WO2015120472A1 - Compositions dérivées de produits biologiques destinées à être utilisées dans l'agriculture - Google Patents

Compositions dérivées de produits biologiques destinées à être utilisées dans l'agriculture Download PDF

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Publication number
WO2015120472A1
WO2015120472A1 PCT/US2015/015261 US2015015261W WO2015120472A1 WO 2015120472 A1 WO2015120472 A1 WO 2015120472A1 US 2015015261 W US2015015261 W US 2015015261W WO 2015120472 A1 WO2015120472 A1 WO 2015120472A1
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leaves
composition
product
weight
bacillus
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PCT/US2015/015261
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English (en)
Inventor
Jose Alejandro Rodriguez QUINTERO
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Ibex Bionomics, Llc
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Priority claimed from US14/177,015 external-priority patent/US10595535B2/en
Priority claimed from US14/177,203 external-priority patent/US9770036B2/en
Priority claimed from US14/177,199 external-priority patent/US10595536B2/en
Priority to CN201580018762.7A priority Critical patent/CN106535642A/zh
Priority to CA2939188A priority patent/CA2939188A1/fr
Priority to RU2016136350A priority patent/RU2016136350A/ru
Application filed by Ibex Bionomics, Llc filed Critical Ibex Bionomics, Llc
Priority to EP15745856.3A priority patent/EP3104709A4/fr
Priority to JP2016552333A priority patent/JP2017512192A/ja
Priority to AU2015213591A priority patent/AU2015213591A1/en
Priority to BR112016018344A priority patent/BR112016018344A2/pt
Priority to MX2016010348A priority patent/MX2016010348A/es
Publication of WO2015120472A1 publication Critical patent/WO2015120472A1/fr
Priority to IL247222A priority patent/IL247222A0/en
Priority to PH12016501585A priority patent/PH12016501585A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/32—Hydrocarbons, e.g. oil
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

  • BIO-DERIVED COMPOSITIONS FOR USE IN AGRICULTURE of which the following is a specification. BIO-DERIVED COMPOSITIONS FOR USE IN AGRICULTURE
  • This invention relates to compositions and method for controlling plant diseases. More particularly, the invention relates to a composition prepared via microbiological fermentation and a method of use thereof for application to crop plants and soils for controlling plant diseases caused by pathogenic microorganisms.
  • This invention also relates generally to the field of compositions and methods for controlling of pests and pest populations which are known to be having a detrimental effect on human life and human activities.
  • the invention also focuses on the isolation of these biopesticide compositions and formulations that are known to possess pesticidal properties and are derived from natural sources having biological origin which have been fermented with microroganisms.
  • the invention more particularly describes the isolation and characterization, including but not confined to, novel biopesticide compositions possessing pesticidal attributes along with other pharmaceutically important attributes so as to also function as effective bio control agents.
  • the instant invention also relates to bio-pesticidal compositions or agents which exhibit excellent effects and in which there is no risk of pollution being caused thereby; and to a method for the use thereof. More particularly, the present invention relates to bio-pesticidal compositions for agricultural and horticultural use which comprise or consist of fermented products and a carrier for agricultural and horticultural formulation.
  • the present invention also concerns a novel method to treat, prevent and protect plants and agricultural plants from pathogenic attack and pest attacks.
  • the present invention more particularly concerns a method of applying selected fermented products and compositions to a agricultural crops and its locus to treat, prevent and immunize, vis. induce local and systemic resistance of the crop against pesticidal and fungal diseases, wherein such action is referred to in this application as "induced plant defense".
  • Such fermented compositions are applied directly to the plant or soil treat or prevent pesticidal diseases in plants and enhance its own immunization capacity via altering their metabolism.
  • This invention also pertains to products to treat rice diseases such as sheath blight, bacterial panicle blight, the tarsonemid mite and other diseases.
  • the invention also relates to fungicidal compositions and their applications in agriculture, and more particularly to fungicidal compositions that are particularly effective for the prevention of fungal damage and for the treatment of fungal diseases in plants and plant propagation material.
  • Farming is the oldest wealth-creating business known to man.
  • Current scientific strategies to maintain and improve yields in support of high-input agriculture place great emphasis on ' fail- safe' techniques for each component of the production sequence with little consideration of the integration of these components in a holistic, systems approach.
  • Research for sustainable agricultural practices requires a far greater emphasis on such an approach than now is fashionable, despite all the rhetoric given politically to sustainability.
  • Organic famiing uses natural materials which are the by products of the farm and are environmentally safe, it enhances the nutritive qualities of the soil and it nurtures the organisms in the soils, which are generally destroyed by the use of chemical manures and pesticides, and significantly reduces cost. Therefore, at this juncture further work on the development of agricultural biotechnology products based on natural products offers immense potential as viable alternative for sustainable agriculture.
  • Plants have remained central to every civilization as the primary source of life, due to their numerous applications in daily life. Plants are composed of chemical substances of which some are not directly beneficial for the growth and development of the organism. These secondary compounds have usually been regarded as a part of the plants' defense against plant- feeding insects and other herbivores.
  • the pesticidal properties of many plants have been known for a long time and natural pesticides based on plant extracts such as rotenone, nicotine and pyrethrum have been commonly used in pest control.
  • Soil organisms especially bacteria have a key role in determining the rate of organic matter decomposition and thereby nutrient mineralization. These processes determine the rate of nutrient supply to primary producers, largely determining the rate of biomass production and other fundamental ecosystem processes like interactions among different functional groups of organisms that constitute ecosystems. Therefore, elucidation of the mechanisms that determine species composition in plant communities is important.
  • Rhizobacteria once considered passive bystanders of the root environment, are now known to affect plant health, development, and environmental adaptation, both beneficially and detrimentally, and the importance of these bacteria in agriculture is expected to grow. A variety of mechanisms have been identified as being responsible for such plant growth promoting activity.
  • certain microorganisms indirectly promote plant growth by inhibiting the growth of deleterious microorganisms; or directly enhance plant growth by producing growth hormones; and/or by assisting in the uptake of nutrients by the crops, e.g., phosphorus.
  • Plant pests are a major factor in the loss of the world's commercially important agricultural crops resulting both in economic hardship to farmers and nutritional deprivation for local populations in many parts of the world.
  • Broad spectrum chemical pesticides have been used extensively to control or eradicate pests of agricultural importance. There is, however, substantial interest in developing effective alternate pesticides.
  • Bt Bacillus thuringiensis
  • Bt Bacillus thuringiensis
  • Varieties of Bt are known that produce more than 25 different but related ⁇ - endotoxins.
  • Bt strains produce ⁇ -endotoxins during sporulation the use of which is limited because they are active against only a very few of the many insect pests.
  • the limited specificity of the Bt endotoxins is dependent, at least in part, on both the activation of the toxin in the insect gut and its ability to bind to specific receptors present on the insects midgut epithelial cells. Therefore, the ability to control a specific insect pest using ⁇ - endotoxins at present depends on the ability to find an appropriate ⁇ -endotoxin with the desired range of activity. In many cases, no such ⁇ -endotoxin is known, and it is not certain that one even exists.
  • Plants also routinely become infected by viruses, fungi and bacteria, and many microbial species have evolved to utilize the different niches provided by the growing plant.
  • many plant diseases are caused by nematodes which are soil- borne and infect roots, typically causing serious damage when the same crop species is cultivated for successive years on the same area of ground.
  • the severity of the destructive process of disease depends on the aggressiveness of the phytopathogen and the response of the host, and one aim of most plant breeding programs is to increase the resistance of host plants to disease.
  • Novel gene sources and combinations developed for resistance to disease have typically only had a limited period of successful use in many crop- pathogen systems due to the rapid evolution of phytopathogens to overcome resistance genes.
  • Programmed cell death is a process whereby developmental or environmental stimuli activate a genetic program that culminate in the death of the cell. This genetic potential exists in most, if not all, multicellular organisms. In the case of invertebrates, programmed cell death appears to play a dual role by being an integral part of both the insect development process and a response mechanism to infections particularly of viral nature. Programmed cell death appears to be executed in several different manners leading to either apoptosis, atrophy or differentiation.
  • Apoptosis is one of the best characterized types of programmed cell death encompassing cytological changes including membrane-bound apoptotic bodies and cytoplasmic blebbing as well as molecular changes such as endonucleo lysis typified by the generation of oligosomal length fragments.
  • cytological changes including membrane-bound apoptotic bodies and cytoplasmic blebbing as well as molecular changes such as endonucleo lysis typified by the generation of oligosomal length fragments.
  • cytoplasmic vacuolization and swelling rather than condensation seem to be the cytological features associated with apoptotic processes.
  • the novel class of products disclosed within the present invention may also induce programmed cell death and exert a pesticidal effect.
  • biopesticides are roughly classified into plant extracts, microorganisms, natural enemies, natural bioactive substances, fermentation products of certain plant materials and genetically modified organisms (GMO). Bio-pesticides can be safer, more biodegradable, and less expensive to develop than synthetic chemical pesticides.
  • GMO genetically modified organisms
  • Plants are exposed to many microbes, including bacteria, viruses, fungi, and nematodes. Although many of the interactions between these microbes and plants are beneficial or innocuous, many of the interactions are harmful to the plants. Diseases of agricultural crops, ornamental plants, forests, and other plants caused by such plant pathogens, particularly bacterial pathogens, are a worldwide problem with enormous economic impact.
  • pathogenic species of bacteria There are many pathogenic species of bacteria, fungi, and nematodes. Diseases caused by fungal species include pre- and post-emergence seedling damping off, hypocotyl rots, root rots, crown rots, and the like. Pathogenic nematodes cause diseases such as root galls, root rot, stunting, and various other rots. Some nematodes also function as vectors of plant viruses.
  • Pseudomonas and Xanthomonas species affect a large number of different crops.
  • Pseudomonas syringae causes bacterial speck of tomato
  • Xanthomonas campestris pv. malvacearum causes angular leaf spot of cotton
  • Pseudomonas solanacearum causes bacterial wilt of potato
  • Pseudomonas tolaasii causes brown blotch disease of cultivated mushrooms.
  • Potatoes and many other crops, such as celery, head lettuce, carrot, Japanese radish, wasabi, tobacco, tomato, cyclamen, Chinese cabbage, and cabbage, are susceptible to the so-called bacterial soft rots.
  • Erwinia carotovora is a soft rot bacterium that softens and rots storage tissues of many plants and is reported to be ubiquitous in soil.
  • the bacterium typically enters plant tissues through injuries caused by insects, wind, tools, and the like.
  • the bacterium invades the site of injury, and if temperature and moisture conditions are suitable, the bacteria rapidly multiply and macerate the tissue.
  • Erwinia bacteria are latent in potato plants, and will preferentially attack the stem and the tubers only after wounding.
  • Potato seed pieces are also susceptible to infection through the cut surfaces. Erwinia carotovora has a substantial impact on the potato industry.
  • antibiotics such as streptomycin
  • metal compounds such as copper- containing Bordeaux mixture
  • malvacearum which causes angular leaf spot of cotton, presently is controlled by treating seeds with mercury-containing compounds and copper sprays.
  • Other Xanthomonas campestris species such as X. campestris pv. vesicatoria and X. campestris pv. campestris, can be seedborne, and there are no effective means for treating the seeds without injury thereto. These chemicals give unsatisfactory control, however, and also kill useful bacteria, contaminate the environment, and cause chemical injuries. Antibiotic-resistant bacteria have also appeared, and the ability of bacteria to transfer multiple drug resistance genes between genera potentially threatens antibiotic treatment of diseases of humans and/or animals.
  • the biological treatment or bioremediation of waste water, soil, oil spills, refinery waste, refinery and waste water treatment sludge contaminated with hydro carbonaceous contaminants, and the like is desirable.
  • These processes depend on natural bacteria or fungi to biodegrade the typically hydrocarbon hydro carbonaceous contaminants, into more environmentally friendly materials (bioremediation) and include, in addition to the well known aerobic and/or anaerobic processes for waste water treatment, processes used for the treatment of oil spills on water, land and the other contaminated substrates mentioned above.
  • Cellulosic and lignin containing materials are often used in the bioremediation of soil and other particulate solid or semisolid substrates, such as sludges.
  • Oil spills, especially on water, are particularly troublesome to treat, as are oil producing well sites contaminated with crude oil.
  • Waste water processes, in addition to producing bioremediated wastewater, also produce contaminated sludge. This sludge must also be treated, to bio degrade the hydro carbonaceous contaminants remaining in it.
  • One or more cellulosic materials such as wood chips and straw, are typically added to the sludge, as what is referred to as an amendment material, and mixed therewith to provide porosity and sites for the bio active bacteria
  • an amendment material When treating land contaminated with hydro carbonaceous material, such materials are mixed in with the land or soil, to form a composted mass in which the hydrocarbons biodegrade into carbon dioxide and water.
  • TSS total suspended solids
  • TVS total volatile solids
  • FOG fats, oils and greases
  • COD chemical oxygen demand
  • BOD biological oxygen demand
  • Accelerated activity bacteria i.e. highly active bacteria have also been used to breakdown certain toxic wastes such as phenolic compounds and chromium by-products.
  • active bacteria In a typical application, active bacteria, after acclimation, are used to treat toxic wastes to produce harmless, easily disposed non-toxic end products. Highly active bacteria have also been used to control or eliminate malodorous aqueous effluents. Malodorous substances such as hydrogen sulfide, ammonia or butyric acid, if broken down or denatured, are essentially odorless.
  • An example of a material which falls in both the classifications of toxic material and malodorous material is hydrogen sulfide which, in its gaseous form or an aqueous solution is both toxic and malodorous.
  • compositions for treating infestations of stable flies in fruit trees It is an additional object of the present invention to provide compositions for treating waste waters generated in agricultural environments associated with harvesting and processing fruits.
  • a still further object of the invention to provide improved products and methods for general bioremediation of soil, including the improvement of soil condition to enhance the ability of soil to support vital plant growth.
  • An additional further object of the invention is the fermentation and propagation of such fermentation of naturally occurring materials with yeast.
  • Figure 1 shows an untreated palm tree that is infected with bud rot.
  • Figure 2 shows a treated palm tree that is now free of bud rot.
  • Figure 3 illustrates the number of heatlhy plants in a field trial for treating plants infested with mokko.
  • Figure 4 shows the average results of treating two varieties of sugar cane with the growth promoter/ripener product of Example 18 compared with a control.
  • Figure 5 describes the results of treating sugar cane variety CPOO-1101 with the growth promoter/ripener product of Example 18 compared with a control.
  • Figure 6 features the results of treating sugar cane variety CP88-1762 with the growth promoter/ripener product of Example 18 compared with a control.
  • Figure 7 show the emergence of new sprouts after treating sugar cane variety CPOO-1101 with the growth promoter/ripener product of Example 18 compared with a control.
  • the present invention provides compositions and methods for many agricultural phytosanitary applications.
  • the compositions are particularly useful for the mitigation and control of viruses, bacteria, fungi, insects and other pests, and/or complexes of the aforementioned, including but not limited to the following: Basidiomycetes, Ustilaginomycetes, Entorrhizomycetidae, Ustilaginomycetidae, Exobasidiomycetidae, Tilletia caries, Uredinio- mycetes (royas), Coleosporium tussilaginis, Puccinia, Cronartium, Hymenomycetes, Exo- basidiales, Exobasidium vaccinii, Auricolariales, Rhizoctonia, Polyporales, Phymatotrichum, Fomitopsis pinicola, Heterobasidion annosum, Agaricales, Armillaria mellea, Armillaria ostoyae, Oomycetes selected from the group consisting
  • compositions of the invention are also useful against bacterial pathogens that attack, consume (in whole or in part), or impede the growth and/or development of plants and/or act as transmission vectors to the plant and/or other plants caused by such bacterial pathogens.
  • the bacterial pathogens include Agrobacterium, Agrobacterium tumefaciens, Erwinia, Erwinia amylovora, Xanthomonas, Xanthomonas campestris, Pseudomonas, Pseudomonas syringae, Ralstonia solanacearum, Corynebacterium, Streptomyces, Streptomyces scabies, Actinobacteria, Micoplasmas, Spiroplasmas and Fitoplasmas.
  • compositions of the invention are also useful for mitigating, controlling and/or eradicating viral pathogens that attack, consume (in whole or in part), or impede the growth and/or development of the plant and/or act as transmission vectors to the plant and/or other plants caused by such viral pathogens.
  • Such viral pathogens include Carlaviridae, mosaic virus of the alamo, Closteroviridae, viruses that attack citrus fruits, Cucumoviridae, Ilarviridae, dwarf virus attacking prunes, Luteoviridae, Nepoviridae, Potexviridae, potato viruses, Potyviridae, Tobamoviridae, tobacco mosaic virus, Caulimoviridae, couliflower mosaic virus, viruses that attack wheat as well as other viruses that attack vegetation and crops.
  • compositions of the invention are also useful in mitigating, controlling and/or eradicating the following insects: Hemipteras, Lepidoptera, Coleoptera, Homoptera, Diptera, Thysanoptera, Hymenoptera, Isoptera and Aptero.
  • the crops and plants that are treated from possible pathogens inflicted by virus, bacteria, fungi, insects and other pests include Anthocerotae, Musci, Hepaticae, Equisetophyta, Lycopodiophyta, Psilophyta, Pteridophyta and Spermatophyta subdivisions of the Plant kingdom but is not limited to the following families: Poaceae (Wheat, Grains, Cereals), Aracaceae (African Palm), Musaceae (Banana, Plantain, Heliconia), Rubiaceae (Coffee Bean), Fabaceae (Legumes), Malvaceae (Cocoa), Bromeliaceae (Pineapple), Solanaceae (Potato, chili), Brassicaceae (Brocoli), Asparagaceae (Yucca), Agavaceae (Agave), Vitaceae (Grape) and Rosaceae (Strawberry).
  • compositions of the invention are for edaphic and foliar applications for a large variety of crops and plants, included but not limited to those listed above, as well as any and all other pathogenic diseases and/or complexes that are encountered in agriculture.
  • pathogen we define any virus, fungus, bacteria, insect and/or pest or vector that affects the plant detrimentally (biologically or economically).
  • complex we define the interaction of one or more of the pathogen(s) to create a disease or detrimental condition (biological or economic) to the plant, animal or microorganism.
  • the invention further describes the isolation and evaluation of pesticidal, biological, biocontrol, ethno botanical, as well as therapeutic properties of these biopesticide compositions and/or biopesticide formulations obtained from fermenting plants capable of serving as effective biocontrol agents and/or pest control management agents.
  • the products of the invention are useful in the following areas:
  • Foliar and edaphically applied products used to supplement plant nutritional elements like nitrogen, phosphorous and potassium as well as mineral elements including but not limited to silicium, calcium, magnesium and manganese. These products can be applied to but not limited to the crops and plants listed above.
  • Stabilization of stomach pH levels in order to prevent creation of stomach acids, which disrupt the digestive flow, preventing the erosion of the stomach mucus membrane and reducing the incidence of ulcers, gastritis, and reducing conditions which have been linked to stomach cancer.
  • Alcoholic substrates to mix with fuels achieving the increase in caloric potential (BTUs), reduction of viscosity, and/or homogenizing the flash points of the different hydrocarbon molecules present in the fuel, in order to increase the power and performance, and reduction of contaminant emissions of the different fuels derived from biological processes (biofuels), and petroleum.
  • the reduction of viscosity of petroleum products and biofuels includes but is not limited to the liquefaction of petroleum and derivatives, oils, bitumen, shale oils, waste oils, and others, expanding their commercial application and/or facilitating their extraction from the field.
  • the invention also presents an alternative product to chemical pesticides, which is a natural organic composition derived from a fermented mixture of plants and carbohydrates.
  • the pesticidal activity of this composition was investigated against Mycosphaerella fijensis causing Sigatoka disease in Cavendish (banana), Ralstonia solanacearum causing Moko disease in Cavendish (banana), Colletotrichum glotoides and Botryodiplodia theobromae causing anthracnose in crops and fruits, tomato yellow leaf curl virus (TYLCV) in tomatoes, Lasiodiplodia theobromae causing soft rot or fruit rot in crops and fruits, Fusarium oxysporum causing Panama wilt in fruits and crops, and many others.
  • the composition is also useful for treating bud rot or crown rot in palm trees as well as diseases in coffee plants.
  • the invention provides a composition comprising the fermentation product of one or more natural products selected from the group consisting of red beans, peas, white rice, yellow corn and mixtures thereof, an inorganic minerals containing phosphorus, calcium, silicon and titanium and strontium, non-iodized salt, potable water and Bacillus Megaterium.
  • the invention is also directed to a biopesticidal composition for treating, mitigating, inhibiting or preventing the development of a plant pathogenic disease
  • a biopesticidal composition for treating, mitigating, inhibiting or preventing the development of a plant pathogenic disease
  • the instant invention also provides a composition and method for bioremediation of environmental materials containing at least one contaminant.
  • the environmental material can be organic (e.g., mulch) and/or inorganic (e.g., sand).
  • the contaminant can be organic (e.g., petroleum hydrocarbons, greases, etc) and/or inorganic (e.g., nitrates).
  • plant part refers to a leaf, stem, root, fruit, seed, tuber, or the like that can be infected or invaded by a plant pathogen.
  • the soil is intended to mean the medium where the plants are planted.
  • plant pathogen refers to a pathogen capable of infecting and/or invading a plant part and causing disease therein.
  • activity means a component or components of fermented products that can be extracted therefrom in an aqueous solvent and exerts an effect of mitigating, ameloriating, treating, preventing and inhibiting growth of a plant pathogen when applied to a plant part and/or soil.
  • bacteria refers to the ability of a substance to increase mortality or inhibit the growth rate of bacteria.
  • biological control is defined as control of a pathogen or insect or any other undesirable organism by the use of a second organism.
  • An example of a known mechanism of biological control is the use of enteric bacteria that control root rot by out-competing fungi for space on the surface of the root.
  • Bacterial toxins, such as antibiotics, have been used to control pathogens.
  • the toxin can be isolated and applied directly to the plant or the bacterial species may be administered so it produces the toxin in situ.
  • treat when used herein with reference to an organic waste refers to contact of the organic waste with a disclosed composition which results in degradation or conversion of the chemical compounds contained within the organic waste.
  • the treatment may involve degradation of the chemical compounds so as to neutralize odorous compounds contained therein and render the organic waste odorless, or conversion of the carbon-compounds or nitrogen fixation so as to increase the nutrient level of the organic waste.
  • the degradation or conversion may be, for example, effected by the enzymes that are secreted by the one or more microorganisms in the disclosed composition.
  • Exemplary enzymes include, but are not limited to, cellulases, amylases, xylanases, galactanases, mannanases, arabanases, -l,3-l,4-glucanases, glucosidases, xylosidases, lipases, hemicellulases, pectinases, proteases, pectin esterases, and the like.
  • bioremediation is one type of decontamination; other types of decontamination are chemical treatment, mechanical removal, and heat reduction.
  • a contaminant is any material that imparts an undesirable, but not necessarily toxic, property to the environmental material; the terms “contaminant' and “pollutant” are used synonymously.
  • the term “environmental material” refers to material to be bioremediated, and is used synonymously with the terms “matrix”, “waste”, “debris", and “spoils”.
  • Culturing The term “culturing”, as used herein, refers to the propagation of organisms on or in media of various kinds.
  • composition is intended to mean a combination of active agents and another compound, carrier or composition, inert (for example, a detectable agent or label or liquid carrier) or active, such as a pesticide.
  • an effective amount is an amount sufficient to affect beneficial or desired results.
  • An effective amount can be administered in one or more administrations.
  • an effective amount is that amount sufficient to ameliorate, mitigate, prevent, stabilize, reverse, slow or delay progression of the target infection or disease states.
  • Fungicidal refers to the ability of a substance to decrease the rate of growth of fungi or to increase the mortality of fungi.
  • Fungus includes a wide variety of nucleated spore-bearing organisms that are devoid of chlorophyll. Examples of fungi include yeasts, molds, mildews, rusts, and mushrooms.
  • Insecticidal As used herein, 'Insecticidal' refers to the ability of a substance to increase mortality or inhibit the growth rate of insects or their larvae.
  • Microbicidal refers to the ability of a substance to increase mortality or inhibit the growth rate of microorganism.
  • Mutant refers to a modification of the parental strain in which the desired biological activity remains similar to that of the parental strain. Mutants or variants may occur in nature without the intervention of man. They also are obtainable by treatment with or by a variety of methods and compositions known to those of skill in the art. For example, parental strains may be treated with a chemical such as N-methyl-N'- nitro-N-nitrosoguanidine, ethylmethanesulfone, or by irradiation using gamma, x-ray, or UV- irradiation, or by other means well known to those practiced in the art.
  • a chemical such as N-methyl-N'- nitro-N-nitrosoguanidine, ethylmethanesulfone, or by irradiation using gamma, x-ray, or UV- irradiation, or by other means well known to those practiced in the art.
  • Nematocidal refers to the ability of a substance to increase mortality or inhibit the growth rate of nematodes.
  • Pesticidal refers to the ability of a substance to decrease the rate of growth of a pest, i.e., an undersired organism, or to increase the mortality of a pest.
  • the products of the invention are useful for many applications in the field of agronomy, environmental remediation, ecology, and many other fields where a green product is desirable.
  • the products of the invention control, mitigate and induce resistance to many pathogens.
  • the products are useful for controlling viruses, bacteria, fungi, insects and other pests, or pest complexes, as defined herein, through edaphic and foliar applications.
  • Specific diseases for which the products are effective include Bud Rot, Crown Rot, Red Ring, Pudricion de Cogollo, Lethal Yellowing and other pathogenic diseases or complexes in all species of palms, including but not limited to ornamental palms, coconut palms, date palms, African Oil Palms, and hybrids thereof.
  • diseases that are treated with the products of the invention include: 1. Moko, Black and Yellow Sigatoka, Erwinia, Nematodes, Picudo, and other pathogenic diseases or complexes in all species of banana and plantains, heliconias, birds of paradise, and other musaceas, and hybrids thereof.
  • Rice Blast Panicle Blight, Fusarium, Vaneo de Arroz, mosaic virus, and other pathogenic diseases or complexes in all species of cereal or grains, including but not limited to rice, wheat, corn, sorghum, and hybrids thereof.
  • crops and plants which are included for treatment with the products of the invention include coffee, cacao, sugar cane, flowers and other ornamental plants, fruits, vegetables, and legumes, including but not limited to soy, peanuts, tomatoes, avocadoes, mangoes, pears, row crops, pastures and grasses, citrus, beets, berries, root and tuber vegetables and/or crops
  • the products of the invention are also useful biopesticides against all manner of insects or other arthropods that attack, consume (in whole or in part), or impede the growth and/or development of the plant, animal or microorganism, and/or act as transmission vectors to the plant, other plants and/or other animals or humans.
  • the products of the invention are useful against arthropods which can be effectively repelled or eradicated by the present compositions include blood-sucking insects such as mosquitoes ⁇ Culex spp.) represented by Anopheles spp. such as Anopheles albimanus, etc., Aedes spp.
  • Aedes aegypti, Aedes albopictus, etc. house mosquitoes ⁇ Culex spp.) such as common mosquito ⁇ Culex pipiens pallens), Culex tritaeniorphynchus, etc., black flies ⁇ Simuliidae), stable flies ⁇ Stomoxyidae), sand flies ⁇ Psychodidae), bitting midge, etc.; and Ixodidae such as Amblyomma, Rhipicephalus, Dermacentor, Ixodes, Haemaphysalis, Boophilus, etc.
  • pathogen we mean any virus, fungus, bacteria, insect and/or pest or vector that affects the plant and soil detrimentally (biologically or economically).
  • complex we define the interaction of one or more of the pathogen(s) to create a disease or detrimental condition (biological or economic) to the plant, animal or microorganism.
  • the products of the invention also find use in bio -industrial applications such as:
  • Bio -remediation applications such as degradation of hydrocarbons, toxic chemicals, organic and inorganic materials, and mineralization of contaminating compounds that affect soils and/or bodies of water, removal or control of odors and vectors resulting from the decomposition of organic wastes from industrial processes, creation of composts, substrates, hummus, soils, and mulches from organic contaminant materials, in improving human health, as fuel additives, as alcoholic substrates to mix with fuels, achieving an increase in caloric potential (BTUs), reduction of viscosity, and/or homogenizing the flash points of the different hydrocarbon molecules present in the fuel, in order to increase the power and performance, and reduction of contaminant emissions of the different fuels derived from biological processes (bio fuels), and petroleum.
  • BTUs caloric potential
  • the products of the invention can also be used for reduction of viscosity of petroleum products and biofuels, includes but is not limited to the liquefaction of petroleum and derivatives, oils, bitumen, shale oils, waste oils, and others, expanding their commercial application and/or facilitating their extraction from the field.
  • the reduction of viscosity, together with the efficiency of combustion results in a reduction of carbon particles contributing to the cleaning or cleansing of the engine, fuel lines, exhaust, and other parts of the combustion system coming, into contact with the additive.
  • the invention provides multiple products which find use in agriculture as fungicides, bactericides, antiviral, as well as in green bioremediation and water purification.
  • the invention provides a composition for treating, inhibiting or preventing the development of a plant pathogenic disease comprising the fermentation product of one or more natural products selected from the group consisting of green peas, red beans, yellow corn, white onions, green onions (scalions), eucalyptus leaves and/or flowers, green lemon peels and rinds, nettle leaves, yucca leaves, nutmeg (interior part), green lemon peel and rinds, nettle leaves, ruda leaves, wormwood leaves (absinthe), green or red peppers (non-spicy), peeled garlic, green leaves of citronella, red beans, mint green leaves, red tomato leaves and fruit, soya leaves and fruits, celery (leaves and branches), basil(leaves), raw oats in hull, oregano leaves, mata-raton leaves (gliricidia sepium (jacquin)), red beans, horse tail fern (Equisetaceae), plantain leaves, basil oil,
  • compositions of the invention are particularly useful as demonstrated in the examples of the invention for uses is biopesticides, bioremediation, water purification, aquaculture and all applications where agricultural crops and soil are threatened by all pests known in nature. Additionally, the compositions of the invention are also particularly useful in controlling odor of agricultural waste and waste waters.
  • microorganisms can be used in making the fermented compositions of the invention. They include Bacillus sp. microorganisms, Pseudomonas sp. microorganisms, Bifidobacterium sp. microorganisms, and Lactobacillus sp. microorganisms, with one of Streptomyces sp. microorganisms or Corynebacterium sp. microorganisms.
  • microroganisms include Streptomyces pactum, Corynebacterium striatum, Bacillus pumilus, Bacillus stearothermophilus, Bacillus brevis, Bacillus cereus, Bacillus subtilis, Bacillus sphearieus, Bacillus licheniformis, Pseudomonas alcaligenes, Pseudomonas marinoglutinosa, Bifidobacterium thermophilus, Lactobacillus casei, Lactobacillus planatarum and Lactobacillus fermentus.
  • the enhancer is a product comprising the fermentation product of one or more natural products selected from the group consisting of red beans, peas, white rice, yellow corn and mixtures thereof, an inorganic mineral containing phosphorus, calcium, silicon and titanium and strontium, non-iodized salt, potable water and Bacillus Megaterium.
  • the above enhancer can be combined with the following products to produce very useful compositions for use as agricultural bio-pesticides and in bioremediation.
  • the enhancer is combined with:
  • a fermentation product of one or more natural products selected from the group consisting of white onions, green onions (scalions), eucalyptus leaves and/or flowers, green lemon peels and rinds, nettle leaves, yucca leaves, nutmeg (interior part), inorganic minerals containing phosphorus, calcium, silicon and titanium and strontium, water and Bacillus Subtillis;
  • Another product of the invention is a composition
  • a composition comprising the fermentation product of one or more natural products selected from the group consisting of mata-raton leaves (gliricidia sepium (jacquin)), red beans, yellow corn, white rice, basil oil, non-iodized salt, inorganic minerals containing phosphorus, calcium, silicon and titanium and strontium, water, saccharomyces cerevisiae, and azotobacter.
  • An additional product of the invention is a composition
  • a composition comprising the fermentation product of one or more natural products selected from the group consisting of garbanzo beans, lentils, barley, oat hulls, citric oil, non-iodized salt, inorganic minerals containing phosphorus, calcium, silicon and titanium and strontium, water, saccharomyces cerevisiae, bacillus megaterium, bacillus subtillis and bacillus lincheniformis .
  • the invention further provides a bio-derived composition useful in controlling agricultural pests and for bioremediation of waste waters comprising: (a) a product of fermenting with 0.01-0.10% by weight of Baker's yeast; a formulation comprising: (i) 1-10% by weight Green Peas, (ii) 1-10% by weight Red beans, (iii) 1-10% by weight Yellow corn, (iv) 1-10% by weight Sorghum, (v) 5-10% by weight Yellow pine sawdust,(vi) 1 -10%> by weight Pine Oil, (vii) 0.1- 0.5%) by weight non-iodized sea salt; and (b) 1-20% by weight of a mixture of spores comprising Bacillus Subtillis spores, Bacillus Aglomerans spores and Bacillus Megaterium spores.
  • the invention further provides a bio-degreasing composition
  • a bio-degreasing composition comprising the fermentation product of one or more natural products selected from the group consisting of garbanzo beans, lentils, barley, oat hulls, citric oil, non-iodized salt, inorganic minerals containing phosphorus, calcium, silicon and titanium and strontium, water, saccharomyces cerevisiae, bacillus megaterium and bacillus pseudomonas.
  • the minerals used in the fermentation processes of the invention include about 10.00- 20.00 ppm Na, 5,000.00-20,000.00 ppm Mg, 100.00-500.00 ppm Al, Si present as silicate of the many elements in the mineral, 20.00-60.00 ppm P, 10.00-30.00 ppm K, 30,000.00-200,000.00 ppm Ca, 50.00-550.00 ppm Ti, 10.00-45.00 ppm Mn, 300.00-1500.00 ppm Fe, 0.20-1.50 ppm Co, 0.5-3.00 ppm Ni, 0.30-5.00 ppm Cu, 0.50-4.00 ppm Zn, 0.5-5.00 ppm As, 200.00-1,000.00 ppm Sr and 5.00-35.00 ppm Ba, as well as many other trace elements commonly found in those minerals.
  • the natural ingredients are cut or reduced to size and mixed with water.
  • yeast Saccharomyces cerevisiae (Fleischman brand) is added to the 500 ml sample taken in the previous step (4), mixed slowly and manually to the full incorporation of yeast.
  • the product is filtered using a 40 micron mesh filter and using a recirculating pump in order to make sure bio film that may be present in excess biomass is removed.
  • the product is filtered several times.
  • the product is then treated with ultraviolet light of a frequency 240-280nm to provide an aseptic product.
  • Other frequencies may be used as long as an aseptic product is obtained.
  • step 10 one could add spores of Bacillus microorganisms or a mixture of microroganisms for further fermentation.
  • the pathogen may be Aspergillus fumigatus, Botrytis cinerea, Cerpospora betae, Curvularia sp., Ganoderma boninense, Geotrichum candidum, Mycosphaerellafijiensis, Phytophthora palmivora, Phytophthora ramorum, Pythium ultimum, Rhizoctonia solani, Rhizopus sp., Schizophyllum sp., Sclerotinia sclerotiorum, Verticillium dahliae, or Xanthomonas axonopodis.
  • the pathogen may be Aspergillus fumigatus, Botrytis cinerea, Cerpospora betae, Curvularia sp., Ganoderma boninense, Geotrichum candidum, Mycosphaerellafijiensis, Phytophthora palmivora, Phytophthora ramorum, Pythium ultimum
  • the host plant is susceptible to disease caused by Ganoderma boninense or Phytophthora palmivora.
  • the host plant is an oil palm plant and the method is effective to inhibit the growth of the plant pathogen. In other embodiments, the method is effective to kill the plant pathogen.
  • compositions to be applied to the vicinity or directly to the plant, such as around the roots, stems, trunk, seed, or leaves of the plant, applied onto such parts of the plant, or injected into such parts of the plant.
  • the composition can be used to treat or sterilize the soil or plant growth medium, by exposing the soil or plant growth medium to the invention compositions, or by direct contact, such as intermixing, with the composition.
  • the invention also provides a method for treating, mitigating and/or preventing an infestation of stable flies (Stomoxys calcitrans) in agricultural fruit farms which method comprises applying to the fruit plant and/or the soil an effective amount of a composition comprising: (a) a product of fermenting a formulation comprising: (i) 1-10% by weight Green Peas, (ii) optionally 1-10% by weight Red beans, (iii) optionally 1-10% by weight Yellow corn, (iv) optionally 1-10% by weight Sorghum, (v) optionally 5-10% by weight Yellow pine sawdust, (vi) optionally 1 -10%> by weight Pine Oil, (vii) 0.1- 0.5%> by weight non-iodized sea salt, (viii) 0.01-0.10%) by weight of Baker's yeast; and (b) 1-20% by weight of a mixture of spores comprising Bacillus Subtillis spores, Bacillus Aglomerans spores and Bacillus
  • the instant invention further provies a bio-derived composition useful in controlling agricultural pests that infect palm trees comprising: (a) a product of fermenting a formulation comprising: (i) 1-10% by weight Green Peas, (ii) 1-10%> by weight Red beans, (iii) 1-10%> by weight Yellow corn, (iv) 1-10%> by weight Sorghum, (v) 5-10% by weight Yellow pine sawdust, (vi) 1 -10%> by weight Pine Oil, (vii) 0.1- 0.5%> by weight non-iodized sea salt, (viii) 0.01-0.10%) by weight of Baker's yeast; and (b) 1-20% by weight of a mixture of spores comprising Bacillus Subtillis spores, Bacillus Aglomerans spores and Bacillus Megaterium spores.
  • Palm bud rot caused by bacterial infections is often associated with cold damage and stress placed on the tree by exposure to cold temperatures.
  • the tree When a palm tree is exposed to cold damage, to prevent the possible onset of palm bud rot the tree should be treated with the product of Examples 1, 5, 6 or 8. .
  • the terminal bud Immediately after cold damage or damage from other bud rot causing agents such as from phytoptora palmivora the terminal bud should be sprayed, and then repeated every 10 to 14 days for a series of 4 treatments. It is best not to wait until palm bud rot symptoms emerge. Avoid pruning or removing damaged fronds, as this will place more stress on the tree and increase the potential for other bacterial or fungal infections to enter the tree.
  • palm trees When treating and preventing palm bud rot it is important to realize that palm trees have only one terminal bud from which all new growth emerges. Unlike most trees, such as maple and apple trees which have many points where new growth emerges, palms rely exclusively on their single terminal bud. If the terminal bud or heart becomes diseased or freezes during cold periods and dies, the tree will not be able to put out any new leaf growth and will die. That is why regular monitoring of the terminal bud and preventative care are vital to maintaining a healthy palm tree.
  • Palm Bud Rot It is always best to take steps to prevent the onset of Palm Bud Rot. Preventative steps should be taken if a palm tree has experienced cold damage or palm bud rot has been identified in the local area.
  • a preventative treatment spay the tree's temiinal bud with the products of the invention exemplified in Examples 5, 6 or 8 and repeat the treatment every 10 to 14 days, for 3 to 4 treatments, or as necessary.
  • a palm tree is already showing symptoms of palm bud rot, immediately treat the tree with the products of Examples 5, 6 or 8, focusing most attention towards the temiinal bud. Repeat the spraying every 7 days as necessary. Once a tree is infected with bud rot, it usually dies, but its chances of survival can increase the earlier it is treated with the formulations of the invention.
  • the products of Examples 5, 6 and 8 can also reverse bud rot, and also induce the natural resistance of the palm to the complex disease.
  • compositions of the invention are useful for treating the rice tarsonemid mite, Steneotarsonemus spinki which was first recorded in Baton Rouge, LA., USA in 1960. It has been recognized as an important rice pest in several Asian countries such as China, India, Taiwan, Korea, Philippines, and Thailand. The mite was detected in Cuba in 1997 causing severe yield losses, and subsequently was reported in the Dominican Republic, Haiti, Portugal, Costa Rica, and Panama causing 30 to 90% yield reduction. The mite was detected in Colombia in 2005 but in low populations with no significant yield reductions. Larvae and adults of S. spinki feed on the rice plant tissue causing browning of leaf sheaths and grain hulls.
  • the main damage is caused in association with the bacterial panicle blight pathogen (Burkholderia glumae) and detected during panicle emergence resulting in symptoms known as empty head or grain sterility.
  • the products of examples 1, 5, 6, 7 and 8 are particularly useful in combating this disease encountered in rice.
  • the rice tarsonemid mite is normally found associated with the fungus Sarocladium oryzae and recently was found in association with the bacterium Burkholderia glumae, the causal agent of bacterial panicle blight in Panama (2005 and 2006) and Colombia (2007), where the incidence of affected plants (grain sterility and discoloration) reached 100% causing yield losses above 80%.
  • the mite has recently (August 2007) been identified in Alvin, TX at the RiceTec research facility, the Texas A&M/ USDA ARS facility in Beaumont, TX, and at the winter nursery research facilities in Lajas, Puerto Rico. The locations where the mite has been found are under strict quarantine restriction.
  • the life cycle (egg to adult) of the mite takes 3-10 days depending on the temperature and relative humidity.
  • An adult female lays about 60-75 eggs inlO-15 days and 48 to 55 generations can be completed in a year.
  • the main host of the mite is the rice plant.
  • the optimal conditions for the growth and reproduction of the mite are 25-27C and relative humidity above 80%. Periods of sunny days and low rain favor mite development but low relative humidity and heavy rains increase mortality reducing the mite population.
  • the mite can be disseminated on seeds, by wind, water, insects, agricultural machinery, and survives on plant debris after harvesting. It can be detected on young plants when infested seed is planted or if neighboring fields were heavily infested.
  • the milky stage is preferred by the mite for feeding and its reproduction.
  • the mite is mainly detected in the inner part of the leaf sheath where high populations of nymphs and adults can be found. It is mainly found in the upper part of the sheath close to the leaf, but can also be found in the middle or lower part of the sheath. Small brown spots on the sheath are indications of mite presence.
  • the mite can also be found on the endosperm and the inner part of the hulls.
  • the products of the invention exemplified in examples 1, 5, 6, 7, and 8 are particularly effective in the control the rice tarsonemid mite avoiding broad dissemination of the bacterium B. glumae.
  • To prevent the introduction of the mite into a new crop it is necessary to destroy plant debris after harvesting infested fields, and disinfect the rice seed before planting.
  • planting dates should be modified to avoid favorable conditions for mite development during the most susceptible plant development stage of panicle formation and emergence. Differences in resistance to S. spinki have been observed among rice varieties, however results are not conclusive.
  • Cultural practices favoring a healthy crop development such as proper nitrogen fertilization, low seeding rates, destruction of plant residues, good land preparation and leveling and adequate water management help to reduce the problem.
  • the invention further provides bioremediation processes that are targeted and include the deliberate use of the biological, or respiratory, processes to degrade, consume, break down, transform, metabolize, and/or remove contaminants from a treatment zone that is associated with a contaminated region and may be performed both in situ and/or ex situ.
  • In situ bioremediation includes treating the contaminated material without removal from its current, existing, or natural location, while ex situ bioremediation includes removal of the contaminated material from its current, existing, or natural location for treatment at a different site.
  • bioremediation processes of the invention include the introduction of reactants, such as oxidants and/or nutrients, into the treatment zone to enhance, assist, augment, stimulate, and/or promote the growth of native microorganisms that are already present within the contaminated region and are termed bio-stimulation processes, while bioremediation processes that include the introduction of non- native microorganisms into the treatment zone, with or without the introduction of oxidants and/or nutrients, are termed bio-augmentation processes.
  • reactants such as oxidants and/or nutrients
  • the contaminated region must include a microbial population that is adapted to metabolize a contaminant, as well as an energy source, a carbon source, an electron acceptor (or oxidant), nutrients, and suitable environmental conditions.
  • the microbial population may include native microbes and/or may include specialized microbes that may be added to the treatment zone during a bio -augmentation process.
  • the contaminant is typically utilized by the microbial population as both the energy source and the carbon source, providing the mechanism by which the bioremediation processes may decrease a contaminant concentration within the treatment zone.
  • the bioremediation formulations of the invention may include both active and inactive components.
  • the active components may refer to components of the bioremediation formulation that are actively utilized during microbial respiration and/or components of the bioremediation formulation that actively participate in the delivery of the bioremediation formulation to the microorganisms, contaminant, treatment zone, and/or contaminated region.
  • inactive components of the bioremediation formulations of the invention are not actively utilized during microbial respiration and/or components of the bioremediation formulation that do not actively participate in the delivery of the bioremediation formulation to the microorganisms, contaminant, treatment zone, and/or contaminated region.
  • the invention further provides a method for treating an organic industrial waste to degrade the waste, which comprises: (a) providing a fermented product according to Example 1 and (b) adding the fermented product of Example 1 along with a diluent liquid to the industrial waste in a treatment plant, a lagoon, a tank or a soil, wherein the waste is degraded.
  • Organic waste that may be treated using the disclosed processes and composition include, but are not limited to agricultural waste, food waste, organic refuse, mill effluent, municipal waste, sewage, sludge, animal waste, and industrial waste.
  • Exemplary agricultural wastes include, but are not limited to, oil palm empty fruit bunch (EFB), palm decanter cake slurry, olive husk, corn cob, coffee bean husk, rice husk, rice straw, spent mushroom compost, palm foliage, palm trunk, palm kernel shells, palm fiber, farm effluent, slaughterhouse waste, flower cuttings, spent flower compost, wheat straw, fruit waste, vegetable waste, and the like.
  • EFB oil palm empty fruit bunch
  • palm decanter cake slurry include, but are not limited to, olive husk, corn cob, coffee bean husk, rice husk, rice straw, spent mushroom compost, palm foliage, palm trunk, palm kernel shells, palm fiber, farm effluent, slaughterhouse waste, flower cuttings, spent flower compost, wheat straw, fruit waste, vegetable waste, and the
  • Exemplary animal wastes include, but are not limited to, dead animals, animal feather, animal parts (such as animal intestines), and animal manure such as poultry manure, cow manure, goat manure, horse manure, sheep manure, and swine manure.
  • Mill effluent may be, for example, palm oil mill effluent (POME) and POME sludge.
  • the organic waste to be treated in the disclosed process may be selected based on criteria such as availability due to for example geographical or seasonal variability, cost, suitability, desired product and product properties, and the like. For example, in palm oil producing regions, about 8 million tons of empty fruit bunch (EFB) are generated annually, and hence provides an abundant source of organic waste that can be treated using the disclosed process to at least partly convert the EFB into useful organic fertilizer. Similarly, a typical food processing plant can generate between about 1.5 to about 2 tons of sludge per day while a poultry slaughterhouse can generate about 300 m 3 /day of wastewater, which result in abundant sources of organic waste for use in the disclosed process.
  • EFB empty fruit bunch
  • a single type of organic waste may be used in the disclosed process, or any combination of more than one type of organic waste may be used.
  • EFB may be used together with chicken manure
  • food waste may be used together with POME sludge.
  • Other exemplary combinations of organic wastes include, but are not limited to, a combination of chicken manure with dead chickens, a combination of chicken manure with chicken feathers, a combination of EFB with chicken manure, a combination of EFB with chicken manure and POME, and a combination of EFB and POME sludge.
  • Example 1 Once a product of the invention has been prepared (i.e., Example 1), it is ready to be applied to the organic waste. For example, if the organic wastes are contained in a lagoon, the product can be sprayed onto the surface of the lagoon or otherwise mixed with the contents of the lagoon for obtaining good coverage of the lagoon.
  • the amount of product that should be added to the lagoon is easily determined by a person of ordinary skill in the art without the need for undue experimentation. Addition of an appropriate amount of product will result in vigorous production of gas as the bacteria metabolize the wastes. Further, the bacteria will substantially control the odors associated with the lagoon within two to four weeks or less time.
  • Remedies for an upside down lagoon include (1) doing nothing, wherein the production of gas by the bacteria will gradually diminish and permit the solids to sink to the lagoon floor again, and (2) adding even more product to the lagoon, wherein the solids will be further metabolized by the bacteria and will sink to the floor of the lagoon.
  • the production of gas by the bacteria added to the lagoon results in mixing of the contents of the lagoon, which aids in mixing of the bacteria with the wastes. Since wastes will generally continue to be added to the lagoon, it is necessary to periodically add fresh product to the lagoon.
  • the fermentation products of the invention can also be used as ripeners and growth stimulants.
  • a pearticular composition that provides this effects includes by weight percent 10% Horse Tail Fern (Equisetaceae), 10.0% Plantain leaves, 2.0% White Onion, 2.0% Nettle leaves, 2.0%> Eucaliptus leaves and flowers, 2.0% Composition of Example 3, 71.49%) Potable Water and the composition also includes 0.10% Saccharomyces Cerevisiae.
  • CFU colony forming units
  • a product having the composition as shown in Table 2 below is made by the method shown below.
  • the product is filtered using a filter housing fitted with a 40 micron mesh filter and a recirculating pump is used in order to remove bio film that may be present in excess.
  • the product is filtered several times.
  • the product is then treated with ultraviolet light of a frequency of 240-280 nm to give an aseptic product.
  • step (10) Add to the mixture of step (10) 6.0 Kg of Bacillus Subtillis spores and mix by hand for 5 minutes.
  • Example 1 6 Liters of the product of Example 1 is further diluted with 30 liters of water and mixed manually and then allow mixing for about 15 minutes to start the latent phase.
  • Inorganic Composition as a fermentation additive
  • the inorganic component of the compositions is a mixture of naturally occurring minerals from the local rock formations.
  • Table 4 is a suitable composition.
  • the ingredients of the inorganic formulations are obtained in manual form from different regions in Colombia which are rich in these substances.
  • the phosphoric minerals have low phosphoric concentration and are obtained in the Colombian region of the Huila from the pasmont rocks and in the flat part of the soil, these minerals are very soft and are collected in manual form by the natives of the region.
  • the calcium minerals are also obtained from the region of the pasmont plains in the western mountains of the Colombian andes and also it is a soft material and is mined manually.
  • the silicieous minerals are obtained manually from the south region of Colombia in Valle of the Cauca (Jamundi's Municipality) bordering the Cauca.
  • the titanium and strontium containing minerals are also mined manually in Palmira's city in Colombia (Valle region) near the airport, and in the cities of Tulua and Buga. This ingredients are characterized as having Calcium, Magnesium, Manganese among others and about an additional 70 elements of very low concentration.
  • the minerals are ground or milled and passed through a mesh sieve of 40, 60 or 80 microns, to produce very fine powders similar to the talc.
  • Each of the inorganic raw materials is added to a container of suitable capacity and mixed manually achieve a homogeneous appearance.
  • leguminous components (Red Bean 0.2 Kg and Peas 0.2 Kg (Oats can be used instead of peas without processing) and mix them together and crush them to reduce the size of the grains.
  • step 6 Store the resulting mixture of step 6 in a cool and dry place, for eight days making sure there is enough space for the gases to escape from the process of fermentation.
  • the product is subjected to filtration using a filter provided with metallic mesh of 40 microns and then the liquid is recirculated with help of a pump in order to make sure the all the fine particles that could be present in the remaining biomass of the filtration could be removed.
  • the filetered product is further recirculated with the help of a diaphragm pump through a pipeline expossed to an electromagnetic field and then stored in a tank for application of UV light.
  • the product is the exposed to UV to create an aseptic product.
  • step 11 can be used as inoculum for further preparations if desired i.e., a small sample (500 ml) of the lot by storing it between 1.0 and 5.0 °C The sample is good for about 60 days.
  • the potable waters used in the manufacturing process must be collected and allowed to remain in an open environment for two hours before using to guarantee the elimination of the chlorine that could be present in it. Afterwards, the water is subjected to recirculation in the presence of an electromagnetic field and UV light.
  • the local water in Palmira, Valle, Colombia is ideal for this processes due to the fact it comes from natural high plateaus rivers or streams and has low pollution before being treated. This type of waters is used for all the formulations of the present invention.
  • step 6 Store the product of step 5 in a cool dry place for five days leaving enough space for ventilation so you can dispose of gases from, the fermentation process.
  • step 7 8. irradiate the product of step 7 using a UV lamp at a wavelength to provide an aseptic product.
  • the product is applied via. a foliar spray pump at 1 or 2 liters per acre.
  • the validity of the stored product is one year.
  • step (2) the amount of water used is 25.2 Kg and in step (5) Bacillus Aglomerans is used instead of
  • This product is also mixed with the product of example 4 in a 1 : 1 ratio, in this case Is 60 Kg with manual mixing for 5 minutes.
  • the product is applied via a foliar spray pump at 1 or 2 liters per acre.
  • the validity of the stored product is one year.
  • This product defined in Table 8 is useful to control and kill insects (insecticide).
  • This product is made using the same procedure as in the previous Example except in step (5) Bacillus Pseudomonas is used instead of Bacillus Aglomerans. This product is also mixed with the product of example 4 in a 1 : 1 ratio, in this ease is 60 Kg with manual mixing for 5 minutes.
  • the product is applied via a foliar spray pump at 1 or 2 liters per acre.
  • the validity of the stored product is one year.
  • This product defined in Table 9 is useful as a fungicide and bactericide.
  • step 7 Irradiate the product of step 7 using a IJ V lamp at a wavelength to provide an aseptic product.
  • This product defined in Table 10 is used as fertilizer on phytoplankton.
  • step ( 1) Mix all the blend of step ( 1) including cpmposition of Example 3 (Powder) with 12.0 Kg of Potable Water (This amount corresponds to twice the weight of the solids in the formulation).
  • step (8) Store the resulting mix of step (8) in a cooi dry place for five days leaving enough room for the gases from the fermentation process to escape.
  • step 7 Irradiate the product of step 7 using a IJ V lamp at a wavelength to provide an aseptic product.
  • This product defined in Table 11 is useful as a fertilizer.
  • step (5) Store the product of step (5) in a cool dry place for five days leaving enough space so the gases from the fermentation process can escape.
  • step 7 8. irradiate the product of step 7 using a UV lamp at a wavelength to provide an aseptic product.
  • the product is applied via foliar spray pump at 1 or 2 liters per acre.
  • the product has shelf life of one year.
  • the Guano component (2) is the material formed from coastal bird droppings. It is a very valuable material, due to its application in fertilizers and explosives. These characteristics are due to the guano high concentrations of phosphorus and nitrogen.
  • Chemically guano comprises ammonium nitrate together with uric acid, phosphoric acid, oxalic acid and carbonic addition salts and other impurities.
  • Table 12 The product defined in Table 12 is used as fertilizer, and it helps with the soil nitrogen fixation. Table 12
  • step 9 Irradiate the product of step 9 using a UV lamp at a wavelength to provide an aseptic product.
  • step (11) Add to the mixture of step (11), 9.0 Kg of the microorganism Azotobacter and mix by hand .for 5 minutes.
  • step 7 Add the mixture from the previous step to the rest of the product referenced in step (4) and mix in gentlv for about 5 minutes 8. Store product in a cool dry place, for eight days leaving some space for the gases from the fermentation process to escape.
  • step 9 Irradiate the product of step 9 using a UV lamp at a wavelength to provide an aseptic product.
  • step (11) Add to the mixture of step (11) 6.0 Kg of. Bacillus megaterium and mix by hand for 5 minutes.
  • This product defined in Table 14 is used as a degreaser and in composting processes, accelerates the degradation of organic matter and degrading organic compounds.
  • step 9 Irradiate the product of step 9 using a UV lamp at a wavelength to provide an aseptic product.
  • step (1 1 ) Add to the mixture of step (1 1 ) 6.0 Kg of Bacillus megaterium and mix by hand for 5 minutes.
  • This product defined in Table 15 is useful for remineralization of soils and crops, and increases soil biodynamic production.
  • This product defined in Table 16 is useful in sewage treatment systems, aerobic and facultative, oxidation ponds and soil contamination.
  • the product defined in Table 17 is used in aquacuiture activities and can be used in conjunction with the product of Example 9 to get best results.
  • the product defined in Table 18 is used in ethanol production and can be used in yc propagation.
  • the product is used in fermentation processes. It is recommended that the application be at a level of 20 ppm relative to the total fermentation mash.
  • the product of this example in Table 19 is used as a growth stimulant and ripener.
  • step 5 Filter the resulting product of step 4, using a filter housing fitted with 40 micron mesh and recirculate the filtrates using a pump in order to all the bio film which may be present in excess.
  • the yeast used is Saccharomyces cerevisiae. It is obtained commercially as baker's yeast. EXAMPLE 19
  • This product defined in Table 20 aids in the stabilization of stomach pH levels in order to prevent creation of stomach acids, which disrupt the digestive flow, preventing the erosion of the stomach mucus membrane and reducing the incidence of ulcers, gastritis, and reducing conditions which have been linked to stomach cancer. Reduces the number of the Helicobacter pilori bacteria in the intestinal track by trapping and elimination through the digestive tract and creating conditions for the increase of beneficial intestinal flora.
  • step ( 2) Place the mixture obtained in step ( 2) in a tray and spread it into a thin , even layer .
  • step (3) Expose the product of step (3) to UV treatment as in the previous examples. This step is done for safety as the product is characterized by having good stability and is not prone to contamination of any kind.
  • the composition of the present invention can be applied to plant parts as a dry material or as a wet formulation.
  • a dry material the composition can be applied directly to the plant parts to be protected, for example leaves.
  • the dry material can also be applied to the whole plant, fruits, and the like by dusting.
  • the wet formulation can be applied by spraying onto the plant parts to be protected, or the plant parts can be dipped in the wet formulation, for example by dipping them.
  • the water used for activation of the microorganisms present in the Example 1 product must be conditioned to a pH between 5 and 6, by using lemon juice was and monitored with a pH meter to adjust the pH to the required value .
  • Example 2 The microorganisms in the product of Example 1 were activated in a mixture with non- chlorinated water in a ratio of 1 :5 , i.e., one liter product of Example 1 with 5 liters unchlorinated water . Stir and let stand for 15 minutes.
  • the product was applied at the rate of a dose of 13 liters/acre. Prior to application a calibration was done. The second application was done at a dose of 7 liters/acre.
  • Sample populations per square meter were chosen randomly in the experimental acreage .
  • the first sampling took place five days after the first application.
  • the second sampling occurred four days after the second application.
  • Example 1 is very effective for controlling stable flies, Stomoxys calcitrans.
  • Ralstonia solanacearuni is a pathogen of worldwide distribution, infecting about 200 species of plants, including Musa and gingiberaceas and resposnsible for causing the disease Moko".
  • the organism is a Gram -negative bacterium. It has been studied by extensively by plant pathologists and used by molecular biologists as a model to explain the genetic mechanisms that govern pathogenicity in plants. The bacteria can survive in the soil, water and plant debris, plus it spreads rapidly and the established controls have proved inefficient in its eradication because of the versatility of the pathogen.
  • the product of Example 1 is particularly useful in reducing the aggressiveness of Ralstonia solanacearuni.
  • Moko on Heliconias caused by R. solanacearuni is a very versatile free-living organism.
  • the classification system of the bacteria makeup is governed by host range, pathogenicity, geographic distribution and genetic differences.
  • the bacteria can be revived even after a sleep state and persist in the infection of many plant species. In water the bacteria can survive in unfavorable conditions, achieves low latency can withstand temperatures critically and recover in a short time interval between 18° C and 25° C. Light is an important factor in the distribution of colonies, soils with R.
  • solanacearum can coexist with other species of bacteria, fungi and higher organisms and in some regions the presence of agricultural activity causes an imbalance in favor of harmful organisms such as Erwinia sp , Fusarium sp, Phytohpthora Rizotocnia sp and even nematodes.
  • the bacteria can spread from plant wounds, runoff, splash, infected seeds, insects and tools. For this disease nothing in the prior art is efficacious, although biological control and organic leachate extract in cultivated areas has provided interesting results in decreasing the incidence of ?. solanacearum.
  • Example 1 For application of the product of Example 1, we used two cans of 200 liters each. In a 200 liter container there were added 4 liters of Example 1 product to 20 liters of water, then allowed to stand for 15 minutes and then diluted further with 176 liters of water.
  • the other container is made the same way.
  • To the sprayer was added 10 liters of water and 10 liters of the product of Example.
  • the dosage per plant is 300 ml per plant.
  • Example 1 For the bio - activation the product of Example 1 is in a pre mix ratio of 1 liter of product with 5 liters of water, stir and let stand for 15 minutes. These products are applied without any mixing with others.
  • the field trial was conducted over a three month period on four lots of heliconias with different numbers of plants and different ages.
  • the products can be applied to the soil or the application can be made by foliar application.
  • the first two applications were made at an interval of two weeks, the other three applications were made at ten day intervals.
  • visual observations were made in the field to determine the behavior of plants such products. Samples from each batch of plants with or without symptoms of moko ⁇ Ralstonia solanacearum) were taken to the local Plant Health Diagnostic Laboratory to check for the presence of this bacterium in plants.
  • One batch had 198 plants, 1 months crop age, growing area 1485.37 m 2 . Of the 198 plants 185 were healthy plants, and 13 were diseased plants (7%). This batch was the most tolerant to this bacteria.
  • Palm oil producing palms that have bud rot are treated with the product of Examples 1, 5, 6 or 8.
  • Figure 1 shows an untreated palm spear having bud rot.
  • Figure 2 shows a treated palm spear which is free of bud rot after treatment.
  • a waste water pond containing waste from a pineapple processing plant is treated with the products of Example 1 (4.25 liters per day diluted with non-chlorinated water at a ration of 1 :5), Example 12 (1 liter per day diluted with non-chlorinated water at a ration of 1 :5), and Example 13 (2 liters per day diluted with non-chlorinated water at a ratio of 1 :5).
  • Example 18 Use of the Composition of Example 18 for Stimulating Growth/Ripening of Sugar Cane.
  • Example 18 The composition of Example 18 is applied to a sugar cane plantation at the rate of 1-2 liters per acre (or 13. 5 fluid ounces per acre) 3 months prior to harvesting.
  • the results of this example are summarized in Figures 4-7 and compared to glyphosate (control) which in the sugar cane industry is also used as a ripener.
  • Example 18 may be applied several times a year too.
  • stomach contents were detected.
  • the fish from the treated pond along with newly ingested pellets had lots of greenish color stuff similar to micro algae.
  • two of the fish only had a tiny presence of micro algae while tow of them had none.
  • Rice plantations that have been infected wit the rice tarsonemid mite and the bacterium B. glumae. are treated with the product of Examples 1, 5, 6, 7 or 8.
  • the rice plant shows significant improvement in not showing empty head or grain sterility

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Abstract

La présente invention porte sur une composition, qui est un pesticide naturel et organique, utilisée plus particulièrement pour limiter, lutter contre et traiter les micro-organismes pathogènes d'origine fongicide, virucide et bactéricide dans des produits agricoles tels que des plantes racines, des fruits et des légumes. Des exemples de ces micro-organismes provoquant des maladies sont Mycosphaerella fijensis qui provoque la maladie de Sigatoka noire chez le bananier nain, Ralstonia solanacearum qui provoque la maladie de Moko chez le bananier nain, Lasiodiplodia theobromae qui provoque la pourriture molle ou la pourriture du fruit dans les cultures et les fruits, Fusarium oxysporum qui provoque la maladie de Panama dans les fruits et les cultures, et de nombreux autres. La composition est un produit fermenté contenant des plantes tropicales, une source de carbone, une source de protéines (azote), et un agent excipient. Le produit fermenté peut aider à renforcer le système immunitaire des végétaux pour lutter contre des maladies pathogènes. Étant donné que tous les principaux constituants de la composition sont généralement considérés comme sans danger, ce pesticide naturel s'avère non toxique et sans danger pour les humains et les animaux, et inoffensif pour l'environnement.
PCT/US2015/015261 2014-02-10 2015-02-10 Compositions dérivées de produits biologiques destinées à être utilisées dans l'agriculture WO2015120472A1 (fr)

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MX2016010348A MX2016010348A (es) 2014-02-10 2015-02-10 Composiciones bioderivadas para su uso en agricultura.
BR112016018344A BR112016018344A2 (pt) 2014-02-10 2015-02-10 Composição, métodos para tratamento e prevenção de uma infestação de moscas de estábulos, para biorremediação de água, para tratamento de brotos podres em palmeiras, para controlar e reduzir a infestação de moscas de estábulo, para controlar a produção de odor em águas residuais e para proteger plantas de arroz contra doenças fúngicas, agente biológico, uso de uma composição, e, amadurecedor ou estimulante de crescimento bioderivado
AU2015213591A AU2015213591A1 (en) 2014-02-10 2015-02-10 Bio-derived compositions for use in agriculture
CA2939188A CA2939188A1 (fr) 2014-02-10 2015-02-10 Produits de fermentation microbienne des plantes pour le controle des phytopathogenies et l'assainissement des eaux usees
RU2016136350A RU2016136350A (ru) 2014-02-10 2015-02-10 Композиции биологического происхождения для применения в сельском хозяйстве
CN201580018762.7A CN106535642A (zh) 2014-02-10 2015-02-10 用于农业的生物衍生组合物
EP15745856.3A EP3104709A4 (fr) 2014-02-10 2015-02-10 Compositions dérivées de produits biologiques destinées à être utilisées dans l'agriculture
JP2016552333A JP2017512192A (ja) 2014-02-10 2015-02-10 本発明の農業分野での使用のためのバイオ由来組成物
IL247222A IL247222A0 (en) 2014-02-10 2016-08-10 Preparations of biological origin for agricultural use
PH12016501585A PH12016501585A1 (en) 2014-02-10 2016-08-10 Bio-derived compositions for use in agriculture

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US14/177,015 US10595535B2 (en) 2014-02-10 2014-02-10 Bio-derived compositions for use in agriculture
US14/177,015 2014-02-10
US14/177,199 US10595536B2 (en) 2014-02-10 2014-02-10 Bio-derived compositions
US14/177,203 US9770036B2 (en) 2014-02-10 2014-02-10 Bio-derived compositions for use in agricultural and environmental remediation
US14/177,203 2014-02-10
US14/177,199 2014-02-10

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RU2760673C1 (ru) * 2020-12-08 2021-11-29 Общество С Ограниченной Ответственностью "Илосан" Кранулированное удобрение, полученное с использованием плодородного субстрата
RU2761818C1 (ru) * 2020-12-08 2021-12-13 Общество С Ограниченной Ответственностью "Илосан" Способ получения основы препарата для обработки осадков сточных вод и/или отходов сельского хозяйства, навоза и помета, с использованием зерна риса
RU2761203C1 (ru) * 2020-12-08 2021-12-06 Общество С Ограниченной Ответственностью "Илосан" Применение препарата на основе растительного сырья для получения плодородного субстрата
RU2761202C1 (ru) * 2020-12-08 2021-12-06 Общество С Ограниченной Ответственностью "Илосан" Плодородный субстрат, полученный посредством обработки осадков сточных вод препаратом на основе растительного сырья
RU2761206C1 (ru) * 2020-12-08 2021-12-06 Общество С Ограниченной Ответственностью "Илосан" Способ получения основы препарата для обработки осадков сточных вод и/или отходов сельского хозяйства с использованием зерна пшеницы
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