WO2018005391A1 - Systèmes à haute résolution, kits, appareil et procédés utilisant des billes magnétiques pour des applications de microbiologie à haut débit - Google Patents

Systèmes à haute résolution, kits, appareil et procédés utilisant des billes magnétiques pour des applications de microbiologie à haut débit Download PDF

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Publication number
WO2018005391A1
WO2018005391A1 PCT/US2017/039346 US2017039346W WO2018005391A1 WO 2018005391 A1 WO2018005391 A1 WO 2018005391A1 US 2017039346 W US2017039346 W US 2017039346W WO 2018005391 A1 WO2018005391 A1 WO 2018005391A1
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WO
WIPO (PCT)
Prior art keywords
microwells
microwell
array
microfabricated device
chip
Prior art date
Application number
PCT/US2017/039346
Other languages
English (en)
Inventor
Alexander Hallock
Michael Sha
Original Assignee
General Automation Lab Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Automation Lab Technologies, Inc. filed Critical General Automation Lab Technologies, Inc.
Priority to JP2018567787A priority Critical patent/JP2019520825A/ja
Priority to CN201780040319.9A priority patent/CN110049817B/zh
Priority to SG11201811781XA priority patent/SG11201811781XA/en
Priority to EP17821035.7A priority patent/EP3478416A4/fr
Priority to AU2017289055A priority patent/AU2017289055A1/en
Priority to CA3027166A priority patent/CA3027166A1/fr
Publication of WO2018005391A1 publication Critical patent/WO2018005391A1/fr
Priority to IL263869A priority patent/IL263869A/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/04—Magnetic separation acting directly on the substance being separated with the material carriers in the form of trays or with tables
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/32—Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0335—Component parts; Auxiliary operations characterised by the magnetic circuit using coils
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/30—Combinations with other devices, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Abstract

L'invention concerne un procédé de transfert de matière d'un premier dispositif microfabriqué à un second dispositif microfabriqué. Au moins une bille magnétique est chargée dans au moins un micropuits du premier dispositif microfabriqué, où une pluralité de cellules sont cultivées. Le second dispositif microfabriqué est positionné de telle sorte que le ou les micropuits d'un premier réseau de micropuits soient alignés sur au moins un micropuits d'un second réseau de micropuits. Un champ magnétique est appliqué de façon à déplacer la ou les billes magnétiques contenues dans le ou les micropuits du premier dispositif microfabriqué jusque dans le ou les micropuits du second dispositif microfabriqué. De cette manière, au moins une cellule de la pluralité de cellules dans le ou les micropuits du premier dispositif microfabriqué est transférée au(x) micropuits du second dispositif microfabriqué.
PCT/US2017/039346 2016-06-30 2017-06-27 Systèmes à haute résolution, kits, appareil et procédés utilisant des billes magnétiques pour des applications de microbiologie à haut débit WO2018005391A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2018567787A JP2019520825A (ja) 2016-06-30 2017-06-27 磁性粒子を用いたハイスループット微生物学適用高分解能システム、キット、装置および方法
CN201780040319.9A CN110049817B (zh) 2016-06-30 2017-06-27 将材料从第一微加工装置转移到第二微加工装置的方法、和用于其的试剂盒
SG11201811781XA SG11201811781XA (en) 2016-06-30 2017-06-27 High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
EP17821035.7A EP3478416A4 (fr) 2016-06-30 2017-06-27 Systèmes à haute résolution, kits, appareil et procédés utilisant des billes magnétiques pour des applications de microbiologie à haut débit
AU2017289055A AU2017289055A1 (en) 2016-06-30 2017-06-27 High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
CA3027166A CA3027166A1 (fr) 2016-06-30 2017-06-27 Systemes a haute resolution, kits, appareil et procedes utilisant des billes magnetiques pour des applications de microbiologie a haut debit
IL263869A IL263869A (en) 2016-06-30 2018-12-20 High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662357142P 2016-06-30 2016-06-30
US62/357,142 2016-06-30

Publications (1)

Publication Number Publication Date
WO2018005391A1 true WO2018005391A1 (fr) 2018-01-04

Family

ID=60785484

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/039346 WO2018005391A1 (fr) 2016-06-30 2017-06-27 Systèmes à haute résolution, kits, appareil et procédés utilisant des billes magnétiques pour des applications de microbiologie à haut débit

Country Status (8)

Country Link
EP (1) EP3478416A4 (fr)
JP (1) JP2019520825A (fr)
CN (1) CN110049817B (fr)
AU (1) AU2017289055A1 (fr)
CA (1) CA3027166A1 (fr)
IL (1) IL263869A (fr)
SG (1) SG11201811781XA (fr)
WO (1) WO2018005391A1 (fr)

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CN109331996A (zh) * 2018-11-06 2019-02-15 中南大学 一种单面齿板介质、双面齿板介质、齿板介质盒以及平环强磁选机
WO2021077057A1 (fr) * 2019-10-18 2021-04-22 General Automation Lab Technologies Inc. Utilisation de la résorufine pour surveiller l'activité métabolique de cellules en conditions anaérobies
US20210252500A1 (en) * 2020-02-18 2021-08-19 General Automation Lab Technologies Inc. Method of Transferring Material from Microfabricated Device
CN109331996B (zh) * 2018-11-06 2024-05-14 中南大学 一种单面齿板介质、双面齿板介质、齿板介质盒以及平环强磁选机

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WO2016118915A1 (fr) * 2015-01-22 2016-07-28 Becton, Dickinson And Company Dispositifs et systèmes permettant le codage moléculaire à barres d'acides nucléiques cibles dans des cellules individuelles

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109331996A (zh) * 2018-11-06 2019-02-15 中南大学 一种单面齿板介质、双面齿板介质、齿板介质盒以及平环强磁选机
CN109331996B (zh) * 2018-11-06 2024-05-14 中南大学 一种单面齿板介质、双面齿板介质、齿板介质盒以及平环强磁选机
WO2021077057A1 (fr) * 2019-10-18 2021-04-22 General Automation Lab Technologies Inc. Utilisation de la résorufine pour surveiller l'activité métabolique de cellules en conditions anaérobies
US20210252500A1 (en) * 2020-02-18 2021-08-19 General Automation Lab Technologies Inc. Method of Transferring Material from Microfabricated Device
US11684914B2 (en) * 2020-02-18 2023-06-27 Isolation Bio Inc. Method of transferring material from microfabricated device

Also Published As

Publication number Publication date
EP3478416A4 (fr) 2019-12-18
CN110049817A (zh) 2019-07-23
AU2017289055A9 (en) 2021-09-23
AU2017289055A1 (en) 2018-12-20
IL263869A (en) 2019-01-31
JP2019520825A (ja) 2019-07-25
EP3478416A1 (fr) 2019-05-08
CN110049817B (zh) 2022-04-15
CA3027166A1 (fr) 2018-01-04
SG11201811781XA (en) 2019-01-30

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