WO2018056659A1 - Scented vinegar composition and preparation method therefor - Google Patents

Scented vinegar composition and preparation method therefor Download PDF

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Publication number
WO2018056659A1
WO2018056659A1 PCT/KR2017/010193 KR2017010193W WO2018056659A1 WO 2018056659 A1 WO2018056659 A1 WO 2018056659A1 KR 2017010193 W KR2017010193 W KR 2017010193W WO 2018056659 A1 WO2018056659 A1 WO 2018056659A1
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Prior art keywords
vinegar
extract
fragrant
producing
fragrance
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PCT/KR2017/010193
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French (fr)
Korean (ko)
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신만섭
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주식회사 엘지생활건강
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00Disinfection, sterilisation or deodorisation of air
    • A61L9/01Deodorant compositions
    • A61L9/013Deodorant compositions containing animal or plant extracts, or vegetable material
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/0005Other compounding ingredients characterised by their effect
    • C11D3/001Softening compositions
    • C11D3/0015Softening compositions liquid
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/0005Other compounding ingredients characterised by their effect
    • C11D3/0068Deodorant compositions
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/382Vegetable products, e.g. soya meal, wood flour, sawdust
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12JVINEGAR; PREPARATION OR PURIFICATION THEREOF
    • C12J1/00Vinegar; Preparation or purification thereof

Definitions

  • the present invention relates to a fragrant vinegar composition and a method for producing the same.
  • Vinegar is a naturally derived food raw material manufactured by fermenting grains, fruits or herbs. These vinegars vary in the degree of fermentation depending on the raw materials used, and there are many manufacturing processes that can utilize the characteristics of the raw materials. In addition, vinegar is a weak acid containing acetic acid, and is used as a detergent and deodorant, and in some cases it is used as a substitute for fabric softener and hair rinse. Despite its use, vinegar has many difficulties in storage or use due to its strong acetic acid odor.
  • Patent Document 1 In order to remove the odor of such vinegar, a method of increasing the off-flavor by inoculating parasitic acetic acid fermentation bacteria or by performing alcohol fermentation has been proposed (Patent Document 1). However, this method is applicable to the raw materials in which carbohydrates exist, and there are limitations on the use of various raw materials.
  • Patent Document 1 Korea Patent Publication 10-2004-0032513
  • the present invention provides a method for producing a fragrant vinegar composition comprising the step of preparing a fermented vinegar by fermenting the mixture of grain extract and the flavor component concentrate.
  • the present invention comprises the step of preparing a fermented vinegar by fermenting the mixture of grain extract and the concentrated component concentrate;
  • It provides a method for producing a fragrant vinegar composition comprising the step of mixing the fragrance component extract with the fermented vinegar.
  • the present invention provides a fragrant vinegar composition prepared by the above-described manufacturing method.
  • the fragrant vinegar composition according to the present invention has no sense of discomfort in use because acetic acid odor is not detected, and has an excellent odor.
  • various flavors can be realized through a change in selection of raw materials (fragrance components).
  • the vinegar prepared above can be used as a cleaning agent by itself, and can be used as a rinse, fabric softener and deodorant.
  • 1 is a schematic diagram showing a method for producing fragrant vinegar according to an example of the present invention.
  • the present invention relates to a method for producing a fragrant vinegar composition
  • a method for producing a fragrant vinegar composition comprising the step of preparing a fermented vinegar by fermenting the mixture of grain extract and the flavor component concentrate.
  • grain component of the grain extract in the present invention can be used without limitation grains used in the manufacture of vinegar in the art, for example, rice, barley, wheat or a mixture thereof may be used.
  • the grain extract may be hot water, ethanol or reduced pressure extract of the grain, specifically, the hot water extract of grain.
  • the grain extract may be an extract extracted after immersing the grain in the extraction solvent of 60 to 90 °C using purified water as the extraction solvent.
  • the extract can be used after filtration using a separate filtration device.
  • the fragrance concentrate (hereinafter, referred to as a fragrance concentrate) may be used to reduce acetic acid odor generated during fermentation of grains and the like, and to give a good flavor to vinegar.
  • the fragrance ingredients are cinnamon, aronia, hibiscus, gardenia, white rice, lemon balm, chamomile, peppermint, spearmint, sage, velgam, basil, thyme, oregano, acacia, lily, lotus, jasmine, rose, lavender, Chrysanthemum, lilac, apricot, freesia, tulip, eucalyptus, rosemary, lilac, magnolia, applemint, tea tree, hyacinth, cherry blossom, lemon verbena, camellia, chanel, peach blossom, blueberry, raspberry or mixtures thereof have.
  • the fragrance concentrate may be cold concentrate, low temperature, hot water, reduced pressure or concentrate of ethanol extract of the fragrance component.
  • the fragrance concentrate is 60 to 90% or 60 to 70% (v / v) using ethanol as the extraction solvent, the fragrance components at a temperature of 15 to 30 °C or 20 to 25 °C and 800 to 1000 mmHg Or extracted at a pressure of 850-950 mmHg.
  • the extracted fragrance components can be concentrated to 10 to 20% (v / v) the content of ethanol using a reduced pressure, concentration or distillation apparatus used in the art.
  • the concentrate can be used after filtration using a separate filtration device.
  • Fermentation vinegar in the present invention may be prepared through the step of fermentation, after mixing the grain extract and the fragrance concentrate.
  • the intrinsic flavor of the fragrance concentrate can more effectively reduce the odor derived from the strong acetic odor and grain extract appearing in the vinegar fermentation process.
  • Vinegar using the grain extract is a unique smell of grain occurs, which is mixed with acetic acid odor there is a fear of causing an unpleasant smell.
  • acetic acid odor may be partially weakened, but there is a problem that a characteristic unpleasant odor still remains. Therefore, in the present invention, the odor including acetic acid odor is greatly reduced by fermentation after mixing the grain extract and the fragrance concentrate, the effect can be long lasting.
  • the content of the fragrance concentrate may be 20 to 100 parts by weight, 30 to 70 parts by weight or 40 to 60 parts by weight based on 100 parts by weight of the grain extract. It is possible to more effectively reduce the acetic acid odor appearing in the fermentation process in the above content range. If the content is less than 20 parts by weight there is a concern that the problem of acetic acid odor removal.
  • Fermentation in the present invention may be carried out through the step of acetic acid fermentation, after the mixture ethanol fermentation.
  • Ethanol fermentation refers to fermentation obtained by decomposing sugar such as fructose into carbon dioxide and ethanol by yeast. Such ethanol fermentation can be carried out through the usual methods in the art.
  • acetic acid fermentation is a fermentation phenomenon in which acetic acid (acetic acid) is formed in ethyl alcohol by oxidation reaction by acetic acid bacteria, and is fermentation used for the production of vinegar.
  • Such acetic acid fermentation may be carried out by culturing the product produced by the aforementioned ethanol fermentation into a medium containing acetic acid bacteria.
  • the acetic acid fermentation may be carried out using a general method in the art, and through the fermentation it can be prepared fermented vinegar.
  • the present invention may further comprise the step of mixing the scent component extract (hereinafter referred to as the scent extract) to the fermented vinegar.
  • the mixed extract can provide a fragrant vinegar composition is sensed as a fragrance to the user when using the vinegar.
  • the fragrance component in the fragrance extract may be the same kind as the fragrance component of the above-mentioned fragrance concentrate, for example, cinnamon, aronia, hibiscus, gardenia, white rice, lemon balm, chamomile, peppermint, spearmint, sage, velgam , Basil, thyme, oregano, acacia, lily, lotus, jasmine, rose, lavender, chrysanthemum, lilac, apricot, freesia, tulip, eucalyptus, rosemary, lilac, magnolia, applemint, tea tree, hyacinth, cherry blossom , Lemon verbena, camellia, chanel, peach blossom, blueberry, raspberry or mixtures thereof may be used.
  • two or more of the above components may be mixed and used according to the type of aroma desired.
  • the fragrance extract can be prepared by extracting the fragrance components cold, low temperature, hot water, reduced pressure or ethanol.
  • the cold needle extraction, low temperature extraction, hot water extraction, reduced pressure extraction or ethanol extraction may be performed according to the general method in the art.
  • the content of the fragrance extract may be 0.5 to 20 parts by weight, 1 to 15 parts by weight or 5 to 10 parts by weight based on 100 parts by weight of the fermented vinegar. It can reduce the acetic acid odor in the above content range, it can give a scent to the vinegar.
  • the present invention may further comprise the step of aging the mixture of the fermentation vinegar and the flavor component extract.
  • the aging may be performed at 5 to 20 ° C. or 10 to 15 ° C. for 3 to 14 days or 6 to 10 days.
  • the present invention comprises the steps of fermenting a mixture of grain extract and aroma component concentrate to prepare a fermented vinegar
  • It relates to a method for producing a fragrant vinegar composition
  • a method for producing a fragrant vinegar composition comprising the step of mixing the fragrance component extract with the fermented vinegar.
  • the present invention it is possible to more effectively reduce the odor of acetic acid produced during the fermentation process of vinegar by using the scent component extract and scent component extract, it can give a good flavor to the vinegar produced.
  • the present invention also relates to a fragrant vinegar composition produced by the above-mentioned manufacturing method.
  • the vinegar composition prepared according to the present invention can be used as a cleaning agent by itself, and can be used as a rinse, fabric softener, and deodorant.
  • Cinnamon (Preparation Example 1A), Aronia (Preparation Example 1B), Hibiscus (Preparation Example 1C), Gardenia (Preparation Example 1D) or White rice (Preparation Example 1E) was used as a dried vegetable raw material.
  • acetic acid JTBaker, Acetic acid, glacial 99.7%
  • 6% g / v
  • Comparative Preparation Example 1A a mixture obtained by mixing 6% (g / v) of acetic acid in purified water was used as Comparative Preparation Example 1A, Otogi's Brown Rice Vinegar as Comparative Preparation Example 1B, and Brown Rice Vinegar with Clean Yarn as Comparative Preparation Example 1C. It was.
  • Acetobactor aceti was stored in GYC slant (glucose 50 g / L, yeast extract 10 g / L, CaCO 3 30 g / L, agar 15 g / L, pH 6.3) and passaged every month to incubate at 28 ° C.
  • GYC slant glucose 50 g / L, yeast extract 10 g / L, CaCO 3 30 g / L, agar 15 g / L, pH 6.3
  • Culture medium for inoculation was 1.1 g / L glucose, 0.2 g / L yeast extract, 10 g / L peptone, (NH 4 ) 2 HPO 4 1.4 g / L, KH 2 PO 4 0.14 g / L, MgSO 4 (7H 2 O) 0.02 g / L, acetic acid 21 g / L, ethanol (95%) 42 g / L was used to prepare the composition, volatile ethanol was 0.22 PVDF filter after media sterilization It was added by filtration.
  • Strain culture for inoculation was put 50 ml of inoculation culture medium in a 300 ml baffle flask, sterilized and inoculated with bacteria (acetic acid) in a loop and incubated for 48 hours at 200 rpm, 30 °C in a shaker.
  • bacteria acetic acid
  • Purified water was added to the fermented vinegar prepared in Preparation Example 3 to adjust the content of the final acetic acid to about 6% and the odor was evaluated.
  • the odor measurement was performed by the same method as the odor measurement of Experimental Example 1 described above.
  • Table 3 shows the results of comparing the odor of the fermented vinegar.
  • Dried vegetable raw material that is, cinnamon, aronia, hibiscus, gardenia or white rice was used as a fragrance component.
  • the amount of the extraction solvent was 10 ⁇ 20 ml per 1 g of the dry weight of the crushed raw material and extraction conditions were extracted for 3 hours, 150 rpm, -500 mmHg stirring / decompression at the same temperature conditions after 10 hours immersion at 10 °C.
  • the extract was filtered through a filter paper (Advantec. Toyo Roshi Kaisha, Japan) to prepare a fragrance extract.
  • the fragrance extract prepared in (1) was added to the fermented vinegar.
  • the fragrance extract was added to the fermented vinegar in a content ratio of 10% (w / v).
  • the mixed solution of (2) was aged at 10 ° C. for 7 days.
  • the manufacturing method of the manufacturing method of the scented vinegar may be manufactured according to the method of FIG.
  • fragrance components of the fragrance concentrate and fragrance extract used in the scented vinegar are listed in Table 4 below.
  • Comparative Preparation 4A a mixture of acetic acid 6% (g / v) was used in purified water, and in Comparative Preparation 4B, fermentation was made after mixing 6% (g / v) of acetic acid and 10% of ethanol. It was used by fermentation, fermented after mixing grain extract and ethanol 10% in Comparative Preparation Example 4C, and mixed with the fragrance extract (garia extract) and then used by fermentation.
  • the odor measurement was performed by the same method as the odor measurement of Experimental Example 1 described above.
  • the scented vinegar composition according to the present invention can be used as a cleaning agent by itself, and can be used as a rinse, a fabric softener, and a deodorant.

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Abstract

The present invention relates to scented vinegar and a preparation method therefor. A scented vinegar composition, according to the present invention, does not cause unpleasantness when using same since a smell of acetic acid is not sensed therefrom, and exhibits an excellent scent. In addition, various scents may be materialized by altering scented components. In addition, the vinegar composition, according to the present invention, may be in itself used as a cleaning agent and may be used for the purpose of a hair conditioner, a fabric softener and a deodorizer.

Description

향기 나는 식초 조성물 및 그 제조방법Aromatic vinegar composition and preparation method thereof
본 발명은 향기 나는 식초 조성물 및 그의 제조방법에 관한 것이다.The present invention relates to a fragrant vinegar composition and a method for producing the same.
근래 국내외 생활용품 시장에서 천연 유래 제품에 대한 소비가 급증하면서 식품원료의 사용이 증가하고 있다. 특히 베이킹소다, 식초 및 구연산 등의 세정제 대용 식품원료가 다양하게 활용되고 있다. 그러나, 원료 그대로를 사용하기에는 특유의 냄새나 계량, 이송 등의 사용성이 좋지 못한 단점을 가진다. 식초의 경우, 식품공전 및 식약처 규정 상 아세트산을 4% 이상 함유하여야 하므로, 특유의 취의 근본적인 제거는 불가능한 실정이다.In recent years, the consumption of food products is increasing as the consumption of natural-derived products has soared in the domestic and foreign household goods market. In particular, various food substitutes for cleaning agents such as baking soda, vinegar, and citric acid have been utilized. However, in order to use the raw material as it is, there is a disadvantage that the usability, such as the peculiar smell, metering, transporting is not good. In the case of vinegar, it is necessary to contain at least 4% of acetic acid according to the regulations of the Food Code and the Ministry of Food and Drug Administration.
식초는 곡물, 과실 또는 허브 등을 발효하여 제조하는 천연유래 식품원료이다. 이러한 식초는 사용 원물에 따라 발효되는 정도가 다르며, 원물의 특성을 살릴 수 있는 많은 제조공정들이 존재한다. 또한, 식초는 아세트산을 포함하는 약산으로, 세정제 및 탈취제 등으로 사용되며 일부에서는 섬유유연제 및 헤어 린스의 대용으로 사용되기도 한다. 이러한 용도가 있음에도 불구하고, 식초는 아세트산 냄새가 강한 특성 때문에 보관 또는 사용 과정에서 어려움이 많다.Vinegar is a naturally derived food raw material manufactured by fermenting grains, fruits or herbs. These vinegars vary in the degree of fermentation depending on the raw materials used, and there are many manufacturing processes that can utilize the characteristics of the raw materials. In addition, vinegar is a weak acid containing acetic acid, and is used as a detergent and deodorant, and in some cases it is used as a substitute for fabric softener and hair rinse. Despite its use, vinegar has many difficulties in storage or use due to its strong acetic acid odor.
이러한 식초의 냄새를 제거하기 위하여, 기생성된 초산발효균을 접종하여 증자 발효를 수행하거나, 알코올 발효를 수행하여 이취발생을 낮추는 방법이 제안되었다(특허문헌 1). 그러나 이러한 방법은 탄수화물이 존재하는 원물에 응용 가능한 방법으로서, 다양한 원물의 사용에 한계가 있다.In order to remove the odor of such vinegar, a method of increasing the off-flavor by inoculating parasitic acetic acid fermentation bacteria or by performing alcohol fermentation has been proposed (Patent Document 1). However, this method is applicable to the raw materials in which carbohydrates exist, and there are limitations on the use of various raw materials.
특허문헌 1. 한국 공개특허 10-2004-0032513Patent Document 1. Korea Patent Publication 10-2004-0032513
본 발명은 식초의 발효과정에서 생성되는 아세트산 취가 제거된 향기 나는 식초 조성물 및 그 제조방법을 제공하는 것을 목적으로 한다.It is an object of the present invention to provide a fragrant vinegar composition from which acetic acid odor generated in the fermentation process of vinegar is removed and a method for producing the same.
본 발명에서는 곡물 추출물 및 향기성분 농축액의 혼합물을 발효하여 발효식초를 제조하는 단계를 포함하는 향기 나는 식초 조성물의 제조 방법을 제공한다. The present invention provides a method for producing a fragrant vinegar composition comprising the step of preparing a fermented vinegar by fermenting the mixture of grain extract and the flavor component concentrate.
또한, 본 발명에서는 곡물 추출물 및 향기성분 농축액의 혼합물을 발효하여 발효식초를 제조하는 단계; 및 In addition, the present invention comprises the step of preparing a fermented vinegar by fermenting the mixture of grain extract and the concentrated component concentrate; And
상기 발효식초에 향기성분 추출액을 혼합하는 단계를 포함하는 향기 나는 식초 조성물의 제조 방법을 제공한다.It provides a method for producing a fragrant vinegar composition comprising the step of mixing the fragrance component extract with the fermented vinegar.
또한, 본 발명에서는 전술한 제조 방법에 의해 제조된 향기 나는 식초 조성물을 제공한다.In addition, the present invention provides a fragrant vinegar composition prepared by the above-described manufacturing method.
본 발명에 따른 향기 나는 식초 조성물은 아세트산 취가 감지되지 않으므로 사용에 불쾌감이 없으며, 우수한 향취를 가진다. 또한, 원물(향기성분) 선택의 변화를 통해 다양한 향취를 구현할 수 있다. The fragrant vinegar composition according to the present invention has no sense of discomfort in use because acetic acid odor is not detected, and has an excellent odor. In addition, various flavors can be realized through a change in selection of raw materials (fragrance components).
또한, 본 발명에서는 식초의 생산 수율을 증대시킬 수 있으며, 상기 제조되는 식초는 그 자체로 세정제로 사용가능하며, 린스, 섬유유연제 및 탈취제 등의 용도로 사용 가능하다.In addition, in the present invention, it is possible to increase the production yield of vinegar, and the vinegar prepared above can be used as a cleaning agent by itself, and can be used as a rinse, fabric softener and deodorant.
도 1은 본 발명의 일례에 따른 향기나는 식초의 제조 방법을 나타내는 모식도이다.1 is a schematic diagram showing a method for producing fragrant vinegar according to an example of the present invention.
본 발명은 곡물 추출물 및 향기성분 농축액의 혼합물을 발효하여 발효식초를 제조하는 단계를 포함하는 향기 나는 식초 조성물의 제조 방법에 관한 것이다. The present invention relates to a method for producing a fragrant vinegar composition comprising the step of preparing a fermented vinegar by fermenting the mixture of grain extract and the flavor component concentrate.
이하, 본 발명에 따른 향기 나는 식초 조성물을 보다 상세하게 설명한다. Hereinafter, the fragrant vinegar composition according to the present invention will be described in more detail.
본 발명에서 곡물 추출물의 곡물 성분으로는 당업계에서 식초의 제조에 사용되는 곡물을 제한없이 사용할 수 있으며, 예를 들어 쌀, 보리, 밀 또는 이들의 혼합물을 사용할 수 있다. As the grain component of the grain extract in the present invention can be used without limitation grains used in the manufacture of vinegar in the art, for example, rice, barley, wheat or a mixture thereof may be used.
상기 곡물 추출물은 곡물의 열수, 에탄올 또는 감압 추출물일 수 있으며, 구체적으로 곡물의 열수 추출물일수 있다. 일 구체예에서, 곡물 추출물은 정제수를 추출용매로 사용하여 곡물을 60 내지 90℃의 추출용매에 침지한 후 추출한 추출물일 수 있다. 상기 추출물은 별도의 여과장치를 사용하여 여과한 후 사용할 수 있다. The grain extract may be hot water, ethanol or reduced pressure extract of the grain, specifically, the hot water extract of grain. In one embodiment, the grain extract may be an extract extracted after immersing the grain in the extraction solvent of 60 to 90 ℃ using purified water as the extraction solvent. The extract can be used after filtration using a separate filtration device.
본 발명에서 향기성분 농축액(이하, 향기 농축액이라 할 수 있다.)은 곡물 등의 발효시 생성되는 아세트산 취를 감소시키고, 식초에 좋은 향을 부여하기 위하여 사용할 수 있다. In the present invention, the fragrance concentrate (hereinafter, referred to as a fragrance concentrate) may be used to reduce acetic acid odor generated during fermentation of grains and the like, and to give a good flavor to vinegar.
상기 향기성분은 계피, 아로니아, 히비스커스, 치자, 백미, 레몬밤, 캐모마일, 페퍼민트, 스피아민트, 세이지, 벨가못, 바질, 타임, 오레가노, 아카시아꽃, 백합, 연꽃, 자스민, 장미, 라벤터, 국화, 라일락, 살구, 후리지아, 튤립, 유칼립튜스, 로즈마리, 라일락, 목련, 애플민트, 티트리, 히아신스, 벚꽃, 레몬버베나, 동백, 훤넬, 복숭아꽃, 블루베리, 라즈베리 또는 이들의 혼합물일 수 있다. The fragrance ingredients are cinnamon, aronia, hibiscus, gardenia, white rice, lemon balm, chamomile, peppermint, spearmint, sage, velgam, basil, thyme, oregano, acacia, lily, lotus, jasmine, rose, lavender, Chrysanthemum, lilac, apricot, freesia, tulip, eucalyptus, rosemary, lilac, magnolia, applemint, tea tree, hyacinth, cherry blossom, lemon verbena, camellia, chanel, peach blossom, blueberry, raspberry or mixtures thereof have.
또한, 상기 향기 농축액은 향기성분의 냉침, 저온, 열수, 감압 또는 에탄올 추출물의 농축물일 수 있다. 일 구체예에서, 상기 향기 농축액은 60 내지 90% 또는 60 내지 70%(v/v) 에탄올을 추출용매로 사용하여, 향기성분을 15 내지 30℃ 또는 20 내지 25℃의 온도 및 800 내지 1000 mmHg 또는 850 내지 950 mmHg의 압력에서 추출하여 제조할 수 있다. 또한, 일 구체예에서 상기 추출된 향기성분은 당업계에서 사용되는 감압, 농축 또는 증류 장치를 사용하여 에탄올의 함량을 10 내지 20 %(v/v)까지 농축할 수 있다. 상기 농축물은 별도의 여과장치를 사용하여 여과한 후 사용할 수 있다.In addition, the fragrance concentrate may be cold concentrate, low temperature, hot water, reduced pressure or concentrate of ethanol extract of the fragrance component. In one embodiment, the fragrance concentrate is 60 to 90% or 60 to 70% (v / v) using ethanol as the extraction solvent, the fragrance components at a temperature of 15 to 30 ℃ or 20 to 25 ℃ and 800 to 1000 mmHg Or extracted at a pressure of 850-950 mmHg. In addition, in one embodiment the extracted fragrance components can be concentrated to 10 to 20% (v / v) the content of ethanol using a reduced pressure, concentration or distillation apparatus used in the art. The concentrate can be used after filtration using a separate filtration device.
본 발명에서 발효식초는 상기 곡물 추출물 및 향기 농축액을 혼합한 뒤, 발효하는 단계를 통해 제조할 수 있다. 본 발명에서는 곡물 추출물 및 향기 농축액을 각각 제조 및 혼합한 뒤 발효함으로서, 향기 농축액의 고유 향취가 식초 발효과정에서 나타나는 강한 아세트산 취와 곡물 추출물에서 나오는 이취를 보다 효과적으로 감소시킬 수 있다.Fermentation vinegar in the present invention may be prepared through the step of fermentation, after mixing the grain extract and the fragrance concentrate. In the present invention, by producing and mixing the grain extract and the fragrance concentrate, respectively, and fermentation, the intrinsic flavor of the fragrance concentrate can more effectively reduce the odor derived from the strong acetic odor and grain extract appearing in the vinegar fermentation process.
곡물 추출물을 이용한 식초는 곡물 특유의 이취가 발생하고, 이는 아세트산 취와 혼합되어 불쾌한 냄새를 발생시킬 우려가 있다. 곡물 추출물에 단순히 향기 성분을 첨가하는 경우에는 아세트산 취는 일부 약해질 수 있으나, 특유의 불쾌한 냄새는 여전히 남게 되는 문제가 있다. 따라서, 본 발명에서는 곡물 추출물과 향기 농축액을 혼합한 뒤 발효함으로써 아세트산 취를 포함한 이취가 크게 줄어들고, 이와 같은 효과가 오래 지속될 수 있다.Vinegar using the grain extract is a unique smell of grain occurs, which is mixed with acetic acid odor there is a fear of causing an unpleasant smell. In the case of simply adding the fragrance component to the grain extract, acetic acid odor may be partially weakened, but there is a problem that a characteristic unpleasant odor still remains. Therefore, in the present invention, the odor including acetic acid odor is greatly reduced by fermentation after mixing the grain extract and the fragrance concentrate, the effect can be long lasting.
상기 향기 농축액의 함량은 곡물 추출물 100 중량부에 대하여 20 내지 100 중량부, 30 내지 70 중량부 또는 40 내지 60 중량부일 수 있다. 상기 함량 범위에서 발효 과정에서 나타나는 아세트산 취를 보다 효과적으로 감소시킬 수 있다. 상기 함량이 20 중량부 미만일 경우 아세트산 취 제거에 문제가 발생할 우려가 있다. The content of the fragrance concentrate may be 20 to 100 parts by weight, 30 to 70 parts by weight or 40 to 60 parts by weight based on 100 parts by weight of the grain extract. It is possible to more effectively reduce the acetic acid odor appearing in the fermentation process in the above content range. If the content is less than 20 parts by weight there is a concern that the problem of acetic acid odor removal.
본 발명에서 발효는 상기 혼합물을 에탄올 발효한 뒤, 초산 발효하는 단계를 통해 수행할 수 있다. Fermentation in the present invention may be carried out through the step of acetic acid fermentation, after the mixture ethanol fermentation.
에탄올 발효는 과당과 같은 당분이 효모에 의하여 이산화 탄소와 에탄올로 분해하여 이루어지는 발효를 의미한다. 이러한 에탄올 발효는 당업계의 일반적인 방법을 통해 수행할 수 있다.Ethanol fermentation refers to fermentation obtained by decomposing sugar such as fructose into carbon dioxide and ethanol by yeast. Such ethanol fermentation can be carried out through the usual methods in the art.
또한, 초산 발효는 초산균에 의하여 산화반응을 하여 에틸 알코올에서 초산(아세트산)이 생기는 발효 현상으로, 식초의 제조에 이용되는 발효이다. In addition, acetic acid fermentation is a fermentation phenomenon in which acetic acid (acetic acid) is formed in ethyl alcohol by oxidation reaction by acetic acid bacteria, and is fermentation used for the production of vinegar.
이러한 초산 발효는 전술한 에탄올 발효에 의해 생성된 생성물을 초산균을 포함하는 배지에 넣고 배양하여 수행할 수 있다. 상기 초산 발효는 당업계의 일반적인 방법을 사용하여 수행할 수 있으며, 상기 발효를 통해 발효식초를 제조할 수 있다. Such acetic acid fermentation may be carried out by culturing the product produced by the aforementioned ethanol fermentation into a medium containing acetic acid bacteria. The acetic acid fermentation may be carried out using a general method in the art, and through the fermentation it can be prepared fermented vinegar.
또한, 본 발명에서는 발효식초에 향기성분 추출액(이하, 향기 추출액이라 할 수 있다)을 혼합하는 단계를 추가로 포함할 수 있다. In addition, the present invention may further comprise the step of mixing the scent component extract (hereinafter referred to as the scent extract) to the fermented vinegar.
본 발명에서는 발효식초에 향기 추출액을 추가로 혼합함으로써, 아세트산 취를 2차적으로 감소시킬 수 있으며, 상기 혼합된 추출액은 식초의 사용시 사용자에게 향료로 감지되어 향기 나는 식초 조성물을 제공할 수 있다. In the present invention, by further mixing the fragrance extract with fermented vinegar, acetic acid odor can be reduced secondly, the mixed extract can provide a fragrant vinegar composition is sensed as a fragrance to the user when using the vinegar.
상기 향기 추출액에서 향기성분은 전술한 향기 농축액의 향기성분과 같은 종류를 사용할 수 있으며, 예를 들어 계피, 아로니아, 히비스커스, 치자, 백미, 레몬밤, 캐모마일, 페퍼민트, 스피아민트, 세이지, 벨가못, 바질, 타임, 오레가노, 아카시아꽃, 백합, 연꽃, 자스민, 장미, 라벤터, 국화, 라일락, 살구, 후리지아, 튤립, 유칼립튜스, 로즈마리, 라일락, 목련, 애플민트, 티트리, 히아신스, 벚꽃, 레몬버베나, 동백, 훤넬, 복숭아꽃, 블루베리, 라즈베리 또는 이들의 혼합물을 사용할 수 있다. 본 발명에서는 원하는 향의 종류에 따라 상기 성분을 둘 이상 혼합하여 사용할 수 있다. The fragrance component in the fragrance extract may be the same kind as the fragrance component of the above-mentioned fragrance concentrate, for example, cinnamon, aronia, hibiscus, gardenia, white rice, lemon balm, chamomile, peppermint, spearmint, sage, velgam , Basil, thyme, oregano, acacia, lily, lotus, jasmine, rose, lavender, chrysanthemum, lilac, apricot, freesia, tulip, eucalyptus, rosemary, lilac, magnolia, applemint, tea tree, hyacinth, cherry blossom , Lemon verbena, camellia, chanel, peach blossom, blueberry, raspberry or mixtures thereof may be used. In the present invention, two or more of the above components may be mixed and used according to the type of aroma desired.
상기 향기 추출액은 향기성분을 냉침, 저온, 열수, 감압 또는 에탄올 추출하여 제조할 수 있다. 상기 냉침 추출, 저온 추출, 열수 추출, 감압 추출 또는 에탄올 추출은 당업계의 일반적인 방법에 따라 수행할 수 있다.The fragrance extract can be prepared by extracting the fragrance components cold, low temperature, hot water, reduced pressure or ethanol. The cold needle extraction, low temperature extraction, hot water extraction, reduced pressure extraction or ethanol extraction may be performed according to the general method in the art.
또한, 향기 추출액의 함량은 발효식초 100 중량부에 대하여 0.5 내지 20 중량부, 1 내지 15 중량부 또는 5 내지 10 중량부일 수 있다. 상기 함량 범위에서 아세트산 취를 감소시킬 수 있으며, 식초에 향기를 부여할 수 있다. In addition, the content of the fragrance extract may be 0.5 to 20 parts by weight, 1 to 15 parts by weight or 5 to 10 parts by weight based on 100 parts by weight of the fermented vinegar. It can reduce the acetic acid odor in the above content range, it can give a scent to the vinegar.
또한, 본 발명에서는 발효식초 및 향기성분 추출액의 혼합물을 숙성하는 단계를 추가로 포함할 수 있다. 상기 숙성은 5 내지 20℃ 또는 10 내지 15℃에서 3 내지 14일 또는 6 내지 10일 동한 수행할 수 있다. In addition, the present invention may further comprise the step of aging the mixture of the fermentation vinegar and the flavor component extract. The aging may be performed at 5 to 20 ° C. or 10 to 15 ° C. for 3 to 14 days or 6 to 10 days.
또한, 본 발명은 곡물 추출물 및 향기성분 농축액의 혼합물을 발효하여 발효식초를 제조하는 단계; 및 In addition, the present invention comprises the steps of fermenting a mixture of grain extract and aroma component concentrate to prepare a fermented vinegar; And
상기 발효식초에 향기성분 추출액을 혼합하는 단계를 포함하는 향기 나는 식초 조성물의 제조 방법에 관한 것이다. It relates to a method for producing a fragrant vinegar composition comprising the step of mixing the fragrance component extract with the fermented vinegar.
본 발명에서는 향기성분 농축액 및 향기성분 추출액을 사용하여 식초의 발효과정에서 생산되는 아세트산의 취를 보다 효과적으로 감소시킬 수 있으며, 제조되는 식초에 좋은 향을 부여할 수 있다. In the present invention, it is possible to more effectively reduce the odor of acetic acid produced during the fermentation process of vinegar by using the scent component extract and scent component extract, it can give a good flavor to the vinegar produced.
또한, 본 발명은 전술한 제조 방법에 의해 제조된 향기 나는 식초 조성물에 관한 것이다. The present invention also relates to a fragrant vinegar composition produced by the above-mentioned manufacturing method.
본 발명에 의해 제조되는 식초 조성물은 그 자체로 세정제로 사용가능하며, 린스, 섬유유연제 및 탈취제 등의 용도로 사용 가능하다.The vinegar composition prepared according to the present invention can be used as a cleaning agent by itself, and can be used as a rinse, fabric softener, and deodorant.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.
실시예Example
제조예Production Example 1. 향기 농축액 제조 1. Fragrance Concentrate Preparation
건조된 식물성 원재료, 즉 향기성분으로 계피(제조예 1A), 아로니아(제조예 1B), 히비스커스(제조예 1C), 치자(제조예 1D) 또는 백미(제조예 1E)를 사용하였다. Cinnamon (Preparation Example 1A), Aronia (Preparation Example 1B), Hibiscus (Preparation Example 1C), Gardenia (Preparation Example 1D) or White rice (Preparation Example 1E) was used as a dried vegetable raw material.
상기 원재료를 믹서로 파쇄한 후, 추출용매로서 70% (v/v) 에탄올 (대한주정라이프, 95% 발효주정)을 첨가하였다. 이때 추출용매의 양은 파쇄된 원재료의 건조중량 1 g 당 10~20 ml로 하였으며 추출조건은 실온에서 2 시간 침지 후, 3 시간, 150 rpm 및 900 mmHg 조건에서 추출하였다. 추출물은 filter paper(Advantec. Toyo Roshi Kaisha, Japan)를 이용하여 여과한 후 냉각 콘덴서가 장착된 증류장치로 에탄올을 10~20% (v/v)까지 감압/농축하여 향기 농축액을 제조하였다.After crushing the raw material with a mixer, 70% (v / v) ethanol (Korean alcohol, 95% fermented alcohol) was added as an extraction solvent. At this time, the amount of the extraction solvent was 10 ~ 20 ml per 1 g of the dry weight of the crushed raw material and the extraction conditions were extracted at 150 rpm and 900 mmHg conditions for 3 hours after immersion at room temperature for 2 hours. The extract was filtered using a filter paper (Advantec. Toyo Roshi Kaisha, Japan), and then concentrated / reduced to 10-20% (v / v) of ethanol using a distillation apparatus equipped with a cooling condenser to prepare a fragrance concentrate.
실험예Experimental Example 1. 식초발효용 향기 농축액 선별 1. Selection of fragrance concentrate for vinegar fermentation
(1) 아세트산 취 측정 방법(1) acetic acid odor measurement method
아세트산 취가 감소한 발효식초를 제조하기 위하여, 제조예 1에서 제조된 향기 농축액에 아세트산(J.T.Baker, Acetic acid, glacial 99.7%)을 6% (g/v)로 혼합하여 아세트산의 향취 감소를 확인하였다. In order to prepare the fermented vinegar with reduced acetic acid odor, acetic acid (JTBaker, Acetic acid, glacial 99.7%) was mixed with 6% (g / v) in the fragrance concentrate prepared in Preparation Example 1 to confirm the decrease in the odor of acetic acid. .
이때, 비교-제조예 1A로 정제수에 아세트산을 6% (g/v) 혼합한 혼합물을 사용하였으며, 비교-제조예 1B로 오뚜기사의 현미식초를, 비교-제조예 1C로 청정원사의 현미식초를 사용하였다. In this case, a mixture obtained by mixing 6% (g / v) of acetic acid in purified water was used as Comparative Preparation Example 1A, Otogi's Brown Rice Vinegar as Comparative Preparation Example 1B, and Brown Rice Vinegar with Clean Yarn as Comparative Preparation Example 1C. It was.
상기 아세트산이 혼합된 향기 농축액에 대하여 20 내지 45세의 남자 7명 및 여자 7명에게 아세트산의 혼합을 공지하지 않고 냄새를 맡게 하였다. 아세트산 취의 강도에 따른 판정 척도는 하기 표 1과 같이 5점 평점법으로 실시하였다. 향취 테스트는 2시간 간격으로 진행하였으며 1일 2개이상의 시료를 적용하지 않았다.Seven men and seven women aged 20 to 45 years were allowed to smell the mixture of acetic acid without knowing the acetic acid mixture. The determination scale according to the strength of acetic acid odor was performed by the 5-point scoring method as shown in Table 1 below. The fragrance test was conducted every two hours and no more than two samples were applied per day.
점수score 냄새강도Odor intensity 부연설명Description
00 없음none 식초인지 알 수 없을 정도로 냄새가 없음Odor not known as vinegar
1One 매우 약함Very weak 식초냄새는 없으나 약간 코를 자극하는 취가 희미하게 감지함No smell of vinegar, but slightly noxious odor
22 약함weakness 식초냄새가 조금 나며 코를 자극하는 취가 감지됨.Vinegar smells a little and irritates the nose.
33 보통usually 식초냄새가 나며 코를 자극하는 취가 바로 감지됨.The smell of vinegar and irritating nose is immediately detected.
44 강함Strong 식초냄새가 즉시 감지되어 일반식초로 인식됨Vinegar odor is detected immediately and recognized as regular vinegar
55 매우 강함Very strong 강한 식초취와 자극이 바로 감지됨 (얼굴을 돌림)Strong vinegar smell and irritation immediately detected (turn face)
(2) 결과(2) results
상기 아세트산이 포함된 향기 농축액의 향취 비교결과를 하기 표 2에 기재하였다.The fragrance comparison results of the fragrance concentrate containing the acetic acid are shown in Table 2 below.
성별gender 번호number 나이age 비교-제조예1AComparative Production Example 1A 비교-제조예1BComparative Production Example 1B 비교-제조예1CComparative Production Example 1C 제조예 1APreparation Example 1A 제조예 1BPreparation Example 1B 제조예 1CPreparation Example 1C 제조예 1DPreparation Example 1D 제조예 1EPreparation Example 1E
female 1One 2424 55 55 55 1One 1One 1One 1One 1One
22 3333 55 55 55 00 1One 22 1One 22
33 3434 55 55 55 1One 1One 1One 1One 1One
44 2121 55 55 55 1One 1One 1One 1One 22
55 2525 55 55 55 1One 00 1One 00 1One
66 3838 55 55 55 1One 1One 00 1One 1One
77 4040 55 55 55 00 1One 1One 1One 1One
male 88 2626 55 55 55 1One 1One 1One 1One 1One
99 3838 55 55 55 1One 1One 22 1One 00
1010 3434 55 55 55 1One 1One 1One 22 1One
1111 2222 55 55 55 1One 22 1One 1One 1One
1212 4343 55 55 55 00 1One 1One 1One 22
1313 3333 55 55 55 1One 1One 22 1One 1One
1414 2525 55 55 55 1One 1One 1One 1One 1One
평균Average 31.131.1 5.05.0 5.05.0 5.05.0 0.90.9 1.21.2 1.11.1 1.21.2 1.31.3
상기 표 2에서 나타난 바와 같이, 향기 농축액을 포함하는 경우 아세트산 취의 감소가 우수한 것을 알 수 있다. 특히 향기 농축액의 원료로 계피를 사용한 제조예 1A는 아세트산 취가 거의 나타나지 않았다.As shown in Table 2, it can be seen that the reduction of acetic acid odor is excellent when the fragrance concentrate is included. In particular, Production Example 1A using cinnamon as a raw material of the fragrance concentrate showed little acetic acid odor.
제조예Production Example 2. 곡물 추출물 제조 2. Grain Extract Manufacturer
쌀을 구입하여 믹서기로 분쇄한 후 분쇄한 쌀 1 g당 10~20 ml의 정제수를 첨가하여 추출하였다. 추출 조건은 70℃에서 정제수에 2 시간 침지 후 동일온도 조건에서 1 시간 교반하여 추출하였다. 추출물은 filter paper (Advantec. Toyo Roshi Kaisha, Japan)를 이용하여 여과한 후 곡물 추출물을 제조하였다. After purchasing the rice was pulverized with a blender and extracted by adding 10 to 20 ml of purified water per 1 g of crushed rice. Extraction conditions were immersed in purified water for 2 hours at 70 ℃ and extracted by stirring for 1 hour at the same temperature conditions. The extract was filtered using a filter paper (Advantec. Toyo Roshi Kaisha, Japan) to prepare a grain extract.
제조예Production Example 3. 발효식초 제조 3. Manufacture of Fermented Vinegar
제조예 1에서 제조된 향기 농축액 및 제조예 2에서 제조된 곡물 추출물을 곡물 추출물: 향기 농축액 = 2:1의 비율로 혼합한 뒤, 발효하여 발효식초를 제조하였다. The fragrance concentrate prepared in Preparation Example 1 and the grain extract prepared in Preparation Example 2 were mixed at a ratio of grain extract: fragrance concentrate = 2: 1, followed by fermentation to prepare fermented vinegar.
이때, 향기성분으로 계피를 사용할 경우 제조예 3A, 아로니아를 사용할 경우 제조예 3B, 히비스커스를 사용할 경우 제조예 3C, 치자를 사용할 경우 제조예 3D 및 백미를 사용할 경우 제조예 3E로 하였다. In this case, when using cinnamon as a scent component, using Preparation Example 3A, using Aaronia Preparation Example 3B, using Hibiscus Preparation Example 3C, using Gardenia Preparation Example 3D and white rice were prepared as Preparation Example 3E.
발효는 하기와 같이 수행하였다. Fermentation was carried out as follows.
(1) 식초발효균주의 관리(1) Management of vinegar fermented strains
Acetobactor aceti를 GYC slant(glucose 50 g/L, yeast extract 10 g/L, CaCO3 30 g/L, agar 15 g/L, pH 6.3)를 보관하고 1개월마다 계대하여 28℃에서 배양하였다.Acetobactor aceti was stored in GYC slant (glucose 50 g / L, yeast extract 10 g / L, CaCO 3 30 g / L, agar 15 g / L, pH 6.3) and passaged every month to incubate at 28 ° C.
(2) 알코올 발효 및 초산 발효(2) alcohol fermentation and acetic acid fermentation
① 알코올 발효배지 조성 및 알코올 발효① Alcohol fermentation medium composition and alcohol fermentation
glucose 0.22 g/L, yeast extract 0.02 g/L, (NH4)2HPO4 0.14 g/L, KH2PO4 0.014 g/L, MgSO4(7H2O) 0.002 g/L, 곡물 추출물 300 ml/L, 향기 농축액 150 ml/L, 발효주정 100 g/L의 조성을 가지는 알코올 발효배지를 제조하였다. glucose 0.22 g / L, yeast extract 0.02 g / L, (NH 4 ) 2 HPO 4 0.14 g / L, KH 2 PO 4 0.014 g / L, MgSO 4 (7H 2 O) 0.002 g / L, grain extract 300 ml Alcohol fermentation medium having a composition of / L, fragrance concentrate 150 ml / L, fermentation alcohol 100 g / L was prepared.
상기 알코올 발효배지에 30℃ 및 130 rpm 조건에서 진탕배양한 효모를 원심분리하고 분리된 효모를 OD(Optical Density)값 2에 맞추어 0.5% (w/v) 접종하여 30℃에서 3~7일간 배양하였다. 그 후 배양액을 가압제균 (<5PSI, 0.45 μm) 처리하였다. Centrifuge the yeast shaken at 30 ℃ and 130 rpm in the alcohol fermentation medium and inoculated 0.5% (w / v) in accordance with the OD (Optical Density) value 2 incubated at 30 ℃ for 3-7 days It was. The culture was then treated with autoclaving (<5 PSI, 0.45 μm).
② 초산 발효② acetic acid fermentation
접종용(초산균주) 배양배지는 glucose 1.1 g/L, yeast extract 0.2 g/L, peptone 10 g/L, (NH4)2HPO4 1.4 g/L, KH2PO4 0.14 g/L, MgSO4(7H2O) 0.02 g/L, acetic acid(빙초산) 21 g/L, ethanol(95%) 42 g/L의 조성을 가지도록 제조하여 사용하였으며, 휘발성 물질인 에탄올은 배지 멸균 후 0.22 PVDF 필터로 여과하여 첨가하였다. Culture medium for inoculation (acetic acid) was 1.1 g / L glucose, 0.2 g / L yeast extract, 10 g / L peptone, (NH 4 ) 2 HPO 4 1.4 g / L, KH 2 PO 4 0.14 g / L, MgSO 4 (7H 2 O) 0.02 g / L, acetic acid 21 g / L, ethanol (95%) 42 g / L was used to prepare the composition, volatile ethanol was 0.22 PVDF filter after media sterilization It was added by filtration.
접종용 균주 배양은 300 ml baffle flask에 접종용 배양배지 50 ml을 넣고 멸균 후 루프로 균(초산균)을 접종하여 진탕배양기에서 200 rpm, 30℃에서 48시간 배양하였다.Strain culture for inoculation was put 50 ml of inoculation culture medium in a 300 ml baffle flask, sterilized and inoculated with bacteria (acetic acid) in a loop and incubated for 48 hours at 200 rpm, 30 ℃ in a shaker.
또한, (2)의 ①에서 배양되고 가압제균 처리된 배지를 3.5리터 넣고 Seed vinegar (초산균 108 cells/ml)를 945 ml을 넣고 0 rpm, 통기 1 VVM, 30℃에서 7일 간 배양하여 수행하여 발효식초를 제조하였다.In addition, 3.5 liters of medium cultured in ① of (2) and autoclave-treated and put 945 ml of Seed vinegar (108 cells / ml) were incubated at 0 rpm, aeration 1 VVM, 30 ° C. for 7 days. Fermented vinegar was prepared.
실험예Experimental Example 2. 발효식초의 아세트산 취 평가 2. Acetic Acid Odor Evaluation of Fermented Vinegar
(1) 발효식초 취 측정 방법(1) fermentation vinegar odor measurement method
제조예 3에서 제조된 발효식초에 정제수를 첨가하여 최종 아세트산의 함량을 약 6%로 조정한 후 향취를 평가하였다. Purified water was added to the fermented vinegar prepared in Preparation Example 3 to adjust the content of the final acetic acid to about 6% and the odor was evaluated.
이때, 비교-제조예 3A로 정제수에 아세트산을 6% (g/v) 혼합한 혼합물을 사용하였고, 비교-제조예 3B로 아세트산 6% (g/v)와 에탄올 10% 를 혼합한 후 발효한 발효물을 사용하였으며, 비교-제조예 3C로 곡물 추출물과 에탄올 10%를 혼합한 후 발효한 발효물을 사용하였다. In this case, a mixture of acetic acid 6% (g / v) was added to purified water in Comparative Preparation Example 3A, and 6% (g / v) of acetic acid and 10% of ethanol were mixed in Comparative Preparation Example 3B, followed by fermentation. Fermented product was used, and fermented product was used after mixing grain extract and ethanol 10% in Comparative Preparation Example 3C.
상기 취 측정은 전술한 실험예 1의 향취 측정과 같은 방법으로 수행하였다. The odor measurement was performed by the same method as the odor measurement of Experimental Example 1 described above.
(2) 결과(2) results
상기 발효식초의 취 비교결과를 하기 표 3에 기재하였다.Table 3 shows the results of comparing the odor of the fermented vinegar.
성별gender 번호number 나이age 비교-제조예3AComparative Production Example 3A 비교-제조예3BComparative Production Example 3B 비교-제조예3CComparative Production Example 3C 제조예 3APreparation Example 3A 제조예 3BPreparation Example 3B 제조예 3CPreparation Example 3C 제조예 3DPreparation Example 3D 제조예 3EPreparation Example 3E
HPLC 아세트산 분석결과HPLC Acetic Acid Assay 5.9985.998 8.2078.207 7.4257.425 8.0648.064 7.837.83 7.8847.884 8.5278.527 7.2647.264
female 1One 2424 55 33 44 1One 1One 1One 1One 1One
22 3333 55 44 55 1One 1One 1One 1One 22
33 3434 55 55 44 22 22 22 1One 1One
44 2121 55 55 55 1One 1One 1One 22 22
55 2525 55 44 44 1One 1One 1One 1One 1One
66 3838 55 55 55 1One 22 22 1One 1One
77 4040 55 44 33 22 1One 1One 1One 22
male 88 2626 55 55 55 1One 1One 1One 1One 1One
99 3838 55 55 55 1One 22 1One 1One 1One
1010 3434 55 33 33 1One 1One 22 22 22
1111 2222 55 55 55 22 1One 1One 1One 1One
1212 4343 55 44 44 1One 22 22 1One 22
1313 3333 55 55 55 22 1One 1One 22 1One
1414 2525 55 44 55 1One 1One 1One 1One 22
평균Average 31.131.1 5.05.0 4.44.4 4.44.4 1.31.3 1.31.3 1.31.3 1.21.2 1.41.4
상기 표 3에 나타난 바와 같이, 향기 농축액을 포함하는 발효식초는 아세트산 취의 감소가 우수한 것을 알 수 있다.As shown in Table 3, it can be seen that the fermentation vinegar containing the fragrance concentrate is excellent in the reduction of acetic acid odor.
제조예Production Example 4.  4. 향기 나는Fragrant 식초 제조 Vinegar manufacturer
(1) 향기 추출액 제조(1) scent extract
건조된 식물성 원재료, 즉 향기성분으로 계피, 아로니아, 히비스커스, 치자 또는 백미를 사용하였다.Dried vegetable raw material, that is, cinnamon, aronia, hibiscus, gardenia or white rice was used as a fragrance component.
향기성분을 믹서로 파쇄한 후, 추출용매로 정제수를 첨가하였다. 이때 추출용매의 양은 파쇄된 원재료의 건조중량 1 g 당 10~20 ml로 하였으며 추출조건은 10℃에서 10시간 침지 후 동일 온도조건에서 3시간, 150 rpm, -500 mmHg 교반/감압 추출하였다. 추출물은 filter paper (Advantec. Toyo Roshi Kaisha, Japan)로 여과하여 향기 추출액을 제조하였다.After crushing the fragrance components with a mixer, purified water was added as an extraction solvent. At this time, the amount of the extraction solvent was 10 ~ 20 ml per 1 g of the dry weight of the crushed raw material and extraction conditions were extracted for 3 hours, 150 rpm, -500 mmHg stirring / decompression at the same temperature conditions after 10 hours immersion at 10 ℃. The extract was filtered through a filter paper (Advantec. Toyo Roshi Kaisha, Japan) to prepare a fragrance extract.
(2) 향기 추출액 혼합(2) mixed fragrance extract
(1)에서 제조된 향기 추출액을 발효식초에 첨가하였다. The fragrance extract prepared in (1) was added to the fermented vinegar.
이때, 향기 추출액은 10% (w/v) 함량비로 발효식초에 첨가하였다. At this time, the fragrance extract was added to the fermented vinegar in a content ratio of 10% (w / v).
(3) 자연숙성발효 및 향기 나는 식초 제조(3) Natural ripening fermentation and scented vinegar manufacture
상기 (2)의 혼합액을 10℃에서 7 일간 숙성하였다. The mixed solution of (2) was aged at 10 ° C. for 7 days.
즉, 향기 나는 식초의 제조 방법의 제조 방법은 도 1의 방법에 따라 제조될 수 있다. That is, the manufacturing method of the manufacturing method of the scented vinegar may be manufactured according to the method of FIG.
실험예Experimental Example 3.  3. 향기 나는Fragrant 식초 조성물의 취 비교  Odor Comparison of Vinegar Compositions
(1) 향기 나는 식초 취 측정 방법(1) How to measure the smell of vinegar
제조예 4에서 제조된 향기 나는 식초 조성물의 취를 평가하였다.The smell of the scented vinegar composition prepared in Preparation Example 4 was evaluated.
이때, 향기 나는 식초에 사용된 향기 농축액 및 향기 추출액의 향기성분을 하기 표 4에 기재하였다. At this time, the fragrance components of the fragrance concentrate and fragrance extract used in the scented vinegar are listed in Table 4 below.
비교-제조예4AComparative Production Example 4A 비교-제조예4BComparative Production Example 4B 비교-제조예4CComparative Production Example 4C 제조예4APreparation Example 4A 제조예 4BPreparation Example 4B 제조예 4CPreparation Example 4C 제조예 4DPreparation Example 4D 제조예 4EPreparation Example 4E
향기 농축액 향기성분Fragrance Concentrate
계피cinnamon XX XX XX OO XX XX XX XX
아로니아Aaronia XX XX XX XX OO XX XX XX
히비스커스hibiscus XX XX XX XX XX OO XX XX
치자Gardenia XX XX XX XX XX XX OO XX
백미White rice XX XX XX XX XX XX XX OO
장미허브Rose herb XX XX XX XX XX XX XX XX
향기 추출액 향기성분Fragrance extract
계피cinnamon XX XX XX OO XX XX XX XX
아로니아Aaronia XX XX XX OO OO OO XX XX
히비스커스hibiscus XX XX XX XX XX XX XX XX
치자Gardenia XX XX OO OO OO OO OO OO
백미White rice XX XX XX XX XX XX XX OO
장미허브Rose herb XX XX XX XX XX XX XX XX
비교-제조예 4A로 정제수에 아세트산을 6% (g/v) 혼합한 혼합물을 사용했고, 비교-제조예 4B로 아세트산 6% (g/v)와 에탄올 10%을 혼합한 후 발효한 발효물을 숙성발효하여 사용했으며, 비교-제조예 4C로 곡물 추출물과 에탄올 10%를 혼합한 후 발효하고, 향기 추출액(치자 추출액)을 혼합한 뒤 숙성발효하여 사용하였다.In Comparative Preparation 4A, a mixture of acetic acid 6% (g / v) was used in purified water, and in Comparative Preparation 4B, fermentation was made after mixing 6% (g / v) of acetic acid and 10% of ethanol. It was used by fermentation, fermented after mixing grain extract and ethanol 10% in Comparative Preparation Example 4C, and mixed with the fragrance extract (garia extract) and then used by fermentation.
상기 취 측정은 전술한 실험예 1의 향취 측정과 같은 방법으로 수행하였다.The odor measurement was performed by the same method as the odor measurement of Experimental Example 1 described above.
(2) 결과(2) results
상기 향취 비교결과를 하기 표 5에 기재하였다.The odor comparison results are shown in Table 5 below.
성별gender 번호number 나이age 비교-제조예4AComparative Production Example 4A 비교-제조예4BComparative Production Example 4B 비교-제조예4CComparative Production Example 4C 제조예4APreparation Example 4A 제조예 4BPreparation Example 4B 제조예 4CPreparation Example 4C 제조예 4DPreparation Example 4D 제조예 4EPreparation Example 4E
향기농축액첨가/발효Fragrance concentrate addition / fermentation xx xx xx oo oo oo oo oo
향기추출액첨가/숙성Fragrance Extract Addition / Maturation xx xx oo oo oo oo oo oo
female 1One 2424 55 33 33 1One 00 1One 00 1One
22 3333 55 44 22 00 1One 00 1One 00
33 3434 55 55 22 00 00 1One 00 1One
44 2121 55 55 1One 1One 00 1One 1One 1One
55 2525 55 44 22 00 00 00 00 00
66 3838 55 55 22 1One 1One 1One 00 1One
77 4040 55 44 33 00 00 00 1One 00
male 88 2626 55 55 22 1One 00 1One 00 1One
99 3838 55 55 1One 00 1One 00 1One 00
1010 3434 55 33 33 00 00 1One 00 00
1111 2222 55 55 22 1One 00 00 00 1One
1212 4343 55 44 33 00 1One 00 1One 00
1313 3333 55 55 22 1One 00 1One 00 00
1414 2525 55 44 22 1One 1One 1One 1One 1One
평균Average 31.131.1 5.05.0 4.44.4 2.12.1 0.50.5 0.40.4 0.60.6 0.40.4 0.580.58
상기 표 5에 나타난 바와 같이, 향기 농축액이 첨가된 발효식초에 향기 추출액을 혼합할 경우 아세트산 취의 감소 효과가 더욱 우수한 것을 확인할 수 있다.As shown in Table 5, it can be seen that the effect of reducing the acetic acid odor when mixing the fragrance extract with fermented vinegar to which the fragrance concentrate is added.
본 발명에 따른 향기 나는 식초 조성물은 그 자체로 세정제로 사용가능하며, 린스, 섬유유연제 및 탈취제 등의 용도로 사용 가능하다.The scented vinegar composition according to the present invention can be used as a cleaning agent by itself, and can be used as a rinse, a fabric softener, and a deodorant.

Claims (13)

  1. 곡물 추출물 및 향기성분 농축액의 혼합물을 발효하여 발효식초를 제조하는 단계를 포함하는 향기 나는 식초 조성물의 제조 방법. A method for producing a fragrant vinegar composition comprising the step of fermenting a mixture of grain extract and a concentrated component concentrate to prepare fermented vinegar.
  2. 제 1 항에 있어서,The method of claim 1,
    곡물은 쌀, 보리, 밀 또는 이들의 혼합물인 향기 나는 식초 조성물의 제조 방법. A method for producing a fragrant vinegar composition wherein the grain is rice, barley, wheat or mixtures thereof.
  3. 제 1 항에 있어서,The method of claim 1,
    곡물 추출물은 곡물의 열수, 에탄올 또는 감압추출물인 향기 나는 식초 조성물의 제조 방법. Cereal extract is a method of producing a fragrant vinegar composition which is hot water, ethanol or reduced pressure extract of grain.
  4. 제 1 항에 있어서, The method of claim 1,
    향기성분은 계피, 아로니아, 히비스커스, 치자, 백미, 레몬밤, 캐모마일, 페퍼민트, 스피아민트, 세이지, 벨가못, 바질, 타임, 오레가노, 아카시아꽃, 백합, 연꽃, 자스민, 장미, 라벤터, 국화, 라일락, 살구, 후리지아, 튤립, 유칼립튜스, 로즈마리, 라일락, 목련, 애플민트, 티트리, 히아신스, 벚꽃, 레몬버베나, 동백, 훤넬, 복숭아꽃, 블루베리, 라즈베리 또는 이들의 혼합물인 향기 나는 식초 조성물의 제조 방법. Flavoring ingredients include cinnamon, aronia, hibiscus, gardenia, white rice, lemon balm, chamomile, peppermint, spearmint, sage, bergamot, basil, thyme, oregano, acacia, lily, lotus, jasmine, rose, lavender, chrysanthemum Fragrant, lilac, apricot, freesia, tulip, eucalyptus, rosemary, lilac, magnolia, applemint, tea tree, hyacinth, cherry blossom, lemon verbena, camellia, chanel, peach blossom, blueberry, raspberry or mixtures thereof Process for the preparation of the vinegar composition.
  5. 제 1 항에 있어서,The method of claim 1,
    향기성분 농축액은 향기성분의 냉침, 저온, 열수, 감압 또는 에탄올 추출물의 농축물인 향기 나는 식초 조성물의 제조 방법. Aromatic component concentrate is a method of producing a scented vinegar composition which is a concentrate of cold, low temperature, hot water, reduced pressure or ethanol extract of the aromatic component.
  6. 제 1 항에 있어서, The method of claim 1,
    향기성분 농축액의 함량은 곡물 추출물 100 중량부에 대하여 20 내지 100 중량부인 향기 나는 식초 조성물의 제조 방법. A method of producing a fragrant vinegar composition is 20 to 100 parts by weight based on 100 parts by weight of grain extract.
  7. 제 1 항에 있어서, The method of claim 1,
    발효는 곡물 추출물 및 향기성분 농축액의 혼합물을 에탄올 발효한 뒤, 초산 발효하는 것인 향기 나는 식초 조성물의 제조 방법.Fermentation is a method of producing a fragrant vinegar composition is a fermentation of acetic acid after fermentation of a mixture of grain extract and flavor component concentrate.
  8. 제 1 항에 있어서, The method of claim 1,
    발효식초에 향기성분 추출액을 혼합하는 단계를 추가로 포함하는 향기 나는 식초 조성물의 제조 방법. Method for producing a fragrant vinegar composition further comprising the step of mixing the fragrance component extract with fermented vinegar.
  9. 제 8 항에 있어서, The method of claim 8,
    향기성분 추출액에서 향기성분은 계피, 아로니아, 히비스커스, 치자, 백미, 레몬밤, 캐모마일, 페퍼민트, 스피아민트, 세이지, 벨가못, 바질, 타임, 오레가노, 아카시아꽃, 백합, 연꽃, 자스민, 장미, 라벤터, 국화, 라일락, 살구, 후리지아, 튤립, 유칼립튜스, 로즈마리, 라일락, 목련, 애플민트, 티트리, 히아신스, 벚꽃, 레몬버베나, 동백, 훤넬, 복숭아꽃, 블루베리, 라즈베리 또는 이들의 혼합물인 향기 나는 식초 조성물의 제조 방법. The fragrance components in the fragrance extract are cinnamon, aronia, hibiscus, gardenia, white rice, lemon balm, chamomile, peppermint, spearmint, sage, belgium, basil, thyme, oregano, acacia, lily, lotus, jasmine, rose, Lavender, chrysanthemum, lilac, apricot, freesia, tulip, eucalyptus, rosemary, lilac, magnolia, applemint, tea tree, hyacinth, cherry blossom, lemon verbena, camellia, chanel, peach blossom, blueberry, raspberry or their A method for producing a fragrant vinegar composition which is a mixture.
  10. 제 8 항에 있어서, The method of claim 8,
    향기성분 추출액은 향기성분의 냉침, 저온, 열수, 감압 또는 에탄올 추출물인 향기 나는 식초 조성물의 제조 방법.The fragrance component extract is a cold vinegar, low temperature, hot water, reduced pressure or ethanol extract of the fragrance component manufacturing method of the scented vinegar composition.
  11. 제 8 항에 있어서,The method of claim 8,
    향기성분 추출액의 함량은 발효식초 100 중량부에 대하여 0.5 내지 20 중량부인 향기 나는 식초 조성물의 제조 방법. The content of the fragrance component extract is 0.5 to 20 parts by weight based on 100 parts by weight of the fermented vinegar method of producing a fragrant vinegar composition.
  12. 제 8 항에 있어서, The method of claim 8,
    발효식초 및 향기성분 추출액의 혼합물을 숙성하는 단계를 추가로 포함하는 향기 나는 식초 조성물의 제조 방법. A method of producing a fragrant vinegar composition further comprising the step of ripening a mixture of the fermented vinegar and the flavor component extract.
  13. 제 1 항의 제조 방법에 의해 제조된 향기 나는 식초 조성물. A fragrant vinegar composition prepared by the method of claim 1.
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US11072768B2 (en) 2018-11-07 2021-07-27 The Procter & Gamble Company Low pH fabric care compositions
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KR101958155B1 (en) * 2018-12-19 2019-03-13 송소현 Method for manufacturing eco-friendly deodorization and detergent composite
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