WO2017204412A1 - Disposable absorbent product comprising deodorizing composition having anti-bacterial and anti-inflammatory activity - Google Patents

Abstract

The present invention relates to a composition helpful in the inhibition and elimination of odor causing materials by: maximizing a deodorizing effect by fermenting Ecklonia cava and increasing the amount of active ingredients; having effects of alleviating and preventing oral diseases and halitosis, which is caused by microorganisms in the oral cavity and the like, by containing active ingredients of a Chinese plum extract and a Jerusalem artichoke extract; and having an effect and the like of alleviating constipation and odor of excreta through active bowel movements. According to the present invention, the results of a cytotoxicity and single-dose toxicity test show nearly no toxicity, and the composition has excellent deodorizing, anti-bacterial, anti-inflammatory and anti-oxidant effects, thereby being applicable and prepared as a deodorizing composition and a disposable absorbent product comprising the same.

Description

Disposable absorbent product containing deodorant composition with antibacterial and anti-inflammatory activity

The present invention relates to a disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, in particular, extracts of natural plants, that is, fermented Ecklonia cava extract as an active ingredient, and furthermore effective in extracting plum and pig potato extract A further absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity against Streptococcus aureus and Escherichia coli as an excellent deodorant effect on the odor derived from the human body, including deodorant, in particular, as an ingredient will be.

Many kinds of odors, including bad breath and bad smell, occur in the human body.

Bad breath refers to bad breath or bad breath by incorporating unpleasant odors coming from the oral cavity out of odors coming from systemic organs other than the oral cavity, such as the stomach, liver and lungs, as well as bad odors caused by the oral cavity of the human body. As described above, bad breath consists of volatile sulfur compounds generated by enzymes in the oral cavity decomposed by epithelial connective tissue, plant residue, and bacteria in the oral cavity as protein sources. Among them, methyl mercaptan, hydrogen sulfide, dimethyl sulfide, and the like, in particular, methyl mercaptan is known as the most disgusting smell during bad breath.

Depending on the cause of bad breath, oral causes and oral causes are divided into 85 or 90% of the oral causes. Bad breath caused by oral causes may result from bacterial decay of host factors such as teeth and saliva components and food residues, including salivary secretion, stomatitis, periodontal disease, inadequate prosthesis, and tongue areas. Excessive microbial deposition and the like, smoking, drinking and other saliva decrease, salivary viscosity increase, oral microorganisms, such as caries and periodontal disease is the biggest cause.

It is known that about 300 kinds of microorganisms reside in the surface of the tooth, between the teeth and gums of the root, the surface of the tongue, and the presence of such microorganisms is normal when proper oral hygiene activity is performed. However, pathogenic microorganisms can cause oral diseases such as caries, gingivitis or periodontal disease. Streptococcus mutans in the oral cavity resident (Streptococucus oral microorganisms such as mutans ) and Streptococcus sobrinus adhere to the surface of the tooth, and after a few hours, they form a bacterial community called plaque and multiply. Formation of plaque, but gradually progression will form plaque on the tooth surface in the subgingival area. When plaque is formed on the tooth surface, different microorganisms produce acid by using sugars in the oral cavity, and the acid decays the enamel of the tooth, causing tooth decay. On the other hand, among the bacteria in the subgingival plaque, especially anaerobic oral microorganisms secrete toxins and proteolytic enzymes, which directly destroy the periodontal tissue or react with the immune cells of the body to induce the production of various immune substances, and these substances are periodontal May cause inflammation and destruction of tissue. When caries and periodontal disease occur through this process, food debris is attached to this area to worsen bad breath.

Therefore, it is very urgent to develop a component having a dual effect by eliminating the root cause by preventing tooth decay and periodontal disease that causes bad breath.

The market size of related products continues to grow as more and more consumers use 'oral cleaning agents' that can't be brushed easily or are easy to carry for cooling the mouth. Recently, as various products with enhanced efficacy and effects such as tooth decay, gum disease prevention, and plaque removal have been released, consumers' interest in related products is also increasing. However, most of the oral antibacterial agents include chlorohexidin (chlorohexidin) and cetylpyridinium chloride (Cetyl Pyridinium Chloride). However, these antimicrobial agents are difficult to use for a long time because they can be caused by the appearance of resistant bacteria and myocerosis. In particular, chlorhexidine is used in the oral cavity at high concentrations or at low concentrations for a long time, causing side effects such as engraftment on the tongue or teeth, peeling of the oral mucosa, or taste disorders. In addition, a lot of fragrances, such as menthol and peppermint, are used.They use a masking effect that temporarily covers bad breath with the strong fragrance of the fragrance, rather than removing the bad breath. Insignificant These products are all products that can not be ingested in the form of rinsing out the mouth, it is urgent to develop oral disease prevention or treatment composition derived from natural substances that can be taken orally and does not cause side effects even after long-term use.

Constipation is caused by constipation due to lack of fiber intake and lack of exercise, as well as an increase in dietary standards and increased consumption of high-calorie foods such as high-fat and instant foods. The definition of constipation, when the stool is hard or rare to see the stool harder than normal, usually occurs when the stool less than 30g stool stool or less than 2 times a week can be diagnosed as constipation. Such constipation occurs when lack of water intake or food intake, especially low intake of fiber, lack of exercise, emotional instability and frequent endurance of stool. In addition, if the job or stress accumulated throughout the day sitting and working, the bowel movement is lowered and the ability to move the stool, constipation is frequent. As long as constipation lasts for a long time, the share of harmful bacteria living in the colon increases, and protein is used as a food source to produce ammonia gas, and the time that stool stays longer increases absorption of ammonia gas, resulting in odor. .

Among the previously reported deodorizing materials, there are few substances which exhibit deodorizing effect against monosulfide compounds such as allylmethyl monosulfide, and also the case of green tea extract (Japanese Patent No. 1330998) which shows a high effect on the deodorization of thiol compounds. It is known that the deodorizing effect on methyl monosulfide is insufficient. As such, monosulfide compounds such as allylmethyl monosulfide have a deodorizing mechanism that is significantly different from thiol compounds such as allyl mercaptan and methyl mercaptan, and thus are ineffective for monosulfide compounds. There are many.

It is known that benzaldehyde and cinnamon aldehyde react with monosulfide as a component derived from natural products, but these aldehydes have a strong characteristic peculiar aroma, and their practical use is limited, which makes them practical for monosulfide compounds. The development of high and safe deodorants is required.

As a deodorant component derived from natural products, the rosewood raspberry plant has been found to have a deodorizing effect on methyl mercaptan, a thiol compound, and trimethylamine, a nitrogen compound (Japanese Patent Publication No. 1823565). Deodorizing compositions are also disclosed as deodorizing materials for methyl mercaptan (Japanese Patent Laid-Open No. 05-269187). However, in both cases, the deodorizing effect on allylmethyl monosulfide, which is a monosulfide compound, has not been found.

Extracts from eucalyptus are likewise described as deodorants for hydrogen sulfide, ammonia, amines, mercaptans and nicotine (Japanese Patent Publication No. 60-261458), but the deodorizing effect on allylmethyl monosulfide, a monosulfide compound, has been found. none.

Although the effects of decaying meat fragments and deodorization in animal breeding rooms are known as air purifiers for noble trees (Japanese Patent Laid-Open No. 51-27882), these odors are generally hydrogen sulfide and mercaps generated by decomposition of animals and plants. Sulfur compounds such as carbons, nitrogen compounds such as ammonia and amines, and lower fatty acids are the main causes, and the deodorizing effect on allylmethyl monosulfide is not known.

The extract obtained from Hamamelis is disclosed as a deodorant for the axillary smell, foot odor, body odor and human odor of the human body (Japanese Patent Laid-Open No. 2000-186025), but the odor component is a lower fatty acid including isogyl acetate and Aldehydes are dominant and there are no reports of allylmethyl monosulfide. Therefore, the deodorizing effect on allylmethyl monosulfide by the Hamamelis derived component is not known.

In addition, other odors, such as urine and menstrual odors, may also occur.In particular, urine and menstrual odors are not covered by disposable absorbent products, and the disposable absorbent products are replaced in a timely manner. If you do not, the odor becomes more severe and may be used in close contact with the skin, which may adversely affect the skin.

Therefore, it is sufficient to develop a product that exhibits antibacterial and anti-inflammatory activity against Streptococcus aureus and Escherichia coli as an excellent deodorant effect on the human-derived odor including domestic odor and domestic dermatitis. There is no way.

One object of the present invention is to provide a disposable absorbent product comprising a deodorant composition excellent in antimicrobial, anti-inflammatory activity by producing a high content of polyphenols by fermentation, extraction of Ecklonia cava.

Another object of the present invention comprises a fermented Ecklonia cava extract as an active ingredient, and further comprises a plum extract and pork potato extract as an active ingredient to provide a disposable absorbent product comprising a deodorant composition excellent in antimicrobial and anti-inflammatory activity. It aims to do it.

Disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity according to the present invention, the absorbent pad in close contact with the human body to absorb the secretion, the absorbent pad containing the fermentation Ecklonia cava extract as an active ingredient Characterized in that it comprises a.

The disposable absorbent product is one or more selected from the group consisting of a super absorbent polymer, a liquid permeable top sheet, a liquid impermeable bottom sheet, a leakproof band and a coagulation band to fix and fix the absorbent pad to the human body. It may further include.

The deodorant composition may include a solvent as a fermentation Ecklonia cava extract 10 to 50% by weight and the balance.

The fermented Ecklonia cava extract may be obtained by alcohol extraction of Ecklonia cava fermentation obtained by fermenting Ecklonia cava.

The Ecklonia cava fermentation may be fermented with yeast.

The deodorant composition may further include a plum extract.

The deodorant composition may further include a plum extract in an amount of 1 to 20% by weight based on the total weight of the deodorant composition.

The deodorant composition may further include a pig potato extract.

The deodorant composition may further include a pig potato extract in an amount of 1 to 10% by weight based on the total weight of the deodorant composition.

According to the present invention, the polyphenol content of the fermented Ecklonia cava extract contains a higher content than before fermentation, and thus shows an effective effect, and includes such a fermented Ecklonia cava extract, and further includes a deodorant composition further comprising plum and swine potato extracts. Disposable absorbent product containing such deodorant composition is excellent antibacterial effect, in particular, antibacterial effect on the skin fungus, so as to solve the root cause due to the prevention or improvement of skin diseases, odor derived from the human body Helps to get rid of, especially bad smells.

1 is a graph showing the results of analyzing the inhibitory effect of nitrogen monoxide (Nitrite Oxide) production of the deodorizing composition constituting the present invention.

Figure 2 is a graph showing the results of measuring the cytotoxicity to the mouse-derived macrophages of the deodorant composition of the present invention.

Figure 3 is a graph showing the results of measuring the weight change of the female mouse according to a single toxicity test for mice of the deodorant composition of the present invention.

Figure 4 is a graph showing the results of measuring the changes in the insects of male mice according to a single toxicity test for the deodorant composition of the present invention.

Figure 5 is a graph showing the results of measuring the long-term change in weight of the female mouse according to a single toxicity test for the deodorant composition of the present invention.

Figure 6 is a graph showing the results of measuring the long-term change in weight of male mice according to a single toxicity test for the deodorant composition of the present invention.

7 is a graph illustrating the results of experiments on beneficial bacteria as a graph of the utilization of prebiotic (ie, food source) of pig potato in the deodorizing composition of the present invention.

FIG. 8 is a graph showing the results of experiments on harmful bacteria as a graph of the utilization of prebiotics (ie, food sources) of swine potatoes in the deodorizing composition of the present invention.

9 and 10 are graphs showing that the production amounts of IL-6 and TNF-α of the deodorizing composition of the present invention were suppressed in a concentration-dependent manner.

Disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity according to the present invention, the absorbent pad in close contact with the human body to absorb the secretion, the absorbent pad containing the fermentation Ecklonia cava extract as an active ingredient Characterized in that it comprises a.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity according to the present invention, the absorbent pad in close contact with the human body to absorb the secretion, the absorbent pad containing the fermentation Ecklonia cava extract as an active ingredient Characterized in that it comprises a.

The absorbent pad is in close contact with the human body, absorbs the secretion secreted from the human body, and stores it in the absorbent pad so as not to be discharged to the outside of the disposable absorbent product, and thus has no ability to control bowel / urination, such as infants or the elderly. It is applied to a weak person and makes it possible to lead a hygienic life by temporarily holding it in a disposable absorbent product despite sudden bowel movements / urination. The absorbent pad may be a spunlace diaper / physiological nonwoven fabric.

The disposable absorbent product is one or more selected from the group consisting of a super absorbent polymer, a liquid permeable top sheet, a liquid impermeable bottom sheet, a leakproof band and a coagulation band to fix and fix the absorbent pad to the human body. It may further include.

The super absorbent polymer is widely used in disposable absorbent products commonly supplied by domestic and international disposable absorbent product manufacturers, and super absorbent polymer (SAP) is about 500 to 1,000 times its own weight. It is a synthetic polymer material that has the ability to absorb water, and each developer has a different name such as SAM (Super Absorbency Material) and AGM (Absorbent Gel Material). Such super absorbent polymers have been put into practical use as physiological tools, and are currently used in gardening soil repair agents, civil engineering, building index materials, seedling sheets, food fresheners in addition to hygiene products such as paper diapers for children, and It is widely used as a material for steaming. As a method for producing such a super absorbent polymer, a method by reverse phase suspension polymerization or a method by aqueous solution polymerization is known. Reverse phase suspension polymerization is disclosed in, for example, Japanese Patent Laid-Open Nos. 56-161408, 57-158209, and 57-198714. As the method of aqueous solution polymerization, a thermal polymerization method of breaking and cooling the hydrogel polymer in a kneader having several shafts, and photopolymerization which simultaneously performs polymerization and drying by irradiating ultraviolet rays or the like on a belt with a high concentration of aqueous solution Methods and the like are known, but are not intended to be limited to these. In addition, the superabsorbent polymer may be, for example, those described in Korean Patent Laid-Open Publication No. 10-2015-0132035.

The liquid-permeable top sheet is located on the upper side of the absorbent pad, that is, the side that is in close contact with the skin, and functions to permeate the liquid secretion secreted from the human body to be absorbed by the absorbent pad positioned below the liquid absorbent. By passing through all or most of them, they do not remain in the top sheet, so that liquid secretions do not remain in the top sheet, which is in direct contact with the skin even when secreted from the human body.

The liquid-impermeable lower sheet is located on the lower side of the absorbent pad, that is, on the opposite side to the upper sheet. Once the liquid secretion is absorbed by the absorbent pad, the liquid secretion is absorbed to the outside of the absorbent product through the lower sheet even when external force is applied. It prevents the discharge or release and helps to lead a sanitary life.

The leakage preventing band serves as a jaw or an obstacle preventing the discharge of the discharge toward both legs around the absorbent pad, thereby preventing the discharge of the discharge to the outside of the absorbent product.

The coagulation band functions to fix the disposable absorbent article itself according to the present invention in close contact with the human body, including the absorbent pad, and is made of a magnetic strip and is integrally fixed to the absorbent article.

Such a super absorbent polymer, a liquid permeable top sheet, a liquid impermeable bottom sheet, a leakage prevention band, and one or more selected from the group consisting of a coagulation band, and further comprising an absorbent pad positioned between the top sheet and the bottom sheet. The disposable absorbent product may be understood to be the same and / or similar to that provided commercially by leading domestic and international manufacturers such as infant diapers or adult defenders.

Ecklonia cava used in the preparation of the fermented Ecklonia cava extract is a perennial algae belonging to Laminariales , Seaweed ( Alariaceae ). It inhabits about 10m of water along the coast of southern coast including Jeju Island of Korea. The length is 1 to 2m, the stem is cylindrical, and the base is root shaped. As edible algae, various functionalities such as antioxidant, anti-cancer and antihypertensive properties have been revealed, so it is well worth a new functional material. In particular, Ecklonia cava contains more than 14 polyphenolic substances, and studies on physiological activity are known to be excellent in anti-microbial activity as well as cytoprotective effect, antithrombotic activity, antioxidant activity, cardiovascular protection effect.

Fermented Ecklonia cava extract used in the present invention may be extracted after fermenting Ecklonia cava, which is a natural plant, preferably extracted with a lower alcohol having 1 to 4 carbon atoms, more preferably ethanol. Fermentation of the Ecklonia cava may preferably be yeast fermentation.

Preferably the fermentation Ecklonia cava extract is 1) YPD (Yeast Peptone Dextrose) medium sterilized for 1 to 30 minutes, preferably 10 to 20 minutes at a temperature within the range of 100 to 140 ℃, preferably 110 to 130 ℃ Inoculating Saccharomyces cerevisiae and preincubating for 1 to 3 days at 100 to 200 rpm, preferably 130 to 170 rpm, 30 to 40 ° C., preferably 35 to 38 ° C .; 2) washing the Ecklonia cava and drying it in the shade, and then pulverizing to prepare a powder form; 3) After mixing the Ecklonia cava powder and sterile water of step 2) in a ratio of 1: 2 to 7 by weight to obtain a fermentation mixture, the sugar content of 3 to 10% based on the total weight of the fermentation mixture Addition of components); 4) inoculating 4 to 6% (v / v) of the pre-cultivated yeast in the fermentation mixture of step 3) to ferment for 3 to 7 days at 35 to 38 ℃, 100 to 200 rpm to obtain a fermentation product ; 5) The supernatant is separated from the fermentation product of step 4) and lyophilized to obtain a fermentation powder, and then the fermentation powder is suspended in 10 to 50% ethanol and then 2 to 30 to 70 ° C. and 200 to 400 rpm. Extracting for 6 hours; 6) filtering the extract of step 5) and concentrating under reduced pressure, and then lyophilizing to obtain a fermented Ecklonia cava extract in powder form.

The deodorant composition may include a solvent as a fermentation Ecklonia cava extract 10 to 50% by weight and the balance. When the fermented Ecklonia cava extract is contained in less than 10% by weight, the effect of the deodorization rate and antibacterial ability of methylmercaptan may be insignificant, on the contrary, when the content exceeds 50% by weight, it does not dissolve more than the saturated concentration dissolved in the solvent and has a strong astringent taste. There may be issues that cause it.

The fermented Ecklonia cava extract may be obtained by alcohol extraction of Ecklonia cava fermentation obtained by fermenting Ecklonia cava.

The Ecklonia cava fermentation may be fermented with yeast.

The deodorant composition may further include a plum extract.

Plum used to prepare the extract of plum ( Prunus mune Sieb . et Zucc . ) Is a fruit of a plum tree, and the plum tree is a broad-leaved arborescent belonging to the Rosaceae family, and its height is about 4 to 5 m. White flowers or pale pink flowers, fruits ripening in May to June. The origin is known as the mountainous region of Sichuan and Hubei provinces in China. In Korea, it is planted in gardens or grown in orchards for ornamental purposes in the sub-central region of China. It has been documented that it controls the liver and the phlegm in the herbaceous wood, lowers the heat of the intestines, removes toxins in the body, makes it easier to see feces, and eliminates the smell of the mouth. Plum is known to have various effects such as fatigue recovery, constitution improvement, gastric juice secretion, appetite promotion, metabolic activation, and constipation prevention. In addition, due to the rich citric acid (citric acid) is known to have good antimicrobial activity, acidic stimulation of the salivary glands are known to stimulate the secretion of saliva.

Method for producing a plum extract used in the present invention comprises the steps of 1) separating and drying the seeds of the freshly cleaned blue plum and finely crushed flesh; 2) mixing the dried plum and water in step 1) in a weight ratio of 1: 2 to 7 and extracting at 50 to 100 ℃, 200 to 400 rpm for 2 to 6 hours; 3) filtering the extract of step 2) and concentrating to 60 to 70 brix with a reduced pressure concentrator; may be obtained through the method of producing a plum extract comprising a.

The deodorant composition may further include a plum extract in an amount of 1 to 20% by weight based on the total weight of the deodorant composition. When the extract of plum is included in less than 1% by weight, the effect of the deodorization rate and antibacterial ability of ammonia may be insignificant, on the contrary, if it exceeds 20% by weight, the acidity is strong due to high acidity, causing a strong acidity There may be.

The deodorant composition may further include a pig potato extract.

Pig potato ( Helianthus used in the preparation of the pork potato extract tuberosus L.) is a perennial herb of chrysanthemums and sunflowers, native to North America. Pork potatoes are made up of 80% water, 20% carbohydrates, 1-2% protein, minerals and vitamins. Unlike ordinary plants storing carbohydrates in the form of starch, it is known to store a carbohydrate substance called inulin. Inulin is a water-soluble fiber that is not degraded by gastric juice and digestive enzymes in animals. More than 80% of it reaches the large intestine, which is used as a growth substrate for intestinal microorganisms to play the role of prebiotics. Prebiotics microbiologically produce nutrients such as antibiotics, vitamins and growth promoters in the large intestine, improving intestinal function and enhancing mineral bioavailability. It is also known to help with bowel movements such as diarrhea and constipation prevention.

Method for producing a pig potato extract used in the present invention comprises the steps of 1) thinly cut and dried and dried finely washed pork potato; 2) mixing the dried plum and water in step 1) in a weight ratio of 1: 2 to 7 and extracting at 50 to 100 ℃, 200 to 400 rpm for 2 to 6 hours; 3) after filtering the extract of step 2) and concentrated in a vacuum condenser, and lyophilized, it may be obtained through a method for producing a pig potato extract comprising a.

The deodorant composition may further include a pig potato extract in an amount of 1 to 10% by weight based on the total weight of the deodorant composition. When the pork potato extract is included in less than 1% by weight, there may be a problem that does not help to play the role of prebiotics, if the amount exceeds 10% by weight, but helps to improve constipation, but excessive amount Rather, there may be problems that cause diarrhea.

The deodorant composition according to the present invention, preferably, 10 to 50% by weight of fermented Ecklonia cava extract, 1 to 20% by weight of plum extract, 1 to 10% by weight of pork potatoes and the residual amount may include a solvent.

The solvent may be an aqueous solution of glycerol at a concentration of 30 to 70%.

Glycerol is one of aliphatic trihydric alcohols having a molecular formula of C ₃ H ₅ (OH) ₃ , which is colorless, odorless, sweet, and sticky. It may be used as a drying agent, solvent, sweetener, or to preserve food. It is also called glycerin, rislin, propane-1,2,3-triol, 1,2,3-propenraiol, trihydroxypropane, glycerol, and glycyl alcohol. Colorless, transparent, sweet, sticky liquid with strong hygroscopicity. Strong cooling of very pure glycerol yields crystals with a melting point of 17.8 ° C, which is likely to cause supercooling. It is mixed in water and alcohol in any ratio, but insoluble in hydrocarbons. In 1779, Sweden's KW Scheele was discovered in the hydrolysis products of olive oil, and in 1836 the composition ratio was determined by TJ Pelouze, France. As esters with fatty acids, they are widely distributed in animal and plant systems in the form of fats and oils and lipids. It is made from by-products of soap production, but in recent years, it is synthesized from propylene produced by cracking, which is a cracking process of petroleum, through allyl chloride, or obtained as a product of glycerol fermentation (a variant of alcohol fermentation) of yeast. Etc. are known. It is converted into glucose in the liver in the human body and used as energy for cell metabolism, and has a calorie value of 4.32 kcal per gram. It is used to prevent drying of food by using hygroscopicity.

Since the deodorant composition according to the present invention is made of a natural material and has high safety, for example, it is possible to use it daily in combination with foods such as toothpaste, deodorant spray, or chewing gum, candy, tablets, and beverages.

The addition amount is most suited to add 0.5 to 1% by weight to the food or composition in consideration of the palatability of the food, may be added by 1% by weight or more in order to have an excellent deodorizing effect.

Therefore, all of the plants used as raw materials of the present invention have been used for a long time as natural substances, and there is no problem with regard to the safety of the deodorant composition using the extract of these plants as an active ingredient and the food and drink and composition containing the same.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Preparation Example 1: Preparation of Fermented Ecklonia cava Extract

Ecklonia cava was fermented and extracted for use in the deodorant composition according to the present invention. In order to ferment Ecklonia cava yeast ( S. cerevisiea ) was inoculated in YPD (Yeast Peptone Dextrose) medium and pre-incubated at 37 ° C. and 150 rpm for 2 days. The ratio of Ecklonia cava powder and sterile water was mixed at a weight ratio of 1: 5, and then 5% sugar was added and mixed. After inoculating 5% (v / v) of the yeast cultured in the mixture was fermented for 5 days at 150rpm, 37 ℃. After the fermentation was completed, the culture supernatant was taken by centrifugation at 8,000 rpm for 10 minutes and lyophilized. The lyophilized powder was suspended in 30% ethanol and extracted at 50 ° C. and 300 rpm for 4 hours. The extract was filtered through a paper filter, concentrated, and lyophilized to obtain fermented Ecklonia cava extract. The Ecklonia cava extract was dried, pulverized, and suspended in purified water, and then extracted at 50 ° C. and 300 rpm for 4 hours. The extract was concentrated by filtration through a paper filter and lyophilized to obtain Ecklonia cava extract.

Table 1 shows the results of measuring the total polyphenol content between the Ecklonia cava extract and the fermented Ecklonia cava extract.

	Total Polyphenol Content (%)
Ecklonia cava extract	30.18%
Fermented Ecklonia cava extract	42.53%

Preparation Example 2 Preparation of Plum Extract

In order to use plum in the deodorant composition according to the present invention, extraction solvent was used as water. Dry and pulverized plums were mixed with water at a weight ratio of 1: 5 and extracted at 80 ° C. and 300 rpm for 4 hours. The plum extract was filtered through a paper filter and concentrated to 65 brix with a vacuum concentrator to obtain a plum extract.

Preparation Example 3: Preparation of Pork Potato Extract

In order to use the pork potato in the deodorant composition according to the present invention, the extraction solvent was used as water. Dry ground pulverized pork potatoes were mixed with water at a weight ratio of 1: 5 and extracted at 80 ° C. and 300 rpm for 4 hours. Porcine potato extract was filtered through a paper filter, concentrated to 30 brix with a vacuum concentrator, and lyophilized to obtain a pork potato extract.

Example 1

300 g of fermented Ecklonia cava extract of Preparation Example 1 was mixed with 700 g of 50% aqueous solution of glycerol to obtain a deodorant composition constituting the present invention, which was applied to an absorbent pad and then dried to have antibacterial and anti-inflammatory activity according to the present invention. Deodorant compositions were prepared.

Example 2

For comparison, 300 g of the fermented Ecklonia cava extract of Preparation Example 1 and 100 g of the plum extract of Preparation Example 2 were mixed with 600 g of an aqueous 50% glycerol solution to obtain a deodorant composition constituting the present invention, and then applied to an absorbent pad, followed by drying. To prepare a deodorant composition having antibacterial and anti-inflammatory activity according to the present invention.

Example 3

300 g of fermented Ecklonia cava extract of Preparation Example 1 and 50 g of Pork Potato Extract of Preparation Example 3 were mixed with 650 g of an aqueous 50% glycerol solution to obtain a deodorant composition constituting the present invention. A deodorant composition having antibacterial and anti-inflammatory activity according to the invention was prepared.

Example 4

300 g of fermented Ecklonia cava extract obtained in Preparation Examples 1 to 3, 100 g of plum extract, 50 g of pork potato extract and 550 g of 50% aqueous solution of glycerol were mixed to obtain a deodorant composition constituting the present invention, and then applied to an absorbent pad. Drying to prepare a deodorant composition having an antibacterial, anti-inflammatory activity according to the present invention.

Comparative Example 1

For comparison, 300 g of Ecklonia cava extract of Preparation Example 1 was mixed with 700 g of 50% aqueous solution of glycerol was used as Comparative Example 1.

Comparative Example 2

For comparison, 300 g of the Ecklonia cava extract of Preparation Example 1 and 100 g of the plum extract of Preparation Example 2 were used as Comparative Example 2, which was mixed with 600 g of 50% aqueous glycerol solution.

Comparative Example 3

For comparison, 300 g of Ecklonia cava extract of Preparation Example 1 and 50 g of Porcine Potato Extract of Preparation Example 3 were used as Comparative Example 3 in which a composition of 650 g of 50% aqueous glycerol solution was mixed.

Comparative Example 4

For comparison, 300 g of Ecklonia cava extract of Preparation Example 1, 100 g of Maesil Extract of Preparation Example 2, and 50 g of Pork Potato Extract of Preparation Example 3 were used as a Comparative Example 4 in which 550 g of a 50% aqueous solution of glycerol was mixed.

Experimental Example 1: Deodorizing effect-test method for gas detector tube

Deodorizing composition of the present invention was measured using a gas detector tube (Gastec GV-110S, 71H, Korea) to determine the deodorizing effect on methyl mercaptan and ammonia (ammonia).

The vial was mixed and stirred until the final concentration of the deodorant composition of the present invention and methyl mercaptan (Wako pure chemicals Co., Japan) was 100 ppm, and then left at 37 ° C. for 10 minutes. After standing, the concentration of methylmercaptan was measured using a gas detector tube. At this time, distilled water without the deodorant composition was used as a control. In addition, the method of ammonia (Daejeong, Korea) was also measured by the same method as the above-mentioned method.

Odor removal activity of Ecklonia cava extract was calculated according to the following formula.

Odor elimination activity (%) = [(C-S)] * 100

In the above formula, C is the measured concentration of the control group, S is the measured concentration of the experimental group to which the deodorant composition of the present invention is added.

As a result, it was confirmed that the deodorant composition of the present invention removes the smell of methylmercaptan by 50% and removes the smell of ammonia by 75%, and the results are shown in Table 2 below.

division	Methylmercaptan	Methylmercaptan odor removal activity (%)	ammonia	Ammonia Odor Removal Activity (%)
Control (ppm)	100	0	100	0
Comparative Example 1 (ppm)	90	10	100	0
Example 1 (ppm)	50	50	80	20
Comparative Example 2 (ppm)	90	10	50	50
Example 2 (ppm)	50	50	25	75
Comparative Example 3 (ppm)	90	10	100	0
Example 3 (ppm)	55	45	80	20
Comparative Example 4 (ppm)	90	10	50	50
Example 4 (ppm)	50	50	25	75

Experimental Example 2: Simplified Clinical Experiment

In order to investigate the anti-odor effect, 15 dogs (Maltis, 3 months, males, dog and cat dog shop, Korea) were allowed to ingest water autonomously for 1 week, and the deodorant composition of the present invention (Example 4) for the following 1 week. ) Was diluted to 1% by weight to allow for autonomous intake. Breeding conditions were carried out after setting the temperature to 25 ℃, the solid feed (Royal Canine Starter, France) was provided by dividing 5% of the average body weight twice a day. Ammonia status was measured by indo-phenol method in order to measure the ammonia concentration by collecting the stools before and after the deodorant composition, the results are shown in Table 3 below.

division	Before Intake (ppm)	After ingestion (ppm)
Object 1	403.23	224.12
Object 2	447.72	369.09
Object 3	388.61	253.76
Object 4	510.28	282.33
Object 5	355.66	278.79
Object 6	451.44	314.24
Object 7	461.27	343.53
Object 8	399.54	316.07
Object 9	420.31	184.77
Object 10	388.70	264.92
Object 11	285.04	240.88
Object 12	306.62	190.22
Entity 13	506.63	252.51
Entity 14	490.46	370.37
Object 15	412.66	398.87

As shown in Table 3, 15 dogs were found to have decreased ammonia concentration before and after ingestion. Although there are differences among individuals, the average tendency is to decrease by about 30%.

Experimental Example 3: Measurement of Lipopolysaccharide (LPS) -Induced Nitric Oxide (NO) Production

Nitric oxide concentration in the culture was measured using a Greries reaction. First, RAW 264.7 cells derived from 1 * 10 ⁶ cells / well of mouse macrophages in a 96 well culture plate using Fetal Bovine Serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM) containing antibiotics. After aliquoting, the cells were incubated for 24 hours in a 37 ° C., 5% carbon dioxide (CO ₂ ) incubator. After 24 hours, the medium used for the previous culture was removed, and after 1 hour of dispensing the deodorant composition of the present invention (Example 4) in fresh DMEM medium containing no FBS and antibiotics, respectively, 1 μg / ml LPS was treated and incubated for 24 hours. Nitrogen monoxide produced during the cultivation was measured by the concentration of total nitrogen monoxide present in the cell culture medium using a grease reagent, and 50 μl of the cell culture supernatant and 50 μl of the grease reagent were mixed and reacted in a 96 well culture plate for 10 minutes. The absorbance was then measured at 540 nm using an ELISA reader. The control group was phosphate buffered saline (PBS: Phosphate Buffered Saline). Nitrogen monoxide scavenging ability (%) was shown using the formula of (the absorbance of the reaction group which added the 1 sample / the absorbance of the control group which did not add the sample). IC ₅₀ (ppm), a concentration at which 50% of nitrogen monoxide is removed.

As a result, as shown in Figure 1, when compared to the deodorant composition of the present invention and the control group it was found that the amount of nitrogen monoxide produced. An IC ₅₀ value of 50% scavenging of nitrogen monoxide was found to be 174.46 ppm. It can be seen that the deodorizing composition of the present invention has an excellent effect on suppressing nitrogen monoxide.

Experimental Example 4: COX-2 Enzyme Inhibition

10 μl of COX-2 enzyme, 10 μl of heme (porphyrin iron complex), and 160 μl of reaction buffer were added thereto, and 10 μl of the deodorant composition of the present invention (Example 4) was added to each concentration, followed by 37 ° C. for 5 minutes. Stabilized at. 10 μl of arachidonic acid was added thereto and reacted at 37 ° C. for 2 minutes, and then 10 μl of 1M hydrochloric acid was added to stop the reaction. SnCl ₂ Add 20 μl, mix well, and leave at room temperature for 5 minutes. Dilute the reaction solution with EIA buffer 2000 times and 4000 times, and dispense 50 μl into a 96 well plate coated with prostaglandin antibody. 50 μl of screening AChE tracer) and 50 μl of antiserum were added and reacted at room temperature for 18 hours. The wells were washed five times with buffer, 200 μl of Ellman's reagent was added and developed for 60 minutes, and then absorbance was measured at 410 nm. At this time, ibuprofen (Ibuprofen), which is known to inhibit COX-2 enzyme, was used as a control. The degree of COX activity inhibition was expressed as IC ₅₀ (ppm), a concentration that inhibits 50% using the manufacturer's method.

Experimental results, the results of the experiment whether the deodorant composition of the present invention inhibits the COX-2 enzyme is shown in Table 4 below. IC ₅₀ values of 50% inhibition of COX-2 enzyme in the control and deodorant compositions were 16.3 ppm and 101.39 ppm, respectively. The IC ₅₀ value is higher than that of ibuprofen, which is a synthetic drug, but it can be seen that the COX-2 inhibitory effect of the deodorant composition composed only of natural substances is excellent.

IC 50 (ppm)	COX-2
Control group (ibuprofen)	16.3
Example 1	101.39

Experimental Example 5: DPPH radical scavenging activity

After dissolving the deodorant composition of the present invention (Example 4) by concentration, it was mixed with DPPH (0.2 mM ethanol solution) (Sigma, USA) in the same amount. After 30 minutes of reaction at room temperature, absorbance was measured at a wavelength of 520 nm. Vitamin C was used as a control to compare relative antioxidant effects. As the DPPH radical scavenging activity (%), a formula for (absorbance of 1-sample added group / absorbance of sample-free group) was used. The SC ₅₀ value indicates the minimum concentration required in ppm to remove 50% of the generated radicals, and the results are shown in Table 5 below.

DPPH radical scavenging activity	SC 50 (ppm)
Control group (vitamin C)	4.52
Deodorant composition	64.01

As a result, as shown in Table 5, it was confirmed that the SC ₅₀ value of the vitamin C and the deodorant composition of the control group was 4.52ppm, 64.01ppm, respectively, DPPH radical scavenging ability of the deodorant composition of the present invention is about 1 Antioxidant effect of / 14 times was confirmed.

Experimental Example 6: Measurement of Cytotoxicity

MTT analysis was performed to determine the toxicity of Raw 264.7 cells, a mouse-derived macrophage line of the deodorant composition (Example 4) of the present invention.

Toxicity of macrophages was analyzed by MTT [(3- (4,5-dimethyl thiazol-2yl) -2,5-diphenyl tetrazolium bromide) (Sigma, MO, USA)]. Raw264.7 cells were dispensed at 1 ⁴ cells / ml per well of 96 wells and then incubated at 37 ° C., 5% CO ₂ for 24 hours. After 24 hours, the medium used for the previous culture was removed and the samples were incubated at 37 ° C., 5% CO ₂ for 24 hours by treating each concentration (μg / ml), respectively. After 24 hours, the medium was removed and 0.2% MTT solution was added per well for 3 hours at 37 ° C. and 5% CO ₂ . After the reaction, all supernatants were removed, 200 μl each of DMSO was added, and all the formazan produced at room temperature was dissolved for 30 minutes. The change in absorbance was measured at 570 nm using an ELISA reader. Cell survival rate (%) was expressed using the following formula. Phosphate buffered saline was used as a control.

Cell viability (%) = (absorbance of sample treatment / absorbance of control)

As a result, as shown in Figure 2, the cytotoxicity to the deodorant composition of the present invention was confirmed that there is no toxicity even at a high concentration of 1000ppm.

Experimental Example 7: Single toxicity test

The experimental animals were rats of 7-week-old Sprague Dawley (SD), rats (Samtago, Korea). The experimental groups were 5 males and 5 males, respectively. These animals did not observe any abnormal symptoms at the time of acquisition and during the adaptation period. Breeding conditions were carried out after setting the temperature 22 ℃, relative humidity 45%, lighting time 12 hours (6 am to 6 pm), using a solid feed for experimental animals (Samgo, Korea) and water purification system Tap water was allowed to intake freely. The mice were fasted 12 hours before the administration of the sample, and orally administered at two concentrations of 2000 mg / kg and 1000 mg / kg, which are usually used as a single toxic oral dose of health functional food, and then observed for 14 days. Clinical symptoms were observed according to the Food and Drug Administration's Toxicity Test Criteria and OECD Test Guideline 420 (TG 420). That is, all the experimental animals were observed every hour for 6 hours after administration on the day of administration, and the change of general condition of the animal, the expression of intoxication symptoms and the presence of death were observed once a day from the following day to 14 days. In addition, the weight change of the test substance (deodorant composition according to the present invention (Example 4)) and the 1, 3, 7, 14 days was measured. After the end of the test, the animals were anesthetized and lethal, and then visually observed for abnormalities in appearance and internal organs.

As a result of the experiment, as shown in Figures 3 to 6, there were no deaths in males and females according to the administration, and when observed visually once a day for 14 days from the day and the following day, specific behavior in the experimental group compared to the control group There was no change. In addition, as shown in Figures 3 to 6, 14 days after the administration of the sample, the change in the weight of the organ after weight change and autopsy there was no significant change compared to the control. Therefore, it can be seen that the deodorant composition of the present invention has no animal single toxicity.

Experimental Example 8: Use of Pig Potatoes as Prebiotics (ie, Food Sources)

Growth curve was measured to determine the effect of the deodorant composition of the present invention (Example 4) on the growth of representative beneficial bacteria (lactic acid bacteria: Bifidobacteriu bifidum ) and harmful bacteria ( Clostridium perfrigens ) in the human intestine. Each used medium composition was added 10% deodorant composition to Reinforced clostridial medium, and 10% of deodorant composition was added to Lactobacilis MRS broth and cultured for 72 hours. . The degree of propagation of the bacteria was measured at an absorbance of 660 nm at 12 hour intervals using a spectrophotometer, and the results are shown in FIGS. 7 and 8.

As a result, the growth curve was measured by adding 10% of the deodorant composition (Example 4). As a result, it was found that the growth rate of B. bifidum , a beneficial bacterium, was increased, whereas the growth of harmful bacteria ( C. perfrigens ) was observed . It was found to be suppressed.

Experimental Example 9: Growth Inhibition Effect of Food Poisoning and Other Skin Disease-causing Microorganisms

Whether the deodorant composition of Example 4 is effective in inhibiting the strain using two kinds of food poisoning-inducing microorganisms and at the same time dermatological bacteria Streptococcus aureus and A. coli ( E. coli ) Investigate.

The investigation used a test method using a liquid medium. The Streptococcus aureus ( S. aureus ) was inoculated in MHB (Mueller Hinton Broth) medium, Aceriki coli ( E. coli ) in the LBB (Luria-Bertani Broth) medium, respectively, 24 ℃ at 37 ℃, 150 rpm After incubation for a time, the viable cell count was measured by the smear plate method. That is, the strains were inoculated in a liquid medium so as to be 1 × 10 ⁷ CFU / mL, and the deodorant composition of Example 4 was added to each concentration, followed by incubation at 37 ° C. and 150 rpm for 24 hours (MIC). ) Was measured.

As a result, Streptococcus aureus each MIA values are shown as 5000 ppm and 20000 ppm for (S. aureus) and aeseo Ricky air coli (E. coli) it was confirmed that the excellent antibacterial activity.

Examples 5-7

After dissolving the deodorant composition of Example 2 by concentration (Example 5: 1%, Example 6: 5%, Example 7: 10%), 2 ml of the obtained solution was spunlace diaper / physiological nonwoven fabric ( 10 * 10 cm, 50 g / m 2) and dried at 60 ° C. for 1 hour.

Comparative Example 5

The same procedure as in Example 5 was conducted except that distilled water was used instead of the deodorant composition as a control.

Experimental Example 10: Diaper Odor Removal Effect-Ammonia

The nonwoven fabrics of Examples 5 to 7 and the nonwoven fabrics of Comparative Example 5 were each placed in an airtight container (3 L), and then the 1% ammonia solution was added so that the initial concentration was 100 ppm. Was measured using a gas detector tube (Gastec GV-100S, Japan).

Deodorizing activity was calculated according to the following equation.

Deodorization activity (%) = [(C-S)] / C × 100

In the above formula, C is the measured concentration of Comparative Example 5, S is the measured concentration of Examples 5 to 7.

As a result, as shown in Table 6, in the case of Comparative Example 5, there was no change in the concentration of the initially introduced ammonia, when applying the deodorant composition of the present invention, a decrease in the ammonia concentration was measured, in particular 10% applied When it was found that after 1 hour to remove the smell of ammonia 100%. The results are shown in Table 6 below.

ammonia	Comparative Example 5 (ppm)	Example (ppm)	Deodorization Activity (%)
Example 5	100	60	40
Example 6	100	12.5	87.5
Example 7	100	0	100

Experimental Example 11: Physiological Odor Removal Effect-Trimethylamine

Trimethylamine (TMA: trimethylamine) is a substance produced by the body's metabolic processes. Under the control of sex hormones, women have a smell of trimethylamine before and after menstruation.

Examples 5 to 7 and Comparative Example 5 were each placed in an airtight container (3 L), followed by addition of 1% trimethylamine solution to an initial concentration of 70 ppm, and gas detection of the residual concentration of trimethylamine gas produced after 1 hour. It measured using the tube (Gastec GV-100S, Japan).

Deodorizing activity of the deodorant composition was calculated in the same manner as in Experimental Example 10.

As a result, as shown in Table 7, in the case of Comparative Example 5, there was no change in the concentration of trimethylamine initially charged, but when the deodorant composition of the present invention is applied, a decrease in the concentration of trimethylamine was measured, in particular 10% When applied, it can be seen that after 1 hour to remove 95% of the smell of trimethylamine. The results are shown in Table 7 below.

Trimethylamine	Comparative Example 5 (ppm)	Example (ppm)	Deodorization Activity (%)
Example 5	70	56	20
Example 6	70	10.5	85
Example 7	70	3.5	95

Experimental Example 12: Removal effect of diaper and sick house syndrome-induced acetaldehyde

Volatile organic compounds such as acetaldehyde and formaldehyde, which are produced in furniture adhesives, cause sick house syndrome, which causes eye, nose and skin irritation. The deodorizing effect of the deodorant composition of Example 2 on acetaldehyde was measured as follows. After dissolving the deodorizing composition of Example 2 by concentration (10%, 20%, 40%), 2 ml was added to a spunlace nonwoven fabric (10 * 10 cm, 50 g / m 2) and dried at 60 ° C. for 1 hour. Instead of the deodorant composition, put the nonwoven fabric (control group) to which distilled water was applied and the nonwoven fabric to which the deodorant composition was applied for each concentration (10, 20%, 40%) were put in a closed container (3 ℓ), respectively, and the initial concentration was 100% by adding 1% acetaldehyde solution. The residual concentration of acetaldehyde gas produced in ppm and after 2 h was measured using a gas detector tube (Gastec GV-100S, Japan).

Deodorizing activity of the deodorant composition was calculated in the same manner as in Experimental Example 10.

As a result, it was found that 70% of the odor of acetaldehyde was removed after 2 hours when 40% of the deodorant composition of the present invention was applied. The results are shown in Table 8.

Acetaldehyde	Control (ppm)	Deodorant composition (ppm)	Deodorization Activity (%)
10% Deodorant Composition	100	80	20
20% Deodorant Composition	100	60	40
40% Deodorant Composition	100	30	70

Experimental Example 13: New house syndrome-induced odor removal effect-formaldehyde

The removal effect of the deodorant composition of Example 2 on formaldehyde, which is a substance causing the sick house syndrome, was measured by the following method. That is, the deodorant composition was dissolved at different concentrations (10%, 20%, 40%), and 2 ml was added to a spunlace nonwoven fabric (10 * 10 cm, 50 g / m 2) and dried at 60 ° C. for 1 hour. Instead of the deodorant composition, the nonwoven fabric (control group) with distilled water and the nonwoven fabric with deodorant composition (10%, 20%, 40%) were put in a closed container (3 L), respectively, and the initial concentration was added by adding 10% formaldehyde solution. The residual concentration of formaldehyde gas, which was brought to 80 ppm and after 2 hours, was measured using a gas detector tube (Gastec GV-100S, Japan).

Deodorizing activity of the deodorant composition was calculated in the same manner as in Experimental Example 10.

As a result, when 40% of the deodorant composition of the present invention was applied, it was found that 50% of the smell of formaldehyde was removed after 2 hours. The results are shown in Table 9.

Formaldehyde	Control (ppm)	Deodorant composition (ppm)	Deodorization Activity (%)
10% Deodorant Composition	80	70	12.5
20% Deodorant Composition	80	60	25
40% Deodorant Composition	80	40	50

Experimental Example 14 Stability to Deodorization Efficacy of Deodorant Composition-Ammonia

Stability of the deodorizing composition of Example 2 for ammonia removal efficacy was measured by the following method. The deodorant composition was dissolved at 10%, and then 2 ml of the spunlace nonwoven fabric (10 * 10 cm, 50 g / m 2) was dried and stored at 60 ° C. for 1 hour, 48 hours, and 120 hours, respectively. 1% ammonia solution was added to the sealed container (3 L) to find a concentration of 100 ppm, and based on this, the experiment was carried out as follows. Instead of the deodorant composition, put the nonwoven fabric (control group) with distilled water and the nonwoven fabric with 10% deodorant composition (dry for 1 hour, 48 hours, 120 hours) in a closed container (3 ℓ), and then add 1% ammonia solution. The residual concentration of ammonia gas, which was brought to 100 ppm and after 1 hour, was measured using a gas detector tube (Gastec GV-100S, Japan).

As a result, when the deodorant composition of 10% of the present invention was applied and stored at 60 ° C. for 120 hours, 97.5% of the smell of ammonia was removed, and the deodorizing effect was similar to that of the 1 hour dry sample (100%). Therefore, when the deodorant composition of the present invention was stored for up to 120 hours at 60 ℃ it can be seen that the deodorizing effect is stable and the results are shown in Table 10.

ammonia	Control (ppm)	Deodorant composition (ppm)	Deodorization Activity (%)
1 hour drying	100	0	100
48 hours drying	100	0	100
120 hours drying	100	2.5	97.5

Experimental Example 15 Stability to Deodorization Efficacy of Deodorant Composition-Trimethylamine

Stability of trimethylamine removal efficacy of the deodorant composition of the present invention was measured by the following method. The deodorant composition was dissolved at 10%, and then 2 ml of the spunlace nonwoven fabric (10 * 10 cm, 50 g / m 2) was dried and stored at 60 ° C. for 1 hour, 48 hours, and 120 hours. Instead of the deodorant composition, put the nonwoven fabric (control group) with distilled water and the nonwoven fabric with 10% deodorant composition (dry for 1 hour, 48 hours, 120 hours) in a closed container (3 L), respectively, and then add 1% trimethylamine solution. Was 70 ppm and the residual concentration of the trimethylamine gas produced after 1 hour was measured using a gas detector tube (Gastec GV-100S, Japan).

As a result, when the deodorant composition 10% of the present invention was applied and stored at 60 ° C. for 120 hours, 90% of the odors of trimethylamine were removed, indicating a similar effect to that of the 1 hour dry sample (95%). Therefore, when the 10% deodorant composition was stored at 60 ° C. for up to 120 hours, the deodorizing effect was found to be stable. The results are shown in Table 11.

Trimethylamine	Control (ppm)	Deodorant composition (ppm)	Deodorization Activity (%)
1 hour drying	70	3.5	95
48 hours drying	70	3.5	95
120 hours drying	70	7	90

Experimental Example 16: Measurement of LPS-induced IL-6 and TNF-α Production

After inducing an inflammatory response with LPS, the concentrations of IL-6 and TNF-α in the cell culture were measured as follows. First, RAW 264.7 cells derived from 1 × 10 ⁶ cells / ml of mouse macrophages were cultured in a 24 well culture plate using DMEM medium, and then cultured in a 37 ° C., 5% CO ₂ incubator for 24 hours. After 24 hours, the deodorant composition of the present invention was dispensed in fresh DMEM medium by concentration (25, 50, 100, 200 µg / ml), and then incubated for 24 hours by treatment with 1 µg / ml of LPS. IL-6 and TNF-α in the culture were measured using an ELISA kit (R & D Systems, Minneapolis, MN). The control group was PBS (Phosphate Buffered Saline).

As a result, as shown in Figures 9 and 10, when compared to the deodorant composition of the present invention and the control group, the production amount of IL-6 and TNF-α was shown to be inhibited in a concentration-dependent manner. It was confirmed that the deodorant composition of the present invention has an excellent effect on inhibiting inflammation by inhibiting IL-6 and TNF-α.

The present invention can be used particularly in the industry of manufacturing and using health foods and hygiene products that require deodorant function.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

Claims (9)

1. It includes an absorbent pad that is in close contact with the human body to absorb the secretion, and the absorbent pad includes a deodorant composition comprising the fermented Ecklonia cava extract as an active ingredient. product.
2. The method of claim 1,

   At least one disposable absorbent product is selected from the group consisting of a super absorbent polymer, a liquid permeable top sheet, a liquid impermeable bottom sheet, a leak-proof strip and a coagulation band to fix and fix the absorbent pad to the human body. Disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that it further comprises.
3. The method of claim 1,

   The deodorant composition is a disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that the fermentation Ecklonia cava extract contains 10 to 50% by weight and the solvent as a residual amount.
4. The method of claim 1,

   The fermentation Ecklonia cava extract is a disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that the fermentation of Ecklonia cava fermentation obtained by alcohol extraction.
5. The method of claim 4, wherein

   Disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that the Ecklonia cava fermentation is fermented into yeast.
6. The method of claim 1,

   The deodorant composition is a disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that it further comprises a plum extract.
7. The method of claim 6,

   The deodorant composition is a disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that it further comprises an amount of 1 to 20% by weight of plum extract based on the total weight of the deodorant composition.
8. The method of claim 1,

   The deodorant composition is a disposable absorbent product comprising a deodorant composition having antibacterial and anti-inflammatory activity, characterized in that it further comprises a pig potato extract.
9. The method of claim 8,

   The deodorant composition is a disposable absorbent product comprising a deodorant composition having antimicrobial and anti-inflammatory activity, characterized in that it further comprises 1 to 10% by weight of the pig potato extract based on the total weight of the deodorant composition.

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