WO2015020080A1 - バチルス属細菌を利用する農作物の栽培方法 - Google Patents
バチルス属細菌を利用する農作物の栽培方法 Download PDFInfo
- Publication number
- WO2015020080A1 WO2015020080A1 PCT/JP2014/070704 JP2014070704W WO2015020080A1 WO 2015020080 A1 WO2015020080 A1 WO 2015020080A1 JP 2014070704 W JP2014070704 W JP 2014070704W WO 2015020080 A1 WO2015020080 A1 WO 2015020080A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- strain
- bacillus subtilis
- crops
- cultivation
- mutant
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
Definitions
- the present invention relates to a method for cultivating agricultural crops using a bacterium belonging to the genus Bacillus, in particular, Bacillus subtilis (sometimes referred to as “Bacillus subtilis”).
- Bacillus subtilis sometimes referred to as “Bacillus subtilis”.
- Patent Documents 1 to 3 Chemical fertilizers and pesticides have been used extensively for the purpose of improving productivity of crops and controlling pests, but in recent years, interest in organic farming has increased. Coupled with such demands, the use of microorganisms in the agricultural field has attracted attention.
- endophytes that are microorganisms symbiotic with plants
- endophytes effective for controlling pests and endophytes effective for increasing plant growth and yield are known (Patent Documents 1 to 3).
- microorganisms such as Bacillus bacteria that are not endophytes but have a soil disease control ability that antagonizes plant pathogens in the soil or have a plant growth promoting action are also known (Patent Documents 4 to 9).
- microorganisms are specific strains. In order to select microorganisms that are highly useful in agriculture, it takes a lot of labor and time, and it is also necessary to determine whether the microorganisms can actually be used for agriculture.
- the present invention aims to provide a method for cultivating crops using Bacillus subtilis, thereby achieving growth promotion and yield increase of the crops.
- the present invention includes the following features.
- a Bacillus subtilis C-3102 strain (FERM BP-1096) having the ability to promote the growth and / or increase the yield of straw crops, or a mutant strain having the same ability
- a microbial material for agriculture characterized by containing as an active ingredient.
- Bacillus subtilis C-3102 strain or a mutant thereof further has an ability to promote flowering of crops or increase the flowering rate, according to any one of (1) to (3) Microbial material.
- a method for cultivating crops comprising cultivating crops using soil or a medium containing the microbial material according to any one of (1) to (6).
- the yield of crops is increased by shortening the internodes and improving the flowering rate, promoting the ripening of fruits, and improving workability without impairing the quality of the crops.
- the A remarkable effect was obtained especially for tomatoes.
- Internode shortening is considered to be effective in cucurbitaceous plants, which are crops that also have internodes.
- the improvement of flowering rate and the promotion of fruit maturation are expected to have the same effect on crops with fruit formation and maturation after flowering such as fruits and vegetables.
- This figure shows the result of measuring the internode length between the cotyledon and the first node at the stage where the tomato seedlings were planted in Example 1 and transferred to the drip irrigation system. The result is the average internode length of 262 strains in each section. Bacillus subtilis C-3102 strain (FERM BP-1096), 1% (w / v) added test area A, 0.1% (w / v) added test area B, and Bacillus subtilis C- A control group to which 3102 strain (FERM BP-1096) was not added was used. This figure is a graph showing the cumulative yield (kg) of tomatoes harvested from week 1 to week 10 in Example 2 in comparison with the control group for the test group.
- Bacillus subtilis strain C-3102 The bacterium used in the present invention is a Bacillus subtilis strain C-3102 having the ability to promote the growth of crops and increase the yield, or an equivalent thereof. It is a mutant strain having the ability (hereinafter also referred to as “C-3102 strain etc.”).
- the C-3102 strain has the ability to shorten the internode length of crops, the ability to promote flowering or increase the flowering rate of crops, the ability to promote the ripening of crops and / or It is further characterized by the ability to maintain the quality of the crop.
- internode length refers to the length from the cotyledon to the first node of the stem in the plant. The shorter the internode length, the more knots that bear fruit, such as tomatoes, leading to an increase in yield. In addition, the plant height is lowered, thereby improving workability.
- Bacillus subtilis C-3102 strain is registered by the applicant as an independent administrative corporation, National Institute of Advanced Industrial Science and Technology, Patent Biological Deposit Center (former Institute for Microbial Technology, Institute of Industrial Technology, Ministry of International Trade and Industry), East of Tsukuba City, Ibaraki Prefecture, Japan 305-8566 It was deposited as deposit number FERM BP-1096 on December 25, 1985 (1st, 1st, 1st, 6th). In the trust certificate, the microorganism is indicated as “Bacillus subtilis c-3102”, but “Bacillus subtilis C-3102 strain (FERM BP) described in this specification is used.
- the mutant strain of C-3102 strain can be obtained by artificial mutagenesis treatment, that is, by performing mutation using any appropriate mutagen for C-3102 strain.
- the mutagen includes a drug having a mutagen action, high energy ray irradiation, and the like.
- mutagens include ethyl methanesulfonate, N-methyl-N′-nitro-N-nitrosoguanidine, UV irradiation, heavy ion beam irradiation, X-ray irradiation, and gamma ray irradiation.
- Bacillus subtilis C-3102 strain can be prepared by culturing under appropriate conditions using a medium usually used for culturing microorganisms.
- the medium used for the culture contains a carbon source, a nitrogen source, inorganic salts, etc., and can be any of a natural medium, a synthetic medium, a liquid medium or a solid medium as long as it can efficiently culture Bacillus subtilis.
- Those skilled in the art can appropriately select a known medium suitable for the strain to be used. Lactose, glucose, sucrose, fructose, galactose, molasses, etc.
- organic sources such as peptone, casein hydrolyzate, whey protein hydrolyzate, and soy protein hydrolyzate can be used as the nitrogen source.
- Nitrogen-containing materials can be used.
- inorganic salts salts such as phosphate, sodium, potassium, magnesium, calcium, and trace elements can be used.
- amino acids, vitamins, surfactants and the like can be added to the medium.
- solid media suitable for culturing Bacillus subtilis include TS (tryptocase soy) agar and HI (heart infusion) agar.
- the culture of Bacillus subtilis is carried out at 20 ° C. to 50 ° C., preferably 30 ° C. to 45 ° C. under aerobic conditions.
- the temperature condition can be adjusted by a thermostatic bath, a mantle heater, a jacket, or the like.
- the culture format is stationary culture, shake culture, tank culture, or the like.
- the culture time can be 12 hours to 7 days, preferably 2 days to 3 days.
- the pH of the medium at the start of the culture is preferably maintained at 5-9, preferably 6-8.
- the culture solution may be used as it is, or the bacterial concentrate obtained by separating the bacterial culture solution by a separation means such as centrifugation, filtration separation, membrane separation or the like. It may be used in the form of Alternatively, a product obtained by drying (for example, freeze-drying) the C-3102 strain or the like may be used, or it may be immobilized on a carrier.
- the present invention relates to a Bacillus subtilis C-3102 strain (FERM BP-1096) having the ability to promote the growth of crops and increase the yield, or a mutant strain having the same ability. Is provided as an active ingredient, and an agricultural microbial material is provided.
- Microbial materials include, but are not limited to, bacterial cultures, bacterial concentrates, dried bacteria, immobilized bacteria, etc., such as strain C-3102.
- concentration of C-3102 strain and the like is not limited to the following, but the concentration in the soil is 1 ⁇ 10 3 to 1 ⁇ 10 8 pieces / g, preferably 1 ⁇ 10 5 to 1 ⁇ 10 8 pieces / g. In the case of g, more preferably about 1 ⁇ 10 7 to 1 ⁇ 10 8 pieces / g, any concentration can be used.
- Bacteria culture solution can be obtained by culturing in the medium and culture conditions conventionally used in Bacillus subtilis as exemplified above.
- the bacterial concentrate can be obtained, for example, by dehydrating the culture solution under reduced pressure at a temperature that does not kill the bacteria, or by concentrating the culture solution by filtration of the culture solution using a crossflow filter. .
- the dried bacterial product can be obtained, for example, by freeze-drying the culture solution.
- the bacterial immobilization product is a bacterial culture solution containing one or more carriers such as calcium carbonate, rice bran, activated carbon, diatomaceous earth, talc, zeolite, vermiculite, peat moss, perlite, bentonite, montmorite, preferably a porous carrier, It can be obtained by adsorbing to lysine and drying. At this time, the drying may be, for example, freeze drying or drying under reduced pressure. The dried product may be further ground to such an extent that it does not destroy the bacteria.
- carriers such as calcium carbonate, rice bran, activated carbon, diatomaceous earth, talc, zeolite, vermiculite, peat moss, perlite, bentonite, montmorite, preferably a porous carrier, It can be obtained by adsorbing to lysine and drying. At this time, the drying may be, for example, freeze drying or drying under reduced pressure. The dried product may be further ground to such an extent that it does
- the microbial material may be obtained by combining the C-3102 strain, which is an active ingredient, with an agriculturally acceptable arbitrary component and using it in various forms as the microbial material.
- Such forms include, but are not limited to, for example, powders, wettable powders, granules, liquids, suspensions, and the like.
- Optional components include, for example, diluents, excipients, binders, dispersants, extenders and the like.
- the concentration of microorganisms in the soil at the time of use is not limited to the following, but is 1 ⁇ 10 3 to 1 ⁇ 10 8 cells / g, preferably 1 ⁇ 10 5 to 1 ⁇ 10 8 cells / g, more preferably 1 It can be used as a microbial material so as to be about ⁇ 10 7 to 1 ⁇ 10 8 pieces / g.
- the concentration of the microorganism may be greater than 1 ⁇ 10 8 as described in the examples, for example 1 ⁇ 10 8 to 1.5 ⁇ 10 8 , or 1 ⁇ 10 8 to 2 ⁇ 10 8. Similar effects can be achieved.
- Crop cultivation method further provides a method for cultivating a crop, which includes cultivating a crop using soil or a medium containing the above-mentioned microbial material.
- cultivation both outdoor cultivation and indoor cultivation are conceivable. Examples include soil cultivation, house cultivation, hydroponics, and hydroponics.
- the microbial material of the present invention is suitable for house cultivation because it can shorten the internode length of the crop, reduce the height, and improve workability. Conventional techniques can be used for each cultivation method.
- Indoor cultivation includes house cultivation and plant factories. Indoor cultivation is literally indoor cultivation, and indoors may be equipped with irrigation equipment, temperature / humidity / light management equipment, nutrient solution replenishment equipment, and the like.
- an inorganic nutrient solution and / or an organic nutrient solution is added thereto as appropriate. Regularly replenish water and nutrient solution as the crop grows.
- Fertilizers that are included in or applied to soil, culture medium, etc. used in soil culture, hydroponics, hydroponics, etc. are usually used in slightly different compositions in soil culture, hydroponics and hydroponics Is done.
- nitrogen, phosphorus, and potassium are used as fertilizers, while in hydroponics and hydroponics, nitrogen is an essential element that plants absorb from the roots.
- a culture solution containing phosphorus, potassium, calcium, magnesium, sulfur, boron, iron, manganese, zinc, molybdenum or the like is used.
- Suitable medium components vary depending on the type of crop and vary depending on the variety, cultivation period, growth stage, temperature, light conditions, etc. ) Is generally used to adjust the concentration at the growth stage or cultivation stage.
- the cultivation method of the present invention promotes the growth of crops, increases the yield, shortens the internode length, promotes flowering, improves the flowering rate, matures the fruit and / or maintains the quality of the fruit.
- the maintenance of quality means that the weight, sugar content and acidity of the crop are not impaired as the yield increases.
- crops include, but are not limited to, eggplants, cucurbits, cruciferous plants, asteraceae plants, legumes, liliaceae, and celery plants.
- solanaceous plants include tomato, eggplant, bell pepper, shishito, chili pepper, potato, wolfberry, paprika, jalapeno, habanero and the like.
- cucurbitaceae plants examples include cucumbers, pumpkins, watermelons, and bitter melons.
- cruciferous plants examples include rape, turnip, chinsai, nozawana, mustard, Takana, Kobutana, mizuna, kohlraby, arugula, watercress, taasai, cauliflower, cabbage, kale, Chinese cabbage, komatsuna, Japanese radish, Japanese radish, broccoli, and medicinal cabbage. It is done.
- Examples of the Asteraceae plants include lettuce, sunny lettuce, garlic, chrysanthemum and the like.
- leguminous plants include soybean, azuki bean, groundnut, kidney bean, pea, vanilla bean, broad bean, cowpea, chickpea, mung bean, lentil, lentil and bambara bean.
- Examples of the lily family plants include onion, leek, rakkyo, garlic, leek, chives, lily, asparagus, and shallot.
- Physiological plants include carrots, honey bees, parsley, celery, celery, soup celery, charbell, fennel and the like.
- solanaceous plants such as tomatoes.
- Cucurbitaceae plants such as pumpkins and melons.
- the effects can be expected for basic, fruit vegetables, and fruits.
- Examples of watermelon, melon, strawberry, and fruit include apples, mandarin oranges, grapes, and peaches.
- Example 1 ⁇ Mixing of cells into soil> Bacillus subtilis C-3102 strain (FERM BP-1096) (1 ⁇ 10 10 cfu / g) to 1% (w / v) or 0.1% (w / v) with respect to seedling soil for tomato cultivation Added and mixed well. Bacillus subtilis C-3102 strain (FERM BP-1096), 1% (w / v) added test area A, 0.1% (w / v) added test area B and Bacillus subtilis C-3102 The test was carried out by providing three sections of the control group to which the strain (FERM BP-1096) was not added. The number of Bacilli subtilis C-3102 strain (FERM BP-1096) after mixing was as shown in Table 1.
- Bacillus subtilis C-3102 strain (1 ⁇ 10 10 cfu / g) was added to the seedling culture medium for tomato cultivation so as to be 0.1% (w / v) and mixed well. 2 of the test group to which 0.1% (w / v) of Bacillus subtilis strain C-3102 (FERM BP-1096) was added to the soil and the control group to which no Bacillus subtilis strain C-3102 (FERM BP-1096) was added A compartment was set up and the test was carried out. The number of Bacilli subtilis C-3102 strain (FERM BP-1096) after mixing was as shown in Table 3.
- Table 6 shows the results of measuring the harvest weight and number of tomatoes harvested during the cultivation period in the test group and the control group.
- FIG. 2 shows the results of accumulating the harvest weight every week after the start of harvesting. Throughout the harvest period, the harvest weight was 162.1 kg in the test plot, 142.1 kg in the control plot, and the number of harvests was 1643 in the test plot and 1462 in the control plot. This confirmed that the addition of Bacillus subtilis strain C-3102 (FERM BP-1096) increased the harvest weight per strain by 16.9% and the harvest number by 15.3%. Moreover, when the weight per tomato was compared, it was 98.7 g in the test group and 97.2 g in the control group, and it was confirmed that the yield was increased without losing the actual size.
- FERM BP-1096 Bacillus subtilis strain C-3102
- the tomatoes harvested in the test plot or the control plot in the early harvest (at the start of harvest), mid-term (1 month after the start of harvest) and late (2 months after the start of harvest) The sugar content and acidity were measured using 15 each. After removing the sticky tomato, it was put in a plastic bag with a chuck and crushed with a bag mixer for 30 seconds. Then, the crushed material was filtered out by exposure, and the sugar content and acidity were measured using the juice. Brix was measured using a refractometer for sugar content, and acidity was measured using an acid meter for acidity, and converted to citric acid concentration. The results are shown in Table 7.
- Example 3 ⁇ Mixing of cells into soil> Bacillus subtilis C-3102 strain (FERM BP-1096) (1 ⁇ 10 10 cfu / g) was added to the seedling culture medium for tomato cultivation so as to be 0.1% (w / v) and mixed well. 2 of the test group to which 0.1% (w / v) of Bacillus subtilis strain C-3102 (FERM BP-1096) was added to the soil and the control group to which no Bacillus subtilis strain C-3102 (FERM BP-1096) was added A compartment was set up and the test was carried out. The number of Bacilli subtilis C-3102 strain (FERM BP-1096) after mixing was as shown in Table 8.
- Tomato seeds were sown and cultivated for about 2 weeks. Then, using the soil produced above, it was cultivated in a pot (90 mm), and 246 strains were produced in the test group and 240 seedlings were produced in the control group.
- the present invention is useful in agriculture because Bacillus subtilis can be used for the cultivation of agricultural crops such as solanaceous plants to promote the growth of crops and increase the yield.
- Bacillus subtilis C-3102 strain is registered by the applicant as an independent administrative corporation, National Institute of Advanced Industrial Science and Technology, Patent Biological Deposit Center (former Institute for Microbial Technology, Institute of Industrial Technology, Ministry of International Trade and Industry), East of Tsukuba City, Ibaraki Prefecture, Japan 305-8566 It was deposited as deposit number FERM BP-1096 on December 25, 1985 (1st, 1st, 1st, 6th). At present, the deposited strain is stored and managed in the National Institute of Technology and Evaluation, the Patent Microorganisms Deposit Center (Postal Code 292-0818, Room 2-5-8 Kazusa Kamashi, Kisarazu City, Chiba Prefecture, Japan, Room 122).
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
- Cultivation Of Plants (AREA)
Abstract
Description
本発明において使用される細菌は、農作物の生育を促進し、かつ、収量を増加する能力を有するバチルス・ズブチルス(Bacillus subtilis)C-3102株、またはそれと同等の能力を有するその変異株(以下、「C-3102株等」と称することもある)である。
本発明は、農作物の生育を促進し、かつ、収量を増加する能力を有するバチルス・ズブチルス(Bacillus subtilis)C-3102株(FERM BP-1096)、またはそれと同等の能力を有するその変異株、を有効成分として含有することを特徴とする、農業用の微生物資材を提供する。
本発明はさらに、上記の微生物資材を含有する土壌または培地を用いて農作物を栽培することを含むことを特徴とする、農作物の栽培方法を提供する。
<土壌への菌体の混合>
バチルス・ズブチルス C-3102株(FERM BP-1096)(1×1010 cfu/g)をトマト栽培用育苗培土に対し、1%(w/v)または0.1%(w/v)となるように添加し、十分に混合した。バチルス・ズブチルスC-3102株(FERM BP-1096)を土壌に対し、1%(w/v)添加した試験区A、0.1%(w/v)添加した試験区Bおよびバチルス・ズブチルスC-3102株(FERM BP-1096)を添加しない対照区の3区画を設け、試験を実施した。混合後のバチルス・ズブチルスC-3102株(FERM BP-1096)の菌数は、表1に示したとおりであった。
トマトの種を各区画262粒ずつプラグポットに播種し、約2週間栽培した。その後、上記で作製した土壌を用いてポット(90 mm)にて栽培した。播種後、約1ヶ月で点滴潅水システムへ移行した。
上記の方法で苗を定植し、点滴潅水システムに移した段階で、子葉と第一節の間の節間長を測定した。その結果を表2および図1に示す。各区画262株ずつの節間長平均は、試験区Aで27.1 mm、試験区Bで30.0 mm、対照区では35.0 mmであり、バチルス・ズブチルス C-3102株(FERM BP-1096)の添加濃度に依存して節間が有意に短縮されることが確認された(P<0.01)。
<土壌への菌体の混合>
バチルス・ズブチルスC-3102株(FERM BP-1096)(1×1010 cfu/g)をトマト栽培用育苗培土に対し、0.1%(w/v)となるように添加し、十分に混合した。バチルス・ズブチルスC-3102株(FERM BP-1096)を土壌に対し、0.1%(w/v)添加した試験区およびバチルス・ズブチルスC-3102株(FERM BP-1096)を添加しない対照区の2区画を設け、試験を実施した。混合後のバチルス・ズブチルスC-3102株(FERM BP-1096)の菌数は、表3に示したとおりであった。
実施例1と同様の方法で点滴潅水システムに移し、水と液肥を供給しながら栽培を実施した。実が成熟した時点で収穫を開始し、72日間収穫した時点で栽培を終了した。栽培は全てハウス内で実施し、土壌へのバチルス・ズブチルスC-3102株(FERM BP-1096)の添加以外の条件は、各区画で一定にした。試験区は240株、対照区は246株を用いて栽培を行った。
収穫時期(一段目の実が成熟した時点)に、試験区および対照区において、トマトの背丈および花のついた段数の測定を行った。背丈を比較すると、対照区では収穫が困難な2 m以上の位置まで植物体が伸長していたのに対し、試験区では、2 m付近まで適度に伸長していた。また、各区画に対し、2 mの位置までに花のついた段数を測定すると、試験区では平均5.03段、対照区では平均4.87段となり、試験区においては収穫可能な高さ(2 m)までにより多くの段数が存在していた。このことから、バチルス・ズブチルスC-3102株(FERM BP-1096)を添加することにより、収穫時期においても節間短縮効果が維持されることが確認された。
上記の方法で栽培を実施し、収穫開始時期(播種後約5ヵ月)に試験区および対照区にて赤く色づき、収穫が可能な状態にある実の数を計測した。その結果を表5に示した。試験区においては、対照区の2倍以上の数の実が赤くなり、収穫が可能となっていた。このことより、バチルス・ズブチルスC-3102株(FERM BP-1096)の添加が、実の成熟を早期化することが確認された。
試験区および対照区において、栽培期間中に収穫したトマトの収穫重量、個数を測定した結果を表6に示した。また、収穫開始後、1週間ごとの収穫重量を累積した結果を図2に示した。収穫期間全体で、収穫重量は試験区では162.1 kg、対照区では142.1 kgであり、収穫個数は、試験区で1643個、対照区で1462個であった。このことから、バチルス・ズブチルスC-3102株(FERM BP-1096)の添加により、1株あたりの収穫重量は16.9%、収穫個数は15.3%増加することが確認された。また、トマト1個あたりの重量を比較したところ、試験区では98.7 g、対照区では97.2 gであり、実の大きさを損なうことなく、収量が増加していることが確認された。
<土壌への菌体の混合>
バチルス・ズブチルスC-3102株(FERM BP-1096)(1×1010 cfu/g)をトマト栽培用育苗培土に対し、0.1%(w/v)となるように添加し、十分に混合した。バチルス・ズブチルスC-3102株(FERM BP-1096)を土壌に対し、0.1%(w/v)添加した試験区およびバチルス・ズブチルスC-3102株(FERM BP-1096)を添加しない対照区の2区画を設け、試験を実施した。混合後のバチルス・ズブチルスC-3102株(FERM BP-1096)の菌数は、表8に示したとおりであった。
トマトの種を播種し、約2週間栽培した。その後、上記で作製した土壌を用いてポット(90 mm)にて栽培し、試験区は246株、対照区は240株の苗を作製した。
Claims (13)
- 農作物の生育を促進し、および/または、収量を増加する能力を有するバチルス・ズブチルス(Bacillus subtilis)C-3102株(FERM BP-1096)、またはそれと同等の能力を有するその変異株を有効成分として含有することを特徴とする、農業用の微生物資材。
- バチルス・ズブチルスC-3102株またはその変異株が、担体に固定化されていることを特徴とする、請求項1に記載の微生物資材。
- バチルス・ズブチルスC-3102株またはその変異株が、農作物の節間長を短縮する能力をさらに有することを特徴とする、請求項1または2に記載の微生物資材。
- バチルス・ズブチルスC-3102株またはその変異株が、農作物の開花を促進するまたは開花率を高める能力をさらに有することを特徴とする、請求項1~3のいずれか1項に記載の微生物資材。
- バチルス・ズブチルスC-3102株またはその変異株が、農作物の実の成熟を促進する能力をさらに有することを特徴とする、請求項1~4のいずれか1項に記載の微生物資材。
- バチルス・ズブチルスC-3102株またはその変異株が、農作物の実の品質を維持する能力をさらに有することを特徴とする、請求項1~5のいずれか1項に記載の微生物資材。
- 請求項1~6のいずれか1項に記載の微生物資材を含有する土壌または培地を用いて農作物を栽培することを含むことを特徴とする、農作物の栽培方法。
- 栽培が屋内栽培またはハウス栽培であることを特徴とする、請求項7に記載の方法。
- 栽培がポットを用いて行われることを特徴とする、請求項7または8に記載の方法。
- 栽培により農作物の生育の促進、収量の増加、節間長の短縮、開花の促進、開花率の向上、実の成熟の促進または実の品質の維持が生じることを特徴とする、請求項7~9のいずれか1項に記載の方法。
- 農作物が、ナス科植物およびウリ科植物から選択される作物である、請求項7~10のいずれか1項に記載の方法。
- 農作物がトマトである、請求項7~11のいずれか1項に記載の方法。
- バチルス・ズブチルスC-3102株(FERM BP-1096)またはその変異株の農業用微生物資材への使用。
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015530919A JP6434409B2 (ja) | 2013-08-09 | 2014-08-06 | バチルス属細菌を利用する農作物の栽培方法 |
EP14834688.5A EP3031790B1 (en) | 2013-08-09 | 2014-08-06 | Method for cultivating crops using bacterium belongings to genus bacillus |
US14/910,792 US10899677B2 (en) | 2013-08-09 | 2014-08-06 | Method for cultivating crops using bacterium belonging to genus Bacillus |
CN201480042927.XA CN105431397B (zh) | 2013-08-09 | 2014-08-06 | 使用芽孢杆菌属细菌栽培作物的方法 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2013166933 | 2013-08-09 | ||
JP2013-166933 | 2013-08-09 | ||
JP2013-256473 | 2013-12-11 | ||
JP2013256473 | 2013-12-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2015020080A1 true WO2015020080A1 (ja) | 2015-02-12 |
Family
ID=52461411
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2014/070704 WO2015020080A1 (ja) | 2013-08-09 | 2014-08-06 | バチルス属細菌を利用する農作物の栽培方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US10899677B2 (ja) |
EP (1) | EP3031790B1 (ja) |
JP (1) | JP6434409B2 (ja) |
CN (1) | CN105431397B (ja) |
TW (1) | TWI660672B (ja) |
WO (1) | WO2015020080A1 (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10681877B1 (en) * | 2017-08-02 | 2020-06-16 | Robert Goodwin | Plant growing system |
CN109836190A (zh) * | 2019-02-18 | 2019-06-04 | 曹翠玲 | 一种利用生防细菌制备生防菌肥及消除甜瓜连茬障碍的方法 |
KR102125338B1 (ko) * | 2019-11-14 | 2020-06-22 | 우신 영농조합법인 | 율피 발효액 및 이를 이용한 토마토의 재배 방법 |
CN114437980B (zh) * | 2022-02-09 | 2023-11-24 | 山东农业大学 | 一种微生物菌剂及其应用 |
CN115287214B (zh) * | 2022-05-25 | 2023-04-14 | 甘肃省农业科学院植物保护研究所 | 一种复合微生物、复合微生物菌剂和应用 |
Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63209580A (ja) * | 1987-02-25 | 1988-08-31 | Karupisu Shokuhin Kogyo Kk | バチルス・ズブチリスc−3102 |
JPH10276579A (ja) | 1997-04-09 | 1998-10-20 | Ee H C:Kk | バチルス属微生物を用いた植物の生長促進剤および生長促進方法 |
WO2000042169A1 (fr) | 1999-01-14 | 2000-07-20 | Gold Kosan Co. Ltd. | Nouveau micro-organisme et utilisation de celui-ci |
JP2004215567A (ja) | 2003-01-14 | 2004-08-05 | Kansai:Kk | バチルス属に属する微生物及びそれを用いる防除剤 |
WO2004067197A1 (ja) * | 2003-01-31 | 2004-08-12 | Calpis Co., Ltd. | 有機廃棄物処理方法、有機廃棄物処理剤およびそれに用いる微生物 |
JP2006016386A (ja) | 2004-05-31 | 2006-01-19 | Showa Denko Kk | 微生物含有組成物 |
JP2009232721A (ja) | 2008-03-26 | 2009-10-15 | Univ Of Miyazaki | エンテロバクター属細菌を用いた植物栽培方法 |
JP2010530350A (ja) * | 2007-06-20 | 2010-09-09 | ウルトラ バイオテック リミテッド | 微生物製剤および植物の成長促進にそれを使用する方法 |
JP2011051902A (ja) | 2009-08-31 | 2011-03-17 | Tottori Univ | 斑点米カメムシ忌避物質 |
JP2012526073A (ja) | 2009-05-06 | 2012-10-25 | ビーエーエスエフ ソシエタス・ヨーロピア | 病原体圧の本質的に非存在下で農作物の活力および/または作物収穫量を増加させる方法 |
JP2013042695A (ja) | 2011-08-24 | 2013-03-04 | Ibaraki Univ | 根部エンドファイト(DSE)Phialocephalafortiniiを利用したアスパラガス苗の生産方法 |
WO2013035804A1 (ja) * | 2011-09-08 | 2013-03-14 | カルピス株式会社 | 芽胞形成菌の発芽誘起方法 |
JP2013060378A (ja) * | 2011-09-12 | 2013-04-04 | Calpis Co Ltd | 有害菌の殺菌方法 |
JP2013066467A (ja) | 2011-09-22 | 2013-04-18 | Republic Of Korea (Rural Development Administration) | バチルスバリスモルティスbs07m菌株、微生物製剤、及び作物育成方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4551164A (en) * | 1983-10-04 | 1985-11-05 | Bio-Organics, Inc. | Microbial plant growth promoter |
CN85108913A (zh) | 1985-11-04 | 1987-05-20 | 生物有机体公司 | 微生物的植物生长促进剂及产量增加剂 |
DE4237146A1 (de) * | 1992-11-04 | 1994-05-05 | Dieter Dipl Ing Baier | Pilliertes Saatgut |
CN1073142C (zh) | 1994-04-21 | 2001-10-17 | 长濑生化学工业株式会社 | 农业用细菌制剂 |
US5733355A (en) * | 1994-09-29 | 1998-03-31 | Susumu Hibino | Bacterial Preparation for agricultural use |
US20030228679A1 (en) | 2002-03-27 | 2003-12-11 | Smith Donald L. | Compositions and methods for increasing plant growth by inoculation with bacillus strains |
JP2006013686A (ja) * | 2004-06-23 | 2006-01-12 | Matsushita Electric Ind Co Ltd | 陰極線管装置および画像表示装置並びに画像表示装置の駆動方法 |
US20060008512A1 (en) * | 2004-07-07 | 2006-01-12 | Hooge Danny M | Composition and methods for improved animal performance |
TW201136528A (en) | 2010-03-17 | 2011-11-01 | Calpis Co Ltd | Agent for improving feed utilization efficiency of ruminant |
CN102816725B (zh) * | 2012-09-07 | 2013-11-06 | 江苏省农业科学院 | 一株枯草芽孢杆菌及其应用 |
CN102910988B (zh) * | 2012-11-18 | 2014-08-20 | 北京市波普克生态科技有限公司 | 一种防治植物病虫害的复合微生物液体菌肥及其制备方法 |
WO2015037661A1 (ja) * | 2013-09-16 | 2015-03-19 | カルピス株式会社 | 微生物による家畜敷料の殺菌方法 |
-
2014
- 2014-08-06 JP JP2015530919A patent/JP6434409B2/ja active Active
- 2014-08-06 CN CN201480042927.XA patent/CN105431397B/zh active Active
- 2014-08-06 EP EP14834688.5A patent/EP3031790B1/en active Active
- 2014-08-06 WO PCT/JP2014/070704 patent/WO2015020080A1/ja active Application Filing
- 2014-08-06 US US14/910,792 patent/US10899677B2/en active Active
- 2014-08-07 TW TW103127127A patent/TWI660672B/zh active
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63209580A (ja) * | 1987-02-25 | 1988-08-31 | Karupisu Shokuhin Kogyo Kk | バチルス・ズブチリスc−3102 |
JPH10276579A (ja) | 1997-04-09 | 1998-10-20 | Ee H C:Kk | バチルス属微生物を用いた植物の生長促進剤および生長促進方法 |
WO2000042169A1 (fr) | 1999-01-14 | 2000-07-20 | Gold Kosan Co. Ltd. | Nouveau micro-organisme et utilisation de celui-ci |
JP2004215567A (ja) | 2003-01-14 | 2004-08-05 | Kansai:Kk | バチルス属に属する微生物及びそれを用いる防除剤 |
WO2004067197A1 (ja) * | 2003-01-31 | 2004-08-12 | Calpis Co., Ltd. | 有機廃棄物処理方法、有機廃棄物処理剤およびそれに用いる微生物 |
JP2006016386A (ja) | 2004-05-31 | 2006-01-19 | Showa Denko Kk | 微生物含有組成物 |
JP2010530350A (ja) * | 2007-06-20 | 2010-09-09 | ウルトラ バイオテック リミテッド | 微生物製剤および植物の成長促進にそれを使用する方法 |
JP2009232721A (ja) | 2008-03-26 | 2009-10-15 | Univ Of Miyazaki | エンテロバクター属細菌を用いた植物栽培方法 |
JP2012526073A (ja) | 2009-05-06 | 2012-10-25 | ビーエーエスエフ ソシエタス・ヨーロピア | 病原体圧の本質的に非存在下で農作物の活力および/または作物収穫量を増加させる方法 |
JP2011051902A (ja) | 2009-08-31 | 2011-03-17 | Tottori Univ | 斑点米カメムシ忌避物質 |
JP2013042695A (ja) | 2011-08-24 | 2013-03-04 | Ibaraki Univ | 根部エンドファイト(DSE)Phialocephalafortiniiを利用したアスパラガス苗の生産方法 |
WO2013035804A1 (ja) * | 2011-09-08 | 2013-03-14 | カルピス株式会社 | 芽胞形成菌の発芽誘起方法 |
JP2013060378A (ja) * | 2011-09-12 | 2013-04-04 | Calpis Co Ltd | 有害菌の殺菌方法 |
JP2013066467A (ja) | 2011-09-22 | 2013-04-18 | Republic Of Korea (Rural Development Administration) | バチルスバリスモルティスbs07m菌株、微生物製剤、及び作物育成方法 |
Non-Patent Citations (1)
Title |
---|
See also references of EP3031790A4 |
Also Published As
Publication number | Publication date |
---|---|
US20160185676A1 (en) | 2016-06-30 |
TW201536181A (zh) | 2015-10-01 |
CN105431397A (zh) | 2016-03-23 |
JP6434409B2 (ja) | 2018-12-05 |
JPWO2015020080A1 (ja) | 2017-03-02 |
EP3031790B1 (en) | 2019-02-13 |
EP3031790A1 (en) | 2016-06-15 |
US10899677B2 (en) | 2021-01-26 |
CN105431397B (zh) | 2020-08-25 |
EP3031790A4 (en) | 2017-04-12 |
TWI660672B (zh) | 2019-06-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
RU2628411C2 (ru) | Микробные инокулянты и содержащие их композиции удобрений | |
JP5714603B2 (ja) | 植物の出芽および生長を増強するためのシュードモナス・アゾトフォルマンス(pseudomonasazotoformans)種の新規蛍光シュードモナス菌 | |
JP6434409B2 (ja) | バチルス属細菌を利用する農作物の栽培方法 | |
KR20140023306A (ko) | 식물의 육성 방법 및 그것에 사용하는 조성물 | |
WO2010109436A1 (en) | Microbial formulation for widespread uesd in agricultural practices | |
KR102470864B1 (ko) | 양파의 생육 촉진 효과 및 가뭄 내성 증진 효과를 갖는 바실러스 알티투디니스(Bacillus altitudinis) H5-9 균주 및 이의 용도 | |
JPWO2016021204A1 (ja) | 植物生育促進剤及び植物生育促進方法 | |
Hata et al. | Production of Soybean Plants for Hydroponic Cultivation from Seedling Cuttings in a Medium Containing Inoculum Depending on Various Concentrations of Nutrient Solution and Different Nitrogen Sources | |
KR101934341B1 (ko) | 신균주 총명버섯의 재배방법 | |
Gicharu et al. | Variation in nitrogen fixation among three Bush Bean cultivars grown in Kenya when inoculated with three Rhizobia Strains | |
JP2014080398A (ja) | 植物成長調整剤 | |
KR20110110769A (ko) | 식물 병해 방제용 조성물, 식물 병해 방제 방법, 및 신규 미생물 | |
RU2736340C9 (ru) | Средство для стимуляции роста сельскохозяйственных культур | |
JPH0823963A (ja) | 植物の栽培方法 | |
JP3905553B2 (ja) | 新規好熱菌及びこれを含有する土壌配合材 | |
EP3462879B1 (fr) | Méthode pour améliorer le développement des plantes | |
KR930009509B1 (ko) | 두과작물 증수를 위한 근류균 접종제 | |
WO2023095759A1 (ja) | 植物の培土、培土を含む栽培セット、培土を用いた栽培方法、及び、培土付き植物の苗 | |
US20240018063A1 (en) | Composition for bio stimulation of plants | |
RU2826882C1 (ru) | Штамм эндомикоризного гриба Rhizophagus intraradices и микробиологическое удобрение на его основе | |
KR102239389B1 (ko) | 고추 가뭄 스트레스 저감 미생물 바실러스 부타놀리보란스 kj40 및 이의 용도 | |
JP2011168532A (ja) | 植物芽発酵液と枯草菌からなる植物栽培用組成物、および植物栽培方法 | |
JP2022183435A (ja) | 植物成長調整剤及びその製造方法 | |
JPH107483A (ja) | 植物の栽培促進剤およびそれを用いた植物の栽培促進方法 | |
Maghirang et al. | Organic eggplant production |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 201480042927.X Country of ref document: CN |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 14834688 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2015530919 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 14910792 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2014834688 Country of ref document: EP |