WO2018016998A1

WO2018016998A1 - Biocidal composition and methods for the treatment of plants and animals using such a composition

Info

Publication number: WO2018016998A1
Application number: PCT/RU2017/050002
Authority: WO
Inventors: Вадим Альбертович КАСЬЯНЕНКО
Original assignee: Вадим Альбертович КАСЬЯНЕНКО
Priority date: 2016-07-22
Filing date: 2017-01-24
Publication date: 2018-01-25
Also published as: RU2629775C1

Abstract

The invention relates to biocidal compositions and to methods for using them in the treatment, prophylaxis and protection of plants and warm blooded animals (including humans) against fungal (mycotic) and bacterial diseases. The present biocidal composition contains ingredients in the following ratio (in wt%): 0.001-20 S-nonyl thiuronium bromide, and solvent as the remainder. A method for the biocidal treatment of plants involves bringing plant tissue or parts thereof into contact with the biocidal composition. A method for the biocidal treatment of animals involves applying said composition to external animal tissue. The technical result is that of improving the fungicidal and antibacterial properties of the composition, increasing the effectiveness and safety of the biocidal treatment of plant and animal tissues, and simplifying a method for the production and use of such a composition.

Description

Biocidal composition and methods for treating plants and animals with its use

Technical field

The group of inventions relates to biocidal compositions and methods of their use for the treatment, prevention and protection of plants and warm-blooded animals (including humans) from fungal (fungal) and bacterial diseases.

State of the art

Protection, treatment and prevention of plants and animals from bacterial and fungal diseases is an important and integral part of the technology of their cultivation, because it allows you to get the final product in the required quantity and quality, as well as to ensure profitability of production. One of the solutions to this problem is the use of biocidal compositions for fungicidal and antibacterial treatment of plant or animal tissues.

The prior art it is known the use as biocides of various salts of thiuronium, exhibiting antibacterial and fungicidal properties.

For example, the publication of international application WO0179359, 10.25.2001, discloses an anti-fouling agent containing an isothiuronium salt.

US Pat. No. 4,435,381, 03/06/1984 discloses the use of thiuronium phosphates for dental hygiene of animals, and also refers to the use of thiuronium bromides as fungicides for treating plants.

US Pat. No. 2,547,356, 04/03/1951, describes the use of phenyl groups containing thiuronium salts as antibacterial agents.

US patent US2980578, 04/18/1961 discloses a method for fungicidal treatment of plants using a solution of isothiuronium chlorides and bromides.

The closest analogue of the invention is a biocidal composition and method of its use, disclosed in British patent GB 1551224, 08/30/1979. The composition is an aqueous solution containing 0.001-10 wt.% S-substituted salt of thiuronium, as well as nonionic or cationic surfactants. As the thiuronium salt, a compound of the following formula is used:

where Ro is aralkyl, alkanoid, aralkanoyl, aroyl and allyl, and X is halogen

The specified analogue also disclosed a method of antibacterial treatment of human skin, including applying the specified composition.

However, the known composition has several disadvantages. The described salts of thiuronium halides in the radical group contain a benzene or phenolic ring.

When heated in the presence of halogens (such as chlorine or bromine) and atmospheric oxygen, cyclic aromatic compounds can form dioxin-like compounds and dioxin-like compounds that are dangerous to the health of living organisms (plants, animals, humans). Accordingly, there is a risk of the formation of these compounds during the synthesis of salts, which requires special control in the production and thorough purification of the substance.

Disclosure of invention

The objective of the claimed group of inventions is to create an effective, safe for plants and animals and easy to manufacture biocidal composition.

The technical result of the invention is to increase the fungicidal and antibacterial properties of the composition, increase the efficiency and safety of biocidal treatment for plant and animal tissues, and also simplify the method of its production and use.

The specified technical result is achieved in a biocidal composition for treating tissues of living organisms due to the fact that it contains a thiuronium salt and a solvent, while it contains s-nonyl thiuronium bromide in the following ratio of components, wt.%: S-nonyl thiuronium bromide 0.001-5, solvent - the rest.

Moreover, according to private options for the implementation of the composition:

- as a solvent used water or a solution of ethyl alcohol,

- the composition further comprises at least one component selected from the group comprising surfactants, compounds of sulfur, copper, iron, lime, compounds from the group of azoles, strobilurins, benzimidazoles, phenylamides, anilidopyrimidines, pyrimidinyl carbinols, dithianols, phosphanates, phthalamides, antibiotics, buffers, preservatives, antioxidants, wetting agents, antifoam agents, thickeners and humectants in an amount of 0.001-40 wt.%

The specified technical result is achieved in the method of biocidal treatment of plants due to the fact that it includes providing contact of the plant tissue or part thereof with said biocidal composition. In this case, the provision of contacting the plant with the composition can be realized by: spraying the composition of the plant surface, soaking the plant roots in the composition, applying the composition to the surface of the seeds, injecting the composition into the plant stem, applying the composition to the soil.

The specified technical result is achieved in the method of biocidal processing of animals, due to the fact that it includes the application to the external tissues of the animal of said biocidal composition

As the main active ingredient of the claimed composition used S-nonyl tiuronium bromide, which has the following advantages compared with known analogues.

1. Unlike the closest analogue, S-nonyl thiuronium bromide, as well as the compounds used for its synthesis, does not contain cyclic, aromatic radicals, which eliminates the risks of the formation of compounds dangerous for plants and animals from the group of dioxins and dioxin-like compounds.

2. Unlike the closest analogue, S-nonyl thiuronium bromide has not only bactericidal, but also fungicidal activity, which greatly expands the scope of its application. The substance has a bactericidal and fungicidal effect against a wide range of harmful bacteria and fungi - pathogens of plants and animals, such as bacteria from the genus Xanthomonas, which cause great damage to agricultural plants.

3. In contrast to the known analogues, S-nonyl thiuronium bromide has a significantly higher activity, since it ensures the death of harmful microorganisms in a concentration of from 0.0005 mg / ml.

4. S-nonyl thiuronium bromide has good solubility in water and other safe solvents. 5. S-nonyl thiuronium bromide has a low level of danger to plants and warm-blooded animals, including person. This, in turn, makes it possible to increase the safety level of the use of the claimed composition in biocidal treatment of plant or animal tissues at any concentration of the active component.

6. Substances of this class have not previously been used for the treatment of plants and animals, which determines not only the novelty of the invention, but also the effectiveness of the biocidal composition due to the presence of resistance of harmful bacteria and fungi to a number of drugs traditionally used to suppress and destroy them. This phenomenon occurs as a result of prolonged use of substances of the same chemical class. In this case, the natural selection and emergence of new strains of harmful microorganisms that are resistant to this class of substances occurs. As a result, protection, treatment, and prevention can and often are ineffective, yield, quality, loss of animal health or their loss occurs. The emergence of a substance from a new class with a new mechanism of action allows not only to destroy malicious, resistant to other previously used substances, but also to create a system of protection, treatment and prevention that prevents the emergence of new resistant strains.

S-nonyl thiuronium bromide has the chemical formula CioH ₂₃ N ₂ SBr and the structural formula:

The substance is a white crystal with a melting point of 98-100 ° C, well soluble in water. S-nonyl thiuronium bromide can be synthesized by heating a mixture of thiourea, nonyl bromide and ethanol.

The biocidal composition for treating plants and animals includes 0.001-20 wt.% S-nonyl thiuronium bromide and a solvent (the rest). When the contents of s-nonyl thiuronium bromide less than 0.001 wt.% the composition does not have the required efficiency, while the content of more than 20 wt.% is impractical for economic reasons (the most optimal content is up to 5 wt.%). The solvent used is preferably water. However, it is possible to use other solvents acceptable for contact with the tissues of plants and animals, for example, a solution of ethyl alcohol, etc.

The composition may also contain additional components, such as surfactants, sulfur compounds (ground and colloidal sulfur), copper (vitriol, copper chloroxide), iron (vitriol), lime, compounds from the group of azoles, strobilurins, benzimidazoles, phenylamides, anilidopyrimidines, pyrimidinyl carbinols, dithianols, phosphanates, phthalamides, antibiotics, buffers, preservatives, antioxidants, wetting agents, antifoam agents, thickeners, moisturizers. These auxiliary components can be introduced into the composition in the amount of 0.001-40 wt.%

A method for biocidal treatment of plants includes contacting the tissue of a part or the whole plant with said biocidal composition. The treatment can be carried out by spraying the surface of the plant, soaking the roots, applying seeds to the surface, injecting into the stem or applying the composition to the soil.

A method of biocidal treatment of animals involves applying the composition to their skin.

The concentration of s-nonyl thiuronium bromide for each case is selected individually depending on the purpose of treatment (treatment, prevention, protection, etc.) and the type of microorganism.

The following are specific examples of the use of the biocidal composition.

Example 1

In laboratory conditions, the fungicidal activity of the biocidal composition was analyzed with respect to a fungus of the genus Verticillium, a causative agent of diseases in cultivated and wild plants, such as vegetable crops: tomatoes, peppers and other nightshade, cotton, maple, and ornamental plants. Tables 1 and 2 show the toxicity test data of the composition when analyzing the conidial progeny of colonies grown on a medium with different concentrations of S-nonyl thiuronium bromide, as well as a semi-lethal dose (LD50)

Table 1. The toxicity of the biocidal composition for Verticillium. Age Concentration of the drug, mg / ml

colonies, 0.0005 0.001 0.005 0.02 0.01 0 days

Colony Diameter, mm

7 10 + 1 7 + 1 0 0 0 18 + 1

14 34 + 1 21 + 1 5 ± 1 0 0 56 + 1

21 47 + 1 30 + 1 7 + 1 0 0 74 + 1

28 69 + 1 45 + 1 10 + 1 0 0 84 + 1

Table 2. Comparative LD50 values for Verticillium claimed biocidal

compositions and known fungicides

The results show that the claimed composition has a strong fungicidal effect

Example 2

In the field, the fungicidal activity of the claimed composition was tested in relation to the fungus Peronoplasmopara (Pseudoperonospora) cubensis, the causative agent of the disease, powdery mildew in plants of the pumpkin family, including cucumber, squash, pumpkin and others. Spraying of cucumber plantings was carried out during the growing season with an aqueous solution containing 0.01 wt.% S-nonyl tiuronium bromide to protect against the above disease. Spraying was carried out on an area of 25 hectares with a field sprayer with a flow rate of 400 l / ha.

Table 3 presents the results on the effectiveness of the composition.

Table 3. The effectiveness of the claimed composition against downy mildew.

Accounting for the disease before Accounting for the disease in 7 Accounting for the disease in 14 days after treatment days after treatment

Distribution Degree Distribution Degree Distribution Degree,% lesion,% lesion,% nya,% ni,% lesion,

%

Control - 86 10 95 30 100 68 without

processing

Processing 86 10 50 12 14 3 biocidal

composition

th The results show that the claimed composition has a pronounced protective effect.

Example 3

In the field, the bactericidal activity of the composition against the bacterium Pseudomonas syringae pv was analyzed. lachrymans - causative agent of the disease angular leaf spot in a cucumber. Spraying of cucumber plantings was carried out during the growing season with an aqueous solution containing 0.01 wt.% S-nonyl tiuronium bromide to protect against the above disease. Spraying was carried out on an area of 18 hectares with a field sprayer with a flow rate of 400 l / ha.

Table 4 shows the results on the effectiveness of the composition

Table 4. Efficiency of S-nonyl thiuronium bromide against angular leaf spot.

The results show that the claimed composition has a pronounced protective bactericidal effect.

Example 4

The fungicidal activity of the claimed composition against trichophytosis in dogs was tested. Trichophytosis, also known as ringworm, is caused by the fungi Trichophyton, Microsporum and Epidermophyton and can affect domestic and farm animals, as well as humans. Before the use of the composition, the dog had severe symptoms of the disease - lesions of 15 - 20% of the body surface, manifested in the form of hair loss, redness and flaking of the skin. After applying the composition twice daily in the form of a 0.05% aqueous solution of S-nonyl tiuronium bromide by lubricating the affected areas and skin areas adjacent to them, after a week after the start of use, a sharp weakening of the symptoms of the disease was noted, and after two weeks their complete disappearance, which was expressed in the normalization of the color and condition of the skin and the beginning of the growth of hair in the affected areas.

Thus, the claimed composition has a high fungicidal and antibacterial activity and can be effectively and safely used to protect, prevent and treat plants and animals from fungal (fungal) and bacterial diseases.

Claims

Claim

1. Biocidal composition for treating tissues of living organisms, containing a thiuronium salt and a solvent, characterized in that as a thiuronium salt it contains s-nonyl thiuronium bromide in the following ratio, wt.%: S-nonyl thiuronium bromide 0.001-20,

solvent is the rest.

2. The composition according to claim 1, characterized in that the solvent used is water or a solution of ethyl alcohol.

3. The composition according to claim 1, characterized in that it further comprises at least one component selected from the group comprising surfactants, compounds of sulfur, copper, iron, lime, compounds from the group of azoles, strobilurins, benzimidazoles, phenylamides, anilidopyrimidines , pyrimidinylcarbinols, dithianols, phosphanates, phthalamides, antibiotics, buffers, preservatives, antioxidants, wetting agents, antifoam agents, thickeners and humectants, in an amount of 0.001-40 wt.%

4. A method for biocidal treatment of plants, comprising contacting plant tissue or part thereof with a biocidal composition according to any one of claims 1-3.

5. The method according to claim 4, in which the contacting of the plant with the composition is carried out by at least one method selected from the group comprising spraying the composition of the plant surface with the composition, soaking the plant roots in the composition, applying the composition to the seed surface, injecting the composition into the plant stem, application of the composition to the soil.

6. A method of biocidal treatment of animals, comprising applying to the external tissues of an animal a biocidal composition according to any one of paragraphs 1-3.