WO2015012391A1 - 試薬供給装置 - Google Patents
試薬供給装置 Download PDFInfo
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- WO2015012391A1 WO2015012391A1 PCT/JP2014/069704 JP2014069704W WO2015012391A1 WO 2015012391 A1 WO2015012391 A1 WO 2015012391A1 JP 2014069704 W JP2014069704 W JP 2014069704W WO 2015012391 A1 WO2015012391 A1 WO 2015012391A1
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- reagent
- container
- nozzle
- liquid
- reagent supply
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8822—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1079—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices with means for piercing stoppers or septums
Definitions
- the present invention relates to a method for supplying a reagent to a sample analyzer, an apparatus therefor, and a sample analysis method using the reagent supply method.
- Sample separation and analysis methods such as liquid chromatography are widely used for analysis and inspection in fields such as organic chemistry, biochemistry, and medicine. These separation and analysis methods use various reagents such as eluents for separating samples, washing solutions for washing columns and reagent channels, diluting solutions for diluting samples, washing diluting solutions for both washing and dilution. ing. Conventionally, these reagents have been provided in a state of being packed in glass or resin bottles. However, when a reagent is packed in a glass or resin bottle, there is a problem that if the reagent is consumed and voids are formed in the bottle, the reagent is concentrated due to evaporation or condensation of volatile components.
- Patent Document 1 as a container whose volume can be shrunk with a decrease in the reagent and which is excellent in the storage stability of the reagent, a flexible product obtained by processing a laminate of a resin layer and an aluminum foil layer into a bag shape.
- a sex reagent bag is disclosed.
- Non-Patent Document 1 the above-described container-packing reagent is sucked up by a suction nozzle or the like that is lowered from the mouth at the top of the container and supplied to the analyzer.
- a suction nozzle or the like that is lowered from the mouth at the top of the container and supplied to the analyzer.
- the remaining amount of the reagent in the container decreases, it becomes difficult to suck the reagent from the suction nozzle, or bubbles due to air sucked together with the reagent are generated, so that an appropriate amount of reagent cannot be supplied. As a result, accurate analysis cannot be performed.
- Patent Document 3 discloses that the reagent can be used up without sucking air even if the remaining amount of the reagent is small, and has a slope or a depression at the bottom, and a reagent suction nozzle at the lowest part of the slope or the depression.
- a reagent container in which the tip of is arranged is disclosed.
- this reagent container also has a long suction nozzle, there has been a problem that bubbles due to reduced pressure in the nozzle are likely to be generated during reagent suction.
- the present invention provides a reagent supply method that allows the reagent in the reagent container to be used up without leaving as much as possible, and that allows the reagent to be supplied to the analyzer without mixing bubbles even when the remaining amount of the reagent becomes small. It is another object of the present invention to provide an apparatus therefor.
- the present inventors flow the reagent downward from a reagent container having a reagent supply port disposed on the lower side of the container body, and send the flowed reagent to the analyzer.
- the reagent in the reagent container can be used up without leaving as much as possible, and that an accurate amount of reagent can be supplied to the analyzer without introducing bubbles even when the remaining amount of the reagent becomes small.
- the present inventors can provide a highly durable reagent container free from liquid leakage and contamination of reagents in the container even when used for a long period of time by manufacturing the reagent supply port with a material having a predetermined hardness. I found out.
- the present invention is a reagent supply device, At least one reagent container having a container main body for storing a liquid reagent and a reagent supply port provided in the container main body, and each of the at least one reagent container is provided with a reagent supply port of the container main body.
- the reagent supply port has at least one nozzle penetration part, Each of the at least one nozzle penetration is made of a material having a Shore hardness of A5 ° to A90 °.
- a reagent supply device is provided.
- the present invention also provides a sample analyzer comprising the reagent supply device and a sample analysis unit.
- the present invention also provides a reagent supply method to the sample analysis unit, Installing at least one reagent container;
- each of the at least one reagent container has a container main body for storing a liquid reagent and a reagent supply port provided on the lower side of the container main body, and the reagent supply port has at least one nozzle penetration part.
- each of the at least one nozzle penetration is made of a material having a Shore hardness of A5 ° to A90 °, and,
- a method is provided which comprises causing the liquid reagent to flow downward from a nozzle inserted into the reagent container through the nozzle penetrating portion and then feeding the liquid reagent to a sample analysis unit.
- the present invention also provides a sample analysis method, Installing at least one reagent container;
- each of the at least one reagent container has a container main body for storing a liquid reagent and a reagent supply port provided on the lower side of the container main body, and the reagent supply port has at least one nozzle penetration part.
- each of the at least one nozzle penetration is made of a material having a Shore hardness of A5 ° to A90 °, The liquid reagent is allowed to flow downward from the nozzle inserted into the reagent container through the nozzle penetrating portion, and then fed to the sample analysis unit, and the sample is processed or analyzed by the fed liquid reagent.
- a method comprising:
- the reagent supply apparatus of the present invention since the reagent supply port is arranged on the lower side of the container body, the liquid reagent can flow out from the supply port toward the lower side of the container. There is no need to suck up. Therefore, according to the reagent supply apparatus of the present invention, the reagent in the container can be used up without remaining, and even if the remaining amount of the reagent becomes small, bubbles are not mixed into the reagent.
- “use up all reagents without leaving” means that the conventional reagent supply method is compared with the present invention, and the present invention includes a state in which the remaining amount of the reagent is less at the end of use of the reagent.
- the reagent supply apparatus of the present invention since the reagent is supplied through the nozzle inside the sealed reagent container, the contamination of the reagent accompanying the opening of the container and the contact between the user and the reagent are prevented. Can do.
- the nozzle penetrating portion is made of a material having a large elastic limit, even if it is used for a long time with the nozzle penetrating, there is no leakage of the reagent from the penetrating portion, and it can withstand long-term use.
- the present invention provides an accurate and economical means for supplying the appropriate amount of reagent to the analyzer.
- the schematic diagram of a reagent container The schematic diagram of the cross section of a reagent supply port. The schematic diagram of the cross section of the housing which accommodated the reagent container. The schematic diagram which shows the supporting member which supports a reagent container. The schematic diagram of the reagent supply apparatus connected with the sample analysis unit. The schematic diagram of a blood sample analyzer.
- the reagent supply apparatus of the present invention can be used to supply reagents to various sample analysis units such as liquid chromatographs and blood test equipment.
- the number and types of reagents that can be supplied by the reagent supply device of the present invention are not particularly limited as long as they are liquid reagents, and the reagent supply device of the present invention is not limited to the types and number of reagents necessary for the desired analysis. In response, one, two, three or more types of liquid reagents can be supplied.
- the reagent supplied by the reagent supply apparatus of the present invention include a reagent for liquid chromatography, a reagent for analyzing a biological sample such as blood, and a reagent for clinical examination.
- the reagent supply apparatus of the present invention includes one or a plurality of reagent containers.
- Each of the reagent containers contains the same or different types of liquid reagents, and the number of reagent containers and the types of reagents to be stored should be appropriately selected according to the analysis method in which the reagents are used. Can do.
- Each of the reagent containers has a container main body for storing a liquid reagent and a reagent supply port provided on the lower side of the container main body.
- the material of the container body of the reagent container is not particularly limited, and examples thereof include glass, metal, resin, and a laminate thereof.
- a light-resistant material such as metal, stained glass, and stained resin.
- the container body is a flexible container made of a flexible material.
- the container is deflated as the liquid reagent in the container is consumed. Therefore, even when the remaining amount of the reagent is reduced, it is possible to prevent a large gap from being formed inside the container. As a result, the concentration of the reagent due to evaporation or condensation of the volatile component from the liquid reagent inside the container can be prevented.
- the flexible container examples include a resin bag and a bag made of a laminated body such as a resin layer and a metal layer.
- a resin bag and a bag made of a laminated body such as a resin layer and a metal layer.
- a polyolefin layer, a polyester resin layer or a polyester resin layer on which an inorganic compound is deposited, and an aluminum foil layer are laminated to obtain a laminate.
- the port is made of a rigid material and is connected to the reagent supply port by screwing, fitting, meshing, or the like.
- each component layer such as the polyolefin layer is increased within a range that does not adversely affect the quality of the reagent to be stored, for example, polyamide resin, polyester resin, etc.
- a reinforcing material can be used for the material.
- the flexible container may have a self-supporting shape. In order to improve operability, a handle or the like may be provided as necessary.
- the reagent supply apparatus of the present invention may include a housing that houses the one or more reagent containers.
- the housing has a role of protecting the reagent container, and a guide for arranging each reagent container at an appropriate position with respect to the sample analysis unit so that an appropriate reagent is supplied to the analysis unit.
- the role of can be combined.
- the housing is a reagent cartridge set in an analyzer having a sample analysis unit.
- the shape (appearance) can be either symmetric or asymmetric. When the asymmetric shape is adopted, the direction of the cartridge set in the analyzer can be defined, so that a plurality of reagent containers in the cartridge can be connected to the analyzer in proper alignment.
- the arrangement of the reagent containers in the housing may be uniform or unequal.
- the reagent container is configured to be stored in the housing in an uneven arrangement, and the analyzer having the sample analysis unit is provided with a support member for supporting the reagent container and the housing in a corresponding uneven arrangement.
- the housing can be set in accordance with the shape of the support member, and the reagent containers can be connected to the analyzer in proper alignment.
- housing material examples include polypropylene, polyethylene, nylon, polyethylene terephthalate, polyacetal, polyamide, polybutylene terephthalate, ABS resin, polystyrene, AS resin, and polymethyl methacrylate resin. Good.
- the reagent supply device of the present invention also includes a support member that supports the reagent container.
- the support member may constitute one or more members that individually support the one or more reagent containers, or one member that collectively supports the one or more reagent containers. There may be.
- the support member may support the reagent container by supporting a housing that houses the reagent container described above.
- each reagent container is arranged by the support member so that the reagent supply port is located below the reagent container main body. Therefore, in the reagent supply apparatus of the present invention, the liquid reagent inside the reagent container flows downward from the lower side of the container.
- the reagent supply apparatus of the present invention when a flexible container is used as the reagent container, it is not necessary to evacuate the reagent container before use, which is necessary in the conventional reagent container installation procedure.
- the reagent in the container can be used up to the end.
- the reagent supply port of the reagent container is not opened when not in use, and has one or a plurality of nozzle penetrating portions that allow the nozzles to pass therethrough.
- the reagent supply port is a plug attached to the reagent container, and the plug has one or a plurality of nozzle penetrating portions.
- One end of the nozzle penetrating portion is an outward surface facing the outside of the container, and the opposite end is an inward surface facing the inside of the container.
- the nozzle inserted in the outward surface can penetrate to the inward surface.
- the nozzle passes through the plug for sealing the reagent supply port, and the liquid reagent in the container is supplied to the outside through the nozzle.
- the reagent is supplied by penetrating the nozzle into the sealed reagent container without opening the reagent container. Therefore, the contamination of the reagent accompanying the opening of the container and the user Can be prevented from contacting with the reagent.
- the shape of the nozzle penetrating portion is not particularly limited as long as it does not cause liquid leakage from the penetrating portion even if left for a long time with the nozzle penetrating, but the pressure applied to the nozzle such as a cylindrical shape is not limited. A shape that is uniform is preferred. Further, the nozzle penetrating portion is made of a material that does not leak and has little elution into the reagent and does not adversely affect the quality of the reagent when in contact with the stored liquid reagent. desirable.
- the nozzle penetrating portion is made of a material that does not leak liquid from the penetrating portion even when left for a long period of time, for example, three months or more with the nozzle penetrating, for example, a material having a Shore hardness of A5 ° to A90 °. It is preferable to be configured.
- the Shore hardness is more preferably A60 ° or less, and further preferably A50 ° or less.
- As a lower limit of Shore hardness it is good to make A5 degree into a lower limit from the point of stickiness suppression.
- Synthetic rubbers (isoprene rubber, isoprene-isobutylene copolymer, butyl rubber, butadiene rubber, ethylene-propylene copolymer, ethylene-propylene-third component copolymer, urethane rubber, silicone rubber, etc.) ), Natural rubber, elastomer and the like, preferably olefin elastomer, styrene elastomer and the like.
- the material may be mixed with a crosslinking accelerator. Moreover, you may mix
- the shore hardness can be measured in accordance with a shore hardness test defined in Japanese Industrial Standard JIS Z 2246.
- the plug may be a nozzle penetrating part that can penetrate the nozzle as a whole, but preferably has at least a peripheral part of the plug body made of a rigid material that the nozzle cannot penetrate.
- the material of the plug body include those having a Rockwell hardness R80 to 110. The Rockwell hardness can be measured according to the Rockwell hardness test specified in Japanese Industrial Standard JIS G0202.
- the material constituting the plug body is preferably a material that is excellent in adhesiveness with the material of the container body of the reagent container and does not leak. Examples of such materials include polyolefin resins and Teflon (registered trademark) materials.
- additive-free grade polyolefin-based resins and Teflon (registered trademark) -based materials are preferable from the viewpoint of reducing elution when coming into contact with the reagent to be stored.
- polyolefin-based resin polypropylene and polyethylene are preferable, and among them, from the viewpoint of maintaining the strength of the plug and the water vapor barrier property, high-density polyethylene, a mixture of polypropylene and low-density polyethylene, and the like are more preferable.
- the nozzle penetrating portion and the plug main body may have a structure that can be in close contact with each other and prevent leakage and contamination of the reagent from the container.
- the adhesion is improved by contraction of the nozzle penetrating portion.
- a compatibilizer such as a low molecular weight olefin (for example, acid-modified polyolefin resin) or a block copolymer to the material of the nozzle penetration part, the adhesion between the nozzle penetration part and the plug body can be improved. It can be improved further.
- the plug body and the nozzle penetrating portion can be manufactured by a known method such as extrusion molding, injection molding, or blow molding. In the case of injection molding, insert molding and two-color molding are suitable.
- the nozzle penetration portion may not be exposed to the environment until the nozzle is inserted. Accordingly, the reagent container is provided in a state where the plug is sealed so as to cover the nozzle penetrating portion or the plug is covered with a cover, a cap, etc., and the cover or cap is removed during use. May be.
- Nozzle The nozzle inserted into the nozzle penetration part has a material and a shape that can penetrate the nozzle penetration part having the Shore hardness described above.
- the shape of the nozzle is not particularly limited, but is preferably a hollow needle, a pointed closed needle having a horizontal hole, a needle whose tip is polished into a lancet point shape, a semi-lancet point shape, a backcut point shape, etc. It is more preferable that The shape of the nozzle tip is not particularly limited as long as it can penetrate the nozzle penetrating portion, and examples thereof include a conical shape, a triangular conical shape, and a rectangular conical shape.
- the reagent supply device of the present invention preferably includes a guide or a stopper for controlling the insertion distance of the nozzle into the nozzle penetration portion.
- the nozzle is preferably provided in the reagent supply apparatus of the present invention. Furthermore, a tube or a channel for fluidly connecting a reagent container to a later-described liquid delivery device or sample analysis unit and feeding a liquid reagent may be connected to the nozzle.
- the support member includes one or a plurality of nozzles, and each nozzle has a tip end portion facing upward, and when the reagent container is supported by the support member, the nozzle penetration portion of each reagent container is provided. It is arranged at a position where it can penetrate.
- each nozzle is simultaneously inserted into the nozzle penetration portion of each reagent container, and the nozzle insertion distance is controlled, so that the nozzle is inserted into the container. Prevent it from going too deep or falling out of the container. Furthermore, each nozzle is connected in advance with a tube connected to a liquid feeding device or a sample analysis unit. When the nozzle is inserted into a reagent container, a reagent can be supplied to the sample analysis unit.
- the reagent supply port is arranged on the lower side of the reagent container main body, the reagent inside the container main body has a nozzle installed in the nozzle penetration part of the reagent supply port. And flows out downward of the container.
- the liquid reagent flowing out from the nozzle is supplied to a sample analysis unit that analyzes the sample using the reagent. Therefore, in a preferred embodiment, the reagent container of the reagent supply apparatus of the present invention is disposed at a position higher than the sample analysis unit to which the reagent is to be supplied, and the sample analysis unit is configured using the pressure of the liquid reagent falling and flowing out. Supply reagents to.
- the reagent supply device of the present invention is used in combination with a liquid delivery device that actively transports a liquid reagent, such as a pump or a valve, and supplies the reagent to the sample analysis unit.
- the liquid delivery device may control the supply of a plurality of reagents in common, but in order to supply a plurality of reagents necessary for analysis in a timely manner, each liquid reagent supplied from the reagent supply device is independent of each other. It is desirable that a liquid feeding device is provided. Of course, it is also possible to send the reagent by the pressure of the falling and flowing out of the liquid reagent and the driving force of the liquid feeding device by combining both configurations. Therefore, in a further aspect, the reagent supply device of the present invention further includes one or more liquid delivery devices.
- Sample analyzer The reagent supply device of the present invention may be an independent device itself, but the reagent supply constituting the sample analyzer for analyzing the sample using the reagent supplied from the device It may be a unit.
- the sample analyzer includes a reagent supply unit that is the reagent supply device of the present invention, a sample analysis unit that processes or analyzes a sample using the supplied liquid reagent, and a liquid feeding device as necessary, Various components necessary for analysis such as a power source and an operation unit may be provided. Examples of the sample analyzer provided by the present invention include a liquid chromatograph, a blood sample analyzer, and a clinical laboratory instrument.
- a series of liquid reagents necessary for analysis in the apparatus is provided as a case or housing that houses one or a plurality of reagent containers each containing each reagent.
- the reagent supply unit is provided with a support member that stably supports the case or housing. When the case or housing is set in the reagent supply unit, the support member functions to cause the reagent container in the case or housing to move.
- a reagent supply port is disposed on the lower side of the reagent container.
- the reagent supply unit also includes nozzles corresponding to the nozzle penetration portions of the reagent supply ports of the reagent containers in the case or the housing, and the liquid inside the reagent container can be obtained by inserting the nozzles into the nozzle penetration portions.
- the reagent can be supplied to the outside.
- the support member and the nozzle are integrated, and when the case or housing is set in the reagent supply unit, the reagent supply port is disposed downward and the nozzle is positioned at a position corresponding to the nozzle penetration portion. Arranged, the nozzle penetrates the nozzle penetrating portion, and the liquid reagent inside the reagent container can be supplied to the outside.
- the reagent container is fluidly connected to the sample analysis unit via the nozzle and, if necessary, the liquid feeding device, and necessary reagents are supplied from the reagent supply unit to the sample analysis unit. If the fluid connection and the liquid delivery device are provided independently for each reagent, it is possible to supply the necessary amount of the necessary reagent to the sample analysis unit at the necessary timing.
- the reagent supply unit is disposed on the sample analysis unit.
- the nozzle penetrating portion of the reagent supply device of the present invention is excellent in durability, as long as the same analysis is continued, it is usually unnecessary to replace the reagent container until the reagent in the container is used up.
- the sample analyzer using the reagent supply device of the present invention can always perform a highly accurate analysis because an accurate amount of reagent is supplied even if the remaining amount of the reagent is small.
- the analyzer is a device that uses blood as a sample, separates and detects a hemoglobin component (such as hemoglobin A1c) in the blood, and measures the amount of the component, and can be used as a diabetes test device or the like.
- a hemoglobin component such as hemoglobin A1c
- the analyzer is a device that uses blood as a sample, separates and detects a hemoglobin component (such as hemoglobin A1c) in the blood, and measures the amount of the component, and can be used as a diabetes test device or the like.
- a solution for hemolysis or dilution of a blood sample a washing solution for washing the flow path of the sample, an eluent for separating a detection target component from the sample solution, etc.
- One liquid reagent is used.
- one reagent may serve a plurality of uses.
- a solution for hemolysis or dilution and a washing solution may be the same reagent.
- a plurality of different reagents may be used for one application, for example, a plurality of reagents may be used for hemolysis and sample dilution, or a plurality of reagents may be used as a washing solution or an eluent.
- a liquid reagent is a solution for hemolysis or dilution of a blood sample and a reagent (hemolysis / dilution / washing liquid) that is a washing liquid for washing the flow path of the sample, and one or more kinds thereof. Eluents are used.
- the at least one liquid reagent is filled in a separate reagent container.
- the reagent containers filled with the liquid reagent may all have the same shape or volume, but may have different shapes or volumes.
- each reagent container needs only to be large enough to be filled with about 100 mL of liquid reagent, but when a large amount of reagent is required for analysis, such as when using a large amount of washing solution, dilution solution, or eluent, A larger capacity of about 500 mL can also be configured.
- the at least one liquid reagent can be provided as a reagent kit housed in one housing.
- the reagent kit is set on a support member provided on the upper part of the blood sample analyzer main body so that the reagent supply port of each of the plurality of reagent containers accommodated faces downward.
- the support member is a recess that supports the reagent container together with the reagent kit, and includes a nozzle at a position corresponding to the nozzle penetration portion of the plurality of reagent supply ports.
- the device can be supplied.
- This blood sample analyzer is provided with a liquid delivery device, such as a pump and / or a valve, for controlling the liquid delivery in each liquid reagent channel.
- a liquid delivery device such as a pump and / or a valve, for controlling the liquid delivery in each liquid reagent channel.
- Example 1 Reagent supply device (production of laminate)
- Additive-grade low-density polyethylene film (thickness 130 ⁇ m), polyethylene terephthalate film (thickness 12 ⁇ m), nylon film (thickness 25 ⁇ m), aluminum foil (thickness 15 ⁇ m), and polyethylene terephthalate film (thickness 12 ⁇ m) Are laminated in this order to obtain a laminate.
- the low density polyethylene film and the polyethylene terephthalate film were bonded while extrusion lamination of the low density polyethylene.
- the polyethylene terephthalate film, the nylon film, the aluminum foil, and the polyethylene terephthalate film were bonded by a dry laminating method using a linear polymer polyester as an adhesive.
- a flexible bag was prepared so that the above-mentioned additive-free grade low-density polyethylene film was the innermost layer.
- a mouth part (diameter 1 cm) was produced by injection-molding additive-free grade polyethylene, and heat-sealed to the bag to produce a reagent container main body.
- the plug body was manufactured using a mixed material of polypropylene and low density polyethylene. According to JIS G 0202, the Rockwell hardness of the plug body was measured using a Rockwell hardness tester. The nozzle penetration part was manufactured using the olefin elastomer. According to JIS Z 2246, the Shore hardness of this nozzle penetration part was measured using the Shore hardness tester. The Rockwell hardness of the plug body was R80 to 90, and the Shore hardness of the nozzle penetrating portion was in the range of A40 ° to A50 °. A plug having a nozzle penetrating portion was manufactured by two-color molding using a two-color molding apparatus.
- FIG. 1 shows a schematic diagram of one embodiment of a reagent container used in the present invention manufactured by the above procedure.
- the reagent container 1 includes a container body 2 in which a mouth portion 4 is attached to a flexible bag 3 and a reagent supply port 5.
- FIG. 2 is a schematic diagram of a cross section of the reagent supply port 5 shown in FIG.
- the reagent supply port 5 is a plug having a plug body 5a and a nozzle penetrating portion 5b, and is closed until the reagent is used.
- the nozzle penetrating portion 5b is disposed below the reagent supply port 5 as shown in FIG.
- the plug body 5a and the nozzle penetrating part 5b are in close contact with each other by flange bonding, and the mouth part 4 and the reagent supply port 5 of the container body have threads and are screwed together. Thereby, even when the reagent supply port 5 is directed downward, leakage of the liquid reagent from the reagent container 1 is prevented.
- FIG. 3 is a schematic view showing a cross section of an embodiment of a housing that houses the reagent container 1 shown in FIG.
- the housing 6 shown in FIG. 3 stores a plurality of reagent containers 1 having different capacities, and the plurality of reagent containers 1 are stored in the housing 6 in an unequal arrangement.
- the reagent container 1 accommodated in the housing 6 has the container main body part inside, but the reagent supply port 5 is exposed to the outside, and the nozzle can be inserted.
- FIG. 4 is a schematic view showing an embodiment of a support member for supporting a reagent container in the reagent supply apparatus of the present invention.
- the support member 7 is formed on the analyzer main body 10 for performing analysis using the reagent supplied from the reagent supply device of the present invention, and the reagent container 1 together with the housing 6 shown in FIG. It is comprised so that it may support.
- the support member 7 also has holes 8 for fitting the reagent supply ports 5 of the reagent container 1, and nozzles 9 are arranged in the respective holes 8 with the pointed ends facing upward.
- the reagent supply port 5 exposed from the housing 6 fits into each hole 8, whereby the nozzle 9 is inserted into the nozzle penetration portion 5 b.
- the hole 8 also has a function as a guide for controlling the introduction distance of the nozzle 9 with respect to the reagent container 1 to prevent the nozzle 9 from entering the reagent container 1 too far or dropping out of the reagent container 1.
- FIG. 5 is a schematic diagram showing an embodiment of the reagent supply apparatus 11 of the present invention connected to a sample analysis unit.
- the reagent container 1 is supported by a support member 7 that is a hole having a nozzle 9, and is arranged with the reagent supply port 5 facing downward.
- the reagent in the reagent container is supplied to the sample analysis unit 13 from the nozzle 9 inserted into the nozzle penetration part 5 b of the reagent supply port 5 via the liquid feeding device 12.
- FIG. 6 shows a schematic diagram of a blood sample analyzer 100 by liquid chromatography incorporating the reagent supply device 110 of the present invention.
- the support member 113 of the reagent supply device 110 is formed on the main body of the analysis device 100 that performs analysis using the reagent supplied from the reagent supply device 110, as in FIG. 4.
- a housing 120 containing a container is set. The housing 120 accommodates three reagent containers 111 each having a flexible container body.
- Each reagent container 111 includes a reagent for hemolysis or dilution of a blood sample and a reagent (haemolysis / dilution / washing liquid: 111a) that is a washing liquid for washing the flow path of the sample, and two types of eluents (111b). And 111c) are filled respectively.
- a reagent container 111 accommodated in the housing 120 is arranged with the reagent supply port 112 facing downward.
- the support member 113 has holes 114, and each hole 114 has a nozzle 115 having a pointed upward.
- each reagent container 111 When the housing 120 is set on the support member 113, the reagent supply port 112 of each reagent container is fitted into each hole 114, and the nozzle 115 is inserted into the container 111 from the nozzle penetration portion of the reagent supply port 112. The internal reagent can be taken out of the container through the nozzle 115.
- the reagent contained in each reagent container 111 is supplied to the sample injection part B or the chromatography column C of the sample analyzer.
- the supply speed and amount of each reagent are controlled by pumps 130a to 130c installed for each reagent.
- the reagent used for the analysis is recovered as a waste liquid via the drainage flow path 140.
- the blood sample is set in the sample injection part B.
- the hemolyzing / diluting / washing liquid 111a is supplied to the sample injection part B, and hemolysis and dilution of the hemolyzed sample are performed.
- a predetermined amount of the diluted sample is injected into the flow of the eluents 111b and 111c from the sample injection unit. Thereafter, when the diluted sample is sent to the column C by the flow of the eluents 111b and 111c, the detection target component is separated by the column and detected by the detection unit D.
- Reagent Container 2 Container Body 3 Bag 4 Mouth 5 Reagent Supply Port (Plug) 5a Plug body 5b Nozzle penetrating portion 6 Housing 7 Support member 8 Hole 9 Nozzle 10 Analytical device body 11 Reagent supply device 12 Liquid feeding device 13 Sample analysis unit 100 Blood sample analysis device 110 Reagent supply device 111 Reagent container 111a Hemolysis / dilution / washing solution 111b Eluent b 111c Eluent c DESCRIPTION OF SYMBOLS 112 Reagent supply port 113 Support member 114 Hole 115 Nozzle 120 Housing 130a-c Pump 140 Drain flow path 150 Drain valve 160 Three-way valve A Deaeration unit B Sample injection part C Chromatography column D Detection part
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Abstract
Description
液体試薬を収納する容器本体と、該容器本体に設けられた試薬供給口とを有する試薬容器を少なくとも1つ備え、かつ
該少なくとも1つの試薬容器の各々を、該試薬供給口が該容器本体の下側に位置するように支持する支持部材を備え、
該試薬供給口は、少なくとも1つのノズル貫通部を有し、
該少なくとも1つのノズル貫通部の各々は、ショア硬さA5°~A90°を有する材料から構成されている、
試薬供給装置を提供する。
少なくとも1つの試薬容器を設置すること、
ここで該少なくとも1つの試薬容器の各々は、液体試薬を収納する容器本体と、該容器本体下側に設けられた試薬供給口とを有し、該試薬供給口は、少なくとも1つのノズル貫通部を有し、かつ該少なくとも1つのノズル貫通部の各々は、ショア硬さA5°~A90°を有する材料から構成されており、
および、
該ノズル貫通部より該試薬容器内部へ挿入したノズルから、該液体試薬を下方向へ流出させ、次いで試料分析ユニットへと送液すること
を含む方法を提供する。
少なくとも1つの試薬容器を設置すること、
ここで該少なくとも1つの試薬容器の各々は、液体試薬を収納する容器本体と、該容器本体下側に設けられた試薬供給口とを有し、該試薬供給口は、少なくとも1つのノズル貫通部を有し、かつ該少なくとも1つのノズル貫通部の各々は、ショア硬さA5°~A90°を有する材料から構成されており、
該ノズル貫通部より該試薬容器内部へ挿入したノズルから、該液体試薬を下方向へ流出させ、次いで試料分析ユニットへと送液すること、および
該送液された液体試薬により試料を処理または分析すること、
を含む方法を提供する。
本発明の試薬供給装置は、1つまたは複数の試薬容器を備える。該試薬容器の各々は、同じかまたは異なる種類の液体試薬を収納しており、該試薬容器の数や収納される試薬の種類は、当該試薬が使用される分析方法に応じて適宜選択することができる。該試薬容器の各々は、液体試薬を収納する容器本体と、該容器本体の下側に設けられた試薬供給口とを有する。
本発明の試薬供給装置はまた、上記試薬容器を支える支持部材を備える。該支持部材は、該1つもしくは複数の試薬容器をそれぞれ別個に支持する1つもしくは複数の部材を構成してもよく、または該1つもしくは複数の試薬容器をまとめて支持する1つの部材であってもよい。あるいは、該支持部材は、上述した試薬容器を収納するハウジングを支持することによって、試薬容器を支持するものであってもよい。いずれの場合も、各試薬容器は、該支持部材により、その試薬供給口が該試薬容器本体の下側に位置するように配置される。したがって、本発明の試薬供給装置においては、試薬容器内部の液体試薬は、該容器の下側から下方に流出することになる。この構成をとることにより、従来のような液体試薬の吸引は不要となるため、吸引による液体試薬への気泡の混入が防止される。したがって、本発明の試薬供給装置では、試薬容器として可撓性容器を用いた場合に、従来の試薬容器設置手順では必要であった使用前に試薬容器から空気を抜く操作が不要であり、しかも容器内の試薬を最後まで使い切ることが可能になる。
上記試薬容器の試薬供給口は、未使用状態においては開口しておらず、ノズルの貫通を可能にする1つまたは複数のノズル貫通部を有している。例えば、試薬供給口は、試薬容器に取り付けられたプラグであり、該プラグは、1つまたは複数のノズル貫通部を有する。該ノズル貫通部の一端は容器外部に向いた外向面であり、それと対向する端は容器内部に向いた内向面である。該外向面に挿入されたノズルは、該内向面へと貫通することができる。斯くして、ノズルは試薬供給口を封鎖するプラグを貫通し、該ノズルを介して容器内の液体試薬が外部に供給される。したがって、本発明の試薬供給装置においては、試薬容器を開封することなく、密閉された試薬容器内部にノズルを貫通させて試薬を供給するので、容器の開封に伴う試薬のコンタミネーションや、使用者と試薬との接触を防止することができる。
該ノズル貫通部に挿入されるノズルは、上述したショア硬さを有するノズル貫通部を貫通することができる材料および形状を有する。ノズルの形状は特に制限されないが、中空針であることが好ましく、横穴を有する尖端密閉型の針、針の先端がランセットポイント形状、セミランセットポイント形状、バックカットポイント形状等に研磨された針等であることがより好ましい。該ノズル尖端の形状は、該ノズル貫通部を貫通できれば特に制限されず、円すい型、三角すい型、四角すい型などが例示できる。該ノズルの材料としては、ノズル貫通部の貫通性および試薬への溶出性などの観点から、金属、樹脂、セラミック等が好ましい。試薬の使い残りを防止する観点からは、該ノズルは、該ノズル貫通部を貫通して容器本体内部に挿入した後は、できるだけ容器本体の底部に近い部分にその上端が留まっていることが望ましい。一方で、該ノズルを挿入する距離が短いとノズル脱落の恐れがある。したがって、本発明の試薬供給装置は、該ノズル貫通部への該ノズルの挿入距離を制御するガイドまたはストッパーを備えていることが好ましい。
本発明の試薬供給装置においては、試薬容器本体の下側に試薬供給口が配置されているので、容器本体内部の試薬は、該試薬供給口のノズル貫通部に設置されたノズルを介して、容器下方へと流出する。当該ノズルから流出した液体試薬は、当該試薬を使用して試料の分析を行う試料分析ユニットへと供給される。したがって、好ましい一態様において、本発明の試薬供給装置の試薬容器は、試薬を供給すべき試料分析ユニットよりも高い位置に配置され、液体試薬の落下流出の圧力を利用して、該試料分析ユニットに試薬を供給する。別の好ましい一態様においては、本発明の試薬供給装置は、液体試薬を能動的に輸送する送液装置、例えばポンプ、弁などと組み合わせて使用されて、該試料分析ユニットに試薬を供給する。送液装置は複数の試薬の供給を共通に制御してもよいが、分析に必要な複数の試薬を適時供給するためには、試薬供給装置から供給される各液体試薬に対して各々独立の送液装置が設けられていることが望ましい。両構成を組み合わせて、液体試薬の落下流出の圧力と送液装置の駆動力とによって試薬を送液することも当然可能である。したがって、さらなる態様において、本発明の試薬供給装置は、1つまたは複数の送液装置をさらに備えている。
本発明の試薬供給装置は、それ自体で独立した装置であってもよいが、当該装置から供給される試薬を使用して試料の分析を行うための試料分析装置を構成する試薬供給ユニットであってもよい。該試料分析装置は、本発明の試薬供給装置である試薬供給ユニット、供給される液体試薬を使用して試料の処理または分析を行う試料分析ユニット、および必要に応じて送液装置を備え、さらに電源、操作部などの分析に必要な各種構成要素を備えていてもよい。本発明により提供される試料分析装置としては、液体クロマトグラフ、血液試料分析装置、臨床検査機器などが挙げられる。
本発明の試薬供給装置を組み込んだ試料分析装置の具体例として、液体クロマトグラフィーを利用した血液試料分析装置について説明する。当該分析装置は、血液を試料とし、当該血液中のヘモグロビン成分(ヘモグロビンA1cなど)を分離および検出してその成分量を計測する装置であり、糖尿病検査機器などとして利用することができる。本分析には、血液試料の溶血または希釈のための溶液、試料の流路の洗浄を行うための洗浄液、試料溶液から検出対象成分を分離するための溶離液などからなる群より選択される少なくとも1種の液体試薬が使用される。これらの試薬は、1つの試薬が複数の用途を兼ねていてもよく、例えば、溶血または希釈のための溶液と洗浄液とが同じ試薬であってもよい。あるいは、1つの用途に複数の異なる試薬を使用してもよく、例えば、溶血や試料の希釈に複数の試薬を使用したり、または洗浄液もしくは溶離液として複数の試薬を使用したりしてもよい。一実施形態においては、液体試薬として、血液試料の溶血または希釈のための溶液および試料の流路の洗浄を行うための洗浄液である試薬(溶血・希釈・洗浄液)と、1種または2種以上の溶離液とが使用される。
(積層体の作製)
無添加グレードの低密度ポリエチレンフィルム(厚さ130μm)と、ポリエチレンテレフタレートフィルム(厚さ12μm)と、ナイロンフィルム(厚さ25μm)と、アルミニウム箔(厚さ15μm)と、ポリエチレンテレフタレートフィルム(厚さ12μm)とをこの順番に積層することによって、積層体を得た。なお、低密度ポリエチレンフィルムと、ポリエチレンテレフタレートフィルムとは、低密度ポリエチレンを押出ラミネートしながら接着した。また、ポリエチレンテレフタレートフィルムと、ナイロンフィルムと、アルミニウム箔と、ポリエチレンテレフタレートフィルムとは、接着剤として、直鎖状高分子ポリエステルを用いて、ドライラミネート法により接着した。
得られた積層体を用いて、上述の無添加グレードの低密度ポリエチレンフィルムが最内層となるように、可撓性のバッグを作製した。次いで、無添加グレードのポリエチレンを射出成型することにより口部(直径1cm)を作製し、上記バッグに熱融着して、試薬容器本体を作製した。
プラグ本体は、ポリプロピレンと低密度ポリエチレンの混合材料を用いて製造した。JIS G 0202に従って、ロックウェル硬さ試験機を用いてこのプラグ本体のロックウェル硬さを測定した。ノズル貫通部は、オレフィン系エラストマーを用いて製造した。JIS Z 2246に従って、ショア硬さ試験機を用いてこのノズル貫通部のショア硬さを測定した。プラグ本体のロックウェル硬さはR80~90、ノズル貫通部のショア硬さはA40°~A50°の範囲内であった。二色成形装置を用いた二色成形により、ノズル貫通部を有するプラグを製造した。
本発明の別の実施形態として、図6に、本発明の試薬供給装置110を組み込んだ、液体クロマトグラフィーによる血液試料分析装置100の模式図を示す。図6において、試薬供給装置110の支持部材113は、図4と同様、試薬供給装置110から供給される試薬を用いて分析を行う分析装置100の本体上に形成されており、ここに、試薬容器を収納したハウジング120がセットされる。ハウジング120には、可撓性容器本体を有する試薬容器111が3つ収納されている。各試薬容器111には、血液試料の溶血または希釈のための溶液および試料の流路の洗浄を行うための洗浄液である試薬(溶血・希釈・洗浄液:111a)、および2種類の溶離液(111bおよび111c)がそれぞれ充填されている。ハウジング120に収納された各試薬容器111は、試薬供給口112を下側に向けた状態で配置される。
支持部材113は穴114を有しており、各穴114は尖端が上方に向いたノズル115を有する。ハウジング120が支持部材113にセットされると、各試薬容器の試薬供給口112が各穴114に嵌り、ノズル115が試薬供給口112のノズル貫通部から容器111内部に挿入されるので、容器111内部の試薬をノズル115を介して容器外部に取り出すことが可能になる。
血液試料分析装置100を作動させると、各試薬容器111に収容されている試薬が試料分析装置の試料注入部BまたはクロマトグラフィーカラムCに供給される。各試薬の供給速度や量は、各試薬ごとに設置されたポンプ130a~cにより制御される。分析に用いられた試薬は、排液流路140を介して、廃液として回収される。
2 容器本体
3 バッグ
4 口部
5 試薬供給口(プラグ)
5a プラグ本体
5b ノズル貫通部
6 ハウジング
7 支持部材
8 穴
9 ノズル
10 分析装置本体
11 試薬供給装置
12 送液装置
13 試料分析ユニット
100 血液試料分析装置
110 試薬供給装置
111 試薬容器
111a 溶血・希釈・洗浄液
111b 溶離液b
111c 溶離液c
112 試薬供給口
113 支持部材
114 穴
115 ノズル
120 ハウジング
130a~c ポンプ
140 排液流路
150 ドレインバルブ
160 三方バルブ
A 脱気ユニット
B 試料注入部
C クロマトグラフィーカラム
D 検出部
Claims (18)
- 試薬供給装置であって、該装置は、
液体試薬を収納する容器本体と、該容器本体に設けられた試薬供給口とを有する試薬容器を少なくとも1つ備え、かつ
該少なくとも1つの試薬容器の各々を、該試薬供給口が該容器本体の下側に位置するように支持する支持部材を備え、
該試薬供給口は、少なくとも1つのノズル貫通部を有し、
該少なくとも1つのノズル貫通部の各々は、ショア硬さA5°~A90°を有する材料から構成されている、
試薬供給装置。 - 前記液体試薬を試料分析ユニットへ送液するための少なくとも1つの送液装置をさらに備える、請求項1記載の試薬供給装置。
- 前記試薬容器が試料分析ユニットよりも高い位置に配置される、請求項1又は2記載の試薬供給装置。
- 前記支持部材がノズルを備え、該ノズルは前記試薬容器のノズル貫通部に対応する位置に配置されている、請求項1~3のいずれか1項記載の試薬供給装置。
- 前記試薬容器を前記支持部材で支持したときに、前記試薬容器のノズル貫通部にノズルが挿入される、請求項1~4のいずれか1項記載の試薬供給装置。
- 前記ショア硬さA5°~A90°を有する材料がオレフィン系エラストマーまたはスチレン系エラストマーである、請求項1~5のいずれか1項記載の試薬供給装置。
- プラグ本体と前記少なくとも1つのノズル貫通部とにより構成される試薬供給口において、該プラグ本体と該ノズル貫通部とがフランジ接合されている、請求項1~6のいずれか1項記載の試薬供給装置。
- 前記少なくとも1つの試薬容器を均等な配置で収納するハウジングをさらに有する、請求項1~7のいずれか1項記載の試薬供給装置。
- 前記少なくとも1つの試薬容器を不均等な配置で収納するハウジングをさらに有する、請求項1~7のいずれか1項記載の試薬供給装置。
- 前記支持部材が、前記ハウジングを支持することによって前記試薬容器を支持する、請求項8又は9記載の試薬供給装置。
- 前記液体試薬が、液体クロマトグラフィー用の試薬である、請求項1~10のいずれか1項記載の試薬供給装置。
- 請求項1~11のいずれか1項記載の試薬供給装置と、試料分析ユニットとを備える試料分析装置。
- 試料分析ユニットへの試薬供給方法であって、
少なくとも1つの試薬容器を設置すること、
ここで該少なくとも1つの試薬容器の各々は、液体試薬を収納する容器本体と、該容器本体下側に設けられた試薬供給口とを有し、該試薬供給口は、少なくとも1つのノズル貫通部を有し、かつ該少なくとも1つのノズル貫通部の各々は、ショア硬さA5°~A90°を有する材料から構成されており、
および、
該ノズル貫通部より該試薬容器内部へ挿入したノズルから、該液体試薬を下方向へ流出させ、次いで試料分析ユニットへと送液すること
を含む方法。 - 試料分析方法であって、
少なくとも1つの試薬容器を設置すること、
ここで該少なくとも1つの試薬容器の各々は、液体試薬を収納する容器本体と、該容器本体下側に設けられた試薬供給口とを有し、該試薬供給口は、少なくとも1つのノズル貫通部を有し、かつ該少なくとも1つのノズル貫通部の各々は、ショア硬さA5°~A90°を有する材料から構成されており、
該ノズル貫通部より該試薬容器内部へ挿入したノズルから、該液体試薬を下方向へ流出させ、次いで試料分析ユニットへと送液すること、および
該送液された液体試薬により試料を処理または分析すること、
を含む方法。 - 前記液体試薬の試料分析ユニットへの送液が、少なくとも1つの送液装置により制御される、請求項13又は14記載の方法。
- 前記少なくとも1つの試薬容器が、試料分析ユニットよりも高い位置に配置される、請求項13~15のいずれか1項記載の方法。
- 前記液体試薬が、液体クロマトグラフィー用の試薬である、請求項13~16のいずれか1項記載の方法。
- 前記試料が血液であり、前記分析が液体クロマトグラフィーであり、前記液体試薬が、血液試料の溶血または希釈のための溶液、洗浄液、および溶離液からなる群より選択される少なくとも1種である、請求項14~16のいずれか1項記載の方法。
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EP14830223.5A EP3026437B1 (en) | 2013-07-26 | 2014-07-25 | Sample analysis device |
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CN201480042007.8A CN105474019A (zh) | 2013-07-26 | 2014-07-25 | 试剂供给装置 |
US14/908,073 US9889447B2 (en) | 2013-07-26 | 2014-07-25 | Reagent supplying device |
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CN (2) | CN105474019A (ja) |
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EP3026437A4 (en) | 2017-03-01 |
CN111474375A (zh) | 2020-07-31 |
CN105474019A (zh) | 2016-04-06 |
US9889447B2 (en) | 2018-02-13 |
EP3026437A1 (en) | 2016-06-01 |
US20160184826A1 (en) | 2016-06-30 |
PT3026437T (pt) | 2020-05-22 |
JP6504566B2 (ja) | 2019-04-24 |
EP3026437B1 (en) | 2020-04-29 |
JPWO2015012391A1 (ja) | 2017-03-02 |
ES2791366T3 (es) | 2020-11-04 |
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