WO2014123143A1 - Dispositif d'inspection de milieu de culture, système d'enregistrement d'informations de milieu de culture, système de maintien de l'hygiène et programme - Google Patents

Dispositif d'inspection de milieu de culture, système d'enregistrement d'informations de milieu de culture, système de maintien de l'hygiène et programme Download PDF

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Publication number
WO2014123143A1
WO2014123143A1 PCT/JP2014/052645 JP2014052645W WO2014123143A1 WO 2014123143 A1 WO2014123143 A1 WO 2014123143A1 JP 2014052645 W JP2014052645 W JP 2014052645W WO 2014123143 A1 WO2014123143 A1 WO 2014123143A1
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WIPO (PCT)
Prior art keywords
medium
information
culture
inspection
colony
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PCT/JP2014/052645
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English (en)
Japanese (ja)
Inventor
将慶 籠田
竜馬 備瀬
勇司 都築
京谷　均
累 齋藤
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大日本印刷株式会社
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Priority to JP2014530049A priority Critical patent/JP5605529B1/ja
Publication of WO2014123143A1 publication Critical patent/WO2014123143A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Definitions

  • the present invention relates to a food hygiene management system and the like.
  • the characteristics of an image obtained by imaging a colony vary depending on the type of colony (bacterial species) or the type of specimen.
  • an operator adjusts various parameters to image a colony. Some colonies are counted in a state suitable for the image characteristics.
  • Patent Document 1 a system that specifies a profile suitable for the image of the imaged medium and counts colonies by the specified profile.
  • the culture medium is different from the start of culture, or if the culture conditions are different as typified by the dilution rate and specimen extraction conditions, or if the specimen is different, It is difficult to perform tests on different media using.
  • the present invention has been made in order to solve the above-mentioned problems, and its purpose is to change conditions related to the culture environment of the colony when performing an inspection on the medium such as detection of colonies cultured in the medium. Even if it is a case, it is providing the culture medium information registration system etc. which can perform a test
  • the culture medium information registration system and the like performs a predetermined test on the culture medium using a culture medium image obtained by imaging the culture medium, and registers the result of the test as culture medium information.
  • a medium information registration system comprising: medium image data having a medium image of a medium used when the predetermined test is performed, and conditions regarding a culture environment of colonies cultured in the medium when the test is performed.
  • a first condition information that is stored in association with at least the condition information and an inspection criterion information that indicates an inspection criterion for detecting the colony based on the acquired condition information.
  • An inspection unit that performs the predetermined inspection on the medium imaged in an image; and a registration unit that registers information including at least the result of the predetermined inspection in the second database as medium information.
  • the medium information registration system and the like according to the present invention include, for example, conditions that vary depending on the timing at which a colony is inspected (culture time from the start of culture to the inspection time), and conditions that are determined based on the sample, such as the type of the sample placed on the medium. Or, when the culture environment of the colonies that are cultured in the medium when performing the test is different, such as the culture conditions that indicate the presence or absence of a filter when the diluent or specimen used for the test is extracted and placed on the medium.
  • the culture medium information registration system and the like according to the present invention can execute an accurate test even when the culture environment of the colonies to be cultured is different, and as a result, the test result as the culture medium information can be accurately determined. Can be registered.
  • the following embodiment is a medium information registration system, program, and the like according to the present invention for a hygiene management system using a film-type medium in a food production line or an inspection line (hereinafter referred to as “work line”). It is embodiment at the time of applying a colony detection apparatus and a hygiene management system.
  • the present invention is not limited to the following embodiments within the scope including the technical idea.
  • FIG. 1 is a block diagram which shows the structure of the sanitary management system S of this embodiment.
  • the sanitary management system S of the present embodiment uses a film-type culture medium (that is, a film-type culture medium) and performs data management on information on the culture medium (hereinafter referred to as “medium information”), thereby producing food. Or it is a system for inspecting the sanitary condition on inspection.
  • a film-type culture medium that is, a film-type culture medium
  • medium information information on the culture medium
  • the sanitary management system S of the present embodiment is configured to process the food being manufactured or manufactured in the work line 80 having a manufacturing process for manufacturing a processed or processed food or an inspection process for inspecting the food.
  • This is a system for data management of the culture situation when a preliminarily specified bacterium such as general bacteria or Escherichia coli, which has been extracted as a specimen and generated in the specimen in the film-type medium 60, is cultured.
  • the hygiene management system S of the present embodiment manages and determines the sanitary state of food on the data, information relating to work in food production or inspection (hereinafter referred to as “work information”), and the extracted sample. And the culture medium information of the film-type culture medium 60 acquired by the portable communication terminal device 10 before and after the culture is associated with the server device 40 (specifically, the database 400). is doing.
  • the sanitary management system S of the present embodiment is the film type medium 60
  • Conditions relating to the culture environment of colonies cultured in the film-type medium 60 when the medium-type image data including the image of the medium-type image obtained by imaging the film-type medium 60 is acquired and a predetermined test is executed ( That is, it acquires condition information (hereinafter referred to as “inspection condition information”) indicating inspection conditions when inspecting colonies generated in the film-type medium 60, and (2) Based on the acquired examination condition information, a colony detection standard (hereinafter referred to as “colony detection standard”) cultured in the film-type medium 60 is specified, and based on the specified colony detection standard. Perform a predetermined test by analyzing the media image, (3) A configuration for registering a predetermined inspection result in the database 400 is provided.
  • the sanitary management system S of the present embodiment includes a plurality of portable communication terminal devices 10 used when registering culture medium information regarding each film-type culture medium 60.
  • the administrator terminal device 20 that manages the sanitary management system S, the network 30, and the database 400, and in conjunction with the portable communication terminal device 10 or the administrator terminal device 20, registration management of medium information is performed.
  • a server device 40 that executes various types of processing.
  • the work information of this embodiment includes, in addition to various types of information related to the manufacturing process or inspection process, food extracted as a specimen from the manufacturing process or inspection process and information related to the culture of the specimen, and is unique to each work. It is registered in the database 400 using identification information (hereinafter referred to as “work ID”). Specifically, each work information includes (1) Work ID, (2) Identification information (hereinafter referred to as “line ID”) of the work line 80 from which the sample is to be extracted or, if the work line 80 has a plurality of processes, the line ID and process identification information (hereinafter referred to as “line ID”).
  • process ID 71b
  • Product name of food as a sample identification information of lot (hereinafter referred to as “lot ID”), lot name, (4) work date and time indicating the work start time and end time; (5) Worker ID (employee ID) and worker name, Is included.
  • Each piece of work information is registered in the database 400 for each type and for each work.
  • the portable communication terminal device 10 is, for example, a digital camera having a communication function, a tablet-type information terminal device, a smartphone or a portable telephone, or the like, or an imaging function such as a still image or a recording function (hereinafter simply referred to as “camera function”). And a communication terminal device portable by an operator.
  • the portable communication terminal device 10 can generate a culture medium image data by imaging the film-type culture medium 60 using a camera function, and can generate an instruction sheet image data by imaging the work instruction sheet 70. It has a configuration. And the portable communication terminal device 10 can register work ID, the culture-medium image data of the film-type culture medium 60, and the metadata of the said culture-medium image data in the database 400 of the server apparatus 40 as culture-medium information. It has a configuration.
  • the portable communication terminal device 10 transmits BLUETOOTH (registered trademark), wireless LAN (WLAN: Wireless Local Area Network), or wireless PAN (WPAN: Wireless Personal Area Network). Is transmitted to the server apparatus 40 directly or via the access point 50 using a communication standard for short-range wireless communication, or the like, or is transmitted to the server apparatus 40 using a public telephone network line via a mobile base station (not shown). It has a configuration. And the portable communication terminal device 10 has a browser function constructed by a markup language such as XML (extensible Markup Language), and executes an operation input instruction and an operation confirmation of an operator using the browser function. The culture medium information is transmitted to the server device 40 via the browser function.
  • BLUETOOTH registered trademark
  • WLAN Wireless Local Area Network
  • WPAN Wireless Personal Area Network
  • the administrator terminal device 20 also manages administrator identification information (hereinafter referred to as “manager ID”), administrator password, and worker identification information (hereinafter referred to as “worker ID”). It functions as a control device capable of performing management of access authority to the server device 40, management of the terminal ID of the portable communication terminal device 10, correction of registered medium information, and other management.
  • manager ID administrator identification information
  • worker ID worker identification information
  • the administrator terminal device 20 has a browser function built in a markup language, like the portable communication terminal device 10, and uses the browser function to exchange data with the server device 40 and to generate a report. It has a configuration capable of executing browsing and the like.
  • the administrator terminal device 20 is configured so that, in the same lot, when each operation is interrupted due to a failure of a machine that executes a process or the like, when a registration error of medium information or the like occurs in the operator, medium information or other information It has the structure which can correct.
  • the network 30 includes, for example, a public telephone line network (hereinafter referred to as “long-distance communication network”) including a mobile phone network, an IP (Internet Protocol) network such as a short-distance wireless network, or both of which are interconnected.
  • long-distance communication network including a mobile phone network, an IP (Internet Protocol) network such as a short-distance wireless network, or both of which are interconnected.
  • IP Internet Protocol
  • the configuration of the network 30 is not limited to this.
  • the server device 40 is used in conjunction with the portable communication terminal device 10 or the administrator terminal device 20, and is used to execute each data processing for performing hygiene management of lots and work lines 80 and registration of medium information. It is.
  • the server device 40 after a lapse of a predetermined time from the start of culture, (1) Medium image data having a medium image obtained by imaging the film-type medium 60 used when the inspection is performed, and colonies cultured in the film-type medium 60 when the predetermined inspection is performed Condition information (inspection condition information) indicating conditions (inspection conditions) regarding the culture environment of (2) Search the database 400 based on the acquired inspection condition information, specify the colony detection criteria for colonies cultured in the film-type medium 60, (3) detecting a feature amount relating to the color of each pixel constituting the culture medium image; (4) Based on the culture medium image of the acquired culture medium image data, the detected feature amount, and the specified colony detection criterion, a predetermined inspection is performed on the film-type culture medium 60 imaged in the culture medium image, (5) It has a configuration in which information including at least a result of a predetermined test is registered in the database 400 as culture medium information.
  • the examination condition information includes at least one of conditions determined based on the sample placed on the film-type medium 60 (hereinafter referred to as “sample conditions”) and culture conditions determined when the colonies are cultured. Conditions are included.
  • the sample condition includes the extraction method for extracting the sample such as the type of the sample, the type of the process for extracting the sample, and the presence or absence of the filter
  • the culture condition includes the type of the diluent, the dilution factor Incubation temperature and cultivation humidity are included.
  • the specific information includes at least one of unique identification information given to each film type medium 60 (that is, medium ID 66) and the type of film type medium 60.
  • conditions that vary depending on the timing of inspecting the colony (culture time from the start of culture to the test time), and the type of specimen placed on the medium
  • conditions determined based on the specimen or the culture conditions that indicate the presence or absence of a filter when the diluent or specimen used for the test is extracted and placed on the culture medium.
  • the medium can be inspected, such as determination of
  • an accurate inspection can be performed even when the culture environment of the colonies to be cultured is different, and as a result, the inspection result as the medium information is accurately detected. Can be registered.
  • hygiene management related to food using the film-type culture medium 60 can be analyzed by electronic data. At the same time, the analysis result can be provided electronically.
  • the sanitary management system S of the present embodiment it is possible to save resources compared to the case where various data analysis and provision are performed using physical resources such as paper media, and the culture medium.
  • registration errors can be significantly reduced and difficult media management can be facilitated, so resources can be saved without wastefully consuming media. Yes.
  • FIG. 2 is an example of a film-type medium 60 used in this embodiment.
  • the film-type medium 60 used in the present embodiment is a medium for culturing bacteria generated in food as a specimen detected from each step of the work line 80 using a film or sheet-like dry medium.
  • the film-type culture medium 60 is used as a culture medium for cultivating general live bacteria, coliform bacteria, and Staphylococcus aureus.
  • the film-type medium 60 includes, for example, a base sheet 61 formed of a film and a circular frame formed on the base sheet 61 with reference to the center of the base sheet 61 as shown in FIG. (Hereinafter referred to as “circular frame”) 62, a culture layer 63 for culturing bacteria provided in the frame, a cover sheet 64 covering the culture layer 63, and the left side of the base sheet 61.
  • Medium ID66 a base sheet 61 formed of a film and a circular frame formed on the base sheet 61 with reference to the center of the base sheet 61 as shown in FIG.
  • circular frame 62
  • a culture layer 63 for culturing bacteria provided in the frame
  • cover sheet 64 covering the culture layer 63
  • left side of the base sheet 61 The film-type medium 60 includes, for example, a base sheet 61 formed of a film and a circular frame formed on the base sheet 61 with reference to the center of the base sheet 61 as shown in FIG. (Hereinafter referred to as “
  • the culture medium ID 66 is formed of a barcode such as a two-dimensional barcode or alphanumeric characters, and is imaged together when the film-type culture medium 60 is imaged, such as a barcode recognition function or an OCR (Optical Character Recognition) function. It is acquired in the portable communication terminal device 10 or the server device 40 using the image analysis function.
  • a barcode such as a two-dimensional barcode or alphanumeric characters
  • OCR Optical Character Recognition
  • the substrate sheet 61 is not particularly limited as long as it is a film-like or sheet-like substrate, and for example, a plastic film, paper, or the like can be used.
  • plastic films include resin films such as polyethylene, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polymethacrylate, polymethyl methacrylate, polymethyl acrylate, polyester, and polycarbonate.
  • Preferred examples include polyethylene terephthalate and polypropylene synthetic paper.
  • the polypropylene-based synthetic paper is a film synthetic paper whose main raw material is polypropylene.
  • the film-type culture medium 60 has an IC tag in which a culture medium ID 66 is stored.
  • the short-range wireless communication interface 140 (for example, an IC tag reader) included in the portable communication terminal apparatus 10 causes the medium to be connected to the portable communication terminal apparatus 10. ID66 may be acquired.
  • FIG. 3 is an example of a work instruction sheet 70 used in the present embodiment.
  • the work instruction sheet 70 of the present embodiment includes, for each food group manufactured or inspected under the same conditions, that is, for each lot, work names and contents of one or more manufacturing processes or inspection processes, and work lines 80 to be used. It is an instruction document that describes the name of the food (processed food) to be manufactured or inspected, the quantity, and other information.
  • the work instruction sheet 70 of the present embodiment has an instruction sheet ID 71a formed by printing or other methods on the upper right and a plurality of process IDs 71b formed by printing or other methods on a predetermined area. .
  • the instruction sheet ID 71a and each process ID 71b are used as the work ID 71, and are formed of, for example, a barcode such as a two-dimensional barcode or alphanumeric characters.
  • the instruction ID 71a and each process ID 71b are imaged in the same manner as the film-type medium 60 when the registration of the film-type medium 60 is executed, and analyzed by the portable communication terminal device 10 or the server device 40. Be recognized.
  • the work instruction sheet 70 has an IC tag in which the work ID 71 of the instruction sheet ID 71a or the process ID 71b is stored, like the film type culture medium 60, and has an interface (short distance) that the portable communication terminal device 10 such as a tag reader has.
  • the portable communication terminal device 10 may acquire the work ID 71 by the wireless communication interface 140).
  • the work ID 71 indicates an instruction ID 71a or a process ID 71b.
  • FIG. 4 is a block diagram showing the configuration of the portable communication terminal device 10 of the present embodiment.
  • the portable communication terminal device 10 of the present embodiment has a data storage unit 100 having a memory function used when various programs are executed, an imaging function, image data of the film-type medium 60, and other image data.
  • the image data generation unit 110 that generates the media information the media information is registered in the server device 40 after the initial registration and the post-cultivation registration immediately after the culture in conjunction with the server device 40 (hereinafter referred to as “medium information registration processing”).
  • an application control unit 120 that executes processing.
  • the portable communication terminal device 10 includes a network communication unit 130 that communicates with the server device 40 and other communication devices, a short-range wireless communication interface 140 that exchanges data with an IC tag and other communication interfaces, Controls the current position detection unit 150 that detects the current position, the display unit 160, the display control unit 161 that controls the display unit 160, the operation unit 170 for inputting user operations, the timer 180, and the entire apparatus.
  • a portable terminal management control unit 190 A portable terminal management control unit 190.
  • the portable communication terminal device 10 has various necessary members such as a microphone, a speaker, and an e-mail transmission / reception function when having a mail function such as a telephone function and e-mail. Further, the above-described units are connected to each other by a bus 11 to exchange data.
  • the data storage unit 100 includes an application storage unit 101 that stores various application programs, an image data storage unit 102 that stores image data captured and generated by the image data generation unit 110, and a portable communication terminal device 10 and management / control programs, and a ROM / RAM 103 that is used as a work area during execution of each program and stores data used in each process executed by the portable communication terminal device 10. is doing.
  • the application storage unit 101 includes an application program (hereinafter referred to as “application”) that is executed by the application control unit 120 in conjunction with the image data generation unit 110, the operation unit 170, the display control unit 161, and the image data storage unit 102. Is recorded).
  • the application storage unit 101 also stores a browser program for realizing the above browsing function.
  • the imaged medium image data and instruction sheet image data In the image data storage unit 102, the imaged medium image data and instruction sheet image data, the image ID for managing each image data, various metadata corresponding to each image data such as the imaging time, and the work ID 71
  • the medium ID 66 and various flag information are stored in association with each other.
  • the image ID is arbitrary identification information that is appropriately given in each portable communication terminal device 10.
  • the image data generation unit 110 includes an optical system, a CCDI sensor (Charge Coupled Device Image Sensor) that converts an optical image input from the optical system into an electrical signal, and image data based on the electrical signal generated by the CCDI sensor. And a generating unit that generates
  • the application control unit 120 realizes medium information registration processing by a predetermined application.
  • the application control unit 120 executes the network communication unit 130 and the display control unit 161 while executing various control programs for controlling each unit of the portable communication terminal device 10 using the culture medium registration application stored in the application storage unit 101.
  • various processes are executed in conjunction with or controlled by the operation unit 170.
  • the application control unit 120 executes the medium registration application recorded in the application storage unit 101, controls the image data generation unit 110 to acquire the medium image data of the film-type medium 60 captured and the image data storage unit 102. To remember.
  • the application control unit 120 acquires the medium ID 66 from the imaged medium image data, and together with the acquired medium ID 66, the image data storage unit displays the medium image data, the metadata of the medium image data, and various flag information. 102.
  • the application control unit 120 may execute barcode recognition or OCR recognition in conjunction with another communication device or the database 400 via a communication line.
  • the application control unit 120 performs initial registration for registering the culture medium information in the server device 40 at the start of the culture of the specimen after imaging of the culture medium image data or at a predetermined timing, and registers the culture medium information after the start of the culture of the specimen. Perform registration after incubation.
  • the application control unit 120 identifies whether the registration is initial registration or post-cultivation registration based on the operator's instruction, and includes information (flag information) indicating either registration in the culture medium information in the server device 40. Send.
  • the application control unit 120 acquires the current time from the timer 180 when the film-type medium 60 is imaged, and the image data storage unit as the metadata of the medium image data together with a predetermined image ID using the current time as the imaging time. 102.
  • the medium image data is transmitted to the server device 40, it is transmitted together.
  • the application control unit 120 when the culture medium ID 66 and the work ID 71 that is the instruction ID 71a and the process ID 71b are stored in the IC tag, the operation unit 170, the display control unit 161, and the short-range wireless communication interface The medium ID 66 and the work ID 71 are acquired in conjunction with 140.
  • the application control unit 120 transmits instruction sheet image data in addition to the medium image data as the medium information.
  • the application control unit 120 may be realized as a function when the CPU (central processing unit) constituting the mobile terminal management control unit 190 executes the application in terms of implementation.
  • the network communication unit 130 establishes a communication line with the server device 40 connected to the network 30 under the control of the application control unit 120 and the mobile terminal management control unit 190, and exchanges various data such as culture medium image data. Do.
  • the near field communication interface 140 performs near field communication using an IC tag under the control of the application control unit 120 and the mobile terminal management control unit 190.
  • the current position detection unit 150 is a satellite transmitted from the GPS satellite while recognizing the position of GPS (Global Positioning System) hygiene via the network 30 under the control of the application control unit 120 or the mobile terminal management control unit 190.
  • a signal GPS signal is detected.
  • the current position detection unit 150 calculates (that is, detects) a coordinate value indicated by the latitude and longitude of the current position of the portable communication terminal device 10 based on the detected GPS signal. In addition, the current position detection unit 150 provides the calculated coordinate value to the application control unit 120 as position information.
  • the portable communication terminal device 10 when the portable communication terminal device 10 has a telephone function or a short-range wireless function, the portable communication terminal device 10 is based on the direction and the radio field intensity received by the telephone base station or the like for the radio wave used for the telephone or the short-range radio.
  • the current position of the portable communication terminal device 10 may be calculated (detected).
  • the display unit 160 has an image display area of a predetermined size (for example, 5 inches, W480 ⁇ H960 pixels), is configured by a panel of liquid crystal elements or EL (Electro Luminescence) elements, and is generated by the display control unit 161 A predetermined image is displayed based on the display data.
  • a predetermined size for example, 5 inches, W480 ⁇ H960 pixels
  • EL Electro Luminescence
  • a predetermined image is displayed based on the display data.
  • the display unit 160 is linked to the operation unit 170, and various displays, captured work instructions 70 and film-type culture media that have been imaged. 60 is displayed.
  • the display control unit 161 generates drawing data necessary to cause the display unit 160 to draw a predetermined image under the control of the application control unit 120 or the mobile terminal management control unit 190, and the generated drawing data is displayed on the display unit. 160 is output.
  • the operation unit 170 includes various confirmation buttons, operation buttons for inputting operation commands, a number of keys such as a numeric keypad, and a touch sensor provided on the display unit 160, and is used when performing each operation. It has become. Specifically, the operation unit 170 is used when performing operations for executing the various processes described above when the culture medium registration application is activated. In the present embodiment, the operation unit 170 can also be used when manually inputting the culture medium ID 66 directly.
  • the timer 180 provides the application control unit 120 with the date and time when the image data generation unit 110 images the film-type medium.
  • the portable terminal management control unit 190 is mainly configured by a central processing unit (CPU) and includes various input / output ports such as a key input port and a display control port, and the overall functions of the portable communication terminal device 10. In addition, overall functions for executing the information providing program are generally controlled.
  • CPU central processing unit
  • FIG. 5 is a configuration diagram showing the configuration of the server device 40 of the present embodiment.
  • the server device 40 of the present embodiment communicates with a database 400 in which various types of information such as work information and medium information are stored, and the portable communication terminal device 10 and the administrator terminal device 20.
  • the above-described units are connected to each other by a bus 41, and data transfer is performed between the components.
  • the communication control unit 410 is a predetermined network interface, establishes a communication line with the portable communication terminal device 10 or the administrator terminal device 20, and exchanges various data with the portable communication terminal device 10 or the administrator terminal device 20. I do.
  • the database 400 includes an HDD, and includes a medium information database (hereinafter abbreviated as “medium information DB”) 401, a line information database (hereinafter abbreviated as “line information DB”) 402, and a lot information database (hereinafter referred to as “line information DB”).
  • Lot information DB ”) 403 work management database (hereinafter abbreviated as“ work management DB ”) 404, and profile DB 405 storing profile information defining colony inspection standards used when performing a predetermined inspection.
  • the medium information DB 401 of the present embodiment constitutes the second database of the present invention
  • the profile DB 405 constitutes the first database of the present invention
  • the work management DB 404 of the present embodiment constitutes a third database of the present invention.
  • the culture medium information DB 401 is extracted for each process of each work line 80, and the culture medium information on the film-type culture medium 60 of the specimen obtained by being imaged and acquired by the portable communication terminal device 10 is set for each work ID. It is a database that is stored while being granted.
  • the culture medium information DB 401 (1) Medium ID (2) Work ID (3) Imaged medium image data (4) Image capture time (5) Other information including flag information (6) Number of colonies (7) Medium determination result (8) Size (number of pixels), barycentric position, each pixel Information on each detected colony such as feature amount (hereinafter referred to as “colony information”) (9) Type of profile information (profile ID) used at the time of colony detection Are recorded in association with each other.
  • the result determined by the data processing unit 420 is stored.
  • the colony information, the number of colonies, and the medium determination in each medium information may be registered when the medium information is registered, or may be registered at a predetermined timing different from the registration timing of the medium information.
  • the work ID may be a lot ID, and the sample to be extracted is the same lot.
  • the line ID or the process ID 71b is obtained.
  • the line information DB 402 is a database in which line information regarding each work line 80 is stored for each line ID.
  • the line information DB 402 includes (1) Line ID (2) The process ID 71b of the process included in the work line 80 and the two types of data are recorded in association with each other.
  • the name which identifies each process such as material injection
  • the lot information DB 403 is a database in which lot information regarding each lot is stored for each lot ID. For example, in the lot information DB 403, (1) Lot ID (2) Line ID of work line used for production or inspection of lots (3) Lot work start time (4) Lot work end time (5) Five pieces of data of a work team of workers who perform lot work are recorded in association with each other.
  • the work management DB 404 is a database that stores various types of information related to work contents.
  • the work management DB 404 includes (1) Work ID (2) Line ID (or line ID and process ID 71b) of the work line from which the sample is to be extracted (3) Type of sample (food) (product name, lot ID and / or lot name) (4) Examination type (generic bacteria or bacterial species to be detected such as Escherichia coli) and color (coloring) information (RGB gradation value or range) when it is generated on the film-type medium 60 (5) Culture conditions (dilution rate, type of diluent, culture temperature and culture humidity) (6) Culture start time (7) Culture time from the start of culture until a predetermined time has elapsed (8) Culture location (for example, the position of the incubator number and the number of steps in the incubator) (9) Information (hereinafter simply referred to as “presence / absence of use of filter”) indicating whether or not a filter is used when a specimen is extracted in order to remove extra solid matter such
  • a single time that is, an end time for ending the culture of the bacteria in the sample
  • a plurality of times such as 24 hours from the start of culture, 48 hours, or 72 hours may be set.
  • the profile DB 405 is a database that stores profile information (hereinafter also referred to as “inspection standard information”) indicating colony detection criteria used when colonies are detected. Specifically, in the profile DB 405, for each profile ID, for each sample type, bacterial species, culture time, and culture condition, (1) RGB, luminance, saturation, etc. A detection method for detecting a colony based on the types (2) and (1) of the feature amount related to the color in each pixel of the medium image used for detecting the colony imaged from the medium image, and the detection Parameters to execute, and (3) Threshold value for pass / fail of medium determination, Is recorded.
  • the data processing unit 420 executes various data processing in accordance with the application recorded in the ROM / RAM 440.
  • the data processing unit 420 executes a predetermined program, (1) Operation management of the communication control unit 410; (2) Medium information registration processing executed in conjunction with each portable communication terminal device 10; (3) Inspection condition information acquisition processing for searching the operation information DB 404 based on the medium ID 66 and operation ID acquired during the medium information registration processing and acquiring inspection condition information; (4) A profile specifying process for searching the profile DB 405 based on the acquired inspection condition information and specifying a colony detection reference (ie, profile) of a colony imaged in the medium image; (5) While detecting colonies imaged in the culture medium image based on the specified profile, colony detection determination processing for executing the count of the number of detected colonies and the medium determination based on the number of colonies; (6) lot determination processing for determining a lot based on registered medium information; (7) Report processing for outputting the determination result in a report format; (8) Management and control of the database 400;
  • the registration processing unit 421 of the present embodiment constitutes acquisition means and registration means according to the present invention
  • the colony detection determination unit 423 constitutes search means, detection means, and inspection means. Furthermore, details of the registration processing unit 421, the colony detection determination unit 423, the lot determination processing unit 424, and the report processing unit 425 of this embodiment will be described later.
  • the server management control unit 430 is mainly configured by a central processing unit (CPU), and integrally controls each unit of the server device 40 by executing a program. Specifically, the server management control unit 430 performs login processing for each user and various other controls based on a login request from the portable communication terminal device 10 by a user operation.
  • CPU central processing unit
  • ROM / RAM 440 Various programs necessary for driving the server device 40 are recorded in the ROM / RAM 440.
  • the ROM / RAM 440 performs clustering learning data used in the colony specifying process, medium determination (pass / fail determination) of each film-type medium 60, process determination, line determination, and lot determination.
  • medium determination criterion information Each information such as information on the determination criterion to be used (hereinafter also referred to as “medium determination criterion information”) is stored.
  • the ROM / RAM 440 is used as a work area during execution of each program.
  • the timer 450 is used for managing the time required when the medium information registration process and the lot determination process are executed.
  • the registration processing unit 421 executes a registration process for registering each medium information in the medium information DB 401 while being associated with the work ID 71 in conjunction with the portable communication terminal device 10.
  • the registration processing unit 421 executes different medium information registration processing based on flag information indicating whether the registration is initial registration or post-culture registration included in the culture medium information.
  • the registration processing unit 421 executes a registration process based on the initial registration.
  • the registration processing unit 421 when the registration processing unit 421 acquires the culture medium information via the communication control unit 410, the registration processing unit 421 specifies the work ID 71. In addition, the registration processing unit 421 searches the work management DB 404 based on the specified work ID, specifies the corresponding work information, and associates the acquired medium image data and imaging time with the specified work information. Is registered in the culture medium information DB 401.
  • the registration processing unit 421 specifies the lot ID including the work line 80 from which the sample is extracted based on the acquired work ID 71, the line ID of the work line 80, the process ID 71b from which the sample is extracted, and the specification.
  • the culture medium image data and the imaging time included in the culture medium information are registered in the culture medium information DB 401 while giving a unique culture medium ID in association with the lot ID, the line ID, and the process ID 71b.
  • the registration processing unit 421 registers the imaging time in the culture medium information DB 401 as the culture start time. Further, as the culture start time, a preset time may be used instead of the imaging time on the assumption that the medium information registration process is executed at the time.
  • the registration processing unit 421 performs a registration process based on post-cultivation registration.
  • the registration processing unit 421 when acquiring the culture medium information via the communication control unit 410, specifies the work ID 71 included in the culture medium information, as in the initial registration. Then, the registration processing unit 421 searches the work management DB 404 based on the specified work ID 71, specifies the corresponding work information, and associates the medium image data and the imaging time with the specified work information. Register in the information DB 401.
  • the culture examination time may be a preset time instead of the imaging time on the assumption that the culture information registration process is executed at the same time as the culture start time.
  • the registration processing unit 421 causes the colony detection determination unit 423 to execute the colony detection determination process based on the acquired medium information, and specifies the colony information, the number of colonies, and the determination result acquired as the medium information as the medium information.
  • the medium ID DB 401 is registered in association with the work ID 71.
  • the colony detection determination unit 423 executes an inspection condition information acquisition process, a profile identification process, and a colony detection determination process.
  • the colony detection determination unit 423 of the present embodiment configures the medium image in order to detect pixels that are supposed to form an imaged colony from (A) the medium image. Detecting a pixel having a characteristic amount relating to a color having a predetermined condition (hereinafter referred to as a “colony pixel”), (B) Link colony pixels based on a predetermined condition, execute labeling to give an ID, detect a connected colony pixel group as one colony candidate, (C) The colony candidate which has a predetermined condition is specified as a colony, the number is counted, (D) Medium determination is performed based on the counted number of colonies.
  • a characteristic amount relating to a color having a predetermined condition hereinafter referred to as a “colony pixel”
  • B Link colony pixels based on a predetermined condition, execute labeling to give an ID, detect a connected colony pixel group as one colony candidate
  • C The colony candidate which has a predetermined condition
  • the time from the start of culture in each film-type medium 60 to the time (hereinafter referred to as “inspection time”) at which the inspection (that is, the detection and counting of colonies) is performed.
  • the culture time is short (for example, 12 hours or 24 hours after the start of the culture)
  • the color generated on the film-type medium 60 and the change thereof Precise detection is prioritized over the presence or absence of colonies or their occurrence over the number of colonies.
  • the inspection standard such as the colony identification standard to be detected, such as the color of the colony, the detection method thereof, and the medium determination standard is changed according to the culture time from the start of culture to the test time.
  • the detection criteria for the colonies to be cultured also change, and in the process where the specimen is extracted, the baking process, Inspection criteria such as colony identification criteria, detection method and media judgment criteria according to the type or culture state of the bacteria to be detected by the step of cooking using a fire such as a cooking step or the step of cooking while maintaining the raw state Is different.
  • the culture conditions such as different dilutions and dilution ratios are different. If they are different, or whether the specimen is filtered when placed on the film-type medium 60, the color, size, and shape of the colonies are different, and the film type of non-objects other than the colonies Since the contamination factors on the culture medium 60 are different, even in such a case, the inspection standards such as the identification standard of the colonies to be detected, the detection method thereof, and the standard of the medium determination are different.
  • the colony detection determination unit 423 of the present embodiment acquires one or more pieces of inspection condition information predetermined by an administrator or predetermined by an application.
  • the work information as inspection condition information includes (1) Information that fluctuates at the timing when the film-type medium 60 is imaged and inspected, such as the culture time, (2) Information determined based on the sample placed on the film-type medium 60, such as the type of the sample, the type of the process of extracting the sample, or the presence or absence of a filter when extracting the sample (ie, filter information) (3) Culture conditions Information that is determined when culturing colonies such as is included.
  • the colony detection determination unit 423 acquires the specimen type, the bacterial species, the culture time, the culture condition, and the filter information indicating whether or not the filter is used as the test condition information.
  • the colony detection determination unit 423 includes the medium information received by the registration processing unit 421 from the portable communication terminal device 10 or the medium information registered in the medium information DB 401 and the work information DB 404 associated with the medium information. Inspection condition information is acquired from the work information stored in the database and other information stored in the database.
  • the colony detection determination unit 423 searches the work information DB 404 based on the imaging time and work information ID included in the received or registered medium information, and performs the corresponding work.
  • the culture time included in the work information having the ID is specified and acquired.
  • the colony detection determination unit 423 when the corresponding work information includes two or more culture times, calculates the time calculated based on the culture start time and the imaging time included in the acquired medium information (actual culture time). ) And a specific culture time within a certain range (for example, ⁇ 10 minutes), or a culture time closest to the actual culture time is specified and acquired.
  • the colony detection determination unit 423 searches the work information DB 404 based on the work information ID and associates it with the work ID corresponding to the work information DB 404.
  • the specimen type, filter information, or culture conditions recorded in this way are specified and acquired.
  • the colony detection determination unit 423 searches the line information DB 402 based on the work ID, and the line information DB 402 The process type recorded in association with the work ID corresponding to is specified and acquired.
  • the colony detection determination unit 423 searches the profile DB 405 based on one or more inspection condition information acquired by the inspection condition information acquisition process, and matches the inspection condition information 1 Alternatively, a profile having an inspection standard for two or more colonies is acquired.
  • the colony detection determination unit 423 acquires, as the test condition information, the type of the specimen, the bacterial species, the culture time, the culture condition, and the filter information indicating whether or not the filter is used, Search the profile DB 405 according to the inspection condition information, (1) Type of feature quantity related to color when detecting colony pixels from a medium image (2) Detection method for detecting a colony based on the type of (1), a parameter for executing the detection, and (3) Threshold value for pass / fail when performing medium determination, Is identified as a set.
  • the type of feature amount related to the color when detecting colony pixels from the medium image defined by the profile (1A) the feature amount of each RGB sub-pixel of each pixel; (1B) brightness, and (1C) Saturation, including.
  • the detection method for detecting colonies and the parameters for executing the detection include (2A) Colony pixel detection method, (2B) Colony candidate detection method, (2C) a method related to identification of a colony, and (2E) Various parameters used for each of the above detection methods, including.
  • the colony pixel detection method includes binarization based on a single feature amount to detect colony pixels, detection of colony pixels based on a plurality of feature amounts, The presence / absence of an effective region to be detected on the image or its region (for example, within a predetermined radius region from the center for invalidating the detection of the peripheral portion of the culture medium image) is included.
  • These parameters include a threshold value for binarization, a maximum value or a minimum value when a plurality of feature amounts are used, and a valid area range value.
  • colony candidate detection methods include the presence or absence of execution of image filter processing for reducing noise such as expansion processing or contraction processing, and a method for connecting adjacent colony pixels to make colony candidates (proximity reference). ) Is included. These parameters include various values for determining whether adjacent colony pixels constitute the same colony.
  • the method related to the identification of the colony includes a calculation method for calculating a likelihood indicating the colony-likeness of each colony candidate such as the size, shape and color characteristics of the colony.
  • These parameters include a threshold for determining whether or not the colony is based on the coefficient and likelihood used in the calculation method.
  • the parameters used for the medium determination include a threshold value used when the pass / fail determination is performed.
  • the corresponding specimen type is configured as a set of the above-described threshold values and parameters for the bacterial species and the culture time. Identify the profile
  • the colony detection determination unit 423 of the present embodiment determines a colony pixel detection method, a colony candidate detection method, a colony specification method, and a medium determination method while setting various threshold values or parameters based on the specified profile. While executing each process.
  • the colony detection determination unit 423 (1) When the type of feature quantity related to color when detecting a colony pixel defined in the profile is the feature quantity of each RGB sub-pixel of each pixel, the gradation value of each sub-pixel of each pixel And the parameters specified in the profile, (2) When the type of the feature quantity is luminance or saturation, the value obtained by executing a predetermined calculation based on the gradation value of each RGB sub-pixel of each pixel and the profile are specified. Based on the parameters Colony pixels are detected according to the colony pixel detection method defined in the profile.
  • the colony detection determination unit 423 binarizes the feature amount defined in the profile with the threshold defined in the profile. Then, a pixel having one value is detected as a colony pixel.
  • the colony detection determination unit 423 determines a maximum value and a minimum value that are predetermined in each feature amount defined by the profile. Are detected, and pixels belonging to the range from the maximum value to the minimum value for all feature quantities are detected as colony pixels.
  • the colony detection determination unit 423 detects only pixels belonging to the detection range as colony pixels. Further, the colony detection determination unit 423 temporarily stores each extracted colony pixel in the ROM / RAM 440.
  • the threshold when the culture time is short, in order to accurately detect colonies, the threshold (tone value) when binarizing each pixel of the medium image by the profile is the culture time. If the difference in brightness between the pixels that make up the colony and the pixels that make up the culture medium or specimen is small depending on the type of fungus or specimen, the threshold for binarization is the brightness It is set smaller than when the difference is large.
  • the threshold for binarization when the dilution factor is large or when there is a high possibility of mixing non-objects such as dust without using a filter, the threshold for binarization is high and the dilution factor is low. In some cases or when a filter is used, the threshold is set small.
  • the colony detection determination unit 423 (1) Based on whether image filter processing including expansion processing and reduction processing for reducing image filter noise is performed, and (2) Based on a connection method and its parameters when connecting adjacent colony pixels and performing labeling to give an ID, Detect colony candidates.
  • the colony detection determination unit 423 regarding the connection of colony pixels, based on a range that should be determined to be the same as the feature amount related to the color of each colony pixel specified by the profile, the same colony candidate for adjacent colony pixels Concatenate those determined to be.
  • the colony detection determination unit 423 connects adjacent colony pixels to form a colony pixel group, and uses the color characteristics for each colony pixel group or the barycentric position on the medium image to form the same colony pixel Connect them together.
  • the expansion processing is one of image filter processing, and is processing that replaces all pixels adjacent to the colony pixel with colony pixels when other colony pixels exist adjacent to each colony pixel. .
  • the contraction process is a process of replacing all pixels adjacent to the colony pixel with non-colony pixels when there is a non-detected pixel adjacent to the colony pixel (hereinafter referred to as “non-colony pixel”). .
  • the colony detection determination unit 423 temporarily stores predetermined information such as the arrangement position, color characteristics, or barycentric position of the colony pixel on the medium image for each extracted colony candidate in the ROM / RAM 440.
  • the colony detection determination unit 423 (1) For each detected colony candidate, a likelihood indicating a colony-likeness is calculated based on feature quantities such as color characteristics, roundness, shape, and size, and the likelihood has a predetermined condition such as a threshold value or more. Specify colony candidates as colonies, or (2) For each detected colony candidate, a multidimensional histogram is formed on the basis of a feature quantity of two or more colors of colony pixels belonging to each colony candidate, and a coordinate system defined by the multidimension (feature quantity space) ), It is specified whether or not it is a colony with reference to a coordinate position defined based on each feature amount.
  • the colony detection determination unit 423 (1) (1A) the average value of the feature values regarding the color of each colony pixel belonging to the colony candidate, (1B) The roundness of the colony based on the coordinates of the center of gravity of the colony candidate, and (1C) Edge strength indicating the strength of the edge at the boundary of colony candidates, And the likelihood of each colony candidate is calculated based on these.
  • the distance Ra from the coordinates of the center of gravity of the colony candidate to the outermost colony pixel at the shortest distance on the film type medium 60 and the longest distance on the film type medium 60 from the coordinates of the center of gravity of the colony candidate The value “M” calculated according to (Equation 1) based on the ratio to the distance Rb to the outermost colony pixel in FIG. 5 or the area “S” of the colony and the length “L” of the outer periphery is used.
  • the edge strength the average of the differences in luminance, saturation, or RGB gradation value between each outermost colony pixel and a non-colony pixel adjacent to the outermost colony pixel is used.
  • the colony detection determination unit 423 for (2), each gradation value of RGB of each colony pixel of the colony candidate defined by the profile, luminance value, saturation, these two or more color components, or these Each colony pixel is multi-valued into n (“n” is an integer of 2 or more) based on a feature amount such as a feature amount newly calculated by a statistical method based on the value of each color component value.
  • a color distribution (histogram) having a bin number “n” obtained by counting the number of colony pixels (that is, the number of colony pixels having gradation values belonging to the range) is generated.
  • the colony detection determination unit 423 normalizes the number of colony pixels of each bin of the generated histogram (calculates a ratio based on the number of all colony pixels) and specifies each as a feature amount. That is, the colony detection determination unit 423 specifies the normalized number of colony pixels in n bins as a feature amount.
  • the colony detection determination unit 423 determines a coordinate position based on the specified feature amount in a multi-dimensional feature amount space, and the coordinate position is defined as a colony by the profile. It is determined whether or not it belongs to the region, and whether or not the colony candidate is a colony is specified based on the result.
  • the threshold of likelihood for specifying the colony by the profile is set smaller than when the culture time is long.
  • the likelihood threshold is the brightness difference. Is set smaller than when the colony is large or the colony tends to be circular.
  • the threshold value is set small.
  • the colony detection determination unit 423 counts the identified number as the number of colonies.
  • the colony detection determination unit 423 may calculate a connection cost when adjacent colony candidates are connected, and may connect them as the same colony candidate when the connection cost is equal to or less than a threshold value.
  • the threshold value is included in the profile information, and the colony detection determination unit 423 may calculate the connection cost by setting the threshold value.
  • the colony detection determination unit 423 determines whether the specimen of each film-type medium 60 is abnormal or normal based on the threshold set based on the acquired profile and the detected number of colonies (that is, the specimen Execute pass / fail judgment).
  • the threshold of the number of bacteria when the culture time is short, in order to detect colonies strictly, the threshold of the number of bacteria is set by the profile to be smaller than that when the culture time is long. In some cases, when the detection of a certain number of bacteria does not cause a problem, the threshold value of the number of bacteria is set to be smaller than that when a large number of bacteria is detected.
  • the threshold of the number of bacteria is high and the dilution factor is low or the filter Is used, the threshold value is set small.
  • the pass / fail determination of each film-type medium 60 is used in the process determination, the line determination, and the lot determination, the medium determination is executed in association with these determinations.
  • the method of pass / fail determination of each film type culture medium 60 in process determination, line determination, and lot determination will be described later.
  • the colony detection determination unit 423 stores the detected colony pixel, the detected colony candidate, the specified colony, the counted number of colonies, and the medium determination result in the medium information DB 401 via the registration processing unit 421 as medium information.
  • the lot determination processing unit 424 of the present embodiment analyzes data on hygiene management in the designated lot, work line 80 or process based on the instructions of the administrator input to the administrator terminal device 20 (ie, pass / fail determination). Execute.
  • the lot determination processing unit 424 for each lot, line information of the work line 80 used for the corresponding lot, and medium information registered in association with the corresponding work line 80 (at least the number of colonies). Based on the above, it is determined whether the food group manufactured or inspected under the same condition has a predetermined condition.
  • the lot determination processing unit 424 determines whether the work line 80 included in the corresponding lot and the processes belonging to the work line 80 are abnormal or normal in terms of hygiene management. 80 line determinations (that is, pass / fail determination for hygiene management) and process determinations for each process (that is, determination of pass / fail for hygiene management) are performed, and lot determination is performed using the process determination and line determination.
  • the lot determination processing unit 424 when the lot determination is instructed, the lot determination processing unit 424, for example, the number of colonies of the film-type medium 60 in the medium information that has passed a predetermined culture inspection time such as 24 hours or 48 hours from the start of culture, Based on the medium information of the pass / fail determination of the specimen, the pass / fail of hygienic management of the process is determined, and the pass / fail of hygiene management of the work line 80 is determined based on the pass / fail determination of the process included in the corresponding work line 80. Based on the pass / fail determination of the work line 80 included in the corresponding lot, the pass / fail in sanitary management of the lot is determined.
  • the lot determination processing unit 424 of the present embodiment may execute not only lot determination but also process determination or line determination as data analysis.
  • the lot determination processing unit 424 has a culture start time at the same timing, a culture examination time at the same timing, a culture start time having a time within a preset time from the first time to the second time, or a preset first time.
  • the culture start time or the culture test time has a predetermined condition, such as having a culture test time having a time within the time from the first time to the second time, and is registered in association with the same process in the same lot.
  • Each process determination is performed based on the culture medium information of the plurality of film-type culture media 60 that has been performed. That is, the lot determination processing unit 424 executes each process determination by comprehensive determination based on each medium determination result of the plurality of film-type mediums 60.
  • the lot determination processing unit 424 displays the medium determination results of the corresponding film-type medium 60 (that is, all the medium determination results registered in the medium information having the process ID 71b of the process to be subjected to the process determination).
  • the process judgment of the process to be judged is judged to be acceptable, and if not, the judgment should be made The process judgment of the process is judged as rejected.
  • the lot determination processing unit 424 determines that the conditions for all the film-type culture media 60 when the acceptable film-type culture media 60 are a predetermined ratio (60%) or more in the individual film-type culture media 60 in the corresponding process ( The number of colonies is equal to or less than a certain number), or the condition that the number of colonies of each film-type medium 60 corresponds (the average number of colonies of a single film-type medium 60 is equal to or less than a certain number). In this case, the corresponding process is determined to be acceptable.
  • the lot determination processing unit 424 of the present embodiment can change conditions depending on the type of specimen, the type of bacteria to be detected, etc., and can be set by an administrator or provided as part of a program.
  • the lot determination processing unit 424 performs line determination for each work line 80 based on the pass / fail determination for hygiene management of a plurality of processes that are determined at a predetermined timing and registered in association with the corresponding line information. To do. That is, the lot determination processing unit 424 performs line determination by comprehensive determination based on the determination results of a plurality of processes of the same lot determined at a predetermined timing.
  • the lot determination processing unit 424 is determined at the same timing or a timing satisfying a predetermined condition, and has the line ID of the work line 80 to be subjected to line determination as described above.
  • the line determination of the work line 80 to be determined is determined to be acceptable, and the condition is not satisfied
  • the line determination of the work line 80 to be determined is determined to be unacceptable.
  • the lot determination processing unit 424 passes the line determination of the work line 80 to be determined.
  • the lot determination processing unit 424 executes the lot determination for each lot based on the hygiene management pass / fail determination of the plurality of work lines 80 that are determined at a predetermined timing and registered in association with the corresponding lot information. To do. That is, the lot determination processing unit 424 executes lot determination by comprehensive determination based on the determination results of the plurality of work lines 80.
  • the lot determination processing unit 424 performs lot determination based on the pass / fail determination in the work line 80 having the lot ID of the lot for which lot determination is to be performed.
  • the lot determination processing unit 424 determines that the lot determination of the lot to be determined is acceptable when the line determination is successful in 60% of the work lines 80 having the lot ID, and the three work lines 80 determine the lot to be determined. Assume that a lot ID is included. In this case, when the first work line 80 is “pass”, the second work line 80 is “pass”, and the third work line 80 is “fail”, the lot determination processing unit 424 determines the lot to be determined. It is determined that the lot determination is acceptable.
  • the lot may be determined by dividing the time every predetermined time (for example, 8 hours or 12 hours). In this case, when the lot determination is performed while the work is suspended, a dummy result may be used, or it may be determined that the work is being suspended.
  • the lot determination processing unit 424 can perform determinations and data analysis other than those described above using the culture medium information, line information, lot information, and work management DB 404, and the determination result or data analysis via the report processing unit 425.
  • the result can be provided to the administrator terminal device 20 so that the administrator can view the result.
  • the lot determination processing unit 424 checks the reliability of the sample cultured in each film-type medium 60, that is, checks whether or not an inspection error has occurred.
  • the medium information in the medium ID is subjected to a predetermined analysis of the medium information or the medium image data in time series from the culture start time to the end of the culture.
  • the lot determination processing unit 424 has a specific culture medium ID (same culture medium ID) 66 based on an instruction from the administrator received via the administrator terminal device 20, and starts from the culture start time to a predetermined time. Are extracted together with the medium image data. Then, the lot determination processing unit 424 performs a predetermined analysis on each medium information and each image data along a predetermined time series, or aggregates each medium information and each image data along a time series. In addition, the lot determination processing unit 424 causes the report processing unit 425 to generate the analysis result or the aggregation result as browsing data having a predetermined data format, and allows the administrator to browse the generated browsing data. 20 to provide.
  • a specific culture medium ID standard culture medium ID
  • the lot determination processing unit 424 causes the report processing unit 425 to generate the analysis result or the aggregation result as browsing data having a predetermined data format, and allows the administrator to browse the generated browsing data. 20 to provide.
  • the lot determination processing unit 424 is linked to the report processing unit 425, and at this time, based on an instruction from the manager terminal device 20, each medium information in one medium ID 66 is used for legal hygiene management reporting. May be made individually viewable.
  • the lot determination processing unit 424 may have a search function for medium information, line information, lot information, or work management information. Specifically, the lot determination processing unit 424 uses the lot ID (instruction ID 71a), the work line ID, the process ID 71b, the work ID 71, the culture start time, the lot work date and time, the culture medium ID 66, the specimen type, and the like as search keys.
  • the database 400 may be searched, and the report processing unit 425 may be linked to generate data that allows the administrator to view the corresponding medium information and various types of information.
  • the report processing unit 425 is configured to search for medium information (medium image data, number of colonies and medium determination result), process information, and line specified by a search based on an instruction from the administrator received via the administrator terminal device 20. It is also possible to provide information or information on lots in a predetermined report format. That is, the report processing unit 425 is configured to be able to provide various information as evidence or reports in legal inspection reports and other cases.
  • the report processing unit 425 performs data analysis related to hygiene management in the designated lot, work line 80 or process based on the administrator's instruction input to the administrator terminal device 20 and template data having a predetermined report format.
  • An analysis result (that is, a determination result of pass / fail determination) is generated as report data, and the generated report data is provided to the administrator terminal device 20 so as to be viewable by the administrator via the communication control unit 410.
  • the report processing unit 425 extracts necessary information from the medium information, the line information, and the lot information such as the medium image data, the number of colonies, or the medium determination result, and the extracted information and the lot determination processing unit 424. Based on the analysis result obtained by the above, report data is generated while assigning each information to the template data.
  • the report processing unit 425 is configured to generate predetermined report data or browsing data in accordance with various analyzes or searches and provide the data to the administrator terminal device 20. .
  • the report processing unit 425 can also provide medium information, process information, line information, or lot-related information specified by the search in a predetermined report format based on the received administrator's instruction.
  • it is configured so that various information can be provided as evidence or reports in statutory inspection reports and other cases.
  • FIG. 6 is a flowchart showing the culture information registration processing operation in the portable communication terminal device 10 of the present embodiment.
  • the medium ID or instruction ID is attached to the film-type medium 60 and the work instruction sheet 70 by a two-dimensional barcode.
  • the number of colonies in each film-type medium 60 is detected at the time of registration in the medium information database 400, and the portable communication terminal device 10 uses RGB for capturing a medium image. It is assumed that calibration for the gradation value or the luminance value is performed.
  • the application control unit 120 detects an activation instruction for the culture medium registration application via the operation unit 170 (step S101)
  • the application control unit 120 reads the culture medium registration application from the application storage unit 101 and activates it (Ste S102).
  • the application control unit 120 performs initialization of the work memory and other necessary processes under the control of the mobile terminal management control unit 190.
  • the application control unit 120 displays an image for interlocking with the display control unit 161 to select whether the initial registration of the film-type medium 60 or the post-culture registration after a predetermined time has elapsed from the start of the culture. It is displayed on the unit 160 and waits for its input (step S103).
  • step S104 when detecting an input of a selection instruction by the operation unit 170 (step S104), the application control unit 120 determines whether the registration is initial registration or post-culture registration, and sets the result in flag information (step S105).
  • the application control unit 120 causes the display unit 160 to display a screen that prompts the imaging of the film-type medium 60, and waits for imaging by the image data generation unit 110 in conjunction with the operation unit 170 (step S106). At this time, the application control unit 120 causes a predetermined image to be superimposed on the display unit 160 in order to align the medium image to be acquired.
  • the image data generation unit 110 causes the medium ID 66 to be detected.
  • the imaged medium image data of the film type medium 60 is acquired and stored in the image data storage unit 102 while giving a predetermined image ID (step S108).
  • the application control unit 120 acquires the current time as the imaging time of the medium image data from the timer 180, and stores it in the image data storage unit 102 as metadata in association with the medium image data (step S109).
  • the application control unit 120 analyzes the two-dimensional barcode formed in a predetermined area of the acquired imaged culture medium image data, acquires the culture medium ID 66, and stores the image data in association with the culture medium image data.
  • the information is stored in the unit 102 (step S110).
  • the application control unit 120 causes the display unit 160 to display a screen for acquiring the instruction sheet image data together with the instruction sheet ID 71a and the process ID 71b in conjunction with the display control unit 161, and waits for acquisition of the instruction sheet image data. (Step S111).
  • the application control unit 120 analyzes the two-dimensional barcode formed in the predetermined region of the captured instruction image data and the medium image data, acquires the work ID 71, and corresponds to the instruction image data
  • the image data is stored in the image data storage unit 102 (step S114).
  • the application control unit 120 acquires the current time as the imaging time of the medium image data from the timer 180, and in conjunction with the display control unit 161 and the operation unit 170, the application control unit 120 acquires the acquired instruction image data, work ID 71, The medium image data, medium ID 66, and imaging time are confirmed (step S115).
  • the application control unit 120 may re-acquire the culture image data based on an instruction from the operator when the acquired medium information is confirmed by the operator. At this time, the application control unit 120 may be able to correct the medium image data by the operator in conjunction with the display control unit 161 and the operation unit 170.
  • the application control unit 120 uses the terminal ID and the work ID 71 as well as the acquired medium image data and its metadata, and flag information indicating whether initial registration or post-culture registration as medium information. Then, the data is transmitted to the server device 40 via the network communication unit 130 (step S116), and the registration process of the portable communication terminal device 10 is terminated.
  • the network communication unit 130 executes login for accessing the server device 40 based on the terminal ID or the input ID and password, and the server device 40 is completed after the login is completed. Communication line is established and medium information is transmitted.
  • FIG. 7 is a flowchart showing the culture information registration processing operation in the server device 40 of this embodiment.
  • the registration processing unit 421 is based on the flag information included in the received culture medium information. Whether the registration is initial registration or post-culture registration is determined (step S301).
  • step S302 If the registration processing unit 421 determines that it is not initial registration, that is, if it is determined to be post-culture registration, step S304 is performed. Move on to processing.
  • the registration processing unit 421 registers the culture medium information in the culture medium information DB 401 based on the identified work ID 71 (step S303), and ends this operation.
  • the culture start time is stored in advance in the work management DB 404
  • the registration processing unit 421 registers the imaging time included in the received medium information as the culture start time in the work management DB 404 in the process of step S303. Also good.
  • the registration processing unit 421 determines that the registration is after culture, it extracts and specifies the work ID 71 included in the culture medium information (step S304), and registers the culture medium information based on the imaging time included in the culture medium information.
  • the culture examination time to be specified is specified (step S305).
  • the registration processing unit 421 causes the colony detection determination unit 423 to acquire predetermined inspection condition information based on the acquired medium information and the specified work ID (step S306), and causes the acquired inspection condition information to be acquired. Based on this, the profile indicating the inspection standard is specified (step S307).
  • the colony detection determination unit 423 performs image analysis on the received medium image data based on the specified profile, and extracts the feature amount (luminance value) of each pixel constituting the medium image (In step S308), the number of colonies generated in the specimen imaged based on the feature amount of each pixel constituting the culture medium image is detected and the number thereof is detected (step S309).
  • the colony detection determination unit 423 determines whether the detected number of colonies satisfies a predetermined condition based on the specified profile (step S310). That is, the colony detection determination unit 423 determines that it is acceptable (normal) when the predetermined condition is satisfied, and determines that it is unacceptable (abnormal) when the predetermined condition is not satisfied.
  • the colony detection determination unit 423 is linked to the portable communication terminal device 10 instead of the automatic registration described above, and displays the detected number of colonies and the result of the pass / fail determination on the display unit 160 of the portable communication terminal device 10. The user may be allowed to view and confirm.
  • the registration processing unit 421 specifies culture start time information using the culture image data, the recognized medium ID 66, the determined pass / fail, the number of detected colonies, and the imaging time included in the medium information as the culture start time.
  • the work ID 71 is associated and registered in the culture medium information DB 401 (step S311), and this operation ends.
  • the registration processing unit 421 displays line information and information to be registered on the display unit 160 of the portable communication terminal device 10, and based on a user instruction input via the portable communication terminal device 10, the culture medium Information or the like may be registered.
  • the registration processing unit 421 receives the reception in the process of step S201.
  • the medium ID 66 imaged on each predetermined area of the medium image data thus obtained is analyzed, and the medium ID 66 is recognized and acquired.
  • the server device 40 also recognizes the work ID 71 in the instruction image data.
  • the culture medium information acquired by the portable communication terminal apparatus 10 is recorded in the culture medium information DB 401 and then executed. May be.
  • Modification 1 In this embodiment, instead of performing the culture information registration process using a portable communication terminal device 10 such as a tablet information terminal device, a smartphone, or a mobile phone, a laptop or desktop personal computer is used.
  • the registration process of the culture medium information may be realized by a computer and an image input device such as a scanner, a digital camera, or a smartphone.
  • the inspection condition information acquired based on the culture medium information and the work ID is displayed on the administrator terminal device 20, and the subsequent processing is executed based on the administrator's instruction or correction instruction.
  • the portable communication terminal device 10, the administrator terminal device 20, and the server device 40 may be installed or used in the same site, or each may be installed in a remote place such as outside the country or remotely. It may be used on the ground and each process described above may be executed. However, it is assumed that the portable communication terminal device 10 is used in the same lot.
  • Modification 4 In the present embodiment, registration of the medium information in the specimen cultured on the film type medium 60 using the film type medium 60 is performed. However, the bacteria cultured on the agar medium using the agar medium and the bacteria Registration of medium information in a specimen may be executed.
  • the culture medium ID 66 may be input by an operator's operation input.
  • the application control unit 120 interlocks with the display control unit 161 and the operation unit 170, and allows the operator to manually input the culture medium ID 66 directly using the operation unit 170.
  • the application control unit 120 controls the display control unit 161 and the operation unit 170 to select a display item such as a pull-down menu by clicking or touch selection, and causes the operator to input the medium ID 66.
  • Modification 6 In the present embodiment, various processes are executed by the single server device 40, but various processes may be executed by a server system including a plurality of server devices 40.
  • the colony is inspected at the timing (culture time from the start of culture to the test time). Fluctuating conditions, conditions determined based on the specimen such as the type of specimen placed on the culture medium, or culture conditions indicating the presence or absence of a filter when extracting the diluent or specimen used for the test and placing it on the culture medium, etc.
  • an accurate inspection can be performed even when the culture environment of the colonies to be cultured is different, and as a result, the inspection result as the medium information is accurately detected. Can be registered.
  • hygiene management related to food using the film-type culture medium 60 can be analyzed by electronic data. At the same time, the analysis result can be provided electronically.
  • the present invention is not limited to the food, and can be applied to products that are provided to all living organisms including drugs and drugs. .
  • the present invention can be used in food hygiene management systems and the like.
  • DESCRIPTION OF SYMBOLS S ... Hygiene management system 10 Portable communication terminal device 20 ... Manager terminal device 30 ... Network 40 ... Server device 60 ... Film type culture medium 70 ... Work instruction sheet 80 ... Work line 100 ... Data storage part 101 ... Application storage part 102 Image data storage unit 110 Image data generation unit 120 Application control unit 130 Network communication unit 140 Short-range wireless communication interface 150 Current position detection unit 160 Display unit 170 Operation unit 180 Timer 190 Mobile device management Control unit 210 ... Communication control unit 220 ... Label issuance processing unit 230 ... Administrator terminal management control unit 250 ... Timer 260 ... Display unit 261 ... Display control unit 400 ... Database 401 ... Medium information DB 402 ... Line information DB 403 ...
  • Lot information DB 404 ... Work management DB 405 ... Profile DB 410 ... Communication control unit 420 ... Data processing unit 421 ... Registration processing unit 423 ... Colony detection determination unit 424 ... Lot determination processing unit 425 ... Report processing unit 430 ... Server management control unit

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Abstract

La présente invention porte sur un système d'enregistrement d'informations de milieu de culture ou analogue permettant d'exécuter précisément une inspection d'un milieu de culture même lorsqu'une condition concernant un environnement de culture pour une colonie change durant l'inspection et, en résultat, d'enregistrer précisément le résultat de l'inspection à titre d'informations de milieu de culture. Un système de maintien de l'hygiène (S) est configuré de manière que des informations de condition d'inspection, indiquant une condition d'inspection concernant un environnement de culture pour une colonie dont une culture est faite dans un milieu de culture du type film (60), soit acquises lorsqu'une inspection prescrite est exécutée suivant une acquisition de données d'image de milieu de culture comprenant une image de milieu de culture obtenue par capture et formation d'image du milieu de culture du type film (60), un critère de détection de colonie pour la colonie dont une culture est faite dans le milieu de culture du type film (60) est spécifié sur la base des informations de condition d'inspection acquises, l'inspection prescrite est exécutée par analyse de l'image de milieu de culture sur la base du critère de détection de colonie spécifié, et le résultat de l'inspection prescrite est enregistré dans une base de données (400).
PCT/JP2014/052645 2013-02-07 2014-02-05 Dispositif d'inspection de milieu de culture, système d'enregistrement d'informations de milieu de culture, système de maintien de l'hygiène et programme WO2014123143A1 (fr)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010104301A (ja) * 2008-10-30 2010-05-13 Eiken Chem Co Ltd 微生物の検出方法、装置およびプログラム
JP2011512845A (ja) * 2008-03-04 2011-04-28 スリーエム イノベイティブ プロパティズ カンパニー 測定された製造特性に基づく生物学的増殖培地の処理
JP2011212013A (ja) * 2010-03-17 2011-10-27 Elmex Ltd フィルム型培地による微生物検査方法およびこの微生物検査方法に用いる画像取込ツ−ル
JP2011243188A (ja) * 2010-04-23 2011-12-01 Nagoya Univ 画像処理装置、細胞分類装置、インキュベータ、画像処理方法、細胞分類方法、画像処理プログラムおよび細胞分類プログラム
JP2012135240A (ja) * 2010-12-27 2012-07-19 Hitachi High-Technologies Corp 細菌コロニー同定装置およびその方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011512845A (ja) * 2008-03-04 2011-04-28 スリーエム イノベイティブ プロパティズ カンパニー 測定された製造特性に基づく生物学的増殖培地の処理
JP2010104301A (ja) * 2008-10-30 2010-05-13 Eiken Chem Co Ltd 微生物の検出方法、装置およびプログラム
JP2011212013A (ja) * 2010-03-17 2011-10-27 Elmex Ltd フィルム型培地による微生物検査方法およびこの微生物検査方法に用いる画像取込ツ−ル
JP2011243188A (ja) * 2010-04-23 2011-12-01 Nagoya Univ 画像処理装置、細胞分類装置、インキュベータ、画像処理方法、細胞分類方法、画像処理プログラムおよび細胞分類プログラム
JP2012135240A (ja) * 2010-12-27 2012-07-19 Hitachi High-Technologies Corp 細菌コロニー同定装置およびその方法

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