CA3037631A1 - Systemes, kits, appareil et procedes a haute resolution pour determination de relation communautaire bacterienne et autres applications de microbiologie a haut debit - Google Patents

Systemes, kits, appareil et procedes a haute resolution pour determination de relation communautaire bacterienne et autres applications de microbiologie a haut debit Download PDF

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Publication number
CA3037631A1
CA3037631A1 CA3037631A CA3037631A CA3037631A1 CA 3037631 A1 CA3037631 A1 CA 3037631A1 CA 3037631 A CA3037631 A CA 3037631A CA 3037631 A CA3037631 A CA 3037631A CA 3037631 A1 CA3037631 A1 CA 3037631A1
Authority
CA
Canada
Prior art keywords
microwells
cells
microwell
sample
microfabricated device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3037631A
Other languages
English (en)
Inventor
Peter CHRISTEY
Alexander Hallock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Isolation Bio Inc
Original Assignee
General Automation Lab Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Automation Lab Technologies Inc filed Critical General Automation Lab Technologies Inc
Publication of CA3037631A1 publication Critical patent/CA3037631A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0244—Drop counters; Drop formers using pins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/54—Labware with identification means
    • B01L3/545—Labware with identification means for laboratory containers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00—Methods specially adapted for identifying library members
    • C40B20/02—Identifying library members by their fixed physical location on a support or substrate

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'analyse d'un échantillon comprenant une population d'entités biologiques à l'aide d'au moins un dispositif microfabriqué. Une pluralité des micropuits sur le dispositif microfabriqué sont chacun indexés de manière unique, et chargés avec un échantillon de telle sorte qu'au moins certains micropuits comprennent chacun plus d'une cellule d'une entité biologique. Le dispositif microfabriqué a été incubé à des conditions prédéterminées, et un matériel génétique sélectionné des cellules des entités biologiques obtenues à partir de l'incubation est amplifié pour obtenir des amplicons. Un agrégat des amplicons est séquencé pour obtenir des données de séquençage, sur la base desquelles et l'indexation des micropuits, une identification des entités biologiques présentes dans chacun de la pluralité de micropuits est obtenue. Une telle identification peut ensuite être utilisée pour déterminer une relation entre différents types d'entités biologiques dans l'échantillon.
CA3037631A 2016-09-28 2017-09-28 Systemes, kits, appareil et procedes a haute resolution pour determination de relation communautaire bacterienne et autres applications de microbiologie a haut debit Abandoned CA3037631A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662400841P 2016-09-28 2016-09-28
US62/400,841 2016-09-28
PCT/US2017/054108 WO2018064385A1 (fr) 2016-09-28 2017-09-28 Systèmes, kits, appareil et procédés à haute résolution pour détermination de relation communautaire bactérienne et autres applications de microbiologie à haut débit

Publications (1)

Publication Number Publication Date
CA3037631A1 true CA3037631A1 (fr) 2018-04-05

Family

ID=61760120

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3037631A Abandoned CA3037631A1 (fr) 2016-09-28 2017-09-28 Systemes, kits, appareil et procedes a haute resolution pour determination de relation communautaire bacterienne et autres applications de microbiologie a haut debit

Country Status (7)

Country Link
EP (1) EP3519096A4 (fr)
JP (1) JP2019531730A (fr)
CN (1) CN109843435A (fr)
AU (1) AU2017335897A1 (fr)
CA (1) CA3037631A1 (fr)
IL (1) IL265540A (fr)
WO (1) WO2018064385A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111483096B (zh) * 2019-12-10 2022-04-08 中国科学院亚热带农业生态研究所 根际通道微流控芯片及其胶体块和一种制造胶体块的模具
CN115867676A (zh) * 2020-05-29 2023-03-28 国立研究开发法人理化学研究所 细胞群的处理方法和细胞群中所含的基因的分析方法
EP3939699A1 (fr) * 2020-07-17 2022-01-19 Biomillenia SAS Modulateur de croissance
WO2024135694A1 (fr) * 2022-12-23 2024-06-27 ペプチドリーム株式会社 Procédé d'amplification d'adn à l'aide d'une pcr

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69733366T2 (de) * 1997-04-09 2006-01-26 Minnesota Mining And Mfg. Co., St. Paul Verfahren und vorrichtungen zur unterteilung biologischer probenflüssigkeiten in mikroflüssigkeitsmengen
US20050070005A1 (en) * 1997-06-16 2005-03-31 Martin Keller High throughput or capillary-based screening for a bioactivity or biomolecule
WO2003044484A2 (fr) * 2001-11-16 2003-05-30 Sloan-Kettering Institute For Cancer Research Microreseaux cellulaires haute densite pour determinations fonctionnelles paralleles
CN1313622C (zh) * 2002-08-02 2007-05-02 赵翀 高通量细胞生物芯片检测技术及试剂盒
CN1444043A (zh) * 2002-12-25 2003-09-24 帕弗瑞生物技术(北京)有限公司 一种个体化特异性免疫细胞功能测定方法
JPWO2005090549A1 (ja) * 2004-03-19 2008-02-07 ジェノミディア株式会社 細胞培養用マイクロウェル
CN1920559A (zh) * 2005-08-24 2007-02-28 赵翀 一种细胞生物技术、试剂盒及制备装置
AU2007233621A1 (en) * 2006-03-31 2007-10-11 Licentia Oy Method and microarray for detecting herpesviruses
US9850482B2 (en) * 2013-03-07 2017-12-26 Seoul National University R&Db Foundation Heterologous DNA barcoding method
EP2805769A1 (fr) * 2013-05-24 2014-11-26 European Molecular Biology Laboratory Procédés d'analyse de cellule unique à l'échelle nanométrique
MX361481B (es) * 2013-06-27 2018-12-06 10X Genomics Inc Composiciones y metodos para procesamiento de muestras.
WO2015013214A2 (fr) * 2013-07-21 2015-01-29 Whole Biome, Inc. Procédés et systèmes de caractérisation, de surveillance et de traitement du microbiome
KR102536833B1 (ko) * 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
EP3097211B1 (fr) * 2014-01-25 2023-09-13 Psomagen, Inc. Procédé et système d'analyse du microbiome
US20190102512A1 (en) * 2014-11-17 2019-04-04 Yeda Research And Development Co., Ltd. Method of analyzing microbiome
ES2836802T3 (es) * 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP3285926B1 (fr) * 2015-04-21 2022-03-02 General Automation Lab Technologies Inc. Trousse et procédure pour applications de microbiologie à rendement élevé

Also Published As

Publication number Publication date
EP3519096A1 (fr) 2019-08-07
JP2019531730A (ja) 2019-11-07
EP3519096A4 (fr) 2020-06-17
IL265540A (en) 2019-05-30
WO2018064385A1 (fr) 2018-04-05
CN109843435A (zh) 2019-06-04
AU2017335897A1 (en) 2019-04-04

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20240109