21, no. 3, 19 October 2017 (2017-10-19) *
MOON, JONG SIK: "Microencapsulation and Metabolic Feature of Hepatocyte Using Alginate and Acrylic Polymer", THESIS MASTER GRADUATE SCHOOL, KYUNGPOOK NATIONAL UNIVERSITY: DEPARTMENT OF POLYMER SCIENCE, 1 July 2007 (2007-07-01), pages 1 - 75 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108949524A (zh) * 2018-07-26 2018-12-07 上海大学 用于体外细胞三维微组织形成的笼结构微孔培养皿及其制备方法
CN108949524B (zh) * 2018-07-26 2021-10-08 上海大学 用于体外细胞三维微组织形成的笼结构微孔培养皿及其制备方法
CN111375361A (zh) * 2020-03-17 2020-07-07 北京唐颐惠康生物医学技术有限公司 一种用于大规模干细胞培养的纳米海藻糖3d微胶囊
CN113699094A (zh) * 2021-08-26 2021-11-26 深圳钧兴生物科技有限公司 一种无血清培养细胞的方法

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