WO2017148449A1 - 适用于振动切片的自动切片收集装置及方法 - Google Patents

适用于振动切片的自动切片收集装置及方法 Download PDF

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Publication number
WO2017148449A1
WO2017148449A1 PCT/CN2017/077159 CN2017077159W WO2017148449A1 WO 2017148449 A1 WO2017148449 A1 WO 2017148449A1 CN 2017077159 W CN2017077159 W CN 2017077159W WO 2017148449 A1 WO2017148449 A1 WO 2017148449A1
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WIPO (PCT)
Prior art keywords
slice
pipe
valve
water pump
hole
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PCT/CN2017/077159
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English (en)
French (fr)
Inventor
龚辉
袁菁
江涛
邓磊
骆清铭
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Huazhong University of Science and Technology
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Huazhong University of Science and Technology
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Priority to JP2017549378A priority Critical patent/JP6543350B2/ja
Priority to US15/691,698 priority patent/US10197476B2/en
Publication of WO2017148449A1 publication Critical patent/WO2017148449A1/zh
Anticipated expiration legal-status Critical
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/18—Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G01N2001/061—Blade details
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/18—Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
    • G01N2001/185—Conveyor of containers successively filled
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S83/00—Cutting
    • Y10S83/9155—Microtome

Definitions

  • the invention relates to a preparation device for biological thin slices, in particular to an automatic slice collection device and method suitable for vibration slice.
  • U.S. Patent No. 2,001,030,022 describes a device for automatic slice collection by a conveyor belt, which utilizes a belt to attach a resin-embedded tissue section produced by an ultra-thin slicer, and collects and stores a conveyor belt to which a slice has been attached by means of a roller or the like; Ultra-thin sectioning of resin-embedded samples in air, vibrating sections are carried out in a liquid environment, and the resulting soft tissue sections cannot be attached to a conveyor belt, so the belt-type section collecting device is not suitable for vibration Slice method.
  • 20130019725 describes a device for automatic slice collection by controlling the flow of water, which uses water flow to collect the slices through different water channels into the multi-well plate, but the multiple parallel water paths designed are repeated and complicated, and difficult. Ensure that each generated slice enters the corresponding pipeline accurately, affecting the efficiency of slice collection.
  • the existing slice collection device suitable for the vibrating slicer has a complicated structure, and a large number of modifications and adaptations of the vibration slicer are required. These problems limit the development and application of such methods in practical applications. Therefore, it is necessary to study a simple and efficient automatic slice collection device suitable for vibration slices.
  • the object of the present invention is to overcome the above deficiencies of the prior art and to provide a vibration slice suitable for use.
  • the automatic slice collection device and method can realize automatic collection of sample slices generated during the vibration slice process, and improve slice and operation efficiency.
  • the technical solution adopted for achieving the object of the present invention is an automatic slice collecting device suitable for vibration slicing, the device comprising:
  • the first pipe has a port at one end facing the slice cut by the vibrating slicer, and the other end is connected with a water pump with a positive and negative motor; the first pipe is provided with a first valve, and a filter is arranged between the water pump and the first pipe. Net;
  • the second pipe has one end connected between the first valve of the first hose and the water pump, and the second pipe is provided with a second valve.
  • the other end of the second pipe is provided with a dispensing head, and a porous plate is arranged below the dispensing head.
  • the dispensing head is sleeved with an annular filter, and the diameter of the annular filter is the diameter of the hole in the porous plate.
  • the vibrating slicer comprises a blade holder and a blade mounted on the blade holder, wherein the blade holder is provided with a hollow through hole, one end of the through hole is opposite to the blade, and the other end is opposite The first pipe is connected.
  • the apparatus of the present invention further includes a three-axis robot arm, and the first valve, the second valve, and the dispensing head are respectively disposed on the three-axis robot arm.
  • the water pump communicates with the water tank through the third duct.
  • the apparatus of the present invention further includes a sump in which the perforated plate is mounted; the sump is in communication with the sink through the fourth conduit.
  • the present invention also provides a method for implementing automatic slice collection by the above device, the cover method comprising:
  • Closing the second valve opening the first valve, controlling the forward rotation of the water pump motor, and the slice cut by the vibrating slicer flows into the first pipe with the buffer; the filter screen prevents the slice from flowing out from the first pipe;
  • the annular screen on the dispensing head prevents the slices from escaping from the holes of the multi-empty plate; the buffer overflowing from the holes is collected by the sump and returned to the water tank through the fourth pipe.
  • the structure is simple, easy to adapt to different vibrating slicers.
  • Figure 1 is a block diagram showing a preferred embodiment of an automatic slice collection device suitable for use in vibrating sections of the present invention.
  • FIG. 2 is a schematic view showing a three-dimensional structure of a preferred embodiment of an automatic slice collecting device suitable for vibrating sectioning of the present invention.
  • Fig. 3 is a schematic view showing the structure of a vibrating slicer used in the embodiment.
  • Figure 4 is a flow chart showing the operation of automatic slice collection using the apparatus of the present invention.
  • the embodiment is applicable to an automatic slice collecting device for vibrating sectioning, and its structure is as shown in FIG. 1.
  • the device comprises a water tank 15 in which a buffer 22 is placed, and the biological sample 16 and the vibrating slicer are both located in the buffer 22 to Ensure that the slicing process is carried out in a liquid environment.
  • the structure of the vibrating slicer used in this embodiment is as shown in FIG. 2, and includes a blade holder 19 and a blade 18 provided on the blade holder 19.
  • the nut is screwed into the screw hole (25, 26) to fasten the blade 18, and the blade holder
  • a through hole 20 is provided in the upper end of the through hole 20 opposite the blade edge of the blade 18.
  • the automatic slice collecting device applicable to the vibration slice of the embodiment further includes a three-way sump 3, a pipe 10, a pipe 11, a pipe 12, a pipe 13, a pipe 14, and a perforated plate 6, wherein
  • One end of the pipe 10 is connected to the first passage of the three-way sump 3, and the other end is connected to the upper end 20 of the through hole.
  • the side of the through hole 20 is provided with an expansion end 27, and the expansion end 27 is convenient for the connection with the pipe 10.
  • a first valve 1 is disposed on the pipe 10, the first valve 1 is used to control the opening and closing of the pipe 10, and the first valve 1 can be an electromagnetic pinch valve.
  • One end of the pipe 11 is connected to the second passage of the three-way sump 3, the other end is connected with a sub-head 4, the perforated plate 6 is located below the sub-assembly head 4, and the sub-head 4 is sleeved with an annular filter 5, a ring
  • the diameter of the screen 5 is the same as the diameter of the hole in the perforated plate 6, and when the dispensing head 4 projects into the hole of the perforated plate 6, the annular screen 5 can come into contact with the inner wall around the hole.
  • a second valve 2 is provided on the hose, the second valve 1 is used to control the opening and closing of the hose 10, and the second valve 1 can be an electromagnetic pinch valve.
  • the perforated plate 6 is placed in the sump 7, and the sump 7 is in communication with the water tank 15 through the pipe 14.
  • One end of the hose 13 is connected to the third passage of the three-way hopper 3, and a filter 8 is provided at the joint, and a water pump 9 with a forward and reverse motor is provided at the other end.
  • the water pump 9 is also in communication with the water tank 15 via a hose 13.
  • the automatic slice collecting device applicable to the vibration slice of the embodiment further comprises a three-axis mechanical arm 28, a first valve 1, a second valve 2, and a three-way stor 3 and the dispensing head 4 are disposed on the three-axis robot arm 28 as shown in FIG.
  • each component in the automatic slice collection device of the present embodiment is as follows: the first valve 1 and the second valve 2 are electromagnetic pinch valves; the three-way hopper 3 and the dispensing head 4 are plastic processing parts; the annular filter 5 and The filter screen 8 is processed by a stainless steel filter screen; the multi-empty plate 6 is a 24-hole plate made of polystyrene, the liquid collection tank 7 and the water tank 15 are made of aluminum, and the water pump 9 is a gear pump driven by a DC brushless motor.
  • the volume adjustment range is 50-1000 ml/min; the pipe 10, the pipe 11, the pipe 12, the pipe 13, and the pipe 14 are all silicone pipes, having an inner diameter of 6.4 mm and an outer diameter of 9 mm; and the blade 18 is a zirconia blade of Electron Microscopy Sciences.
  • the blade holder 19 is a stainless steel machined part; the three-axis machine arm 28 is a three-axis machine arm of the type T6L-T5L-T4L manufactured by Yamaha Corporation.
  • FIG. 4 is a working flow chart of the automatic slice collecting device applicable to the vibration slice of the embodiment. The following describes the working process of the device in the following with reference to the components of the device of the embodiment:
  • Step S1 the second valve 2 is closed, the first valve 1 is opened, and the water pump 9 is controlled to rotate at a low speed to form a low-speed stable water flow in the liquid flow direction 23 along the forward rotation of the water pump motor, and the buffer 22 is opened from the lower end of the through hole 20 It enters the through hole, and then flows through the pipe 10, the three-way hopper 3, the screen 8, the pipe 11, the water pump 9, and the pipe 13 from the upper end of the through hole 20, and then flows back into the water tank 15.
  • Step S2 the vibrating slicer drives the blade 18 to start slicing the biological sample 16, and the sample slice 17 generated during the slicing process is opened from the lower end of the through hole 20 under the push of the low-speed stable water flow in the liquid flow direction 23 when the water pump motor rotates forward. Entering the through hole, the water flow rate is lower to avoid the sample slice 17 being torn.
  • Step S3 after the blade 18 completes the slicing of the entire section of the biological sample 16, the water pump 9 increases the rotational speed to form a high-speed stable water flow in the liquid flow direction 23 along the forward rotation of the water pump motor, and the sample slice 17 enters through the pipe 10 under the impulsion of the water flow.
  • the screen 8 serves to prevent the sample slice 17 from entering the pipe 11 from the three-way sump 3.
  • Step S4 the first valve 1 is closed, the second valve 2 is opened, and the motor of the water pump 9 is reversed at a high speed to form a high-speed stable water flow in the direction 24, and the buffer liquid passes through the pipe 13, the water pump 9, the hose 11, and the filter from the water tank 15 in order.
  • the net 8, the three-way sump 3, the pipe 12 and the sub-head 4 enter a hole in the multi-empty plate 6, and the sample slicing 17 is driven by the high-speed steady flow of the liquid flow direction 24 along the reverse rotation of the water pump motor.
  • the through sump 3 enters a hole of the perforated plate 6 through the pipe 12 and the dispensing head 4, and the annular screen 5 on the dispensing head 4 serves to prevent the sample slice 17 from escaping from the hole of the multi-empty plate 6, redundant
  • the buffer is collected by the sump 7 and returned to the water tank 15 through the pipe 14.
  • Step S5 the three-axis robot arm 28 lifts the dispensing head 4, moves to the next hole of the perforated plate 6, and projects the dispensing head 4 into the hole, so that the annular filter 5 on the dispensing head 4 is The walls of the holes are in close contact.
  • step S6 it is determined whether to continue collecting slices. If the slices are collected continuously, steps S1 to S5 are repeated until the slice collection is completed; if the slice collection is completed, the next step is performed.
  • Step S7 stopping the slice collection.
  • the first valve 1, the second valve 2, the water pump 9 and the three-axis robot arm 28 are separately controlled by the industrial computer to realize the above working process, and the automatic control of the above electrical components by the industrial computer belongs to the common technology in the field. Means, no longer repeat them here.

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  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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Abstract

一种适用于振动切片的自动切片收集装置及方法，该装置包括：水槽（15），其内装有缓冲液（22）；振动切片机，其设于所述水槽（15）内；第一软管，其一端的端口对着振动切片机切除的切片（17），另一端连接带正反转电机的水泵（9）；所述第一管道上设有第一阀门（1），水泵（9）与第一管道之间设有滤网（8）；以及第二管道，其一端连接在所述第一管道的第一阀门（1）与水泵（9）之间，所述第二管道上设有第二阀门（2）。该收集装置能够实现振动切片过程中样本切片的自动收集，无需人工值守，提高工作效率。此外，该装置结构简单，易于和不同的振动切片机进行适配。

Description

适用于振动切片的自动切片收集装置及方法 [技术领域]
本发明涉及生物薄切片的制取设备，具体地指一种适用于振动切片的自动切片收集装置及方法。
[背景技术]
在现代生物研究和医学检查中，常常需要使用振动切片机将生物组织制作成一定厚度的薄切片，并进行染色、成像等进一步的处理和观察。振动切片过程一般在液体环境中进行，所生成的切片漂浮于液体中，需要手工挑出切片，并存放到多空板等收集容器中。在对样本进行大量、重复切片过程中，需要人工值守并进行重复的切片收集操作，效率低下，费时费力。因此，需要一种适用于振动切片的自动切片收集装置，在振动切片的过程中自动化的收集产生切片，并存贮到多孔板等收集容器中。
美国专利US20100093022中描述了一种通过传送带进行自动切片收集的装置，利用传动带贴附超薄切片机产生的树脂包埋的组织切片，并通过滚轮等结构收集并保存已贴附切片的传送带；不同于在空气中对树脂包埋样本进行的超薄切片，振动切片在液体环境中进行，所生成的柔软的组织切片无法贴附于传送带上，因此这种传送带式的切片收集装置不适用于振动切片方法。美国专利US20130019725中描述了一种通过控制水流进行自动切片收集的装置，利用水流将切片通过不同的水路收集至多孔板中，但其所设计的多条并行的水路结构重复且十分复杂，同时难以保证每次生成的切片准确的进入对应的管路中，影响切片收集效率。
综上所述，现有的适用于振动切片机的切片收集装置结构复杂，同时需对振动切片机进行大量的改造和适配。这些问题在实际应用中限制了此类方法的发展和应用，因此，研究一种简单、高效的适用于振动切片的自动切片收集装置很有必要。
[发明内容]
本发明目的在于克服上述现有技术的不足，提供一种适用于振动切片 的自动切片收集装置及方法，本发明能够实现对振动切片过程中产生的样本切片的自动收集，提高了切片和操作效率。
实现本发明目的采用的技术方案是一种适用于振动切片的自动切片收集装置，该装置包括：
水槽，其内装有缓冲液；
振动切片机，其设于所述水槽内；
第一管道，其一端的端口对着振动切片机切除的切片，另一端连接带正反转电机的水泵；所述第一管道上设有第一阀门，水泵与第一管道之间设有滤网；以及
第二管道，其一端连接在所述第一软管的第一阀门与水泵之间，所述第二管道上设有第二阀门。
在上述技术方案中，所述第二管道的另一端设有分装头，分装头的下方设有多孔板。
在上述技术方案中，所述分装头上套有环形滤网，环形滤网的直径为多孔板中孔的孔径。
在上述技术方案中，所述振动切片机包括刀片夹具及安装在刀片夹具上的刀片，所述刀片夹具上设有一个中空的通孔，所述通孔的一端对着刀片，另一端与所述第一管道连通。
进一步地，本发明装置还包括三轴机械臂，所述第一阀门、第二阀门和分装头分别设于三轴机械臂上。
在上述技术方案中，所述水泵通过第三管道与水槽连通。
更进一步地，本发明装置还包括集液槽，所述多孔板安装在所述集液槽中；所述集液槽通过第四管道与所述水槽连通。
此外，本发明还提供一种通过上述装置实现自动切片收集的方法，盖方法包括：
关闭第二阀门，开启第一阀门，控制水泵电机正转，振动切片机切除的切片随缓冲液流入第一管道内；滤网阻止切片从第一管道流出；
开启第一阀门，关闭第二阀门，控制水泵电机反转，第一管道内的切片随缓冲液从第二管道流至分装头；
控制三轴机械臂带动分装头对准多孔板的孔，所述切片流入孔中；
重复上述步骤，实现对样本的切片并将切片分装至多孔板的每一个孔内。
在上述方法中，分装头上的环形滤网阻止切片从多空板的孔中逸出；从孔中溢出的缓冲液由集液槽收集并经过第四管道回流至水槽中。
在上述方法中，水泵电机正转时，抽出的缓冲液通过第三软管抽入至水槽。
在上述方法中，水泵电机正转时，抽出的缓冲液通过第三管道抽入至水槽。
本发明具有以下优点：
1、实现了振动切片过程中样本切片的自动收集，无需人工值守，提高工作效率。
2、结构简单，易于和不同的振动切片机进行适配。
[附图说明]
图1为本发明适用于振动切片的自动切片收集装置的一种优选实施例结构示意图。
图2为本发明适用于振动切片的自动切片收集装置的一种优选实施例三维结构示意图。
图3为实施例所用振动切片机的结构示意图。
图4为使用本发明装置进行自动切片收集的工作流程图。
图中标号：1-第一阀门，2-第二阀门，3-三通贮片器，4-分装头，5-环形滤网，6-多孔板，7-集液槽，8-滤网，9-水泵，10-管道，11-管道，12-管道，13-管道，14-管道，15-水槽，16-生物样本，17-样本切片，18-刀片，19-刀片夹具，20-通孔，21-通孔上端，22-缓冲液，23-水泵电机正转时液体流动方向，24-水泵电机反转时液体流动方向，25-螺孔，26-螺孔，27-通孔的膨胀端，28-三轴机械臂。
[具体实施方式]
下面结合附图和具体实施例对本发明作进一步的详细说明。
本实施例适用于振动切片的自动切片收集装置，其结构如图1所示，该装置包括水槽15，水槽15内装有缓冲液22，生物样本16和振动切片机均位于缓冲液22中，以保证切片过程在液体环境中进行。本实施例所用振动切片机的结构如图2所示，包括刀片夹具19和设于刀片夹具19上的刀片18，螺母拧进螺孔(25，26)中，将刀片18紧固，刀片夹具19上设有一个通孔20，通孔20的下端对着刀片18的刀刃处。
本实施例适用于振动切片的自动切片收集装置还包括三通贮片器3、管道10、管道11、管道12、管道13、管道14和多孔板6，其中，
管道10的一端与三通贮片器3的第一通道连接，另一端与通孔的上端20连接，通孔20的侧边设有膨胀端27，膨胀端27便于与管道10紧固的连接为一体。管道10上设有第一阀门1，第一阀门1用于控制管道10的通断，第一阀门1可以采用电磁夹管阀。
管道11的一端与三通贮片器3的第二通道连接，另一端连接有分装头4，多孔板6位于分装头4的下方，分装头4上套有环形滤网5，环形滤网5的直径与多孔板6中孔的孔径相同，当分装头4伸入多孔板6的孔内后，环形滤网5能与孔四周的内壁接触。软管上设有第二阀门2，第二阀门1用于控制软管10的通断，第二阀门1可以采用电磁夹管阀。多孔板6安放在集液槽7中，集液槽7通过管道14与水槽15连通。
软管13的一端与三通贮片器3的第三通道连接，且连接处设有滤网8，另一端设有带正反转电机的水泵9。水泵9还通过软管13与水槽15连通。
为了实现自动、快速、持续不间断地对切片的收集，本实施例适用于振动切片的自动切片收集装置还包括三轴机械臂28，第一阀门1、第二阀门2、三通贮片器3和分装头4设于三轴机械臂28上，如图3所示。
本实施例自动切片收集装置中各部件的说明如下：第一阀门1和第二阀门2为电磁夹管阀；三通贮片器3和分装头4为塑料加工件；环形滤网5和滤网8由不锈钢滤网加工而成；多空板6为聚苯乙烯材质的24孔板，集液槽7和水槽15为铝制加工件；水泵9为直流无刷电机驱动的齿轮泵，流 量调节范围为50～1000ml/min；管道10、管道11、管道12、管道13、管道14均为硅胶管，内径为6.4mm，外径为9mm；刀片18采用Electron Microscopy Sciences公司的氧化锆刀片，刀片夹具19为不锈钢加工件；三轴机械臂28为Yamaha公司生产的型号为T6L-T5L-T4L的三轴机械臂。
图4为本实施例适用于振动切片的自动切片收集装置的工作流程图，下面结合本实施例装置的各部件，对实施例装置工作过程的详细说明如下：
步骤S1、将第二阀门2关闭，第一阀门1开启，控制水泵9低速正转，形成沿水泵电机正转时液体流动方向23的低速稳定水流，缓冲液22从通孔20下端的开口处的进入通孔中，然后从通孔20的上端依次经过管道10、三通贮片器3、滤网8、管道11、水泵9和管道13后回流至水槽15中。
步骤S2、振动切片机带动刀片18开始对生物样本16进行切片，切片过程中生成的样本切片17，在水泵电机正转时液体流动方向23的低速稳定水流的推动下，从通孔20下端开口进入通孔中，水流速度较低以避免样本切片17被撕裂。
步骤S3、当刀片18完成对生物样本16整个断面的切片后，水泵9提高转速，形成沿水泵电机正转时液体流动方向23的高速稳定水流，样本切片17在水流的推动下通过管道10进入三通贮片器3中，滤网8用于阻止样本切片17从三通贮片器3进入到管道11中。
步骤S4、第一阀门1关闭，第二阀门2开启，水泵9的电机高速反转，形成沿方向24的高速稳定水流，缓冲液从水槽15依次经过管道13、水泵9、软管11、滤网8、三通贮片器3、管道12和分装头4进入多空板6的一个孔中，样本切片17在沿水泵电机反转时液体流动方向24的高速稳定水流的推动下从三通贮片器3经过管道12和分装头4进入到多孔板6的一个孔中，分装头4上的环形滤网5用于阻止样本切片17从多空板6的孔中逸出，多余的缓冲液由集液槽7收集并经过管道14回流至水槽15中。
步骤S5、三轴机械臂28将分装头4抬起，移动至多孔板6的下一个孔处并将分装头4伸入该孔中，使分装头4上的环形滤网5与孔壁紧密接触。
步骤S6、判断是否继续收集切片，若继续收集切片则重复步骤S1至S5直至完成切片收集；若已完成切片收集，则执行下一步骤。
步骤S7、停止切片收集。
为了实现上述过程的自动化，通过工控机分别控制第一阀门1、第二阀门2、水泵9以及三轴机械臂28实现上述工作过程，通过工控机控制上述电气元件自动工作属于本领域所常用技术手段，此处不再赘述。

Claims (10)

  1. 一种适用于振动切片的自动切片收集装置，其特征在于，包括：
    水槽，其内装有缓冲液；
    振动切片机，其设于所述水槽内；
    第一管道，其一端的端口对着振动切片机切除的切片，另一端连接带正反转电机的水泵；所述第一管道上设有第一阀门，水泵与第一管道之间设有滤网；以及
    第二管道，其一端连接在所述第一软管的第一阀门与水泵之间，所述第二管道上设有第二阀门。
  2. 根据权利要求1所述适用于振动切片的自动切片收集装置，其特征在于：所述第二管道的另一端设有分装头，分装头的下方设有多孔板。
  3. 根据权利要求2所述适用于振动切片的自动切片收集装置，其特征在于：所述分装头上套有环形滤网，环形滤网的直径为多孔板中孔的孔径。
  4. 根据权利要求1～3任一项所述适用于振动切片的自动切片收集装置，其特征在于：所述振动切片机包括刀片夹具及安装在刀片夹具上的刀片，所述刀片夹具上设有一个中空的通孔，所述通孔的一端对着刀片，另一端与所述第一管道连通。
  5. 根据权利要求4所述适用于振动切片的自动切片收集装置，其特征在于：还包括三轴机械臂，所述第一阀门、第二阀门和分装头分别设于三轴机械臂上。
  6. 根据权利要求5所述适用于振动切片的自动切片收集装置，其特征在于：所述水泵通过第三管道与水槽连通。
  7. 根据权利要求6所述适用于振动切片的自动切片收集装置，其特征在于：包括集液槽，所述多孔板安装在所述集液槽中；所述集液槽通过第四管道与所述水槽连通。
  8. 一种通过权利要求1所述装置实现自动切片收集的方法，其特征在于，包括：
    关闭第二阀门，开启第一阀门，控制水泵电机正转，振动切片机切除的切片随缓冲液流入第一管道内；滤网阻止切片从第一管道流出；
    开启第一阀门，关闭第二阀门，控制水泵电机反转，第一管道内的切片随缓冲液从第二管道流至分装头；
    控制三轴机械臂带动分装头对准多孔板的孔，所述切片流入孔中；
    重复上述步骤，实现对样本的切片并将切片分装至多孔板的每一个孔内。
  9. 根据权利要求8所述实现自动切片收集的方法，其特征在于：分装头上的环形滤网阻止切片从多空板的孔中逸出；从孔中溢出的缓冲液由集液槽收集并经过第四管道回流至水槽中。
  10. 根据权利要求9所述实现自动切片收集的方法，其特征在于：水泵电机正转时，抽出的缓冲液通过第三管道抽入至水槽。
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