WO2016039149A1 - Procédé de détection et appareil de détection - Google Patents

Procédé de détection et appareil de détection Download PDF

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Publication number
WO2016039149A1
WO2016039149A1 PCT/JP2015/074130 JP2015074130W WO2016039149A1 WO 2016039149 A1 WO2016039149 A1 WO 2016039149A1 JP 2015074130 W JP2015074130 W JP 2015074130W WO 2016039149 A1 WO2016039149 A1 WO 2016039149A1
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WO
WIPO (PCT)
Prior art keywords
light
detected
total reflection
angle
liquid
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PCT/JP2015/074130
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English (en)
Japanese (ja)
Inventor
幸登 中村
真紀子 大谷
鶴紀 田村
洋一 青木
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コニカミノルタ株式会社
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Application filed by コニカミノルタ株式会社 filed Critical コニカミノルタ株式会社
Priority to JP2016547350A priority Critical patent/JPWO2016039149A1/ja
Publication of WO2016039149A1 publication Critical patent/WO2016039149A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence

Definitions

  • FIG. 1 is a diagram illustrating a configuration of the SPFS apparatus according to the first embodiment.
  • FIG. 2 is a flowchart illustrating an example of an operation procedure of the SPFS apparatus according to the first embodiment.
  • FIG. 3 is a graph showing the relationship between the incident angle of excitation light and the amount of plasmon scattered light.
  • 4A and 4B are graphs showing the relationship between the incident angle of excitation light and the amount of plasmon scattered light.
  • FIG. 5 is a graph showing the relationship between the incident angle of excitation light and the coefficient of variation.
  • FIG. 6 is a diagram illustrating a configuration of an SPR device according to a modification of the first embodiment.
  • FIG. 7 is a diagram illustrating a configuration of the SPFS apparatus according to the second embodiment.
  • the prism 20 has a considerable amount of birefringence.
  • Examples of the material of the prism 20 include resin and glass.
  • the material of the prism 20 is preferably a resin having a refractive index of 1.4 to 1.6 and a small birefringence.
  • the channel lid 40 is preferably made of a material that is transparent to signals such as fluorescence ⁇ emitted from the metal film 30 and plasmon scattered light ⁇ .
  • An example of the material of the flow path lid 40 includes a resin.
  • the other portion of the channel lid 40 may be formed of an opaque material.
  • the flow path lid 40 is bonded to the metal film 30 or the prism 20 by, for example, adhesion using a double-sided tape or an adhesive, laser welding, ultrasonic welding, or pressure bonding using a clamp member.
  • the first lens 134 is, for example, a condensing lens, and condenses light emitted from the metal film 30.
  • the second lens 136 is, for example, an imaging lens, and forms an image of the light collected by the first lens 134 on the light receiving surface of the first light receiving sensor 137.
  • the optical path between both lenses is a substantially parallel optical path.
  • the optical filter 135 is disposed between both lenses.
  • the control unit 160 calculates the physical property of the specimen (the liquid to be detected), that is, the hematocrit value of the blood, based on the detection result of the light detection unit 130. Specifically, the amount of plasmon scattered light ⁇ of the specimen is calculated by subtracting the reference value from the detection value. Next, the control unit 160 applies the amount of the plasmon scattered light ⁇ of the specimen described above to a calibration curve prepared in advance, and calculates the hematocrit value of blood contained in the specimen. In addition, the control part 160 can also calculate the density
  • the white circle symbol indicates the experimental result when blood having a hematocrit value of 60% is used, and the black triangular symbol indicates the experimental result when blood having a hematocrit value of 43% is used.
  • the white triangle symbol indicates the experimental result when blood with a hematocrit value of 35% is used, and the black circle symbol indicates the experimental result when serum (hematocrit value is 0%) is used. Yes.
  • the SPFS device 100 when the SPFS device 100 according to the present embodiment detects physical properties (particle concentration, particle size, etc., hematocrit value in this embodiment) of a specimen (liquid to be detected) containing particles.
  • the incident angle (first incident angle) of the excitation light ⁇ is made smaller than the incident angle (second incident angle) of the excitation light ⁇ when detecting the amount of the substance to be detected in the specimen.
  • the physical properties (hematocrit value in the present embodiment) of the specimen (the liquid to be detected) and the amount of the substance to be detected in the specimen can be detected with high accuracy, and as a result, the hematocrit value is corrected.
  • the amount of the substance to be detected can be calculated with high accuracy.
  • the SPFS apparatus 100 according to the present embodiment does not require a new apparatus for detecting the hematocrit value. Thereby, the apparatus cost can also be suppressed without increasing the size of the SPFS apparatus 100.
  • the example is shown in which the SPRS device 300 and the SPR device 400 using SPR are used as the detection device, but the analysis chip in which the metal film 30 is not disposed. You may apply to the detection system which uses. In this case, the detection apparatus detects (measures) the total reflection measurement (ATR) method or the like without using SPR, but the same effects as those of the second embodiment and the modification of the second embodiment can be obtained.
  • ATR total reflection measurement

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  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé de détection et un appareil de détection, avec lesquels une propriété physique d'un liquide de détection contenant des particules ou la quantité d'une substance à détecter peut être détectée quantitativement à un rapport S/N élevé avec une grande précision. Le procédé de détection est un procédé pour détecter une propriété physique d'un liquide de détection qui contient des particules. Le liquide de détection est fourni sur une surface de réflexion totale d'une puce d'analyse ayant un prisme comprenant un diélectrique et comprenant la surface de réflexion totale et, avec le liquide de détection présent sur la surface de réflexion totale, la surface de réflexion totale est exposée à de la lumière provenant du côté de prisme à un angle incident égal ou supérieur à un angle critique, de façon à détecter la quantité de lumière diffusée émise par liquide de détection. La propriété physique du liquide de détection est calculée sur la base de la quantité de la lumière diffusée.
PCT/JP2015/074130 2014-09-10 2015-08-27 Procédé de détection et appareil de détection WO2016039149A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2016547350A JPWO2016039149A1 (ja) 2014-09-10 2015-08-27 検出方法および検出装置

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2014-184189 2014-09-10
JP2014184197 2014-09-10
JP2014184189 2014-09-10
JP2014-184197 2014-09-10

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WO2016039149A1 true WO2016039149A1 (fr) 2016-03-17

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018034208A1 (fr) * 2016-08-18 2018-02-22 コニカミノルタ株式会社 Procédé de mesure
WO2018034143A1 (fr) * 2016-08-18 2018-02-22 コニカミノルタ株式会社 Procédé de mesure, appareil de mesure et système de mesure
JP2018031730A (ja) * 2016-08-26 2018-03-01 コニカミノルタ株式会社 ヘマトクリット値の測定方法、ヘマトクリット値の測定装置、被測定物質の量の測定方法、および被測定物質の量の測定装置
WO2018051863A1 (fr) * 2016-09-14 2018-03-22 コニカミノルタ株式会社 Procédé de mesurage

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020204540A (ja) * 2019-06-18 2020-12-24 矢崎総業株式会社 金属の腐食検出装置及び腐食検出方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004042403A2 (fr) * 2002-11-04 2004-05-21 Ludwig-Maximilian-Uni Versität München Procedes, dispositif et instrument pour la detection d'analytes
US20050185186A1 (en) * 2004-02-20 2005-08-25 The University Of Maryland Far-field optical microscope with a nanometer-scale resolution based on the in-plane image magnification by surface plasmon polaritons
WO2011152064A1 (fr) * 2010-06-04 2011-12-08 コニカミノルタホールディングス株式会社 Dispositif et procédé d'analyse par fluorescence à résonance de plasmon de surface
WO2012042805A1 (fr) * 2010-09-30 2012-04-05 コニカミノルタホールディングス株式会社 Dispositif de fluorimétrie par résonance plasmonique de surface et procédé de fluorimétrie par résonance plasmonique de surface
WO2014061743A1 (fr) * 2012-10-18 2014-04-24 コニカミノルタ株式会社 Procédé de dosage faisant appel à la spectroscopie de fluorescence par exaltation plasmonique de surface

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5640873B2 (ja) * 2011-04-07 2014-12-17 コニカミノルタ株式会社 表面プラズモン励起蛍光計測装置及び表面プラズモン励起蛍光計測方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004042403A2 (fr) * 2002-11-04 2004-05-21 Ludwig-Maximilian-Uni Versität München Procedes, dispositif et instrument pour la detection d'analytes
US20050185186A1 (en) * 2004-02-20 2005-08-25 The University Of Maryland Far-field optical microscope with a nanometer-scale resolution based on the in-plane image magnification by surface plasmon polaritons
WO2011152064A1 (fr) * 2010-06-04 2011-12-08 コニカミノルタホールディングス株式会社 Dispositif et procédé d'analyse par fluorescence à résonance de plasmon de surface
WO2012042805A1 (fr) * 2010-09-30 2012-04-05 コニカミノルタホールディングス株式会社 Dispositif de fluorimétrie par résonance plasmonique de surface et procédé de fluorimétrie par résonance plasmonique de surface
WO2014061743A1 (fr) * 2012-10-18 2014-04-24 コニカミノルタ株式会社 Procédé de dosage faisant appel à la spectroscopie de fluorescence par exaltation plasmonique de surface

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MOCK J J ET AL.: "Distance-Dependent Plasmon Resonant Coupling between a Gold Nanoparticle and Gold Film", NANO LETTERS, vol. 8, no. 8, pages 2245 - 2252 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018034208A1 (fr) * 2016-08-18 2018-02-22 コニカミノルタ株式会社 Procédé de mesure
WO2018034143A1 (fr) * 2016-08-18 2018-02-22 コニカミノルタ株式会社 Procédé de mesure, appareil de mesure et système de mesure
JPWO2018034208A1 (ja) * 2016-08-18 2019-06-13 コニカミノルタ株式会社 測定方法
JPWO2018034143A1 (ja) * 2016-08-18 2019-06-13 コニカミノルタ株式会社 測定方法、測定装置および測定システム
US11047798B2 (en) 2016-08-18 2021-06-29 Konica Minolta, Inc. Measurement method, measurement apparatus, and measurement system
JP2018031730A (ja) * 2016-08-26 2018-03-01 コニカミノルタ株式会社 ヘマトクリット値の測定方法、ヘマトクリット値の測定装置、被測定物質の量の測定方法、および被測定物質の量の測定装置
WO2018051863A1 (fr) * 2016-09-14 2018-03-22 コニカミノルタ株式会社 Procédé de mesurage
JPWO2018051863A1 (ja) * 2016-09-14 2019-06-27 コニカミノルタ株式会社 測定方法
US11169089B2 (en) 2016-09-14 2021-11-09 Konica Minolta, Inc. Surface plasmon resonance measurement method for measuring amount of substance in a specimen including whole blood

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JP6587024B2 (ja) 2019-10-09
JPWO2016039149A1 (ja) 2017-06-22
JP2019032342A (ja) 2019-02-28

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