Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C9/00—Milk preparations; Milk powder or milk powder preparations
  • A23C9/20—Dietetic milk products not covered by groups A23C9/12 - A23C9/18
  • A23C9/206—Colostrum; Human milk
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C13/00—Cream; Cream preparations; Making thereof
  • A23C13/12—Cream preparations
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
  • A23C9/00—Milk preparations; Milk powder or milk powder preparations
  • A23C9/20—Dietetic milk products not covered by groups A23C9/12 - A23C9/18

Definitions

• the present disclosure relates generally to high fat human milk products, such as standardized human cream compositions, methods of producing the compositions, and methods of using the compositions.
• Human breast milk is considered the nutrition of choice for infants, including those in the neonatal intensive care unit (NICU) ( American Academy of Pediatrics, Section on Breastfeeding. Breastfeeding and the Use of Human Milk. Pediatrics, 1 15:496-506, 2005).
• NICU neonatal intensive care unit
• the human milk used in the NICU is from the baby's own mother, but it may also come from a donor.
• VLBW very-low birth weight
• the milk is often supplemented with a human milk fortifier in order to provide the extra nutrition that these babies need.
• TPN is effective at delivering the required level of nutrition to VLBW infants, it is also associated with many negative side effects including, for example, liver damage.
• the current invention solves the problem by providing a pasteurized, high fat human milk product that can be administered eiiterally and increase the caloric content of human milk while not substantially increasing the overall volume fed to the infant.
• This disclosure features high fat human milk products, e.g., human cream compositions, as well as methods of making and methods of using the high fat human milk products.
• the disclosure features a human milk cream composition
• a human milk cream composition comprising pasteurized cream derived from human mil k, wherein the composition comprises about 2,5 kcal/ml. In one embodiment, the composition comprises about 25% fat. In another embodiment, the human cream composition further comprises human skim milk permeate. In yet another embodiment, the human cream composition further comprises deionized water.
• the composition is used for enteral nutrition.
• the enteral nutrition is for a low birth weight infant.
• the disclosure features a method of making a human milk cream composition
• a method of making a human milk cream composition comprising the steps of: obtaining a pool of human milk, separating the pool of human milk into a cream portion and a skim milk portion, formulating the cream portion to obtain a cream composition comprising about 2.5 kcal/ml, and pasteurizing the cream composition.
• the separating step is via ultracentrifugation.
• the method of making a human milk cream composition further comprises ultra filtering water from the skim milk portion, thereby obtaining a human skim milk permeate.
• the formulating step comprises adding a volume of the human skim milk permeate to the cream portion.
• the formulating step comprises adding a volume of deionized water to the cream portion.
• the method of making a human milk cream composition further comprises a step of testing the pool of human milk for adulterants, contaminants, drugs and/or pathogens.
• the testing step comprises testing using a microorganism panel.
• the testing step comprises PCR analysis for HIV, HBV and HCV.
• the testing step detects bovine protein.
• the testing step comprises testing for one or more drugs.
• the one or more drugs are selected from amphetamines, benzodiazepine, cocaine, methamphetamines, opiates, synthetic opioids (e.g. oxycodone/oxymorphone), THC, and principle metabolites thereof.
• the pool of human milk is from one or more donors.
• the disclosure features a method of increasing the caloric content of human milk to a desired caloric content level, comprising the steps of: obtaining a sample of human milk, measuring the caloric content of the human milk, determining a volume of a human milk cream composition needed to raise the caloric content of the human milk to the desired caloric content level, and adding the volume of the human milk cream composition to the container of human milk.
• the desired caloric content is 20 kcal/oz.
• the human milk cream composition comprises about 2.5 kcal/ml. In a related embodiment, the human milk cream composition comprises about 25% fat.
• the human milk is for enteral nutrition.
• the enteral nutrition is for a low birth weight infant.
• compositions and methods featured herein relate to human milk cream products.
• human milk e.g., mother's or donor
• the rationale behind supplementing human milk stems from the finding that milk from mothers who deliver significantly prematurely does not have adequate nutritional content to completely meet the increased metabolic and growth needs of their infants relative to a full-term infant (Hawthorne et a!., Minerva Pediatr, 56:359-372, 2004; Lawrence and Lawrence, Breastfeeding: A Guide for the Medical Profession, 6 th edition. Philadelphia: Elsevier Mosby, 2005; and Ziegler, Human Milk for the Preterm Infant, International Congress of the Human Milk Banking Association of North America.
• pre-term milk may contain higher levels of protein than milk from a mother who has delivered at term (Hawthorne et al., Minerva Pediatr, 56:359-372, 2004; Lawrence and Lawrence, Breastfeeding: A Guide for the Medical
• the caloric content of the human milk supplied to infants is very rarely measured. As demonstrated by the study performed by Wocjik et al. (J Am Diet Assoc, 109: 137-140, 2009), it is likely that the human milk being supplied to LBW and VLBW infants is often not providing a sufficient amount of calories to meet the nutritional needs of a pre-term infant.
• the high fat human milk compositions described herein provide a solution to this problem and may be used, e.g., to supplement human milk in order to increase the caloric content to the desired level without increasing the volume to be fed to the infant, e.g., a LBW infant. This is particularly useful when ail that is needed is increased caloric intake and not increased protein content.
• the compositions of the current in vention solve this problem by increasing calories without increasing protein and therefore provide a more cost effective solution to the problem.
• TPN Total parenteral nutrition
• TPN intravenously and bypassing the gastrointestinal tract
• the high fat human milk compositions described herein can be used to increase the caloric content and fat content of human milk, thereby providing means for enteral delivery of human milk fat. Maintaining a fully human milk based diet reduces the incidence of complications such as necrotizing enterocolitis, and therefore, it is contemplated that enteral feeds of human milk supplemented with high fat human milk products may be used in place of TPN.
• premature infants born less than 37 weeks gestational age and/or with birth weights less than 2500 g.
• LLBW 7 very-low-birth-weight infant refers to an infant with a birth weight of 1250 g or less.
• LBW infants includes VLBW infants.
• whole milk refers to human milk from which no fat has been removed.
• bioburden microbiological contaminants and pathogens
• milk (generally living) that can be present in milk, e.g., viruses, bacteria, mold, fungus and the like.
• the high fat human milk compositions, or human cream compositions, described herein are produced from whole human milk.
• the human cream composition comprises about 2.0 kcal to about 3.0 kcal or more per ml.
• the human cream composition comprises about 2.5 kcal/ml. It is contemplated that the human cream composition may comprise about 18% to about 30% or more fat (i.e., lipids). In one embodiment, the human cream composition is about 25% fat.
• the human cream compositions described herein may comprise one or more additional components in order to have the desired caloric content and/or desired percentage of fat. Accordingly, in one embodiment, the human cream composition comprises added human skim milk permeate. The skim milk permeate
• the human cream composition (“permeate”) is the liquid produced by the ultrafiltration of human skim milk traditionally thought to be a waste product in the manufacture of human milk fortifers.
• the human cream composition comprises deionized (DI) water in addition to high fat human milk.
• the human cream composition is frozen for storage and/or shipment and is thawed prior to use.
• the human cream compositions described herein are produced from whole human milk.
• the human milk may be obtained from an infant's own mother or from one or more donors.
• the human milk is pooled to provide a pool of human milk.
• a pool of human milk comprises milk from two or more (e.g., ten or more) donors.
• a pool of human milk compri ses two or more donations from one donor.
• human milk is provided by donors, and the donors are pre-screened and approved before any milk is processed.
• Various techniques are used to identify and qualify suitable donors.
• a potential donor must obtain a release from her physician and her child's pediatrician as part of the approval process. This helps to insure, inter alia, that the donor is not chronically ill and that her child will not suffer as a result of the donation(s).
• Methods and systems for qualifying and monitoring milk collection and distribution are described, e.g., in U.S. Patent Application No. 12/728,811 (U.S. 2010/0268658), which is incorporated herein by reference in its entirety.
• Donors may or may not be compensated for their donation.
• donor screening includes a comprehensive lifestyle and medical history questionnaire that includes an evaluation of prescription and non-prescription medications, testing for drags of abuse, and testing for certain pathogens.
• the donor may be screened for, e.g., human immunodeficiency virus Type 1 (HIV-1), HIV-2, human T- lyrnphotropic virus Type 1 (HTLV- I), HTLV-I L hepatitis B virus (HBV), hepatitis C virus (HCV), and syphilis.
• HBV-1 human immunodeficiency virus Type 1
• HIV-2 HIV-2
• HTLV- I human T- lyrnphotropic virus Type 1
• HBV hepatitis B virus
• HCV hepatitis C virus
• syphilis e.g., syphilis.
• Donors may be periodically requalified. For example, a donor is required to undergo screening by the protocol used in their initial qualification every four months, if the donor wishes to continue to donate. A. donor who does not requalify or fails qualification is deferred un til such time as they do, or permanently deferred if warranted by the results of requalification screening. In the event of the latter situation, all remaining milk provided by- chat donor is removed from inventory and destroyed or used for research purposes only.
• a donor may donate at a designated facility (e.g., a milk bank office) or, in a preferred embodiment, express milk at home. If the donor will be expressing milk at home, she will measure the temperature in her freezer with, e.g., a supplied theroiometer to confirm that it is cold enough to store human milk in order to be approved.
• donor identity matching may be performed on donated human milk because the milk may be expressed by a donor at her home and not collected at a milk banking facility.
• each donor's milk can be sampled for genetic markers, e.g., DNA markers, to guarantee that the milk is truly from the approved donor.
• genetic markers e.g., DNA markers
• subject identification techniques are known in the art (see, e.g., Internationa] Application Serial No. PCT/US2006/36827, which is incorporated herein by reference in its entirety).
• the milk may be stored (e.g., at -20°C or colder) and quarantined until the test results are received.
• the methods featured herein may include a step for obtaining a biological reference sample from a potential human breast milk donor.
• a biological reference sample may be obtained by methods known in the art such as, but not limited to, a cheek swab sample of ceils, or a drawn blood sample, milk, saliva, hair roots, or other convenient tissue.
• Samples of reference donor nucleic acids e.g., genomic DNA
• samples of reference donor nucleic acids can be isolated from any convenient biological sample including, but not limited to, milk, saliva, buccal cells, hair roots, blood, and any other suitable cell or tissue sample with intact interphase nuclei or metaphase cells. The sample is labeled with a unique reference number.
• the sample can be analyzed at or around the time of obtaining the sample for one or more markers that can identify the potenti al donor.
• Results of the analysis can be stored, e.g., on a computer-readable medium.
• the sample can be stored and analyzed for identifying markers at a later time.
• the biological reference sample may be DNA typed by methods known in the art such as STR. analysis of STR loci, FILA analysis of FILA loci or multiple gene analysis of individual genes/aileles.
• the DNA-type profile of the reference sample is recorded and stored, e.g., on a computer-readable medium.
• the biological reference sample may be tested for self-antigens using antibodies known in the art or other methods to determine a self- antigen profile.
• the antigen (or another peptide) profile can be recorded and stored, e.g., on a computer-readable medium.
• a test sample of human mi lk is taken for identification of one or more identity markers.
• the sample of the donated human milk is analyzed for the same marker or markers as the donor's reference sample.
• the marker profiles of the reference biological sample and of the donated milk are compared.
• the match between the markers (and lack of any additional unmatched markers) would indicate that the donated milk comes from the same individual as the one who donated the reference sample.
• Lack of a match (or presence of additional unmatched markers) would indicate that the donated milk either comes from a non-tested donor or has been contaminated with fluid from a non-tested donor,
• the donated human milk sample and the donated reference biological sample can be tested for more than one marker.
• each sample can be tested for multiple DNA markers and/or peptide markers. Both samples, however, need to be tested for at least some of the same markers in order to compare the markers from each sample.
• the reference sample and the donated human milk sample may be tested for the presence of differing identity marker profiles. If there are no identity marker profiles other than the identity marker profile from the expected subject, it generally indicates that there was no fluid (e.g., milk) from other humans or animals contaminating the donated human milk. If there are signals other than the expected signal for that subject, the results are indicative of contamination. Such contamination will result in the milk failing the testing.
• identity marker profiles other than the identity marker profile from the expected subject
• the testing of the reference sample and of the donated human milk can be carried out at the donation facility and/or milk processing facility.
• the results of the reference sample tests can be stored and compared against any future donations by the same donor.
• the milk is then tested for pathogens.
• the milk may be genetically screened, e.g., by polymerase chain reaction (PCR), to identify, e.g., viruses, such as HTV-1, HBV and HCV.
• PCR polymerase chain reaction
• a microorganism panel that screens for various bacterial species, fungus and mold via culture may also be used to detect contaminants.
• a microorganism panel may test for aerobic count, Bacillius cerens, Escherichia coli, Salmonella, Pse domonas, conforms, Staphylococcus aureus, yeast and mold.
• B. cereus is a pathogenic bacterium that cannot be removed through pasteurization. Pathogen screening may be performed both before and after pasteurization,
• the donor milk may also be tested for drugs of abuse (e.g., cocaine, opiates, synthetic opioids (e.g. oxycodone/oxymorphone) methamphetamines, benzodiazepine, amphetamines, and THC) and/or adulterants such as non-human proteins.
• drugs of abuse e.g., cocaine, opiates, synthetic opioids (e.g. oxycodone/oxymorphone) methamphetamines, benzodiazepine, amphetamines, and THC
• an ELISA may be used to test the milk for a non-human protein, such as bovine proteins, to ensure, e.g., that cow milk or cow milk infant formula has not been added to the human mil k, for example to increase donation volume when donors are compensated for donations.
• the human milk is processed to produce a high fat product, e.g., a human cream composition.
• the donation facility and milk processing facility can be the same or different facility. Processing of milk can be earned out with large volumes of human milk, e.g., about 75 liters/lot to about 2,000 liters/lot of starting material .
• Methods of obtaining compositions that include lipids from human milk to provide nutrition to patients are described in PCX Application PCT/US07/86973 filed on December 10, 2007 (WO 2008/073888), the contents of which are incorporated herein in their entirety,
• the milk After the human milk is carefully analyzed for both identification purposes and to avoid contamination as described above, the milk then undergoes filtering, e.g., through about a 200 micron filter, and heat treatment. For example, the composition can be treated at about 63°C or greater for about 30 minutes or more.
• the milk is transferred to a separator, e.g., a centrifuge, to separate the cream (i.e., the fat portion) from the skim.
• the skim can be transferred into a second processing tank where it remains at about 2 to 8°C until a filtration step.
• the cream separated from the skim can undergo separation again to remove more skim.
• the skim portion undergoes further filtration, e.g., ultrafiltration. This process concentrates the nutrients in the skim milk by filtering out the water. The water obtained during the concentration is referred to as the permeate.
• the resulting skim portion can be further processed to produce human milk fortifiers and/or standardized human milk formulations.
• PROLACTPLUSTM Human Milk Fortifiers e.g., PROLACT+4TM, PROLACT+6TM,
• PROLACT+8TM, and/or PRQLACT- ⁇ 10TM which are produced from human milk and contain various concentrations of nutritional components
• compositions of the fortifiers are described in U.S. Patent Application Serial No. 1 1/947,580, filed on November 29, 2007, (U.S. 2008/0124430) the contents of which are incorporated herein in their entirety.
• These fortifiers can be added to the milk of a nursing mother to enhance the nutritional content of the milk for, e.g., a preterm infant,
• PROLACT2QTM, NEO20TM, and/or PROLACT24 are also discussed in U.S. Patent Application Serial No. 1 1/947,580, filed on November 29, 2007,
• the caloric content of the cream portion is measured.
• a volume of the permeate from the ultrafiltration of the skim portion may be added to the cream portion, thereby providing a formulated human cream composition that has the desired caloric content.
• deionized water may be added to the cream portion in order to provide the formulated human cream composition.
• the desired caloric content of the human cream composition is about 2,0 kcal to about 3,0 kcal or more per ml.
• the desired caloric content is about 2.5 kcai/ml.
• the desired percentage of fat of the human cream composition is about 20% to about 30% or more lipids. In certain embodiments, the desired percentage of fat is about 25% lipids.
• the cream composition undergoes pasteurization.
• the composition can be placed in a process tank that is connected to the high-temperature, short-time (HTST) pasteurizer via platinum-cured silastic tubing.
• HTST high-temperature, short-time
• the cream composition can be collected into a second process tank and cooled.
• Other methods of pasteurization known in the art can be used.
• the cream composition in the tank is heated to a minimum, of 63°C and h eld at that temperature for a mmimum. of thirty minutes.
• the air above the cream composition is steam heated to at least three degrees Celsius above the cream composition temperature.
• the product temperature is about 66°C or greater
• the air temperature above the product is about 69°C or greater
• the product is pasteurized for about 30 minutes or longer.
• both. HTST and vat pasteurization are performed.
• the pasteurized cream composition is generally processed aseptically. After cooling to about 2 to 8°C, the product is filled into containers of desired volumes, and various samples of the cream composition are taken for nutritional and bioburden analysis.
• the nutritional analysi s ensures proper calorie and fat content of th e cream composition.
• a label that reflects the nutritional analysis is generated for each container.
• the bioburden analysis tests for presence of contaminants, e.g., total aerobic count, B. cereus, E. coli, Conform, Pseudomonas, Salmonella, Staphylococcus, yeast, and/or mold. Bioburden testing can be genetic testing.
• the product is packaged and shipped once the analysis is complete and desired results are obtained.
• the resultant human cream composition comprises about
• the human cream composition comprises about 2.5 kcai/ml. it is contemplated that the resultant human cream composition comprises about 20% to about 30% or more fat. In one embodiment, the human cream composition is about 25% fat.
• the human cream compositions described herein may be used as supplemental nutrition. Accordingly, the human cream compositions described herein may be administered enteraliy or orally (e.g., bottle feeding).
• enteraliy or orally e.g., bottle feeding.
• the use of human lipids for parenteral nutrition, a practice of intravenous feeding (e.g., total parenteral nutrition), for a patient in need thereof is described in PCT Application PCT/US07/86973 filed on December 10, 2007 (WO
• the disclosed human cream compositions are particularly useful for supplementing human milk for infants, especially LBW infants, in order to raise the caloric content of the human milk to a desired level.
• Human milk is often administered enteraliy to preterm infants in the NICU. Enteral nutrition is a practice of tube feeding, e.g., nasogastric, transpyloric, and percutaneous.
• Human milk e.g., mother's own or donor
• Human milk often does not meet the caloric requirements of a LBW infant (Wocjik et al.
• the human cream composition of the current invention is added to the human milk, thereby increasing the caloric content while also maintaining the entirely human milk diet of the infant and avoiding the complications associated wit TPN.
• the enteral nutrition comprising the human cream composition is for a preterm or LBW infant.
• a method of increasing the caloric content of human milk to a desired caloric content level comprises the steps of obtaining a sample of human milk (e.g., mother's own or donor or pool of mil k derived from the mother and/or donors), measuring the caloric content of the human milk, determining a volume of a human milk cream composition needed to raise the caloric content of the human milk to the desired caloric content level, and adding the volume of the human milk cream composition to the container of human milk.
• the desired caloric content is 20 kcal/oz or more.
• the desired calorie target is 24 kcal/oz or more.
• the desired calorie target is 26 kcal/oz or more.
• the human milk cream composition used to increase the caloric content of the human milk may comprise, e.g., about 2.5 kcai/ml and/or about 25% fat.
• a human cream composition was produced that can be delivered enteral iy, thereby avoiding the negative effects associated with TPN.
• Human milk from previously screened and approved donors was mixed together to generate a pool of donor milk.
• the pool of donor milk was further tested for specific pathogens and bovine proteins. Specifically, PGR testing was used to screen for the presence of HIV- 1, HBV, and HCV in the milk.
• a microbiological panel was also performed that tests for, e.g., aerobic count, Bacillius cereus, Escherichia coli,
• Salmonella Pseudomonas, coiiforms, Staphylococcus aureus, yeast and mold.
• the pool of donor milk was ultracentrifuged to generate a cream portion and a skim milk portion.
• the cream portion was then formulated to meet specific fat and calorie specifications by adding an amount of the water ultra-filtered from the skim portion, the human skim milk ultrafiltration permeate. Specifically, the cream portion was standardized to 25% lipids and contained about 2.5 kcai/ml.
• the standardized cream composition was then pasteurized following guidance set by the FDA's Pasteurized Milk Ordinance. Following pasteurization, the standardized cream composition was then filled into high density polyethylene bottles and frozen. The bottles were weighed to ensure that the intended volume was filled into the bottle. The bottled cream composition was then quarantined until all data from the microbiological panel was reviewed and a full nutritional analysis was performed.
• the bottled cream composition was labeled with a lot specific "use by" date and product lot number.
• the cream product was then shipped frozen to the destination, e.g., hospital, in an insulated cooler packed with dry ice.
• infants were randomized using a stratified (by study site) block scheme into either the test group that received the human cream supplement or the control group that did not receive the human cream supplement. All other nutrition (e.g., human milk and Prolact+ H 2 MF*) was provided in the same fashion and according to institutional standard practice for both study groups, [0070] The randomization was performed in blocks of 4 without the use of stratification variables except for study site because of the small study size. Block size was kept blinded. While blinding of study groups is always desirable in randomized studies, because of the nature of the interventions, e.g., the need to test the human milk in only one group and the methods by which the nutrition was prepared and delivered, it was not possible for this study.
• All other nutrition e.g., human milk and Prolact+ H 2 MF*
• the study required 31 subjects per group (62 total) in order to demonstrate the lack of inferiority hypothesis.
• their human milk e.g., mother's or donor
• this might occur as much as 25% of the time.
• the sample size for the cream test group was increased by about 25% to 39 subjects, and the control group was also increased to keep the study balanced.
• the resulting total sample size was 78 subjects.
• the cream test group was an intent-to-treat (ITT) group in spite of the fact that some of the infants may not receive the cream supplement.
• the amount to be added was calculated to the nearest ml by rounding down for 0.1 -0.4 ml and rounding up for 0.5-0.9 mi in order to avoid imprecision due to the calorie measuring device used in the nutrition preparation area.
• an equal volume of human miik w r Prior to adding the calculated volume of the cream composition, an equal volume of human miik w r as removed from the container, thereby maintaining the same total volume.
• the calorie-adjusted, if required, human milk and subsequent Prolactr H 2 MF ⁇ was then provided according to institutional standard care.
• the infants randomized into the control group were provided the human milk and Prolact+ EPMF"' according to the institutional standard of care without any use of the milk analysis, as is typical for the vast majority of neonatal intensive care units.
• TTN transient tachypnea of newborn
• ⁇ intraventricular hemorrhage
• PDA patent ductus arteriosus
• Head circumference increment for the study period and length increment for the study period were determined. Length and head circumference were measured on a weekly basis during the study period and recorded as cm/week. The use of medications that influence growth and development, including postnatal steroids (e.g., hydrocortisone and dexamethasone), caffeine and other methylxanthine preparations were also recorded.
• postnatal steroids e.g., hydrocortisone and dexamethasone
• caffeine and other methylxanthine preparations were also recorded.
• the daily amount of all enteral nutrition was recorded in units of ml. This quantity was subdivided into the various types of nutrition used, i.e., mother's milk, donor milk, cream supplement, Prolaet+ I I ' VIF ⁇ and any other nutritional support, in the event of feeding intolerance or discontinuation of enteral feeding and the infant returning to total parenteral nutrition (TPN), the assumed cause was identified. In addition, the total number of days of TPN was recorded for the study period.
• fable 2 shows the growth velocities defined from birth and from the time to regain birth weight

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Food Science & Technology (AREA)
• Polymers & Plastics (AREA)
• Health & Medical Sciences (AREA)
• Nutrition Science (AREA)
• Dairy Products (AREA)
• Medicines Containing Material From Animals Or Micro-Organisms (AREA)
• Coloring Foods And Improving Nutritive Qualities (AREA)
• Medical Treatment And Welfare Office Work (AREA)
• General Preparation And Processing Of Foods (AREA)

Priority Applications (16)

Applications Claiming Priority (2)

Publications (1)

Family

ID=51528165

Family Applications (1)

Country Status (15)

Cited By (3)

Families Citing this family (4)

Citations (6)

Family Cites Families (100)

• 2014
  • 2014-03-05 CA CA2902990A patent/CA2902990C/en active Active
  • 2014-03-05 WO PCT/US2014/020837 patent/WO2014158911A1/en not_active Ceased
  • 2014-03-05 EP EP14774486.6A patent/EP2967094B1/en active Active
  • 2014-03-05 DK DK14774486.6T patent/DK2967094T3/da active
  • 2014-03-05 FI FIEP20216091.7T patent/FI3821713T3/fi active
  • 2014-03-05 DK DK20216091.7T patent/DK3821713T3/da active
  • 2014-03-05 ES ES20216091T patent/ES3015561T3/es active Active
  • 2014-03-05 PL PL14774486T patent/PL2967094T3/pl unknown
  • 2014-03-05 JP JP2016500678A patent/JP6853665B2/ja active Active
  • 2014-03-05 CN CN201480025425.6A patent/CN105246342A/zh active Pending
  • 2014-03-05 PT PT202160917T patent/PT3821713T/pt unknown
  • 2014-03-05 MX MX2015012205A patent/MX386497B/es unknown
  • 2014-03-05 PL PL20216091.7T patent/PL3821713T3/pl unknown
  • 2014-03-05 PT PT147744866T patent/PT2967094T/pt unknown
  • 2014-03-05 CN CN202210553069.1A patent/CN114794241A/zh active Pending
  • 2014-03-05 BR BR112015022059-2A patent/BR112015022059B1/pt active IP Right Grant
  • 2014-03-05 ES ES14774486T patent/ES2843254T3/es active Active
  • 2014-03-05 EP EP20216091.7A patent/EP3821713B1/en active Active
  • 2014-03-05 US US14/198,432 patent/US11122813B2/en active Active
  • 2014-03-05 EP EP24219978.4A patent/EP4520179A3/en active Pending
  • 2014-03-05 AU AU2014241754A patent/AU2014241754B2/en active Active
• 2015
  • 2015-08-17 IL IL240601A patent/IL240601B/en active IP Right Grant
• 2018
  • 2018-02-28 AU AU2018201444A patent/AU2018201444B2/en active Active
• 2019
  • 2019-05-15 US US16/412,699 patent/US11419342B2/en active Active
  • 2019-09-25 JP JP2019174723A patent/JP2020043854A/ja active Pending
• 2022
  • 2022-07-13 US US17/863,999 patent/US12063943B2/en active Active
• 2024
  • 2024-06-28 US US18/759,044 patent/US20250017228A1/en active Pending

Patent Citations (7)

Non-Patent Citations (7)

Also Published As

Similar Documents

Legal Events