WO2014148562A1 - がん幹細胞を含む細胞集団を得る方法 - Google Patents
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Definitions
- the present invention relates to a method for obtaining a cell population containing cancer stem cells.
- cancer is a genetic disease
- the “carcinogenic multi-stage theory” is basically that four or more genes are continuously mutated over time and the effects of the mutation accumulate.
- each is considered as a uniform tissue consisting of a single cancer cell.
- individual cancer therapeutics especially those that are called molecular target cancer therapeutics, are of a single or limited type directed to a “molecular target” such as a specifically expressed protein.
- Biomarkers that attack cancer cells such as proteins expressed on the surface of cancer cells or “tumor markers” that are released by release from cancer cells into body fluids. It is standard that specific sex is diagnosed and therapeutic drugs and treatment methods are determined.
- cancer stem cell theory The involvement of cancer stem cells has been proposed as a cause of intractable cancer (cancer stem cell theory). Cancer stem cells have been isolated and reported from various cancers (patient tissues), but their low presence and difficulty in maintaining stable culture make it difficult to isolate and purify. This hinders research and analysis. Not all cancer stem cells have been isolated. The type of cancer cells in the tumor tissue varies from patient to patient, is heterogeneous, and is also considered limited. For this reason, it may not be easy to grasp all types of cancer cells, so researchers who express negative opinions about the existence of cancer stem cells themselves or the role they play in the progression and progression of cancer There are also many. Patent Document 1 (included herein by reference) discloses a screening method for a cancer-killing stem cell substance using cancer stem cells.
- cancer treatment with conventional cancer drugs problems such as cancer recurrence and metastasis are always present, and the therapeutic effect is expressed as “5-year survival rate”, etc., and the overall survival (OS) or life-prolonging effect Is the end point (indicator).
- OS overall survival
- life-prolonging effect Is the end point (indicator).
- cancer drugs including molecular targeted therapies, are effective only on certain cancer cells in the population, and the remaining cancer cells and cancer stem cells that are heterogeneous. Therefore, it is speculated that the treatment action will help the growth of these remaining cancer cells and cancer stem cells, leading to recurrence and metastasis, so that the cancer is rarely “cured”.
- understanding and countermeasures for "heterogeneity" of cancer cells are required.
- Non-patent Document 1 Non-patent Document 1 (included in this specification by reference), Diamond CD133 Isolation Kit manufactured by Miltenyi Biotec) are used as a marker protein such as CD133 and CD44 as an index.
- a separation method and a separation and recovery method by flow cytometry Non-Patent Document 2 (included in this specification by reference)), laser microdissection (Leica's LMD6500 and LMD7000), and the like.
- Non-patent Document 3 (included herein by reference), Sigma-Aldrich) Hystem manufactured by the company, collagen gel (Matrigel manufactured by Nippon Becton Dickinson Co., Ltd.) and the like can be used.
- Conventional techniques for isolating CD44-expressing cells require expensive equipment and reagents, and when equipment is used, space for installation and specialized techniques are required.
- the present invention is to induce cancer stem cells for the first time by culturing iPS cells, and cancer cells that differentiate from the induced cancer stem cells may coexist. can do.
- the present invention was completed by finding that, when hyaluronic acid was added to the medium in order to concentrate cancer stem cells, the concentration of CD44 highly expressing cells became unexpectedly efficient. Specifically, although illustrated below, the present invention is not limited to these. 1.
- the method according to 8 above further comprising selecting a candidate substance that inhibits the proliferation of the cell population or cells. 10. 10. The method according to 8 or 9 above, wherein the cell population containing the cancer stem cell according to 5 is contacted with a candidate substance for a cancer therapeutic agent. 11. A method for concentrating CD44-expressing cells, comprising culturing a cell population containing CD44-expressing cells in a non-adherent culture in the presence of hyaluronic acid. 12 12. The method according to 11 above, wherein the cell population is derived from human. 13. The method according to 11 or 12, wherein the culture is performed for 2 to 20 days. 14 A cell population in which CD44-expressing cells are enriched by the method according to any one of 11 to 13 above.
- the present invention it is possible to obtain a cell population containing various cancer stem cells that constitute a tumor in vivo and various cancer stem cells.
- Tumor formation by miPS -LLCcm cells Histological evaluation of tumors formed from miPS -LLCcm cells. Explanatory drawing of the immuno-staining result of the tumor formed from the miPS * -LLCcm cell.
- Cells lumened in Matrigel®.
- the suspension culture shows spheroids obtained by culturing miPS -LLCcm cells in a serum-free medium. The left photograph shows the expression of GFP.
- Immunostaining of tumors formed from spheroids of miPS ⁇ -LLCcm cells Left) Expression of p53, a typical tumor suppressor gene. Right) Expression of matrix metalloproteinase 2 (MMP2) involved in cancer invasion and metastasis. Expression of stem cell markers. Visualization of mouse cancer iPS-CSC gene expression profile by spherical self-organizing mapping. In the figure, in the second and third cells from the upper left and the first and second cells from the lower left, mainly red (gene highly expressed compared to iPS cells) and blue ( Genes whose expression levels are low compared to iPS cells) are displayed.
- MMP2 matrix metalloproteinase 2
- iPS cells are cells derived from vertebrates, preferably mammals. Mammals include cells derived from humans, mice, rats, hamsters, rabbits, dogs, cats, cows, horses, sheep, monkeys. iPS cells are preferably derived from primates or rodents, more preferably from humans or mice, and even more preferably from humans.
- Cancer cells are cells derived from vertebrates, preferably mammals. Mammals include cells derived from humans, mice, rats, hamsters, rabbits, dogs, cats, cows, horses, sheep, monkeys. The cancer cells are preferably derived from primates or rodents, more preferably from humans or mice, and even more preferably from humans. As cancer cells, lung cancer cells, embryonal carcinoma cells, malignant melanoma, breast cancer cells, colon cancer cells, pancreatic cancer cells, brain tumor cells, liver cancer cells, hepatocellular carcinoma cells, digestive cancer cells And ovarian cancer cells, leukemia cells, lymphoma cells and the like.
- cancer cells LLC, P19-CL6, B16-F10, BALB MC.E12, A172, U251-MG, MDA-MB-415, MDA-MB-231, T-47D, ZR-75-1, SK-BR-3, BT-549, MCF7, HT-29, PANC1, SKOV3, HepG2, Huh-7, PLC / PRF-5, ECC, CW-2, PMF-ko14, MY, MOLT4, KLM-1, Cells such as PK8, PK59, A549, Li-7, OVCAR-3, Lu99B, RERF-LC-KJ, OVK18, and RERF-LC-AI can be used.
- PK8 PK59, A549, Li-7, OVCAR-3, Lu99B, RERF-LC-KJ, OVK18, and RERF-LC-AI can be used.
- the cancer cells are LLC, P19-CL6, B16-F10, BALB MC.E12, A172, MDA-MB-415, T-47D, BT-549, HT-29, SKOV3, MY, MOLT4, Selected from PK59, A549, Li-7, OVCAR-3, Lu99B, and RERF-LC-KJ.
- the cancer cells are A172, MDA-MB-415, T-47D, BT-549, HT-29, SKOV3, MY, MOLT4, PK59, A549, Li-7, OVCAR-3, Lu99B, RERF -Selected from LC-KJ.
- the origin of cancer cells for obtaining iPS cells and culture supernatant may or may not coincide.
- human iPS cells may be cultured in the presence of a culture supernatant of cancer cells derived from mice, rats, or monkeys.
- the iPS cells and the cancer cells from which the culture supernatant is obtained have the same origin.
- any cell culture medium selected according to the cancer cells to be used can be used.
- DMEM Glasgow MEM
- EMEM EMEM
- MEM ⁇ RPMI-1640
- Ham F-10 Medium 199
- Ames' Medium BGJb Medium
- EHAA TMRL-1066
- Fischer's Medium IMDM, Leibovitz
- McCoy's 5A Modified Medium NCTC Medium, Swim's Wayum Medium, William's Medium E, Ham F-12, MCDB medium, and the like, but are not limited thereto.
- the culture supernatant of cancer cells can be obtained, for example, by washing cancer cells that have reached 80% confluence in an incubator with a new medium, and further in a new medium for 24 to 72 hours, preferably 24 to 48 hours. Continue culturing and obtain.
- the medium for obtaining the culture supernatant may or may not contain serum.
- the culture supernatant may be used after passing through a 0.45 ⁇ m pore or 0.22 ⁇ m pore filter. Because the liquid fraction of the cancer cell culture supernatant contains a factor that induces cancer stem cells, macromolecules such as cell fragments and exosomes were removed from the cancer cell culture supernatant.
- the liquid fraction may be used as a “cancer cell culture supernatant” in the method of the present invention.
- any medium for culturing stem cells can be used, for example, DMEM, Repro FF2 or Repro stem (Reprocell), NON-ESSENTIAL AMINO ACID, 200 mM L-GLUTAMINE, Examples include, but are not limited to, KSR and DMEM-F12 medium containing 0.1M 2-MERCAPTOETHANOL / PBS.
- IPS cells are cultured in the presence of a culture supernatant of cancer cells for about 2 weeks to 2 months, preferably about 4 weeks to 2 months.
- a part (for example, half) or the whole of the iPS cell culture medium may be replaced with the culture supernatant of cancer cells once every 1 to 4 days.
- the presence of cancer stem cells in the obtained cell population indicates the ability to form malignant tumors in nude mice, specifically pathological diagnosis after specimen preparation (active cell division, atypical cells, presence of angiogenesis, etc.) Can be confirmed. It can also be confirmed by evaluating the ability to form blood vessel structures in vitro, gene expression fluctuations of tumor suppressors represented by p53, expression of known cancer stem cell markers such as CD44, and the like.
- Cancer stem cells are cells having differentiation ability, and finally reach cancer cells that do not differentiate any more. Cancer stem cells are cells located between iPS cells and cancer cells, and there are various cancer stem cells with different degrees of differentiation.
- the cell population obtained by the method of the present invention is a heterogeneous cell population containing various cancer stem cells, and may contain cancer cells.
- the cell population obtained by the method of the present invention can be separated into cancer stem cells having different levels of differentiation by a cell sorter or the like, or can be separated into cells one by one according to a known technique.
- cancer stem cells are thought to form spheroids by self-replication of single cells, single cell cloning by spheroid culture can be performed and separated into individual cells.
- a cell population containing various cancer stem cells constituting a tumor in vivo can be created in vitro from pluripotent iPS cells.
- the cell population obtained by the present invention is sufficiently short to be induced from iPS cells, so it is considered that no gene mutation has been introduced. That is, according to the present invention, it is possible to comprehensively induce many types of cancer stem cells without accumulation of gene mutations.
- the cell population and cancer stem cells obtained by the present invention it is possible to comprehensively and systematically analyze and study cancer cells and cancer stem cells generated in the living body. For example, comprehensive gene expression analysis, epigenetic analysis, proteome analysis, and metabolome analysis using a next-generation sequencer can be performed to create a database as cell information, which can be used for the development of cancer therapeutics.
- the cell population or cancer stem cells obtained by the method of the present invention can be used in a method for screening cancer drugs.
- Examples of the method for bringing the cell population or cancer stem cells obtained by the method of the present invention into contact with a candidate substance for a cancer therapeutic agent include the cell population obtained by the method of the present invention or The method of adding to the culture medium of a stem cell is mentioned.
- Candidate substances are, for example, organic low molecular weight compounds, nucleic acids, carbohydrates, lipids, amino acids, proteins, antibodies, compound libraries prepared using combinatorial chemistry techniques, random peptide libraries prepared by solid-phase synthesis or phage display methods , Natural components (components derived from microorganisms, animals and plants, marine organisms, etc.).
- concentration of the candidate substance in the medium include about 1 ppb to 1%, preferably about 1 ppm to 0.1%. If the candidate substance is difficult to dissolve in water, it can be dissolved in an appropriate organic solvent such as ethanol or DMSO, or dissolved in the medium as a salt of the candidate substance and a non-toxic acid or base.
- the action of the candidate substance can be evaluated using, for example, cell proliferation as an index.
- a cell population containing cancer stem cells obtained by the method of the present invention or a candidate substance that inhibits cell proliferation of cancer stem cells is selected as a substance useful as a cancer therapeutic agent.
- a method for evaluating cell proliferation a method of counting the number of living cells with a microscope, an MTT assay method, a trypan blue dye exclusion test method, an intracellular ATP measurement method, a resazurin metabolic activity test method (QBlue assay method), an AlamarBlue assay method Etc.
- a candidate substance that inhibits cell proliferation by 10% or more, preferably 20% or more, more preferably 50% or more compared with the control is selected as a substance useful as a cancer therapeutic agent.
- CD44 is a single-transmembrane glycoprotein hyaluronic acid receptor that is specifically highly expressed on the surface of cancer cells such as glioma, and is used in human pancreatic cancer, prostate cancer, breast cancer, etc. It is known to be a cancer stem cell marker. CD44 has functions such as cell aggregation, maintenance of matrix around cells, matrix-cell signaling, and matrix metalloprotease (MMP) orientation control (cell migration, invasion).
- MMP matrix metalloprotease
- the cell population containing CD44-expressing cells is a cell population derived from vertebrates, preferably mammals. Mammals include cells derived from humans, mice, rats, hamsters, rabbits, dogs, cats, cows, horses, sheep, monkeys.
- the cell population containing CD44-expressing cells is preferably derived from primates or rodents, more preferably from humans or mice, and even more preferably from humans.
- the cell population containing CD44-expressing cells preferably contains CD44 high-expressing cells that can detect CD44 at the protein level, such as Western blotting or immunostaining.
- the CD44-expressing cell is a cancer stem cell.
- Cell populations containing CD44-expressing cells include U251MG cells and A172 cells (human glioma), SkOv-3 cells (human ovarian cancer), 4T1 cells (mouse breast cancer), MDA-MB-231 cells (human breast cancer), AsPC- One cell (human pancreatic cancer), PC-3 cell (human prostate cancer) and the like can be used.
- Hyaluronic acid is composed of disaccharide repeating units of N-acetylglucosamine and glucuronic acid.
- the molecular weight of hyaluronic acid used in the present invention is about 500 to 20 million, preferably about 1000 to 1500,000, more preferably about 3000 to 1000000.
- natural hyaluronic acid may be used as it is, or low molecular weight hyaluronic acid having a molecular weight reduced by degradation with an enzyme (hyaluronidase) or hydrolysis with an acid may be used.
- hyaluronic acid includes both high-molecular hyaluronic acid and low-molecular hyaluronic acid.
- Polymer hyaluronic acid refers to hyaluronic acid having a molecular weight of 100,000 or more, preferably 300,000 or more, more preferably 500,000 or more, still more preferably 700,000 or more, and particularly 1,000,000 or more.
- Hyaluronic acid obtained from nature corresponds to these “hyaluronic acids” having a large molecular weight (high molecular weight).
- hyaluronic acid having a molecular weight of a certain level or less is separated from hyaluronic acid having a low molecular weight or naturally-derived hyaluronic acid.
- the “low molecular hyaluronic acid” to be used can be obtained.
- Low molecular hyaluronic acid refers to hyaluronic acid having a molecular weight of 100,000 or less, the upper limit of molecular weight is about 100,000, 70,000, 30,000, 10,000, 10,000, and the lower limit of molecular weight is 500, It is about 1000, 2000, 3000.
- Hyaluronic acid may be derived from an animal such as a chicken's tail or umbilical cord, or may be derived from a microorganism such as lactic acid bacteria or streptococci.
- Hyaluronic acid has a concentration in the medium of about 0.1 ⁇ g / ml to 5000 ⁇ g / ml, preferably about 1 ⁇ g / ml to 3000 ⁇ g / ml, more preferably about 5 ⁇ g / ml to 1000 ⁇ g / ml, and even more preferably 10 ⁇ g / ml. It is added so as to be about ml to 200 ⁇ g / ml.
- a culture method and culture conditions for a cell population containing CD44-expressing cells general culture methods and culture conditions corresponding to each cell type can be employed.
- a medium to which hyaluronic acid is added any cell culture medium can be used, and examples thereof include DMEM, Glasgow MEM (GMEM), EMEM, MEM ⁇ , RPMI-1640, Ham F-12, MCDB medium and the like. However, it is not limited to these.
- antibiotics such as penicillin and streptomycin, serum, various growth factors or differentiation-inducing factors may be added to these media.
- the culture container may be any culture container or culture dish such as a flask, petri dish, petri dish, plate, tissue culture tube, tray, culture bag, roller bottle, or hollow fiber suitable for non-adhesive culture.
- the culture vessel is a culture vessel having a non-cell-adherent inner bottom surface. Cultured cells may or may not adhere to the non-cell-adherent inner bottom surface to such an extent that spheroid (cell mass) formation is not inhibited.
- the culture container having a cell non-adhesive inner bottom surface may be any known or commercially available cell non-adhesive culture container, for example, a special surface treatment is performed by injection molding a general-purpose plastic such as polystyrene.
- Highly hydrophilic such as non-culture containers, non-woven fabric or porous film scaffolds, plastic molded culture containers with fine irregular surfaces, polyethylene glycol, polyhydroxyethyl methacrylate, ethylene vinyl alcohol copolymer
- surface treatment such as plasma treatment
- the number of cultured cells is appropriately determined in consideration of the capacity of the culture vessel, the cell type, the final spheroid size, etc. For example, 10 4 to 10 7 cells, preferably 10 4 cells per 1 mL of medium. Seed in culture vessel at ⁇ 10 6 final concentration.
- the culture may be shake culture or static culture.
- the exchange of the culture solution is not particularly limited as long as spheroids are formed, but may be exchanged once or twice a day, for example, or once every 2 to 4 days. It is also possible to perform a treatment such as centrifugation before exchanging the culture solution to precipitate the cells and then exchange the culture solution.
- the culture period is about 2 to 20 days, preferably about 2 to 14 days, more preferably about 5 to 14 days.
- the culture temperature is preferably around 37 ° C.
- CD44-expressing cells By culturing a cell population containing CD44-expressing cells in the presence of hyaluronic acid under non-adherent culture conditions, spheroids of CD44-expressing cells are formed, and CD44-expressing cells are concentrated.
- the expression level of CD44 is increased, cell proliferation is further promoted, and cell functions are also enhanced.
- the concentrated CD44-expressing cells can be isolated and purified by a known method.
- the size of the spheroid is usually about 10 to 500 ⁇ m in diameter, preferably about 50 to 200 ⁇ m. If the spheroids become too large, nutrients will not easily penetrate into the spheroids. Therefore, it is desirable to separate the spheroids when they reach an appropriate size.
- the obtained spheroids can form spheroids repeatedly by separating cells and then performing non-adherent culture again.
- CD44-expressing cells can be enriched by the formation of spheroids, and by purifying spheroid formation, more pure (homogeneous trait) CD44-expressing cells can be obtained.
- Example 1 Induction of cancer stem cells from mouse iPS cells (1) Culture of mouse iPS cells Mouse iPS cells (iPS-MEF-Ng-20D17, purchased from Riken Cell Bank) are in DMEM medium (15% FCS, 0.1 mM NEAA, 2 mM L- Glutamine, 0.1 mM 2-mercaptoethanol, 1000 U / ml LIF, 50 U / ml penicillin, and 50 U / ml streptomycin) were used and maintained on MEF cells at 37 ° C. and 5% CO 2 . The mouse iPS cells used in this experiment stably express the GFP gene in an undifferentiated state and disappear when differentiated (Okita, K. et al. Nature 448: 313-317 (2007); include).
- Mouse Lewis lung cancer cell line LLC cells were cultured in DMEM medium containing 10% serum.
- Mouse embryonal carcinoma cell P19-CL6 cells were cultured in ⁇ MEM medium (containing 10% FCS, 50 U / ml penicillin, 50 U / ml streptomycin) containing 10% serum.
- Mice malignant melanoma cells B16-F10 cells, and breast cancer cells BALB MC.E12 cells MEM medium containing 10% serum (10% FCS, 50 U / ml penicillin, 50 U / ml streptomycin) at 37 °C, 5% CO 2 Cultured under. Each cell was cultured until confluent, and the culture supernatant was collected and filtered using a 0.45 ⁇ m filter to obtain a conditioned medium.
- mice iPS cells are collected by trypsin treatment, and 7 ⁇ 10 5 cells are collected in DMEM medium (15% FCS, 0.1 mM NEAA, 2 mM L-glutamine, 0.1 mM 2-mercaptoethanol, 50 U / Inoculated into a new 60 mm diameter gelatin-coated dish using ml penicillin and 50 U / ml streptomycin), and after 8 hours, an equivalent conditioned medium was added and the culture was continued. The main culture was continued for 4 weeks.
- the induced cells were maintained in DMEM medium (containing 15% FCS, 0.1 mM NEAA, 2 mM L-glutamine, 0.1 mM 2-mercaptoethanol, 50 U / ml penicillin, 50 U / ml streptomycin).
- Tumorigenicity evaluation Tumor-forming ability of cancer stem cells induced from iPS cells was evaluated using nude mice (Balb / c Slc-nu / nu 6-8 week-old females) (FIG. 1). 4 ⁇ 10 6 miPS-LLCcm cells were suspended in 100 ⁇ l of DMEM medium (containing 10% serum) and transplanted by subcutaneous injection on the back of nude mice. After 4 weeks, the formed tumor was excised, fixed with 10% neutral formalin buffer (Wako Pure Chemical Industries) for 24 hours, then embedded in paraffin, a 3 ⁇ m thick section was prepared, and hematoxylin / eosin staining was performed. went. The same experiment was performed for other cells.
- FIG. 2 shows the histological analysis results of a tumor formed by transplanting miPS-LLCcm cells. On the other hand, teratoma (benign) occurred in iPS cells, and no malignant tumors occurred.
- cm Conditioned medium
- Tumor sections were deparaffinized with xylene, heated in 10 mM citrate buffer (pH 6) at 95 ° C for 5 minutes, and then digested with protease K (37 ° C, 30 minutes) for antigen.
- Anti-GFP antibody (provided by Associate Professor Ayano Sato, Okayama University, 1: 200), anti-cytokeratin antibody (anti-pan-Cytokeratin (AE1 / AE3) antibody, Santa Cruz 1: 200), anti-CD31 antibody (Santa Cruz 1 : 200). Color was developed with a biotinylated secondary antibody (Vector), ABC reagent (Vector), DAB (Vector), and hematoxylin staining was performed as a counterstain.
- Tumors arising from miPS-LLCcm cells include cells that express only GFP (GFP + CK-), cells that express only cytokeratin (GFP- CK +), and cells that do not express both (GFP- CK) -) was found (Fig. 3).
- GFP + cells indicate cells that are still undifferentiated
- CK + cells indicate cells that have differentiated into epithelial cells.
- Cells that are negative for both indicate that they have differentiated into cells in the middle of epithelial differentiation that have not yet expressed cytokeratin, or cells other than the epithelial system (at least do not express cytokeratin). This indicates that the tumor is a heterogeneous population of various cells.
- MiPS-LLCcm cells were observed to form a vascular-like lumen structure.
- cells contained in this structure were stained for the vascular endothelial marker CD31, GFP positive and CD31 positive (GFP +, CD31 +), GFP positive and CD31 negative (GFP +, CD31 ⁇ ), GFP negative and CD31 positive (GFP ⁇ , CD31 +), Both cells were found to be negative (GFP-, CD31-) (Fig. 4).
- the cell population obtained in the present invention is a cell population that has the ability to differentiate into cells involved in angiogenesis and form blood vessels composed of at least four types of cells in vitro. ing.
- spheroid cells Primary culture was performed by a known method from a tumor formed by miPS-LLCcm cells in nude mice to establish miPS-LLCcm primary cells. These cells include GFP positive cells and GFP negative cells (which are considered to be differentiated from GFP positive cells). The cells were separated into single cells by trypsin treatment and seeded on non-surface-treated dishes at 2 ⁇ 10 4 cells / ml (floating culture). As the medium, serum-free DMEM medium (containing 0.1 mM NEAA, 2 mM L-glutamine, 0.1 mM 2-mercaptoethanol, 50 U / ml penicillin, 50 U / ml streptomycin) was used.
- serum-free DMEM medium containing 0.1 mM NEAA, 2 mM L-glutamine, 0.1 mM 2-mercaptoethanol, 50 U / ml penicillin, 50 U / ml streptomycin
- Lung metastasis occurred by injecting the cell population of the present invention into the tail vein of nude mice.
- spheroid cells formed from miPS-LLCcm cells the expression of p53, a tumor suppressor gene, was reduced compared to miPS cells (FIG. 7 left).
- MMP2 matrix metalloproteinase 2
- the expression of matrix metalloproteinase 2 (MMP2) involved in cancer invasion and metastasis was significantly higher in the cancer stem cell line miPS-LLCcm LMT, which was established by primary culture from tumors formed in the lung (Fig. 7 right). ).
- MMP2 matrix metalloproteinase 2
- RNA expression analysis using microarray RNA was extracted from the cell population obtained in this experiment by a method using RNeasy kit (50) (QIAGEN) or a method using TRIzol (Invitorogen). Thereafter, DNase I treatment was performed on 50 ⁇ g or less of RNA, and purification was performed again with RNeasy kit (50) (QIAGEN).
- RNA was reverse transcribed using SuperScriptII (Invitorogen) in the presence of 2 mM oligo dT, 1 mM dATP, 1 mM dCTP, 1 mM dGTP, 0.2 mM dTTP, 0.8 mM aminoalkylated dUTP to synthesize cDNA.
- RNase H treatment was performed.
- the synthesized cDNA was recovered by ethanol precipitation, dissolved in 6.7 ml of 0.1M NaHCO 3 (pH 8.0), added with an equivalent amount (6.7 ⁇ l) of Cy3 dye, and allowed to react at 25 ° C. for 120 minutes in the dark.
- Cy3-labeled cDNA was purified by a QIAquick column (QIAGEN).
- the mouse cell surface protein gene array slide prepared by the inventors using Cy3-labeled cDNA was hybridized at 55 ° C. for 15 hours, and GenePix (registered trademark) Pro5.
- FLA8000 scanner Fluji Film. 1 Fluorescence intensity was detected and analyzed with software (Axon instrument).
- each gene expression level of miPS cells is used as a reference, and the expression level in each cell is clustered on the spherical self-organization map as a relative value, and compared with miPS cells.
- genes with a high rate of change were visualized (FIG. 9).
- FIG. 9 it was found that there are several patterns in the phenotype of the mapping of the cell population including the cancer stem cells to be generated (FIG. 9). This result suggests that even if the same iPS cells are used, cancer stem cells having different functions and properties are generated depending on the conditions used.
- the fluorescence intensity of Cy3 detected by the microarray was normalized, a scatter plot in which the logarithm of the value of the cell to be compared was plotted was created, and the expression patterns of the genes were compared (FIG. 10).
- miPS-MC.E12co a cell population obtained by co-culturing miPS cells with BALB MC.E12 cells
- miPS-LLCcm and miPS-LLCcm LMT miPS-LLCcm cells Changes in gene expression were also observed between the lung metastatic cells (considered as iPS-CSC with high metastatic potential) (FIG. 10 right).
- Example 2 Induction of cancer stem cells from human iPS cells (1) Preparation of cancer cell culture supernatant (conditioned medium) DMEM medium containing 10% FBS, 1% penicillin / streptomycin for the cancer cell lines shown in Table 2 Alternatively, the cells were cultured in an RPMI1460 medium using an adhesion culture dish (diameter: 100 mm, manufactured by TPP) at 37 ° C. and 5% CO 2 .
- the 80% confluent dish medium was removed, washed once with DMEM or RPMI1460 medium containing 5% FBS, and cultured in the same medium.
- the culture supernatant was collected 24 hours after the medium was changed, and centrifuged at 300 ⁇ g for 10 minutes at 4 ° C. Further, the mixture was centrifuged at 2000 ⁇ g for 10 minutes at 4 ° C. and passed through a 0.22 ⁇ m pore filter, and the culture supernatant was used as a conditioned medium.
- OCC-hiPS-1 continued to proliferate while maintaining stem cellity after induction for 28 days, and further, as explained below, it formed tumors in SCID mice, confirming the induction of cancer stem cell transformation .
- OCC-hiPS-3, 5, 8, 10, 12, 19, 20, and 23-28 similarly continued to proliferate while maintaining stemness after 28 days of induction.
- the induction of cancer stem cells was found to be different in derivatization efficiency depending on the conditioned medium (FIG. 12).
- rBC2LCN-FITC® manufactured by Wako Pure Chemical Industries, Ltd.
- Podocalixin detected by rBC2LCN-FITC is a glycoprotein ligand specific for human pluripotent stem cells. Detection was performed according to the protocol of rBC2LCN-FITC. Cells that reacted with rBC2LCN and showed green fluorescence were detected (FIG. 13 right), and were found to have stem cell properties. In addition, green fluorescence was not detected when rBC2LCN was not added (FIG. 14).
- a cell population derived from human iPS cells (OCC-hiPS-1) was suspended in HBSS and transplanted to the testes of Balb / c-nu / nu nude mice or CB17 scid mice. Tumors were excised from OCC-hiPS-1 cell-transplanted mice in which tumor formation was observed in CB17 ⁇ ⁇ ⁇ scid mice, and primary culture was performed (FIG. 15). Part of the tumor was fixed with 4% paraformaldehyde phosphate buffer.
- the primary cell line (OCC-hiPS-29 cells) was cultured on a Laminin-5-coated dish in a differentiation induction medium using A172 cell culture supernatant at 37 ° C. and 2% CO 2 . Passage was performed once every 3-4 days. Stem cell-like cells that react with rBC2LCN-FITC were also detected in OCC-hiPS-29 cells (FIG. 16).
- OCC-hiPS-29 cells were cultured in a differentiation induction medium using A172 cell culture supernatant on a non-adhesive dish for 2 to 5 days. A dense cell part was formed, and a cell sphere was formed in part.
- the cell mass of OCC-hiPS-29 cells was treated with Actase solution (manufactured by SIGMA), then serum-free medium (insulin-transferrin-sodium selenite medium supplement (Sigma-Aldrich), NON-ESSENTIAL AMINO ACID ( DIG-F12 (manufactured by SIGMA)) containing 200 mm mM L-GLUTAMINE (manufactured by SIGMA)) and non-adhesive dish (diameter 10 mm, diameter 10 mm) in the same medium for 10 days
- Actase solution manufactured by SIGMA
- NON-ESSENTIAL AMINO ACID DIG-F12 (manufactured by SIGMA)
- containing 200 mm mM L-GLUTAMINE manufactured by SIGMA
- non-adhesive dish disiameter
- the microarray was performed by Agilent®Expression® Array analysis (one-color method) (contract analysis by Takara Bio Inc.).
- the expression level in each cell was regarded as a relative numerical value, and clustered on a spherical self-organizing map to visualize the gene expression level in each cell.
- FIG. 18 it was found that there are a plurality of patterns in the phenotype of the mapping of the cell population including the cancer stem cells to be generated. This result suggests that, similarly to mice, cancer stem cells with different functions and properties are generated depending on the conditions used even if the same iPS cells are used.
- Example 3 Concentration of CD44-expressing cells using hyaluronic acid (1) Outline An outline of an experiment using human glioma-derived U251MG cells is shown in FIG. When subcultured cells cultured on a normal adhesive dish, transfer to a medium supplemented with hyaluronic acid (HA), culture in a non-adhesive dish, and further subculture to obtain a cell mass on the non-adhesive dish was made. After inoculating cell masses into nude mice to produce cancer-bearing cells, primary culture (referred to as U251MG-P1) was performed on an adhesive dish. Primary cultured cells were subcultured again on HA-added medium and cultured on non-adhesive dishes.
- HA hyaluronic acid
- FIG. 20 shows only the result of U251MG-P1
- a dense cell portion was formed, and partly Then, a cell mass was formed (FIG. 20.
- Each right end the state of the cells used was considered to be good (FIG. 20, each left end).
- the gene expression level was analyzed by qPCR method (FIGS. 24-1 to 24-3).
- U251MG cells and U251MG-P1 cells were also compared with RNA extraction from non-adhesive dishes and cells cultured on normal media.
- normal culture in a non-adhesive dish has increased CD44 gene expression, but the expression level is further increased by culturing on a non-adhesive dish and a medium supplemented with HA.
- the number of the cells was about 2.2 times that of the culture on the adhesive dish and the normal medium.
- the adhesion dish of U251MG cells and U251MG-P1 cells were cultured on a normal medium, and the CD44 gene expression level between these two cells was compared (FIG. 25).
- U251MG-P1 was about 3.9-fold higher in expression than U251MG. From these results, CD44 gene expression was induced by this culturing method, that is, spheroid culture of U251MG cells on a non-adhesive dish and HA-added medium, or cells with high CD44 gene expression level were selectively selected. It was found that it was possible to grow.
- the expression level of CD44 gene is constitutively higher than that of the parental cells. Was high (data not shown).
- CD44 protein expression level of cells spheroid-cultured in HA-added medium A172 cells and U251MG cells were respectively adhered to an adhesive dish and a normal medium (A), non-adhesive dish and a normal medium (N), and non-adherent It was cultured on a system dish and a medium supplemented with HA (H), and the expression level of CD44 protein was compared by Western blotting (FIG. 27). 10 ⁇ g of total protein extracted from the cells was migrated, and anti-CD44 mouse monoclonal antibody (Katayama Chemical Co., Ltd.) and HRP-labeled anti-mouse IgG antibody (CST Japan Co., Ltd.) were used as primary antibodies. Further, ⁇ -actin was detected as an internal indicator of constitutively expressed protein using an anti- ⁇ -actin antibody (CST Japan) and an HRP-labeled anti-rabbit IgG antibody (CST Japan).
- the expression level of CD44 protein was increased by culturing on a non-adhesive dish, and the expression level was further increased by culturing in a medium supplemented with HA.
- U251MG-P1 cells are glioma-derived cells
- U251MG cells, U251MG-P1 cells, and U251MG- P2 cells were cultured, and GFAP protein, which is an astrocyte marker protein, was detected by Western blotting (FIG. 28).
- a protein extracted from cells not expressing GFAP human ovarian cancer-derived SkOv-3 cells was used as a negative control.
- U251MG cell is represented as 0, U251MG-P1 cell as P1, U251MG-P2 cell as P2, and SkOv-3 cell as SkOv-3.
- U251MG-P1 and U251MG-P2 cells were confirmed to express GFAP when cultured in an adhesive dish.
- non-adhesive dish and HA-supplemented medium RPMI1640 containing 100 ⁇ g / ml sodium hyaluronate (sodium hyaluronate manufactured by Wako Pure Chemical Industries, Inc.), 10% FBS, 50 U / ml penicillin, and 50 ⁇ g / ml streptomycin)
- RPMI1640 containing 100 ⁇ g / ml sodium hyaluronate (sodium hyaluronate manufactured by Wako Pure Chemical Industries, Inc.), 10% FBS, 50 U / ml penicillin, and 50 ⁇ g / ml streptomycin
- RPMI1640 with 10% FBS, 50 U / ml penicillin, and 50 ⁇ g / ml streptomycin for 4 days (FIG. 29).
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Abstract
Description
1.がん細胞の培養上清の存在下でiPS細胞を培養すること含む、がん幹細胞を含む細胞集団を得る方法。
2.iPS細胞がヒト由来である、前記1に記載の方法。
3.2週間~2ヶ月間培養を行う、前記1または2に記載の方法。
4.得られた細胞集団ががん細胞を含む、前記1~3のいずれかに記載の方法。
5.前記1~4のいずれかに記載の方法により得られた細胞集団。
6.前記5に記載の細胞集団中のがん幹細胞を単離することを含む、がん幹細胞を得る方法。
7.前記6に記載の方法より得られたがん幹細胞。
8.がん治療薬のスクリーニング方法であって、以下の工程を含む方法:
(1)前記5に記載のがん幹細胞を含む細胞集団または前記7に記載のがん幹細胞と、がん治療薬の候補物質とを接触させる工程、および
(2)前記細胞集団または細胞に対する前記候補物質の作用を評価する工程。
9.前記細胞集団または細胞の増殖を阻害する候補物質を選択する工程をさらに含む、前記8に記載の方法。
10.前記5に記載のがん幹細胞を含む細胞集団と、がん治療薬の候補物質とを接触させる、前記8または9に記載の方法。
11.ヒアルロン酸の存在下でCD44発現細胞を含む細胞集団を非接着培養にて培養すること含む、CD44発現細胞の濃縮方法。
12.細胞集団がヒト由来である、前記11に記載の方法。
13.2~20日間培養を行う、前記11または12に記載の方法。
14.前記11~13のいずれかに記載の方法によりCD44発現細胞が濃縮された細胞集団。
iPS細胞は、脊椎動物、好ましくは哺乳動物由来の細胞である。哺乳動物としては、ヒト、マウス、ラット、ハムスター、ウサギ、イヌ、ネコ、ウシ、ウマ、ヒツジ、サル由来の細胞が挙げられる。iPS細胞は、好ましくは霊長類または齧歯類由来であり、さらに好ましくはヒトまたはマウス由来であり、さらにより好ましくはヒト由来である。iPS細胞としては、iPS-MEF-Ng-20D17、iPS-MEF-Ng-178B-5、iPS-MEF-Fb/Ng-440A-3、iPS-MEF-Ng-492B-4、iPS-Stm-FB/gfp-99-1、iPS-Stm-FB/gfp-99-3、iPS-Hep-FB/Ng/gfp-103C-1、iPS-L1、iPS-S1、253G1、409B2、454E2、HiPS-RIKEN-1A、HiPS-RIKEN-2A、HiPS-RIKEN-12A、Nips-B2などを用いることができる。
CD44は、1回膜貫通型糖蛋白質ヒアルロン酸レセプターであり、グリオーマ等のがん細胞の表面に特異的に高発現し、ヒトすい臓がん、前立腺がん、乳がんなどのがん幹細胞マーカーであることが知られている。CD44は、細胞凝集、細胞周囲のマトリックスの保持、マトリックス-細胞間のシグナリング、マトリックスメタロプロテアーゼ(MMP)の配向性制御(細胞遊走、浸潤)などの機能を有する。
(1)マウスiPS細胞の培養
マウスiPS細胞(iPS-MEF-Ng-20D17、理研セルバンクより購入)はDMEM培地 (15% FCS, 0.1mM NEAA, 2mM L-グルタミン, 0.1mM 2-メルカプトエタノール, 1000U/ml LIF, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)をもちい37℃、5%CO2下、MEF細胞上で培養維持した。本実験に用いたマウスiPS細胞は、未分化状態ではGFP遺伝子を安定に発現し、分化すると発現しなくなる(Okita, K. et al. Nature 448: 313-317 (2007)、参照により本明細書に含まれる)。
マウスルイス肺がん細胞株LLC細胞は10%血清を含むDMEM培地で培養した。マウス胚性癌腫細胞P19-CL6細胞は10%血清を含むαMEM培地(10%FCS, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)で培養した。マウス悪性黒色腫細胞B16-F10細胞、及び乳がん細胞BALB MC.E12細胞は10%血清を含むMEM培地(10%FCS, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)で37℃、5%CO2下で培養した。各々の細胞をコンフルエント状態まで培養し、その培養上精を回収、0.45μmのフィルターをもちいて濾過し、コンディションドメディウムとした。
7x105個のマウスiPS細胞を60mm径のゼラチンコートディッシュに播種しなおし、DMEM培地 (15% FCS, 0.1mM NEAA, 2mM L-グルタミン, 0.1mM 2-メルカプトエタノール, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)で培養した。細胞を播種した翌日より毎日、培地の半量を各種がん細胞より調整したコンディションドメディウムに置き換え4週間培養した。マウスiPS細胞に増殖が確認された場合はトリプシン処理により細胞を回収し、7x105個の細胞をDMEM培地 (15% FCS, 0.1mM NEAA, 2mM L-グルタミン, 0.1mM 2-メルカプトエタノール, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)を用いて、新しい60mm径のゼラチンコートディッシュに播種しなおし、8時間後に当量のコンディションドメディウムを加え培養を継続した。本培養を4週間継続した。誘導後の細胞はDMEM培地 (15% FCS, 0.1mM NEAA, 2mM L-グルタミン, 0.1mM 2-メルカプトエタノール, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)で維持した。
0.4μg/ml マイトマイシンCを処理して増殖阻害を誘導した各種がん細胞(1.5x105個)を60mm径ゼラチンコートディッシュに播種し、翌日マウスiPS細胞(5x105個)を播種した。マウスiPS細胞の増殖が見られた場合は継代を行い、4週間誘導を行った。コントロールとして、フィーダー細胞であるMEF細胞と共培養したiPS細胞を用いた。
iPS細胞より誘導したがん幹細胞の造腫瘍能の評価はヌードマウス(Balb/c Slc-nu/nu 6-8週齢 メス)を用いて行った(図1)。4x106個のmiPS-LLCcm細胞を100μlのDMEM培地(10%血清を含む)に懸濁し、ヌードマウスの背中に皮下注射により移植した。4週間後、形成された腫瘍を切除し、10%中性ホルマリン緩衝液(和光純薬)で24時間固定後、パラフィン包埋し、3μmの厚さの切片を作成し、ヘマトキシリン/エオシン染色を行った。他の細胞についても同様に実験を行った。
c:共培養
腫瘍切片はキシレンを用いて脱パラフィン処理し、10mMクエン酸緩衝液(pH6)中で95℃5分加熱し、その後プロテアーゼK消化(37℃、30分)により抗原の賦活化を行った。抗GFP抗体(岡山大学佐藤あやの准教授より提供、1:200)、抗サイトケラチン抗体(抗pan-Cytokeratin(AE1/AE3)抗体、Santa Cruz社 1:200)、抗CD31抗体(Santa Cruz社 1:200)を用いて染色を行った。ビオチン化二次抗体(Vector社)、ABC reagent (Vector社)、DAB(Vector社)により発色させ、対比染色としてヘマトキシリン染色をおこなった。
1.4x105個のmiPS-LLCcm細胞をMatrigel(登録商標)(BD社)コートした8 ウェル チャンバースライド(BD社)に播種し、EGM2培地(Takara社)を用いて24時間培養し、血管管腔様構造を形成させた。その後、4%パラホルムアルデヒドで細胞を固定し、抗CD31抗体(Santa Cruz社 1:200)、Texas Red標識二次抗体をもちいて免疫染色を行った。DAPIにより細胞の核を染色し、蛍光顕微鏡、共焦点顕微鏡で観察した。
miPS-LLCcm細胞がヌードマウスに形成した腫瘍より公知の方法で初代培養を行い、miPS-LLCcm primary細胞を樹立した。この細胞は、GFP陽性の細胞とGFP陰性の細胞(GFP陽性細胞より分化した細胞と考えられる)を含んでいる。この細胞を、トリプシン処理により、単細胞まで分離し、2 x 104個/mlで非表面処理ディッシュに播種した(浮遊培養)。培地は、血清を含まないDMEM培地 ( 0.1mM NEAA, 2mM L-グルタミン, 0.1mM 2-メルカプトエタノール, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)を使用した。培養は7~10日行い、スフェロイドの形成を観察した。形成されたスフェロイドはGFP陽性であった。この結果は、スフェロイドは足場非依存的に、単一の細胞より形成され、且つ、未分化状態であることを示しており、miPS-LLCcm細胞の自己複製能を示すものである(図5)。スフェロイドをトリプシンにより単一細胞まで分離したのち、1x105個以上の細胞数でヌードマウスに移植すると、再び悪性腫瘍を形成した。その形態は、初代の腫瘍と同様の、GFP陽性細胞、CK陽性細胞、および両者が陰性の細胞からなり、新生血管に富んだものであった。(図6)。この結果は、miPS-LLCcm細胞は移植を繰り返しても形態的に同様の腫瘍を形成していることを示している。
上記(8)のスフェロイドより回収した細胞1x105個をヌードマウスの尾静脈より移植し、4週間後、肺に形成された腫瘍を観察した。その腫瘍より公知の方法で初代培養を行いmiPS-LLCcm LMT細胞を樹立した。この細胞は、DMEM培地 (15% FCS, 0.1mM NEAA, 2mM L-グルタミン, 0.1mM 2-メルカプトエタノール, 50U/ml ペニシリン, 50U/ml ストレプトマイシン含有)で維持した。miPS細胞、miPS-LLCcmスフェロイド、miPS-LLCcm LMTスフェロイド、およびLLC細胞よりRNeasy kit (50) (QIAGEN)を使用しRNAを精製し、RT-PCRによりp53遺伝子およびMMP2遺伝子の発現量を比較した。
miPS細胞(フィーダー培養およびフィーダーレス培養)、miPS-LLCcm細胞、miPS-LLCcm primary細胞、およびmiPS-LLCcmスフェロイド、並びにそれらが形成するテラトーマおよび悪性腫瘍内での幹細胞マーカー遺伝子の発現を、RT-PCRによって確認した。RNeasy kit (50) (QIAGEN)(細胞からの精製)およびTRIzol(Invitrogen)(組織からの精製)を使用した。未分化マーカー遺伝子は Takahashi, Yamanakaの報告(Cell 126, 663-676, 2006、参照により本明細書に含まれる)に提示されているものを検証した。対照として、フィーダーに用いたMEF細胞の発現を確認した。その結果、本発明で得られた細胞群では、未分化マーカー遺伝子の発現が確認され、また、腫瘍においてもテラトーマと比較して発現が上昇しているものが多く見られた(図8)。
本実験で得た細胞集団について、RNeasy kit (50) (QIAGEN)を用いた方法、もしくはTRIzol (Invitorogen)を用いた方法でRNAを抽出した。その後、50μg以下のRNAに対してDNase I処理を行い、再度RNeasy kit (50) (QIAGEN) にて精製を行った。
(1)がん細胞の培養上清(コンディションドメディウム)の調製
表2に示すがん細胞株を、10% FBS,1% ペニシリン/ストレプトマイシンを含むDMEM培地あるいはRPMI1460培地で、接着培養用ディッシュ(直径100 mm, TPP社製)を用いて、37℃、5% CO2条件下で培養した。
ヒトiPS細胞(201B、RIKEN BRC)(Takahashi, K. et al., Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872 (2007)、参照により本明細書に含まれる)をLaminin-5(リプロセル社製)コートしたディッシュ(直径60 mm, TPP社製)に播種し、5 ng/mlのbFGFを含むRepro FF2培地(リプロセル社製)を用いて、37℃、2% CO2条件下で培養した。フィーダレスで未分化安定状態になるまで、毎日、培地を全量交換し、継代は一週間で一回の頻度でおこなった。
がん細胞株から回収したそれぞれのコンディションドメディウムと、Repro FF2またはRepro stem(リプロセル社製)、もしくはbFGFを添加していないヒトiPS幹細胞用培地(NON-ESSENTIAL AMINO ACID、200 mM L-GLUTAMINE、KSR、および0.1M 2-MERCAPTOETHANOL/PBSを含むDMEM-F12培地)とを1:1の比で混合し、分化誘導培地とした。未分化で安定な状態のヒトiPS細胞にそれぞれの誘導化培地を曝露し、分化を誘導した。分化誘導中は毎日、半量の培地交換を行い、2週間に1~2回の継代をおこなった。分化誘導期間は少なくとも28日間、長くとも2か月間とした。培養は37℃、2% CO2条件下でおこなった。
(1)概略
ヒトグリオーマ由来U251MG細胞を用いた実験の概略を図19に示した。通常の接着系ディッシュ上で培養した細胞を継代する際、ヒアルロン酸(HA)を添加した培地中に移して非接着系ディッシュで培養し、さらに継代して非接着系ディッシュ上で細胞塊を作製した。細胞塊をヌードマウスに接種して担がんを作製した後、接着系ディッシュ上で初代培養(U251MG-P1とした)を行った。初代培養細胞を再びHA添加培地に継代して非接着ディッシュ上で培養した。
ヒトグリオーマ由来A172細胞およびU251MG細胞、並びにU251MG-P1細胞をそれぞれ非接着ディッシュ上でHAを添加した培地(100 μg/ml ヒアルロン酸ナトリウム(和光純薬社製 ヒアルロン酸ナトリウム,鶏冠製)、10% FBS、50 U/ml ペニシリン、および 50 μg/ml ストレプトマイシンを含むDMEM)上と添加していない通常の培地(10% FBS、50 U/ml ペニシリン、および 50 μg/ml ストレプトマイシンを含むDMEM)上で2~5日間培養した。HAを添加しない場合には殆ど細胞が増殖しなかったが(図20にはU251MG-P1の結果のみを示した)、HAを添加した場合には細胞の密度が濃い部分が形成され、一部では細胞塊が形成された(図20.各右端)。またこれらの細胞は通常の接着系ディッシュ上では増殖が認められたので用いた細胞の状態は良好であると考えられた(図20.各左端)。
HA添加培地で作製したA172細胞、U251MG細胞、およびU251MG-P1細胞の細胞塊をAccutase溶液(Sigma-Aldrich社製)で懸濁して細胞を分散させた後、それぞれを再びHA添加培地、非接着系ディッシュ上で2週間培養した(図23)。いずれの細胞も再び細胞塊を形成し、細胞塊の周辺にはディッシュ表面に接着した細胞が観察され、細胞塊の自己複製能があることが示された。
A172細胞、U251MG細胞、およびU251MG-P1細胞を接着系ディッシュおよび通常培地上または非接着系ディッシュおよびHA添加培地上でそれぞれ培養した細胞からRNAを抽出し、CD44遺伝子発現量をqPCR法によって解析した(図24-1~図24-3)。U251MG細胞とU251MG-P1細胞は、非接着系ディッシュおよび通常培地上で培養した細胞からもRNA抽出と比較を行った。
HA添加培地で5日間培養した後、スフェロイド培養(100 μg/ml ヒアルロン酸ナトリウムおよびITS(Sigma-Aldrich社製)を添加した培地での培養)を2週間、非接着系ディッシュ(直径10 mm, 培地量10 ml)で行い、U251MG細胞をヌードマウスBalb/c-nu/nu、メス、4週齢に移植して4週間観察した(図26)。1~3枚のディッシュから集めた細胞をそれぞれマウスの左腹部に移植した。右腹部には通常培養したU251MG細胞を107個、それぞれ移植した。ディッシュ2枚以上から集めた細胞の移植によって担がんが形成された。107個の細胞を移植後40日目の腫瘍体積(腫瘍短辺2x腫瘍長辺)/2)は、1642.8 mm3 (n=2)であった。通常培養したU251MG細胞では同条件で同細胞数を移植しても腫瘍が形成されなかったことから、本発明の方法により得られた細胞集団は腫瘍形成能を有していることが示された。
A172細胞およびU251MG細胞それぞれを、接着系ディッシュおよび通常培地上(A)、非接着系ディッシュおよび通常培地上(N)、および非接着系ディッシュおよびHA添加培地上(H)で培養し、CD44タンパク質発現量をウエスタンブロッティング法によって比較した(図27)。細胞から抽出した総タンパク質を10 μgずつ泳動し、1次抗体として抗CD44マウスモノクローナル抗体(片山化学社製)およびHRP標識抗マウスIgG抗体(CSTジャパン社製)を用いた。また、抗β-アクチン抗体(CSTジャパン社製)とHRP標識抗ラビットIgG抗体(CSTジャパン社製)を用いて、構成的に発現しているタンパク質の内部指標として、β-アクチンを検出した。
U251MG-P1細胞がグリオーマ由来の細胞であることを確認するため、接着系ディッシュおよび通常培地上で、U251MG細胞、U251MG-P1細胞、およびU251MG-P2細胞(U251MG-P1細胞をヌードマウスに移植し、担がんを摘出して初代培養した細胞)を培養して、アストロサイトのマーカータンパク質であるGFAPタンパク質をウエスタンブロッティング法で検出した(図28)。また、GFAPを発現していない細胞(ヒト卵巣がん由来SkOv-3細胞)から抽出したタンパク質をネガティブコントロールとして用いた。それぞれの細胞から抽出した総タンパク質を10 μgずつ泳動して、抗GFAP抗体(Santa Cruz Biotechnology社製)とHRP標識抗マウスIgG抗体(CSTジャパン社製)を用いて検出した。構成的に発現しているタンパク質の内部指標として、β-アクチンを検出した。
SkOv-3細胞およびSkOv-3-P1細胞(SkOv-3細胞をマウスに接種後に担がんを摘出して初代培養した細胞)それぞれを、U251MG細胞と同様に、非接着ディッシュおよびHA添加培地(100 μg/ml ヒアルロン酸ナトリウム(和光純薬社製 ヒアルロン酸ナトリウム,鶏冠製)、10% FBS、50 U/ml ペニシリン、および 50 μg/ml ストレプトマイシンを含むRPMI1640)上、または非接着ディッシュおよび通常培地(10% FBS、50 U/ml ペニシリン、および 50 μg/ml ストレプトマイシンを含むRPMI1640)上で4日間培養した(図29)。
Claims (14)
- がん細胞の培養上清の存在下でiPS細胞を培養すること含む、がん幹細胞を含む細胞集団を得る方法。
- iPS細胞がヒト由来である、請求項1に記載の方法。
- 2週間~2ヶ月間培養を行う、請求項1または2に記載の方法。
- 得られた細胞集団ががん細胞を含む、請求項1~3のいずれかに記載の方法。
- 請求項1~4のいずれかに記載の方法により得られた細胞集団。
- 請求項5に記載の細胞集団中のがん幹細胞を単離することを含む、がん幹細胞を得る方法。
- 請求項6に記載の方法より得られたがん幹細胞。
- がん治療薬のスクリーニング方法であって、以下の工程を含む方法:
(1)請求項5に記載のがん幹細胞を含む細胞集団または請求項7に記載のがん幹細胞と、がん治療薬の候補物質とを接触させる工程、および
(2)前記細胞集団または細胞に対する前記候補物質の作用を評価する工程。 - 前記細胞集団または細胞の増殖を阻害する候補物質を選択する工程をさらに含む、請求項8に記載の方法。
- 請求項5に記載のがん幹細胞を含む細胞集団と、がん治療薬の候補物質とを接触させる、請求項8または9に記載の方法。
- ヒアルロン酸の存在下でCD44発現細胞を含む細胞集団を非接着培養にて培養すること含む、CD44発現細胞の濃縮方法。
- 細胞集団がヒト由来である、請求項11に記載の方法。
- 2~20日間培養を行う、請求項11または12に記載の方法。
- 請求項11~13のいずれかに記載の方法によりCD44発現細胞が濃縮された細胞集団。
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JP2016106617A (ja) * | 2014-12-05 | 2016-06-20 | 国立大学法人 岡山大学 | 被検物質のがん幹細胞誘導性評価技術 |
WO2016170938A1 (ja) * | 2015-04-20 | 2016-10-27 | 国立大学法人岡山大学 | がんの非ヒトモデル動物及びその作製方法、がん幹細胞及びその製造方法 |
JPWO2016170938A1 (ja) * | 2015-04-20 | 2017-04-27 | 国立大学法人 岡山大学 | がんの非ヒトモデル動物及びその作製方法、がん幹細胞及びその製造方法 |
JP2017086091A (ja) * | 2015-04-20 | 2017-05-25 | 国立大学法人 岡山大学 | がんの非ヒトモデル動物及びその作製方法、がん幹細胞及びその製造方法 |
JP2019505221A (ja) * | 2016-02-19 | 2019-02-28 | プロセラ セラピューティクス アーベー | ヒト心室前駆細胞の生着のための遺伝子マーカー |
US11725244B2 (en) | 2016-02-19 | 2023-08-15 | Procella Therapeutics Ab | Genetic markers for engraftment of human cardiac ventricular progenitor cells |
US11401508B2 (en) | 2016-11-29 | 2022-08-02 | Procella Therapeutics Ab | Methods for isolating human cardiac ventricular progenitor cells |
JP2022521529A (ja) * | 2019-02-22 | 2022-04-08 | 博瑞生物医薬(蘇州)股▲分▼有限公司 | Cd44標的化マルチアームコンジュゲート |
JP7353378B2 (ja) | 2019-02-22 | 2023-09-29 | 博瑞生物医薬(蘇州)股▲分▼有限公司 | Cd44標的化マルチアームコンジュゲート |
JP7514348B2 (ja) | 2019-06-10 | 2024-07-10 | プレクソジェン インコーポレイテッド | ヒアルロン酸を含む、幹細胞由来エクソソーム生成促進及び幹細胞能増強用組成物 |
WO2023000687A1 (zh) * | 2021-07-22 | 2023-01-26 | 浙江大学 | 一种人原发性骨髓纤维化细胞株及其应用 |
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US20150079626A1 (en) | 2015-03-19 |
EP2977450A1 (en) | 2016-01-27 |
JPWO2014148562A1 (ja) | 2017-02-16 |
EP2977450A4 (en) | 2016-12-14 |
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