WO2017181835A1 - Procédé de mise en place d'un modèle animal d'hypertension oculaire chronique - Google Patents

Procédé de mise en place d'un modèle animal d'hypertension oculaire chronique Download PDF

Info

Publication number
WO2017181835A1
WO2017181835A1 PCT/CN2017/078965 CN2017078965W WO2017181835A1 WO 2017181835 A1 WO2017181835 A1 WO 2017181835A1 CN 2017078965 W CN2017078965 W CN 2017078965W WO 2017181835 A1 WO2017181835 A1 WO 2017181835A1
Authority
WO
WIPO (PCT)
Prior art keywords
animal model
schlemm
ocular hypertension
establishing
catheter
Prior art date
Application number
PCT/CN2017/078965
Other languages
English (en)
Chinese (zh)
Inventor
梁远波
胡城
廖娜
徐晶
Original Assignee
温州眼视光发展有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 温州眼视光发展有限公司 filed Critical 温州眼视光发展有限公司
Priority to JP2018532502A priority Critical patent/JP6505325B2/ja
Publication of WO2017181835A1 publication Critical patent/WO2017181835A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D1/00—Surgical instruments for veterinary use

Definitions

  • the present invention relates to the field of medical animal models, and in particular to a method for establishing a chronic high intraocular pressure animal model.
  • Glaucoma is an irreversible optic nerve degenerative disease, the second blind eye disease in the world. The complex pathogenesis is still not clear. Although high intraocular pressure is not the only factor for glaucomatous visual impairment, high intraocular pressure is one of the most dangerous factors in the development and development of glaucomatous optic nerve damage. In the field of glaucoma research, animal models have become an important research tool. The glaucoma animal model plays an important role in better understanding the mechanism of injury and treatment of glaucomatous optic neuropathy. High intraocular pressure is the most important risk factor for glaucoma optic nerve damage.
  • the animals currently used to make glaucoma models are lower mammals, mainly rabbits, mice, and monkeys. Although the mouse is easy to be transformed and propagated by transgenic technology, and the structure of the mouse eye is similar to the human eye in many aspects, such as trabeculae, Schlemm tube, ciliary body, retinal blood vessels, etc., the mouse eye is relatively small, which is not conducive to surgical operation. .
  • the laser-induced high intraocular pressure model of monkeys is currently recognized as the most ideal animal model of high intraocular pressure. However, due to its high cost and complicated operation, it is not widely used in China.
  • Rabbits are the most commonly used experimental animals for making glaucoma models because they are inexpensive, docile, easy to obtain and operate.
  • anterior chamber angle (and trabecular) type such as anterior chamber injection of hyaluronic acid, methyl cellulose, blood cells, compound carbomer solution, posterior chamber Injection, chymotrypsin, laser photocoagulation trabecular meshwork, topical cortisol and other methods; effect on the scleral aqueous reflux vein type such as the common scleral surface intravenous hypertonic saline method (Morre-Morrison model), burning sclera surface The aqueous humor drainage method (Shareef-Sharma model) and improved methods; in addition, there are some special species Hairy mice and transgenic techniques construct glaucoma models, such as DBA/2J and AKXD-28/Ty pure mice, natural glaucoma models constructed using gene targeting techniques.
  • RGCs retinal ganglion cells
  • the clinical model and animal modeling methods provide a basis for further study of the mechanism of chronic high intraocular pressure glaucoma and provide new thinking for its treatment.
  • the present invention provides a method for establishing a chronic high intraocular pressure animal model.
  • the technical solution adopted by the present invention is: A method for establishing a chronic high intraocular pressure animal model, comprising the following steps: (1) binding the model animal to the operating table, disinfecting the eye towel, placing the tissue Routine, flush the conjunctival sac, and perform local anesthesia under the conjunctiva of the operation eye, and fix the suture of the upper rectus muscle; (2) According to the direction of the dial of the clock, cut the coronal conjunctiva from 11 o'clock to 1 o'clock, to 12 o'clock A shallow scleral flap with a size of 4*4 mm and a 1/3 scleral thickness with a limbus as the base, and a deep sclera of 3*3 mm in thickness and 2/3 scleral thickness below the superficial scleral flap.
  • the conjunctival sac is washed with a diluted iodophor solution.
  • 0.4 ml of 2% lidocaine was used for subconjunctival local anesthesia.
  • a polymer hyaluronic acid is injected into both ends of the Schlemm tube by using a 30G needle.
  • the fiber conduit in the step (3) comprises a cylindrical solid conduit, and the conduit has a spherical structure at one end.
  • the fiber conduit is 10 cm long and has a diameter of 200 ⁇ m, and the spherical diameter of one end of the conduit is 250 ⁇ m.
  • the fiber conduit is made of a polypropylene material.
  • the beneficial effects of the present invention are:
  • the present invention provides a method for establishing a chronic high intraocular pressure animal model, which is a chronic glaucoma animal model in which the intraocular pressure is blocked by surgically placing a material, thereby causing an increase in intraocular pressure.
  • the chronic ocular hypertension animal model produced by this method can be adjusted according to the range of occlusion Schl emm. It is also possible to select fiber guides of different sizes according to the Schlemm pipe diameter of different animal types, and complete the Schlemm tube blockage, thereby producing a chronic high intraocular pressure model of various animal types. In the study of chronic high intraocular pressure glaucoma mechanism, it is better to construct a model animal and modeling method that is compatible with the clinical, and provide a basis for the mechanism of optic nerve injury in chronic glaucoma.
  • FIG. 1 is a schematic view showing the structure of a fiber conduit of the present invention.
  • the present invention will be further described with reference to FIG. 1. Operation steps: The operation requires a long 10 cm diameter 200 ⁇ solid fiber catheter made of polypropylene material, and the tip end of the catheter has a diameter of 250 ⁇ , which is partially expanded to prevent intubation. Damage to the Schlemm wall tissue.
  • the model animal limbs are tied to the operating table, the eye is routinely disinfected and spread, placed in a device, diluted with iodophor solution to wash the conjunctival sac, and 0.4 ml of 2% lidocaine is applied to the subconjunctival local anesthesia. After that, the upper rectus traction suture is fixed, and according to the direction of the dial of the clock, the conjunctiva is cut at the edge of 11 o'clock to 1 o'clock, and the size of the corneal margin is about 4*4 mm with the center of 12 o'clock as the center.
  • a deep scleral flap with a size of about 3*3 mm and a thickness of about 2/3 scleral thickness is made under the superficial scleral flap. Peel off the cornea, find the Schlemm tube and cut the outer wall of the Schlemm tube, and inject the polymer hyaluronic acid (Healon GV) into the ends of the Schlem m tube with a 30G needle to make the catheter easily pass through the mouth.
  • Healon GV polymer hyaluronic acid
  • the anterior chamber puncture was performed in the direction of the flat iris.
  • the posterior margin of the puncture was lightly pressed, and the right amount of aqueous humor was released to reduce the intraocular pressure.
  • the microcatheter was inserted into the Schlemm tube.
  • Broken end according to the required target intraocular pressure, respectively, follow the Schlemm tube 360 degrees or 270 degrees or 180 degrees or 90 degrees, cut the catheter, place it in Schlemm to block the Schlemm tube, prevent the outflow of water, resulting in A model of elevated intraocular pressure.
  • 4-6 needles of the scleral flap were tightly sutured with a 10-0 polypropylene thread.
  • the anterior chamber puncture was infused with a balanced salt solution, and the water-tight suture was observed as a non-leakage, and the conjunctival flap was sutured with a 10-0 polypropylene thread.
  • Anti-inflammatory drugs were given after surgery to monitor intraocular pressure and anterior segment response.
  • the present invention is a glaucoma animal model in which the intraocular water outflow channel is blocked by a surgically placed material, causing an increase in intraocular pressure to damage the optic nerve.
  • the chronic high intraocular pressure animal model produced by this method can be based on the occlusion of Schlemm. The range is different for regulation. It is also possible to select fiber guides of different sizes according to the Schlemm pipe diameter of different animal types, and complete the Schlemm tube blockage, thereby producing a slow high intraocular pressure model of various animal types. .
  • the animal models of rabbit eyes and monkey eyes use a fiber guide that can be used with a diameter of 10 cm and a diameter of 200 ⁇ m, and the tip of the catheter has a spherical diameter of 250 ⁇ m. Other animals can choose a catheter size that matches the Schlemm diameter.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Surgery (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Materials For Medical Uses (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La présente invention concerne un procédé de mise en place d'un modèle animal d'hypertension oculaire chronique. Le procédé comprend : la création d'un modèle de glaucome chez l'animal présentant une pression oculaire élevée et des nerfs optiques détériorés en introduisant chirurgicalement un matériau en vue de bloquer un trajet d'évacuation d'une humeur aqueuse, de manière que la pression oculaire élevée soit maintenue et stabilisée, et puisse être obtenue et manipulée plus facilement. Le modèle obtenu présente les avantages tels qu'une élévation stable et de petites fluctuations de la pression oculaire, la pression oculaire élevée peut être maintenue pendant longtemps et la pression oculaire cible peut être contrôlée. Un modèle animal d'hypertension oculaire chronique peut être fabriqué à l'aide du procédé, et sa pression oculaire peut être réglée et contrôlée selon différents degrés de blocage du canal de Schlemm ; le blocage du canal de Schlemm peut être obtenu en sélectionnant des conduits fibreux de différentes caractéristiques et tailles sur la base des différents diamètres des canaux de Schlemm chez différents types d'animaux, produisant ainsi des modèles animaux d'hypertension oculaire chronique destinés à des types multiple d'animaux. Le procédé permet la mise en place d'animaux constituant un modèle cliniquement compatible et des procédés d'élaboration de modèle destinés à la recherche dans le mécanisme de glaucome d'hypertension oculaire chronique, et fournit une fondation au mécanisme de détérioration du nerf optique dans le glaucome chronique.
PCT/CN2017/078965 2016-04-21 2017-03-31 Procédé de mise en place d'un modèle animal d'hypertension oculaire chronique WO2017181835A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2018532502A JP6505325B2 (ja) 2016-04-21 2017-03-31 慢性高眼圧非ヒト動物モデルの作製方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610249471.5A CN105943186A (zh) 2016-04-21 2016-04-21 一种慢性高眼压动物模型的建立方法
CN201610249471.5 2016-04-21

Publications (1)

Publication Number Publication Date
WO2017181835A1 true WO2017181835A1 (fr) 2017-10-26

Family

ID=56917874

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2017/078965 WO2017181835A1 (fr) 2016-04-21 2017-03-31 Procédé de mise en place d'un modèle animal d'hypertension oculaire chronique

Country Status (3)

Country Link
JP (1) JP6505325B2 (fr)
CN (1) CN105943186A (fr)
WO (1) WO2017181835A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109907857A (zh) * 2019-02-28 2019-06-21 中国人民解放军第四军医大学 一种小鼠眼球前方注射聚酯纤维微球的装置
CN111110441A (zh) * 2020-01-08 2020-05-08 孙河 一种实验用大鼠巩膜静脉注射装置及方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105943186A (zh) * 2016-04-21 2016-09-21 温州眼视光发展有限公司 一种慢性高眼压动物模型的建立方法
CN107981969B (zh) * 2017-12-29 2023-07-14 苏州朗目医疗科技有限公司 一种青光眼内引流替代仿生支架
CN109481071B (zh) * 2019-01-16 2021-07-23 沈阳眼产业技术研究院有限公司 一种用于建立慢性高眼压动物模型的物品
CN111053626B (zh) * 2019-12-26 2021-08-20 中国人民解放军总医院 一种造模方法及动物模型及其应用
CN113133431A (zh) * 2021-02-25 2021-07-20 中南大学 慢性高眼压合并长眼轴动物模型的建立方法、模型及应用
CN113273546A (zh) * 2021-02-25 2021-08-20 中南大学 聚桂醇在制备慢性高眼压动物模型中的应用及动物模型

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011046949A2 (fr) * 2009-10-12 2011-04-21 The Regents Of The University Of Colorado, A Body Corporate Implants destinés à réduire la pression intraoculaire
CN102596121A (zh) * 2009-08-20 2012-07-18 格里沙贝眼科研究基金会 治疗青光眼的方法和装置
CN103002842A (zh) * 2010-05-27 2013-03-27 I科学干预公司 用于把周向植入物放置在巩膜静脉窦中的装置
CN105943186A (zh) * 2016-04-21 2016-09-21 温州眼视光发展有限公司 一种慢性高眼压动物模型的建立方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5360399A (en) * 1992-01-10 1994-11-01 Robert Stegmann Method and apparatus for maintaining the normal intraocular pressure
JP2002112664A (ja) * 2000-10-04 2002-04-16 Koken Co Ltd 緑内障モデル動物の作成方法
JP2003149236A (ja) * 2001-08-30 2003-05-21 Santen Pharmaceut Co Ltd 緑内障治療薬のスクリーニング方法
WO2004042001A2 (fr) * 2002-05-17 2004-05-21 Emory University Particules pseudovirales, procede de fabrication et compositions immunogeniques
US8366653B2 (en) * 2007-03-13 2013-02-05 University Of Rochester Intraocular pressure regulating device
US8434489B2 (en) * 2009-10-23 2013-05-07 Conceptus, Inc. Contraceptive devices and methods
NL2004047C2 (en) * 2010-01-04 2011-07-05 D O R C Dutch Ophthalmic Res Ct International B V An ophthalmic surgical device and a method of performing ophthalmic surgery.
CN102824238B (zh) * 2011-06-16 2014-07-09 王宁利 一种施莱姆氏管（Schlemm）扩张支架及其组合体
CN202637223U (zh) * 2012-04-24 2013-01-02 东华大学 一种不对输卵管组织产生损伤的阻塞型节育器
CN203355084U (zh) * 2013-07-10 2013-12-25 高广林 鼻胆引流管

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102596121A (zh) * 2009-08-20 2012-07-18 格里沙贝眼科研究基金会 治疗青光眼的方法和装置
WO2011046949A2 (fr) * 2009-10-12 2011-04-21 The Regents Of The University Of Colorado, A Body Corporate Implants destinés à réduire la pression intraoculaire
CN103002842A (zh) * 2010-05-27 2013-03-27 I科学干预公司 用于把周向植入物放置在巩膜静脉窦中的装置
CN105943186A (zh) * 2016-04-21 2016-09-21 温州眼视光发展有限公司 一种慢性高眼压动物模型的建立方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LI, PINGYU ET AL.: "Glaucoma Model of Rabbit and Mice", OPHTHALMOLOGY IN CHINA, vol. 13, no. 2, 31 December 2004 (2004-12-31), pages 116 - 119 *
LING, ZHIHONG ET AL.: "Establishment of a Rat Chronic Elevated Intraocular Pressure Model by Episcleral Veins Ligation and Subconjunctival 5-Fu Injection", ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA, vol. 14, no. 3, 30 September 2006 (2006-09-30), pages 172 - 175 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109907857A (zh) * 2019-02-28 2019-06-21 中国人民解放军第四军医大学 一种小鼠眼球前方注射聚酯纤维微球的装置
CN109907857B (zh) * 2019-02-28 2023-10-31 中国人民解放军第四军医大学 一种小鼠眼球前方注射聚酯纤维微球的装置
CN111110441A (zh) * 2020-01-08 2020-05-08 孙河 一种实验用大鼠巩膜静脉注射装置及方法

Also Published As

Publication number Publication date
JP6505325B2 (ja) 2019-04-24
CN105943186A (zh) 2016-09-21
JP2018527147A (ja) 2018-09-20

Similar Documents

Publication Publication Date Title
WO2017181835A1 (fr) Procédé de mise en place d'un modèle animal d'hypertension oculaire chronique
CN103932841B (zh) 一种施莱姆氏管(Schlemm)扩张支架及其组合体
US10238536B2 (en) Ocular filtration devices, systems and methods
TWI491389B (zh) 用於青光眼手術之小型機械式組織切割器／吸引器探針
US20110202049A1 (en) Small Gauge Ablation Probe For Glaucoma Surgery
US11707383B2 (en) Inner drainage biomimetic stent for glaucoma and use thereof
EP2521517B1 (fr) Dispositif ophtalmique chirurgical
KR20040036912A (ko) 녹내장을 처리하기 위한 개선된 분로 장치 및 방법
MXPA05008675A (es) Metodos y dispositivos para drenar fluidos y reducir presion intraocular.
WO2016168686A1 (fr) Dispositifs, systèmes et procédés de filtration oculaire
WO2022268143A1 (fr) Dispositif de drainage d'humeur aqueuse implantable dans l'œil de patient atteint de glaucome
CN110037854B (zh) 一种青光眼房水引流装置
CN201631477U (zh) 青光眼微创房水引流植入物
US10076443B2 (en) Penetrating canaloplasty for treating angle-closure glaucoma
RU2548513C1 (ru) Способ микроинвазивной непроникающей глубокой склерэктомии
CN102406526A (zh) 眼前房角支架
Eliassi-Rad et al. Microinvasive glaucoma surgery (MIGS)
Mishra et al. Glaucoma drainage device: A review
Javed Phacoemulsification under Topical Anesthesia Alone Versus Topical Anesthesia with Subconjunctival
O'BRIEN Surgical treatment of primary glaucoma
RU2500367C1 (ru) Способ хирургического лечения рефрактерной глаукомы в терминальной стадии
Figueiredo Gonioscopic Surgery: Trabecular Micro-Bypass Stent Implantation
RU2357712C1 (ru) Способ выполнения гидродиссекции при экстракции катаракты
Chan et al. 17 Nonpenetrating Glaucoma Surgery
Caretti et al. Ab Internal Stent 12

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref document number: 2018532502

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17785320

Country of ref document: EP

Kind code of ref document: A1

122 Ep: pct application non-entry in european phase

Ref document number: 17785320

Country of ref document: EP

Kind code of ref document: A1