WO2015149655A1 - Procédé de traitement d'une bouteille de culture en vue d'empêcher la suspension de gouttelettes au niveau de l'ouverture - Google Patents

Procédé de traitement d'une bouteille de culture en vue d'empêcher la suspension de gouttelettes au niveau de l'ouverture Download PDF

Info

Publication number
WO2015149655A1
WO2015149655A1 PCT/CN2015/075246 CN2015075246W WO2015149655A1 WO 2015149655 A1 WO2015149655 A1 WO 2015149655A1 CN 2015075246 W CN2015075246 W CN 2015075246W WO 2015149655 A1 WO2015149655 A1 WO 2015149655A1
Authority
WO
WIPO (PCT)
Prior art keywords
bottle
culture
treatment
cell culture
plasma
Prior art date
Application number
PCT/CN2015/075246
Other languages
English (en)
Chinese (zh)
Inventor
任辉
董友玉
余志洋
赵向南
Original Assignee
海狸纳米科技（苏州）有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 海狸纳米科技（苏州）有限公司 filed Critical 海狸纳米科技（苏州）有限公司
Publication of WO2015149655A1 publication Critical patent/WO2015149655A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings

Definitions

  • the invention relates to a method for treating a cell culture bottle.
  • the cell culture bottle treated by the method can prevent the liquid droplet from hanging at the mouth of the bottle or flowing along the wall to the bottle body when pouring out the medium in the bottle.
  • the field of biotechnology is a method for treating a cell culture bottle.
  • the object of the present invention is to solve the above technical problems, and to provide a method for treating a cell culture bottle, wherein the cell culture bottle treated by the method can prevent the culture medium from hanging on the bottle when pouring the medium in the bottle. At the mouth.
  • the present invention provides the following technical solutions:
  • the culture bottle is made of a hydrophilic material and is treated in one of two ways:
  • Method 1 The surface of the bottle made of hydrophilic material is hydrophobized by plasma, corona or high-energy radiation, so that the bottle body forms a hydrophobic surface, and then the hydrophobic bottle mouth is protected and treated.
  • the cell culture surface of the inner wall of the body is subjected to tissue cell culture surface modification treatment;
  • method 2 firstly, the cell culture surface is modified by the surface of the inner surface of the bottle body made of the hydrophilic material, and then the cell culture surface is obtained, and then The cell culture surface of the inner wall of the bottle body is protected, so that the cell culture surface is not exposed to the surface hydrophobic treatment conditions of plasma, corona or high-energy radiation, and finally the plasma, corona or high-energy radiation of the bottle mouth is performed.
  • the surface is hydrophobized to form a hydrophobic surface at the mouth of the bottle.
  • the culture bottle is made of hydrophobic material and is treated by the following steps: firstly, the inner wall of the bottle mouth is protected, so that the inner wall of the bottle mouth is not exposed to the hydrophilic treatment conditions of plasma, corona or high-energy radiation, and then The cell culture surface of the bottle body is subjected to tissue cell culture surface modification treatment.
  • the protective treatment comprises two steps, first providing a covering, and secondly covering the covering to the area to be protected and sealing the area to be protected.
  • the covering comprises an elastic arc-shaped clip with a soft material on both sides of the opening, a sealed bottle cap, a film, a quick-drying glue or a sticker.
  • the method for hydrophobizing the plasma surface is to perform plasma treatment using an alkane gas or a fluorine-containing gas as a working gas, wherein the processing power is 0 to 3000 W, the processing time is 30 S to 60 min, and the gas flow rate is 300. ⁇ 3000 sccm, processing temperature 10 to 75 °C.
  • the fluorine-containing gas includes carbon tetrafluoride.
  • the method for hydrophobizing the corona surface is to use a gas plasma under normal pressure to form a hydrophobic group on the surface to be treated to make the surface to be treated hydrophobic, wherein a fluorine-containing gas is used.
  • Working gas processing power 0-3000W, processing time 30S-60min, gas flow rate 300-3000sccm, processing temperature 10-75 °C.
  • the method of hydrophobization treatment of the high-energy ray irradiation surface is performed by plasma treatment, oxidation is performed, a peroxide layer is formed on the surface to be treated, and then the oxidized surface to be treated is placed in the fluorinated olefin. Irradiation in the gas at room temperature for 2h ⁇ 14h.
  • the hydrophilic material refers to a surface formed of the hydrophilic material, which has a contact angle with water of less than 90°, and includes polymethyl methacrylate or polycarbonate.
  • the hydrophobic material refers to a surface formed of the hydrophilic material, which has a contact angle with water of more than 90°, and includes polystyrene, polypropylene or polyethylene.
  • the culture bottle treated by the method of the invention has the surface of the culture bottle bottle being hydrophobic, and the inner wall of the culture bottle is hydrophilic, suitable for cell culture and growth, so that the culture bottle can It can meet the requirements of cell culture, and can prevent the droplets from hanging on the bottle mouth or flowing along the wall to the bottle body during the use process, thereby avoiding contamination of the contents of the bottle, thereby improving the cell culture efficiency.
  • Figure 1 is a schematic view showing the practical state of a sealed bottle cap in a preferred embodiment of the present invention
  • Figure 2 is a partial enlarged view of A in Figure 1;
  • Figure 3 is a schematic view showing the structure of an elastic circular clip in a preferred embodiment of the present invention.
  • Figure 4 is a schematic view showing the structure of another angle of the elastic circular clip in the preferred embodiment of the present invention.
  • the method for treating the culture bottle for preventing the liquid hanging mouth or flowing along the wall to the bottle body according to the present invention is different according to the material used for the culture bottle.
  • the method includes the following steps: first, the bottle body is subjected to hydrophobic treatment by plasma, corona or high-energy ray irradiation.
  • the plasma treatment is treatment with an alkane gas or a fluorine-containing gas, and the fluorine-containing gas used is preferably carbon tetrafluoride.
  • the hydrophobic bottle mouth is protected, and the cell culture surface of the inner wall of the bottle body is subjected to tissue cell culture surface modification treatment, so that the surface after the treatment is suitable for cell growth.
  • the method may employ any technique known to those skilled in the art, specifically,
  • the tissue cell culture surface modification treatment may refer to a surface treatment by plasma, corona or high energy ray irradiation.
  • the protection process comprises two steps, first providing a cover, secondly covering the cover in the area to be protected and sealing the area to be protected, and the cover can be adhesive tape, ie quick-drying glue Or / and self-adhesive, elastic arc-shaped clip 2 with soft material and other methods to protect. Then, the bottle mouth made of hydrophilic material is subjected to tissue cell culture surface modification treatment by plasma, corona or high-energy ray irradiation, so that the surface of the bottle body is suitable for cell growth.
  • the following method can also be used: first, the inner surface of the bottle body made of the hydrophilic material is subjected to tissue cell culture surface modification treatment to obtain a cell culture surface, and then the inner wall of the bottle body is The cell culture surface is subjected to a protective treatment so that the cell culture surface is not exposed to surface hydrophobizing treatment conditions of plasma, corona or high energy ray irradiation, and the protection treatment also includes two steps, first providing a covering, and secondly, The cover is covered in the area to be protected and the area to be protected is sealed, and the cover can be protected by a film, a quick-drying glue or a self-adhesive method.
  • the surface of the bottle is hydrophobized, and the surface hydrophobic treatment of this step also includes plasma treatment, corona or high energy ray treatment, plasma
  • the treatment is carried out by plasma treatment using an alkane gas or a fluorine-containing gas, and the fluorine-containing gas used is preferably carbon tetrafluoride.
  • the culture bottle is a hydrophobic material, it is only necessary to first protect the inner wall of the bottle mouth as described above, and the cover provided by the protection treatment includes an elastic arc-shaped clip 2, a sealed bottle cap 1, a film, a quick-drying glue or ⁇ and no
  • the protection of the bottle mouth is preferably an elastic arc-shaped clip 2 and a sealed bottle cap 1, and the elastic arc-shaped clip 2 is provided with a soft material 3 so that the inner wall of the bottle mouth is not exposed.
  • the cell culture surface of the bottle body is subjected to tissue cell culture surface modification treatment to obtain a surface suitable for cell growth.
  • the plasma surface hydrophobization treatment method is a plasma treatment using an alkane gas or a fluorine-containing gas as a working gas, wherein the treatment power is 0 to 3000 W, the treatment time is 30 S to 60 min, the gas flow rate is 300 to 3000 sccm, and the treatment is performed.
  • the temperature is 10 to 75 °C.
  • the hydrophobic surface hydrophobization treatment method is a gas plasma under normal pressure, forming a hydrophobic group on the surface to be treated to make the surface to be treated hydrophobic, wherein a fluorine-containing gas is used as a working gas, and the processing power is 0 to 3000 W.
  • the treatment time is 30S to 60 minutes
  • the gas flow rate is 300 to 3000 sccm
  • the treatment temperature is 10 to 75 °C.
  • the surface of the high-energy ray irradiation surface is treated by a plasma method, and then oxidized to form a peroxide layer on the surface to be treated, and then the oxidized surface to be treated is placed in a gas of fluorinated olefin at a normal temperature for 2 hours. ⁇ 14h.
  • the hydrophilic material means that the surface formed by the hydrophilic material has a contact angle with water of less than 90°, including but not limited to polymethyl methacrylate or polycarbonate.
  • the hydrophobic material means that the surface formed by the hydrophilic material has a contact angle with water of more than 90°, including but not limited to polystyrene, polypropylene or polyethylene.
  • the culture bottle treated by the method of the invention has a hydrophobic surface, and the inner wall of the culture bottle is hydrophilic, and is suitable for cell culture and growth, so that the culture bottle can meet the requirements of cell culture and can During use, the liquid is prevented from being dropped at the bottle mouth or flowing along the bottle wall to the bottle body by transferring the medium in the bottle, thereby avoiding the contaminated bottle in the culture bottle, thereby improving the cell culture efficiency.

Landscapes

  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Treatments Of Macromolecular Shaped Articles (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immunology (AREA)

Abstract

La présente invention concerne un procédé de traitement d'une bouteille de culture. Selon les propriétés du matériau de la bouteille de culture, un traitement de modification, un traitement d'hydrofugation de surface et un traitement de protection sont combinés et appliqués à un traitement de bouteille de culture, ce qui permet de rendre la surface de l'ouverture de la bouteille de culture hydrophobe. La bouteille de culture résultante satisfait aux exigences de culture cellulaire et évite la suspension de gouttelettes au niveau de l'ouverture de la bouteille ou leur écoulement le long de la paroi de la bouteille vers le corps de la bouteille, ce qui évite la contamination dans la bouteille.
PCT/CN2015/075246 2014-03-31 2015-03-27 Procédé de traitement d'une bouteille de culture en vue d'empêcher la suspension de gouttelettes au niveau de l'ouverture WO2015149655A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201410124821.6A CN104946529A (zh) 2014-03-31 2014-03-31 一种防液滴挂口的培养瓶的处理方法
CN201410124821.6 2014-03-31

Publications (1)

Publication Number Publication Date
WO2015149655A1 true WO2015149655A1 (fr) 2015-10-08

Family

ID=54161597

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2015/075246 WO2015149655A1 (fr) 2014-03-31 2015-03-27 Procédé de traitement d'une bouteille de culture en vue d'empêcher la suspension de gouttelettes au niveau de l'ouverture

Country Status (2)

Country Link
CN (1) CN104946529A (fr)
WO (1) WO2015149655A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114836323A (zh) * 2022-06-10 2022-08-02 苏州赛普生物科技有限公司 一种等离子体改性细胞培养板的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006099127A1 (fr) * 2005-03-14 2006-09-21 Corning Incorporated Flacon de culture cellulaire
CN201003056Y (zh) * 2007-02-12 2008-01-09 袁建华 细胞培养瓶

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100345616C (zh) * 2006-01-13 2007-10-31 浙江大学 电晕诱导接枝进行聚合物微孔膜改性的方法
CN101586077A (zh) * 2008-05-21 2009-11-25 袁建华 新型超滤膜细胞培养器及其制造方法
CN102276866B (zh) * 2011-07-18 2012-10-03 海狸（广州）生物科技有限公司 一种在细胞培养表面接枝羧基的等离子体-光化学方法
CN203174104U (zh) * 2013-01-30 2013-09-04 海狸纳米科技（苏州）有限公司 一种新型结构的细胞培养瓶

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006099127A1 (fr) * 2005-03-14 2006-09-21 Corning Incorporated Flacon de culture cellulaire
CN201003056Y (zh) * 2007-02-12 2008-01-09 袁建华 细胞培养瓶

Also Published As

Publication number Publication date
CN104946529A (zh) 2015-09-30

Similar Documents

Publication Publication Date Title
JP2017192936A5 (fr)
JP2016222526A5 (ja) グラフェンを含む膜の作製方法
CN101665174A (zh) 一种热熔胶铝箔封口膜及其制造方法
WO2015149655A1 (fr) Procédé de traitement d'une bouteille de culture en vue d'empêcher la suspension de gouttelettes au niveau de l'ouverture
TWI431414B (zh) 微影用防塵薄膜組件
EA201492087A1 (ru) Способ текстурирования на подложке, имеющей большую поверхность
SE8007190L (sv) Forfarande for preparering av glasbehallare for plastforseglingar
Hawkeye et al. Introduction: Glancing angle deposition technology
JP5582729B2 (ja) フッ素樹脂フィルムの改質方法
TW200947152A (en) Pellicle
CN104810317A (zh) 晶圆夹具及晶圆深槽腐蚀方法
CN104393206B (zh) 检测锂离子电池的铝塑膜封边的连接密封性的方法
TW201916935A (zh) 氧化鋅奈米膠囊及其製作方法
CN202875381U (zh) 一种新型超声探头无菌罩
TWI716697B (zh) 光蝕刻用防護薄膜、防護薄膜組件、光罩及曝光方法
CN206692316U (zh) 缓释发生装置及杀菌消毒装置
JPH07237927A (ja) フランジ部付石英ガラス製器具
CN205874410U (zh) 一种具有通气功能的培养皿盖子
CN213800926U (zh) 一种植物sod制品的包装容器
CN202620217U (zh) 一种橡胶瓶塞
CN202235399U (zh) 真空采血管安全头盖
CN204223383U (zh) 一种带有单向阀的折叠瓶
TWI537191B (zh) The sealing structure of the bottle
CN210654337U (zh) 一种棉签袋
CN204274674U (zh) 一种防菌多功能隔离保净袋

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15774374

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase
122 Ep: pct application non-entry in european phase

Ref document number: 15774374

Country of ref document: EP

Kind code of ref document: A1