KR101749525B1 - Disposable absorbent articles comprising deodorizing composition having antimicrobial and antiinflammatory activity - Google Patents

Abstract

The present invention relates to a method for preventing and treating bad breath, such as oral diseases and oral microorganisms, including the effective ingredient of plum extract and porcine potato extract, by maximizing the deodorizing effect by increasing the active ingredient content by fermenting the menthol, The present invention relates to a composition for suppressing and eliminating odor-inducing substances, such as odor-inducing substances, such as odor-inducing substances. The cytotoxicity and single-toxicity tests have confirmed that the composition has little toxicity and excellent deodorizing effect, Can be applied and manufactured as a disposable composition and a disposable absorbent product containing the same.

Description

Disposable absorbent articles comprising a deodorant composition having antimicrobial, antiinflammatory activity (disposable absorbent composition comprising antimicrobial and antiinflammatory activity)

The present invention relates to a disposable absorbent product comprising a deodorant composition having antibacterial and antiinflammatory activity, and more particularly to a disposable absorbent product containing an extract of a natural plant, that is, a fermented mash extract, as an active ingredient and further comprising a plum extract and a potato extract And a deodorant composition having antibacterial and antiinflammatory activity against Streptococcus aureus and Escherichia coli as a skin uptake bacteria, and a disposable absorbent product comprising a deodorant composition containing the deodorant composition, will be.

The types of odors derived from the human body include many odors including bad breath and moles.

Bad breath is not only the odor caused by the oral cavity of the human body, but also the unpleasant odor coming from the oral cavity through the stomach, liver, lungs and other systemic organs other than oral cavity. As described above, the bad breath is composed of the volatile sulfur compounds generated because the enzyme in the oral cavity is decomposed using the epithelial connective tissue, plant residue, or oral bacteria existing in the saliva as a protein source. Among them, methyl mercaptan, hydrogen sulfide, dimethyl sulfide and the like are known, and methylmercaptane is known as the most unpleasant odor of halitosis.

Depending on the causes of bad breath, oral causes account for 85% to 90% of oral causes and oral causes. Bad breath due to oral causes is a result of host factors such as tooth and saliva components and food residues being corrupted by bacteria, which may include saliva secretion reduction, stomatitis, periodontal disease, improper prosthesis, And excessive microbial deposits. Smoking, drinking, and other causes such as decreased amount of saliva, increase in saliva viscosities, oral cavity microorganisms and tooth decay are the biggest causes.

It is known that about 300 kinds of microorganisms reside in the surface of the teeth, between the tooth and gums of the root portion, and on the surface of the tongue, and the presence of such microorganisms is a normal phenomenon when proper oral hygiene activities are performed. However, pathogenic microorganisms can cause oral diseases such as tooth decay, gingivitis and periodontal disease. Streptococcus mutans ( Streptococucus) The microorganisms such as mutans and Streptococcus sobrinus attach to the surface of the teeth and form a bacterial community called plaque after a few hours. In the early stage, pathogenic microorganisms are concentrated on the tooth surface It forms a plaque, but as it progresses, plaque is formed on the surface of the tooth under the gingiva. When the plaque is formed on the tooth surface, the microorganisms produce acid using the sugar introduced into the oral cavity, and this acid causes destruction of the tooth by demineralizing the enamel of the tooth, causing tooth decay. On the other hand, germs in the subgingival gut, especially anaerobic oral microorganisms, secrete toxins and proteolytic enzymes to directly destroy the periodontal tissues or react with immune cells of our body to induce the production of various immune substances, It can lead to inflammation and destruction of tissue. Through this process, when tooth decay and periodontal disease occur, food waste is adhered to the area, which further exacerbates the bad breath.

Therefore, it is very urgent to develop a component having a double effect by eliminating root cause and preventing bad breath by preventing tooth decay and periodontal disease which cause bad breath.

As the number of consumers who use 'mouthwash cleaner' which is easy to carry and do not brush easily or feel the cooling of mouth is increasing, the market size of related products is also growing steadily. In recent years, as a result of various products with enhanced efficacy and effects such as cavity, gum disease prevention, and plaque removal, consumer interest in related products is increasing. However, most oral antibiotics include chlorohexidine or cetyl pyridinium chloride. However, these antimicrobial agents are difficult to use for a long period of time because they may cause the appearance of resistant bacteria and the bacterial exchange. In particular, when chlorhexidine is used in the oral cavity at a high concentration, or when it is used at a low concentration for a long time, side effects may occur such as cohesion of the tongue and teeth, peeling of the oral mucosa, Menthol, peppermint, and other fragrances are also used in many cases. These are basically the masking effect that temporarily covers the bad breath with a strong fragrance of fragrance rather than removing the bad breath. The persistence of the effect It is insignificant. It is an urgent matter to develop a composition for the prevention or treatment of oral diseases derived from natural substances which can be ingested orally and which does not cause side effects even if it is used for a long period of time.

In addition to improving dietary habits, intake of high-calorie foods such as high-fat foods and instant foods has increased, while constipation is caused by lack of fiber intake and exercise. Constipation is defined as constipation when the stool is difficult or frequency is rare, and the stomach is more than normal. When the stool is less than 30 grams per day, or when the stool frequency is less than 2 times per week, constipation can be diagnosed. These constipations occur when lack of water intake, intake of food, especially low intake of fiber, insufficient exercise, emotional instability, and frequent stasis. In addition, sitting and working all day, accumulation of occupation or stress decreases the movement of the stomach and the ability to move the stomach is often constipated. As constipation continues for a long time, the occupation rate of harmful bacteria in the large intestine increases, so that the protein is used as a food source to produce a large amount of ammonia gas, and the time for staying in the stomach is lengthened, .

Among the deodorized materials reported in the past, there are few substances exhibiting a deodorizing effect with respect to monosulfide compounds such as allylmethyl monosulfide. Also in the case of green tea extract (Japanese Patent No. 1330998) which shows a high effect on the deodorization of thiol compounds, It is known that the deodorizing effect on methyl monosulfide is insufficient. Since the monosulfide compound such as allylmethyl monosulfide has a deodorization mechanism greatly different from the thiol compound such as allyl mercaptan and methyl mercaptan, even a deodorant having a deodorizing effect on the thiol compound is not effective for the monosulfide compound There are many.

It is known that benzaldehyde and cinnamaldehyde react with monosulphide to exhibit a deodorizing effect as a component derived from a natural product. However, since these aldehydes have a strong strong odor and their practical use is limited, practical utility for monosulphide compounds Development of high and highly safe deodorant is required.

As a deodorant component derived from natural products, rosemary and raspberry plants have been found to have a deodorizing effect on methyl mercaptan, which is a thiol compound, and trimethylamine, which is a nitrogen compound (Japanese Patent Publication No. 1823565) The composition for incineration is also disclosed as a deodorizing material for methyl mercaptan (Japanese Patent Application Laid-Open No. 05-269187). However, the deodorizing effect of allylmethyl monosulfide, which is a monosulfide compound, has not been revealed in both of the above cases.

The extract obtained from eucalyptus has also been disclosed as a deodorant for hydrogen sulfide, ammonia, amines, mercaptans and nicotine (JP 60-261458 A), but the deodorizing effect of allylmethyl monosulfide, which is a monosulfide compound, none.

In the case of a rare tree, the effect of air purifying agent on decaying meat pieces and deodorization in an animal breeding room is known (Japanese Patent Application Laid-Open No. 51-27882). However, these odors are generally caused by decomposition of hydrogen sulfide, Nitrogen compounds such as ammonia and amines, and lower fatty acids are the main causative substances, and the deodorizing effect on allylmethyl monosulfide is not known.

The extract obtained from Hamamelis has been disclosed as a deodorant for the side odor, foot odor, body odor and human odor of the human body (Japanese Patent Application Laid-Open No. 2000-186025). However, the above odor component contains lower fatty acids including isovaleric acid, Aldehydes are predominant, and there is no report that allylmethyl monosulfide is contained. Therefore, the deodorizing effect on the allylmethyl monosulfide by the component derived from Hamamelis is not known.

Other odor originated from other human body may cause a lot of other odors such as urine odor, physiological odor, etc. Especially, urine odor and physiological odor are not covered by disposable absorbent products, and disposable absorbent products are appropriately replaced in a timely manner If not used, the smell becomes worse and the skin is adhered to the skin, which may adversely affect the skin.

Therefore, it is sufficient to develop a deodorizing effect against the odor originating from the human body including at home and abroad, and a product that sufficiently exhibits antibacterial and anti-inflammatory activity against Streptococcus aureus and Escherichia coli There is no way.

It is an object of the present invention to provide a disposable absorbent product comprising a deodorant composition having excellent antibacterial and anti-inflammatory activity by producing a high content of polyphenol by fermenting and extracting microbes from a menthol.

Another object of the present invention is to provide a disposable absorbent product comprising a fermented mushroom extract as an active ingredient, further comprising a mushroom extract and a potato extract as an active ingredient, .

Disposable absorbent products comprising a deodorant composition having an antibacterial and anti-inflammatory activity according to the present invention include an absorbent pad which adheres to a human body and absorbs secretions, wherein the absorbent pad is a deodorant composition .

The disposable absorbent article includes at least one member selected from the group consisting of a superabsorbent resin, a liquid-permeable upper sheet, a liquid-impermeable lower sheet, a leakage preventing band, and a padding band for tightly fixing and fixing the absorbent pad to a human body, As shown in FIG.

The deodorant composition may comprise a solvent as a residual amount of 10 to 50% by weight of the fermented roots extract.

The fermented mushroom extract may be obtained by fermenting mushroom and extracting the mushroom fermentation product obtained by alcohol extraction.

The attritive fermented product may be fermented with yeast.

The deodorant composition may further comprise a plum extract.

The deodorant composition may further comprise 1 to 20% by weight of the plum extract based on the total weight of the deodorant composition.

The deodorant composition may further comprise a porcine potato extract.

The deodorant composition may further comprise 1 to 10% by weight of a potato extract based on the total weight of the deodorant composition.

According to the present invention, the polyphenol content of the fermented mushroom extract contains a high content as compared with that before fermentation, and thus exhibits an effective effect. The fermented mushroom extract also includes a deodorant composition including a mushroom extract and a porcine potato extract Disposable absorbent products containing such a deodorant composition are excellent in antimicrobial effect, particularly antimicrobial effect against skin topical bacteria, thereby solving the root cause by preventing or improving skin diseases, Removal, especially the removal of the help to remove.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of analyzing the inhibitory effect of nitrogen monoxide (Nitrite Oxide) formation of the deodorant composition constituting the present invention. FIG.
2 is a graph showing the results of measurement of cytotoxicity against mouse-derived macrophages of the deodorant composition constituting the present invention.
FIG. 3 is a graph showing the result of measuring the change in body weight of a female mouse according to a single toxicity test on a mouse of the deodorant composition constituting the present invention. FIG.
FIG. 4 is a graph showing the result of measuring the change in insecticidal activity of a male mouse according to a single toxicity test on a mouse of a deodorant composition constituting the present invention. FIG.
FIG. 5 is a graph showing the result of measurement of organ weight change of a female mouse according to a single toxicity test on a mouse of a deodorant composition constituting the present invention. FIG.
6 is a graph showing the result of measuring the change in organ weight of a male mouse according to a single toxicity test on a mouse of the deodorant composition constituting the present invention.
Fig. 7 is a graph showing the results of experiments on beneficial bacteria as a graph of the utilization of porcine potatoes as a prebiotics (i.e., a food source) in the deodorant composition constituting the present invention.
Fig. 8 is a graph showing the results of experiments on harmful bacteria as a graph of the utility of the porcine potato as a prebiotic (i.e., a food source) in the deodorant composition constituting the present invention.
FIGS. 9 and 10 are graphs showing that the amounts of IL-6 and TNF-.alpha. Of the deodorant composition constituting the present invention are inhibited in a concentration-dependent manner, respectively.

Hereinafter, specific embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Disposable absorbent products comprising a deodorant composition having an antibacterial and anti-inflammatory activity according to the present invention include an absorbent pad which adheres to a human body and absorbs secretions, wherein the absorbent pad is a deodorant composition .

The absorbent pad closely adheres to the human body, absorbs the secretion secreted from the human body, stores it in the absorbent pad so as not to be discharged to the outside of the disposable absorbent article, and thus has no ability to control defecation / urination such as infants or the elderly It is applied to a weak person and makes it possible to carry out a hygienic life by temporarily holding it in a disposable absorbent product in spite of a sudden defecation / urination. The absorbent pad may be a spunlaced nonwoven fabric for a diaper / sanitary napkin.

The disposable absorbent article includes at least one member selected from the group consisting of a superabsorbent resin, a liquid-permeable upper sheet, a liquid-impermeable lower sheet, a leakage preventing band, and a padding band for tightly fixing and fixing the absorbent pad to a human body, As shown in FIG.

The superabsorbent resin is widely used in disposable absorbent products supplied commercially from domestic and overseas disposable absorbent products manufacturers. The superabsorbent polymer (SAP) has a weight of about 500 to 1,000 times its own weight (Super Absorbency Material) and AGM (Absorbent Gel Material) are named differently for each developer. Such a superabsorbent resin has started to be put into practical use as a sanitary article, and nowadays, in addition to sanitary articles such as diapers for children, there are currently used soil repair agents for horticultural use, index materials for civil engineering and construction, sheets for seedling growing, freshness- And it is widely used as a material for fomentation and the like. As a method of producing such a superabsorbent resin, there are known methods such as reversed-phase suspension polymerization or aqueous solution polymerization. The reversed-phase suspension polymerization is disclosed in, for example, Japanese Unexamined Patent Publication No. 56-161408, Unexamined Japanese Patent Application No. 57-158209, and Japanese Unexamined Patent Publication No. 57-198714. Methods of aqueous solution polymerization include a thermal polymerization method in which the hydrogel polymer is polymerized while being broken and cooled in a kneader having several shafts and a method in which a high concentration aqueous solution is irradiated with ultraviolet rays or the like on a belt to perform polymerization and drying simultaneously Methods, etc., are known, but are not intended to be limited thereto. The superabsorbent resin may be, for example, those described in Korean Patent Laid-Open Publication No. 10-2015-0132035.

The liquid-permeable top sheet functions to be absorbed by the absorbent pad located below the liquid-permeable top sheet, which is located on the upper side of the absorbent pad, i.e., on the side close to the skin, and permeates the liquid-liquid secretion secreted from the human body. So that the liquid secretion does not remain on the upper sheet which directly contacts the skin even when the liquid secretion is secreted from the human body, thereby keeping the skin dry at all times and not causing trouble to the skin.

The liquid-impermeable lower sheet is located on the lower side of the absorbent pad, that is, on the side opposite to the upper sheet. Once the liquid secretion is absorbed by the absorbent pad, even if an external force or the like is applied, To prevent them from being released or released, thus helping to lead a sanitary life.

The leakage preventing band functions as a jaw or an obstacle to prevent the secretion from escaping toward both legs about the absorbent pad, thereby preventing the secretion from being discharged to the outside of the absorbent product.

The pug band is provided with the absorbent pad to fix the disposable absorbent product itself according to the present invention to the body, and is made of a self-adhesive band and is integrally fixed to the absorbent article.

Further comprising at least one member selected from the group consisting of such a superabsorbent resin, a liquid-pervious topsheet, a liquid-impermeable bottomsheet, a leak-barrier band and a puff band, and comprising an absorbent pad positioned between the top sheet and the bottom sheet Disposable absorbent articles made therefrom can be understood to be the same and / or similar to those provided commercially by leading domestic and foreign manufacturers, such as disposable diapers for infants and mothers and adult defenders.

Ecklonia cava used in the preparation of the fermented mash extract is a perennial algae belonging to Laminariales and Alariaceae . It lives in a depth of 10m in the south coastal coast including Jeju Island in Korea. The length is 1 to 2m, stem is cylindrical, and base is roots. As the edible algae, various functionalities such as antioxidant, anticancer and antihypertensive properties have been revealed, and thus they are of great value as new functional materials. In particular, it contains more than 14 polyphenol substances, and studies on physiological activity are known to have excellent cell protection effect, antithrombogenic activity, antioxidant activity, cardioprotective effect and antibacterial activity.

The fermented mushroom extract used in the present invention may be an extract obtained by fermenting a natural plant gut, followed by extraction, preferably with a lower alcohol having 1 to 4 carbon atoms, more preferably with ethanol. The fermentation of the menthol may be preferably yeast fermentation.

Preferably, the fermented extracts are prepared by 1) a YPD (Yeast Peptone Dextrose) medium sterilized at a temperature within the range of 100 to 140 ° C, preferably 110 to 130 ° C for 1 to 30 minutes, preferably 10 to 20 minutes Inoculating yeast Saccharomyces cerevisiae and pre-culturing for 1 to 3 days at 100 to 200 rpm, preferably 130 to 170 rpm, 30 to 40 째 C, preferably 35 to 38 째 C; 2) washing the gangue cleanly, drying it in the shade, and pulverizing it into a powder form; 3) The chelating powder of step 2) and the sterilized water are mixed in a weight ratio of 1: 2 to 7 to obtain a fermentation mixture, which is then added to the fermentation mixture in an amount of 3 to 10% Addition of components); 4) fermenting the fermented mixture of step 3) with 4 to 6% (v / v) of the pre-cultured yeast and fermenting the mixture at 35 to 38 ° C and 100 to 200 rpm for 3 to 7 days to obtain a fermentation product ; 5) The supernatant is separated from the fermented product of step 4) and lyophilized to obtain a fermented powder. The fermented powder is suspended in 10 to 50% ethanol, and the fermented powder is suspended in ethanol at 200 to 400 rpm, 6 hours; 6) extracting the extract of step 5), concentrating it under reduced pressure, and lyophilizing it to obtain a fermented ginger extract in powder form, which may be obtained through a process for producing fermented ginger extract.

The deodorant composition may comprise a solvent as a residual amount of 10 to 50% by weight of the fermented roots extract. When the fermented mash extract is contained in an amount of less than 10% by weight, the effect of the deodorization rate and antibacterial activity of methylmercaptan may be insignificant. On the other hand, when the fermented mash extract is contained in an amount exceeding 50% by weight, There may be problems.

The fermented mushroom extract may be obtained by fermenting mushroom and extracting the mushroom fermentation product obtained by alcohol extraction.

The attritive fermented product may be fermented with yeast.

The deodorant composition may further comprise a plum extract.

The plums used for the production of the plum extract ( Prunus mune Sieb . meat Zucc . ) Is a fruit of plum tree, and the plum tree is a small arboreous tree belonging to Rosaceae and is about 4 ~ 5m in height. White flowers or pink flowers bloom, and fruit ripens in May to June. The country of origin is known as the Sichuan province of China and the mountainous province of Hubei province. In Korea, it is cultivated in a garden or orchard for ornamental purposes in the southern part of the country. It is said that it regulates the liver and the fence in the main herbarium, it releases the internal heat, removes toxins in the body, makes the stool visible, and eliminates the smell of the mouth. Plum is known to have various effects such as fatigue recovery, improvement of constitution, promotion of gastric secretion, improvement of appetite, smooth metabolism, and prevention of constipation. It is also known that citric acid is rich in antimicrobial activity, and it is known that salivary glands stimulate salivary secretion due to acidic taste.

The method for producing the plum extract used in the present invention comprises the steps of: 1) separating and drying the seeds of the cleaned-up browning room and finely crushing the pulp; 2) mixing the dried plum and water in the step 1) at a weight ratio of 1: 2 to 7, and extracting the mixture at 50 to 100 ° C and 200 to 400 rpm for 2 to 6 hours; 3) extracting the extract of step 2), and concentrating the extract to a concentration of 60 to 70 brix using a vacuum concentrator.

The deodorant composition may further comprise 1 to 20% by weight of the plum extract based on the total weight of the deodorant composition. When the amount of the mussel extract is less than 1% by weight, the effect of the deodorization rate and the antibacterial ability of ammonia may be insignificant. On the other hand, when the amount of the mussel extract is more than 20% by weight, Can be.

The deodorant composition may further comprise a porcine potato extract.

The pig potatoes ( Helianthus) used in the preparation of the potato extract tuberosus L. ) is a perennial plant of asteraceae and sunflower, native to North America. Pork potatoes consist of 80% water, 20% carbohydrate, 1-2% protein, minerals and vitamins. Unlike ordinary plants that store carbohydrates in the form of starch, they are known to have the property of storing them as fructose polymeric materials called inulin. Inulin is a water soluble dietary fiber, which is not degraded by animal gastric juices and digestive enzymes. More than 80% of the inulin reaches the large intestine and is used as a growth substrate for intestinal microorganisms and plays a role of prebiotics. Prebiotics produce micro-organisms such as antibiotics, vitamins and growth-promoting factors in the large intestine to improve intestinal function and improve the bioavailability of minerals. It is also known to help smooth bowel movements such as diarrhea and constipation prevention.

The method for preparing the potato extract of the present invention comprises the following steps: 1) slicing a thoroughly washed porcine potato, drying it, and finely crushing it; 2) mixing the dried plum and water in the step 1) at a weight ratio of 1: 2 to 7, and extracting the mixture at 50 to 100 ° C and 200 to 400 rpm for 2 to 6 hours; 3) extracting the extract of step 2), concentrating the extract with a vacuum concentrator, and lyophilizing the extract.

The deodorant composition may further comprise 1 to 10% by weight of a potato extract based on the total weight of the deodorant composition. If the pig potato extract is contained in an amount of less than 1% by weight, there may be a problem that it does not help to play a role of prebiotics. Conversely, if it exceeds 10% by weight, it may help to improve constipation, Rather, there may be a problem causing diarrhea.

The deodorant composition according to the present invention may preferably comprise a solvent as 10 to 50% by weight of the fermented extract, 1 to 20% by weight of a plum extract, 1 to 10% by weight of a potato potato, and the balance.

The solvent may be a glycerol aqueous solution having a concentration of 30 to 70%.

Glycerol is one of the aliphatic trihydric alcohols with the molecular formula of C ₃ H ₅ (OH) ₃ , colorless and odorless, sweet and sticky. It can be used as a drying agent, a solvent, a sweetener, or for preserving food. Glycerin, lysine, propene-1,2,3-triol, 1,2,3-propenetriol, trihydroxypropane, glycerol, glycyl alcohol. It is colorless and transparent, sweet and sticky liquid, strong in hygroscopicity. When strongly pure glycerol is strongly cooled, crystals of melting point 17.8 ° C are obtained, which is prone to undercooling. It is mixed with water and alcohol at an arbitrary ratio, but does not dissolve in hydrocarbons. In 1779, Sweden's KW Scheele was found in the hydrolysis product of olive oil, and in 1836 the composition was determined by TJ Pelouze in France. It is an ester with a fatty acid, and it is widely distributed in plants and animals in the form of oil (fat) or lipid (lipid). However, in recent years, a method of synthesizing propylene produced by cracking, which is a decomposition process of petroleum, through allyl chloride, a method of obtaining as a product of glycerol fermentation (modification of alcohol fermentation) of yeast Are known. It is converted from liver to glucose in the human body and used as energy for cell metabolism, and has a calorie of 4.32 kcal / g. It is used to prevent drying of food by using hygroscopicity.

The deodorant composition according to the present invention can be routinely used in food such as toothpaste, deodorant spray, etc., or chewing gum, candy, confection, drink and the like because it is made of natural materials and has high safety.

The addition amount of 0.5 to 1% by weight to the food or the composition is most appropriate when taking into account the palatability of the food, and 1% by weight or more may be added for the good deodorizing effect.

Therefore, all of the plants used as raw materials of the present invention have been used for a long time as natural substances, and there is no problem about the safety of the deodorant composition containing the extract of these plants as an effective ingredient and the food and drink and compositions containing the same.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be construed as limiting the scope of the invention.

Production Example 1: Preparation of fermented mushroom extract

Sterilization was fermented and extracted for use in the deodorant composition according to the present invention. S. cerevisiae was inoculated into YPD (Yeast Peptone Dextrose) medium and pre-cultured at 37 ° C and 150 rpm for 2 days to ferment the mite. The ratio of the fungus powder to the sterilized water was 1: 5 by weight, and 5% of the sugar was added to the mixture. Yeast cells cultured in the mixture were inoculated with 5% (v / v) and then fermented at 150 rpm and 37 ° C for 5 days. After the fermentation, the culture supernatant was taken by centrifugation at 8,000 rpm for 10 minutes and lyophilized. The lyophilized powder was suspended in 30% ethanol and extracted at 50 ° C and 300 rpm for 4 hours. The extract was filtered with a paper filter, concentrated and lyophilized to obtain a fermented extract. The gentian extract was dried and pulverized, suspended in purified water, and then extracted at 50 ° C and 300 rpm for 4 hours. The extract was filtered with a paper filter, concentrated, and lyophilized to obtain a gentle extract.

Table 1 below shows the results of measuring the total polyphenol content between the mushroom extract and the fermented mushroom extract.

Total polyphenol content (%) Moth extract 30.18% Fermented mint extract 42.53%

Production Example 2: Preparation of plum extract

Water was used as the extraction solvent for use in the deodorant composition according to the present invention. The dried and ground plum was mixed with water at a weight ratio of 1: 5 and extracted at 80 DEG C and 300 rpm for 4 hours. The plum extract was filtered with a paper filter and concentrated to 65 Bricks with a vacuum concentrator to obtain a plum extract.

Production Example 3: Preparation of Pork Potato Extract

Water was used as the extraction solvent for use in the deodorant composition according to the present invention. The dried and ground pork potatoes were mixed in water at a weight ratio of 1: 5 and extracted at 80 DEG C and 300 rpm for 4 hours. The pork potato extract was filtered with a paper filter, concentrated to 30 bricks with a vacuum concentrator, and lyophilized to obtain a potato extract.

Example 1

300 g of the fermented roots extract of Preparation Example 1 was mixed with 700 g of 50% glycerol aqueous solution to obtain a deodorant composition constituting the present invention, which was applied to an absorbent pad and then dried to obtain the antibacterial and anti- A deodorant composition was prepared.

Example 2

300 g of the fermented extract of Preparation Example 1 and 100 g of the mussel extract of Preparation Example 2 were mixed with 600 g of 50% glycerol aqueous solution to obtain a deodorant composition constituting the present invention, To prepare a deodorant composition having antibacterial and anti-inflammatory activity according to the present invention.

Example 3

300 g of the fermented flesh extract of Preparation Example 1 and 50 g of the potato extract of Preparation Example 3 were mixed with 650 g of a 50% glycerol aqueous solution to obtain a deodorant composition constituting the present invention, which was applied to an absorbent pad, A deodorant composition having antibacterial and anti-inflammatory activity according to the present invention was prepared.

Example 4

The deodorant composition constituting the present invention was obtained by mixing 300 g of the fermented extract obtained in Preparation Examples 1 to 3, 100 g of the plum extract, 50 g of the potato extract of pigs and 550 g of the 50% glycerol aqueous solution, And dried to prepare a deodorant composition having antibacterial and anti-inflammatory activity according to the present invention.

Comparative Example 1

For comparison, a composition obtained by mixing 300 g of the menthol extract of Preparation Example 1 and 700 g of 50% glycerol aqueous solution was used as Comparative Example 1.

Comparative Example 2

For comparison, a composition prepared by mixing 300 g of the menthol extract of Preparation Example 1 and 100 g of the plum extract of Preparation Example 2 and 600 g of 50% glycerol aqueous solution was used as Comparative Example 2.

Comparative Example 3

For comparison, a composition obtained by mixing 300 g of the menthol extract of Preparation Example 1 and 50 g of the potato extract of Swine of Preparation Example 3 and 650 g of 50% glycerol aqueous solution was used as Comparative Example 3.

Comparative Example 4

For comparison, a composition prepared by mixing 300 g of the menthol extract of Preparation Example 1, 100 g of the plum extract of Preparation Example 2, and 50 g of a 50% glycerol aqueous solution of 50 g of the potato extract of Preparation Example 3 was used as Comparative Example 4.

Experimental Example 1: Deodorizing effect - Gas detecting tube test method

Deodorizing effect was measured using a gas detection tube (Gastec GV-110S, 71H, Korea) to examine the deodorizing effect of the deodorant composition of the present invention on methyl mercaptan and ammonia.

The vial was mixed with the deodorant composition of the present invention and methyl mercaptan (Wako Pure Chemicals Co., Japan) so that the final concentration was 100 ppm, and the mixture was left at 37 캜 for 10 minutes. After standing, the concentration of methyl mercaptan was measured using a gas detection tube. As a control, distilled water without deodorant composition was used. Also, the method of ammonia (Daejung, Korea) was also measured by the same method as the above-mentioned method.

The odor removing activity of the ginger extract was calculated according to the following equation.

Odor Removal Activity (%) = [(C-S)] * 100

Where C is the measured concentration of the control and S is the measured concentration of the experimental group to which the deodorant composition of the present invention is added.

As a result, it was confirmed that the deodorant composition of the present invention removed 50% of the smell of methyl mercaptan and 75% of the odor of ammonia, and the results are shown in Table 2 below.

division Methyl mercaptan Methyl mercaptan
Odor Removal Activity (%) ammonia ammonia
Odor Removal Activity (%) Control (ppm) 100 0 100 0 Comparative Example 1 (ppm) 90 10 100 0 Example 1 (ppm) 50 50 80 20 Comparative Example 2 (ppm) 90 10 50 50 Example 2 (ppm) 50 50 25 75 Comparative Example 3 (ppm) 90 10 100 0 Example 3 (ppm) 55 45 80 20 Comparative Example 4 (ppm) 90 10 50 50 Example 4 (ppm) 50 50 25 75

Experimental Example 2: Mortality suppression effect

In order to examine the suppression effect, 15 dogs were allowed to consume water for one week in a dog (Maltese, 3 months, male, Dog & Cat Dog Shop, Korea), and the deodorant composition of the present invention ) Was diluted to 1% by weight and allowed to be autonomously ingested. The breeding conditions were set after the temperature was set at 25 ° C and the solid feed (Royal Canine Starter, France) was divided into two portions of 5% of the average weight per day. The ammonia content was measured by the indo-phenol method to measure the ammonia concentration before and after ingestion of the deodorant composition. The results are shown in Table 3 below.

division Before consumption (ppm) After ingestion (ppm) Object 1 403.23 224.12 Object 2 447.72 369.09 Object 3 388.61 253.76 Object 4 510.28 282.33 Object 5 355.66 278.79 Object 6 451.44 314.24 Object 7 461.27 343.53 Object 8 399.54 316.07 Object 9 420.31 184.77 Object 10 388.70 264.92 Object 11 285.04 240.88 Object 12 306.62 190.22 Entity 13 506.63 252.51 Object 14 490.46 370.37 Object 15 412.66 398.87

As shown in Table 3, 15 dogs showed decreased ammonia concentration before and after ingestion. Although there is a difference in each individual, the average tendency is about 30% reduction.

Experimental Example 3: Measurement of Lipopolysaccharide (LPS) -induced nitrogen monoxide (NO) production

The concentration of nitrogen monoxide in the culture liquid was measured using a Griess reaction. First, 96-well culture plates (96 wells) were coated with 1 * 10 ⁶ cells / well of RAW 264.7 cells / well from mouse macrophages using Dulbecco's Modified Eagle Medium (DMEM) containing FBS and antibiotics And the cells were cultured in a 5% CO ₂ (CO ₂ ) incubator at 37 ° C for 24 hours. After 24 hours, the culture medium used for the previous culture was removed, and 1 hour after the disinfecting composition of the present invention (Example 4) was dispensed into a fresh DMEM medium containing no FBS and antibiotics, LPS was treated and cultured for 24 hours. The nitrogen monoxide produced during the culture was measured by the total concentration of nitric oxide present in the cell culture medium using a grease reagent. 50 μl of the cell culture supernatant and 50 μl of the grease reagent were mixed and reacted in a 96-well culture plate for 10 minutes And then absorbance was measured at 540 nm using an ELISA reader. At this time, the control group was made with PBS (Phosphate Buffered Saline). The nitrogen monoxide scavenging activity (%) was expressed by the formula (1-absorbance of the reaction group to which the sample was added / absorbance of the control group to which no sample was added). And IC ₅₀ (ppm), which is a concentration for eliminating 50% of nitrogen monoxide.

As shown in FIG. 1, when the deodorant composition of the present invention and the control group were compared, the production of nitrogen monoxide was suppressed. The IC ₅₀ value for eliminating 50% of the nitrogen monoxide was 174.46 ppm. It can be seen that the deodorant composition of the present invention has an excellent effect in suppressing nitrogen monoxide.

Experimental Example 4: COX-2 enzyme inhibition

10 [mu] l of the deodorant composition of the present invention (Example 4) was added to each of 10 [mu] l of the COX-2 enzyme 10, helium (porphyrin iron complex salt) Lt; / RTI > 10 mu l of arachidonic acid was added thereto, followed by reaction at 37 DEG C for 2 minutes, and then 10 mu l of 1 M hydrochloric acid was added to stop the reaction. SnCl ₂ The reaction solution was diluted 2000 times and 4000 times with EIA buffer, and 50 쨉 l was added to a 96-well plate coated with a prostaglandin antibody. After that, 50 쨉 l of a tracer (PGs screening AChE tracer) and 50 항 of antiserum (antiserum) were added, followed by reaction at room temperature for 18 hours. After the wells were washed 5 times with buffer, 200 E of Ellman's reagent was added and developed for 60 minutes, and the absorbance was measured at 410 nm. As a control group, ibuprofen, which is known to inhibit the COX-2 enzyme, was used. The degree of inhibition of COX activity was expressed by the value of IC ₅₀ (ppm), which is a concentration inhibiting 50% according to the manufacturer's method.

Experimental results are shown in Table 4 below to show that the deodorant composition of the present invention inhibits COX-2 enzyme. IC ₅₀ values of the control and the deodorant composition, which inhibit the COX-2 enzyme by 50%, were 16.3 ppm and 101.39 ppm, respectively. Although the IC ₅₀ value is higher than that of ibuprofen, which is a synthetic drug, it is confirmed that the deodorant composition composed of a natural substance component is superior in COX-2 inhibitory effect.

IC ₅₀ (ppm) COX-2 Control group (ibuprofen) 16.3 Example 1 101.39

Experimental Example 5: DPPH radical scavenging ability

The deodorant composition of the present invention (Example 4) was dissolved by concentration and then mixed in the same amount as DPPH (0.2 mM ethanol solution) (Sigma, USA). After 30 minutes of reaction at room temperature, the absorbance was measured at a wavelength of 520 nm. Vitamin C was used as a control and the relative antioxidative effects were compared. The DPPH radical scavenging activity (%) was calculated from the equation (1 - absorbance of sample added / absorbance of sample not added). The SC ₅₀ values are expressed in ppm in terms of the minimum concentration required to eliminate 50% of the generated radicals, and the results are shown in Table 5 below.

DPPH radical scavenging ability SC ₅₀ (ppm) Control group (vitamin C) 4.52 Deodorant composition 64.01

As shown in Table 5, it was confirmed that the SC ₅₀ values of the control group, vitamin C and the deodorant composition, were 4.52 ppm and 64.01 ppm, respectively. The DPPH radical scavenging ability of the deodorant composition of the present invention was about 1 / 14-fold antioxidant effect.

Experimental Example 6: Cytotoxicity measurement

To determine the toxicity of the deodorant composition of the present invention (Example 4) to the mouse-derived macrophage cell line Raw 264.7 cells, MTT analysis was performed.

Toxicity to macrophages was determined by measuring MTT [(3- (4,5-dimethylthiazol-2yl) -2,5-diphenyl tetrazolium bromide] (Sigma, MO, USA). Raw 264.7 cells were seeded at 1 ⁴ cells / ml per well of 96 wells and then cultured at 37 ° C and 5% CO ₂ for 24 hours. After 24 hours, the medium used for the previous culture was removed, and the samples were treated with each concentration (쨉 g / ml) and cultured at 37 ° C and 5% CO ₂ for 24 hours. After 24 hours, the medium was removed, 0.2% MTT solution was added per well, and the mixture was reacted at 37 ° C and 5% CO ₂ for 3 hours. After the reaction, all of the supernatant was removed, 200 쨉 l of DMSO was added, and all of the formazan produced at room temperature for 30 minutes was dissolved, and the change of absorbance at 570 nm was measured using an ELISA reader. Cell viability (%) was expressed using the following equation. Phosphate buffered saline was used as a control.

Cell survival rate (%) = (absorbance of sample treated group / absorbance of control group)

As shown in FIG. 2, it was confirmed that the cytotoxicity of the deodorant composition of the present invention was not toxic even at a high concentration of 1000 ppm.

Experimental Example 7: Single toxicity test

Seven-week-old Sprague Dawley (SD) rats (Samutago, Korea) were used as experimental animals. Control group and experimental group were divided into 5 male and 5 female rats. These animals were not observed for general symptoms during acquisition and during the adaptation period. The conditions for breeding were set at 22 ℃, 45% relative humidity, 12 hours of illumination (6:00 am to 6:00 pm), and water was added to the water using a laboratory animal feed (Samtago, Korea) (Tap water). The rats were fasted 12 hours before the administration of the sample and were orally administered at two concentrations of 2000 mg / kg and 1000 mg / kg, which are usually used as a single oral dose of the health functional food, and then observed for 14 days. Clinical symptom observations were conducted according to the OECD Test Guideline 420 (TG 420) and the Korea Food and Drug Administration's Toxicological Testing Standards. That is, all the animals were observed every hour for 6 hours after the administration on the day of administration, and the change of the general state of the animal, the expression of the toxic symptoms and the deaths were observed once a day from the next day to the 14th day. The body weight change on the day of administration and on days 1, 3, 7 and 14 of the test substance (deodorant composition according to the present invention (Example 4)) was measured. After completion of the test, the animals were anesthetized and sacrificed, and then the appearance and internal organs were visually observed.

As shown in FIGS. 3 to 6, no deaths occurred in males and females according to the administration. As a result of observing the mice on the same day and observing them once per day for 14 days from the next day, There was no change. Also, as can be seen from Figs. 3 to 6, there was no significant change in the weight change after the administration of the sample and the change in the organ weight after the autopsy compared with the control group. Therefore, it was found that the deodorant composition of the present invention is free of animal monotoxicity.

Experimental Example 8: Use of porcine potato as a prebiotic (i.e., a food source)

The inventive deodorant composition (Example 4) was found to be the most useful bacteria in the human intestine ( Bifidobacterium bifidum ) and harmful bacteria ( Clostridium bifidum ) The growth curves were measured to investigate the effect of growth on the growth of C. perfringens . 10% of the deodorant composition was added to the Reinforced clostridial medium, and 10% of the deodorant composition was added to the Lactobacillus MRS broth for the aerobic bacteria for 72 hours . The degree of propagation of the bacteria was measured at an absorbance of 660 nm at intervals of 12 hours using a spectrophotometer, and the results are shown in FIGS. 7 and 8.

As a result of the experiment, it was found that the growth rate of the bifidum lactic acid bacterium ( B. bifidum ) was increased when 10% of the deodorant composition (Example 4) was added and the growth curve of C. perfrigens was increased. .

Experimental Example 9: Effect of inhibiting the growth of food-borne diseases and other skin diseases-inducing microorganisms

The deodorant composition of Example 4, yet can cause food poisoning microorganisms at the same time that the effect of suppressing the strain using two kinds of Streptococcus aureus (S. aureus), Ricky aeseo control E. coli (E. coli) skin flora Respectively.

The above examination was carried out using a test using a liquid medium. Streptococcus aureus was inoculated into MHB (Mueller Hinton Broth) medium and Escherichia coli was inoculated into LBB (Luria-Bertani broth) medium and cultured at 37 ° C and 150 rpm at 24 After incubation for a time, viable cell counts were determined by the flat plate method. That is, each of the above strains was inoculated into a liquid medium so as to have a concentration of 1 × 10 ⁷ CFU / ml, and the deodorant composition of Example 4 was added thereto at various concentrations, followed by incubation at 37 ° C. and 150 rpm for 24 hours. ) Were measured.

As a result, the MIA values of Staphylococcus aureus and E. coli were 5000 ppm and 20000 ppm, respectively, and thus it was confirmed that they have excellent antibacterial activity.

Examples 5 to 7

The deodorant composition of Example 2 was dissolved in concentration (Example 5: 1%, Example 6: 5%, Example 7: 10%) and 2 ml of the obtained solution was applied to a spunlaced diaper / sanitary napkin 10 * 10 cm, 50 g / m 2) and dried at 60 ° C for 1 hour.

Comparative Example 5

The procedure of Example 5 was repeated except that distilled water was used instead of the deodorant composition as a control.

EXPERIMENTAL EXAMPLE 10: Diaper Deodorizing Effect - Ammonia

The nonwoven fabrics of Examples 5 to 7 and the nonwoven fabric of Comparative Example 5 were placed in a sealed container (3 L), respectively, and then an initial concentration of 100 ppm was obtained by adding 1% ammonia solution. The residual concentration of ammonia gas produced after 1 hour Was measured using a gas detection tube (Gastec GV-100S, Japan).

The deodorizing activity was calculated according to the following equation.

Deodorizing activity (%) = [(C-S)] / C x 100

Wherein C is the measured concentration of Comparative Example 5 and S is the measured concentration of Examples 5 to 7. [

As a result, as shown in Table 6 below, in the case of Comparative Example 5, there was no change in the initial concentration of ammonia, but when the deodorant composition of the present invention was applied, the decrease in the ammonia concentration was measured, , It was found that the odor of ammonia was removed by 100% after one hour. The results are shown in Table 6 below.

ammonia Comparative Example 5 (ppm) Example (ppm) Deodorizing activity (%) Example 5 100 60 40 Example 6 100 12.5 87.5 Example 7 100 0 100

Experimental Example 11: Physiological deodorizing effect - Trimethylamine

Trimethylamine (TMA) is a substance that is produced during the metabolism of our body. Because of the regulation of sex hormones, the female smells of trimethylamine before and after menstruation.

Examples 5 to 7 and Comparative Example 5 were placed in a sealed container (3 L), and then 1% trimethylamine solution was added thereto to make the initial concentration 70 ppm. After 1 hour, the residual concentration of the trimethylamine gas was measured by gas Tube (Gastec GV-100S, Japan).

The deodorizing activity of the deodorant composition was calculated in the same manner as in Experimental Example 10.

As a result, as shown in the following Table 7, in the case of Comparative Example 5, there was no change in the concentration of trimethylamine initially introduced, but when the deodorant composition of the present invention was applied, the decrease in the trimethylamine concentration was measured, It was found that the smell of trimethylamine was removed by 95% after one hour. The results are shown in Table 7 below.

Trimethylamine Comparative Example 5 (ppm) Example (ppm) Deodorizing activity (%) Example 5 70 56 20 Example 6 70 10.5 85 Example 7 70 3.5 95

EXPERIMENTAL EXAMPLE 12: Diaper and Sick House Syndrome Induced Deodorizing Effect - Acetaldehyde

Household adhesives and other volatile organic compounds, such as acetaldehyde and formaldehyde, cause sick house syndrome causing eye, nose and skin irritation. The deodorant effect of the deodorant composition of Example 2 on acetaldehyde was measured as follows. The deodorant composition of Example 2 was dissolved in concentration (10%, 20%, 40%) and 2 ml of the deodorant composition was added to a spunlaced nonwoven fabric (10 * 10 cm, 50 g / m 2) and dried at 60 ° C for 1 hour. A nonwoven fabric (distilled water) was applied to the nonwoven fabric (control) and a nonwoven fabric (10, 20%, 40% ppm, and the residual concentration of the acetaldehyde gas produced after 2 hours was measured using a gas detection tube (Gastec GV-100S, Japan).

The deodorizing activity of the deodorant composition was calculated in the same manner as in Experimental Example 10.

As a result, it was found that when 40% of the deodorant composition of the present invention was applied, 70% of the odor of acetaldehyde was removed after 2 hours. The results are shown in Table 8.

Acetaldehyde Control (ppm) Deodorant composition (ppm) Deodorizing activity (%) 10% deodorant composition 100 80 20 20% deodorant composition 100 60 40 40% deodorant composition 100 30 70

Experimental Example 13: Sick house syndrome induced odor removal effect - Formaldehyde

The removal effect of the deodorant composition of Example 2 on formaldehyde, which is a substance causing the sick house syndrome, was measured by the following method. That is, the deodorant composition was dissolved by concentration (10%, 20%, 40%) and 2 ml was added to a spun lace nonwoven fabric (10 * 10 cm, 50 g / m 2) and dried at 60 ° C for 1 hour. (10%, 20%, 40%) by using distilled water instead of deodorant composition (control) and 10% formaldehyde solution were added to each sealed container (3 ℓ) 80 ppm, and the residual concentration of the formaldehyde gas produced after 2 hours was measured using a gas detection tube (Gastec GV-100S, Japan).

The deodorizing activity of the deodorant composition was calculated in the same manner as in Experimental Example 10.

As a result, it was found that when 40% of the deodorant composition of the present invention was applied, the odor of formaldehyde was removed by 50% after 2 hours. The results are shown in Table 9.

Formaldehyde Control (ppm) Deodorant composition (ppm) Deodorizing activity (%) 10% deodorant composition 80 70 12.5 20% deodorant composition 80 60 25 40% deodorant composition 80 40 50

Experimental Example 14: Stability against odor removing effect of deodorant composition - Ammonia

The stability of the deodorant composition of Example 2 against ammonia removal efficacy was measured by the following method. After dissolving the deodorant composition at 10%, 2 ml was added to a spunlaced nonwoven fabric (10 * 10 cm, 50 g / m 2) and dried and stored at 60 ° C for 1 hour, 48 hours and 120 hours, respectively. A 1% ammonia solution was added to the sealed vessel (3 L) to obtain a concentration of 100 ppm, and the following experiment was conducted. A nonwoven fabric (distilled water) and a nonwoven fabric (1 hour, 48 hours, and 120 hours' drying) using a 10% deodorant composition were placed in a sealed container (3 L) 100 ppm, and the residual concentration of ammonia gas produced after 1 hour was measured using a gas detection tube (Gastec GV-100S, Japan).

As a result, when the deodorant composition of the present invention was applied at 10%, the odor of ammonia was removed by 97.5% when stored at 60 ° C for 120 hours, showing deodorizing effect similar to that of the 1-hour dried sample (100%). Therefore, when the deodorant composition of the present invention was stored at 60 ° C. for 120 hours, it was found that the deodorizing effect was stable, and the results are shown in Table 10.

ammonia Control (ppm) Deodorant composition (ppm) Deodorizing activity (%) 1 hour drying 100 0 100 48 hours drying 100 0 100 120 hours drying 100 2.5 97.5

EXPERIMENTAL EXAMPLE 15: Stability of the deodorant composition against odor removing efficacy -Trimethylamine

The stability of the deodorant composition of the present invention against the trimethylamine removal efficacy was measured by the following method. After dissolving the deodorant composition at 10%, 2 ml was added to a spunlaced nonwoven fabric (10 * 10 cm, 50 g / m 2), and dried and stored at 60 ° C for 1 hour, 48 hours and 120 hours. A nonwoven fabric (distilled water) and a nonwoven fabric (1 hour, 48 hours, 120 hours drying) using a 10% deodorant composition were placed in a sealed container (3 L), and a 1% And the residual concentration of trimethylamine gas produced after 1 hour was measured using a gas detection tube (Gastec GV-100S, Japan).

As a result, when the deodorant composition of the present invention was applied at 10%, it was found that the smell of trimethylamine was removed by 90% when stored at 60 ° C for 120 hours. Thus, the same effect as the 1-hour dried sample (95%) was shown. Therefore, it was found that the deodorizing effect was stable when the 10% deodorant composition was stored at 60 ° C for 120 hours, and the results are shown in Table 11.

Trimethylamine Control (ppm) Deodorant composition (ppm) Deodorizing activity (%) 1 hour drying 70 3.5 95 48 hours drying 70 3.5 95 120 hours drying 70 7 90

Experimental Example 16: Measurement of LPS induced IL-6 and TNF-a production

After induction of inflammatory response with LPS, concentrations of IL-6 and TNF-α in cell culture medium were measured as follows. First, RAW 264.7 cells derived from mouse macrophages at 1 x 10 ⁶ cells / ml were seeded in a 24-well culture plate using DMEM medium, and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours. After 24 hours, the deodorant composition of the present invention was divided into different DMEM medium (25, 50, 100, 200 占 퐂 / ml) for 1 hour and then treated with 1 占 퐂 / ml of LPS and cultured for 24 hours. IL-6 and TNF-α in the culture medium were measured using an ELISA kit (R & D Systems, Minneapolis, MN). The control group was PBS (Phosphate Buffered Saline).

As shown in FIG. 9 and FIG. 10, when the deodorant composition of the present invention and the control group were compared, the amounts of IL-6 and TNF-α were inhibited in a concentration-dependent manner, respectively. It was confirmed that the deodorant composition of the present invention has an excellent effect on inhibition of inflammation by inhibiting IL-6 and TNF- ?.

The present invention can be used in industries that manufacture and use health foods and hygiene products that require deodorant function in particular.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

(Without reference numerals)

Claims (9)

A disposable absorbent article comprising a deodorant composition comprising an antibacterial, anti-inflammatory activity deodorant composition comprising an absorbent pad in close contact with the body to absorb secretions, wherein the absorbent pad comprises a fermented flesh extract as an active ingredient product. The method according to claim 1,
Wherein the disposable absorbent article comprises at least one member selected from the group consisting of a superabsorbent resin, a liquid-permeable upper sheet, a liquid-impermeable lower sheet, a leak-barrier belt, and a puddle band for closely fixing and fixing the absorbent pad to a human body, Further comprising a deodorant composition having antibacterial, anti-inflammatory activity. The method according to claim 1,
Characterized in that the deodorant composition comprises 10 to 50% by weight of the fermented phytotoxic extract and the remainder comprises a solvent, and a deodorant composition having antibacterial, anti-inflammatory activity. The method according to claim 1,
The disposable absorbent article according to any one of claims 1 to 3, wherein the fermented mushroom extract has alcohol-extracted mushroom fermented product obtained by fermenting menthol, and a deodorant composition having antibacterial and anti-inflammatory activity. 5. The method of claim 4,
Wherein the fermented product is fermented with yeast, wherein the fermented product is fermented with yeast. The method according to claim 1,
A disposable absorbent article comprising a deodorant composition having antibacterial, anti-inflammatory activity, characterized in that the deodorant composition further comprises a plum extract. The method according to claim 6,
Characterized in that the deodorant composition further comprises an amount of 1 to 20% by weight of the plum extract, based on the total weight of the deodorant composition, of a deodorant composition having antibacterial, anti-inflammatory activity. The method according to claim 1,
Wherein said deodorant composition further comprises a porcine potato extract. ≪ RTI ID = 0.0 > 11. < / RTI > A disposable absorbent article comprising a deodorant composition having antimicrobial, anti-inflammatory activity. 9. The method of claim 8,
Characterized in that the deodorant composition further comprises 1 to 10% by weight of a potato extract of pigs based on the total weight of the deodorant composition.

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