WO2015004403A3 - Procede de culture cellulaire decouple - Google Patents

Procede de culture cellulaire decouple Download PDF

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Publication number
WO2015004403A3
WO2015004403A3 PCT/FR2014/051809 FR2014051809W WO2015004403A3 WO 2015004403 A3 WO2015004403 A3 WO 2015004403A3 FR 2014051809 W FR2014051809 W FR 2014051809W WO 2015004403 A3 WO2015004403 A3 WO 2015004403A3
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WO
WIPO (PCT)
Prior art keywords
cell culture
culture method
conditions
uncoupled
uncoupled cell
Prior art date
Application number
PCT/FR2014/051809
Other languages
English (en)
Other versions
WO2015004403A2 (fr
Inventor
Patrice Garnier
Julien PAGLIARDINI
Pierre Calleja
Original Assignee
Fermentalg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fermentalg filed Critical Fermentalg
Priority to ES14747100T priority Critical patent/ES2704903T3/es
Priority to EP14747100.7A priority patent/EP3019593B1/fr
Priority to JP2016524883A priority patent/JP2016523554A/ja
Priority to US14/904,381 priority patent/US20160145668A1/en
Publication of WO2015004403A2 publication Critical patent/WO2015004403A2/fr
Publication of WO2015004403A3 publication Critical patent/WO2015004403A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G33/00—Cultivation of seaweed or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de production de biomasse par culture cellulaire en conditions de mixotrophie, notamment en présence d'un éclairement discontinu et/ou variable de lumière, et/ou en conditions d'hétérotrophie permettant d'obtenir à la fois une augmentation de la concentration cellulaire et de la production de molécules d'intérêt.
PCT/FR2014/051809 2013-07-12 2014-07-15 Procede de culture cellulaire decouple WO2015004403A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES14747100T ES2704903T3 (es) 2013-07-12 2014-07-15 Procedimiento de cultivo celular desacoplado
EP14747100.7A EP3019593B1 (fr) 2013-07-12 2014-07-15 Procede de culture cellulaire decouple
JP2016524883A JP2016523554A (ja) 2013-07-12 2014-07-15 不連結細胞培養方法
US14/904,381 US20160145668A1 (en) 2013-07-12 2014-07-15 Uncoupled cell culture method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1356918 2013-07-12
FR1356918A FR3008422B1 (fr) 2013-07-12 2013-07-12 Procede de culture cellulaire decouple

Publications (2)

Publication Number Publication Date
WO2015004403A2 WO2015004403A2 (fr) 2015-01-15
WO2015004403A3 true WO2015004403A3 (fr) 2015-04-02

Family

ID=49667306

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2014/051809 WO2015004403A2 (fr) 2013-07-12 2014-07-15 Procede de culture cellulaire decouple

Country Status (6)

Country Link
US (1) US20160145668A1 (fr)
EP (1) EP3019593B1 (fr)
JP (1) JP2016523554A (fr)
ES (1) ES2704903T3 (fr)
FR (1) FR3008422B1 (fr)
WO (1) WO2015004403A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3025214A1 (fr) * 2014-08-26 2016-03-04 Fermentalg Nouveau procede de culture d'algues, particulierement de microalgues
FR3025215A1 (fr) * 2014-08-27 2016-03-04 Fermentalg Nouveau procede de culture de microalgues
IL293920A (en) * 2015-04-13 2022-08-01 Algatechnologies Ltd Mixtures containing carotenoids and their uses
FR3038914B1 (fr) * 2015-07-17 2020-03-13 Fermentalg Biomasse de thraustochytrides, procede de culture et utilisations
FR3053052B1 (fr) 2016-06-28 2021-02-12 Fermentalg Microalgue modifiee pour une production enrichie en tag
JP6722209B2 (ja) * 2017-01-24 2020-07-15 長瀬産業株式会社 カロテノイド組成物、カロテノイド組成物の製造方法、カロテノイド組成物を生産する微生物
DE102017218001B4 (de) 2017-10-10 2022-06-02 GICON GROßMANN INGENIEUR CONSULT GMBH Verfahren und System zur heterotrophen und mixotrophen Kultivierung von Mikroalgen
FR3085825B1 (fr) 2018-09-14 2021-07-16 Fermentalg Huile de microorganismes riche en acide docosahexaenoique
FR3085962B1 (fr) 2018-09-14 2021-06-18 Fermentalg Procede d'extracton d'une huile riche en pufa
CN112063602B (zh) * 2020-09-03 2022-10-21 宁夏中微泰克生物技术有限责任公司 一种亚洲小车蝗小G蛋白质Ras及其编码基因与应用
FR3120308B1 (fr) 2021-03-02 2024-03-01 Univ Nantes Utilisation d’exopolysaccharides de micro-algues a titre d’agents texturants
CN114134050B (zh) * 2021-12-01 2023-09-15 清远一生自然生物研究院有限公司 一株积累DHA和β-胡萝卜素的裂殖壶菌FR-908及其制备方法与应用
CN114134049B (zh) * 2021-12-01 2023-08-25 清远一生自然生物研究院有限公司 一株联产DHA和β-胡萝卜素的裂殖壶菌SL-916及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090209014A1 (en) * 2008-02-20 2009-08-20 Zhanyou Chi Heterotrophic algal high cell density production method and system
EP2559342A1 (fr) * 2011-08-16 2013-02-20 Fermentalg Procédé d'amélioration de la valeur nutritive de mollusques filtreurs, en particulier des huîtres, par stabulation en présence de microalgues

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7905930B2 (en) * 2006-12-29 2011-03-15 Genifuel Corporation Two-stage process for producing oil from microalgae
US8765460B2 (en) * 2009-12-14 2014-07-01 Atle B. Nordvik Photobioreactor system for mass production of microorganisms
JP2012000026A (ja) * 2010-06-15 2012-01-05 Fujifilm Corp 光合成微生物の付着培養方法
FR2964667B1 (fr) * 2010-09-15 2014-08-22 Fermentalg Procede de culture d'algues unicellulaires mixotrophes en presence d'un apport lumineux discontinu sous forme de flashs
JP2012157274A (ja) * 2011-01-31 2012-08-23 Fujifilm Corp 光合成微生物の付着培養方法
FR2976292B1 (fr) * 2011-06-08 2015-01-02 Fermentalg Nouvelle souche de microalgue du genre odontella pour la production d'epa et de dha en mode de culture mixotrophe
TWI551216B (zh) * 2011-08-05 2016-10-01 Showa Denko Kk Algae culture method and algae culture device
JP5810831B2 (ja) * 2011-10-26 2015-11-11 株式会社デンソー 藻類の培養方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090209014A1 (en) * 2008-02-20 2009-08-20 Zhanyou Chi Heterotrophic algal high cell density production method and system
EP2559342A1 (fr) * 2011-08-16 2013-02-20 Fermentalg Procédé d'amélioration de la valeur nutritive de mollusques filtreurs, en particulier des huîtres, par stabulation en présence de microalgues

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MPHO SETLHAKU ET AL: "Improvement in the bioreactor specific productivity by coupling continuous reactor with repeated fed-batch reactor for acetone-butanol-ethanol production", JOURNAL OF BIOTECHNOLOGY, vol. 161, no. 2, 1 October 2012 (2012-10-01), pages 147 - 152, XP055110168, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2012.04.004 *

Also Published As

Publication number Publication date
EP3019593B1 (fr) 2018-10-17
JP2016523554A (ja) 2016-08-12
EP3019593A2 (fr) 2016-05-18
ES2704903T3 (es) 2019-03-20
FR3008422B1 (fr) 2017-11-17
FR3008422A1 (fr) 2015-01-16
WO2015004403A2 (fr) 2015-01-15
US20160145668A1 (en) 2016-05-26

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