WO2018015419A1 - Pointe de pipette pour dispositif de pipetage automatique et procédé pour la réaliser - Google Patents

Pointe de pipette pour dispositif de pipetage automatique et procédé pour la réaliser Download PDF

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Publication number
WO2018015419A1
WO2018015419A1 PCT/EP2017/068200 EP2017068200W WO2018015419A1 WO 2018015419 A1 WO2018015419 A1 WO 2018015419A1 EP 2017068200 W EP2017068200 W EP 2017068200W WO 2018015419 A1 WO2018015419 A1 WO 2018015419A1
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WO
WIPO (PCT)
Prior art keywords
pipette tip
pipette
electrode
tip
tube
Prior art date
Application number
PCT/EP2017/068200
Other languages
German (de)
English (en)
Inventor
Philipp Ott
Original Assignee
Tecan Trading Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CH00950/16A external-priority patent/CH712735A1/de
Priority claimed from CH00523/17A external-priority patent/CH712764A2/de
Application filed by Tecan Trading Ag filed Critical Tecan Trading Ag
Priority to CN201780037833.7A priority Critical patent/CN109416369B/zh
Priority to EP17748670.1A priority patent/EP3452835B1/fr
Priority to US16/309,325 priority patent/US11298695B2/en
Publication of WO2018015419A1 publication Critical patent/WO2018015419A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
    • G01D5/00—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable
    • G01D5/12—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means
    • G01D5/14—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage
    • G01D5/24—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance
    • G01D5/241—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance by relative movement of capacitor electrodes
    • G01D5/2417—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance by relative movement of capacitor electrodes by varying separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
    • G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
    • G01F23/26—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields
    • G01F23/263—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00722—Communications; Identification
    • G01N35/00732—Identification of carriers, materials or components in automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/54—Labware with identification means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
    • G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
    • G01F23/26—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields
    • G01F23/263—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors
    • G01F23/268—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring variations of capacity or inductance of capacitors or inductors arising from the presence of liquid or fluent solid material in the electric or electromagnetic fields by measuring variations in capacitance of capacitors mounting arrangements of probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N2035/1025—Fluid level sensing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1011—Control of the position or alignment of the transfer device

Definitions

  • the present invention relates to the technical field of automated liquid processing systems, and more particularly relates to pipette tips, especially for single use (namely, disposable tips, abbreviated "DiTis”), which are for pipetting, i. for aspirating and / or dispensing or dispensing liquids. Furthermore, the present invention relates to pipetting devices with such
  • Pipette tips method for determining the volume of a sample liquid in such pipette tips, method for Recognition of such pipette tips on one
  • Pipetting device uses of such pipette tips and a set of such pipette tips.
  • Fluid handling systems designed to handle fluid volumes.
  • Liquid processing systems include in particular
  • pipette tips are designed for single use and are commonly referred to as “disposable pipette tips” or English as “disposable tips” (abbreviated to “DiTis”).
  • dispenser pipette tips or English as “disposable tips” (abbreviated to "DiTis”).
  • Pipetting device this is able to detect, if any, a pipette tip on the pipette tube
  • Pipetting device which can automatically detect different pipette tips by this method, proposed. There is therefore a need to provide suitable pipette tips, which in the said method for determining the volume of a sample liquid in such a pipette tip and for detecting such
  • Pipette tips can be used on a pipetting device.
  • Sample liquid in the pipette tip is enabled. This object is achieved according to the invention by the set in claim 1 pipette tip.
  • Pipette tip to provide at one end, with the help of a precise determination of the processed
  • Sample volumes or liquid volumes is made possible. This object is achieved according to the invention by the set in claim 13 pipetting device.
  • Pipetting device allows. This object is achieved according to the invention by the recognition method proposed in claim 18.
  • a pipette tip according to the invention is provided for connection to a pipette tube of a pipetting device and formed as an elongated tube which forms a pipette body having at its one, first end an opening for aspirating and / or dispensing liquids, and at its other end to ( fluid-tight) Connect to the pipette tube
  • volume measuring electrode of a measuring capacitor which further at least part of a in the pipette tip
  • absorbable sample liquid comprises as a counter electrode has.
  • the first electrode is located on an outer surface of the pipette body or is embedded in the pipette body.
  • the pipette body is conical or pyramidal.
  • the pipette body consists of an electric
  • non-conductive material in particular a non-conductive plastic, such as non-conductive polypropylene, which acts in particular at least as part of a dielectric of the measuring capacitor.
  • the first electrode consists of a conductive plastic, such as conductive polypropylene.
  • the electrode may also be made of metal, e.g. made of copper or silver.
  • the first electrode is strip-shaped and extends axially along the pipette tip, and in particular has a width in a range of 0.8 mm to 5 mm, preferably in the range of 0.8 mm to 2 mm, particularly preferably 1 mm, on.
  • the first electrode is strip-shaped, extends axially along the pipette tip and is
  • the first electrode does not extend to the opening at the first end and is in particular of this opening
  • the pipette body has a conductive at the first end
  • the conductive tip in particular a length in a range of 3 mm to 6 mm, preferably in the range of 4.5 mm to 5.5 mm, particularly preferably 5 mm, and more particularly, consists of the same material as the first electrode.
  • the first electrode does not extend to the conductive tip (or is not galvanically connected thereto) and in particular is spaced at least 0.3 mm from the conductive tip.
  • the pipette tip has a second electrode as
  • Immersion detection electrode which is arranged in particular substantially opposite to the first electrode, and which further consists in particular of the same material as the first electrode.
  • the second electrode is located on an inner and / or outer surface of the pipette body or forms a continuous part of a wall of the pipette
  • Pipette body whereby a direct contact with a surrounding sample liquid (i.e., within and / or outside the pipette tip) is made possible.
  • the second electrode is strip-shaped and extends axially along the tube, and in particular spans up to two-thirds of the circumference of the pipette tip, more particularly covers half of the circumference of the
  • the second electrode extends to the opening at the first end.
  • Pipette tip a disposable pipette tip, which
  • a pipetting device comprises a pipette tube, a pressure generating means and a (capacitance) measuring unit, wherein the pipette tube at its one, first end for fluid-tight connection of a proposed
  • Pipette tip is formed, and connected at its other end to the pressure generating means, wherein in a region for connecting the pipette tip a
  • Pipette tip which is connected to the (capacitance) measuring unit, whereby the (capacitance)
  • Measuring unit is designed to in response to a measured capacitance of a measuring capacitor, which by the first electrode and at least a portion of a recordable in the pipette tip sample liquid as
  • Counter electrode is formed to determine a volume of a sample liquid located in the pipette tip.
  • the (capacitance) measuring unit is further configured to use a conductive tip on the pipette tip and / or a second electrode on the pipette tip as
  • Immersion detection electrode Immerse immersion of the pipette tip in the sample liquid.
  • Pipetting device further comprises a detection unit for detecting whether the pipette tip at the first end of
  • Pipette tube is connected, and / or for detecting a characteristic feature of the pipette tip based on a measurement of a further capacitance, wherein the further capacity is dependent on one or more of the following characteristics:
  • a geometry of the pipette tip in particular a shape of the pipette tip, further in particular one
  • Pipette tip exists, more particularly one
  • Electrode and / or the conductive tip consists;
  • a geometry such as a length, width, thickness
  • Measuring a capacitance of a measuring capacitor comprising a first electrode at the pipette tip and a counter electrode formed by at least a portion of a sample liquid located in the pipette tip;
  • this includes
  • a method for detecting ( recognition method) a proposed pipette tip on a
  • Pipetting device with a pipette tube, which at its one, first end for releasably receiving a
  • Pipette tip is designed for aspirating or dispensing a liquid, and at its other end it is operatively connected to a pressure generating means, wherein the method comprises measuring a capacitance that is dependent on one or more of the following
  • Pipette tip exists, more particularly one
  • Electrode and / or the conductive tip consists; a geometry (such as a length, width, thickness
  • the manufacturing method further includes attaching a conductive tip to the first end of the tube and / or forming a second electrode on the pipette tip, particularly on an inner and / or outer surface of the tube or as a continuous portion of a wall of the tube This is done by means of the same multi-component injection molding process, and in particular simultaneously with the molding of the first electrode.
  • a use of the proposed pipette tip for determining a volume of a sample liquid, which is located in the pipette tip is provided by means of a first electrode as Volumenmesselektrode at the pipette tip.
  • a set of proposed pipette tips comprises at least two types of pipette tips, wherein the at least two types differ in that when
  • Pipette tips located in a first area and the
  • Capacitance of a second type of pipette tips is in a second region, wherein the first and second regions are non-overlapping, and wherein the at least two types differ in particular by one of the following features:
  • volume measuring electrode in a front view e) a schematic representation of a third embodiment of a pipette tip according to the invention with a coating over the volume measuring electrode in a cross-sectional view from above;
  • Fig. 2a is a schematic representation of a fourth
  • FIG. 3 is a schematic representation of a fifth
  • Fig. 4a is a schematic representation of a sixth
  • a dip detection electrode forming a continuous part of the wall of the pipette body in a cross-sectional view from above;
  • Fig. 5a is a schematic representation of a dip
  • 6a a schematic representation of an aspiration and dispensing process of the sixth embodiment of a pipette tip according to the invention in the container with the
  • Fig. 7 is a schematic representation of a
  • Fig. 8 is a schematic representation of three
  • Fig. La is a schematic representation of a first embodiment of a pipette tip 1 with a strip-shaped Volumenmesselektrode 7 shown in a view from the side.
  • the elongate tube forming the pipette body 4 has a substantially conical shape.
  • the pipette body 4 could also be pyramidal, for example.
  • the pipette body 4 At its one, first end, the pipette body 4 has an opening 5 for aspirating and / or dispensing liquids.
  • the other End is formed for fluid-tight connection to the pipette tube 2, wherein the pipette tube 2 thereto
  • Volumenmesselektrode 7 acts as a first electrode of a measuring capacitor, which further comprises at least a portion of a recordable in the pipette tip 1 sample liquid as a counter electrode.
  • the pipette body 4 forms the dielectric of this measuring capacitor.
  • Volume measuring electrode 7 is arranged here as a narrow strip on the outer surface of the pipette body 4 and extends axially along the pipette tip 1 (with central axis a).
  • the volume measuring electrode 7 has e.g. a width in a range of 0.8 mm to 5 mm, preferably in the range of 0.8 mm to 2 mm, particularly preferably 1 mm.
  • the volume measuring electrode 7 has e.g. a width in a range of 0.8 mm to 5 mm, preferably in the range of 0.8 mm to 2 mm, particularly preferably 1 mm.
  • FIG. 1b) shows this first exemplary embodiment of the pipette tip 1 in a cross-sectional view from above, and in FIG. 1c) in a view from the front onto the volume measuring electrode 7.
  • FIG. 1 d a second exemplary embodiment of a pipette tip 1 with a staircase-shaped volume measuring electrode 7 in a view from the front is shown in a schematic representation.
  • capacitive jumps in the volume characteristic, i.e. the volume of the volume
  • volume measuring electrode 7 exceeds.
  • the level or the volume of the sample liquid in the pipette tip 1 largely independent of the
  • Fig. Le shows a third embodiment of
  • coating 8 may be present only in a region of the volume sensing electrode 7, e.g. in order to
  • Pipette tip 1 is lowered too far into the sample liquid.
  • the entire pipette tip 1 may be coated with a coating 8, e.g. with a hydrophobic, oleophobic or antibacterial coating 8.
  • Fig. 2a is a schematic representation of a fourth embodiment of a pipette tip 1 with an embedded in the pipette body 4
  • volume measuring electrode 7 shown in a view from the side.
  • the volume electrode 7 can be almost up to this extend to the opening 5.
  • the same pipette tip 1 is shown in Fig. 2b) in a view from above.
  • the volume measuring electrode 7 is enveloped on all sides by the material of the pipette body 4 and enclosed therein.
  • FIG. 3 shows a schematic representation of a fifth embodiment of a pipette tip 1 in a view from the side, which, in contrast to the first embodiment, has a conductive tip 9 with the opening 5 for aspirating and dispensing the sample liquid.
  • This conductive tip serves as
  • Immersion detection electrode (or contact), with the help of which it can be determined when the pipette tip 1 touches the surface of the sample liquid or
  • the conductive tip 9 has a length in a range of 3 mm to 6 mm, preferably in the range of 4.5 mm to 5.5 mm, particularly preferably 5 mm, and consists e.g. from the same material as the volume measuring electrode 7.
  • FIG. 4 a shows a schematic representation of a sixth exemplary embodiment of a pipette tip 1 which, in addition to the volume measuring electrode 7, has a second electrode as the immersion detection electrode (or contact) 10
  • this immersion detection electrode 10 can be (as with the conductive tip 9) to determine when the pipette tip 1, the surface of the
  • Immersion detection electrode 10 may be strip-shaped and run axially along the pipette body 4 as well as e.g. spanning half of the circumference of the pipette tip 1. Next extends the
  • volume measuring electrode 7 does not come into contact with the sample liquid, so that this e.g. not until the
  • Opening 5 extends and is spaced therefrom, as shown in Fig. 4a).
  • FIG. 5a illustrates in a schematic representation a use of the pipette tip 1 according to the sixth embodiment for immersion detection (-> cLLD), ie for the determination of the time when the pipette tip 1 into a sample liquid 6, which is in a container 19, such as a tub, a sample tube or a
  • Immersion detection electrode 10 is pulled out of the sample liquid 6.
  • Fig. 6a illustrates the use of the pipette tip 1 according to the sixth embodiment for measuring the volume of the sample liquid 6, which in the
  • Pipette tip 1 is located during aspiration and dispensing. As can be seen from the time course of the measured capacitance in FIG. 6b), the capacitance increases abruptly as soon as the immersion detection electrode 10
  • FIG. 7 illustrates in a simplified schematic representation an embodiment of a Pipetting device according to the invention. This illustration shows the pipette tube 2 with an attached
  • Pipette tip 1 which was previously removed from a receptacle 16 of a pipette tip holder 15 and now moved by a robot arm 14 on a liquid processing system 3 vertically upward and thus of the
  • Pipette tip holder 15 is removed.
  • a capacitance measuring unit 11 which with the pipette tube 2 and thus also with the pipette tip 1 as a first electrode and the work table or the work surface 17 and thus also with the pipette tip holder 15 (or a component thereof) as a second (counter) electrode connected, determine the (absolute) capacity of the measuring capacitor comprising these two electrodes.
  • the type or type e.g., size / volume
  • the measured value of the capacitance will be different.
  • Detection unit 12 compared to various reference values, each reference value is characteristic of a particular type or type of
  • the detection unit 12 then gives information about whether a pipette tip 1 at the
  • Pipette tube 2 is connected, and if so what type or type of pipette tip 1 it is, to a control unit 13 of a drive for the robot arm 14 on.
  • Such a Pipettenspit zenhalterung 15 may for example be part of the liquid processing system 3, wherein the robot arm 14 for determining the capacity and thus for detecting the pipette tip type or the
  • the pipette tip holder 15 is a separate / dedicated "measuring station" for Pipettenspit zenerkennung within the liquid processing system 3.
  • FIGS. 8a) -c) show, by way of example, three pipette tips 1 with electrodes 7 (or 10) of different widths, which comprise a set of three distinguishable types
  • volume measuring electrode 8 electrically insulating layer / coating (above the first electrode and the pipette body)
  • Control unit for controlling the movement of the drive of the pipetting robot
  • 19 container eg a microplate with wells a axis of the pipette tip b a electrode width from electrode width bb c electrode width cx first horizontal axis of movement of the pipetting robot (eg forward and backward) y second horizontal axis of movement of the pipetting robot
  • Pipetting robot for example to the left and right
  • z vertical movement axis of the pipetting robot down and up

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Power Engineering (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Thermal Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Fluid Mechanics (AREA)
  • Electromagnetism (AREA)
  • Robotics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne des pointes de pipette (1) destinées à être reliées à un tube de pipette (2) d'un dispositif de pipetage qui sert à prélever et à émettre des liquides. La pointe de pipette (1) selon l'invention prend la forme d'un tube allongé qui forme un corps de pipette (4) qui présente à l'une de ses extrémités une ouverture (5) et est conçu à son autre extrémité pour être raccordé au tube de pipette (2), la pointe de pipette (2) étant caractérisée en ce qu'elle présente une électrode (7) en tant qu'électrode de mesure de volume d'un condensateur de mesure. L'invention concerne par ailleurs des dispositifs de pipetage dotés d'une pointe de pipette (1) de ce type, des procédés pour déterminer le volume d'un échantillon de liquide dans une pointe de pipette (1) de ce type, des procédés pour reconnaître une pointe de pipette (1) sur un dispositif de pipetage, des procédés pour réaliser une pointe de pipette (1) de ce type, des utilisations d'une pointe de pipette (1) de ce type et un ensemble de pointes de pipette (1) de ce type.
PCT/EP2017/068200 2016-07-22 2017-07-19 Pointe de pipette pour dispositif de pipetage automatique et procédé pour la réaliser WO2018015419A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201780037833.7A CN109416369B (zh) 2016-07-22 2017-07-19 用于自动化移液设备的移液器吸头及其制造方法
EP17748670.1A EP3452835B1 (fr) 2016-07-22 2017-07-19 Pointe de pipette pour dispositif de pipetage automatique
US16/309,325 US11298695B2 (en) 2016-07-22 2017-07-19 Pipette tip for an automated pipetting device and production method thereof

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CH00950/16A CH712735A1 (de) 2016-07-22 2016-07-22 Pipettiervorrichtung mit einem Flüssigkeitsvolumensensor und Flüssigkeitsbearbeitungssystem.
CH00950/16 2016-07-22
CH00159/17A CH712761A2 (de) 2016-07-22 2017-02-10 Pipettiervorrichtung mit einer Pipettenspitzenerkennungseinheit und Verfahren zum Erkennen einer Pipettenspitze an einer Pipettiervorrichtung.
CH00159/17 2017-02-10
CH00523/17 2017-04-19
CH00523/17A CH712764A2 (de) 2016-07-22 2017-04-19 Pipettenspitze aufweisend eine Volumenmesselektrode sowie Verfahren zu deren Herstellung und Pipettiervorrichtung.

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WO2018015419A1 true WO2018015419A1 (fr) 2018-01-25

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PCT/EP2017/068200 WO2018015419A1 (fr) 2016-07-22 2017-07-19 Pointe de pipette pour dispositif de pipetage automatique et procédé pour la réaliser
PCT/EP2017/068497 WO2018015545A1 (fr) 2016-07-22 2017-07-21 Procédé pour déterminer la position d'un bras robotisé dans un système de manipulation de liquide et système de manipulation de liquide correspondant
PCT/EP2017/068495 WO2018015544A1 (fr) 2016-07-22 2017-07-21 Dispositif de pipettage, système de traitement de liquide et procédé de fonctionnement d'un système de traitement de liquide
PCT/EP2017/068494 WO2018015543A1 (fr) 2016-07-22 2017-07-21 Pointe de pipette destinée à un dispositif de pipetage automatique

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PCT/EP2017/068497 WO2018015545A1 (fr) 2016-07-22 2017-07-21 Procédé pour déterminer la position d'un bras robotisé dans un système de manipulation de liquide et système de manipulation de liquide correspondant
PCT/EP2017/068495 WO2018015544A1 (fr) 2016-07-22 2017-07-21 Dispositif de pipettage, système de traitement de liquide et procédé de fonctionnement d'un système de traitement de liquide
PCT/EP2017/068494 WO2018015543A1 (fr) 2016-07-22 2017-07-21 Pointe de pipette destinée à un dispositif de pipetage automatique

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WO2021018977A1 (fr) 2019-07-31 2021-02-04 Tecan Trading Ag Dispositif de déplacement et procédé de déplacement de volumes de fluide

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