WO2018033165A1 - 一种高分子-金属氧化物复合物及其制备方法与应用 - Google Patents
一种高分子-金属氧化物复合物及其制备方法与应用 Download PDFInfo
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- WO2018033165A1 WO2018033165A1 PCT/CN2017/106676 CN2017106676W WO2018033165A1 WO 2018033165 A1 WO2018033165 A1 WO 2018033165A1 CN 2017106676 W CN2017106676 W CN 2017106676W WO 2018033165 A1 WO2018033165 A1 WO 2018033165A1
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- metal oxide
- iron
- polyacrylic acid
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- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5138—Organic macromolecular compounds; Dendrimers obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/51—Nanocapsules; Nanoparticles
- A61K9/5107—Excipients; Inactive ingredients
- A61K9/513—Organic macromolecular compounds; Dendrimers
- A61K9/5146—Organic macromolecular compounds; Dendrimers obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F292/00—Macromolecular compounds obtained by polymerising monomers on to inorganic materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Definitions
- the invention relates to the field of nanotechnology and biomedical engineering technology, in particular to a polymer-metal oxide composite.
- superparamagnetic nanoparticles represented by iron oxide have been widely used in various fields of biomedical science due to their unique physical and chemical properties, such as biomagnetic separation, targeted drug delivery, gene transfection, immunodiagnosis, and deficiency.
- Iron anemia treatment enhanced magnetic resonance imaging and other fields.
- nanometer-sized iron oxide particles have been widely studied and applied in the treatment of superparamagnetic magnetic resonance contrast agents and iron deficiency anemia.
- the molecular structure of iron oxide nanoparticles in the above applications is mainly a polymer complex of triiron tetroxide or ferric oxide, and commercial superparamagnetic iron oxide complex molecular contrast agents mainly include Combidex, Resovist and Feridex.
- the molecular structure of these three types of iron oxide complexes is: the center is a triiron tetroxide crystal particle, and the surface is chelated by a hydroxyl group-coordinated iron atom on a hydrophilic polymer (such as dextran), thereby making this
- the iron oxide-like complex can be dispersed in an aqueous solution;
- the commercial iron oxide nanoparticle as a iron deficiency anemia iron-containing agent mainly has Ferumoxtol, and adopts a molecular structure in which a carboxylated modified glucan is chelated with an iron atom.
- the final particle size is 60-150 nm.
- the preparation method has the advantages of simple method, simple operation and easy mass production.
- the coprecipitation method has a faster reaction rate, and the nucleation and crystallization processes are difficult to separate, resulting in poor monodispersity of the particles and wide particle size distribution, which requires further sorting to obtain a desired particle size.
- the reaction medium of the coprecipitation method is an aqueous phase
- the reaction temperature is lower than 100 ° C, and the lower reaction temperature makes the crystallinity of the central iron oxide crystals lower, resulting in weak magnetization and poor actual contrast effect.
- the high-temperature thermal decomposition method can reach nano-particles with narrow particle size distribution and higher crystallinity due to high reaction temperature.
- the nano-particles obtained by the traditional high-temperature thermal decomposition method are generally oil-soluble, which is not conducive to further biological applications.
- the aqueous phase nanoparticles using polyol as a stabilizer have a small amount of surface modification, and are prone to poor agglomeration stability when applied to in vivo angiography, which seriously affects the blood circulation cycle, and the imaging effect is not satisfactory.
- US Patent No. 6,599,498 B1 discloses a molecular structure of a modified superparamagnetic iron oxide complex using carboxydextran as a coordination polymer, which uses carboxydextran as a surface modifying polymer to The coordination capacity of the central iron atom is enhanced, and at the same time, the free iron ion oozing is reduced during application, which alleviates the hypersensitivity reaction in clinical use [VS Balakrishnan et al, Eur. J. Clin. Invest. 39 (2009) 489.].
- the molecular structure still does not achieve complete chelation of iron atoms on the surface of iron oxide, and there is still a problem of hypersensitivity reaction of dispersion stability and free iron ion exudation in clinical application.
- CN 103347543 A discloses a molecular structure of an iron oxide composite coated with a hydrophilic material centered on high crystallinity iron oxide particles, the surface of which is coupled with carboxymethyldextran by ligand exchange.
- the nanoparticle is a hydrophobic surface and cannot be used for biological applications, further ligand exchange is required, that is, the hydrophilic polymer ligand is used to convert the nanoparticles into hydrophilic nanoparticles.
- This method has a long cycle, and due to the use of ligand exchange, the chelation between the surface ligand and the iron ion is weak, and it is easy to fall off and release free iron ions to cause a hypersensitivity reaction.
- CN101002951A discloses a method for preparing hydrophilic iron oxide complex molecules by a polyol method, which can prepare hydrophilic, monodisperse and high crystallinity iron oxide complex molecules relatively easily, but the surface organic matter content is small. Therefore, its physiological stability is poor, and its blood circulation characteristics in the living body cannot be guaranteed.
- the molecular structure design of the complex and its key problems in vivo application are related to the application effect of iron oxide complex as a magnetic resonance contrast agent and iron deficiency iron supplement in vivo, and also whether it will release freely in the body. Iron ions and the resolution of safety issues such as hypersensitivity reactions.
- the present invention provides a polymer-metal oxide composite comprising metal oxide particles at the core and modified in the metal oxide a polymer on the surface of the particle, the polymer having a functional group capable of bonding to a metal in the metal oxide, and a density of a surface binding site of the polymer and the metal oxide particle is higher than 2 sites/ Square nanometer;
- the polymer-metal oxide composite contains one or more metal elements such as iron, cobalt, nickel, iron cobalt, iron nickel, and the like.
- the polymer is selected from one or more of polyacrylic acid, polyacrylic acid salt, methyl polyacrylic acid, methyl polyacrylate, polylactic acid, polylactic acid salt, and polyphosphate.
- the polymer has a weight average molecular weight of 500 to 500,000 Da.
- the polymer has a weight average molecular weight of 500 to 3000 Da.
- the lower molecular weight polymer-metal oxide composite is less toxic to organisms and has better biocompatibility when used as an injection.
- the total mass of the polymer accounts for 25% to 70% of the polymer-metal oxide composite.
- the total mass of the polymer accounts for 40% to 70% of the polymer-metal oxide composite.
- the metal oxide is selected from one or more of the group consisting of iron oxide, manganese oxide, cobalt oxide, chromium oxide, and nickel oxide.
- the metal oxide is iron oxide.
- the polymer-metal oxide composite is a polyacrylic acid-iron oxide complex, and a density of surface binding sites of the polyacrylic acid and the iron oxide particles is higher than 2 sites/square nanometer;
- the central iron oxide particles have a particle diameter of 1 to 30 nm under a transmission electron microscope.
- the surface-coupled polymer is a low molecular weight polyacrylic acid
- the polyacrylic acid has a weight average molecular weight of 1000-10000
- the total mass of the polyacrylic acid accounts for 25%-70% of the complex molecule.
- the polyacrylic acid-iron oxide complex consists of a high crystallinity iron oxide center and a high proportion of surface carboxyl polymer, and the novel molecular structure gives the polyacrylic acid-iron oxide complex a high degree of hydrophilicity and physiology.
- High dispersion stability in saline solution, good chelating properties with free and surface iron ions, excellent magnetic resonance relaxation enhancement properties and iron metabolism properties, these properties make these new polyacrylic acid-iron oxide complexes applicable Magnetic resonance imaging contrast agents in tissues or cells such as blood vessels, liver, spleen, lymph, and heart, and iron deficiency anemia iron supplements.
- the invention also provides a preparation method of the above polymer-metal oxide composite, the preparation method comprising the following steps:
- Step 1 the precipitating agent is dissolved in a reducing solvent to form a solution B;
- Step 2 dissolving the polymer in the reducing solvent
- Step 3 weigh the metal salt, the metal salt is dissolved in the mixed solution obtained in step 2, formulated into solution A;
- Step 4 the solution A and the solution B are reacted under microwave conditions; cooling to obtain a polymer-metal oxide complex molecular colloid;
- Step 5 separating and removing the polymer-metal oxide complex molecular colloid obtained in the step 4 to remove impurities (excluding impurities mainly means removing a solvent, a heavy metal or an unreacted polymer, etc.) to obtain the polymer- Metal oxide composite.
- impurities excluding impurities mainly means removing a solvent, a heavy metal or an unreacted polymer, etc.
- the reducing solvent is a hydrophilic high boiling point solvent, and the reducing solvent has a boiling point of 180 ° C or higher.
- the reducing solvent is selected from one or more of diethylene glycol, ethylene glycol, propylene glycol, glycerin, propylene glycol, and diethylene glycol.
- the precipitating agent is sodium hydroxide, sodium acetate or sodium borohydride.
- the polymer is selected from one or more of polyacrylic acid, polyacrylic acid salt, methyl polyacrylic acid, methyl polyacrylate, polylactic acid, polylactic acid salt, and polyphosphate.
- the metal salt is one or more selected from the group consisting of ferric chloride, iron sulfate, iron hydroxide, iron acetylacetonate, and iron cobalt acetylacetonate.
- reaction temperature under the microwave conditions is from 180 ° C to 280 ° C.
- reaction time under the microwave conditions is from 5 min to 30 min.
- the method for preparing the polyacrylic acid-iron oxide composite comprises the following steps:
- Step 1 the precipitating agent is dissolved in a reducing solvent to form a solution B;
- Step 2 dissolving polyacrylic acid in the reducing solvent
- Step 3 the iron salt is dissolved in the mixed solution obtained in step 2, formulated into solution A;
- Step 4 the solution A and the solution B are reacted under microwave conditions; cooling to obtain a polyacrylic acid-iron oxide complex molecular colloid;
- Step 5 separating and separating the polyacrylic acid-iron oxide complex molecular colloid obtained in the step 4 to obtain a polyacrylic acid-iron oxide complex.
- the present invention also provides a nuclear magnetic resonance contrast agent comprising the above-described polymer-metal oxide composite containing iron element.
- the nuclear magnetic resonance contrast agent can perform enhanced T1, T2 and T2* magnetic resonance imaging on normal or diseased blood vessels, liver, spleen, lymph, heart and other organs or tissues.
- the nuclear magnetic resonance contrast agent is an injection or an oral preparation.
- the invention also provides the use of the above nuclear magnetic resonance contrast agent in tissue or nuclear magnetic development.
- tissue or cell is a blood vessel, a liver, a spleen lymph or a heart.
- the present invention also provides a iron-reinforcing agent comprising the above-mentioned polymer-metal oxide composite containing iron element.
- the iron supplement can rapidly increase the levels of hemoglobin and transferrin in the blood.
- iron supplement is an injection or an oral preparation.
- Oral formulations include capsules, tablets and the like.
- the invention also provides the application of the above iron supplement in the preparation of a medicament for treating iron deficiency anemia.
- the polymer-metal oxide composite provided by the invention contains a large amount of polyelectrolyte polymer, has good dispersion stability, uniform particle size distribution, high crystallinity of the central metal oxide, good contrast effect and long cycle function. .
- the uniformity of microwave heating is good, the heating efficiency is high, the synthesis process is greatly shortened, and the preparation cost is reduced.
- the invention provides a novel molecular structure of a polymer-metal oxide, especially a polyacrylic acid-iron oxide complex, and a preparation thereof for the problem that the surface iron atom has low chelation density and weak bonding strength in the prior iron oxide composite.
- Methods, and such complexes are prepared in a variety of dosage forms for use in magnetic resonance contrast agents and iron supplements for the treatment of iron deficiency anemia. It greatly extends the polyacrylic acid-iron oxide complex in vivo The time of the ring effectively overcomes the shortcomings of the existing iron oxide complex injection with hypersensitivity reaction, plus the superparamagnetism of the complex itself and the function of participating in iron metabolism, enabling it to be applied to magnetic resonance imaging contrast agents. And iron supplements for the treatment of iron deficiency anemia.
- Figure 1 is a graph showing thermogravimetric analysis of four polyacrylic acid-iron oxide complex molecules in a preferred embodiment of the present invention
- Figures 1a to 1d respectively show polyacrylic acid-oxidation in Examples 1-4.
- FIG. 2 is a transmission electron microscope (TEM) photograph particle size distribution diagram of four polyacrylic acid-iron oxide complex molecules in a preferred embodiment of the present invention
- FIGS. 2a to 2d respectively show examples 1-4.
- Figure 3 shows the saturation magnetization curves of four polyacrylic acid-iron oxide complex molecules in a preferred embodiment of the invention
- Figure 3a shows the four polyacrylic acid-iron oxide complexes of Examples 1-4.
- the saturation magnetization curve of the molecule Figure 3b shows an enlarged view of the curve near zero;
- Figure 4 shows the relaxation time curves of four polyacrylic acid-iron oxide complex molecules in a preferred embodiment of the invention
- Figures 4a, 4b show the polyacrylic acid-iron oxide complexes of Examples 1-4.
- Figure 5 is a graph showing the sterilization stability curves of four polyacrylic acid-iron oxide complex molecular injection agents in a preferred embodiment of the present invention.
- Figure 6 is a graph showing the stability curves of four polyacrylic acid-iron oxide complex molecular injection agents in a preferred embodiment of the present invention.
- Figure 7 is a diagram showing normal liver magnetic resonance imaging of different concentrations of polyacrylic acid-iron oxide complex molecular injection in a preferred embodiment of the present invention.
- Figure 8 is a graph showing a contrast-enhanced magnetic resonance imaging of a polyacrylic acid-iron oxide complex molecular injection agent and a commercial sputum contrast agent in a normal blood vessel in a preferred embodiment of the present invention
- Figure 9 is a view showing a magnetic resonance image of a diseased liver tissue (liver cancer) of a polyacrylic acid-iron oxide complex molecular injection in a preferred embodiment of the present invention.
- Figure 10 is a magnetic resonance imaging diagram of a diseased blood vessel (aneurysm) of a polyacrylic acid-iron oxide complex molecular injection in a preferred embodiment of the present invention
- Figure 10a Aneurysm model rabbit left common carotid artery bridging hemangioma (shown at the head of the arrow) clearly shows that the aneurysm model has a positive view of the middle part of the common carotid artery in the left common carotid artery.
- the diameter of the hemangioma is significantly larger than that of the common carotid artery, which is significantly different from the contralateral normal carotid artery.
- Figure 10b removes the rest. After the blood vessels, the aneurysm is more obvious;
- the lateral view of the aneurysm in Fig. 10c and Fig. 10d is highly consistent with the gross pathological morphology of the aneurysm taken during surgery;
- Figure 11 is a view showing the magnetic resonance image of the coronary artery of the polyacrylic acid-iron oxide complex molecular injection in the preferred embodiment of the present invention
- Figure 11a shows the imaging result of the 15 minute coronary artery (shown by the dotted line), coronary artery Can be clearly displayed
- Figure 11b shows the results of coronary artery (shown in dashed lines) after 180 minutes, the coronary artery information has almost disappeared
- Figure 11c shows the imaging results of the 15min coronary anterior descending artery (shown by the dotted line), the anterior descending coronary artery can be obvious
- Figure 11d shows the results of a 15-minute coronary circumflex artery (shown in phantom), and the coronary circumflex can be clearly displayed;
- Figure 12 is a schematic view showing the possible structure of a polyacrylic acid-iron oxide complex molecule in a preferred embodiment of the present invention.
- a solution A (the solution is brown); finally, the solution A is placed in a three-necked flask at a constant temperature of 220 ° C for 10 min in a microwave reactor, and then rapidly added to the hot solution B. The reaction is carried out instantaneously and the temperature is kept for 10 minutes. After cooling, a polyacrylic acid-iron oxide complex molecular colloid is obtained, and then 4 L of ultrapure water is added for ultrafiltration washing, and the composite is spray-dried to obtain a polyacrylic acid-ferric oxide complex molecule.
- the measuring tube is used to measure 100 ml of propylene glycol, and then 15 g of sodium borohydride is weighed by a precision balance, dissolved in propylene glycol by ultrasonication, heating and stirring to prepare a solution B, and the solution B is placed in a 70 ° C oven for constant temperature;
- solution A is prepared (the solution is brown); finally, solution A is placed in a three-necked flask at a constant temperature of 240 ° C for 20 min in a microwave reactor, and then hot solution B is quickly added, and the reaction is instantaneously carried out. Constant temperature for 30min. Thereafter, cooling was carried out to obtain a polyacrylic acid-iron oxide complex molecular colloid, followed by precipitation with ethyl acetate and ethanol, and the precipitated complex was washed three times, and finally dispersed in water to freeze-dry to obtain a polyacrylic acid-ferric oxide complex molecule.
- a solution A (the solution is brown); finally, the solution A is placed in a three-necked flask at a constant temperature of 200 ° C for 20 min in a microwave reactor. Then, the hot solution B was quickly added, and the reaction was carried out instantaneously, and the temperature was kept for 30 minutes. Thereafter, cooling was carried out to obtain a sodium colloid-iron oxide complex molecular colloid, followed by precipitation with ethyl acetate, and the precipitated complex was washed three times to obtain a black sodium polyacrylate-ferric oxide complex solution. The obtained sodium polyacrylate-ferric oxide complex solution was heated to 80 ° C, and air was introduced into the system for 4 hours to obtain a reddish brown sodium polyacrylate-ferric oxide complex solution to make the composite. more stable.
- Constant temperature secondly, weighed 6 g of sodium polyacrylate solution (45%) with a weight average molecular weight of 1200 Da in a beaker, weighed 300 ml of glycerin in a beaker, stirred and sonicated to dissolve the two; quickly weighed iron acetylacetonate 2g and 1g of acetylacetone iron manganese, dissolved in a mixture of glycerol and sodium polyacrylate by heating, sonication, stirring, to form solution A (the solution is brown); finally, solution A is placed in a three-necked flask in the microwave The reactor was thermostated at 220 ° C for 20 min, then the hot solution B was quickly added, and the reaction was carried out instantaneously, and the temperature was kept for 30 min.
- Constant temperature Secondly, weigh the weight 5.6g of polylactic acid with a molecular weight of 10000Da was placed in a beaker, then weighed 300ml of diethylene glycol in a beaker, stirred and sonicated to dissolve the two; quickly weigh 2g of anhydrous ferric chloride, and heated, sonicated and stirred Dissolved in a mixture of diethylene glycol and polylactic acid, formulated into solution A (the solution is brown); finally, the solution A is placed in a three-necked flask at 200 ° C for 20 min in a microwave reactor, and then rapidly added to the hot solution B, the reaction Instantly, at a constant temperature of 30 minutes.
- Constant temperature secondly, weighed 4.2g of polyphosphate with a weight average molecular weight of 30,000 Da in a beaker, weighed 300 ml of diethylene glycol in a beaker, stirred and sonicated to dissolve the two; quickly weighed anhydrous ferric chloride 2g It is dissolved in a mixture of diethylene glycol and polyphosphate by heating, sonicating, stirring to prepare solution A (the solution is brown); finally, solution A is placed in a three-necked flask at a constant temperature of 200 ° C for 20 min in a microwave reactor. Then, the hot solution B is quickly added, and the reaction is carried out instantaneously, and the temperature is kept for 30 minutes.
- each particle-modified polyacrylic acid contains 3339.60 carboxyl groups
- the free carboxyl group content of the polyacrylic acid-iron oxide complex molecule is 18.681 mmol per g Fe 3 O 4 , that is, it is required to chelate 11 carboxyl groups per 1.322 nm 2 , so the chelation density of the carboxyl group is 8 /nm 2 .
- the content of free carboxyl group in the molecule of iron oxide complex by conductometric titration is 18.681mmol per g Fe 3 O 4
- a particle-modified polyacrylic acid contains 3339.60 carboxyl groups, of which 2784 are free carboxyl groups, and about 80% are Sodium is saturated, so the number of molecules of Na is 2227 to 2784.
- the polyacrylic acid-iron oxide complex molecular solution obtained in Example 1-4 was dropped on the copper mesh of the carbon support film, and after being naturally air-dried, the morphology and size of the composite were observed under a transmission electron microscope (TEM).
- the original TEM image of the four batches of samples is shown in the left figure of Fig. 2.
- the particle size distribution of the composite molecular size in the figure is shown in the right figure of Fig. 2. It can be seen from Fig. 2 that the molecular weight of the composite molecule is good, and it is a single crystal nanoparticle molecule, and the interplanar spacing is 0.251 nm, corresponding to the crystal plane of triiron tetroxide (311). From the statistical particle size diagram, it is shown that the molecular size distribution of these nanocomposites is uniform, the crystallinity is high, and no agglomeration occurs between the particles.
- Example 1 the percentage of surface polyacrylic acid in the polyacrylic acid-iron oxide complex molecule in Example 1 is 27.5%, and the mass of acrylic acid in a polyacrylic acid-iron oxide complex is: 5.92 ⁇ 10 -19 g.
- the number of molecules of acrylic acid (C 3 H 4 O 2 ) contained was 5.92 ⁇ 10 -19 ⁇ 6.02 ⁇ 10 23 ⁇ 72 4950.
- the polyacrylic acid-iron oxide complex in Example 1 has an average molecular formula of Fe 12144 O 26092 C 14850 H 19800 ;
- Example 2 the average molecular formula of the polyacrylic acid-iron oxide complex in Example 2 was Fe 6960 O 39632 C 45528 H 60704 ;
- the polyacrylic acid-iron oxide complex in Example 3 has an average molecular formula of Fe 3339 O 19016 C 21846 H 29128 ;
- the polyacrylic acid-iron oxide complex of Example 4 had an average molecular formula of Fe 2064 O 10702 C 11925 H 15900 .
- Figure 12 shows a possible structural schematic of a polyacrylic acid-iron oxide complex molecule.
- the center is the grain of iron oxide nanoparticles, and the surface is a high-density polyacrylic acid polymer chain.
- Some carboxyl groups on the molecular chain are bonded to iron atoms in the iron oxide nanoparticles by chemical coordination bonds, and the remaining unchelated The carboxyl chain segment is extended and distributed in the particle The outer surface.
- the main feature of the molecular structure of the composite is that the density of iron atoms on the surface of the iron oxide is chelated by the carboxyl group to 2/square nanometer or more. This dense chelation makes the surface of the iron oxide have an ultra-high polyacrylic acid modification amount, which is reduced.
- the amount of modification also allows the outer surface of the composite to have a high density of extended carboxyl chains, imparting excellent hydrophilicity to the composite and a high negative potential in aqueous solution, greatly improving the dispersion stability of the particles.
- the particle size of the iron oxide nanoparticles in the composite is 1 to 10 nm under transmission electron microscopy, and the mass of the surface-coupled polymer polyacrylic acid accounts for 25% to 70% of the total mass of the composite molecules.
- the polyacrylic acid-iron oxide complex molecules of Examples 1-4 are in aqueous solution by surface zeta potential test.
- the surface potentials were: -41.3 mV, -42.8 mV, -45.1 mV, and -40.9 mV, respectively, indicating that the complex molecules carry a large amount of negative charge, and these excess negative charges allow the particles to be stably dispersed in the aqueous solution.
- the polyacrylic acid-iron oxide complex of Example 1-4 was dispersed in physiological saline, and as shown in the figure, the polyacrylic acid-iron oxide complex molecule was visually observed to be stably dispersed in physiological saline, and the solution color was uniform and absent.
- the sedimentation phenomenon indicates that the negative charge on the surface of the complex molecule enables the molecule to maintain good dispersibility in physiological saline.
- Example 1-4 Take about 5 mL of the polyacrylic acid-iron oxide complex molecular solution obtained in Example 1-4, freeze-dry, and weigh about 10-15 mg of solid powder before testing. The sample is wrapped in a weighing paper and wrapped into a flat rectangular shape. The vibrating sample magnetometer was tested at room temperature and the results are shown in Figure 3, where the inserted thumbnail is an enlarged view of the curve near zero.
- the saturation magnetizations of the polyacrylic acid-iron oxide complex molecules obtained in Examples 1-4 were: 62.6, 69.4, 49.6, and 49.1 emu/g, respectively. It can be seen by inserting a small figure that the magnetization curve passes through the origin, that is, there is no remanence, which proves that the novel polyacrylic acid-iron oxide complex molecules have superparamagnetism.
- the polyacrylic acid-iron oxide complex molecules obtained in Examples 1-4 were tested for Fe content, and then the samples were diluted to 4, 5, 6, 7, 8 ⁇ 10 -4 mol/L, and 200 uL of the diluted sample was added to the test.
- the relaxed tubes are numbered 4, 5, 6, 7, and 8, respectively.
- the tube was placed in a constant temperature water bath at 37 ° C. Open the test software and first calibrate and test the relaxation time of the sample.
- the relaxation time of the polyacrylic acid-iron oxide complex molecule in Examples 1-4 is shown in Figure 4.
- the r 1 relaxation rate is 5.61-17.5
- the r 2 relaxation rate is 20.3-72.7, where r 2 /r 1
- the values are between 3.2 and 4.2.
- the polyacrylic acid-iron oxide complex molecular solution prepared in any of the embodiments 1-4 is dried by freeze
- the polyacrylic acid-iron oxide complex molecules are obtained by drying or spray drying, and the content of iron elements therein is determined by atomic absorption spectrometry. According to the iron content obtained by the above measurement, a corresponding volume of water or physiological saline is added according to the final concentration of iron element (umol Fe/L).
- the final concentration of iron element is 5-1000 umol Fe/L, preferably 50-200 umol Fe/L.
- the above polyacrylic acid-iron oxide complex molecular solution which has been configured according to the final concentration requirement of the iron element is ultrasonically dispersed to prepare a stable polyacrylic acid-iron oxide complex molecular injection.
- the prepared polyacrylic acid-iron oxide complex injection was sterilized by autoclaving at 121 ° C for 30 minutes. After cooling, the stability of the injection was observed. The injection had no obvious sedimentation phenomenon, and the color of the solution did not change significantly.
- the sample was subjected to dynamic light scattering (DLS) to test the hydraulic preparation of the injection complex molecule after sterilization. As shown in Fig. 5, the particle size and particle size distribution of the polyacrylic acid-iron oxide complex before and after sterilization were not obvious. The change. It shows that the prepared polyacrylic acid-iron oxide complex injection can be sterilized by high temperature, which effectively improves the safety of the injection.
- DLS dynamic light scattering
- the polyacrylic acid-iron oxide complex molecular solution prepared by any one of the examples 1-4 is obtained by freeze drying or spray drying to obtain a polyacrylic acid-iron oxide complex molecule, and the content of iron element is determined by atomic absorption spectrometry. .
- the final concentration of iron element (umol Fe / L), the pharmaceutical auxiliary lactose (10% - 30%), starch (5% - 25%), ethyl cellulose (10) %-25%, dissolved in anhydrous ethanol), and anhydrous ethanol to make suitable wet granules, passed through 80 mesh stainless steel mesh, dried at room temperature, passed through 20 mesh stainless steel mesh, and added talcum powder (1%- 10%), stearic acid (0.2%-5%), evenly mixed, flat stamping.
- the final concentration of iron in the tablet is from 5 to 1000 umol Fe/kg, preferably from 50 to 200 umol Fe/kg.
- the polyacrylic acid-iron oxide complex molecular solution prepared by any one of the examples 1-4 is obtained by freeze drying or spray drying to obtain a polyacrylic acid-iron oxide complex molecule, and the content of iron element is determined by atomic absorption spectrometry. .
- the content of iron element is determined by atomic absorption spectrometry.
- the final concentration of iron umol Fe / L
- the final concentration of iron in the capsule is 5-1000 umol Fe/kg, preferably the final concentration of iron in the capsule is 50-200 umol Fe/kg.
- the polyacrylic acid-iron oxide complex molecular injection prepared in Example 16 was placed for three days to one year, and its stability was observed. The color of the injection solution did not change significantly, and the solution did not precipitate uniformly. Samples were taken to test the hydraulic diameter of the injection complex molecules by dynamic light scattering (DLS), as shown in Figure 6, with no significant changes. This indicates that the polyacrylic acid-iron oxide complex molecular injection agent has a high proportion of polyacrylic acid modification on its surface, and a high proportion of polyacrylic acid has excellent stability of the polyacrylic acid-iron oxide complex molecule due to its strong electrostatic repulsion force. Suitable for intravenous injection Injectable use.
- DLS dynamic light scattering
- the polyacrylic acid-iron oxide complex molecular injection obtained in Example 16 was used, and the supernatant was filtered using a 3KDa ultrafiltration centrifuge tube, and the free iron ions in the supernatant were determined by atomic absorption. concentration. A certain amount of free iron ions was also added to the injection, and after half an hour, the concentration of free iron ions in the supernatant was measured by atomic absorption, and the test results are shown in Table 1. The results showed that the injection did not release free iron ions even for 120 days. After the addition of free iron ions, the injection can simultaneously reduce the concentration of free iron ions, effectively reducing the hypersensitivity caused by imaging in vivo.
- the injection preparation of the novel iron oxide complex molecule can effectively reduce the release of free iron ions and reduce the hypersensitivity reaction, and is suitable for clinical use as a contrast agent and iron supplement.
- the polyacrylic acid-iron oxide complex molecular injection prepared in Example 16 was configured as a contrast agent having a concentration of 40, 85, and 135 umol Fe/L, and was injected into the model rabbit via the ear vein at a dose of 1 ml/kg, respectively, in the injection.
- T 1 weighted imaging of the rabbit liver was performed before, at 0 min, 3 min, 5 min, 10 min, 20 min, and 30 min after injection.
- the results of magnetic resonance imaging are shown in Figure 7: the liver signals of rabbits were decreased in different injection concentration models; the small hepatic vein branches in the liver of rabbits showed clear (40 arrow) in the 40umol/L injection concentration model; 85umol/L injection concentration model rabbits The trunk of the liver vein (shown by the arrow) in the liver showed clear, and the small branches were slightly blurred; the 135umol/L injection concentration model showed the main hepatic vein trunk and small branches in the liver of the rabbit, but the results were slightly lower than 85umol/L and 40umol/ respectively. L injection concentration model rabbit. This new type of iron oxide complex molecular injection can be used for magnetic resonance imaging of normal liver tissue.
- the aorta and vena cava were not ideal, the vascular signal was reduced, and the contour was blurred.
- the Fe contrast agent clearly showed the aorta, vena cava and portal vein for at least 30 min, and the effect was similar at each time point and prolonged with time.
- the finer spinal cord arteries and veins (shown by thick arrows) showed a slight increase in the range, while Gd-DTPA showed a small amount of spinal cord arteries only at 30 s. It shows that iron contrast agent can obtain vascular images with higher contrast, and has obvious long-circulation characteristics. After 30 minutes, it still has clear blood vessel imaging effect.
- the utility model overcomes the short development time of the commercial bismuth contrast agent, the unclear signal and the blurred outline. It is indicated that the novel iron oxide complex molecular contrast agent is capable of being applied to long-term vascular imaging in vivo due to its surface coupled with high-density polyacrylic acid, and the effect is far superior to the existing commercial sputum contrast agent.
- the tumor model rabbit liver was injected with the stabilized polyacrylic acid-iron oxide complex molecular injection prepared in Example 16 before and after (concentration: 135 umol/L; dose: 1 ml/kg), and respectively subjected to T 1 weighted imaging (T 1 WI). And T 2 weighted imaging (T 2 WI).
- T 1 WI and T 2 WI showed better results on tumors (shown by the arrow head) than before injection, and liver signals were significantly lower than before injection, and the lesions were prominent.
- the normal liver tissue with a significantly reduced signal after the contrast agent was injected showed a relatively high-signal tumor lesion, which made the contrast more intense.
- This new type of iron oxide complex molecular injection can be used for magnetic resonance imaging of diseased liver tissue (such as liver cancer), and both T 1 weighted imaging (T 1 WI) and T 2 weighted imaging (T 2 WI) can effectively Increasing the contrast between the diseased liver tissue and normal liver tissue is conducive to clinical diagnostic research and has a high potential for clinical application.
- the rabbit liver of the vascular aneurysm model was injected with the stabilized polyacrylic acid-iron oxide complex molecular injection prepared in Example 16 before and after (concentration: 135 umol/L; dose: 1 ml/kg).
- the results of magnetic resonance imaging are shown in Figure 10: a.
- Aneurysm model Rabbit left common carotid artery bridging aneurysm (shown in the head of the arrow) clearly shows the aortic model of the left common carotid artery in the left common carotid artery The diameter of the tumor is obviously larger than that of the common carotid artery, which is obviously different from the contralateral normal common carotid artery; b.
- the aneurysm is more obvious after removing the remaining blood vessels; c. and d.
- the lateral view of the aneurysm and the gross pathology of the aneurysm taken during the operation The form is highly consistent.
- This new type of iron oxide complex molecular injection can be used for magnetic resonance imaging of diseased blood vessels (such as aneurysms). Magnetic resonance imaging can effectively increase the contrast between diseased blood vessels and normal blood vessels.
- Clinical diagnostic research has the potential for extremely high clinical applications.
- Example 16 After microbes ( ⁇ 30 kg) were anesthetized with sodium pentobarbital, the polyacrylic acid-iron oxide complex molecular injection prepared in Example 16 (concentration: 135 umol/L; dose: 1 ml/kg) was intravenously administered to pigs. 3T GE Magnetic Resonance Imaging (Signa HDxt, 3T) imaging.
- Figure 11 The results of magnetic resonance imaging are shown in Figure 11:
- Figure a shows the imaging results of the 15-minute coronary artery (shown by the dotted line), and the coronary artery can be clearly displayed;
- b is the imaging result of the coronary artery (shown by the dotted line) after 180 minutes, and the coronary artery information Almost disappeared;
- c picture is the 15min coronary anterior descending artery (shown in dotted line) imaging results, the anterior descending coronary artery can be clearly displayed;
- d picture is the 15min coronary circumflex artery (shown in dotted line) imaging results, coronary circumflex Can be clearly displayed.
- This new type of iron oxide complex molecular injection can be used for magnetic resonance imaging of coronary arteries, which is beneficial to clinical diagnosis and has great potential for clinical application.
- Eighteen SD male rats about 200 g, were used and divided into three groups of 6 rats each.
- the first group normal control group: normal feeding; the second group (iron deficiency anemia control group): low-iron feeding; the third group (iron deficiency anemia treatment experimental group): low-iron feeding, blood sampling at week 4
- the polyacrylic acid-iron oxide complex injection prepared in Example 16 was injected afterwards; the food intake of each group was approximately the same during the experiment.
- Hgb Hemoglobin
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Abstract
Description
时间 | 加入自由铁离子量 | 下清自由铁离子含量 | |
1 | 120天 | —— | 0.24ug/mL |
2 | 2小时 | —— | 0.30ug/mL |
3 | 2小时 | 300ug/mL | 8.61ug/mL |
4 | 2小时 | 水 | 0.09ug/mL |
Claims (26)
- 一种高分子-金属氧化物复合物,其特征在于,所述高分子-金属氧化物复合物包括位于核心的金属氧化物颗粒和修饰在所述金属氧化物颗粒表面的高分子,所述高分子具有能与所述金属氧化物中的金属键合的官能团,所述高分子与所述金属氧化物颗粒表面结合位点的密度高于2个位点/平方纳米;所述高分子-金属氧化物复合物的平均分子通式为:MnNpOmCaHbNac,其中M代表金属元素,N为N、P或S,n为500-20000,p为0-20000,a为1000-50000,c为500-20000,m=(3/2~4/3)n+(2/3)a,b=(4/3)a。
- 根据权利要求1所述的高分子-金属氧化物复合物,其特征在于,所述高分子-金属氧化物复合物包含一种以上的金属元素。
- 根据权利要求1所述的高分子-金属氧化物复合物,其特征在于,所述高分子选自聚丙烯酸、聚丙烯酸盐、甲基聚丙烯酸、甲基聚丙烯酸盐、聚乳酸、聚乳酸盐和聚磷酸酯中的一种或几种。
- 根据权利要求1所述的高分子-金属氧化物复合物,其特征在于,所述高分子的重均分子量为500-500000Da。
- 根据权利要求4所述的高分子-金属氧化物复合物,其特征在于,所述高分子的重均分子量为500-3000Da。
- 根据权利要求1所述的高分子-金属氧化物复合物,其特征在于,所述高分子的总质量占所述高分子-金属氧化物复合物的25%-70%。
- 根据权利要求1所述的高分子-金属氧化物复合物,其特征在于,所述高分子的总质量占所述高分子-金属氧化物复合物的40%-70%。
- 根据权利要求1所述的高分子-金属氧化物复合物,其特征在于,所述金属氧化物选自氧化铁,氧化锰、氧化钴、氧化铬和氧化镍中的一种或几种。
- 根据权利要求1-8中任一项所述的高分子-金属氧化物复合物,其特征在于,所述金属氧化物为氧化铁。
- 根据权利要求1-8中任一项所述的高分子-金属氧化物复合物,其特征在于,所述高分子-金属氧化物复合物为聚丙烯酸-氧化铁复合物,所述聚丙烯酸与所述氧化铁颗粒表面结合位点的密度高于2个位点/平方纳米;所述聚丙烯酸-氧化铁复合物的平均分子通式为:FenOmCaHbNac,其中n为500-20000,a为1000-50000,c为500-20000,m=(3/2~4/3)n+(2/3)a,b=(4/3)a。
- 根据权利要求1-8中任一项所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述制备方法包括以下步骤:步骤1、将沉淀剂溶解在还原性溶剂中,配成溶液B;步骤2、将所述高分子溶解在所述还原性溶剂中;步骤3、称量金属盐,将所述金属盐溶解在步骤2中得到的混合溶液中,配成溶液 A;步骤4、将所述溶液A与所述溶液B在微波条件下进行反应;冷却,得到高分子-金属氧化物复合物分子胶体;步骤5、将步骤4中得到的所述高分子-金属氧化物复合物分子胶体分离洗涤除杂,得到所述高分子-金属氧化物复合物。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述还原性溶剂为亲水性高沸点溶剂,所述还原性溶剂的沸点在180℃以上。
- 根据权利要求11或12所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述还原性溶剂选自一缩二乙二醇、乙二醇、丙二醇、丙三醇、异丙二醇、二甘醇中的一种或几种。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述沉淀剂为氢氧化钠、醋酸钠或硼氢化钠。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述高分子选自聚丙烯酸、聚丙烯酸盐、甲基聚丙烯酸、甲基聚丙烯酸盐、聚乳酸、聚乳酸盐和聚磷酸酯中的一种或几种。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述金属盐选自三氯化铁、硫酸铁、氢氧化铁、乙酰丙酮铁、乙酰丙酮铁钴中的一种或几种。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,在所述微波条件下的反应温度为180℃-280℃。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,在所述微波条件下的反应时间为5min-30min。
- 根据权利要求11所述的高分子-金属氧化物复合物的制备方法,其特征在于,所述制备方法包括以下步骤:步骤1、将沉淀剂溶解在还原性溶剂中,配成溶液B;步骤2、将聚丙烯酸溶解在所述还原性溶剂中;步骤3、将铁盐溶解在步骤2中得到的混合溶液中,配成溶液A;步骤4、将所述溶液A与所述溶液B在微波条件下进行反应;冷却,得到聚丙烯酸-氧化铁复合物分子胶体;步骤5、将步骤4中得到的所述聚丙烯酸-氧化铁复合物分子胶体分离洗涤除杂,得到聚丙烯酸-氧化铁复合物。
- 一种核磁共振造影剂,其特征在于,所述核磁共振造影剂包含根据权利要求9或10所述的高分子-金属氧化物复合物。
- 根据权利要求20所述的核磁共振造影剂,其特征在于,所述核磁共振造影剂为 注射剂或口服制剂。
- 根据权利要求20或21所述的核磁共振造影剂在组织或细胞核磁显影中的应用。
- 根据权利要求22所述的应用,所述组织或细胞为血管、肝脏、脾脏、淋巴或心脏。
- 一种补铁剂,其特征在于,所述补铁剂包含根据权利要求9或10所述的高分子-金属氧化物复合物。
- 根据权利要求24所述的补铁剂,其特征在于,所述补铁剂为注射剂或口服制剂。
- 根据权利要求24或25所述的补铁剂在制备治疗缺铁性贫血药物中的应用。
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RU2812291C2 (ru) * | 2018-05-16 | 2024-01-29 | Сева Санте Анималь | Ветеринарные композиции и их применение для контролирования дефицита железа у млекопитающих, не являющихся человеком |
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Publication number | Priority date | Publication date | Assignee | Title |
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US20130251636A1 (en) * | 2010-12-02 | 2013-09-26 | Kyoto University | Mri contrast agent containing composite particles |
CN104069516A (zh) * | 2014-06-09 | 2014-10-01 | 上海交通大学 | 一种超顺磁性纳米颗粒及其制备方法和用途 |
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US20130251636A1 (en) * | 2010-12-02 | 2013-09-26 | Kyoto University | Mri contrast agent containing composite particles |
CN103208624A (zh) * | 2013-03-27 | 2013-07-17 | 东北师范大学 | 单分散核壳结构Fe3O4@C纳米复合锂电池负极材料的制备方法 |
CN104069516A (zh) * | 2014-06-09 | 2014-10-01 | 上海交通大学 | 一种超顺磁性纳米颗粒及其制备方法和用途 |
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WO2019219855A1 (en) * | 2018-05-16 | 2019-11-21 | Ceva Sante Animale | Veterinary compositions and the uses thereof for controlling iron deficiencies in non-human mammals |
RU2812291C2 (ru) * | 2018-05-16 | 2024-01-29 | Сева Санте Анималь | Ветеринарные композиции и их применение для контролирования дефицита железа у млекопитающих, не являющихся человеком |
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