WO2015135266A1 - Réservoir de coulée d'échantillons pour bandelettes réactives, analyseur de séchage et procédé de coulée et de nettoyage d'échantillon correspondant - Google Patents

Réservoir de coulée d'échantillons pour bandelettes réactives, analyseur de séchage et procédé de coulée et de nettoyage d'échantillon correspondant Download PDF

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Publication number
WO2015135266A1
WO2015135266A1 PCT/CN2014/080940 CN2014080940W WO2015135266A1 WO 2015135266 A1 WO2015135266 A1 WO 2015135266A1 CN 2014080940 W CN2014080940 W CN 2014080940W WO 2015135266 A1 WO2015135266 A1 WO 2015135266A1
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WO
WIPO (PCT)
Prior art keywords
sample
test strip
tank
reaction tank
cleaning
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Application number
PCT/CN2014/080940
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English (en)
Chinese (zh)
Inventor
丁建文
周丰良
Original Assignee
爱威科技股份有限公司
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Filing date
Publication date
Application filed by 爱威科技股份有限公司 filed Critical 爱威科技股份有限公司
Publication of WO2015135266A1 publication Critical patent/WO2015135266A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00108—Test strips, e.g. paper

Definitions

  • the invention relates to the technical field of medical in vitro diagnostic equipment, in particular to a test strip rinsing tank, a drying analyzer and a rinsing and cleaning method thereof. Background technique
  • test strips In the in vitro diagnostic equipment for the test strips, there are three types of test strips and sample processing methods.
  • the first type of treatment is to use a precision syringe to control the amount of spotting, and directly place the drop of the sample on the test strip of each item attached to the test strip.
  • This type of processing method has a simple structure, and the sample can be completed by using a sample needle that can be moved up and down and the precision syringe, and since the sample is added to each test paper pad one by one, the between the test paper pads is avoided. Mutual pollution.
  • the first type of loading method also has many defects.
  • one-to-one loading adds a lot of mechanical actions to the instrument, thereby reducing the detection speed.
  • long-term loading causes mechanical wear to affect the positioning accuracy of the needle.
  • the urine is not added to the test paper pad to affect the result.
  • the diameter of the needle is small (0.6mm or less). For such a small needle diameter, due to the urine The occurrence of crystallization often causes the clogging of the sample needle to cause the instrument to malfunction, or the uneven loading of the sample, resulting in insufficient sample amount of the test paper pad and false negative.
  • the second type of treatment is to use a precision syringe to control the leaching and deposit the sample on the test strip in the disposable test strip.
  • This disposable test strip has a small amount of sample buffer space inside, which can be compared with the spotting method. . Enter more samples.
  • the treatment method of the treatment method is simple, and the leakage spot can be reduced to a certain extent, and the disposable test paper tank is used at the same time, and the test paper tank is not required to be cleaned.
  • the sample is directly immersed in a test tube containing the sample, and then the test strip is taken out and the test strip is transferred to the test portion.
  • the processing method has a simple structure and no leaks.
  • test strip has a certain length. To ensure that it is completely immersed, it must be ensured that the sample size must be large. It is not controllable in actual operation. I don't know if it is completely soaked, if the test strip does not. Being completely immersed will cause some items above to be inaccurate. When the test strip is taken out after soaking, there are many residual samples on the test strip, which will spill around and affect the hygiene of the instrument.
  • the embodiment of the invention provides a test strip splicing groove to ensure that the reaction of the test strip is accurate and sufficient to improve the detection accuracy; the invention also provides a drying analyzer; the invention also provides a Drying analyzer leaching and cleaning methods.
  • a test strip rinsing tank includes a reaction tank for accommodating a test strip; an openable and closable negative pressure chamber communicating with the reaction tank is disposed under the reaction tank; the reaction tank and the negative chamber A drainage hole connecting the two is provided between the pressure chambers.
  • the support end surface of the reaction tank is provided with a plurality of protrusions for the test strip, and the plurality of recessed recesses communicate with each other.
  • the drain hole is provided in the recess between any two of the projections.
  • a plurality of the drainage holes arranged along the width direction of the reaction tank are disposed between any two of the protrusions, and the drainage holes communicate with the reaction tank And the negative pressure chamber, wherein the drainage hole is provided with a plurality of radial thin grooves at an inlet communicating with the reaction tank.
  • the test strip sampling tank Preferably, in the test strip sampling tank, three of the drainage holes are arranged between the two of the protrusions along the width direction of the reaction tank, and the diameter of the drainage hole is 0.5 mm. -1.5mm.
  • a plurality of pipe joints connected thereto are disposed at a bottom of the negative pressure chamber, and a residual liquid lead-out pipe is connected to the pipe joint, and the remaining liquid is provided on the pipeline
  • a solenoid valve for controlling its on-off and a waste liquid pump for providing drainage power
  • a waste liquid tank is disposed at the end of the remaining liquid outlet pipe.
  • a drying analyzer comprising a sample needle and a test strip reaction tank, wherein the test strip reaction tank is a test strip rinsing tank according to any one of the above items.
  • a cleaning tank for accommodating the cleaning liquid and a test tube for accommodating the sample, and a cleaning liquid piping system connected to the sampling needle through the one-way valve are further included.
  • the first movement mechanism for controlling the horizontal and vertical movement of the sample needle in the direction of the test strip, and the driving of the test strip to the reaction tank and the test strip A second movement mechanism of the detection unit.
  • a method for rinsing and cleaning a dry analyzer comprising the steps of:
  • the first motion mechanism drives the sample needle to the position of the test tube, and after quantitatively inhaling the sample in the test tube, the sample needle is driven to the top of the sample strip of the test strip on which the dried test strip has been placed; 2) The first motion mechanism drives the sample needle to move along the length of the test strip, and at the same time, the sample needle is hooked out to the test strip in the test strip at the set speed;
  • test strip After the test strip fully absorbs the sample, open the solenoid valve and the waste liquid pump, and take out the remaining sample in the test strip rinsing tank;
  • the second moving mechanism drives the test strip after absorbing the sample to the test strip detecting unit to detect;
  • the cleaning step is further included, and the first moving mechanism drives the sample needle to move to the cleaning tank to drain the residual sample in the sample needle and carry out the sputum. Cleaning of the inner and outer walls of the sample needle, the cleaning step is performed simultaneously with steps 3) and 4). After the cleaning is completed, the first motion mechanism drives the sample needle to the top of the test strip and rotates to step 5).
  • the test strip splicing tank provided by the invention comprises a reaction tank for accommodating the test strip; the openable and closed negative pressure chamber connected with the reaction tank is arranged below the reaction tank; and the reaction tank and the negative pressure chamber are provided with communication The drainage holes of both.
  • the test strip is placed in the reaction tank.
  • the negative pressure chamber is closed, and then the sample is placed in the reaction tank so that the test strip is completely soaked by the sample.
  • the negative pressure chamber is opened, and the sample in the reaction tank is completely extracted into the negative pressure chamber through the drainage hole, thereby discharging the sample in the rinsing tank and completely immersing through the test strip.
  • Figure 1 is a front cross-sectional view of a test strip splicing groove provided by the present invention
  • Figure 2 is a plan view of Figure 1;
  • FIG. 3 is a schematic structural view of a drying analyzer provided in the present application. detailed description
  • the embodiment of the invention discloses a test strip splicing groove, which ensures the reaction of the test strip is accurate and sufficient to improve the detection accuracy; the invention also provides a drying analyzer; the invention also provides a Drying analyzer leaching and cleaning methods.
  • FIG. 1 is a front cross-sectional view of a test strip splicing groove provided by the present invention
  • FIG. 2 is a top view of FIG. 1
  • FIG. 3 is a schematic structural view of the dry chemistry analyzer provided in the present invention.
  • the invention provides a test strip sump, comprising a reaction tank 31 for accommodating a test strip; below the reaction tank 31, an openable and negative pressure chamber 32 communicating with the reaction tank 31 is provided; the reaction tank 31 and A drain hole 33 communicating between the negative pressure chambers 32 is provided.
  • the test strip is placed in the reaction tank 31, and when the test strip is sprinkled by the test strip, the negative pressure chamber 32 is closed, and then the sample is placed in the reaction tank 31, so that the test strip is sampled.
  • the negative pressure chamber 32 is opened, and the sample in the reaction tank 31 is completely extracted into the negative pressure chamber 32 through the drainage hole 33, thereby extracting the sample in the rinsing tank 3 and passing through
  • the method of completely immersing the test strip makes the sample and the test strip react accurately and fully, and completely eliminates the sample through the setting of the negative pressure chamber, thereby completely avoiding the occurrence of inaccurate spotting, leaking points, excessive leaching, etc.
  • the wrong result of the test strip detection has achieved the purpose of improving the accuracy of the test.
  • the support end surface of the reaction tank 31 is provided with a plurality of protrusions 36 of the overhead test strip, and the plurality of recesses 37 between the plurality of protrusions 36 are in communication with each other.
  • the test strip has a long strip structure, and the support end surface of the reaction tank is a strip reaction tank adapted to the strip structure of the test strip.
  • the support end surface of the reaction tank 31 is provided with a plurality of protrusions 36 of the overhead test strip, and the support end surface of the reaction tank 31 is the inner bottom surface of the reaction tank 31, and the reaction tank 31 supports the test strip while accommodating the reaction sample.
  • the test strip is erected by the protrusion 36, so as to prevent the test strip from directly adhering to the inner bottom surface of the reaction tank 31, and the sample strip is attached to the support end surface of the reaction tank 31 during the process of avoiding the sample discharge, so that the sample cannot be sampled.
  • Reaction tank 31 A plurality of protrusions 36 are disposed therein, and a plurality of protrusions 36 are formed between the protrusions 36.
  • the recesses 37 communicate with each other to facilitate the flow of the sample and the discharge to the drainage holes 33.
  • the projection 36 is a support structure of a strip-shaped structure arranged along the width direction of the reaction vessel 31 to achieve stable support and limit of the test strip.
  • a recess 33 is provided in the recess 37 between any two projections 36.
  • a plurality of protrusions 36 for erecting the test strips are disposed in the reaction tank 31, corresponding to the plurality of protrusion structures, and between any two protrusions 36, drainage is provided between both ends of the reaction tank 31 and the protrusions 36.
  • the hole 33 after the negative pressure chamber 32 is opened, the sample can flow out from the corresponding position of the drainage hole 33, thereby speeding up the discharge speed of the sample, and also facilitating the complete discharge of the sample at different positions of the test strip, and avoiding the test caused by excessive leaching. The wrong result of the strip detection.
  • a plurality of drainage holes 33 are arranged between the two protrusions 36 along the width direction of the reaction tank 37.
  • the drainage holes 33 communicate with the reaction tank 31 and the negative pressure chamber 32, and the drainage holes 33
  • a plurality of radially narrow grooves 35 are provided at the entrance of the communication reaction tank 31.
  • the reaction tank 31 has a certain groove width.
  • the drainage holes 33 are also provided in plurality in the width direction of the reaction tank 31, thereby further increasing the velocity of the sample flowing into the negative pressure chamber.
  • the reaction tank is further provided with a plurality of narrow grooves 35 communicating with the inlet of the drainage hole 33 at the side of the reaction tank 31, and the narrow groove 35 communicates with the drainage hole 33 and is radially distributed in the reaction tank 31 with the drainage hole 33 as an origin.
  • the narrow groove 35 is radially connected to the drainage hole, so that the sample in the reaction tank 31 can be concentrated to flow out through the flow guiding hole 33 to ensure complete discharge of the reaction sample.
  • any two projections 36 are provided with three drainage holes 33 arranged along the width direction of the reaction vessel 31, and the diameter of the drainage holes 33 is 0.5 mm - 1.5 mm.
  • the drainage holes 33 are provided in three, and are covered with a plurality of fine grooves 35 in the width direction of the reaction tank 31, which is more advantageous for the discharge of the sample.
  • the drain hole 33 is provided as a through hole of 0.5 mm to 1.5 mm.
  • the number and size of the drainage holes 33 can be set according to the actual application environment of the test strips, and set according to the following principles.
  • the negative pressure chamber has no negative pressure
  • the excess sample liquid will not be automatically reacted.
  • the groove 31 leaks into the negative pressure chamber 32, on the one hand, ensuring sufficient contact with the test strip after the sample is poured in.
  • reaction on the other hand, when cleaning the rinsing tank, it can ensure that the cleaning liquid component contacts the rinsing tank to ensure the cleaning effect; when the negative pressure chamber 32 generates negative pressure, the excess sample liquid should be quickly poured into the negative pressure chamber. 32.
  • the bottom of the negative pressure chamber 32 is provided with a plurality of pipe joints 34 communicating therewith, and the pipe joint 34 is connected with a residual liquid outlet pipe 7 , and the remaining liquid outlet pipe 7 is provided with a control passage thereof.
  • the broken solenoid valve 5 and the waste liquid pump 6 for supplying the liquid power are provided with a waste liquid tank 8 at the end of the remaining liquid take-out line 7.
  • a plurality of pipe joints 34 are disposed at the bottom of the negative pressure chamber 32, the pipe joint 34 is connected with the residual liquid outlet pipe 7, and the residual liquid pumping pipe 7 is provided with a solenoid valve 5 and a waste liquid pump 6, and the waste liquid pump 6 provides discharge samples or cleaning.
  • the power of the liquid, the solenoid valve 5 controls the opening and closing of the waste liquid pump 6 and the negative pressure chamber 32, and the negative pressure of the negative pressure chamber 32 is supplied through the waste liquid pump 6, to provide working power, and the waste liquid is provided at the end of the remaining liquid lead line 7.
  • the waste liquid pump 6 may be a gas-liquid dual-purpose pump or a vacuum pump
  • the electromagnetic valve 5 is a normally closed type electromagnetic valve.
  • the pipe joint 34 can be provided in plurality or one, and can be designed according to the actual structure of the sprinkling groove.
  • the present invention also provides a drying analyzer comprising a squirting needle 1 and a test strip reaction tank, the test paper provided on the drying analyzer
  • the strip reaction tank is the test strip sprinkling tank 3 provided in the above embodiment.
  • the drying analyzer uses the test strip rinsing tank 3 of the above embodiment, the advantageous effects of the drying analyzer by the test strip rinsing tank 3 can be referred to the above embodiment.
  • the cleaning tank 9 for accommodating the cleaning liquid and the test tube 2 for accommodating the sample are further included, and the sampling needle 1 is connected to the cleaning liquid piping system through the one-way valve.
  • a sample is placed in the test tube 2, and the sample in the test tube 2 is sampled by the sample needle 1 and added to the rinsing tank 3.
  • the sample needle 1 is placed in the cleaning tank 9 to complete the cleaning of the inner and outer walls.
  • the rinsing tank 3 needs to be cleaned after the test is completed to prevent the residual sample from being contaminated by the subsequent sample detection.
  • the sample needle 1 After the sample needle 1 sucks the cleaning liquid, it moves to the top of the rinsing tank, and opens the cleaning liquid pipeline system to open The one-way valve cleaning liquid is discharged into the rinsing tank 3 by the sputum needle 1. After immersing for a period of time, the solenoid valve and the waste liquid pump are turned on, the waste liquid is discharged, and the cleaning rinsing tank is completed.
  • the first movement mechanism for controlling the horizontal and vertical movement of the sample needle 1 in the direction of the test strip 4, and the step of driving the test strip 4 to the reaction tank 31 and the test strip detecting unit are further included.
  • the first moving mechanism can drive the sample needle 1 to move horizontally and vertically in the direction of the test strip, and the horizontal movement causes the sample needle 1 to be displaced between the test tube 2 and the squirting groove 3, and the up and down movement can be sampled in contact with the sample, or close to The rinsing tank 3 completes the leaching work.
  • the second moving mechanism moves the test strip into the reaction tank 31 of the rinsing tank 3, and the test strip 4 is in contact with the sample in the reaction tank 31, and the test strip 4 and the sample are sufficiently ensured by the closing and opening of the negative pressure chamber 32.
  • the reaction is then discharged through the negative pressure chamber 32.
  • the second moving mechanism takes the test strip 4 to the test strip detection unit for detection and finally moves into the waste paper tray.
  • the present invention also provides a method for leaching and cleaning the drying analyzer, comprising the steps of:
  • the first motion mechanism drives the sample needle 1 to the position of the test tube 2, and after the sample in the test tube 2 is quantitatively sucked, the sample needle 1 is driven to the top of the test strip of the test strip 4 where the test strip 4 has been placed.
  • the first motion mechanism drives the sample needle 1 to move horizontally, moves to the position of the test tube 2, and sucks the appropriate amount of the sample in the test tube 2, and then drives the sample needle 1 to move back to the test strip stripping tank 3 where the dried test strip 4 has been placed. Above.
  • the test strip is placed on the raised structure of the reaction tank.
  • the first motion mechanism drives the sample needle 1 to move along the length of the test strip, and at the same time, the sample needle 1 is discharged to the rinsing tank at a set speed. Then, the first moving mechanism drives the sample needle 1 to gradually shift along the length of the test strip. During the movement, the sample needle 1 discharges all the samples according to the preset speed to achieve full contact and reaction between the sample and the test strip.
  • Test strip 4 After fully absorbing the sample, open the solenoid valve 5 and the waste pump 6. Pull out the test strip and leave the remaining sample in the sample tank 3.
  • the solenoid valve 5 and the waste liquid pump 6 are turned on, and the excess sample leaks into the negative pressure chamber 32 and is withdrawn to the waste liquid tank 8 via the waste liquid pump 6.
  • the second motion mechanism drives the test strip 4 after the sample is taken up to the test strip detection unit for detection. After the sample is discharged, the second moving mechanism moves the test strip 4 after absorbing the sample to the test strip detecting unit for detection, and obtains the processing result of the sample.
  • the solenoid valve 5 and the waste liquid pump 6 are closed, and the liquid is connected to the sample needle 1
  • the washing liquid pipeline system starts to feed the cleaning liquid into the reaction tank 31. After a predetermined time, the cleaning liquid thoroughly cleans the inner wall of the reaction tank, and then opens the electromagnetic valve 5 and the waste liquid pump 6, and the cleaning liquid is pumped away to complete the test strip.
  • the cleaning of the sample tank 3 prevents the residual of the sample in the reaction tank 31 and causes cross-contamination between the samples, thereby affecting the subsequent detection results.
  • the first moving mechanism drives the sample needle 1 to the cleaning pool 9, evacuates the residual sample in the sample needle, and performs the inner and outer walls of the sample needle 1 Cleaning.
  • the cleaning liquid pipeline system controls the sampling needle 1 to suck the cleaning liquid, closes the electromagnetic valve 5 and the waste liquid pump 6, and after moving to the top of the sampling tank 3, the cleaning liquid is discharged from the inside of the sampling needle 1 to the sampling tank 3, and the cleaning is scheduled.
  • the waste liquid pump 6 and the solenoid valve 5 are turned on to remove the cleaning liquid to complete the cleaning of the test strip rinsing tank 3.
  • the cleaning step of the sample needle is synchronously performed with steps 3 and 4, after the cleaning is completed, the first motion mechanism drives the sample needle to the top of the test strip sampling slot, and proceeds to step 5), so that each working portion is restored to The predetermined position is convenient for the normal operation of the test.
  • the method completely immersed the test strip of the manual standard operation by immersing the test strip in the rinsing tank, and sucks the excess sample on the test strip, thereby completely avoiding the spotting and the missing point.
  • the erroneous result of the test strip detection caused by excessive leaching, etc. achieves the purpose of improving the detection accuracy.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un réservoir de coulée d'échantillons pour bandelettes réactives (3) comportant un réservoir de réaction (31) destiné à contenir une bandelette réactive (4) ; une cavité à pression négative pouvant être ouverte ou fermée (32) communiquant avec le réservoir de réaction (31) est aménagé sous le réservoir de réaction (31) ; un trou de drainage (33) pour la communication entre le réservoir de réaction (31) et la cavité à pression négative (32) est aménagé entre le réservoir de réaction (31) et la cavité à pression négative (32). La bandelette réactive (4) est placée dans le réservoir de réaction (31), la bandelette réactive (4) est complètement imbibée par un échantillon par coulée de l'échantillon après la fermeture de la cavité à pression négative (32), et une fois que la bandelette réactive est complètement en contact avec l'échantillon, la cavité à pression négative (32) est ouverte afin d'aspirer l'échantillon à l'intérieur de la cavité à pression négative (32) par le biais du trou de drainage (33), et par conséquent, l'échantillon contenu dans le réservoir de coulée d'échantillons pour bandelettes réactives (3) est enlevé par aspiration. Grâce au procédé consistant à imbiber complètement la bandelette réactive (4), l'échantillon réagit précisément et suffisamment avec la bandelette réactive (4) ; de plus, au moyen de l'aménagement de la cavité à pression négative (32), l'échantillon est entièrement enlevé de façon à pouvoir éviter tout résultat de détection de la bandelette réactive (4) erroné à cause d'une application imprécise de l'échantillon, d'une coulée excessive d'échantillon et ou pour d'autres raisons similaires ; et de cette manière, l'objectif d'améliorer la précision de la détection est atteint. L'invention comporte également un analyseur de séchage, et le procédé de coulée et de nettoyage correspondant. L'analyseur de séchage comporte une aiguille de prélèvement (1) et le réservoir de coulée d'échantillons pour bandelettes réactives (3).
PCT/CN2014/080940 2014-03-11 2014-06-27 Réservoir de coulée d'échantillons pour bandelettes réactives, analyseur de séchage et procédé de coulée et de nettoyage d'échantillon correspondant WO2015135266A1 (fr)

Applications Claiming Priority (2)

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CN201410087595.9 2014-03-11
CN201410087595.9A CN103808947B (zh) 2014-03-11 2014-03-11 试纸条淋样槽、干化分析仪及其淋样与清洗方法

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WO2015135266A1 true WO2015135266A1 (fr) 2015-09-17

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CN104142392B (zh) * 2014-08-18 2015-12-09 深圳市领治医学科技有限公司 一种微量样本检测试纸
CN105092865B (zh) * 2015-07-23 2017-07-18 爱威科技股份有限公司 试纸条淋样槽、淋样系统、干化分析仪及其淋样与清洗方法
US10890593B2 (en) * 2015-10-09 2021-01-12 Sysmex Corporation Test piece mounting body, transport unit, and test piece analyzer
CN113907798B (zh) * 2021-09-23 2024-02-23 上海市第十人民医院 一种在手术过程中的便携式pth的检测装置
CN114638807B (zh) * 2022-03-22 2023-10-20 无锡雪浪数制科技有限公司 一种基于深度学习的金属板材表面缺陷检测方法

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CN101464457A (zh) * 2008-07-23 2009-06-24 吕欣龙 液体样本的检测装置
CN103808947A (zh) * 2014-03-11 2014-05-21 爱威科技股份有限公司 试纸条淋样槽、干化分析仪及其淋样与清洗方法
CN203811608U (zh) * 2014-03-11 2014-09-03 爱威科技股份有限公司 试纸条淋样槽及干化分析仪

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