WO2017071197A1 - Control agent for preventing and treating cutworm-damage - Google Patents
Control agent for preventing and treating cutworm-damage Download PDFInfo
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- WO2017071197A1 WO2017071197A1 PCT/CN2016/083493 CN2016083493W WO2017071197A1 WO 2017071197 A1 WO2017071197 A1 WO 2017071197A1 CN 2016083493 W CN2016083493 W CN 2016083493W WO 2017071197 A1 WO2017071197 A1 WO 2017071197A1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
Definitions
- the invention relates to a combined control agent for controlling tiger insect pests, belonging to the technical field of plant pest control.
- the genus Diptera is a genus of the genus Astragalus and the genus Noctuidae. It belongs to the multi-feeding crop pests.
- the larvae bite the crops by larvae.
- the larvae bite off the stems near the ground, causing the whole plant to die and causing the seedlings to be broken.
- Hazardous crops include cotton, corn, sorghum, wheat, hemp, oysters, tobacco, beets, rapeseed, vegetables, melons, medicinal plants, pastures, forest nurseries, and a variety of omnivorous plants that harm crops.
- Common species are small tigers, yellow tigers, earth tigers, white tigers and police tigers.
- the current common methods of prevention and control are physical and chemical pesticides.
- the physical control method uses the phototaxis of Noctuidae. Purple black light with sweet and sour liquid for adult trapping, the main role is to reduce the number of adults, the common use of chemical pesticides for insecticide, the main components of chemical pesticides are organophosphate insecticides such as trichlorfon, Xin Products such as sulphur phosphorus, chlorpyrifos, etc., pyrethroid insecticides such as deltamethrin, pyrethroid emulsifiable concentrate, etc., the use of chemical pesticides mentioned above causes soil and groundwater pollution to become more and more serious. The problem of endangering the environment and food safety is not uncommon.
- the object of the present invention is to use the plant source and the biological source to formulate the functions of killing and repelling according to the feeding mode, physiological structure and migration characteristics of the tiger, thereby achieving the harm of controlling the tiger.
- a combined control agent for controlling tiger worms which is characterized by comprising the following components by weight: 2-8% chitosan, 3-9% wormwood oil, Sophora flavescens Alkali 2-5%, the balance is added to 100% of Trichoderma viride liquid;
- the green Trichoderma liquid is prepared by inoculating the Trichoderma viride enrichment group to enrich the spore culture of Trichoderma viride;
- the sporulation medium consists of the following components by weight: 4% eucalyptus, 1% corn flour, 0.003% copper hydroxide, 0.05% magnesium sulfate, 0.01% manganese sulfate, 0.003% ferrous sulfate, and the balance is water plus To 100%, Trichoderma viride is inoculated with 0.1% of sporulation medium;
- the invention preferentially gives the weight ratio of the best combination components: 5% chitosan, 6% wormwood oil, 3% matrine, and the balance is Trichoderma viride liquid;
- the preparation method comprises the following steps: after the sporulation medium is prepared, the green Trichoderma inoculation is carried out, and the sporulation culture is carried out for 7 days, and the residue is further filtered to obtain green wood. Mold, sequentially add chitosan, wormwood oil, matrine to the bacterial liquid to stir evenly, then obtain a combination of prevention and control agent for tiger insect pests;
- the material of the present invention is derived from a plant source and a biological source, and is easily degraded by the soil into nutrients required by the plant after application, without causing residue;
- the invention has the effect of repelling larvae after being applied to the soil
- a protective film can be formed around the root of the crop
- Figure 1 is a process flow diagram of a combined control agent for controlling tiger worms of the present invention
- Case 1 A farm in Zhangjiagang, Jiangsu province, planted 120 mu of corn. In April 2015, it found that corn seedlings fell 13%, checked the stems of fallen corn seedlings, wounded with insect bites, and dug the soil near the plants. It was found that the larvae and cockroaches of the larvae were irrigated with 1000 times of the control agent of the tiger worm pest control of the present invention, and the rooting was continued once every 3 days after the rooting, and the corn seedlings around the fallen corn were examined on the 7th day. The soil of the larvae of the tiger was found in the soil, and the insects were inspected in May, and no other corn was bitten;
- Case 2 A grower in Longyan, Fujian, planted 50 mu of tobacco. In late April 2015, the phenomenon of death of seedlings was found, accounting for 25% of the whole garden. The soil was dug up and found to be a tiger hazard. One of the inventions was used. The roots of the tiger insect pest control agent 1000 times were irrigated, and the soil was sprayed with 500 times liquid on the 3rd day after rooting. On the 7th day, the soil of the damaged plant was examined and the corpse of the tiger larva was found until 6 No insects were found during the month of harvest.
- the method of using root irrigation or soil spraying can control the insect condition, which is a green non-toxic ground tiger prevention solution worth promoting.
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The present invention discloses a combined control agent for preventing and treating cutworm-damage, comprising the following components by weight percentage: chitosan 2-8%, wormwood oil 3-9%, matrine 2-5%, and trichoderma viride culture solution as the balance added to 100%; wherein the trichoderma viride solution is prepared after a trichoderma viride-enriched medium is inoculated with trichoderma viride-enriched sporulation culture; the sporulation culture medium is composed of the following components by weight percentages: 4% of camphor clastic, 1% of corn flour, 0.003% of copper hydroxide, 0.05% of magnesium sulfate, 0.01% of manganese sulfate, 0.003% of ferrous sulfate, and water as the balance added to 100%, and the trichoderma viride is inoculated with 0.1% of the sporulation culture medium. The present invention has the effects of killing and repelling the damage of cutworms to crops, inducing the crop to generate self-protection, promoting the robust growth of crops, and improving crops quality.
Description
本发明涉及一种防治地老虎虫害组合防治剂，属于植物虫害防治的技术领域。The invention relates to a combined control agent for controlling tiger insect pests, belonging to the technical field of plant pest control.
地老虎属鳞赤目、夜蛾科，属于多食性的作物害虫，主要以幼虫咬食危害作物，幼虫将近地面的茎部咬断，使整株死亡、造成缺苗断垅。危害作物有棉、玉米、高粱、麦类、麻类、苜宿、烟草、甜菜、油菜、蔬菜、瓜类、药用植物、牧草、林木苗圃，为多种杂食性植物，危害作物的地老虎常见的种类有小地老虎、黄地老虎、大地老虎、白边地老虎和警纹地老虎，目前常见的防治方法有物理性、化学农药的防治方法，物理防治方法以夜蛾的趋光性，采用紫黑灯配合糖醋液进行成虫的诱杀，主要的作用是降低成虫的群体数量，常见的是用化学农药进行杀虫，化学农药主要的成分为有机磷类杀虫剂如敌百虫、辛硫磷、杀螟磷硫等制成品，菊酯类杀虫剂如溴氰菊酯、菊酯乳油等制品，上面所述的化学农药使用后造成土壤、地下水污染的程度越来越严重，危害环境与食品安全的问题，屡见不鲜，在无公害、绿色安全的食品要求下，上述的化学农药会逐渐地退出市场，如何依据地老虎的生活习性、组织结构，寻找植物源、生物源与微量元素调配出具有环境和谐、无毒无残留的高效方法，是现在植保技术人员需要深入研究探讨的一个重要的亟需突破的技术难题。The genus Diptera is a genus of the genus Astragalus and the genus Noctuidae. It belongs to the multi-feeding crop pests. The larvae bite the crops by larvae. The larvae bite off the stems near the ground, causing the whole plant to die and causing the seedlings to be broken. Hazardous crops include cotton, corn, sorghum, wheat, hemp, oysters, tobacco, beets, rapeseed, vegetables, melons, medicinal plants, pastures, forest nurseries, and a variety of omnivorous plants that harm crops. Common species are small tigers, yellow tigers, earth tigers, white tigers and police tigers. The current common methods of prevention and control are physical and chemical pesticides. The physical control method uses the phototaxis of Noctuidae. Purple black light with sweet and sour liquid for adult trapping, the main role is to reduce the number of adults, the common use of chemical pesticides for insecticide, the main components of chemical pesticides are organophosphate insecticides such as trichlorfon, Xin Products such as sulphur phosphorus, chlorpyrifos, etc., pyrethroid insecticides such as deltamethrin, pyrethroid emulsifiable concentrate, etc., the use of chemical pesticides mentioned above causes soil and groundwater pollution to become more and more serious. The problem of endangering the environment and food safety is not uncommon. Under the pollution-free, green and safe food requirements, the above-mentioned chemical pesticides will gradually withdraw from the market, and how to rely on the life of the tigers , Organizational structure, looking for plant sources, and biogenic trace element has formulated a harmonious environment, an efficient method of residue-free, non-toxic, plant protection technicians need now is to explore in depth study of an important breakthrough in urgent need of technical problems.
发明内容Summary of the invention
为解决上述的技术难题，本发明的目的是依据地老虎的取食方式、生理结构、迁移的特性，采用植物源、生物源调配出具有灭杀与驱避的的功能，达到防治地老虎危害经济作物的效果。In order to solve the above technical problems, the object of the present invention is to use the plant source and the biological source to formulate the functions of killing and repelling according to the feeding mode, physiological structure and migration characteristics of the tiger, thereby achieving the harm of controlling the tiger. The effect of cash crops.
为实现上述的目的，提供一种防治地老虎虫害组合防治剂，其所述的特征在于包括以下的重量百分比的组分：壳聚糖2-8％、艾草油3-9％、苦参碱2-5％，余量为绿色木霉菌养液加至100％；In order to achieve the above object, a combined control agent for controlling tiger worms is provided, which is characterized by comprising the following components by weight: 2-8% chitosan, 3-9% wormwood oil, Sophora flavescens Alkali 2-5%, the balance is added to 100% of Trichoderma viride liquid;
其中绿色木霉菌液是由绿色木霉富集基接种绿色木霉富集产孢培养后所制取；The green Trichoderma liquid is prepared by inoculating the Trichoderma viride enrichment group to enrich the spore culture of Trichoderma viride;
产孢培养基由包括以下重量百分比的组分：樟木屑4％、玉米粉1％、氢氧化铜0.003％、硫酸镁0.05％、硫酸锰0.01％、硫酸亚铁0.003％，余量为水加至100％，绿色木霉以产孢培养基的0.1％接种；The sporulation medium consists of the following components by weight: 4% eucalyptus, 1% corn flour, 0.003% copper hydroxide, 0.05% magnesium sulfate, 0.01% manganese sulfate, 0.003% ferrous sulfate, and the balance is water plus To 100%, Trichoderma viride is inoculated with 0.1% of sporulation medium;
本发明优先出最佳组合成分的重量百为分比：壳聚糖5％、艾草油6％、苦参碱3％、余量为苏绿色木霉菌液；
The invention preferentially gives the weight ratio of the best combination components: 5% chitosan, 6% wormwood oil, 3% matrine, and the balance is Trichoderma viride liquid;
为实现本发明的一种防治地老虎虫害组合防治剂，其制备方法：将产孢培养基调配完成后，接种绿色木霉，进行富集产孢培养7 d，进一步过滤残渣后得将绿色木霉菌，依序加入壳聚糖、艾草油、苦参碱加入菌液进行搅拌均匀后即得一种防治地老虎虫害组合防治剂；In order to realize the prevention and control agent for controlling the tiger insect pest of the invention, the preparation method comprises the following steps: after the sporulation medium is prepared, the green Trichoderma inoculation is carried out, and the sporulation culture is carried out for 7 days, and the residue is further filtered to obtain green wood. Mold, sequentially add chitosan, wormwood oil, matrine to the bacterial liquid to stir evenly, then obtain a combination of prevention and control agent for tiger insect pests;
本发明的优点：Advantages of the invention:
1、本发明的材料来自植物源与生物源，施用后容易由土壤降解成植物需要的营养，不产生残留；1. The material of the present invention is derived from a plant source and a biological source, and is easily degraded by the soil into nutrients required by the plant after application, without causing residue;
2、本发明施入土壤后具有驱避幼虫的功效；2. The invention has the effect of repelling larvae after being applied to the soil;
3、本发明施入土壤后，能在作物的根系周围形成保护膜；3. After the soil of the invention is applied to the soil, a protective film can be formed around the root of the crop;
3、本发明的工艺简单，与目前单用苦参碱，防治效果增加70％。3. The process of the invention is simple, and the control effect is increased by 70% with the current use of matrine alone.
图1为本发明的一种防治地老虎虫害组合防治剂的工艺流程图Figure 1 is a process flow diagram of a combined control agent for controlling tiger worms of the present invention
下面配合实际的使用案例说明本发明的特性The following describes the characteristics of the present invention in conjunction with actual use cases.
1、案例一：江苏张家港某农场，种植玉米120亩地，在2015年4月份发现玉米苗倒伏13％，检查倒伏的玉米苗的茎部，有虫咬的伤口，挖开植株附近的土壤，发现有地老虎的幼虫与蛹，使用本发明的一种防治地老虎虫害组合防治剂1000倍液进行灌根，在灌根后3天继续灌根一次，第7天检查已倒伏的玉米苗周围的土壤，发现地老虎的幼虫的尸体，在5月份检查虫情，未见其他的玉米被咬断的情况；1. Case 1: A farm in Zhangjiagang, Jiangsu Province, planted 120 mu of corn. In April 2015, it found that corn seedlings fell 13%, checked the stems of fallen corn seedlings, wounded with insect bites, and dug the soil near the plants. It was found that the larvae and cockroaches of the larvae were irrigated with 1000 times of the control agent of the tiger worm pest control of the present invention, and the rooting was continued once every 3 days after the rooting, and the corn seedlings around the fallen corn were examined on the 7th day. The soil of the larvae of the tiger was found in the soil, and the insects were inspected in May, and no other corn was bitten;
2、案例二：福建龙岩某种植户，种植烟草50亩，于2015年4月下旬发现苗倒死亡的现象，占整园的25％，挖开土壤发现为地老虎危害，使用本发明的一种防治地老虎虫害组合防治剂1000倍液进行灌根，在灌根后的第3天用500倍液喷施土壤，第7天检查受害植株的土壤附近，发现地老虎幼虫的尸体，直至6月份采收时未发现虫情发生。2. Case 2: A grower in Longyan, Fujian, planted 50 mu of tobacco. In late April 2015, the phenomenon of death of seedlings was found, accounting for 25% of the whole garden. The soil was dug up and found to be a tiger hazard. One of the inventions was used. The roots of the tiger insect pest control agent 1000 times were irrigated, and the soil was sprayed with 500 times liquid on the 3rd day after rooting. On the 7th day, the soil of the damaged plant was examined and the corpse of the tiger larva was found until 6 No insects were found during the month of harvest.
由以上实际在大田的应用上，当地老虎危害时，使用灌根或土壤喷施的方法均可控制虫情，是值得推广的一种绿色无毒的地老虎防治的解决方案。From the above actual application in Daejeon, when the local tiger is harmed, the method of using root irrigation or soil spraying can control the insect condition, which is a green non-toxic ground tiger prevention solution worth promoting.
虽然本发明已以较佳实施例揭示如上，然其并非用以限定本发明，任何本领域技术人员，在不脱离本发明的精神和范围内，当可作些许的修改和完善，因此本发明的保护范围当以权利要求书所界定的为准。
While the present invention has been described in its preferred embodiments, the present invention is not intended to be limited thereto, and the present invention may be modified and improved without departing from the spirit and scope of the invention. The scope of protection is defined by the terms of the claims.
Claims (2)
- 一种防治地老虎虫害组合防治剂，其所述特征在于包括以下重量百分比的组分：壳聚糖2-8％、艾草油3-9％、苦参碱2-5％，余量为绿色木霉菌养液加至100％；其中绿色木霉菌液是由绿色木霉富集基接种绿色木霉富集产孢培养后所制取；产孢培养基由以下重量百分比的组分：樟木屑4％、玉米粉1％、氢氧化铜0.003％、硫酸镁0.05％、硫酸锰0.01％、硫酸亚铁0.003％，余量为水加至100％，绿色木霉以产孢培养基的0.1％接种。A combined control agent for controlling tiger worms, characterized in that it comprises the following components by weight percentage: 2-8% chitosan, 3-9% wormwood oil, 2-5% matrine, and the balance is The green Trichoderma solution is added to 100%; wherein the Trichoderma viride liquid is prepared by inoculating the Trichoderma viride enrichment group to enrich the spore culture; the sporulation medium is composed of the following weight percentage components: Wood chips 4%, corn flour 1%, copper hydroxide 0.003%, magnesium sulfate 0.05%, manganese sulfate 0.01%, ferrous sulfate 0.003%, the balance is water added to 100%, Trichoderma viride is 0.1% of sporulation medium % vaccination.
- 如权利要求1一种防治地老虎虫害组合防治剂，其特征在于优先出最佳组合成分的重量百为分比：壳聚糖5％、艾草油6％、苦参碱3％、余量为苏绿色木霉菌液。 A combination control agent for controlling tiger worms according to claim 1, characterized in that the weight ratio of the best combination components is preferentially divided: 5% of chitosan, 6% of wormwood oil, 3% of matrine, and the balance It is a green Trichoderma liquid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510701957.3A CN105211108A (en) | 2015-10-27 | 2015-10-27 | A kind of control cutworm insect pest combination control agent |
| CN201510701957.3 | 2015-10-27 |
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| CN105594751B (en) * | 2016-01-28 | 2018-10-23 | 黄河三角洲京博化工研究院有限公司 | A kind of reesei spores preparation and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745629A (en) * | 2005-10-19 | 2006-03-15 | 中国科学院海洋研究所 | A kind of agricultural bacteriocide |
| CN102578155A (en) * | 2012-01-11 | 2012-07-18 | 王广富 | Peanut grub prevention and control agent and production method thereof |
| CN104304323A (en) * | 2014-09-18 | 2015-01-28 | 山东省果树研究所 | Method for controlling black cutworm by combining entomopathogenic nematodes with matrine |
| CN105211108A (en) * | 2015-10-27 | 2016-01-06 | 苏州科大微龙农业科技有限公司 | A kind of control cutworm insect pest combination control agent |
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| CN104738079A (en) * | 2013-12-26 | 2015-07-01 | 中国中化股份有限公司 | Binary insecticide composition and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745629A (en) * | 2005-10-19 | 2006-03-15 | 中国科学院海洋研究所 | A kind of agricultural bacteriocide |
| CN102578155A (en) * | 2012-01-11 | 2012-07-18 | 王广富 | Peanut grub prevention and control agent and production method thereof |
| CN104304323A (en) * | 2014-09-18 | 2015-01-28 | 山东省果树研究所 | Method for controlling black cutworm by combining entomopathogenic nematodes with matrine |
| CN105211108A (en) * | 2015-10-27 | 2016-01-06 | 苏州科大微龙农业科技有限公司 | A kind of control cutworm insect pest combination control agent |
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