WO2016136642A1 - 防腐効果のある医薬の組合せ - Google Patents
防腐効果のある医薬の組合せ Download PDFInfo
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- WO2016136642A1 WO2016136642A1 PCT/JP2016/054976 JP2016054976W WO2016136642A1 WO 2016136642 A1 WO2016136642 A1 WO 2016136642A1 JP 2016054976 W JP2016054976 W JP 2016054976W WO 2016136642 A1 WO2016136642 A1 WO 2016136642A1
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- salt
- pharmaceutical composition
- bacteria
- dorzolamide
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/38—Heterocyclic compounds having sulfur as a ring hetero atom
- A61K31/382—Heterocyclic compounds having sulfur as a ring hetero atom having six-membered rings, e.g. thioxanthenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
- A61K31/5375—1,4-Oxazines, e.g. morpholine
- A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/12—Carboxylic acids; Salts or anhydrides thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the present invention relates to a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof.
- the ophthalmic solution needs to have a certain level of antiseptic effect in order to prevent the growth of fungi and the like with repeated use. Therefore, the above-mentioned Cosopt (registered trademark) ophthalmic solution has benzalkonium as a preservative. Chloride is blended. On the other hand, considering the effect of benzalkonium chloride on patients, Cosopt (registered trademark) ophthalmic solution that does not contain benzalkonium chloride is also sold, but it does not contain preservatives. A single-use unit dose container or a PFMD (Preservative Free Multi Dose) container is used. That is, it has been recognized that an ophthalmic solution containing both dorzolamide and timolol must contain a benzalkonium chloride or ensure the antiseptic effect by the container structure in order to be used repeatedly.
- PFMD Preservative Free Multi Dose
- Patent Document 2 discloses a composition containing dorzolamide and timolol and exhibiting an antiseptic effect without containing benzalkonium chloride. However, in the composition containing timolol, it is clearly described that the antiseptic effect is not exhibited unless a certain amount of boric acid is contained.
- An object of the present invention is to provide a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof, which can sufficiently exhibit an antiseptic effect even when used repeatedly opened and closed. That is.
- the inventors of the present invention may use a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof repeatedly by opening and closing the container even though it does not contain benzalkonium chloride.
- the inventors have found that the antiseptic effect can be sufficiently exhibited, and have completed the present invention.
- the present invention provides the following.
- a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof, which does not contain a preservative or contains a predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- a pharmaceutical composition in a multidose container is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less, The content of boric acid or a salt thereof is less than 0.001% (w / v), A pharmaceutical composition in a multidose container.
- a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof, which does not contain a preservative or contains a predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 1.5 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- a pharmaceutical composition in a reusable container is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 1.5 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof, which does not contain a preservative or contains a predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is an amount such that the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to 1.0 is 1.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- a pharmaceutical composition in a reusable container is an amount such that the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to 1.0 is 1.0 or less, The content of boric acid or a salt thereof is less than 0.001% (w / v), A pharmaceutical composition in a reusable container.
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- a pharmaceutical composition in a reusable container is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less, The content of boric acid or a salt thereof is less than 0.001% (w / v), A pharmaceutical composition in a reusable container.
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 1.5 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- a pharmaceutical composition in a reusable container is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 1.5 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is an amount such that the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to 1.0 is 1.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- a pharmaceutical composition in a reusable container is an amount such that the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to 1.0 is 1.0 or less, The content of boric acid or a salt thereof is less than 0.001% (w / v), A pharmaceutical composition in a reusable container.
- Pharmaceutical composition including edetic acid or a salt thereof, wherein the content of edetic acid or a salt thereof is 0.0001 to 0.1% (w / v).
- a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof, which contains no preservatives other than edetic acid and a salt thereof, or comprises a predetermined amount The predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less,
- the content of the edetic acid or a salt thereof is 0.0001 to 0.1% (w / v),
- the content of boric acid or a salt thereof is less than 0.001% (w / v),
- the salt of edetic acid is monosodium edetate, disodium edetate, tetrasodium edetate or disodium edetate dihydrate, according to any one of (7) to (11) Pharmaceutical composition.
- a product comprising the pharmaceutical composition according to any one of (1) to (28) and a container that can be used repeatedly.
- (30) contains timolol or a salt thereof, does not contain a preservative or contains a predetermined amount
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- (31) contains timolol or a salt thereof, does not contain a preservative or contains a predetermined amount,
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 1.5 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- (32) contains timolol or a salt thereof, does not contain a preservative or contains a predetermined amount,
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is an amount such that the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to 1.0 is 1.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- (33) contains timolol or a salt thereof, does not contain benzalkonium chloride, does not contain preservatives other than benzalkonium chloride, or contains a predetermined amount;
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 2.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- (34) contains timolol or a salt thereof, does not contain benzalkonium chloride, does not contain preservatives other than benzalkonium chloride, or contains a predetermined amount,
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is the amount that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to (A) is 1.5 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- (35) contains timolol or a salt thereof, does not contain benzalkonium chloride, does not contain preservatives other than benzalkonium chloride, or contains a predetermined amount;
- the predetermined amount is inoculated with bacteria so that the concentration of the bacterial solution of Escherichia coli ATCC 8739 is in the range of 10 5 to 10 6 cfu / mL in the test sample comprising the preservative and water. Mix evenly and store the test sample at 20-25 ° C. in the dark. After 7 days, 1 mL of the test sample is collected with a micropipette, and the number of bacteria is measured.
- (A) is an amount such that the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to 1.0 is 1.0 or less,
- the content of boric acid or a salt thereof is less than 0.001% (w / v)
- the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to the number of bacteria at the time of inoculation (A) is 2.0 or less.
- the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to the number of bacteria at the time of inoculation (A) is 1.0 or less.
- the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to the number of bacteria at the time of inoculation (A) is 2.0 or less.
- the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to the number of bacteria at the time of inoculation (A) is 1.0 or less.
- a pharmaceutical composition containing dorzolamide or a salt thereof and timolol or a salt thereof which can sufficiently exhibit an antiseptic effect even when used repeatedly by opening and closing a container. be able to.
- the contained dorzolamide has the chemical name (4S, 6S) -4-Ethylamino-6-methyl-5,6-dihydro-4H-thieno [2,3-b] thiopyran-2- It is a compound represented by sulfonamide 7, 7-dioxide.
- timolol contained is represented by the chemical name (2S) -1-[(1, 1-Dimethylethyl) amino] -3- (4-morpholin-4-yl-1, 2, 5- thiadiazol-3-yloxy) propan-2-ol.
- the contained dorzolamide and timolol may be salts, and are not particularly limited as long as they are pharmaceutically acceptable salts.
- Salts include inorganic acid salts, organic acid salts, quaternary ammonium salts, halogen ion salts, alkali metal salts, alkaline earth metal salts, metal salts, organic amine salts, etc. Can be mentioned.
- the salt with inorganic acid include salts with hydrochloric acid, hydrobromic acid, hydroiodic acid, nitric acid, sulfuric acid, phosphoric acid and the like.
- Salts with organic acids include acetic acid, oxalic acid, fumaric acid, maleic acid, succinic acid, malic acid, citric acid, tartaric acid, adipic acid, gluconic acid, glucoheptic acid, glucuronic acid, terephthalic acid, methanesulfonic acid, alanine , Lactic acid, hippuric acid, 1,2-ethanedisulfonic acid, isethionic acid, lactobionic acid, oleic acid, gallic acid, pamoic acid, polygalacturonic acid, stearic acid, tannic acid, trifluoromethanesulfonic acid, benzenesulfonic acid, p- Examples thereof include salts with toluenesulfonic acid, lauryl sulfate, methyl sulfate, naphthalenesulfonic acid, sulfosalicylic acid and the like.
- Examples of quaternary ammonium salts include salts with methyl bromide, methyl iodide and the like.
- Examples of salts with halogen ions include salts with chloride ions, bromide ions, iodide ions and the like.
- Examples of the salt with an alkali metal include salts with lithium, sodium, potassium and the like.
- Examples of the salt with alkaline earth metal include salts with calcium, magnesium and the like.
- Examples of the metal salt include salts with iron, zinc and the like.
- Salts with organic amines include triethylenediamine, 2-aminoethanol, 2,2-iminobis (ethanol), 1-deoxy-1- (methylamino) -2-D-sorbitol, 2-amino-2- (hydroxy And salts with methyl) -1,3-propanediol, procaine, N, N-bis (phenylmethyl) -1,2-ethanediamine and the like.
- salt of dorzolamide monohydrochloride (dorzolamide hydrochloride) is particularly preferable, and as the salt of timolol, monomaleate (timolol maleate) is particularly preferable.
- the contained dorzolamide, timolol and salts thereof may take the form of hydrate or solvate.
- the content of dorzolamide or a salt thereof is not particularly limited as long as it is an amount sufficient to achieve desired drug efficacy and antiseptic effect, but is 0.1 to 5% (w / v) is preferred, 0.2 to 3% (w / v) is more preferred, 0.5 to 2% (w / v) is more preferred, and 0.7 to 1.2% (w / v) is further preferred. More preferred is 1% (w / v). Most preferred is 1% (w / v) or 2% (w / v).
- these values are content converted into the free dorzolamide.
- “% (w / v)” means the mass (g) of the target component (here, Dorzolamide) contained in 100 mL of the pharmaceutical composition of the present invention. The same applies hereinafter unless otherwise specified.
- the content of timolol or a salt thereof is not particularly limited as long as it is an amount sufficient to exhibit a desired medicinal effect, but is 0.01 to 2% (w / v).
- 0.05 to 1% (w / v) is more preferable, 0.1 to 0.8% (w / v) is more preferable, and 0.2 to 0.7% (w / v) is even more preferable 0.5% (w / v) is particularly preferable.
- these values are contents converted to free timolol.
- the content of dorzolamide or a salt thereof is preferably 0.1 to 10 times, more preferably 0.5 to 8 times, and more preferably 1 to 3 times the content of timolol or a salt thereof from the viewpoint of therapeutic effect and antiseptic effect. More preferably, it is 5 times.
- dorzolamide which is an active ingredient of the medicine, or a salt thereof itself exerts an antiseptic effect, so that it does not contain a preservative or can be contained in a predetermined amount.
- the “predetermined amount” refers to, for example, an amount that does not exhibit a preservative effect alone.
- Escherichia coli ATCC 8739 Inoculate the bacteria so that the concentration of the bacterial solution is in the range of 10 5 to 10 6 cfu / mL, mix evenly, and after 7 days have passed since the test sample was stored at 20-25 ° C. in the dark.
- the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to the number of bacteria (A) when the number of viable bacteria is measured is 2.0 or less.
- the amount is preferably 1.5 or less, more preferably 1.2 or less, still more preferably 1.0 or less, particularly preferably 0.8 or less, and most preferably 0.7 or less.
- benzalkonium chloride when benzalkonium chloride is contained, it is preferable that benzalkonium chloride is contained in a predetermined amount.
- the “predetermined amount” refers to, for example, an amount that does not exhibit an antiseptic effect alone.
- the ATCC of Escherichia coli is used. Bacteria were inoculated so that the concentration of the bacterial solution of 8739 was in the range of 10 5 to 10 6 cfu / mL, mixed uniformly, and after 7 days had passed since the test sample was stored at 20-25 ° C. in the dark.
- the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B / A) to the number of bacteria (A) when 1 mL of a test sample was collected with a micropipette and the number of viable bacteria was measured was 2.0 or less
- the amount may be 1.5 or less, more preferably 1.2 or less, still more preferably 1.0 or less, particularly preferably 0.8 or less, and most preferably 0.7 or less.
- benzalkonium chloride is preferably 0.001% (w / v) or less, more preferably 0.0007% (w / v) or less, and 0.0005% (w / v) or less.
- quaternary ammonium salt used as a preservative other than benzalkonium chloride has a small content or is not contained.
- examples of quaternary ammonium salts other than benzalkonium chloride include benzalkonium bromide, benzethonium chloride, benzyldodecinium bromide and the like.
- the quaternary ammonium salt when a quaternary ammonium salt other than benzalkonium chloride is contained, the quaternary ammonium salt is preferably contained in a predetermined amount.
- the “predetermined amount” refers to, for example, an amount that does not exhibit a preservative effect, and specifically, in a test sample comprising the preservative (a quaternary ammonium salt other than benzalkonium chloride) and water.
- a quaternary ammonium salt other than benzalkonium chloride is preferably not contained in an amount of 0.01% (w / v) or more (content is less than 0.01% (w / v)), More preferably 0.005% (w / v) or more is not included, 0.001% (w / v) or more is more preferably included, and 0.0005% (w / v) or more is not included. Is more preferable, it is particularly preferable that 0.0001% (w / v) or more is not included, and most preferable that it is not substantially included.
- a preservative other than the quaternary ammonium salt is contained in a small amount or not contained.
- preservatives other than the quaternary ammonium salt include sorbic acid, potassium sorbate, methyl paraoxybenzoate, propyl paraoxybenzoate, chlorobutanol, cyclohexidine, boric acid or a salt thereof, edetic acid or a salt thereof.
- the preservative when a preservative other than the quaternary ammonium salt is contained, it is preferable that the preservative is contained in a predetermined amount.
- the “predetermined amount” refers to, for example, an amount exhibiting an antiseptic effect alone.
- Escherichia coli ATCC 8739 Inoculate the bacteria so that the concentration of the bacterial solution is in the range of 10 5 to 10 6 cfu / mL, mix evenly, and after 7 days have passed since the test sample was stored at 20-25 ° C. in the dark.
- a common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B / A) to the number of bacteria (A) obtained by collecting 1 mL of the sample with a micropipette and measuring the number of viable bacteria is 2.0 or less, preferably May be an amount that is 1.5 or less, more preferably 1.2 or less, even more preferably 1.0 or less, particularly preferably 0.8 or less, and most preferably 0.7 or less.
- 0.5% (w / v) or more of preservatives other than quaternary ammonium salts are not contained (content is 0.5% (w Less than 0.10% (w / v) (more than 0.10% (w / v) content), more preferably 0.05% (w / v) ) Not more than 0.01% (w / v), more preferably not more than 0.005% (w / v), more preferably 0.001% It is particularly preferable that (w / v) or more is not included, and it is most preferable that it is not substantially included.
- the salt of boric acid include borax, sodium borate, potassium borate and the like.
- boric acid salt when contained in the pharmaceutical composition of the present invention, these values are contents converted to free boric acid.
- edetic acid or a salt thereof is often added to a pharmaceutical composition as a stabilizer, but these are also known to have antiseptic effects, and edetic acid or a salt thereof is added to the pharmaceutical composition of the present invention.
- the total content is more than 0% (w / v) (0.0001% or more, 0.0005% or more, 0.001% or more, 0.002% or more, 0.003% or more, 0 0.005% or more, 0.007% or more) is preferably 0.5% (w / v) or less, more preferably 0.3% (w / v) or less, and 0.1% (w / v) or less. More preferably, 0.08% (w / v) or less is more preferable, 0.05% (w / v) or less is more preferable, 0.03% (w / v) or less is particularly preferable, and 0.01% ( Most preferred is w / v) or less.
- salts of edetic acid include monosodium edetate, disodium edetate, tetrasodium edetate, sodium citrate and the like, disodium edetate is preferred, and disodium edetate is particularly preferred.
- the pharmaceutical composition of the present invention preferably contains no preservatives other than edetic acid and its salts.
- the pharmaceutical composition of this invention contains antiseptics other than edetic acid and its salt, it is preferable that the preservative is contained in predetermined amount.
- the “predetermined amount” refers to, for example, an amount that does not exhibit a sufficient antiseptic effect.
- Escherichia coli Escherichia coli
- the concentration of the ATCC 8739 bacterial solution is in the range of 10 5 to 10 6 cfu / mL and mixing it uniformly, and storing the test sample at 20 to 25 ° C. in the dark.
- 1 mL of a test sample was collected with a micropipette, and the common logarithm of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to the number of bacteria (A) when the number of viable bacteria was measured was 2.0.
- the amount may be 1.5 or less, more preferably 1.2 or less, still more preferably 1.0 or less, particularly preferably 0.8 or less, and most preferably 0.7 or less.
- the pharmaceutical composition of this invention contains antiseptics other than edetic acid and its salt, and benzalkonium chloride, it is preferable that the preservative is contained in predetermined amount.
- the “predetermined amount” refers to, for example, an amount that does not exhibit a sufficient antiseptic effect. Specifically, in a test sample composed of the preservative and water, the ATCC of Escherichia coli is used.
- Bacteria were inoculated so that the concentration of the bacterial solution of 8739 was in the range of 10 5 to 10 6 cfu / mL, mixed uniformly, and after 7 days had passed since the test sample was stored at 20-25 ° C. in the dark.
- the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B / A) to the number of bacteria (A) when 1 mL of a test sample was collected with a micropipette and the number of viable bacteria was measured was 2.0 or less
- the amount may be 1.5 or less, more preferably 1.2 or less, still more preferably 1.0 or less, particularly preferably 0.8 or less, and most preferably 0.7 or less.
- the salt of edetic acid or its hydrate is contained in the pharmaceutical composition of this invention, these values are content calculated based on the mass of the salt of edetic acid or its hydrate.
- the preservative in the present invention refers to a component represented as a preservative in a pharmaceutical composition, and a component that exhibits a preservative effect but is not represented as a preservative, such as dorzolamide or a salt thereof in the pharmaceutical composition of the present invention. Does not include.
- additives may be used as necessary.
- the additives include surfactants, buffering agents, tonicity agents, stabilizers, antioxidants, high molecular weight weights. Coalescence etc. can be added.
- a surfactant that can be used as a pharmaceutical additive for example, a cationic surfactant, an anionic surfactant, or a nonionic surfactant can be blended.
- anionic surfactants include phospholipids, and examples of phospholipids include lecithin.
- Examples of cationic surfactants include alkylamine salts, alkylamine polyoxyethylene adducts, fatty acid triethanolamine monoester salts, acylaminoethyl diethylamine salts, fatty acid polyamine condensates, alkylimidazolines, 1-acylaminoethyl- Examples thereof include 2-alkyl imidazoline, 1-hydroxylethyl-2-alkyl imidazoline and the like.
- Examples of nonionic surfactants include polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene hydrogenated castor oil, polyoxyethylene castor oil, polyoxyethylene polyoxypropylene glycol, sucrose fatty acid esters, Vitamin E TPGS etc. are mentioned.
- polyoxyethylene fatty acid ester examples include polyoxyl 40 stearate.
- polyoxyethylene sorbitan fatty acid ester examples include polysorbate 80, polysorbate 60, polysorbate 40, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan trioleate, polysorbate 65, and the like.
- polyoxyethylene hydrogenated castor oil various polyoxyethylene hydrogenated castor oils having different polymerization numbers of ethylene oxide can be used, and the polymerization number of ethylene oxide is preferably 10 to 100, more preferably 20 to 80, ⁇ 70 are particularly preferred and 60 is most preferred.
- Specific examples of the polyoxyethylene hydrogenated castor oil include polyoxyethylene hydrogenated castor oil 10, polyoxyethylene hydrogenated castor oil 40, polyoxyethylene hydrogenated castor oil 50, polyoxyethylene hydrogenated castor oil 60, and the like.
- polyoxyethylene castor oil various polyoxyethylene castor oils having different polymerization numbers of ethylene oxide can be used, and the polymerization number of ethylene oxide is preferably 5 to 100, more preferably 20 to 50, and more preferably 30 to 40 Is particularly preferred and 35 is most preferred.
- polyoxyethylene castor oil include polyoxyl 5 castor oil, polyoxyl 9 castor oil, polyoxyl 15 castor oil, polyoxyl 35 castor oil, polyoxyl 40 castor oil, and the like.
- polyoxyethylene polyoxypropylene glycol polyoxyethylene (160) polyoxypropylene (30) glycol, polyoxyethylene (42) polyoxypropylene (67) glycol, polyoxyethylene (54) polyoxypropylene (39) And glycol, polyoxyethylene (196) polyoxypropylene (67) glycol, polyoxyethylene (20) polyoxypropylene (20) glycol and the like.
- sucrose fatty acid ester examples include sucrose stearate.
- Vitamin E TPGS is also called tocopherol polyethylene glycol 1000 succinate.
- the content of the surfactant when the surfactant is added to the pharmaceutical composition in which the preservative of the present invention is used can be appropriately adjusted depending on the type of the surfactant, etc., but is 0.001 to 10%.
- (W / v) is preferable, 0.01 to 5% (w / v) is more preferable, 0.1 to 3% (w / v) is further preferable, and 0.2 to 2% (w / v) is preferable. Most preferred.
- the pharmaceutical composition of the present invention may contain a buffering agent that can be used as a pharmaceutical additive.
- the buffer include phosphoric acid or a salt thereof, citric acid or a salt thereof, acetic acid or a salt thereof, carbonic acid or a salt thereof, tartaric acid or a salt thereof, ⁇ -aminocaproic acid, trometamol, and the like.
- the phosphate include sodium phosphate, sodium dihydrogen phosphate, disodium hydrogen phosphate, potassium phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, and the citrate includes citric acid. Sodium, disodium citrate and the like can be mentioned.
- the acetate include sodium acetate and potassium acetate.
- Examples of the carbonate include sodium carbonate and sodium bicarbonate.
- Examples of the tartrate include sodium tartrate, Examples include potassium tartrate.
- Citric acid or a salt thereof is preferred, and sodium citrate is particularly preferred.
- the content of the buffer when blending the buffer with the pharmaceutical composition of the present invention can be appropriately adjusted depending on the type of the buffer, etc., but is preferably 0.001 to 10% (w / v), 0 0.01 to 5% (w / v) is more preferable, 0.1 to 3% (w / v) is more preferable, and 0.2 to 2% (w / v) is most preferable.
- an isotonic agent that can be used as a pharmaceutical additive can be appropriately blended.
- isotonic agents include ionic and nonionic tonicity agents.
- the ionic tonicity agent include sodium chloride, potassium chloride, calcium chloride, magnesium chloride, and sodium chloride is preferable.
- Nonionic tonicity agents include glycerin, propylene glycol, sorbitol, mannitol and the like, with mannitol being preferred.
- the content of the tonicity agent when blended with the isotonic agent in the pharmaceutical composition of the present invention can be appropriately adjusted depending on the type of tonicity agent and the like, but is 0.01 to 10% (w / v) is preferred, 0.02 to 7% (w / v) is more preferred, 0.1 to 5% (w / v) is more preferred, 0.5 to 4% (w / v) is particularly preferred, Most preferred is 0.8 to 3% (w / v).
- a stabilizer that can be used as a pharmaceutical additive can be appropriately blended.
- stabilizers include edetic acid, monosodium edetate, disodium edetate, tetrasodium edetate, sodium citrate and the like, disodium edetate is preferred, and disodium edetate dihydrate is Particularly preferred.
- the content of the stabilizer when the stabilizer is added to the pharmaceutical composition of the present invention can be appropriately adjusted depending on the kind of the stabilizer, etc., but is 0.0001 to 0.5% (w / v ), Preferably 0.0005 to 0.3% (w / v), more preferably 0.001 to 0.1% (w / v), and 0.002 to 0.08% (w / v). ) Is more preferred, 0.003 to 0.05% (w / v) is more preferred, 0.005 to 0.03% (w / v) is particularly preferred, and 0.007 to 0.01% (w / v) v) is most preferred.
- an antioxidant that can be used as a pharmaceutical additive can be appropriately blended.
- antioxidants include ascorbic acid, tocopherol, dibutylhydroxytoluene, butylhydroxyanisole, sodium erythorbate, propyl gallate, sodium sulfite and the like.
- the content of the antioxidant when the antioxidant is mixed with the pharmaceutical composition of the present invention can be appropriately adjusted depending on the kind of the antioxidant, etc., but is 0.0001 to 1% (w / v).
- 0.0005 to 0.1% (w / v) is more preferable
- 0.001 to 0.02% (w / v) is more preferable
- 0.005 to 0.010% (w / v) is more preferable.
- a high molecular weight polymer that can be used as a pharmaceutical additive can be appropriately blended.
- high molecular weight polymers include methylcellulose, ethylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hydroxyethylmethylcellulose, hydroxypropylmethylcellulose, carboxymethylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose acetate succinate, hydroxypropylmethylcellulose phthalate, Examples thereof include carboxymethyl ethyl cellulose, cellulose acetate phthalate, polyvinyl pyrrolidone, polyvinyl alcohol, carboxy vinyl polymer, polyethylene glycol and the like, and hydroxyethyl cellulose is preferred.
- the content of the high molecular weight polymer in the case where the high molecular weight polymer is blended with the pharmaceutical composition of the present invention can be appropriately adjusted depending on the type of the high molecular weight polymer, etc., but is 0.001 to 5% (w / v) is preferred, 0.01 to 3% (w / v) is more preferred, 0.1 to 2% (w / v) is more preferred, and 0.2 to 1% (w / v) is most preferred.
- a pH adjuster that can be used as a pharmaceutical additive can be appropriately blended.
- the pH adjusting agent include hydrochloric acid, phosphoric acid, citric acid, acetic acid, sodium hydroxide, potassium hydroxide, sodium carbonate, sodium hydrogen carbonate and the like.
- the pharmaceutical composition of the present invention particularly preferably contains hydroxyethyl cellulose as a high molecular weight polymer, mannitol as an isotonic agent, and citric acid or a salt thereof as a buffer in combination.
- the pharmaceutical composition of the present invention can be rapidly sterilized.
- the content of each component is 0.001 to 5% (w / v) for hydroxyethyl cellulose and 0.01 to 10% for mannitol.
- citric acid or a salt thereof is preferably 0.001 to 10% (w / v), hydroxyethyl cellulose is 0.01 to 3% (w / v), mannitol is 0.02 to 7% (w / v), citric acid or a salt thereof is more preferably 0.01 to 5% (w / v), hydroxyethyl cellulose is 0.1 to 2% (w / v), and mannitol is 0.
- citric acid or a salt thereof is 0.1 to 3% (w / v)
- hydroxyethyl cellulose is 0.2 to 1% (w / v)
- Mannitol is 0.8-3% ( / V)
- citric acid or its salt is 0.2 ⁇ 2% (w / v).
- the pH of the pharmaceutical composition of the present invention is preferably 4.0 to 8.0, more preferably 5.0 to 7.5, still more preferably 5.5 to 7.3, and 5.5 to 7.0. More preferred is 5.5 to 6.8, and most preferred is 6.0 to 6.8.
- the pharmaceutical composition of the present invention is placed in a container that can be used repeatedly.
- the container that can be used repeatedly include a multi-dose type container and a recapping unit dose container.
- a multi-dose type container is a container that can be freely opened and closed for the purpose of using it multiple times.
- a PFMD (Preservative Free Multi Dose) container having a special structure for exhibiting an antiseptic effect such as a backflow prevention function is not included.
- the recapping unit dose container is a unit dose container that can be used repeatedly by recapping.
- limiting in particular in the raw material of a container For example, containers made from polyethylene (PE), a product made from polypropylene (PP), a product made from polyethylene terephthalate (PET), etc. can be used.
- the dosage form of the pharmaceutical composition of the present invention is not particularly limited as long as it can be used as a pharmaceutical product, but an eye drop is particularly preferable and can be produced according to a usual method in the technical field.
- the pharmaceutical composition of the present invention is useful as a therapeutic agent for glaucoma or ocular hypertension.
- the dosage is not particularly limited as long as it is sufficient to achieve a desired drug effect, but it is preferable to instill 1 to 3 drops at a time, 1 to 3 times a day, It is more preferable to apply 1 to 2 drops once or 1 to 2 times a day, and it is most preferable to apply 1 drop once or twice a day.
- the pharmaceutical composition of the present invention is useful for contact lenses (wearers).
- the type of contact lens to be applied is not particularly limited, and specific examples include hard contact lenses, soft contact lenses, and the like, and oxygen permeable contact lenses may be used.
- Examples of the soft contact lens include a hydrous soft contact lens, a non-hydrous soft contact lens, and a (nonionic) silicone hydrogel soft contact lens.
- composition of the present invention also applies to a product comprising the pharmaceutical composition of the present invention and a container (contains the pharmaceutical composition) that can be used repeatedly, and a method for improving antiseptic efficacy. Is done.
- the method for improving the preservative effect of the present invention contains timolol or a salt thereof, does not contain benzalkonium chloride, does not contain boric acid or a salt thereof, or has a boric acid or a salt content of 0.001%. It is preferable that the preservative efficacy is improved by further containing dorzolamide or a salt thereof in a pharmaceutical composition that is less than (w / v) and is contained in a container that can be used repeatedly.
- a pharmaceutical composition before containing dorzolamide or a salt thereof is prepared by adding a bacterial solution concentration of ATCC 8739 of Escherichia coli to 10 5 to 10 6. Inoculate the bacteria so that it is within the range of cfu / mL, mix uniformly, and store the pharmaceutical composition at 20-25 ° C. under light shielding, and after 7 days, 1 mL of the pharmaceutical composition is micropipetted. It is preferable that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B / A) to the number of bacteria (A) when collected and the number of viable bacteria is measured is 2.0 or less. Or less, more preferably 1.0 or less.
- the pharmaceutical composition after the above-mentioned dorzolamide or a salt thereof is contained the bacterial solution concentration of ATCC 8739 of Escherichia coli is 10 5.
- the pharmaceutical composition was stored at 20-25 ° C. under light shielding, and after 7 days, the pharmaceutical composition was micropipetted. It is preferable that the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B / A) to the number of bacteria (A) when measuring the number of viable bacteria (B / A) is 2.5 or more.
- the pharmaceutical composition after containing the above-mentioned dorzolamide or a salt thereof is the standard “Category IA” according to the 16th revised Japanese Pharmacopoeia reference information “Preservation efficacy test method”. "Is preferably satisfied.
- Formulation Example A typical formulation example of the present invention is shown below.
- the amount of each component is the content in 1 mL of the formulation.
- a desired composition can be obtained by appropriately adjusting the types and blending amounts of dorzolamide, timolol and additives in Preparation Examples 1 and 2.
- Antiseptic effect test (1) 1. Preparation of test preparation Liquid A obtained by dissolving dorzolamide hydrochloride (22.26 mg), timolol maleate (6.83 mg), sodium citrate (2.94 mg), mannitol (16 mg) in water and sterilizing by filtration; Separately, Hydroxyethylcellulose (4.75 mg) was dissolved in water and mixed with Liquid B, which had been subjected to high-pressure steam sterilization. After adjusting the pH to 5.7 with a pH regulator, water was added to make the total volume 1 mL. The formulation of Example 1 was prepared.
- Example 1 (in 1 mL) Dorzolamide hydrochloride 22.26mg Timolol maleate 6.83mg Sodium citrate hydrate 2.94mg Mannitol 16mg Hydroxyethylcellulose 4.75mg Dilute hydrochloric acid appropriate amount Sodium hydroxide appropriate amount Purified water appropriate amount pH 5.7
- Example 2 Preparations of Example 2 and Comparative Examples 1 and 2 were prepared in the same manner as the preparation method of Example 1.
- Example 2 (in 1 mL) Dorzolamide hydrochloride 11.13mg Timolol maleate 6.83mg Sodium citrate hydrate 2.94mg Mannitol 30mg Hydroxyethylcellulose 4.75mg Dilute hydrochloric acid appropriate amount Sodium hydroxide appropriate amount Purified water appropriate amount pH 5.7
- Bacteria E. coli, Escherichia Coli ATCC 8739 (also called E.coli) Pseudomonas aeruginosa ATCC 9027 (also called P. aeruginosa) Staphylococcus aureus ATCC 6538 (also called S. aureus)
- yeasts and molds Candida, Candida albicans ATCC 10231 (also called C. albicans) Aspergillus brasiliensis ATCC16404 (also called A. brasiliensis)
- the inoculated bacterial solution was inoculated into the test sample so that the concentration of the bacterial solution in the test sample consisting of each preparation was 10 5 to 10 6 cells / mL (for all 5 species).
- an inoculum solution was prepared so as to be 10 7 to 10 8 cfu / mL, and Examples 1-2 and Comparative Examples were prepared so that this inoculum solution would be 10 5 to 10 6 cfu / mL.
- Each inoculum was inoculated into a test sample consisting of 1-2 preparations and mixed uniformly to prepare a sample. These samples were stored at 20 to 25 ° C.
- Test results and discussion Table 1 shows the test results.
- the test results in Table 1 show the common logarithm of the ratio (B / A) of the number of bacteria at the time of inoculation (B) to the number of bacteria (A) when the number of viable bacteria is measured. In this case, the number of viable bacteria at the time of inspection is reduced to 10% of the number of inoculated bacteria.
- Example 3 (in 1 mL) Dorzolamide hydrochloride 11.13mg Timolol maleate 6.83mg Sodium chloride 9mg Dilute hydrochloric acid Appropriate amount Sodium hydroxide Appropriate amount Purified water Appropriate amount pH 5.65
- Example 4 (in 1 mL) Dorzolamide hydrochloride 11.13mg Timolol maleate 6.83mg Sodium chloride 9mg Dilute hydrochloric acid Appropriate amount Sodium hydroxide Appropriate amount Purified water Appropriate amount pH 6.5
- Example 5 (in 1 mL) Dorzolamide hydrochloride 11.13mg Timolol maleate 6.83mg Sodium citrate hydrate 2.94mg Mannitol 30mg Hydroxyethylcellulose 4.75mg Dilute hydrochloric acid Appropriate amount Sodium hydroxide Appropriate amount Purified water Appropriate amount pH 5.65
- Example 6 (in 1 mL) Dorzolamide hydrochloride 11.13mg Timolol maleate 6.83mg Sodium citrate hydrate 2.94mg Mannitol 30mg Hydroxyethylcellulose 4.75mg Edetate disodium dihydrate 0.5mg Dilute hydrochloric acid Appropriate amount Sodium hydroxide Appropriate amount Purified water Appropriate amount pH 5.65
- Test method Preservation efficacy test (1) A preservative efficacy test was performed in the same manner as the test method. However, the sampling points were 7 days, 14 days or 28 days later.
- Test results and discussion Table 2 shows the test results.
- the test results in Table 2 are shown as common logarithms of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to the number of bacteria (A) when the number of viable bacteria was measured.
- Test method 2 Preservation efficacy test (1) A preservative efficacy test was performed in the same manner as the test method. However, the sampling point was 7 days later.
- Test results and discussion Table 3 shows the test results.
- the test results in Table 3 are shown as common logarithms of the ratio (B / A) of the number of bacteria (B) at the time of inoculation to the number of bacteria (A) when the number of viable bacteria was measured.
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Abstract
Description
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
マルチドーズ型容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.5以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.5以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記エデト酸又はその塩の含有量が、0.0001~0.1%(w/v)であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
前記エデト酸又はその塩の含有量が、0.0001~0.1%(w/v)であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.5以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.5以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下である、方法。
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.5以下である、方法。
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.0以下である、方法。
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下である、方法。
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.5以下である、方法。
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が1.0以下である、方法。
本発明の医薬組成物において、含有されるドルゾラミドは、化学名(4S, 6S)-4-Ethylamino-6-methyl-5, 6-dihydro-4H-thieno[2, 3-b]thiopyran-2-sulfonamide 7, 7-dioxideで表される化合物である。
無機酸との塩としては、塩酸、臭化水素酸、ヨウ化水素酸、硝酸、硫酸、リン酸等との塩が挙げられる。
有機酸との塩としては、酢酸、シュウ酸、フマル酸、マレイン酸、コハク酸、リンゴ酸、クエン酸、酒石酸、アジピン酸、グルコン酸、グルコヘプト酸、グルクロン酸、テレフタル酸、メタンスルホン酸、アラニン、乳酸、馬尿酸、1,2-エタンジスルホン酸、イセチオン酸、ラクトビオン酸、オレイン酸、没食子酸、パモ酸、ポリガラクツロン酸、ステアリン酸、タンニン酸、トリフルオロメタンスルホン酸、ベンゼンスルホン酸、p-トルエンスルホン酸、硫酸ラウリル、硫酸メチル、ナフタレンスルホン酸、スルホサリチル酸等との塩が挙げられる。
四級アンモニウム塩としては、臭化メチル、ヨウ化メチル等との塩が挙げられる。
ハロゲンイオンとの塩としては、塩化物イオン、臭化物イオン、ヨウ化物イオン等との塩が挙げられる。
アルカリ金属との塩としては、リチウム、ナトリウム、カリウム等との塩が挙げられる。
アルカリ土類金属との塩としては、カルシウム、マグネシウム等との塩が挙げられる。
金属塩としては、鉄、亜鉛等との塩が挙げられる。
有機アミンとの塩としては、トリエチレンジアミン、2-アミノエタノール、2,2-イミノビス(エタノール)、1-デオキシ-1-(メチルアミノ)-2-D-ソルビトール、2-アミノ-2-(ヒドロキシメチル)-1,3-プロパンジオール、プロカイン、N,N-ビス(フェニルメチル)-1,2-エタンジアミン等との塩が挙げられる。
ドルゾラミドの塩としては、一塩酸塩(ドルゾラミド塩酸塩)が特に好ましく、チモロールの塩としては、一マレイン酸塩(チモロールマレイン酸塩)が特に好ましい。
アニオン性界面活性剤の例としては、リン脂質等が挙げられ、リン脂質としてはレシチン等が挙げられる。
カチオン性界面活性剤の例としては、アルキルアミン塩、アルキルアミンポリオキシエチレン付加物、脂肪酸トリエタノールアミンモノエステル塩、アシルアミノエチルジエチルアミン塩、脂肪酸ポリアミン縮合物、アルキルイミダゾリン、1-アシルアミノエチル-2-アルキルイミダゾリン、1-ヒドロキシルエチル‐2-アルキルイミダゾリン等が挙げられる。
非イオン性界面活性剤の例としては、ポリオキシエチレン脂肪酸エステル、ポリオキシエチレンソルビタン脂肪酸エステル、ポリオキシエチレン硬化ヒマシ油、ポリオキシエチレンヒマシ油、ポリオキシエチレンポリオキシプロピレングリコール、ショ糖脂肪酸エステル、ビタミンE TPGS等が挙げられる。
リン酸塩としては、リン酸ナトリウム、リン酸二水素ナトリウム、リン酸水素二ナトリウム、リン酸カリウム、リン酸二水素カリウム、リン酸水素二カリウム等が挙げられ、クエン酸塩としては、クエン酸ナトリウム、クエン酸二ナトリウム等が挙げられ、酢酸塩としては、酢酸ナトリウム、酢酸カリウム等が挙げられ、炭酸塩としては、炭酸ナトリウム、炭酸水素ナトリウム等が挙げられ、酒石酸塩としては、酒石酸ナトリウム、酒石酸カリウム等が挙げられる。クエン酸又はその塩が好ましく、クエン酸ナトリウムが特に好ましい。
本発明の医薬組成物に緩衝剤を配合する場合の緩衝剤の含有量は、緩衝剤の種類などにより適宜調整することができるが、0.001~10%(w/v)が好ましく、0.01~5%(w/v)がより好ましく、0.1~3%(w/v)がさらに好ましく、0.2~2%(w/v)が最も好ましい。
以下に本発明の代表的な製剤例を示す。なお、下記製剤例において各成分の配合量は製剤1mL中の含量である。
ドルゾラミド 10mg
チモロール 5mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
ドルゾラミド 20mg
チモロール 5mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
1.被験製剤の調製
ドルゾラミド塩酸塩(22.26mg)、チモロールマレイン酸塩(6.83mg)、クエン酸ナトリウム(2.94mg)、マンニトール(16mg)を水に溶解し濾過滅菌を行った液Aと、別途、ヒドロキシエチルセルロース(4.75mg)を水に溶解して高圧蒸気滅菌を行った液Bを混合し、pH調節剤にてpH5.7とした後、水を加えて全量を1mLとすることにより、実施例1の製剤を調製した。
ドルゾラミド塩酸塩 22.26mg
チモロールマレイン酸塩 6.83mg
クエン酸ナトリウム水和物 2.94mg
マンニトール 16mg
ヒドロキシエチルセルロース 4.75mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.7
ドルゾラミド塩酸塩 11.13mg
チモロールマレイン酸塩 6.83mg
クエン酸ナトリウム水和物 2.94mg
マンニトール 30mg
ヒドロキシエチルセルロース 4.75mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.7
チモロールマレイン酸塩 6.83mg
クエン酸ナトリウム水和物 2.94mg
マンニトール 30mg
ヒドロキシエチルセルロース 4.75mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.8
ドルゾラミド塩酸塩 11.13mg
クエン酸ナトリウム水和物 2.94mg
マンニトール 30mg
ヒドロキシエチルセルロース 4.75mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.7
接種菌として以下の菌株を使用した。
細菌:
大腸菌,Escherichia Coli ATCC 8739(E.coliともいう)
緑膿菌,Pseudomonas aeruginosa ATCC 9027(P.aeruginosaともいう)
黄色ブドウ球菌,Staphylococcus aureus ATCC 6538(S.aureusともいう)
酵母菌およびカビ類:
カンジダ,Candida albicans ATCC 10231(C.albicansともいう)
クロコウジカビ,Aspergillus brasiliensis ATCC16404(A.brasiliensisともいう)
試験結果を表1に示す。表1の試験結果は、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値で示しており、例えば「1」の場合には、検査時の生菌数が接種菌数の10%に減少したことを示している。
防腐効力試験(2)
1.被験製剤の調製
実施例1の調製方法と同様の方法にて、実施例3~6の製剤を調製した。
ドルゾラミド塩酸塩 11.13mg
チモロールマレイン酸塩 6.83mg
塩化ナトリウム 9mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.65
ドルゾラミド塩酸塩 11.13mg
チモロールマレイン酸塩 6.83mg
塩化ナトリウム 9mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 6.5
ドルゾラミド塩酸塩 11.13mg
チモロールマレイン酸塩 6.83mg
クエン酸ナトリウム水和物 2.94mg
マンニトール 30mg
ヒドロキシエチルセルロース 4.75mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.65
ドルゾラミド塩酸塩 11.13mg
チモロールマレイン酸塩 6.83mg
クエン酸ナトリウム水和物 2.94mg
マンニトール 30mg
ヒドロキシエチルセルロース 4.75mg
エデト酸二ナトリウム二水和物 0.5mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.65
防腐効力試験(1)の2.試験方法と同様の方法にて防腐効力試験を実施した。ただし、サンプリングポイントは、7日後、14日後又は28日後とした。
試験結果を表2に示す。表2の試験結果は、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値で示している。
防腐効力試験(3)
1.被験製剤の調製
実施例1の調製方法と同様の方法にて、比較例3及び4の製剤を調製した。
チモロールマレイン酸塩 6.83mg
クエン酸ナトリウム水和物 2.94mg
ヒドロキシエチルセルロース 4.75mg
エデト酸二ナトリウム二水和物 0.5mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.65
クエン酸ナトリウム水和物 2.94mg
ヒドロキシエチルセルロース 4.75mg
エデト酸二ナトリウム二水和物 0.5mg
希塩酸 適量
水酸化ナトリウム 適量
精製水 適量
pH 5.65
防腐効力試験(1)の2.試験方法と同様の方法にて防腐効力試験を実施した。ただし、サンプリングポイントは、7日後とした。
試験結果を表3に示す。表3の試験結果は、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値で示している。
Claims (18)
- ドルゾラミド又はその塩及びチモロール又はその塩を含有する医薬組成物であって、防腐剤を含まない又は所定量で含み、
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。 - エデト酸又はその塩を含み、エデト酸又はその塩の含有量が0.0001~0.1%(w/v)である、請求項1に記載の医薬組成物。
- ドルゾラミド又はその塩及びチモロール又はその塩を含有する医薬組成物であって、エデト酸及びその塩以外の防腐剤を含まず、
前記エデト酸又はその塩の含有量が、0.0001~0.1%(w/v)であり、
繰り返し使用可能な容器に入れられた、医薬組成物。 - ドルゾラミド又はその塩及びチモロール又はその塩を含有する医薬組成物であって、エデト酸及びその塩以外の防腐剤を含まない又は所定量で含み、
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
前記エデト酸又はその塩の含有量が、0.0001~0.1%(w/v)であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた、医薬組成物。 - エデト酸の塩が、エデト酸一ナトリウム、エデト酸二ナトリウム、エデト酸四ナトリウム又はエデト酸二ナトリウム二水和物である、請求項2~4のいずれかに一項に記載の医薬組成物。
- ドルゾラミド又はその塩がドルゾラミド塩酸塩である、請求項1~5のいずれかに一項に記載の医薬組成物。
- チモロール又はその塩がチモロールマレイン酸塩である、請求項1~6のいずれか一項に記載の医薬組成物。
- ドルゾラミド又はその塩の含有量が0.1-5%(w/v)である、請求項1~7のいずれか一項に記載の医薬組成物。
- チモロール又はその塩の含有量が0.01-2%(w/v)である、請求項1~8のいずれか一項に記載の医薬組成物。
- ホウ酸又はその塩を含まない、請求項1~9のいずれか一項に記載の医薬組成物。
- さらに高分子量重合体を含有する、請求項1~10のいずれか一項に記載の医薬組成物。
- さらに非イオン性等張化剤を含有する、請求項1~11のいずれか一項に記載の医薬組成物。
- さらに緩衝剤を含有する、請求項1~12のいずれか一項に記載の医薬組成物。
- pHが4~8である、請求項1~13のいずれか一項に記載の医薬組成物。
- 緑内障又は高眼圧症の治療に用いられる、請求項1~14のいずれか一項に記載の医薬組成物。
- 請求項1~15のいずれか一項に記載の医薬組成物と、マルチドーズ型容器と、を備える製品。
- チモロール又はその塩を含有し、防腐剤を含まない又は所定量で含み、
前記所定量は、該防腐剤及び水からなる試験試料中において、エスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記試験試料を遮光下において20~25℃で保存してから7日経過後において、前記試験試料をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下となる量であり、
ホウ酸又はその塩の含有量が、0.001%(w/v)未満であり、
繰り返し使用可能な容器に入れられた医薬組成物中に、さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法。 - チモロール又はその塩を含有し、ホウ酸もしくはその塩を含まない又はホウ酸もしくはその塩の含有量が0.001%(w/v)未満であり、繰り返し使用可能な容器に入れられた医薬組成物中に、
さらに、ドルゾラミド又はその塩を含有させることによって、防腐効力を向上させる方法であって、
前記ドルゾラミド又はその塩を含有させる前の医薬組成物は、該医薬組成物をエスケリシア・コリ(Escherichia Coli)のATCC 8739の菌液濃度が105~106cfu/mLの範囲内となるように菌を接種して均一に混合し、前記医薬組成物を遮光下において20~25℃で保存してから7日経過後において、前記医薬組成物をマイクロピペットで1mLを採取し、生菌数を測定したときの菌数(A)に対する接種時の菌数(B)の比(B/A)の常用対数値が2.0以下である、方法。
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US20100210720A1 (en) * | 2007-07-20 | 2010-08-19 | Laboratoires Thea | Preservative-free prostaglandin-based ophthalmic solution |
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