KR20100001122A - A antibacterial compositions comprising an extract of ecklonia cava - Google Patents

Abstract

PURPOSE: An antibacterial composition containing Ecklonia cava extract is provided to ensure high antibacterial activity to main bacteria causing food poisoning and prevent or treat food poisoning. CONSTITUTION: An antibacterial composition contains Ecklonia cava extract as an active ingredient. The Ecklonia cava extract is isolated using water, alcohol of 1-4 carbon atoms, ethylacetate, hexane, or dichloromethane. A food preservative, composition for preventing or treating food poisoning, food composition for preventing or treating food poisoning and washing composition contains the antibacterial composition containing the Ecklonia cava extract as an active ingredient.

Description

Antibacterial composition containing Ecklonia cava extract {A ANTIBACTERIAL COMPOSITIONS COMPRISING AN EXTRACT OF ECKLONIA CAVA}

The present invention is Ecklonia cava ) relates to an antimicrobial composition comprising the extract as an active ingredient.

Most seafood products have been sold in the past, but recently processed seafood products that have been easily packaged for consumption by removing heads, intestines, and bones have become popular. The processed fish products as described above can be easily eaten from the consumer side, and can increase the value added from the producer side, the production amount is increasing. More specifically, according to the Ministry of Maritime Affairs and Fisheries data (as of the end of December 2003), there are a total of 2295 fish processing companies nationwide, and the annual output of processed fishery products is 1,137,717 tons. The government also plans to support the seafood processing industry by fostering the mountain processing industry and establishing a comprehensive marine processing complex.

On the other hand, as the standard of living in Korea is improved and the mass production of food is started due to the improvement of diet, consumers' interest in food safety is increasing. In particular, in the 21st century, called the “well-being age,” foods with emphasis on functionality and hygiene have received high responses from consumers, and even in the case of aquatic products, the demand for fishery products such as refrigeration and freezing, according to the high quality and convenience of products, as described above. Is increasing rapidly. Along with such a surge in demand for processed fish products, there is also a growing interest in the safety of fish products, which is due to the characteristics of fish products, which may occur if they are improperly managed during processing, distribution, storage or consumption. This is because the proliferation can greatly affect food decay and quality change.

In general, as soon as the food is left in nature, the appearance of flavor, color, etc. changes, and the ingredients are decomposed to reduce the nutritional value and lose the value of food. This is because the deterioration of the food is caused by the action of microorganisms such as enzymes and bacteria, yeast and mold that survive in the food.

In order to solve this problem, various treatment methods for inhibiting the growth of microorganisms have been proposed.

Conventionally, sterilization was performed under high temperature and high pressure, but in this method, there is a problem in that nutrients contained in food are destroyed or texture is degraded due to treatment conditions such as high temperature and high pressure. In addition, in the case of irradiating ultraviolet rays and / or radiation in a similar manner, the bactericidal effect on the harmful microorganisms contained in the food is excellent, but at the same time, there is a problem that the destruction of various components of the food is accompanied.

As another method, there is a method of inhibiting the growth of microorganisms by treating a preservative or preservative. Currently used preservatives or preservatives include synthetic preservatives such as dihydroacetic acid, propionic acid, sorbic acid or benzoic acid, organic acids or alcohols such as acetic acid, lactic acid, malic acid or glycine, and the like, which are mainly processed alone or in combination to foods. have.

Among the preservatives, synthetic preservatives have an advantage of excellent preservation effect, but synthetic materials such as synthetic preservatives or synthetic preservatives may contain toxic substances harmful to the human body, and when accumulated in the body by ingestion or continuous ingestion, Since it may have a harmful effect on the human body such as a problem of chronic toxicity or carcinogenesis or mutagenesis, there is a disadvantage that the range of use, such as the type and amount of food that can be used, is limited. Specifically, in the food field, the synthetic preservatives are used as food preservatives only in a limited range, since they can be used for sterilization and preservation only in the food additive or food additives allowed in the food field. It is true.

In addition, organic acids, such as lactic acid or acetic acid, have a certain degree of antibacterial activity at the concentration generally used, but has a disadvantage in that there is little sterilizing power, and when used in excess to enhance the preservation effect, the taste and flavor of food The disadvantage is that it gives. In particular, in the case of acetic acid having the highest antimicrobial activity among organic acids, there is a disadvantage in that it affects the smell of food when added to the food due to the unique pickling, its use range is limited.

In the case of seafood products, conventionally, the method of increasing the preservation and shelf life through salting process has been used, but salting treatment is a process that focuses on flavoring rather than hygienic processing of fishery products, and has a disadvantage in that the effect of inhibiting the growth of microorganisms is insufficient. have. In particular, the harmful microorganisms such as staphylococcus aureus and enteritis vibrio bacteria, which cause food poisoning when aquatic products are ingested or aquatic products are marine marine strains that can grow even at high salinity, and thus the food preservation effect of the salt processing on the processed marine products is insufficient.

Therefore, there is a growing demand for development of an antimicrobial agent or food preservative for foods derived from improved natural substances that are excellent in antimicrobial power and not harmful to the human body without destroying nutrients or deteriorating texture. In particular, the three sides of the sea, considering the geographical environment of Korea, which is rich in marine resources, the composition or composition having excellent antimicrobial activity against microorganisms in order to induce decay or food poisoning of food from marine natural substances including marine flora and fauna There is a growing demand to develop methods for preserving used seafood and fish products.

Therefore, the technical problem to be achieved by the present invention is to solve the problems of the prior art, Ecklonia cava ) to provide an antimicrobial composition for microorganisms, including the extract as an active ingredient, a food preservative composition comprising the antimicrobial composition as an active ingredient, and a functional food composition for preventing or improving food poisoning comprising the antimicrobial composition as an active ingredient. will be.

In order to achieve the above object, the present invention is Ecklonia cava ) provides an antimicrobial composition against microorganisms to include the extract as an active ingredient.

In addition, the present invention provides a food preservative composition comprising the antimicrobial composition as an active ingredient.

The present invention also provides a composition for treating or preventing food poisoning comprising the antimicrobial composition as an active ingredient.

In addition, the present invention provides a functional food composition for preventing or improving food poisoning comprising the antimicrobial composition as an active ingredient.

The inventors of the present invention, by using seaweed, especially seaweed, which is rich in the south coast of Korea, including Jeju Island, have antimicrobial activity against harmful microorganisms, have antioxidant activity, and have excellent food preservation effect, and prevent, improve or treat diseases such as food poisoning. While researching to find a substance that has an effect, E. coli hot water extract is responsible for diseases such as food rot or food poisoning, Staphylococcus aureus , Vibrio parahemolyticus , V. parahemolyticus , Listeria It has excellent antibacterial activity against Listeria monocytogenes , Bacillus subtilis, B. subtilis , Salmonella typhimurium, S. typhimurium and E. coli . , Having an antioxidant activity equivalent to or better than that of a synthetic oxidant, On the basis of this, the present invention has been completed.

Hereinafter, the present invention will be described in detail.

In the present invention, Ecklonia cava is a perennial seaweed with brown algae and versatile seaweed, inhabits deep in the lunch box, and grows up to 1-2 m. The stem is cylindrical, the root is root-shaped, and the middle part of the gown is thick and young when it is young, but it is hollow after it is grown. It is an important algae plant that makes up algae, and is a food for abalone and conch. It is a major raw material for making alginic acid, iodine or potassium, and is sometimes collected and used for food. It is mainly distributed in the south coast of Korea, Jeju Island or Japan.

The extraction solvent of the present invention may be water or an organic solvent such as a polar solvent such as alcohol having 1 to 4 carbon atoms such as methanol or ethanol, ethyl acetate, and a nonpolar solvent of hexane or dichloromethane, or a mixed solvent thereof. Water, alcohols having 1 to 4 carbon atoms, or mixtures thereof, and more preferably water.

Ecklonia cava extract of the present invention may be prepared according to a conventional method for preparing seaweed extract, specifically, may be cold extraction, hot extraction or heat extraction method, for example, may be a hot water extraction method.

In addition, the extract extracted with the solvent may be further fractionated with a solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof. One or more solvents may be used in the fractionation.

The preparation temperature of the extract may be 4 to 150 ℃, preferably 50 to 120 ℃, more preferably 70 to 100 ℃. In addition, the extraction time of the extract is not particularly limited, but may be, for example, 10 minutes to 300 minutes, the extraction method of the extract may use a conventional extraction device, ultrasonic grinding extractor or fractionator.

The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In one embodiment of the present invention, the Ecklonia cava extract is dried and crushed after removing the salt and impurities of Ecklonia cava, to prepare a dry sample, to obtain a crude extract by adding water to the dried sample, the crude extract is concentrated under reduced pressure extract Can be prepared. At this time, the removal of salts and impurities of the Ecklonia cava can be carried out by washing with running water, the drying can be carried out using far-infrared rays, the preparation of the dried sample is dried the Ecklonia remove the salts and impurities It can be prepared after grinding. In addition, the process of obtaining the crude extract by adding the water may be carried out by hot water extraction method using hot water of 50 to 120 ℃.

The obtained Ecklonia cava extract may be stored in a deep freezer until use.

In addition, the Ecklonia cava extract may be one obtained by completely removing the moisture through the concentration and lyophilization of the obtained extract, the Ecklonia cava extract completely removed the moisture is used in the form of powder or dissolved in distilled water or a common solvent Can be.

The present invention provides an antimicrobial composition against microorganisms including the Ecklonia cava extract as an active ingredient. The Ecklonia cava extract may be a crude extract obtained by the extraction solvent or a fraction obtained by the fractional solvent.

In the present invention, "antibacterial" includes not only inhibiting the growth of pathogenic microorganisms such as bacteria or harmful microorganisms, but also inhibiting their sterilization by inhibiting their survival. In addition, in the present invention, "antimicrobial composition" may mean the same as an antibiotic, which is a generic term for an antimicrobial agent, may have the same meaning as an antimicrobial agent, fungicide, preservative, preservative or bactericide, preferably Gram-positive bacteria and It means a substance that can inhibit the development and life function of Gram-negative bacteria and inhibit their survival.

The antimicrobial composition of the present invention may include 0.01 to 99.99% by weight, preferably 0.1 to 99% by weight, based on the total weight of the composition, depending on the method of use and purpose of use of the antimicrobial composition. The content can be adjusted appropriately.

The antimicrobial composition may be an antimicrobial composition having antimicrobial activity against harmful microorganisms or pathogenic microorganisms, specifically Gram-positive bacteria or Gram-negative bacteria, more specifically Staphylococcus aureus , Vibrio sp., Listeria sp., Bacillus sp, Salmonella sp and Escherichia coli ) is an antimicrobial composition having an antimicrobial activity against at least one selected from the group consisting of, preferably Staphylococcus aureus ), enteritis Vibrio ( V. parahemolyticus ), Listeria monocytogenes ( Listeria) monocytogenes ), Bacillus subtilus subtilis, B. subtilis), Salmonella tie blood bunch Titanium (Salmonella. typhimurium, S. typhimurium), and Escherichia coli (an antimicrobial composition having an antimicrobial activity for at least one selected from the group of E. coli), and more preferably Staphylococcus aureus ) and enteritis Vibrio ( Vibrio parahemolyticus , V. parahemolyticus ) is an antimicrobial composition having antimicrobial activity against at least one selected from the group consisting of.

The Staphylococcus aureus is one of the bacteria widely distributed in nature and has been reported as a cause of purulent diseases such as purulent, otitis media or cystitis of the skin, as well as food poisoning, and as a main cause of food poisoning along with Salmonella and enteritis Vibrio. Has been reported. As a relatively heat-resistant bacterium of Staphylococcus aureus, it is known that heat treatment should be performed at 80 ° C. for at least 30 minutes, and enterotoxin produced by Staphylococcus aureus, which is known to cause food poisoning, is 30 at 80 ° C. It is known that even if heat treatment for over a minute does not destroy. Therefore, in contrast to infectious food poisoning, which can be prevented by heat treatment, food poisoning may occur even when ingested foods are treated with toxin-type food poisoning caused by Staphylococcus aureus. When eating foods infected with Staphylococcus aureus, toxins of Staphylococcus aureus may be absorbed into the stomach or intestine, causing food poisoning accompanied by vomiting, diarrhea and abdominal pain.

Since the Staphylococcus aureus can settle in any part of the human body and cause infection, not only infection by contact transmission, but also infection by airborne transmission, in particular, surgery in which the protective mechanism of the skin is damaged. It has been reported that it can settle well in patients or burn patients and cause fatal infections, and in the case of medical workers who have worked for a long time in hospitals, infection by air propagation has been reported to be high because of high intranasal fixation rate.

The Vibrio bacterium is a salt, causing the colitis as well halophilic bacteria grown in the presence salts including Vibrio parahaemolyticus (V. parahaemoliticus) and sepsis parahaemolyticus causing sepsis (V. vulnificus) is typical.

The enteritis Vibrio bacteria are conditionally anaerobic Gram-positive rod bacteria, which are the main causative agents of infectious food poisoning accompanied by diarrhea, abdominal pain, vomiting and fever, which occur when ingesting fish and shellfish by mouth. It is mainly found in seawater, sediment, and invertebrate organisms in coastal and salt estuary, from June to October, and mainly infects aquatic products such as cockle, clams, seaweed, shellfish, shrimp, octopus, fish, and snail. Enteritis vibrio bacteria adhered to the aquatic products can contaminate other foods through cooking utensils such as refrigerators, cutting boards, hangers, knives, and cookers, and food poisoning may be caused by humans eating these contaminated foods.

The Listeria bacterium is mainly a pathogen causing abortion, is isolated from the organs, blood and cerebrospinal fluid of the disease, and can be infected by mammals and birds. Listeria monocytogenes, which causes Listeria disease reported in the academic community among the Listeria bacteria, causes Listeria disease and is a gram-positive aerobic bacterium.

Salmonella is a Gram-negative, anaerobic bacillus, which does not form lactose, does not form indole, and does not produce hydrogen sulfide, which is distinguished from E. coli. Salmonella is a very important food poisoning causative bacterium. Salmonella, a disease caused by Salmonella infection, is classified into sepsis and typhoid, acute gastroenteritis. Food poisoning caused by Salmonella has a higher incidence than food poisoning caused by other bacterial infections, and is accompanied by sudden fever, headache, abdominal pain, diarrhea and vomiting.

The Escherichia coli is a bacterium that lives in the intestines of humans or animals, and may cause cystitis, pyelitis, peritonitis, or sepsis in areas other than the intestine, and infectious diarrhea in the case of antigenic E. coli. In addition, it is related to the cause of corruption of food, etc., it is also used as an indicator of the presence or absence of contamination by feces or indicator bacteria of the water quality test.

The antimicrobial composition of the present invention may be directly applied to animals including humans, and may exhibit antimicrobial activity, and may be applied to marine organisms such as fish or shellfish to lower the mortality rate of fish or shellfish and increase productivity, as well as to marine organisms such as humans. Can prevent secondary infection of other animals that consume The animal is a biological group corresponding to plants and mainly ingests organic matter as nutrients, and means that digestion, embryogenicity, and respiratory organs are differentiated.

The antimicrobial composition of the present invention may include sugars, proteins, lipids, vitamins and minerals in addition to Ecklonia cava extract.

The sugar may be appropriately selected according to the purpose and use thereof, and may be, for example, honey, dextrin, sucrose, palatinose, glucose, fructose, syrup, sugar alcohol, sorbitol, xylitol, maltitol, It is not particularly limited thereto. The protein may be appropriately selected depending on the purpose and use thereof. For example, animal-derived enzymes such as milk derived proteins such as casein and whey protein, soy protein, trypsin of these proteins, and pepsin. And hydrolysates by neutrases and alkalases, but are not particularly limited thereto. The lipid may be appropriately selected according to the purpose and use thereof. For example, the monohydric saturated fatty acid, sunflower oil containing polyunsaturated fatty acid, rapeseed oil, olive oil, safflower oil, corn oil Oil, soybean oil, palm oil (palm oil), palm oil and other plant-derived fats, such as middle-chain fatty acids, EPA, DHA, soybean-derived phospholipids, milk-derived phospholipids, but is not particularly limited thereto. The vitamins are not particularly limited, and the minerals may be appropriately selected depending on the purpose and use thereof. For example, potassium phosphate, potassium carbonate, potassium chloride, sodium chloride, calcium lactate, calcium gluconate, calcium pantothenate, and casein calcium , Magnesium chloride, ferrous sulfate, sodium hydrogen carbonate, but is not particularly limited thereto.

For any of the above saccharides, proteins, lipids, vitamins and minerals, as long as the antimicrobial properties of the composition are maintained, not limited to the above embodiments and may include other ingredients, each content is not limited as long as the antimicrobial properties are maintained Preferably, it may be 0.1 to 15% by weight, preferably 0.5 to 10% by weight relative to the total composition.

In addition, the antimicrobial composition may be formulated in various forms, for example, may be prepared in the form of powders, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, slurries, suspensions or injections. However, the present invention is not limited thereto and may be formulated through various methods known in the art.

The antimicrobial composition of the present invention can be used for various purposes and uses requiring antimicrobial activity, specifically, the composition for the prevention or treatment of diseases caused by pathogenic microbial infection, the composition for animal feed, additives for animal feed, food, food preservatives, It can be used for the use of food additives, beverages, cosmetic compositions, cosmetic additives or detergents.

The present invention also relates to a preservative composition comprising the antimicrobial composition as an active ingredient. Ecklonia cava extract, which is an active ingredient of the antimicrobial composition, not only has antimicrobial activity against microorganisms or bacteria causing food poisoning by causing food decay, but also has a radical scavenging ability similar to that of synthetic antioxidants, thereby oxidizing food. Since it has an inhibitory effect, it has a remarkably excellent food preservation effect as an active ingredient of a preservative composition, especially a food preservative composition.

The preservative composition inhibits or sterilizes the growth of harmful microorganisms or decaying microorganisms such as bacteria, fungi and yeasts, and prevents the deterioration, rancidity or decay of the preservatives that may be infected or decayed by the harmful microorganisms or decayed microorganisms. Means used to maintain the state of the means, preferably means that can be used in one or more selected from the crowd consisting of food, feed, cosmetics and medicines. The composition used for food among the said preservative compositions is called a food preservative composition.

The food preservative composition means a material added to prevent the deterioration, rancidity or decay of the food, the food preservative composition is required to be able to control the changes over time, such as the taste, flavor of the food at the same time to suppress the growth of microorganisms do.

The preservative composition may include 0.01 to 99.9 parts by weight or 0.1 to 50 parts by weight of the antimicrobial composition based on 100 parts by weight of the total composition.

In addition, in addition to the antimicrobial composition comprising the antimicrobial composition, it may further include a conventional material included in the preservative composition. In addition, the preservative composition may be used with a conventional synthetic preservative or an organic acid mixture, within the range that the preservative composition does not limit the effects of the present invention. In addition, when the preservative composition is a food preservative composition, a food acceptable solvent or diluent may be further added for the purpose of increasing the preservation of foodstuffs.

When the preservative composition is a food preservative composition, the organic acid may be lactic acid, acetic acid, citric acid, malic acid, and the like which can be used in food. For example, the organic acid may be lactic acid having a high antibacterial activity and no irritating taste or smell. In addition, the salts of the organic acid may be used, and in the case of the salts of the organic acid, the base salts of the organic acid may have a buffering effect, thereby reducing the pH change due to the external environment of the antimicrobial composition.

The food preservative composition can be used in various foods. The food preservative composition may be used in processed foods or packaged foods, such as bread, rice cake, instant soup, such as natural foods such as fish, meat and vegetables, such as noodles, raw noodles, etc., such as fish, meat products, processed foods , Fish meat products, tofu, jelly, health supplements, seasonings, bakery and confectionery, dairy products, pickles, fermented foods or beverages, but is not limited to these types.

The food preservative composition may be added directly to the manufacturing process of the food, the method of addition may be, for example, a method of immersing the food in the composition or spraying the composition on the food. Method of treating the food preservative composition by immersing the food is 0.5 to 10.0% by weight, preferably 1 to 7.5% by weight, more preferably 2 to 5% by weight based on the concentration of the Ecklonia cava extract It may include the step of immersing in the immersion liquid containing a preservative composition, or soaking for 30 seconds to 30 minutes, the concentration and immersion time of the immersion liquid may be appropriately adjusted according to the type of food. In the case of processed meat or processed food, the food preservative composition may be treated by adding the food preservative composition suitable amount of the present invention to the food at the beginning of processing, and in the case of unprocessed food such as packaged meat, it is applied to the outer surface of the food. The food preservative composition can be treated by the method.

The amount of the food preservative composition can be adjusted according to the characteristics of the food to be processed. For example, in the case of foods having a small amount of fat such as confectionery, the preservation effect can be enhanced by only a small amount, and thus only a small amount can be processed. Therefore, the ratio to be added to food is not particularly limited, and an appropriate amount can be selected within the range of maintaining the characteristics or taste of the food, and processed seafood, processed food, fish meat, seasoned food, bakery and confectionary food, By using the food preservative composition in an appropriate amount for all kinds of products including dairy products or fermented foods, not only the preservation of food can be improved, but also the inherent taste of food can be maintained.

In addition, the present invention provides a food preservation method comprising the step of treating food with the food preservative composition.

The processing of the food may be performed by immersing the food in an immersion liquid containing the food preservative composition, adding the food preservative composition to the food, or spraying the food preservative composition on the food. The method of treating the food preservative composition by immersing the food in an immersion liquid containing the food preservative composition is 0.5 to 10.0% by weight, preferably 1 to 7.5% by weight, more preferably 2 to 2, based on the concentration of the Ecklonia cava extract. The food may be washed in an immersion liquid containing the food preservative composition prepared to be 5% by weight, or immersed for 30 seconds to 30 minutes. For example, during the manufacturing process of the fishery processing product described in FIG. 1, the food preservative composition may be added to the washing solution of the second washing step to be immersed or washed.

In addition, the present invention provides a composition for treating or preventing food poisoning comprising the antimicrobial composition as an active ingredient.

The food poisoning is an acute or chronic disease caused by the ingestion of food containing natural poisons or harmful substances, mainly accompanied by symptoms such as fever, vomiting, diarrhea and abdominal pain. The food poisoning may be classified into bacterial food poisoning, chemical food poisoning, natural poisoning food poisoning and food poisoning by microbial toxic metabolites, and the bacterial food poisoning may be classified into infectious food poisoning and toxin type food poisoning.

Most of the food poisoning is bacterial food poisoning caused by bacteria, the most common is the food poisoning caused by infectious food poisoning caused by Salmonella, enteritis Vibrio or Escherichia coli, and toxins produced by Staphylococcus aureus or Boldulinus.

The composition for the prevention or treatment of diseases caused by food poisoning infection of the present invention may further comprise a carrier or excipient that can be used pharmacologically in addition to the Ecklonia cava extract according to the formulation and method of use, the composition of the present invention alone or to It can be administered in combination with other pharmaceutically active ingredients to enhance efficacy as a therapeutic agent for infection by. In this case, the content of the Ecklonia cava extract in the composition for preventing or treating food poisoning may be 0.001 to 99% by weight. If the content of the active ingredient in the composition is less than 0.001% by weight may require a large amount of administration for effective efficacy, if the amount exceeds 99% by weight may be constant compared to the amount may be uneconomical. In addition, the composition for the prevention or treatment of diseases caused by food poisoning according to the method of use and purpose of use can appropriately adjust the content of the Ecklonia cava extract.

The composition for preventing or treating food poisoning of the present invention may be administered orally or parenterally, preferably orally, but the formulation thereof may vary depending on the method of use, and is not limited thereto. Examples of formulations include PLASTERS, GRANULES, LOTIONS, POWDERS, SYRUPS, LIQUIDS AND SOLUTIONS, AEROSOLS, OINTMENTS, FLOWERS FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, INFUSIONS, TABLETS, INJECTIONS, CAPSULES and PILLS.

Furthermore, the composition for preventing or treating food poisoning of the present invention may be prepared by using a method known in the art or a method disclosed in Remington's Pharmaceutical Science (Recentington, Mack Publishing Company, Easton PA). And preferably formulated.

Excipients such as pharmacologically acceptable carriers or diluents, preservatives, stabilizers, wetting agents, emulsifiers, solubilizers, sweeteners, colorants, osmotic pressure regulators, antioxidants, etc. may use conventional materials according to the formulation, and when formulated, fillers , Extenders, wetting agents, disintegrating agents or surfactants may be used. Representative diluents or excipients include water, dextrin, calcium carbonate, lactose, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyloleate, liquid paratin and saline.

Specifically, in the case of oral administration depending on the formulation, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. which are commonly used can be used, and in the case of parenteral administration, sterilized aqueous solution, non-aqueous solvent Suspensions, emulsions, lyophilized preparations, suppositories, and the like can be used. The non-aqueous solvent or suspending agent may be, for example, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like, and suppositories may include, for example, utopsol, macrogol, and tween. 61, cacao butter, laurin butter or glycerogelatin and the like.

In addition, the composition for preventing or treating food poisoning of the present invention may be used in admixture with various carriers acceptable as a medicament such as physiological saline or organic solvent, and in order to increase the stability or absorbency, glucose, sucrose or dextran It can be used in combination with such carbohydrates, ascorbic acid or antioxidants such as glutathione, chelating agents, small molecule proteins or other stabilizers.

The dosage or amount of the composition for preventing or treating food poisoning of the present invention is preferably determined in consideration of the weight, age, sex, health status, diet, time of administration, administration method, excretion rate and severity of the disease of the patient. For example, the effective daily dose is typically 10 to 5000 mg / kg and preferably 100 to 3000 mg / kg for adults (70 kg) and can be administered 1 to several times a day, preferably 1 per day. To three times.

Since the dosage may vary depending on various conditions, it will be apparent to those skilled in the art that the dosage may be added or subtracted, and thus the dosage does not limit the scope of the present invention in any aspect. The frequency of administration can be administered once a day or divided into several times within the desired range, the administration period is not particularly limited. In addition, the composition for preventing or treating food poisoning of the present invention, in addition to oral administration as it is, may be added to any food and consumed daily.

The composition for preventing or treating food poisoning of the present invention not only provides an effective food poisoning bacterium, specifically, the prevention or treatment of infection by the antibacterial effect against Staphylococcus aureus, enteritis Vibrio, Salmonella typhimurium and Escherichia coli, It is a natural seaweed, because it contains Ecklonia cava extract as an active ingredient can be taken for a long time.

The present invention also provides a food composition for preventing or improving food poisoning comprising the antimicrobial composition as an active ingredient. The food composition may preferably be a functional food composition.

In the present specification, the term "food" means a natural product or processed product containing one or more nutrients, and preferably means a state in which it can be directly eaten through some processing process. It is intended to include all foods, food additives, functional foods and beverages.

Examples of the food to which the antimicrobial composition of the present invention can be added include various foods, beverages, gums, teas, vitamin complexes, and functional foods. In addition, the food in the present invention includes special nutritional products (e.g., prepared oils, infants, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g. ramen, noodles, etc.), health supplements, seasoned foods ( For example, soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.), beverages ( Examples include, but are not limited to, fruits, vegetable drinks, soy milk, fermented beverages, and the like, and natural seasonings (eg, ramen soup). The food, beverage or food additives may be prepared by a conventional manufacturing method.

Functional food in the present invention is the control of biological defense rhythm, disease prevention and recovery of food groups or food compositions that have added value to the food by using physical, biochemical, biotechnological techniques, etc. It means a food processed and designed to fully express the gymnastics function related to the living body. The functional food may include food acceptable food additives, and may further include appropriate carriers, excipients and diluents commonly used in the manufacture of functional foods.

In addition, the food for the prevention or improvement of food poisoning containing the antimicrobial composition as an active ingredient, a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavoring agents, such as colorants and fillers (cheese, chocolate, etc.) ), Pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages and the like. These components can be used independently or in combination. The proportion of such additives is not so critical, but may be selected in the range of 0.01 to 20 parts by weight per 100 parts by weight of Ecklonia cava extract.

In addition, in the food for preventing or improving food poisoning comprising the antimicrobial composition as an active ingredient, the amount of the Ecklonia cava extract may include 0.01 to 15% by weight of the total food weight, the beverage composition is 0.02 based on 100 ml To 5 g, preferably 0.3 to 1 g.

The present invention also provides a composition for animal feed comprising the antimicrobial composition as an active ingredient.

The animal is a biological group corresponding to plants, mainly ingesting organic matter as nutrients, and means that digestion, excretion and respiratory organs are differentiated, specifically, echinoderm, crustaceans, molluscs, fish, amphibians, reptiles, birds, It may be a mammal, preferably fish, crustacean arthropods, echinoderm, mollusks, birds or mammals including poultry.

The animal feed composition may further include grain, vegetable protein feed, animal protein feed, sugar or dairy product in the antimicrobial composition comprising the Ecklonia cava extract as an active ingredient. The grains may be specifically milled or crushed wheat, oats, barley, corn and rice, and the vegetable protein feed may be specifically based on rapeseed, soybean and sunflower, the animal protein feed specifically May be blood meal, meat meal, bone meal and fish meal, and the sugar or dairy product may specifically be a dry ingredient consisting of various powdered milk and whey powder.

The animal feed composition may further be used with ingredients such as nutritional supplements, digestion and absorption enhancers, sex stimulants or disease prevention agents.

The antimicrobial composition included in the composition for animal feed of the present invention may vary depending on the purpose and conditions of use of the feed. For example, the antimicrobial composition may be included in an amount of 0.1g to 100g based on 1kg of the final produced feed.

In addition, the feed composition may be made of a viscous granulated or granular material depending on the degree of grinding of the components, the composition may be supplied in a mesh or formed into a desired separated shape for further processing and packaging. It may be subjected to a pelletizing, expanding or extruding process for storage, and preferably excess water may be dried off for ease of storage.

The present invention also provides a cleaning agent containing the antimicrobial composition as an active ingredient. The detergent may be preferably a detergent for clothes, a dish cleaner, a food cleaner, a household appliance cleaner. Since the antimicrobial composition is not harmful to the human body as a natural ingredient, more preferably, it may be used for the purpose of washing or providing antimicrobial properties of various household goods including kitchenware or food washing.

The cleaning agent may further include an appropriately selected additive according to the purpose of use and use thereof, and may be mixed with other active ingredients such as a commonly used cleaning agent, and additives such as colorants, surfactants, and preservatives. The detergent may be prepared by powdering, granulating, tableting or liquefying according to the purpose and use thereof, and a conventional method may be used for packaging for commercialization.

In addition, the amount of the antimicrobial composition used in the cleaning agent may be suitably used according to the purpose of use, application form, application site, object to be applied, and the like, and may include, for example, 0.01 to 50 parts by weight based on the total composition. The content is not limited thereto. Since the antimicrobial composition of the present invention is a natural ingredient, which is not harmful to the human body, it can be used in various amounts as long as it can achieve an application purpose due to the characteristics of the product.

Ecklonia of the present invention The antimicrobial composition containing cava ) extract as an active ingredient has excellent antimicrobial activity against microorganisms that cause diseases such as food decay and food poisoning, and has free radical scavenging ability similar to or better than that of synthetic antioxidants, that is, antioxidant activity. Since it can prevent the infection of harmful microorganisms on food, kill the infected microorganisms, prevent the oxidation of foods, and use them for food, unlike conventional antimicrobial or food preservatives. As it contains the extract extracted from Ecklonia cava, which is the algae, as an active ingredient, side effects are not a problem, and it is economically advantageous because it uses abundant algae on the southern coast of Korea including Jeju Island. Can be.

Hereinafter, preferred embodiments of the present invention are described. However, the following examples are only preferred embodiments of the present invention, and the present invention is not limited to the following examples.

Example 1: Preparation of Ecklonia cava Extract

The Ecklonia cava used in the test was collected from offshore of Jeju Island, and the collected Ecklonia cava was removed by attaching it, washed with clean fresh water, completely dried using far-infrared rays, and then ground using a grinding machine. To 30 g of the crushed Ecklonia cava flour was added 500 ml of hot water at 100 ° C. to prepare an extract by hot water extraction. The extract was filtered, concentrated under reduced pressure using a reduced pressure concentrator, and lyophilized to prepare a powder.

The obtained Ecklonia cava extract was stored at -20 ° C in a deep freezer until use. The total amount of water extract obtained from 30 g of the Ecklonia cava flour was 10 g.

Example 2: Determination of antimicrobial activity of Ecklonia cava extract

For the crude extract of Ecklonia cava prepared in Example 1, antimicrobial activity against six representative harmful microorganisms related to food spoilage was measured using a paper disk method.

2-1: Target strain and culture medium

Strains used for the determination of antimicrobial activity were each published strains distributed from the corresponding deposit institution. Specifically, the deposit number of the strain measuring the antimicrobial activity is as follows.

The accession number of Bacillus subtilis is KCCM 40820, the accession number of Staphylococcus aureus is KCTC 1927, the accession number of Escherichia coli is KCCM 40880, the accession number of Salmonella typhimurium is KCCM 40253, and the deposit of enteritis vibrio The number is KCCM 11965 and the accession number of Listeria monocytogenes is KCCM 40307.

Muller-Hinton broth (MHB) medium used for the cultivation of the strain and Muller-Hinton agar medium, a medium used for the paper disk method, were purchased from Difco (USA). In addition, 8 mm paper disc for antibacterial measurement was purchased from ADVANTEC (Japan).

2-2: Antibacterial Activity Measurement Using Paper Disk

The antimicrobial activity against the harmful microorganisms was measured by a plate medium diffusion method using a paper disk containing the Ecklonia cava extract prepared in Example 1 as an antimicrobial target material.

More specifically, the cultured strains of Example 2-1 pre-cultured in Muller Hinton broth (Difco, USA) medium for 12 hours to culture the turbidity of the culture MacFarland No. After adjusting to 0.5, the turbidity-adjusted culture was evenly spread over the front of Muller Hinton agar (Difco, USA) medium using a sterile swab.

The Ecklonia cava extract prepared in Example 1 was dissolved in distilled water according to each concentration, and then absorbed into a paper disc (Advantec Filter Paper, Toyo Roshi Kaisha Ltd, Japan) having a diameter of 8 mm and a thickness of 1.5 mm and dried to completely remove the solvent. After the evaporation, the test strains were attached to the agar medium, and the test strains were incubated for 24 hours at the optimum culture temperature for each test strain, and then the size of the produced inhibitory ring was measured to confirm the antibacterial effect. The results are shown in FIGS. 2 and 3 and Table 1 below. The diameter of the inhibitory ring of the following table is the size including the paper disc, '-' means no growth inhibition ability of the strain, '+' means that the size of the inhibitory ring is 8 to 12mm, '++' is It means that the size of the inhibitory ring is 12 to 15mm, '+++' means that the size of the inhibitory ring is 15 to 20mm, '++++' means that the size of the inhibitory ring is 20 to 25mm, each The size of the inhibitor ring is a measure of the overall diameter of the inhibitor ring, including the paper disc.

TABLE 1

concentration (mg / ml) One 2 3 5 Escherichia coli + + + ++ Salmonella typhimurium - + ++ ++ Staphylococcus aureus +++ +++ ++++ ++++ Listeria monocytogenes + ++ ++ ++ Bacillus subtilis - - + + Vibrio parahaemolyticus ++ +++ +++ +++

As a result of measuring the antimicrobial activity, as shown in Table 1 and FIGS. 2 and 3, it was confirmed that the Ecklonia cava extract has antimicrobial activity against six kinds of microorganisms, which are representative harmful microorganisms related to food rot or food poisoning. In particular, it showed excellent antimicrobial activity against Staphylococcus aureus and Vibrio parahaemolyticus , which are the main causes of food rot and food poisoning, even when treated at the lowest concentration of 1mg / ml. It showed antimicrobial activity against Listeria monocytogenes , the causative agent of Listeria disease, and E. coli , a marker of food decay and food contamination. The antimicrobial activity of Salmonella typhimurium and Bacillus subtilis , which are food decay microorganisms, has been shown to have antimicrobial activity. The trend was shown.

Putting together the results showing the antimicrobial activity against the harmful microorganisms of Ecklonia cava hot water extract measured by the Agar diffusion method, Ecklonia cava extract can be used as a food preservative to suppress the reproduction of food spoilage microorganisms In addition, it can be used in the composition for the treatment, prevention or improvement of food poisoning using antibacterial activity against Staphylococcus aureus and enteritis Vibrio bacteria, which cause food poisoning, and to prevent this, cleaning compositions or animals other than humans, especially livestock or It is expected to be used as a feed composition for fish processed fish and aquaculture industry.

In particular, since the Ecklonia cava is obtained from natural plants used as food ingredients, it does not cause any problems such as side effects of existing antibacterial agents or compounds, and it is economically beneficial because it can be easily obtained from the south coast of Korea including Jeju. By the way, the antibacterial composition using Ecklonia cava can be said to have a great industrial value.

Example  3: Ecklonia  Antioxidant Activity of Extracts

For the crude extract of Ecklonia cava prepared in Example 1, the antioxidant activity was confirmed by measuring DPPH free radical scavenging activity, Hydroxyl radical scavenging activity, Hydrogen peroxide scavenging activity and Alkyl radical scavenging activity.

3-1: DPPH Law free radical  Scavenging activity measurement

DPPH free radical scavenging activity of Ecklonia cava extracts was measured by an Electron-Spin Resonance Spectrometer (ESR) by Nanjo et al.

More specifically, 60 μl of 60 μM DPPH solution was added to 60 μl of the sample prepared according to each concentration, followed by stirring for 10 seconds, and then the mixed solution was transferred to a quartz capillary tube. After the passage, the spectrum was measured with an electron spin resonance absorption spectrometer. Spectra were recorded under the conditions of scan time: 30s, filed: 336 ± 5mT, time constant: 0.3s, power: 5mW, and amplitude: 1 × 500. 1 mg / ml of BHT was used as a comparative example for the scavenging activity. DPPH radical scavenging activity of the antioxidant sample using the measured spectrum was calculated using the following formula 1.

[Calculation 1]

The value measured by the above formula 1 is shown in FIG. As shown in FIG. 4, the free radical scavenging activity of the Ecklonia cava extract exhibited a high scavenging activity of about 81% when 0.5 mg / ml was used, which was superior to that of BHT (butylhydroxytoluene) having an antioxidant activity as a synthetic antioxidant. It was confirmed that. In addition, even when using a small amount of 0.5 mg / ml exhibited excellent radical scavenging ability, it was confirmed that the Ecklonia cava extract has excellent antioxidant activity.

3-2: Hydroxyl radical  Scavenging activity measurement

The hydroxyl radical scavenging activity of Ecklonia cava extract was measured by Rosen et al.

More specifically, 20 μl of the sample added to each concentration of the extract prepared in Example 1 and 20 μl of 0.3 M DMPO, 10 μm FeSO ₄ 20 μl, 10 μM H ₂ O ₂ 20 μl were mixed, and the stirred mixed solution was quartz capillary. After 2 minutes and 30 seconds after the transfer to the tube, the spectrum was measured by an electron spin resonance absorption spectrometer. Spectra were recorded under the conditions of scan time: 30s, filed: 336 ± 5mT, time constant: 0.3s, power: 1mW, and amplitude: 1 × 100. 2 mg / ml of BHT was used as a comparative example for the scavenging activity. DPPH radical scavenging activity of the antioxidant sample using the measured spectrum was calculated using the following formula (2).

[Calculation 2]

The value measured by Formula 2 is shown in FIG. 5. As shown in FIG. 5, the hydroxyl radical scavenging activity of the Ecklonia cava extract was confirmed to increase with increasing concentration of the extract. In particular, even when 1mg / ml corresponding to 20% of the amount of BHT was used, it exhibited a high scavenging ability of about 80%, it was confirmed to have a superior antioxidant activity than the synthetic antioxidant BHT.

3-3: Hydrogen peroxide  Scavenging activity measurement

Hydrogen peroxide scavenging activity of Ecklonia cava extract was measured by M method.

More specifically, 100 μl of sample and 0.1 M phosphate buffer (pH 5.0) added to each concentration of the extract prepared in Example 1 were mixed in a 96 microwell plate. After adding 20 μl of hydrogen peroxide to the mixed solution, the reaction was performed at 37 ° C. for 5 minutes, and 30 μL of 1.25 mM ABTS and peroxidase (1 unit / mL) were added to the reaction solution, and the reaction solution was reacted at 37 ° C. at 37 ° C. After further reaction for 10 minutes, it was carried out by measuring the absorbance at 405nm. 2 mg / ml of BHT was used as a comparative example for the scavenging activity. DHyerogen peroxide scavenging activity of the antioxidant sample using the measured absorbance was calculated using the following equation (3).

[Calculation 3]

The value measured by the above Equation 3 is shown in FIG. As shown in FIG. 6, the free radical scavenging activity of the Ecklonia cava extract exhibited a high scavenging activity of about 81% when 0.5 mg / ml was used, which is superior to that of BHT (butylhydroxytoluene) whose antioxidant activity was confirmed as a synthetic antioxidant. Confirmed. In addition, even when using a small amount of 0.5 mg / ml exhibited excellent radical scavenging ability, it was confirmed that the Ecklonia cava extract has excellent antioxidant activity.

3-4: Alkyl radical  Scavenging activity measurement

Alkyl radical scavenging activity of Ecklonia cava extract was measured by Hiramoto et al.

More specifically, 20 μl of the sample added to each concentration of the extract prepared in Example 1 and DW 20μl, 40 mM AAPH 20μl, 40 mM POBN 20μl and the stirred mixed solution was reacted for 30 minutes at 37 ℃ After that, the spectra were transferred to a quartz capillary tube with an electron spin resonance absorption spectrometer. Spectra were recorded under the conditions of scan time: 30s, filed: 336 ± 5mT, time constant: 0.3s, power: 7mW, and amplitude: 1x500. 1 mg / ml of BHT was used as a comparative example for the scavenging activity. Alkyl radical scavenging activity of the antioxidant sample using the measured spectrum was calculated using the following equation (4).

[Calculation 4]

The value measured by the above formula 4 is shown in FIG. As shown in FIG. 7, the free radical scavenging activity of the Ecklonia cava extract was found to be about 81% higher when using 0.5 mg / ml, and compared to BHT (butylhydroxytoluene), which has been identified as a synthetic antioxidant. It was confirmed to be excellent. In addition, even when using a small amount of 0.5 mg / ml exhibited excellent radical scavenging ability, it was confirmed that the Ecklonia cava extract has excellent antioxidant activity.

In summary, the results of measuring the antioxidant activity, Ecklonia cava extract was found to have a superior antioxidant activity than the BHT confirmed as an antioxidant in the scavenging activity for DPPH free radical, Hydroxyl radical, Hydrogen peroxide and Alkyl radical. .

From the above results, the Ecklonia cava extract can inhibit the oxidation of food, can be used as a food antioxidant, the Ecklonia cava extract which is recognized the antimicrobial activity and the antioxidant activity against the food spoilage microorganism is a food preservation effect Is not only evaluated as excellent, but also obtained from a natural plant that can be used as a food material does not cause problems such as side effects of existing antimicrobial agents and compounds, and using the Ecklonia cava, which can be easily obtained in the south coast of Jeju Island, etc. Since it is economically beneficial, an antimicrobial composition comprising the Ecklonia cava extract as an active ingredient or a food preservative comprising the same as an active ingredient is considered to have a great industrial value.

1 is a flowchart illustrating a fishery processed product manufacturing process according to an embodiment of the present invention.

Figure 2 is a photograph of the antimicrobial activity against Staphylococcus aureus according to the amount of Ecklonia cava hot water extract according to an embodiment of the present invention measured by a plate medium diffusion method using a paper disk. Counterclockwise from top left, with 1 mg, 2 mg, 3 mg, and 5 mg Ecklonia hydrothermal extract.

Figure 3 is a photograph of the antimicrobial activity against enteritis Vibrio bacteria according to the amount of Ecklonia cava hot water extract according to an embodiment of the present invention measured by a plate medium diffusion method using a paper disk. Counterclockwise from top left, with 1 mg, 2 mg, 3 mg, and 5 mg Ecklonia hydrothermal extract.

Figure 4 is a graph confirming the DPPH free radical scavenging ability according to the content of Ecklonia cava hot water extract according to an embodiment of the present invention. The numerical values set forth below refer to the concentrations of Ecklonia cava extract.

Figure 5 is a graph confirming the hydroxyl radical scavenging ability according to the content of Ecklonia cava hot water extract according to an embodiment of the present invention. The numerical values set forth below refer to the concentrations of Ecklonia cava extract.

Figure 6 is a graph confirming the hydrogen peroxide radical scavenging ability according to the content of Ecklonia cava hot water extract according to an embodiment of the present invention. The numerical values set forth below refer to the concentrations of Ecklonia cava extract.

Figure 7 is a graph confirming the alkyl radical scavenging ability according to the content of Ecklonia cava hot water extract according to an embodiment of the present invention. The numerical values set forth below refer to the concentrations of Ecklonia cava extract.

Claims (11)

Ecklonia cava ) Antimicrobial composition having antimicrobial activity against microorganisms, including the extract as an active ingredient. The antimicrobial composition of claim 1, wherein the Ecklonia cava extract is extracted using at least one selected from the group consisting of water, alcohol having 1 to 4 carbon atoms, ethyl acetate, hexane, and dichloromethane. The antimicrobial composition of claim 2, wherein the extractant is water, an alcohol having 1 to 4 carbon atoms, or a mixture thereof. According to claim 1, wherein the harmful microorganisms are Staphylococcus aureus , Vibrio sp., Listeria sp., Bacillus sp., Salmonella sp. Escherichia coli ( Esherichia coli ) is one or more antimicrobial composition selected from the group consisting of. The antimicrobial composition of claim 4, wherein the harmful microorganism is at least one selected from the group consisting of Staphylococcus aureus and Vibrio parahemolyticus . The antimicrobial composition of claim 1, wherein the antimicrobial composition has an antioxidant activity. A food preservative comprising the antimicrobial composition of any one of claims 1 to 6 as an active ingredient. 8. The food preservative of claim 7, wherein the food preservative is for addition to aquatic products or processed fish products. A food poisoning treatment or prevention composition comprising the antimicrobial composition of any one of claims 1 to 6 as an active ingredient. A food composition for preventing or improving food poisoning comprising the antimicrobial composition of any one of claims 1 to 6 as an active ingredient. A cleaning composition comprising the antimicrobial composition of any one of claims 1 to 6 as an active ingredient.

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