WO2016009981A1 - 有害節足動物誘引化合物と天敵の組み合わせ物 - Google Patents

有害節足動物誘引化合物と天敵の組み合わせ物 Download PDF

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WO2016009981A1
WO2016009981A1 PCT/JP2015/069991 JP2015069991W WO2016009981A1 WO 2016009981 A1 WO2016009981 A1 WO 2016009981A1 JP 2015069991 W JP2015069991 W JP 2015069991W WO 2016009981 A1 WO2016009981 A1 WO 2016009981A1
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mites
methyl
natural enemies
compound
thrips
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PCT/JP2015/069991
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English (en)
French (fr)
Japanese (ja)
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圭 河津
敦雄 水口
祐美子 野口
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協友アグリ株式会社
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A01N63/27Pseudomonas
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    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • AHUMAN NECESSITIES
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    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • A01N63/14Insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A01N63/16Arachnids
    • AHUMAN NECESSITIES
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40Viruses, e.g. bacteriophages

Definitions

  • the present invention synergistically combines an attracting compound for attracting harmful arthropods, and natural enemies against harmful arthropods in harmful arthropods, thereby preventing crops from harmful arthropod damage.
  • the present invention relates to protective control methods and predictive methods to protect, or combinations for use in these methods. Also, a method for controlling the behavior of harmful arthropod populations via such attracting compounds and natural enemies, especially for controlling and predicting the damage of plants by harmful arthropods in institutional greenhouses and in open fields, or Relates to a combination for use in these methods.
  • Damage to cultivated crops by harmful arthropods is a global problem. Some harmful arthropods also carry viruses, causing more serious damage to crops by causing viral diseases.
  • control of harmful arthropods that occur and cause damage to agricultural crops is often carried out with chemical pesticides.
  • minute harmful arthropods that inhabit closed places such as cormorants, it is difficult to detect at low density and control with chemical pesticides.
  • the control effect is lowered due to the emergence of resistance to chemical pesticides, and the environmental impact of the use of chemical pesticides is regarded as a problem. Therefore, there is a need for a method for detecting harmful arthropod populations and reducing the density of harmful arthropod populations in order to reduce damage to cultivated crops and plants.
  • Patent Document 1 In order to use host plants, harmful arthropods use volatile substances produced and released by plants. Accordingly, some of the plant-derived compounds act as attractants for harmful arthropods, and it is possible to attract harmful arthropods using an appropriate trap to which attractants are added (Patent Document 1).
  • Patent Document 2 Non-Patent Document 1, Non-Patent Document 2).
  • a substance produced by a certain organism is disadvantageous to the produced species, but kairomones are known as substances that have a beneficial effect on other species of organisms that have been received.
  • a substance produced by a plant and attracted by an accepted herbivore is a kairomone, and these substances are plant-derived kairomones.
  • Many of the substances found as attracting compounds are aromatic compounds known as plant-derived kairomones.
  • Many plant-derived kairomones have been reported as attractants of various harmful arthropods.
  • Plant-derived kairomones have been reported as attractants for thrips. For example, p-anisaldehyde, ethyl nicotinate, benzaldehyde, o-anisaldehyde, ⁇ -farnesene, eugenol, 3-phenylpropylaldehyde, monoterpenes (geraniol, linalool, nerol, citronellol, eucalyptol), monoterpene ester Class (isobornyl valerate, isobornyl pivalate, lavandulyl valerate) have been reported as attractants of Franklinella occidentalis, but the degree of attraction is different (Non-patent document 3, Non-patent document 4) Non-patent document 5, Non-patent document 6, Patent document 6, Patent document 7, Patent document 8).
  • Non-Patent Document 3 Non-patent document 7, Non-patent document 9).
  • a compound that exhibits kairomone-like activity but is not derived from a plant is sometimes referred to as an analog of a plant-derived kairomone.
  • ethyl nicotinate and methyl m-aminobenzoate are analogs of methyl nicotinate and methyl o-aminobenzoate, which are plant-derived compounds, respectively, and are not plant-derived compounds, but are attractants of thrips (Non Patent Literature 3, Non Patent Literature 5, Non Patent Literature 7, and Non Patent Literature 8).
  • the above substances may show different levels of attractiveness depending on the type of thrips, and have been reported to show species specificity.
  • natural enemies refer to organisms that function as predators or parasites within the biological factors involved in natural control, the power that controls the number of living organisms acting in nature, that is, natural control. In the invention, it is used as having the same concept. Furthermore, pathogenic bacteria and viruses that infect insects and kill directly or indirectly are natural enemies and are called natural enemy microorganisms.
  • Patent Document 10 A method for avoiding damage to crops by predating harmful arthropods has been performed (Patent Document 10). For example, cultivate wheat in a crop cultivation facility to infest parasitoids, then release the natural enemy Koleman abrabachi of wheat beetle and propagate it on the wheat to infest the crops in the cultivation facility. Generally used is a method of killing important harmful arthropods such as cotton aphids and peach aphids to prevent damage.
  • Patent Document 11 For example, in order to protect crops from thrips that are harmful arthropods, Kalanchoe, which is a suitable spawning plant of the common enemies, the domestic enemy, is cultivated inside or outside the crop cultivation facility or field, and the natural enemy There is a method of increasing the population density by increasing the number of the beetles, and effectively suppressing an increase in thrips of harmful arthropods (Patent Document 11).
  • Himehanakamushi (Orius genus) is known as an excellent natural enemy such as thrips, which are difficult-to-control harmful arthropods.
  • As a method for controlling such harmful arthropods Namihimehanamemushi, Tairikhimemekamemushi, etc. Has been tried (Patent Document 13).
  • Patent Document 13 in order to stably supply natural enemies, large-scale breeding indoors has resulted in a new problem that inbreeding weakness due to repeated inbreeding has occurred and the growth rate has decreased.
  • IP M Int eng r a te d P s M a a n e eng ent
  • the “push-pull method” as a pest management technique has been attracting attention in recent years.
  • the “push-pull method” is a decoy by planting corn and pigeons in cotton fields as “decoy crops” to control tobacco moths that harm cotton, and by applying neem oil that pests avoid. It was named because crops attracted (pull) pests and repellents kept pests away (Non-Patent Document 11).
  • the “push-pull method” as a pest management method is intended to control the distribution and density of insect populations in the field by combining factors that stimulate insect behavior such as localization, colonization, feeding, and spawning.
  • the stimulating factor is a factor showing a stimulating effect such as a chemical factor such as a plant-derived kairomone, an allomone, or an insect-derived pheromone, or a physical factor such as a visual stimulus, and a suppressor is a repellent or the like It is a factor that shows the repellent effect.
  • all the methods that combine “stimulating factors” and “suppressing factors” that control insect behavior are push-pull methods.
  • the push-pull method is different from the conventional methods in that the insect population is controlled by simultaneously using the acting factors of the stimulating factor and the inhibitory factor.
  • the push-pull method is a method to maintain the pest population at a low level by utilizing all the factors that control insect behavior and arranging them in the most appropriate design, and comprehensive pest management in a broad sense It can be said that technology.
  • a combination of a stimulating factor and an inhibitory factor works effectively depends on the interaction between the stimulating factor and the inhibitory factor and the position of the main crop. If the attractiveness of the stimulating factor is insufficient, the pest can be attracted to the adjacent main crop and cause damage. Alternatively, if the repellent nature of the suppressor is insufficient, the pests can invade the main crop and the damage to the main crop can be magnified. Thus, there is a problem that using a stimulation factor or a suppression factor by the push-pull method involves a certain risk.
  • an object of the present invention is to provide means for overcoming such disadvantages or problems.
  • the object is to provide at least one compound selected from the group consisting of compounds attracting harmful arthropods and at least one natural enemy selected from the group consisting of natural enemies against the harmful arthropods.
  • One aspect of the present invention is at least one compound selected from the group consisting of compounds having attractiveness to harmful arthropods and at least one compound selected from the group consisting of natural enemies against the harmful arthropods.
  • the place where treatment is required is not particularly limited as long as the desired effect can be achieved in accordance with the purpose of the present invention, but for example, it may be the same space such as in a facility cultivation house and an open field. it can. In this way, a specific attracting substance and a specific natural enemy are used in the same space at the same time to achieve an attraction effect that surpasses conventional techniques, so it is extremely useful for stable production of safe and high-quality agricultural products. It becomes possible to generalize a certain arthropod control method. Furthermore, the present invention is easy for farmers to accept and can minimize the use of chemically synthesized insecticides.
  • the present invention separately relates to a method including a method for attracting harmful arthropods using a compound that has been attracted to harmful arthropods already proposed in the art, and a harmful arthropod
  • the present invention relates to a combined use with a method including a method for repelling the harmful arthropod using natural enemies.
  • Such combined use is carried out in the same space in places where treatment of harmful arthropods is required, for example, methods for controlling harmful arthropods in institutional cultivation houses and outdoor areas, institutional cultivation houses and outdoor areas.
  • the timing of the implementation is not limited as long as the desired effect according to the object of the present invention can be achieved, but one of the methods is performed first, the other method is performed later, and the timing is mutually shifted. Or both may be performed simultaneously.
  • Examples of harmful arthropods to be treated in the method or combination of the present invention include, but are not limited to, for example, thrips, whiteflies, aphids, leafworms, stink bugs, butterflies, and Spider mites can be mentioned. Treatment has been used as a broad concept and generally means that the animal is handled so that an enhanced attraction response of the animal can be achieved.
  • “synergistic action” or “acting synergistically” means at least one compound selected from a specific compound having attraction to a specific harmful arthropod population and a specific harmful node. This means that the attraction effect when combining one or more specific natural enemies with a paw animal population at the same time is significantly higher than the expected effect (so-called additive effect) of the combination.
  • the attraction synergistic effect that is expected when one kind of attracting component compound and one natural enemy are used in combination is determined by the following Colby calculation formula.
  • the “mode of synergistically attracting harmful arthropods” referred to in the present invention is the same space in which the compound having the attractiveness to harmful arthropods and the natural enemy need their treatment. Means that the combination is selected so that the value exceeds the expected value of the attraction rate when calculated in the above equation. Thus, if one or more compounds selected from the group consisting of compounds having attraction to harmful arthropods can be used as the attracting substance as long as the above-mentioned synergistic action of attraction can be confirmed, Any combination of and falls within the scope of the present invention.
  • the compound used as an attracting substance used in the present invention may be any substance that is attractive to the target arthropod.
  • an attracting substance may be any substance that is attractive to the target arthropod.
  • the above attractant is not limited to using only one kind, and two or more kinds of compounds may be used in combination, and the ratio thereof may be any.
  • the combination of two kinds of compounds is not limited, but the weight ratio of the combination of two kinds of compounds is 0.0999 to 99.9001: 99.9001 to 0.0999, preferably It can be 0.1996 to 99.8004: 99.8044 to 0.1996, more preferably 0.99 to 90.01: 90.01 to 0.99.
  • the attractant may be used in combination of three or more compounds, and the ratio thereof may be any.
  • the combination of the three compounds is not limited, but the weight ratio of the combination of the three compounds is 0.0998 to 99.8004: 99.8044 to 0.0998: 0.0.0. 0998 to 99.8004, preferably 0.1992 to 99.6016: 99.616 to 0.1992: 0.1992 to 99.6016, more preferably 0.98039 to 98.03922: 98.03922 to 0.98039 : 0.98039 to 98.03922.
  • the attracting substances used in the present invention are all known compounds, and as these known compounds, commercially available compounds can be used as they are, obtained from commercially available preparations, or synthesized by a known method.
  • the attracting substance used in the present invention may be prepared by dissolving one or more compounds selected from the group of compounds having attraction as it is or by dissolving in an appropriate solvent such as hexane, xylene, acetone, dichloromethane, or the like. It is also possible to use this solution by impregnating it with a suitable carrier or holding body such as paper or cloth. It is also possible to formulate the two or more types of attracting active substances separately and use them in combination at the same location.
  • the attracting substance used in the present invention can efficiently kill harmful arthropods by using it as an attracting source in traps such as adhesive traps, corn traps, funnel traps, and basin traps.
  • the trap using the attracting substance of the present invention as an attracting source can also be used by installing it in a place where harmful arthropods are generated or flying, that is, indoors, facilities, or outdoors.
  • the trap may be used by coloring.
  • the combination of attractant and natural enemy of the present invention can also be used to predict or minimize damage to plants caused by harmful arthropods, or as an aid to such methods.
  • natural enemies used in the present invention include predatory natural enemies, parasitic natural enemies, natural enemy microorganisms, and the like.
  • the natural enemies are predatory natural enemies, such as Swallowsky spider mites (Amblyseius swirskii), Chilean spider mites (Phytoseiulius persimilis), Mite spider mites (Neuseilus caulifornicus), Ipterus tuna (Gypsum spp.) (Amblydromalus limonicus), Kumeliska burid mite (Amblyseius cucumeris), Nicellago burdock mite (Amblyseius esharais), Kousegekaburidani (Euseius sojaensis), Degenerance burdock mite (Ausbius bulgari) nerans), food moth gall midge (Aphidoletes aphidimyza), Thailand Riku Orius (Orius strigicollis), Harmoni
  • Flabus Bacillus simplex, Trichoderma atroviride, Pseudomonas fluorescens, Coniochilium mini It is preferable to contain tans (Coniothyrium minitans), zucchini yellow mosaic virus (Zucchini yellow mosaic virus), and Bacillus thuringiensis (Bacillus thuringiensis).
  • tans Coniothyrium minitans
  • zucchini yellow mosaic virus Zucchini yellow mosaic virus
  • Bacillus thuringiensis Bacillus thuringiensis
  • Cabbage mite (Neoseiulus californicus), Carp mite (Gyneesius liturivorus), Spider mite (Neoseiurus barkeri), Limonica calyx mite (Amblydromalus limonicus) Amblyseius cucumeris, Amblyseius eharai (Euseius sojaensis), Degenerance burdock mites (Amblyseius degenerans), Aphididae (Amblyseius degenerans) Harmonia axyridis, Agricultural thrips (Franklinthrips vespiformis), Akamegashiwaku thrips (Haplotrips brevitubus), Himeka nosago (Propylea japonica) diocoris tenuis, Pyrophorus typicus, yamasoku pheasant (Chrysoperla carnea),
  • the amount of natural enemies released to harmful arthropods used in the present invention is 0.01 to 200 / m 2 , preferably 0.1 to 100 / m 2 in the case of predatory or parasitic natural enemies, More preferably, it can be 1 to 50 heads / m 2 , and in the case of natural enemy microorganisms, 0.1 to 20 kg / ha, preferably 0.5 to 10 kg / ha, more preferably 1 to 5 kg / ha. Can do.
  • the biological control method has an environmental condition in which the growth rate of natural enemies used exceeds the growth rate of harmful arthropods.
  • an environmental condition for example, selecting a cultivation type of a crop adapted to a temperature condition included in a suitable temperature range for development of natural enemies, the temperature condition (specifically, for example, a temperature condition of a growth zero point or higher, It is possible to choose to set the temperature in the facility cultivation house to a temperature that is preferably higher than the flight limit temperature, more preferably higher than the egg-laying limit temperature, and the natural enemy's individual rather than the number of harmful arthropods. The natural increase rate of numbers should be exceeded.
  • information related to the growth zero of the natural enemies and harmful arthropods, the flight limit temperature, the egg-laying limit temperature, etc. are collected and accumulated in advance, and rather than the development of harmful arthropods based on the accumulated information.
  • Appropriate temperature conditions can be selected depending on the growth of the natural enemy.
  • methods for determining whether natural enemy growth rates have environmental conditions that exceed those of harmful arthropods may be empirical or quantitative.
  • Qualitative means as well as means may be used. For example, whether or not the above environmental conditions are met, and the number of natural enemies found per crop (for example, one or more in 10-20 flowers) is investigated and observed. , You may observe and confirm the increase of natural enemies that naturally occurred after the release of natural enemies.
  • this biological control method when releasing natural enemies, it is also preferable to release the natural enemies by releasing them several times, such as about 2 to 3 times, for example, at intervals of one week from the initial stage of occurrence of harmful arthropods.
  • the combination of the attracting substance and the natural enemy can exist by being applied to the facility house or the open ground that are the same space at the same time or in any desired order.
  • the combination of the attractant of the present invention and the natural enemy is based on at least one compound selected from the group consisting of compounds derived from plant-derived kairomones and analogs thereof described below, and natural enemies described below. It can be a combination with at least one natural enemy selected from the group consisting of: As a combination of attractants, 1) Swarsky burdock mites, p-anisaldehyde 2) Swarsky burdock mites, benzaldehyde 3) Swarsky burdock mites, ethyl nicotinate 4) Swarsky burdock mites, geraniol 5) Swarsky burdock mites, linalool 6) Swarsky burdock mites, Nellore 7) Swar 8) Swarsky burdock mite, o-anisaldehyde 9) Swarsky burdock mite, ⁇ -farnesene 10) Swarsky burdock mite, methyl anthran
  • Harmful arthropods can be investigated by counting the number of organisms captured in the trap with the attractant. Based on the estimated population density, the necessity for control is determined. Massive capture of harmful arthropods requires the method of harmful arthropod removal provided by the present invention. Conversely, if the harmful arthropod is of low density, it does not necessarily require rapid control.
  • the attractant of the present invention can be formulated as necessary and placed in a trap.
  • Such traps are designed to release an effective amount of attractant.
  • the trap is installed in a place where harmful arthropods are generated or expected to occur.
  • the odor of the attractant attracts harmful arthropods to the trap, and then, for example, by pre-installing an insecticide that shows a lethal effect on the harmful arthropods in the trap, It is possible to kill harmful arthropods attracted inside. Alternatively, it is possible to attract and kill by installing an adhesive plate in or near the trap. Examples of effective insecticidal active ingredients that can be combined with the attractant of the present invention are described below.
  • Effective insecticidal active ingredient o-ethyl o-4-nitrophenyl phenylphosphonothioate (EPN), acephate, isoxathion, isofenphos, isoprocarb, etrimfos, oxydeprofos, oxydeprofos (Quinalphos), cadusafos, chlorethoxyphos, chlorpyrifos, chlorpyrifos-methyl, chlorofenvinphos, alithifo Difofoton, dimethoate, sulprofos, diazinon, thiomethon, tetrachlorvinphos, tebupyrimfos, tebupirimfos, tebupirimfos vamdotion, pyraclophos, pyridafenthion, pyrimiphos-methyl, fenitrothion, fenthion, phenthate, buthothio
  • insecticidal active ingredients synergists (eg piperonyl butoxide, sesamex sulfoxide), MGK 264, N-decylimidazole, N-decylimidazole, Contains an inducer and a resistant (WARF-antiresistant), TBPT, TPP, IBP, PSCP, CH3I, t-phenylbutenone, diethyl maleate, DMC, FDMC, ETP, ETN
  • synergists eg piperonyl butoxide, sesamex sulfoxide
  • MGK 264 eg., N-decylimidazole
  • N-decylimidazole N-decylimidazole
  • TBPT TPP
  • IBP IBP
  • PSCP CH3I
  • t-phenylbutenone diethyl maleate
  • DMC FDMC
  • Examples of the solid carrier used in formulating the attractant include kaolin clay, attapulgite clay, bentonite, montmorillonite, acid clay, pyrophyllite, talc, diatomaceous earth and calcite, corn cob flour,
  • Examples include a natural organic material such as walnut shell powder, a synthetic organic material such as urea, a salt such as calcium carbonate and ammonium sulfate, a fine powder or a granular material composed of a synthetic inorganic material such as synthetic hydrous hydroxide
  • the liquid carrier includes, for example, xylene , Aromatic hydrocarbons such as alkylbenzene and methylnaphthalene, alcohols such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether, ketones such as acetone, cyclohexanone and isophorone, vegetable oils such as soybean oil and cottonseed oil , Petroleum Aliphatic hydrocarbons, esters, di
  • Surfactants include, for example, anions such as alkyl sulfate esters, alkylaryl sulfonates, dialkyl sulfosuccinates, polyoxyethylene alkylaryl ether phosphate esters, lignin sulfonates and naphthalene sulfonate formaldehyde polycondensates.
  • Nonionic surfactants such as surfactants, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl polyoxypropylene block copolymers and sorbitan fatty acid esters, and cationic surfactants such as alkyltrimethylammonium salts.
  • formulation adjuvants include, for example, water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, gum arabic, alginic acid and its salts, polysaccharides such as CMC (carboxymethyl cellulose) and xanthan gum, aluminum magnesium silicate, Examples include inorganic substances such as alumina sol, preservatives, colorants, and stabilizers such as PAP (isopropyl acid phosphate) and BHT.
  • water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone
  • gum arabic alginic acid and its salts
  • polysaccharides such as CMC (carboxymethyl cellulose) and xanthan gum
  • aluminum magnesium silicate examples include inorganic substances such as alumina sol, preservatives, colorants, and stabilizers such as PAP (isopropyl acid phosphate) and BHT.
  • PAP isopropyl acid phosphate
  • the present invention is based on harmful arthropods (for example, harmful arthropods such as harmful insects and harmful ticks) that attract and capture harmful arthropods and cause feeding, sucking, etc. Plants can be protected from harm.
  • harmful arthropods for example, harmful arthropods such as harmful insects and harmful ticks
  • Plants can be protected from harm.
  • the prediction method and control method using the attractant and natural enemy of the present invention can be used in farmland such as fields, paddy fields, lawns, orchards, or non-agricultural land.
  • the present invention is used to control harmful arthropods in the farmland without causing any phytotoxicity in the farmland where the “plants” listed below are cultivated. Can do.
  • Agricultural crops corn (Zea mays), rice (Oryza sativa), wheat (Triticum aestivum), barley (Hordeum vulgare), rye (Secale cereal), oat (Avena sativa olh, sorghum) (Gossypium arboreum), soybean (Glycine max), peanut (Arachis hypogaea), buckwheat (Fagopyrum esculentum), sugar beet (Beta bulgaris ssp. Vulgaris), rape (rape. (Saccharum officinarum), tobacco (Nicotiana tabacum) and the like.
  • Vegetables Eggplant (Solanum melogena), tomato (Solanum lycopersicum), green pepper (Capsicum annuum var. Angulosum), capsicum (Capsicum annuum), potato (Solanum tubulus cucumber) , Pumpkin (Cucurbita maxima), zucchini (Cucurbita pepo), watermelon (Citullus lanatas), melon (Cucumis melo) etc., cruciferous vegetables (Raphanus sativus var. , Seyo Uwasabi (Armoracia rusticana), kohlrabi (Brassica oleracea var. Gongylodes), Chinese cabbage (Brassica rapa var.
  • Trees other than fruit trees tea (Camellia sinensis), mulberry (Morus alba), flowering trees (Rhodendroni indicum), camellia (Camellia japonica laca), hydrangea macrophylla (Hydrangea macrophylla) Cherry (Cerasus xyedoensis), Liriodendron tulipifera, Salgersberger (Lagerstroemia indica), Kinsokusei (Osmanthus fragrance var.
  • Aurantiacus) typhylla var.japonica dogwood (Benthamidia florida), eucalyptus (Eucalyptus globulus), ginkgo (Ginkgo biloba), lilac (Syringa vulgaris), maple (Acer palmatum), oak (Quercus myrsinaefolia), poplar (Populus angulata), Judas tree ( Cercis chinensis, Fuyu (Liquidambar formosana), Platanus (Platanus orientalis), Japanese zelkova (Zelkova serrata), Kurobe (Thuja standishii), Japanese cypress (Abies figas) ), Juniper (Juniperus rigida), pine (Pinus densiflora), spruce (Picea jezoensis var.
  • Lily Lily (Lilium spp.), Pansy (Viola X wittrocciana), Cyclamen (Cyclamen spp.), Orchidaceae spp., Lily of the valley (Convallaria majlis), Lavender (lavenda laval) oleracea var. acephala f. tricolor, primula (Primula spp. alt), poinsettia (Euphorbia pulcherrima), gladiolus spp., cattleya spm.
  • preferable examples include eggplant, tomato, pepper, capsicum, cucumber, pumpkin, zucchini, watermelon, melon, radish, turnip, cabbage, cabbage, mustard, broccoli, cauliflower, rape, burdock, garlic, artichoke, lettuce , Leek, onion, garlic, asparagus, carrot, parsley, celery, spinach, perilla, mint, basil, strawberry, sweet potato, yam and taro.
  • the “plant” may be a plant to which resistance is imparted by a genetic recombination technique or a breeding method by crossing.
  • a bulb means here a bulb, a bulb, a rhizome, a tuber, a tuberous root, and a root support body.
  • Examples of harmful arthropods for which the combined use of a harmful arthropod attractant and natural enemies according to the present invention exhibit a controlling effect include the following.
  • Species of the outer pods, Pteridopsis, Arthropoda Lalodelphax striatellas, Japanese planthoppers (Nilaparvata lugens, etc.), Japanese planthoppers (Sugatella furcifera), etc.
  • Cotton aphids (Aphis gossypii), peach aphids (Myzus persicae), radish aphids (Brevicoryne brassicae), tulip beetle aphids (Macrosiphum euphorbiae), potato aphid ni), wheat aphids (Rhopalosiphum padi), aphids such as the citrus aphid (Toxoptera citriticidus), Nezara antenatum (Shirasotemushishito), Riptortus (Liptortus) (Eysarcoris parvus), smelt beetle (Halyomorpha mista), stink bugs such as the finished plant bug (Lyus lineolaris), Trialureodes vapariorium, B.
  • Arctiidae such as Kashirohitori (Hyphantria cunea), clothes moth (Tinea translucens) Hirozukoga such as such; Species of Arthropoda: Hemimya antiqua, Hemimya platya, Amymyza oryzae roe, Ms.
  • the leaf fly (Dacus cucurbitae), the fruit fly (Ceratitis capitata) and the like; Inner ⁇ eyes Coleoptera arthropod pests: beetle, Epilachna vigintioctopunctata (Epilachna vigintioctopunctata), cucurbit leaf beetle (Aulacophora femoralis), Kisujinomihamushi (Phyllotreta striolata), Inedorooimushi (Oulema oryzae), rice weevil (Echinocnemus squameus), rice water weevil (Lissorhoptrus oryzophilus), Cotton weevil (Anthonomus grandis), Azuki beetle (Callosobrchuchus chinensis), Shibao weevil (Sphenophorus venatus), Japanese beetle (Popilia japonica), Doganebui
  • the harmful arthropod-inducing substance and natural enemies related to the present invention are preferably harmful arthropods that are effective in controlling the outer enemies.
  • harmful arthropods include upper-fly fly harmful arthropods, tick-eye harmful arthropods, and more preferably outer limbs, thrips-eye harmful arthropods.
  • Test Example 1 Attracting effect when individual attractants or natural enemies are treated p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, ⁇ -farnesene, methyl anthranilate, benzoic acid Selected from methyl acetate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, methyl o-toluate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, ⁇ -hexylcinnamaldehyde A hexane solution containing 1 ⁇ g of each single agent was prepared.
  • Test Example 2 Synergistic effect of attractant and natural enemy p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, ⁇ -farnesene, methyl anthranilate, methyl benzoate, o-aminoacetophenone Weight of 1 ⁇ g of each single agent selected from o-anisidine, methyl m-aminobenzoate, methyl o-toluylate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, ⁇ -hexylcinnamaldehyde A hexane solution containing was prepared.
  • Attracting rate (Number of catches in treated area ⁇ number of catches in untreated area) * 100 / (total number of released insects per cup)
  • the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
  • “Formula 2”; E X + Y ⁇ (X * Y) / 100
  • X Attraction rate when treating active ingredient compound A
  • Y Attraction rate when treating natural enemy B
  • E Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
  • Test Example 3 Attracting effect when individual attractants or predatory natural enemies are treated individually A hexane solution containing 1 ⁇ g of each single agent selected from p-anisaldehyde, nerol and squalene is prepared, and this solution is applied to filter paper. After the impregnation, the filter paper was attached to the central part of the colored adhesive plate installed in the plastic ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of orange thrips captured in the colored sticky trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 3.
  • Test Example 4 Synergistic effect of attractant and predatory natural enemy A hexane solution containing 1 ⁇ g of each single agent selected from p-anisaldehyde, nerol and squalene was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup. At the same time, two adults of the mosquito bug were released in an ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green leaf pieces laid with absorbent cotton containing water were placed as food for the Thrips thrips.
  • Test Example 5 Attracting effect when individual attractants or predatory natural enemies are treated p-anisaldehyde, linalool, citronellol, o-anisaldehyde, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-toluic acid
  • a hexane solution containing 1 ⁇ g of each single agent selected from methyl, eugenol, cinnamaldehyde, eucalyptol, squalene, and ⁇ -hexylcinnamaldehyde was prepared, and this solution was impregnated into filter paper.
  • the filter paper was affixed to the central part of the colored adhesive plate installed in the cream cup.
  • Test Example 6 Synergistic effect of attractant and predatory natural enemy p-anisaldehyde, linalool, citronellol, o-anisaldehyde, methyl anthranilate, methyl benzoate, o-aminoacetophenone, methyl o-toluate, eugenol, cinnamaldehyde, A hexane solution containing 1 ⁇ g of each single agent selected from eucalyptol, squalene, and ⁇ -hexylcinnamaldehyde was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup.
  • Test Example 7 Attracting effect when individually treated with attractant or predatory natural enemy Prepare a hexane solution containing 1 ⁇ g of each single agent selected from o-anisaldehyde, methyl benzoate and o-aminoacetophenone, This solution was impregnated into filter paper. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and the green leaf pieces as food for the spider mite, and place the filter paper containing the attracting compound in the center of the sticky plate Affixed. Thereafter, 15 nymph mites were released in the ice cream cup. After 24 hours, the number of spider mites captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1.
  • Test Example 8 Synergistic effect of attractant and predatory natural enemy A hexane solution containing 1 ⁇ g of each single agent selected from o-anisaldehyde, methyl benzoate and o-aminoacetophenone was prepared, and this solution was impregnated on filter paper. It was. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and the green leaf pieces as food for the spider mite, and place the filter paper containing the attracting compound in the center of the sticky plate Affixed. At the same time, two adult dust mites were released in the ice cream cup, and then 15 nymph mites were released in the ice cream cup.
  • Test Example 9 Attracting effect when individual attractants or parasitic natural enemies are treated individually A hexane solution containing 1 ⁇ g of each single agent selected from ethyl nicotinate, linalool, citronellol, methyl benzoate and cinnamaldehyde was prepared.
  • the filter paper was impregnated with this solution. Place an absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and a green leaf leaf as a aphid bait, and place an attracting compound containing an attracting compound in the center of the sticky plate The filter paper contained was affixed. Thereafter, 15 peach aphids were released in the ice cream cup.
  • Test Example 10 Synergistic effect of attractant and parasitic natural enemy Prepare a hexane solution containing 1 ⁇ g of each single agent selected from ethyl nicotinate, linalool, citronellol, methyl benzoate, and cinnamaldehyde, and impregnate the filter paper with this solution. I let you. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky board on top of this and a green leaf piece as food for aphids, and place the filter paper containing the attracting compound in the center of the sticky board. Affixed.
  • Test Example 11 Attracting effect when individual attracting compounds or natural enemy microorganisms were treated p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, Selected from o-anisidine, methyl m-aminobenzoate, methyl o-toluate, ⁇ -hexylcinnamaldehyde, o-anisaldehyde, ⁇ -farnesene, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene
  • a hexane solution containing 1 ⁇ g of each single attractant compound was prepared.
  • kidney beans were immersed in a solution of each natural enemy microorganism selected from currently commonly used natural enemy microorganisms diluted to each practical concentration and air-dried. Then, it cut
  • Attracting ratio to attracting compound (number of individuals attracted to treatment area ⁇ number of individuals attracted to control) * 100 / (number of tests)
  • Attraction rate to untreated leaf pieces (number of individuals attracted to untreated leaf pieces-number of individuals attracted to control) * 100 / (number of tests)
  • “Attracted by control” in the formula indicates that the leaf pieces not treated with either the attracting compound or the natural enemy microorganisms are attracted to the leaf pieces on one side when they are placed at both ends of the glass tube.
  • Test Example 12 Synergistic effect of attractant compound and natural enemy microorganisms p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, m-amino
  • a hexane solution containing 1 ⁇ g weight was prepared.
  • the present invention it is possible to provide a method capable of effectively controlling harmful arthropods using the harmful arthropod-attracting compound and the harmful arthropod-controlling natural enemy.
  • a proposal method of a pest control system that is extremely useful for stably producing safe and high-quality agricultural products can be made into a general-purpose technology.
  • the invention makes it possible to minimize the use of chemically synthesized insecticidal active compounds.

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EP3099160A4 (en) * 2014-02-02 2017-11-08 WTO Investments, LLC Date-derived attractant and bait station
CN107333765A (zh) * 2017-08-10 2017-11-10 福建省农业科学院植物保护研究所 一种蓟马引诱剂及其应用

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CN116035002B (zh) * 2023-01-10 2024-04-26 河北省农林科学院植物保护研究所 一种提高白星花金龟引诱剂诱杀效果的增效剂及其应用

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