WO2017012573A1 - 一株短小芽孢杆菌及其保育仔猪用复合微生态制剂 - Google Patents

一株短小芽孢杆菌及其保育仔猪用复合微生态制剂 Download PDF

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WO2017012573A1
WO2017012573A1 PCT/CN2016/090899 CN2016090899W WO2017012573A1 WO 2017012573 A1 WO2017012573 A1 WO 2017012573A1 CN 2016090899 W CN2016090899 W CN 2016090899W WO 2017012573 A1 WO2017012573 A1 WO 2017012573A1
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bacillus subtilis
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张梁
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Jiangnan University
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • the invention relates to the field of microbial technology, in particular to a strain of Bacillus brevis and its use in the conservation of piglets.
  • the microecological preparation has the functions of regulating intestinal microflora balance, proliferating beneficial bacteria, inhibiting harmful bacteria, lowering intestinal pH value, increasing digestive enzyme activity, nutrient digestibility and immunity, and microecological preparation in piglet breeding.
  • the application reports are increasing.
  • CN 101703136B reports the application of the Lactobacillus plantarum preparation in the prevention and treatment of piglet diarrhea, the efficiency of piglet diarrhea is more than 90%, and the cure rate is over 80%.
  • CN 103255080B reports the use of Bacillus coagulans HM-09 in the preparation of feed for improving the performance of piglets and diarrhea, which can reduce the incidence of diarrhea in weaned piglets, thereby reducing the incidence of intestinal diseases and improving feed digestion.
  • CN 103205373B reported a Clostridium butyricum strain for suckling pigs, which can antagonize animal pathogens in the intestinal tract of suckling pigs, maintain and regulate micro-ecological balance, enhance immune function, produce starch plum and vitamins, and promote animal feed. Digestive absorption and nutritional effects.
  • microecological preparations have a slow onset of action, and they need to be used consistently in livestock and poultry farming to receive good results. Therefore, the development of a microecological preparation that can not only long-term health care, but also quickly solve the problems of piglet diarrhea, diarrhea, etc. is particularly necessary for the stable production of nursery piglets.
  • the applicant provides a composite microecological preparation for Bacillus pumilus and its nursery piglets.
  • the invention can quickly solve the problems of piglet diarrhea, squatting, etc. Improve the health of nursery pigs during long-term use.
  • a strain of Bacillus pumilus ZS008 was deposited with the China Center for Type Culture Collection under the accession number CCTCC No. M2015451 and the date of deposit was July 13, 2015.
  • a compound Bacillus subtilis preparation for nursery pigs is prepared by combining the following strains into a composite fungus: Bacillus pumilus CCTCC No. M2015451, Bacillus coagulans CCTCC No. M2015273, Bacillus subtilis CCTCC No. M 2011443, lichens spore Bacillus CCTCC No. M 2012458.
  • the effective viable cell count of the composite Bacillus subtilis preparation is 1.4 ⁇ 10 8 -7.0 ⁇ 10 10 cfu/g, and the composition of each strain is as follows:
  • Bacillus coagulans is Bacillus coagulans ZS007 and is deposited with the China Center for Type Culture Collection under the accession number CCTCC NO: M 2015273.
  • the application of the composite Bacillus subtilis preparation for the nursery piglets is to improve the performance of the nursery piglets.
  • the amount used is 1.0 to 5.0 g/head ⁇ day.
  • the single bacterial powder of Bacillus subtilis CCTCC No.M 2011443 and Bacillus licheniformis CCTCC No.M 2012458 was purchased from Taizhou Green Shengyuan Biotechnology Co., Ltd., and its product specifications were all 5.0 ⁇ 10 10 cfu/g.
  • the health level of the nursery pigs can be improved, so as to improve the production capacity of the nursery pigs.
  • the strain numbered ZS008 was finally obtained and identified as Bacillus pumilus.
  • the strain is deposited in the China Center for Type Culture Collection under the accession number CCTCC No.M2015451 and the deposit date is July 13, 2015.
  • strain numbered ZS-007 was finally obtained and identified as Bacillus coagulans.
  • ZS-007 was deposited with the China Type Culture Collection on May 3, 2015 under the accession number CCTCC M 2015273.
  • the composite microecological preparation is compounded by Bacillus pumilus CCTCC No. M2015451, Bacillus coagulans CCTCC No. M 2015273, Bacillus subtilis CCTCC No. M 2011443, and Bacillus licheniformis CCTCC No. M 2012458.
  • the single bacterial powder of Bacillus subtilis CCTCC No. M 2011443 and Bacillus licheniformis CCTCC No. M 2012458 was purchased from Taizhou Green Shengyuan Biotechnology Co., Ltd., and its product specifications were all 5.0 ⁇ 10 10 cfu/g.
  • microecological preparation products prepared in Examples 1 to 3 the contents of each microorganism are as follows:
  • anhydrous glucose is added as a carrier to supplement 100% of the total weight (Bacillus bacillus is used as an active ingredient, and the total weight of the product is satisfied to reach the final viable cell count of 4.0 ⁇ 10 8 cfu. /g, mix well and set aside.
  • the processing should ensure the cleanliness of the operating environment and avoid contamination. Control the ambient humidity below 70%, the moisture content of each raw material is less than 13%, the product is stored at room temperature for 24 months after being made, the shelf life at -18 °C can reach more than 60 months, and the number of viable bacteria in the shelf life is stable.
  • the piglets were divided into three groups: A, B and C.
  • Group A was the control group
  • B test group 1 and C group were the test group 2
  • the basic diet of each group is the same (Zhengda full price), and the nutritional level is consistent.
  • Group A did not add any enzyme preparation, and the group B basic diet was added with a composite microecological preparation containing Bacillus pumilus (Example 1), Group C was the same as Group B, but did not contain Bacillus pumilus; the amount of microecological preparation was The amount used is 1.0 g / head ⁇ day.
  • the piglets were 21 days old, weaned at an average weight of 6.5 kg, pre-tested for 7 days, and fed for 30 days. A breeding cooperative in Nanjing.
  • the piglets After the piglets have diarrhea, they are treated with microecological preparations at a dose of 5g per pig. After the product was stirred with 10 mL of cold boiled water, the syringe was drawn into the syringe and filled with a syringe along the mouth of the piglet.
  • the microecological preparation product contains Bacillus brevis
  • the piglets improved after 4-6 hrs, and the diarrhea was terminated within 24 hr, and the appetite was restored.
  • the microecological preparation product does not contain Bacillus brevis, the piglets turn better after 12 hours; the treatment is consolidated once, and the diarrhea is terminated in about 2 days to restore appetite.

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Abstract

提供了一株短小芽孢杆菌(Bacillus pumilus)ZS008，保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO.M2015451，保藏日期为2015年7月13日。还提供了一种保育仔猪用复合微生态芽孢杆菌制剂，其由以下菌种组合配制而成：短小芽孢杆菌CCTCC No.M2015451、凝结芽孢杆菌CCTCC No.M2015273、枯草芽孢杆菌CCTCC No.M 2011443、地衣芽孢杆菌CCTCC No.M 2012458。

Description

一株短小芽孢杆菌及其保育仔猪用复合微生态制剂 技术领域
本发明涉及微生物技术领域，尤其是涉及一株短小芽孢杆菌，及其在有益于保育仔猪方面的应用。
背景技术
保育仔猪，简称断奶仔猪，此阶段的猪相当脆弱，母源抗体消失，新的免疫系统还没有建立，再加上生长速度快、应激因素多等原因，很容易发生各种疾病甚至出现大面积死亡。抗生素、化学药品虽然能在较短时间内解决出现的问题，但由此引发的药物抗性和药物残留问题，有可能会对仔猪后期生长产生影响。如何提高保育仔猪健康状况、成活率、整齐度、充分发挥生长后效性，是每个养殖户共同关心的问题。
微生态制剂具有调控动物肠道微生物平衡、增殖有益菌、抑制有害菌、降低肠道pH值、提高消化道酶活性、营养物质消化率和机体免疫力等作用，关于微生态制剂在仔猪养殖中的应用报道日益增多。CN 101703136B报道了植物乳杆菌活菌制剂在防治仔猪拉稀上的应用，仔猪拉稀有效率在90％以上，治愈率80％以上。CN 103255080B报道了凝结芽孢杆菌(Bacillus coagulans HM-09)在制备改善乳仔猪生产性能和腹泻的饲料中的应用，可以减少断奶仔猪腹泻的发生率，进而减少肠源性疾病的发生，提高饲料消化率，提高仔猪饲料利用率。CN 103205373B报道了一株用于乳仔猪养殖的丁酸梭菌，可在乳仔猪肠道内拮抗动物病原菌，维持和调节微生态平衡，增强机体免疫功能，并产生淀粉梅、维生素，促进动物对饲料的消化吸收和营养作用。
一般来说，微生态制剂起效慢，畜禽养殖过程中需要坚持使用才能收到很好的效果。因此，开发一种既能长效保健用、又能快速解决仔猪拉稀、下痢等问题的微生态制剂，对于实现保育仔猪平稳生产显得尤为必要。
发明内容
针对现有技术存在的上述问题，本申请人提供了一株短小芽孢杆菌及其保育仔猪用复合微生态制剂。本发明既能快速解决仔猪拉稀、下痢等问题，又能在 长期使用过程中提升保育仔猪的健康水平。
本发明的技术方案如下：
一株短小芽孢杆菌(Bacillus pumilus)ZS008，保藏于中国典型培养物保藏中心，保藏编号为CCTCC No.M2015451，保藏日期为2015年7月13日。
一种保育仔猪用复合微生态芽孢杆菌制剂，由以下菌种组合配制成为复合菌剂：短小芽孢杆菌CCTCC No.M2015451、凝结芽孢杆菌CCTCC No.M2015273、枯草芽孢杆菌CCTCC No.M 2011443、地衣芽孢杆菌CCTCC No.M 2012458。
所述复合微生态芽孢杆菌制剂的有效活菌数为1.4×108-7.0×1010cfu/g，各菌种的组成成分如下：
Figure PCTCN2016090899-appb-000001
所述凝结芽孢杆菌为凝结芽孢杆菌(Bacillus coagulans)ZS007，保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO:M 2015273。
所述的保育仔猪用复合微生态芽孢杆菌制剂的应用在于提升保育仔猪的生产性能。使用量为1.0～5.0g/头·天。
枯草芽孢杆菌CCTCC No.M 2011443、地衣芽孢杆菌CCTCC No.M 2012458的单菌粉购自泰州绿圣元生物科技有限公司，其产品规格均为5.0×1010cfu/g
本发明有益的技术效果在于：
(1)能快速解决仔猪拉稀、下痢等问题；
(2)长期使用后，可以提升保育仔猪的健康水平，从而达到提升保育仔猪生产能力的效果。
具体实施方式
1、短小芽孢杆菌的筛选
从江苏无锡方鼎鼎养猪场附近的土壤样品称取2g，加入98ml无菌生理盐水，80℃热处理10min后，在37℃摇床上120rpm条件下振荡20min，吸取上清液1ml加入LB培养基中稀释涂布进行菌株分离。通过对菌株进行形态学观察、生理生化实验、16S rDNA分析等手段有效而准确的确定菌种的属种。
最终得到编号为ZS008的菌种，鉴定为短小芽孢杆菌(Bacillus pumilus)。该菌种保藏于中国典型培养物保藏中心，保藏编号为CCTCC No.M2015451，保藏日期为2015年7月13日。
2、凝结芽孢杆菌的筛选
从江苏无锡马山某奶牛养殖场附近的土壤样品称取2g，加入98ml无菌生理盐水，80℃热处理10min后，在37℃摇床上120rpm条件下振荡20min，吸取上清液1ml加入LB培养基中稀释涂布进行菌株分离。通过对菌株进行形态学观察、生理生化实验、16S rDNA分析等手段有效而准确的确定菌种的属种。
最终得到编号为ZS-007的菌种，鉴定为凝结芽孢杆菌(Bacillus coagulans)。
ZS-007于2015年5月3日保藏于中国典型培养物保藏中心，保藏编号为CCTCC M 2015273。
3、复合微生态制剂的制备
复合微生态制剂由短小芽孢杆菌CCTCC No.M2015451、凝结芽孢杆菌CCTCC No.M 2015273、枯草芽孢杆菌CCTCC No.M 2011443、地衣芽孢杆菌CCTCC No.M 2012458复配而成。
其中枯草芽孢杆菌CCTCC No.M 2011443、地衣芽孢杆菌CCTCC No.M 2012458的单菌粉采购自泰州绿圣元生物科技有限公司，其产品规格均为5.0×1010cfu/g。
实施例1～3所配制的微生态制剂产品，各微生物含量如下表1：
表1
Figure PCTCN2016090899-appb-000002
单菌粉按照既定比例混合均匀后，添加无水葡萄糖作为载体，补充到总重量的100％(以芽孢杆菌作为有效成分，加入载体后使产品总重量满足最终活菌数达到4.0×108cfu/g，混合均匀后备用。
加工过程应保证操作环境的清洁，避免污染。控制环境湿度70％以下，各原料水分含量低于13％，该产品制成后常温下保存24个月，-18℃下保存期限可以达到60个月以上，保质期内活菌数稳定。
4、复合益生菌剂在保育仔猪上的应用效果
选择自繁自养同批的杜×长×大三元杂交断奶仔猪90头。依据胎次、体重、公母比例相同或相似的原则，将小猪分为A、B、C 3个组，其中A组为对照组，B试验组1，C组为试验组2，每组设2个重复，每个重复15头，随机分组，分头编号；各组基础日粮相同(正大全价料)，营养水平一致。A组不添加任何酶制剂，B组基础日粮的基础上添加含有短小芽孢杆菌的复合微生态制剂(实施例1)，C组同B组、但不含有短小芽孢杆菌；微生态制剂用量为的用量为1.0g/头·天。仔猪21日龄，平均体重6.5kg断奶，预实验7天，饲养试验30天。饲养地点南京某养殖合作社。
试验结果如表2～4所示。
表2 试验猪只增重和试验期内平均日增重
Figure PCTCN2016090899-appb-000003
表3 饲喂加酶益生素试验各组饲料报酬情况
Figure PCTCN2016090899-appb-000004
表4 饲喂加酶益生素试验各组仔猪腹泻的频率
Figure PCTCN2016090899-appb-000005
仔猪发生拉稀现象后，按每头猪5g的份量使用微生态制剂产品进行治疗。产品用冷开水10mL搅拌后，抽入注射器，并用注射器沿仔猪口角灌服。微生态制剂产品中含有短小芽孢杆菌时，仔猪4-6hr后精神好转，24hr内拉稀终止，恢复食欲。微生态制剂产品中不含有短小芽孢杆菌时，仔猪12hr后精神好转；巩固治疗一次，2天左右拉稀终止，恢复食欲。

Claims (6)

  1. 一株短小芽孢杆菌(Bacillus pumilus)ZS008，保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO.M2015451，保藏日期为2015年7月13日。
  2. 一种保育仔猪用复合微生态芽孢杆菌制剂，其特征在于由以下菌种组合配制成为复合菌剂：短小芽孢杆菌CCTCC NO.M2015451、凝结芽孢杆菌CCTCC NO.M2015273、枯草芽孢杆菌CCTCC NO.M2011443、地衣芽孢杆菌CCTCC NO.M2012458。
  3. 根据权利要求2所述的保育仔猪用复合微生态芽孢杆菌制剂，其特征在于所述复合微生态芽孢杆菌制剂的有效活菌数为1.4×108-7.0×1010cfu/g，各菌种的组成成分如下：
    Figure PCTCN2016090899-appb-100001
  4. 根据权利要求2所述的保育仔猪用复合微生态芽孢杆菌制剂，其特征在于所述凝结芽孢杆菌为凝结芽孢杆菌(Bacillus coagulans)ZS007，保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO:M2015273。
  5. 权利要求2所述的保育仔猪用复合微生态芽孢杆菌制剂的应用，其特征在于提升保育仔猪的生产性能。
  6. 根据权利要求5所述的保育仔猪用复合微生态芽孢杆菌制剂的应用，其特征在于所述复合微生态芽孢杆菌制剂的使用量为1.0～5.0g/头·天。
PCT/CN2016/090899 2015-07-22 2016-07-21 一株短小芽孢杆菌及其保育仔猪用复合微生态制剂 Ceased WO2017012573A1 (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019152791A1 (en) * 2018-02-02 2019-08-08 Novozymes A/S Management of pathogenic lawsonia
CN114272552A (zh) * 2021-12-06 2022-04-05 紫茎泽兰科技(云南)有限公司 一种紫茎泽兰脱毒方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104974967B (zh) * 2015-07-22 2018-04-17 江南大学 一株短小芽孢杆菌及其保育仔猪用复合微生态制剂
TWI569731B (zh) * 2015-12-30 2017-02-11 聯發生物科技股份有限公司 飼料添加菌劑及其製備方法
CN106922951A (zh) * 2015-12-30 2017-07-07 联发生物科技股份有限公司 饲料添加菌剂及其制备方法、菌株筛选方法
CN111548965B (zh) * 2020-05-20 2022-03-18 山东省农业科学院 一株驴源短小芽孢杆菌及其在制备治疗驴驹腹泻的药物上的应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103255080A (zh) * 2013-03-22 2013-08-21 内蒙古和美科盛生物技术有限公司 一株用于乳仔猪养殖的优良凝结芽孢杆菌及其应用
CN104171756A (zh) * 2014-09-02 2014-12-03 双胞胎（集团）股份有限公司 家庭猪场保育猪饲料及其饲喂模式
CN104472862A (zh) * 2014-12-29 2015-04-01 江苏千药堂国医研究院有限公司 一种生物饲料添加剂及其制备方法
CN104974967A (zh) * 2015-07-22 2015-10-14 江南大学 一株短小芽孢杆菌及其保育仔猪用复合微生态制剂
CN105211638A (zh) * 2014-05-26 2016-01-06 瑞普（天津）生物药业有限公司 一种预防和治疗乳仔猪细菌性腹泻的微生态制剂
CN105341451A (zh) * 2015-10-26 2016-02-24 滁州市南谯区施集镇兴牧生态养殖场 一种断奶仔猪专用促消化防腹泻的饲料

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101669576B (zh) * 2008-09-12 2013-02-20 安琪酵母股份有限公司 一种酵母组合物及饲料
RU2014119583A (ru) * 2011-08-24 2015-11-20 ДЮПОНТ НЬЮТРИШЭН БАЙОСАЙНСИЗ ЭйПиЭс Штамм бацилл и содержащая его композиция
CN102505003B (zh) * 2011-12-19 2013-06-26 湖南省微生物研究所 一种畜禽用凝结芽孢杆菌高密度发酵方法及其制备的制剂和应用
CN104263683B (zh) * 2014-09-15 2017-09-29 中国农业科学院饲料研究所 具有益生作用的短小芽孢杆菌315及其应用

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103255080A (zh) * 2013-03-22 2013-08-21 内蒙古和美科盛生物技术有限公司 一株用于乳仔猪养殖的优良凝结芽孢杆菌及其应用
CN105211638A (zh) * 2014-05-26 2016-01-06 瑞普（天津）生物药业有限公司 一种预防和治疗乳仔猪细菌性腹泻的微生态制剂
CN104171756A (zh) * 2014-09-02 2014-12-03 双胞胎（集团）股份有限公司 家庭猪场保育猪饲料及其饲喂模式
CN104472862A (zh) * 2014-12-29 2015-04-01 江苏千药堂国医研究院有限公司 一种生物饲料添加剂及其制备方法
CN104974967A (zh) * 2015-07-22 2015-10-14 江南大学 一株短小芽孢杆菌及其保育仔猪用复合微生态制剂
CN105341451A (zh) * 2015-10-26 2016-02-24 滁州市南谯区施集镇兴牧生态养殖场 一种断奶仔猪专用促消化防腹泻的饲料

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DAI, RONGGUO ET AL.: "To Compare the Effect of Four Kind Bacilli Composites on Performance of Piglets", GANSU ANIMAL AND VETERINARY SCIENCES, vol. 36, no. 1, 31 January 2006 (2006-01-31), pages 14 - 16, ISSN: 1006-799X *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019152791A1 (en) * 2018-02-02 2019-08-08 Novozymes A/S Management of pathogenic lawsonia
US11478514B2 (en) 2018-02-02 2022-10-25 Novozymes A/S Management of pathogenic Lawsonia
US11819525B2 (en) 2018-02-02 2023-11-21 Novozymes A/S Management of pathogenic lawsonia
US12171792B2 (en) 2018-02-02 2024-12-24 Novozymes A/S Bacillus strains for improving feed conversion rates and body weight gain in animals
US12440518B2 (en) 2018-02-02 2025-10-14 Novozymes A/S Bacillus strains for improving feed conversion rates and body weight gain in animals
CN114272552A (zh) * 2021-12-06 2022-04-05 紫茎泽兰科技(云南)有限公司 一种紫茎泽兰脱毒方法

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