WO2014119875A1 - Pharmaceutical composition for cough relief or discharge of phlegm, containing hot water extract of dendropanax morbiferus h.lev. leaves - Google Patents

Pharmaceutical composition for cough relief or discharge of phlegm, containing hot water extract of dendropanax morbiferus h.lev. leaves Download PDF

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WO2014119875A1
WO2014119875A1 PCT/KR2014/000680 KR2014000680W WO2014119875A1 WO 2014119875 A1 WO2014119875 A1 WO 2014119875A1 KR 2014000680 W KR2014000680 W KR 2014000680W WO 2014119875 A1 WO2014119875 A1 WO 2014119875A1
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Prior art keywords
extract
pharmaceutical composition
expectorant
antitussive
hwangchil
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PCT/KR2014/000680
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French (fr)
Korean (ko)
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최철웅
김재용
반상오
김희숙
강후원
장욱진
설희진
이규옥
이동욱
김선오
김재갑
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재단법인 전남생물산업진흥원
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Publication of WO2014119875A1 publication Critical patent/WO2014119875A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/10—Expectorants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/14—Antitussive agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine

Definitions

  • the present invention relates to a composition for antitussive or expectorant using natural extracts derived from hwangchil as an active ingredient, and more specifically, using hwangchil leaf hot water extract as a natural ingredient as an active ingredient and can be safely used without toxicity and side effects.
  • the present invention relates to a pharmaceutical composition for antitussives or expectorants, which contains a yellow lacquer extract capable of relieving cough (jinhae) or sputum removal (sputum) without side effects even after taking hot water extract for a long time.
  • Hwangchil-tree is a biological classification belonging to the flowering plants, dicotyledonous, mountain-shaped tree, arboraceae family, and has economic value as a traditional paint of Korea, and the southwest coast of Jeollanam-do and Halla-san of Jeju-do, including Wando, Haenam and Bogildo It is an evergreen broad-leaved arboreous tree native to the area.
  • the hwangchil trees reach a height of 15 m, young branches are green and hairless.
  • the leaves of the Hwangchil tree are shifted and egg-shaped or oval, and the flowers bloom in light yellow-green in June and are bisexual.
  • the fruit of the Hwangchil-tree is oval, opened in black in October.
  • the essence of the yellow lacquer tree, which is used for the traditional craft, Hwangchil, is collected in August to September.
  • Hwangchil has been used in furniture, leather, cloth, metal and paper since the Three Kingdoms since it was a transparent natural paint. Especially, the hwangchil woodwork has the advantage of making woodwork more bright by vividly bringing up the wood texture of the base. Widely used in the woodworking field.
  • Hwangchil's components consist of 66.7% of non-volatile components, 10.8% of aromatic components, 8.1% of moisture, and 14.4% of solid components, which are the paint components that form the golden coating.
  • the aromatic component consists mainly of sesquiterpenes, ⁇ -cubebene, ⁇ -selinene, and ⁇ -cadinene, which are known to exhibit sedative and tonic effects on the nervous system. .
  • the main ingredient is fermentation broth obtained by fermenting with lactic acid bacteria using only extract of jellyfish, a tonic animal designated as an ecological disturbance by destroying marine ecological environment.
  • the present invention relates to a health functional food composition having a therapeutic effect and an improvement effect using apoptosis-inducing action of killing normal cancer cells, and a method of manufacturing the same,
  • Korean Unexamined Patent Publication No. 10-2011-00801387 a composition additive for hair and a method for manufacturing the same, comprising a extract of Hilchi chinensis which provides an effect of protecting skin and scalp by preventing skin irritation and dermatitis
  • -2007-0098529 discloses a method for preparing functional yellow lacquer having a bactericidal and detoxifying function for producing a functional yellow lacquer having a bactericidal and detoxifying function and an antiseptic function.
  • Korean Laid-Open Patent Publication No. 1998-026683 discloses a solvent composition for a yellow lacquer paint, which is a golden natural paint prepared by extracting sap from a yellow lacquer tree, but in the prior art, hot water of a yellow lacquer leaf as a natural raw material.
  • the extract By using the extract as an active ingredient, it can be safely used without toxicity and side effects, and it contains Hwangchil leaf hydrothermal extract to enable cough relief (jinhae) or sputum removal (sputum) without side effects even after long-term use of Hwangchil leaf hydrothermal extract. It differs from pharmaceutical compositions for antitussive or expectorant.
  • the yellow lacquer leaves (based on fresh leaves), which sell for 12,000 to 25,000 won per kg, compared to the sap sold for 90,000 to 100,000 won per g. Able to know.
  • the present invention can be safely used without toxic and side effects by using Hwangchil leaf hydrothermal extract, which is an economical natural material, as an active ingredient, which is superior to Hwangchil sap.
  • Hwangchil leaf hydrothermal extract which is an economical natural material, as an active ingredient, which is superior to Hwangchil sap.
  • the present invention relates to a composition for antitussive or expectorant containing Hwangchil leaf hydrothermal extract that effectively acts on antitussive or expectorant activity. And it is possible to manufacture a functional health food having a high value, and to provide a safe antitussive or expectorant composition without side effects even when taken for a long time.
  • the present invention provides a pharmaceutical composition for antitussive or expectorant, comprising hwangchil leaf extract or non-polar soluble extract as an active ingredient.
  • the crude yellow leaf extract may be an extract available in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof, and hexane and chloroform as non-polar solvents in the crude yellow leaf extract extracted using the extracting solvent.
  • Dichloromethane and ethyl acetate can be fractionated using any one of the fractions.
  • the pharmaceutical composition for antitussive or expectorant comprising an extract available in any one of the water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof as an active ingredient.
  • the daily dosage of the extract may be consumed in an amount of 10 to 2000 mg / kg per kg body weight.
  • the pharmaceutical composition for antitussive or expectorant characterized in that the crude extract, which comprises a fraction using any one of hexane, chloroform, dichloromethane and ethyl acetate as a fraction solvent as a non-polar solvent as an active ingredient. It may be included in an amount of%, the daily dosage of the extract may be administered in an amount of 1 to 1000 mg / kg fraction per kg body weight.
  • the fraction is fractionated with chloroform of the hydrothermal extract of Hwangchil leaves as an active ingredient, the fraction comprises 0.01 to 99.9% by weight of the total composition, the daily dose of the pharmaceutical composition for antitussive or expectorant Is the amount of the fraction contained 1 to 1000 mg / kg body weight.
  • the composition is used in the pharmaceutical composition for the prevention or treatment of respiratory diseases
  • the respiratory diseases are emphysema, chronic bronchitis, bronchial adenomas, isolated pulmonary nodules, pulmonary tuberculosis, empyema, lung abscess, cold, flu or histiocytosis of the lung
  • the composition is characterized in that it is used as a pharmaceutical composition for the prevention or treatment of respiratory diseases formulated in powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, transdermals, suppositories or sterile injectable solutions.
  • the present invention can be used to remove cough (jinhae) or sputum (sputum) without side effects even after long-term use by using a natural Hwangchil leaf hydrothermal extract as an active ingredient, chloroform fraction of the natural Hwangchil leaf hydrothermal extract as an active ingredient By using it for a long time, there is a remarkable effect of eliminating cough (jinhae) or sputum removal (sputum) without side effects.
  • 1 is a photograph showing the yellow chil leaves.
  • Figure 2 shows the extraction and fractional schematic of the Hwangchil leaf hydrothermal extract.
  • Figure 3 shows the results of the measurement of the mucus secretion in the airway for the hot-water extract of yellow chile.
  • Figure 4 shows the results of the measurement of mucus secretion in the airway for the hot water extract of the yellow lacquer extract and methanol extract.
  • Figure 5 shows the results of the measurement of the mucus secretion in the airway for the solvent-specific fractions of the yellow-leaf leaf hydrothermal extract.
  • Figure 6 shows the results of allergy in the skin sensitization test animal model of the solvent-specific fractions of the Hwangchil hydrothermal extract and Hwangchil hydrothermal extract.
  • the present invention provides a pharmaceutical composition for antitussive or expectoration comprising crude yellow leaf extract or non-polar soluble extract as an active ingredient.
  • the crude yellow leaf extract may be an extract available in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof, and hexane and chloroform as non-polar solvents in the crude yellow leaf extract extracted using the extracting solvent.
  • Dichloromethane and ethyl acetate can be fractionated using any one of the fractions.
  • the extract may be included as an amount of 0.01 to 99.9% by weight of the total composition to serve as an antitussive or expectorant beverage, the daily dosage of the extract is to be drinking in an amount of 10 to 2000mg / kg per kg body weight Can be.
  • It comprises a fraction fractionated with chloroform of the Hwangchil leaf hydrothermal extract as an active ingredient, the fraction comprises 0.01 to 99.9% by weight of the total composition, the daily dose of the antitussive or expectorant pharmaceutical composition is 1 to the fraction 1000 mg / kg body weight content.
  • Extraction method of the composition for Jinhae or expectorant is a step of washing the hot-chil leaves with distilled water and then mixed with distilled water to extract hot water; Lyophilizing the filtered and concentrated extract; An extraction method is provided, including filtration and concentration of the extract of the above step by dissolving in a fractional solvent to obtain a fraction by filtration, concentration, and lyophilization.
  • the extracts are also used in pharmaceutical compositions for the prevention or treatment of respiratory diseases, which are formulated as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, transdermals, suppositories or sterile injectable solutions. It is characterized in that it is used in the pharmaceutical composition for the prevention or treatment of respiratory diseases.
  • Figure 2 shows the process of obtaining a fraction by the yellow-leaf leaf hydrothermal extract and the organic solvent.
  • 1 kg of dried yellow lacquer was washed with distilled water, 20 L of distilled water was added thereto, and heated and extracted at 100 ° C. for 3 hours using an electric bath.
  • the extracted solution was filtered through a 400 mesh filter cloth and then concentrated using a vacuum concentrator. After filtration, the residue was extracted, filtered and concentrated under reduced pressure two more times using the same amount of distilled water.
  • the concentrated hot water extract was lyophilized in a freeze dryer. After drying, 279 g (24.9%) of yellow-hot leaf hydrothermal extract was obtained.
  • Butanol soluble fraction and insoluble fraction were separated by adding 5 L of butanol to the ethyl acetate insoluble fraction (aqueous layer), and the same process was repeated three times for the butanol insoluble layer to collect butanol insoluble fraction and soluble fraction.
  • the hexane soluble fraction, the chloroform soluble fraction, the ethyl acetate soluble fraction, and the butanol soluble fraction were concentrated under reduced pressure in 249 g of Hwangchil leaf hydrothermal extract, and then freeze-dried to obtain 0.096 g (0.04%) of hexane fraction, 0.57 g (0.84%) of chloroform fraction, and ethyl acetate. 4.04 g (1.63%) fraction, 54.74 g (10.28%) butanol fraction and 168.64 g (67.84%) water fraction were used as samples.
  • mice Male ICR mice were divided into three groups: normal, positive control, and sample administration groups.
  • the normal group was orally administered 100 ⁇ L of saline, and the positive control group was orally administered 200 ⁇ L of ammonium chloride (NH 4 Cl) at a concentration of 1000 mg / kg.
  • NH 4 Cl ammonium chloride
  • the experimental group was orally administered 100 ⁇ L at 50, 100 and 200 mg / kg concentration of the Hwangchil leaf hot water extract, and the sample was dissolved in saline, and NH4Cl was dissolved in tertiary distilled water. Each group was intraperitoneally injected with 0.2 ml of phenol red 10 mg / kg after 1 hour of oral administration.
  • mice in each group were anesthetized using diethyl ether (dimethylether) after 30 minutes, and the abdominal aorta was cut and bleeded, and then the entire trachea was excised.
  • the separated organs were placed in 1 ml of saline, and the tissues were washed and vortexed for 30 minutes, and then the washing solution was centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml was taken from the separated supernatant, and 0.1 ml of 1M NaOH was added to the solution to develop color. This solution was measured at absorbance 546 nm to determine the phenol red extracted from the airways. The amount of phenol red to airway was determined by dividing the weight by airway weight.
  • the content of phenol red per 1 g of airway was 2.436 ⁇ g / g in the normal group, 3.476 ⁇ g / g in the NH4Cl group used as the positive control group, and the experimental group of Hwangchil leaves 2.958 (21.4%), 3.108 (27.6%), and 3.105 ⁇ g / g (27%) were obtained in the 50, 100 and 200 mg / kg groups. It was confirmed that the extract of Hwangchil leaves exhibited excellent activity (sputum activity) by increasing phenol red content, that is, increasing mucus secretion, in a concentration-dependent manner.
  • mice Male SD Rat mice were divided into three groups: normal, control, and sample administration.
  • the normal group was orally administered 500 ⁇ L of saline, and the positive control group was orally administered 1 ml of ammonium chloride (NH 4 Cl) at a concentration of 1000 mg / kg.
  • NH 4 Cl ammonium chloride
  • the experimental group was orally administered Hwangchil leaf hydrothermal extract and 500ml L of hwangchil leaf methanol extract at a concentration of 200mg / kg, respectively, the sample was prepared by dissolving in saline, NH4Cl used in the positive control was prepared by dissolving in tertiary distilled water. Each group was intraperitoneally injected with 0.5 mg of phenol red 10 mg / kg after 30 minutes of oral administration.
  • rats of each group were anesthetized using diethyl ether, and the abdominal aorta was cut and bleeded, and the entire trachea was excised.
  • the separated organs were placed in 1 ml of physiological saline, and the tissues were washed and vortexed (washing / vortexing) for 30 minutes, and then the washing solution was centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml was taken from the separated supernatant, and 0.1 ml of 1M NaOH was added to the solution to develop color. This solution was measured at absorbance 546 nm to determine the phenol red extracted from the airways. The amount of phenol red to airway was determined by dividing the weight by airway weight.
  • mice Male SD Rat mice were divided into 6 groups, respectively, into a normal group, a control group, and a sample group.
  • the normal group was orally administered 500 ⁇ L of saline
  • the positive control group was orally administered 1 ml of ammonium chloride (NH 4 Cl) at a concentration of 1000 mg / kg.
  • NH 4 Cl ammonium chloride
  • the experimental group was orally administered 500 ⁇ L at 50 mg / kg concentrations of hexane, chloroform, ethyl acetate, butanol, and water fractions of the yellow-chilled leaf hydrothermal extract, and the sample was prepared by dissolving in saline and ethanol. It melt
  • the content of phenol red was 2.6 ⁇ g / g in the normal group, and 3.9 ⁇ g / g in the NH 4 Cl group used as the positive control group.
  • the phenol red content of the chloroform fraction of the Hwangchil leaf hydrothermal extract was the highest. It was confirmed that the chloroform fraction of the Hwangchil leaf hydrothermal extract showed the highest activity (sputum activity) with sputum discharge ability by increasing the mucus secretion.
  • male SD Rat rats were divided into two groups, a control group and a sample group. After 1 hour of oral administration of Hwangchil water extract (200 mg / kg) to male rats, the rats were placed in the plestismograph chamber (Buxco, USA) to induce stability, and then coughed with a cough inducer (citric acid). Induced. Rats were exposed to 1 M citric acid for 10 minutes and the cough generated at this time was measured for 10 minutes.
  • a cough inducer citric acid
  • Table 1 The results of the antitussive activity of the hot-water extract of Hwangchil leaves are shown. Dose (mg / kg) Cough Count (times / min) Cough suppression rate (%) Control - 110.2 ⁇ 17.3 Experimental group (Hwangchil hot water extract) 200 88.0 ⁇ 4.7 20.1
  • the male nude mice were injected subcutaneously with 0.02 mL of 10, 50, 70, and 100 mg / mL concentrations in female nude mice, respectively. Fractions for each solvent were injected subcutaneously at 0.02 mL at 50 mg / mL concentration, respectively. After 30 minutes, the presence or absence of allergy of the fractions by solvent of the Hwangchil leaf hydrothermal extract and the Hwangchil leaf hydrothermal extract was determined.
  • Figure 6 shows the results of allergy in the skin sensitization test animal model of the solvent-specific fractions of the Hwangchil hydrothermal extract and Hwangchil hydrothermal extract. As shown in FIG. 6A, the results were confirmed by treating Hwangchil leaf hydrothermal extract by concentration (10, 50, 70, 100 mg / mL) and confirming that the allergic reaction was not induced at the 50 mg / mL concentration (final concentration 1 mg) or less. (Table 2).
  • allergic reactions to the skin will not be induced at 50 mg / mL concentration (final concentration of 1 mg), hexane, chloroform, and water fractions of 50 mg / mL (final concentration of 1 mg) of yellow lacquer leaf hydrothermal extract (final concentration 1 mg). It was confirmed that it is stable without toxicity. In particular, the chloroform fraction was confirmed to contain an active ingredient that is stable to antitussive or expectorant activity.
  • the antitussive or expectorant pharmaceutical composition of the present invention is easy to take as a health supplement containing the active ingredient because it is excellent in removing the antitoxicity and antitussive effect by using natural raw materials and without the antitussive or expectorant effect, even if taking long-term side effects It can be used safely without this.
  • Hwangchil leaf hot water extract as a composition for Jinhae or expectorant has no side effects to the human body and is excellent in removing antitussive or expectorant, so it is easy to take as a pharmaceutical composition or health supplement containing it as an active ingredient, and no side effects even after long-term use It can be used stably, and by replacing raw materials for removal of Jinhae or expectorant with plants that live in nature, it is expected to reduce the production cost and import substitution and export effect through industrialization.

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Abstract

Provided is a high value-added functional dietary supplement for cough relief or the discharge of phlegm, containing a hot water extract of Dendropanax morbiferus H.Lev. leaves, which are Korean natural resources, as an active ingredient. Provided is a composition for cough relief or the discharge of phlegm which is safe without side effects even if being taken for a long time since a natural material is used, and contains a crude extract of Dendropanax morbiferus H.Lev. leaves solubilized and extracted from any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixture solvent thereof, and an aprotic extract from any one of hexane, chloroform, dichloromethane and ethyl acetate as an aprotic solvent, as active ingredients.

Description

황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물Pharmaceutical composition for Jinhae or expectorant containing hot water extract of yellow lacquer leaves
본 발명은 천연물인 황칠 유래 추출물을 유효성분을 사용한 진해 또는 거담용 조성물에 관한 것으로서, 보다 구체적으로는 천연원료인 황칠 잎 열수추출물을 유효성분으로 이용하여 독성 및 부작용 없이 안전하게 사용될 수 있으며, 황칠 잎 열수추출물을 장기간 복용하여도 부작용 없이 기침해소(진해) 또는 가래제거(거담)가 가능한 황칠 추출물을 함유하는 진해 또는 거담용 약학 조성물에 관한 것이다. The present invention relates to a composition for antitussive or expectorant using natural extracts derived from hwangchil as an active ingredient, and more specifically, using hwangchil leaf hot water extract as a natural ingredient as an active ingredient and can be safely used without toxicity and side effects. The present invention relates to a pharmaceutical composition for antitussives or expectorants, which contains a yellow lacquer extract capable of relieving cough (jinhae) or sputum removal (sputum) without side effects even after taking hot water extract for a long time.
황칠나무는 생물학적 분류로 현화식물문, 쌍떡잎식물간, 산형화목, 두릅나무과에 속하는 식물로서, 우리나라 고유의 전통도료로 경제적 가치가 있으며, 완도, 해남 및 보길도 등 전라남도의 서남해안 지역과 제주도의 한라산 일대에 자생하는 상록활엽교목이다.Hwangchil-tree is a biological classification belonging to the flowering plants, dicotyledonous, mountain-shaped tree, arboraceae family, and has economic value as a traditional paint of Korea, and the southwest coast of Jeollanam-do and Halla-san of Jeju-do, including Wando, Haenam and Bogildo It is an evergreen broad-leaved arboreous tree native to the area.
상기 황칠나무는 높이가 15 m에 달하고, 어린 가지는 녹색이며 털이 없다. 상기 황칠나무의 잎은 어긋나고 달걀 모양 또는 타원형이며, 꽃은 6월에 연한 황록색으로 피고 양성화이다. 상기 황칠나무의 열매는 타원형으로, 10월에 흑색으로 열린다. 전통 공예인 황칠에 사용되는 상기 황칠나무의 진액은 8월 내지 9월에 채취한다.The hwangchil trees reach a height of 15 m, young branches are green and hairless. The leaves of the Hwangchil tree are shifted and egg-shaped or oval, and the flowers bloom in light yellow-green in June and are bisexual. The fruit of the Hwangchil-tree is oval, opened in black in October. The essence of the yellow lacquer tree, which is used for the traditional craft, Hwangchil, is collected in August to September.
겨울에도 낙엽이 지지 않는 수종으로 수피에 상처를 주면 황색의 수지액이 나오는데 이것을 황칠(yellow lacquer)이라고 한다. 황칠은 투명한 천연 도료로 삼국시대부터 가구, 가죽, 옷감, 금속, 종이 등에 사용되어 왔으며, 특히 황칠을 한 목공예품은 금빛을 띠면서도 바탕의 나무결을 생생하게 떠오르게 하여 목공예품을 한층 화사하게 하는 장점을 가지고 있어 목공예 분야에 널리 사용되어 왔다. It is a species that leaves no leaves even in winter. When the bark is wounded, yellow resin solution comes out, which is called yellow lacquer. Hwangchil has been used in furniture, leather, cloth, metal and paper since the Three Kingdoms since it was a transparent natural paint. Especially, the hwangchil woodwork has the advantage of making woodwork more bright by vividly bringing up the wood texture of the base. Widely used in the woodworking field.
황칠은 도료로서의 작용 이외에도 약리작용이 일부 알려져 있는데, 명나라 이시진이 저술한 본초강목에서 황칠은 번열을 제거하며, 술로 인한 황달과 눈 이 노랗게 되는 증상을 풀어주고, 나병치료에 효과가 있는 것으로 알려져 있다.In addition to acting as a paint, it is known to have some pharmacological effects.In the herbal wood, written by Ming Yi Sijin, it is known that it removes heat and relieves jaundice and yellowing caused by alcohol, and is effective in treating leprosy. .
황칠의 성분은 황금색 도막을 형성하는 도료성분인 비휘발성분 66.7%, 방향성분 10.8%, 수분 8.1%, 고형분 14.4%로 구성되어 있다. 방향성분은 주로 세스퀴테르펜류의 β-쿠베벤(cubebene), γ-셀리넨(selinene), δ-카디넨 (cadinene)으로 이루어져 있으며 이들 성분이 신경계에 대한 진정작용과 강장작용을 나타낸다고 알려져 있다. Hwangchil's components consist of 66.7% of non-volatile components, 10.8% of aromatic components, 8.1% of moisture, and 14.4% of solid components, which are the paint components that form the golden coating. The aromatic component consists mainly of sesquiterpenes, β-cubebene, γ-selinene, and δ-cadinene, which are known to exhibit sedative and tonic effects on the nervous system. .
또한, 황칠나무 수액을 바르게 되면 머리를 맑게 하고 심신을 편안하게 해주는 효과가 있음은 물론, 은은하고 매혹적인 향기를 품고 있어 여성들의 향수로도 사용되고 있으며, 예로부터 민간에 구전되어 오는 안식향(安息香, pterostyrax hispidum)을 제조하는 데 사용하였으며, 황칠 수액으로 안식향을 만들었던 만큼 황칠 제품에서 풍기는 향은 독특하다고 알려져 있다. Also, applying sapwood sap to clear the head and relax the mind and body, as well as a fascinating and fascinating fragrance, has been used as a perfume for women, and it has been used in the folklore since ancient times (pterostyrax hispidum) ), And the scent of Hwangchil products is known to be unique as it was made of Benzoin sap.
특히, 항산화, 항균 또는 항암 등의 효과를 갖는 것으로 알려져 황칠에서 특정성분을 분리할 경우 의약품으로 활용할 수 있을 뿐만 아니라 옻칠과 달리 피부 알레르기를 거의 유발하지 않고 취급이 용이하나 황칠을 적당히 2차 가공하여 건강식품으로도 널리 활용할 수 있음을 알 수 있다. In particular, it is known to have antioxidant, antibacterial, or anti-cancer effects, and it can be used as a medicine when separating certain ingredients from Hwangchil. It can be seen that it can be widely used as a health food.
황칠나무잎을 이용한 국내 선행문헌으로는 국내 공개특허공보 제10-2011-0091959호에는 해양생태환경을 파괴하여 생태교란동물로 지정된 강장동물인 해파리의 추출액만을 이용하여 유산균으로 발효시켜 얻어진 발효액을 주원료를 이용하여 정상적인 암세포를 사멸시키는 Apoptosis 유도작용을 이용한 암의 치료와 개선효과를 갖는 건강기능식품 조성물 및 그의 제조방법에 관하여 개시되어있고,In Korean Patent Publication No. 10-2011-0091959, which uses yellow chile leaves, the main ingredient is fermentation broth obtained by fermenting with lactic acid bacteria using only extract of jellyfish, a tonic animal designated as an ecological disturbance by destroying marine ecological environment. The present invention relates to a health functional food composition having a therapeutic effect and an improvement effect using apoptosis-inducing action of killing normal cancer cells, and a method of manufacturing the same,
국내 공개특허공보 제10-2011-00801387호에서 피부자극 및 피부염을 방지하여 모발 및 두피를 보호하는 효과를 제공하는 황칠나무 추출물을 포함하는 모발용 조성물 첨가제 및 그 제조방법이, 국내 공개특허공보 제10-2007-0098529호에는 살균 및 제독기능과 방부기능을 가지는 기능성 황칠을 제조하는 살균 및 제독기능을 가지는 기능성 황칠 제조방법에 관하여 개시되어있으며.In Korean Unexamined Patent Publication No. 10-2011-00801387, a composition additive for hair and a method for manufacturing the same, comprising a extract of Hilchi chinensis which provides an effect of protecting skin and scalp by preventing skin irritation and dermatitis, -2007-0098529 discloses a method for preparing functional yellow lacquer having a bactericidal and detoxifying function for producing a functional yellow lacquer having a bactericidal and detoxifying function and an antiseptic function.
국내 공개특허공보 특1998-026683호에는 황칠나무에서 수액을 채취하여 제조되는 황금색 천연도료인 황칠 도료용 용제 조성물에 관하여 개시되어 있지만, 이와 같은 선행기술에서는 본 발명에서와 같이 천연원료인 황칠 잎 열수 추출물을 유효성분으로 이용하여 독성 및 부작용 없이 안전하게 사용될 수 있으며, 황칠 잎 열수 추출물을 장기간 복용하여도 부작용 없이 기침해소(진해) 또는 가래제거(거담)가 가능하도록 하기 위한 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물과는 차이를 보인다.Korean Laid-Open Patent Publication No. 1998-026683 discloses a solvent composition for a yellow lacquer paint, which is a golden natural paint prepared by extracting sap from a yellow lacquer tree, but in the prior art, hot water of a yellow lacquer leaf as a natural raw material. By using the extract as an active ingredient, it can be safely used without toxicity and side effects, and it contains Hwangchil leaf hydrothermal extract to enable cough relief (jinhae) or sputum removal (sputum) without side effects even after long-term use of Hwangchil leaf hydrothermal extract. It differs from pharmaceutical compositions for antitussive or expectorant.
또한 황칠나무 잎을 이용한 산업화를 고려할 때 g당 9만원 내지 10만원에 판매되는 수액에 비해 kg당 12,000원 내지 25,000원 정도 판매되는 황칠나무 잎(생잎 기준)을 사용했을 때 훨씬 더 경제성 더 뛰어남을 알 수 있다.In addition, considering the industrialization using the yellow lacquer leaves, it is much more economical to use the yellow lacquer leaves (based on fresh leaves), which sell for 12,000 to 25,000 won per kg, compared to the sap sold for 90,000 to 100,000 won per g. Able to know.
그러나, 황칠 원액의 주성분인 세스퀴테르펜의 일부 물질은 독특한 방향특성을 나타낼 뿐만 아니라 쓴맛을 나타내게 되는데, 이 방향성과 쓴맛의 특성으로 인하여 황칠 원액을 건강식품화 할 경우 오히려 소비자에게 거부감을 줄 수 있고, 진해 또는 거담에 관련한 연구는 전혀 알려져 있지 않다. However, some substances of sesquiterpene, the main component of the Hwangchil liquor, not only show unique fragrance characteristics but also have a bitter taste, and this aroma and bitterness characteristics can cause consumer rejection when health foods are prepared. There is no known research related to nausea or Jinhae.
이와 같은 문제를 해결하기 위해서 본 발명은 황칠 수액에 비해 경제성이 뛰어난 천연원료인 황칠 잎 열수 추출물을 유효성분으로 이용하여 독성 및 부작용 없이 안전하게 사용될 수 있으며, 황칠 잎 열수 추출물을 장기간 복용하여도 부작용 없이 안전한 숙취를 할 수 있도록 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물을 제공하고자한다.In order to solve such a problem, the present invention can be safely used without toxic and side effects by using Hwangchil leaf hydrothermal extract, which is an economical natural material, as an active ingredient, which is superior to Hwangchil sap. To provide a pharmaceutical composition for antitussive or expectorant containing Hwangchil leaf hydrothermal extract to enable a safe hangover.
본 발명은 진해 또는 거담활성에 유효하게 작용하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 조성물에 관한 것으로, 우리나라 천연자원인 황칠 잎 열수 추출물을 유효성분으로 하는 진해 또는 거담해소에 작용하는 약학조성물 및 고부가가치를 갖는 기능성 건강식품을 제조할 수 있으며, 장기간 복용하여도 부작용 없이 안전한 진해 또는 거담용 조성물을 제공하고자 한다. The present invention relates to a composition for antitussive or expectorant containing Hwangchil leaf hydrothermal extract that effectively acts on antitussive or expectorant activity. And it is possible to manufacture a functional health food having a high value, and to provide a safe antitussive or expectorant composition without side effects even when taken for a long time.
상기 과제를 해결하기 위해 본 발명은 황칠 잎 추출물 또는 비극성가용추출물을 유효성분으로 포함하는 진해 또는 거담용 약학 조성물을 제공한다.In order to solve the above problems, the present invention provides a pharmaceutical composition for antitussive or expectorant, comprising hwangchil leaf extract or non-polar soluble extract as an active ingredient.
황칠 잎 조추출물은 물, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올 또는 이들의 혼합용매 중 어느 하나에서 가용한 추출물 일 수 있으며, 상기 추출용매를 사용하여 추출한 황칠 잎 조추출물에 비극성용매로서 헥산, 클로로포름, 디클로메탄 및 에틸아세테이트 중 어느 하나를 분획용매로 사용하여 분획할 수 있다. The crude yellow leaf extract may be an extract available in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof, and hexane and chloroform as non-polar solvents in the crude yellow leaf extract extracted using the extracting solvent. , Dichloromethane and ethyl acetate can be fractionated using any one of the fractions.
상기 물, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올 또는 이들의 혼합용매 중 어느 하나에서 가용한 추출물을 유효성분으로 포함하는 것을 특징으로 하는 진해 또는 거담용 약학 조성물의 0.01 내지 99.9 중량%의 양으로 포함될 수 있으며, 상기 추출물의 1일당 투여량은 체중 kg당 10 내지 2000mg/kg의 양으로 음용할 수 있다. It is included in the amount of 0.01 to 99.9% by weight of the pharmaceutical composition for antitussive or expectorant, comprising an extract available in any one of the water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof as an active ingredient. The daily dosage of the extract may be consumed in an amount of 10 to 2000 mg / kg per kg body weight.
또한, 황칠 잎 조추출물에 비극성용매로서 헥산, 클로로포름, 디클로메탄 및 에틸아세테이트 중 어느 하나를 분획용매로 사용한 분획물을 유효성분으로 포함하는 것을 특징으로 하는 진해 또는 거담용 약학 조성물의 0.01 내지 99.9 중량%의 양으로 포함될 수 있으며, 상기 추출물의 1일당 투여량은 분획물이 체중 kg당 1 내지 1000mg/kg의 양으로 투여할 수 있다. In addition, 0.01 to 99.9 weight of the pharmaceutical composition for antitussive or expectorant, characterized in that the crude extract, which comprises a fraction using any one of hexane, chloroform, dichloromethane and ethyl acetate as a fraction solvent as a non-polar solvent as an active ingredient. It may be included in an amount of%, the daily dosage of the extract may be administered in an amount of 1 to 1000 mg / kg fraction per kg body weight.
더욱 바람직하게는 분획물로서 황칠 잎 열수 추출물의 클로로포름으로 분획한 분획물을 유효성분으로 포함하며, 상기 분획물은 전체 조성물의 0.01 내지 99.9중량%로 포함하며, 상기 진해 또는 거담용 약학 조성물의 1일당 투여량은 상기 분획물이 1 내지 1000 mg/kg 체중 포함되는 함량이다.More preferably, the fraction is fractionated with chloroform of the hydrothermal extract of Hwangchil leaves as an active ingredient, the fraction comprises 0.01 to 99.9% by weight of the total composition, the daily dose of the pharmaceutical composition for antitussive or expectorant Is the amount of the fraction contained 1 to 1000 mg / kg body weight.
또한, 상기 조성물은 호흡기 질환의 예방 또는 치료용 약학 조성물에 사용되며, 상기 호흡기 질환은 폐기종, 만성기관지염, 기관지선종, 고립성폐결절, 폐결핵, 농흉, 폐농양, 감기, 독감 또는 폐의 조직구 증식증인 호흡기 질환의 예방 또는 치료용 약학 조성물에 사용된다. 상기 조성물은 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸, 경피제, 좌제 또는 멸균 주사용 액으로 제형화된 호흡기 질환의 예방 또는 치료용 약학 조성물로 사용되는 것을 특징으로 한다.In addition, the composition is used in the pharmaceutical composition for the prevention or treatment of respiratory diseases, the respiratory diseases are emphysema, chronic bronchitis, bronchial adenomas, isolated pulmonary nodules, pulmonary tuberculosis, empyema, lung abscess, cold, flu or histiocytosis of the lung It is used in pharmaceutical compositions for the prevention or treatment of respiratory diseases. The composition is characterized in that it is used as a pharmaceutical composition for the prevention or treatment of respiratory diseases formulated in powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, transdermals, suppositories or sterile injectable solutions.
본 발명은 천연물인 황칠 잎 열수 추출물을 유효성분으로 사용함으로써 장기간 복용하여도 부작용 없이 기침해소(진해) 또는 가래제거(거담)를 할 수 있으며, 천연물인 황칠 잎 열수 추출물의 클로로포름 분획물을 유효성분으로 사용함으로써 장기간 복용하여도 부작용 없이 기침해소(진해) 또는 가래제거(거담)를 할 수 있는 현저한 효과가 있다. The present invention can be used to remove cough (jinhae) or sputum (sputum) without side effects even after long-term use by using a natural Hwangchil leaf hydrothermal extract as an active ingredient, chloroform fraction of the natural Hwangchil leaf hydrothermal extract as an active ingredient By using it for a long time, there is a remarkable effect of eliminating cough (jinhae) or sputum removal (sputum) without side effects.
도 1은 황칠 잎을 나타낸 사진이다.1 is a photograph showing the yellow chil leaves.
도 2은 황칠 잎 열수 추출물의 추출과 분획 모식도를 나타낸다.Figure 2 shows the extraction and fractional schematic of the Hwangchil leaf hydrothermal extract.
도 3는 황칠 잎 열수 추출물에 대한 기도에서의 점액분비량 측정결과를 나타낸다.Figure 3 shows the results of the measurement of the mucus secretion in the airway for the hot-water extract of yellow chile.
도 4은 황칠 잎 열수 추출물 및 메탄올 추출물에 대한 기도에서의 점액분비량 측정결과를 나타낸다.Figure 4 shows the results of the measurement of mucus secretion in the airway for the hot water extract of the yellow lacquer extract and methanol extract.
도 5는 황칠 잎 열수 추출물의 용매별 분획물에 대한 기도에서의 점액분비량 측정결과를 나타낸다.Figure 5 shows the results of the measurement of the mucus secretion in the airway for the solvent-specific fractions of the yellow-leaf leaf hydrothermal extract.
도 6은 황칠 잎 열수 추출물 및 황칠 열수 추출물의 용매별 분획물의 피부감작실험 동물모델에서의 allergy 결과를 나타낸다.Figure 6 shows the results of allergy in the skin sensitization test animal model of the solvent-specific fractions of the Hwangchil hydrothermal extract and Hwangchil hydrothermal extract.
본 발명은 황칠 잎 조추출물 또는 비극성가용추출물을 유효성분으로 포함하는 진해 또는 거담용 약학 조성물을 제공한다.The present invention provides a pharmaceutical composition for antitussive or expectoration comprising crude yellow leaf extract or non-polar soluble extract as an active ingredient.
황칠 잎 조추출물은 물, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올 또는 이들의 혼합용매 중 어느 하나에서 가용한 추출물 일 수 있으며, 상기 추출용매를 사용하여 추출한 황칠 잎 조추출물에 비극성용매로서 헥산, 클로로포름, 디클로메탄 및 에틸아세테이트 중 어느 하나를 분획용매로 사용하여 분획할 수 있다.The crude yellow leaf extract may be an extract available in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof, and hexane and chloroform as non-polar solvents in the crude yellow leaf extract extracted using the extracting solvent. , Dichloromethane and ethyl acetate can be fractionated using any one of the fractions.
또한, 상기 추출물은 전체 조성물의 0.01 내지 99.9 중량%의 양으로 포함되어 진해 또는 거담용 음료로서 제공될 수 있고, 상기 추출물의 1일당 투여량은 체중 kg당 10 내지 2000mg/kg의 양으로 음용할 수 있다. In addition, the extract may be included as an amount of 0.01 to 99.9% by weight of the total composition to serve as an antitussive or expectorant beverage, the daily dosage of the extract is to be drinking in an amount of 10 to 2000mg / kg per kg body weight Can be.
황칠 잎 열수 추출물의 클로로포름으로 분획한 분획물을 유효성분으로 포함하며, 상기 분획물은 전체 조성물의 0.01 내지 99.9중량%로 포함하며, 상기 진해 또는 거담용 약학 조성물의 1일당 투여량은 상기 분획물이 1 내지 1000 mg/kg 체중 포함되는 함량이다.It comprises a fraction fractionated with chloroform of the Hwangchil leaf hydrothermal extract as an active ingredient, the fraction comprises 0.01 to 99.9% by weight of the total composition, the daily dose of the antitussive or expectorant pharmaceutical composition is 1 to the fraction 1000 mg / kg body weight content.
진해 또는 거담용 조성물의 추출방법은 황칠 잎을 증류수로 수세한 다음 증류수와 혼합하여 열수 추출하는 단계; 상기 추출물을 여과한 농축하여 동결건조하는 단계; 상기 단계의 추출물을 분획용매에 용해시켜 분획물을 얻는 감압여과 장치로 여과, 농축하여 동결건조하는 단계를 포함하는 추출방법이 제공된다.Extraction method of the composition for Jinhae or expectorant is a step of washing the hot-chil leaves with distilled water and then mixed with distilled water to extract hot water; Lyophilizing the filtered and concentrated extract; An extraction method is provided, including filtration and concentration of the extract of the above step by dissolving in a fractional solvent to obtain a fraction by filtration, concentration, and lyophilization.
또한, 상기 추출물은 호흡기 질환의 예방 또는 치료용 약학 조성물에 사용되며, 상기 조성물은 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸, 경피제, 좌제 또는 멸균 주사용 액으로 제형화된 것인 호흡기 질환의 예방 또는 치료용 약학 조성물에 사용되는 것을 특징으로 한다.The extracts are also used in pharmaceutical compositions for the prevention or treatment of respiratory diseases, which are formulated as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, transdermals, suppositories or sterile injectable solutions. It is characterized in that it is used in the pharmaceutical composition for the prevention or treatment of respiratory diseases.
이하 첨부된 도면을 참조하여 본 발명의 실시예를 상세히 설명한다. 하기에서 본 발명을 설명함에 있어서, 공지기능 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략하였다.Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, when it is determined that the detailed description of the known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof is omitted.
1. 황칠 잎 열수 추출물 및 분획물 제조1. Preparation of Hwangchil Leaf Hot Water Extract and Fraction
도 2은 황칠 잎 열수 추출물 및 유기용매에 의한 분획물을 얻는 과정을 나타낸다. 건조된 황칠 1kg을 증류수로 수세한 다음 증류수 20L를 가하고, 전기약탕기로 100℃에서 3시간 동안 가열, 추출하였다. Figure 2 shows the process of obtaining a fraction by the yellow-leaf leaf hydrothermal extract and the organic solvent. 1 kg of dried yellow lacquer was washed with distilled water, 20 L of distilled water was added thereto, and heated and extracted at 100 ° C. for 3 hours using an electric bath.
추출된 용액은 400 메쉬 여과포로 여과한 다음 감압회전농축기로 농축하였다. 여과 후 남은 잔사에 다시 동량의 증류수를 사용하여 동일 과정으로 2번 더 추출, 여과 및 감압 농축한다. 농축된 열수 추출물을 동결건조기 (Freeze dryer)에서 동결건조 하였다. 건조 후 황칠 잎 열수 추출물 279g (24.9%)을 얻었다. The extracted solution was filtered through a 400 mesh filter cloth and then concentrated using a vacuum concentrator. After filtration, the residue was extracted, filtered and concentrated under reduced pressure two more times using the same amount of distilled water. The concentrated hot water extract was lyophilized in a freeze dryer. After drying, 279 g (24.9%) of yellow-hot leaf hydrothermal extract was obtained.
2. 황칠 잎의 극성용매, 비극성용매 가용 분획물의 제조2. Preparation of Polar Solvent and Nonpolar Solvent Soluble Fraction
도 2에 도시된 바와 같이 제조된 황칠 잎 열수 추출물을 유기 용매를 이용하여 분획물을 제조하였다. 황칠 잎의 극성용매, 비극성용매 가용 분획물의 제조는 황칠 잎 열수 추출물 249g을 증류수 1L에 완전히 용해시킨 후 분획 여두 깔대기에 넣고 헥산(Hexane) 1L를 첨가하여 water층과 hexane 층을 분리하였고 이와 같은 공정을 3번 반복하였다. Fractions were prepared using the organic solvent of the Hwangchil leaf hydrothermal extract prepared as shown in FIG. 2. For the preparation of the polar solvent and the non-polar solvent soluble fraction of the yellow lacquer leaves, 249 g of the hot water extracts of the yellow lacquer leaf were completely dissolved in 1 L of distilled water, and then placed in a separatory funnel and 1 L of hexane was added to separate the water and hexane layers. Was repeated three times.
동일한 과정을 통해 클로로포롬(chloroform), 에틸아세테이트(ethyl acetate), 부탄올(butanol), 물을 순차적으로 가하여 각 분획물을 얻었고, 얻어진 각각의 분획물을 감압여과 장치로 여과하여 농축한 후 동결 건조하여 용매를 완전히 제거한 뒤 본 실험에 사용하였다. Through the same process, chloroform, ethyl acetate, butanol, and water were added sequentially to obtain each fraction. Each of the obtained fractions was filtered through a vacuum filter, concentrated, and freeze-dried. After completely removed was used in this experiment.
2.1. 헥산 가용성 분획 분리2.1. Hexane Soluble Fraction Separation
황칠 잎 열수 추출물 249g을 lL의 증류수에 완전히 용해시킨 후에 분획여두에 넣고 헥산 1L를 첨가하여 헥산 불용성층(수층)과 헥산가용성층을 분리하였다. 다시 헥산 불용성층(수층)을 대상으로 동일한 공정을 3번 반복하여 헥산 불용성 분획 및 가용성 분획을 수집하였다.After dissolving 249 g of Hwangchil leaf hot water extract in 1 liter of distilled water, the mixture was put into a fractional filter and 1 L of hexane was added to separate the hexane-insoluble layer (aqueous layer) and the hexane-soluble layer. Again, the same process was repeated three times for the hexane insoluble layer (aqueous layer) to collect the hexane insoluble fraction and the soluble fraction.
2.2. 클로로포름 가용성 분획분리2.2. Chloroform Soluble Fractionation
헥산불용성 분획(수층)에 클로로포름 5L를 가하여 섞은 후에 클로로포름가용성 분획 및 불용성 분획을 분리하였고, 클로로포름 불용성층(수층)을 대상으로 동일한 공정을 3번 반복하여 클로로포름 불용성 분획 및 가용성 분획을 수집하였다.After adding 5 L of chloroform to the hexane insoluble fraction (aqueous layer), the chloroform soluble fraction and the insoluble fraction were separated, and the same procedure was repeated three times for the chloroform insoluble layer (aqueous layer) to collect the chloroform insoluble fraction and the soluble fraction.
2.3. 에틸아세테이트 가용성 분획분리2.3. Ethyl acetate soluble fractionation
클로로포름 불용성 분획(수층)에 에틸아세테이트 5L를 가하여 섞은 후에 에틸아세테이트 가용성 분획 및 불용성 분획을 분리하였고, 에틸아세테이트 불용성층(수층)을 대상으로 동일한 공정을 3번 반복하여 에틸아세테이트 불용성 분획 및 가용성 분획를 수집하였다.After adding 5 L of ethyl acetate to the chloroform insoluble fraction (aqueous layer), the ethyl acetate soluble fraction and the insoluble fraction were separated, and the same process was repeated three times on the ethyl acetate insoluble layer (aqueous layer) to collect the ethyl acetate insoluble fraction and the soluble fraction. It was.
2.4. 부탄올 가용성 분획분리2.4. Butanol Soluble Fractionation
에틸아세테이트 불용선 분획(수층)에 부탄올 5L를 가하여 섞은 후에 부탄올 가용성 분획 및 불용성 분획을 분리하였고, 부탄올 불용성층을 대상으로 동일한 공정을 3번 반복하여 부탄올 불용성 분획 및 가용성 분획을 수집하였다.Butanol soluble fraction and insoluble fraction were separated by adding 5 L of butanol to the ethyl acetate insoluble fraction (aqueous layer), and the same process was repeated three times for the butanol insoluble layer to collect butanol insoluble fraction and soluble fraction.
2.5. 황칠 잎 열수 추출물 및 분획물 수득2.5. Obtained Hwangchil Leaf Hot Water Extract and Fraction
황칠 잎 열수 추출물 249g에서 헥산 가용성 분획, 클로로포름 가용성 분획, 에틸아세테이트 가용성 분획 및 부탄올 가용성 분획을 감압 농축한 후에 동결 건조하여 헥산분획 0.096g (0.04%), 클로로포름 분획 0.57g (0.84%), 에틸아세테이트 분획 4.04g (1.63%), 부탄올 분획 54.74g (10.28%) 및 물 분획 168.64g (67.84%)을 얻어 시료로 사용하였다The hexane soluble fraction, the chloroform soluble fraction, the ethyl acetate soluble fraction, and the butanol soluble fraction were concentrated under reduced pressure in 249 g of Hwangchil leaf hydrothermal extract, and then freeze-dried to obtain 0.096 g (0.04%) of hexane fraction, 0.57 g (0.84%) of chloroform fraction, and ethyl acetate. 4.04 g (1.63%) fraction, 54.74 g (10.28%) butanol fraction and 168.64 g (67.84%) water fraction were used as samples.
3. 실험 동물사육 3. Experimental Animal Breeding
황칠 추출물의 진해 또는 거담 효과 측정을 위한 실험동물로서 생후 5주령 된 수컷 ICR 마우스 (18-22g) 및 8주령 된 체중 320±100g 내외의 수컷 SD Rat 흰쥐를 (주)샘타코 (SAMTACO, Korea)부터 구입 하여 동물 사육실에서 일정한 조건(온도: 22±2℃, 습도:50±5%, 명암: 12시간 light/dark cycle)으로 일주일간 적응시킨 후 사용하였다.As a laboratory animal for measuring the antitussive or expectorant effect of the Hwangchil extract, male ICR mice (18-22g) aged 8-years old and male SD Rat rats weighing 320 ± 100g at 8 weeks old were sampled from SAMTACO, Korea. The product was used for one week in animal breeding room under constant conditions (temperature: 22 ± 2 ℃, humidity: 50 ± 5%, contrast: 12 hours light / dark cycle).
3.1. 페놀레드(phenol red)를 이용한 기도에서의 점액분비량 측정 (황칠 잎 열수 추출물)3.1. Determination of mucus secretion in the airway using phenol red (Hwangchil leaf hydrothermal extract)
황칠 잎 열수 추출물의 거담활성을 측정하기 위하여, 수컷 ICR 마우스를 정상군(normal), 양성대조군(control), 시료 투여군(sample)으로 각각 3군으로 나누었다. 정상군은 살린(saline) 100μL를 경구 투여하였고, 양성대조군은 암모니움클로라이드(NH4Cl)를 1000mg/kg 농도로 200μL를 경구 투여하였다. Male ICR mice were divided into three groups: normal, positive control, and sample administration groups. The normal group was orally administered 100 μL of saline, and the positive control group was orally administered 200 μL of ammonium chloride (NH 4 Cl) at a concentration of 1000 mg / kg.
실험군은 황칠 잎 열수 추출물을 각각 50, 100, 200mg/kg 농도로 100μL를 경구 투여하였으며, 시료는 saline에 녹여 조제하였고, NH4Cl은 3차 증류수에 녹여서 조제하였다. 각 군을 경구투여 1시간 경과 후 0.2㎖의 페놀레드(phenol red) 10mg/kg을 복강 주사하였다.The experimental group was orally administered 100 μL at 50, 100 and 200 mg / kg concentration of the Hwangchil leaf hot water extract, and the sample was dissolved in saline, and NH4Cl was dissolved in tertiary distilled water. Each group was intraperitoneally injected with 0.2 ml of phenol red 10 mg / kg after 1 hour of oral administration.
각 군의 생쥐는 30분 경과 후 디에틸이더(dimethylether)를 이용하여 마취시키고 복부 대동맥을 절단하여 방혈 시킨 후 기관(trachea) 전체를 절제하였다. 분리된 기관을 1㎖의 생리식염수에 넣어 30분 간 조직을 세척 및 볼텍싱 (washing/ vortexing) 한 후 그 세정액을 원심분리(10,000rpm, 5min, 실온)하였다. 분리한 상층액으로 부터 0.9㎖를 취하여 이 용액에 0.1㎖의 1M NaOH를 가하여 발색시켰다. 이 용액을 흡광도 546nm에서 측정하여 기도로부터 추출된 페놀레드를 측정하였다. 이를 적출한 기도의 무게로 나누어 기도에 대한 페놀레드의 비율을 구하여 기도에서의 점액분비량을 구하였다. Mice in each group were anesthetized using diethyl ether (dimethylether) after 30 minutes, and the abdominal aorta was cut and bleeded, and then the entire trachea was excised. The separated organs were placed in 1 ml of saline, and the tissues were washed and vortexed for 30 minutes, and then the washing solution was centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml was taken from the separated supernatant, and 0.1 ml of 1M NaOH was added to the solution to develop color. This solution was measured at absorbance 546 nm to determine the phenol red extracted from the airways. The amount of phenol red to airway was determined by dividing the weight by airway weight.
페놀레드를 이용한 기도에서의 점액분비량 측정결과, 도 3에 나타낸 바와 같이 기도 1g당 페놀레드 함량은 정상군에서 2.436μg/g, 양성대조군으로 사용한 NH4Cl군에서 3.476μg/g, 실험군인 황칠 잎 열수 추출물을 50, 100, 200mg/kg 투여한 군에서는 각각 2.958 (21.4%), 3.108 (27.6%), 3.105 μg/g (27%) 값을 얻었다. 이는 황칠 잎 열수 추출물이 농도 의존적으로 페놀레드 함량 증가 즉, 점액분비량을 증가시킴으로서 객담 배출능이 우수한 활성(거담활성)을 나타냄을 확인 하였다.As a result of measuring mucus secretion in the airway using phenol red, as shown in FIG. 3, the content of phenol red per 1 g of airway was 2.436 μg / g in the normal group, 3.476 μg / g in the NH4Cl group used as the positive control group, and the experimental group of Hwangchil leaves 2.958 (21.4%), 3.108 (27.6%), and 3.105 μg / g (27%) were obtained in the 50, 100 and 200 mg / kg groups. It was confirmed that the extract of Hwangchil leaves exhibited excellent activity (sputum activity) by increasing phenol red content, that is, increasing mucus secretion, in a concentration-dependent manner.
3.2. 페놀레드(phenol red)를 이용한 기도에서의 점액분비량 측정 (황칠 잎 열수 추출물과 메탄올 추출물 비교)3.2. Determination of Mucus Secretion in the Airway Using Phenol Red (Compared with Hwangchil Leaf Hydrothermal Extract and Methanol Extract)
황칠 잎 열수 추출물의 진해활성을 측정하기 위하여, 수컷 SD Rat 생쥐를 정상군(normal), 정상대조군(control), 시료 투여군(sample)으로 각각 3군으로 나누었다. 정상군은 살린(saline) 500μL를 경구 투여하였고, 양성대조군은 암모니움클로라이드(NH4Cl)을 1000mg/kg 농도로 1ml을 경구 투여하였다. Male SD Rat mice were divided into three groups: normal, control, and sample administration. The normal group was orally administered 500 μL of saline, and the positive control group was orally administered 1 ml of ammonium chloride (NH 4 Cl) at a concentration of 1000 mg / kg.
실험군은 황칠 잎 열수 추출물, 황칠 잎 메탄올 추출물을 각각 200mg/kg농도로 500μL를 경구 투여하였으며, 시료는 saline에 녹여 조제하였고, 양성대조군에서 사용된 NH4Cl은 3차 증류수에 녹여서 조제하였다. 각 군을 경구투여 30분 경과 후 0.5㎖의 페놀레드(phenol red) 10mg/kg을 복강 주사하였다. The experimental group was orally administered Hwangchil leaf hydrothermal extract and 500ml L of hwangchil leaf methanol extract at a concentration of 200mg / kg, respectively, the sample was prepared by dissolving in saline, NH4Cl used in the positive control was prepared by dissolving in tertiary distilled water. Each group was intraperitoneally injected with 0.5 mg of phenol red 10 mg / kg after 30 minutes of oral administration.
각 군의 흰쥐를 30분 경과 후 디에틸이더(dimethylether)를 이용하여 마취시키고 복부 대동맥을 절단하여 방혈 시킨 후 기관(trachea) 전체를 절제하였다. 분리된 기관을 1㎖의 생리식염수에 넣어 30분간 조직을 세척 및 볼텍싱(washing/vortexing) 한 후 그 세정액을 원심분리(10,000rpm, 5min, 실온)하였다. 분리한 상층액으로 부터 0.9㎖를 취하여 이 용액에 0.1㎖의 1M NaOH를 가하여 발색시켰다. 이 용액을 흡광도 546nm에서 측정하여 기도로부터 추출된 페놀레드를 측정하였다. 이를 적출한 기도의 무게로 나누어 기도에 대한 페놀레드의 비율을 구하여 기도에서의 점액분비량을 구하였다. After 30 minutes, rats of each group were anesthetized using diethyl ether, and the abdominal aorta was cut and bleeded, and the entire trachea was excised. The separated organs were placed in 1 ml of physiological saline, and the tissues were washed and vortexed (washing / vortexing) for 30 minutes, and then the washing solution was centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml was taken from the separated supernatant, and 0.1 ml of 1M NaOH was added to the solution to develop color. This solution was measured at absorbance 546 nm to determine the phenol red extracted from the airways. The amount of phenol red to airway was determined by dividing the weight by airway weight.
페놀레드를 이용한 기도에서의 점액분비량 측정결과, 도 4에 나타낸 바와 같이 페놀레드 함량은 정상군에서는 2.6μg/g, 양성대조군으로 사용한 NH4Cl군에서 3.9μg/g, 실험군인 황칠 잎 열수 추출물 (200mg/kg), 황칠 잎 메탄올 추출물 (200mg/kg) 투여군에서 각각 3.1μg/g (19.2%), 2.2μg/g (효과 없음) 값을 얻었다. 이는 황칠 잎 열수 추출물이 메탄올 추출물 보다 페놀레드 함량 증가 즉, 점액분비량을 증가시킴으로서 객담배출능이 우수한 활성(거담활성)을 나타냄을 재확인 하였다.As a result of measuring mucus secretion in the airway using phenol red, as shown in FIG. 4, the content of phenol red was 2.6 μg / g in the normal group, 3.9 μg / g in the NH4Cl group used as the positive control group, and Hwangchil leaf hydrothermal extract (200 mg) as an experimental group. / kg), yellow mussel leaf methanol extract (200mg / kg) administration group obtained 3.1μg / g (19.2%), 2.2μg / g (no effect) value, respectively. It was reconfirmed that the Hwangchil leaf hydrothermal extract exhibited an excellent sputum releasing activity by increasing the phenol red content, that is, the amount of mucus secretion, than the methanol extract.
3.3. 페놀레드(phenol red)를 이용한 기도에서의 점액분비량 측정 (황칠 잎 열수 추출물의 용매별 분획물 비교)3.3. Determination of Mucus Secretion in the Airway Using Phenol Red (Comparison of Solvent Fractions of Hot Water Extracts from Yellow Chili Leaves)
황칠 잎 열수 추출물의 진해활성을 측정하기 위하여, 수컷 SD Rat 생쥐를 정상군(normal), 정상대조군(control), 시료 투여군(sample)으로 각각 6군으로 나누었다. 정상군은 살린(saline) 500μL를 경구 투여하였고, 양성대조군은 암모니움클로라이드(NH4Cl)을 1000 mg/kg 농도로 1ml를 경구 투여하였다. In order to measure the antitussive activity of Hwangchil leaf hot water extract, male SD Rat mice were divided into 6 groups, respectively, into a normal group, a control group, and a sample group. The normal group was orally administered 500 μL of saline, and the positive control group was orally administered 1 ml of ammonium chloride (NH 4 Cl) at a concentration of 1000 mg / kg.
실험군은 황칠 잎 열수 추출물 헥산, 클로로포름, 에틸아세테이트, 부탄올 및 물 분획물을 각각 50mg/kg 농도로 500μL를 경구 투여하였으며, 시료는 살린 및 에탄올에 녹여 조제하였고, 양성대조군에서 사용된 NH4Cl은 3차 증류수에 녹여서 조제하였다. 각 군을 경구투여 1시간 경과 후 0.5㎖의 페놀레드(phenol red) 10mg/kg을 복강 주사하였다. The experimental group was orally administered 500 μL at 50 mg / kg concentrations of hexane, chloroform, ethyl acetate, butanol, and water fractions of the yellow-chilled leaf hydrothermal extract, and the sample was prepared by dissolving in saline and ethanol. It melt | dissolved in and prepared. After 1 hour of oral administration, each group was intraperitoneally injected with 0.5 ml of phenol red 10 mg / kg.
각 군의 흰쥐를 30분 경과 후 디에틸이더(dimethylether)를 이용하여 마취시키고 복부 대동맥을 절단하여 방혈 시킨 후 기관 (trachea) 전체를 절제하였다. 분리된 기관을 1㎖의 생리식염수에 넣어 30분간 조직을 세척 및 볼텍싱 (washing/ vortexing)한 후 그 세정액을 원심분리(10,000rpm, 5min, 실온)하였다. 분리한 상층액으로 부터 0.9㎖를 취하여 이 용액에 0.1㎖의 1M NaOH를 가하여 발색시켰다. 이 용액을 흡광도 546nm에서 측정하여 기도로부터 추출된 페놀레드를 측정하였다. 이를 적출한 기도의 무게로 나누어 기도에 대한 페놀레드의 비율을 구하여 기도에서의 점액분비량을 구하였다. After 30 minutes, rats of each group were anesthetized using diethyl ether, and the abdominal aorta was cut and bleeded. The entire trachea was excised. The separated organs were placed in 1 ml of physiological saline, and the tissues were washed and vortexed for 30 minutes, followed by centrifugation (10,000 rpm, 5 min, room temperature). 0.9 ml was taken from the separated supernatant, and 0.1 ml of 1M NaOH was added to the solution to develop color. This solution was measured at absorbance 546 nm to determine the phenol red extracted from the airways. The amount of phenol red to airway was determined by dividing the weight by airway weight.
페놀레드(phenol red)를 이용한 기도에서의 점액분비량 측정결과, 도 5에 나타낸 바와 같이 페놀레드 함량은 정상군에서는 2.6μg/g, 양성대조군으로 사용한 NH4Cl군에서는 3.9μg/g, 실험군인 황칠 잎 열수 추출물의 클로로포름 분획물 (50 mg/kg), 황칠 잎 열수 추출물의 에틸아세테이트 분획물 (50mg/kg), 황칠 잎 열수추출물의 부탄올 분획물 (50mg/kg), 황칠 잎 열수 추출물의 물층 분획물 (50mg/kg) 투여군에서는 각각 4.8μg/g (84.6%), 3.8μg/g (46.2%), 2.5μg/g (유의성 없음), 2.6μg/g (유의성 없음) 값을 얻었다. As a result of measuring mucus secretion in the airway using phenol red, as shown in FIG. 5, the content of phenol red was 2.6 μg / g in the normal group, and 3.9 μg / g in the NH 4 Cl group used as the positive control group. Chloroform fraction (50 mg / kg) from the hot yellow leaf extract, ethyl acetate fraction (50 mg / kg) from the hot water extract, butanol fraction (50 mg / kg) from the hot water extract, water layer fraction from the hot water extract (50 mg) / kg), 4.8μg / g (84.6%), 3.8μg / g (46.2%), 2.5μg / g (unsignificant), 2.6μg / g (unsignificant) value obtained respectively.
이 중에서 황칠 잎 열수 추출물의 클로로포름 분획물의 페놀레드 함량 증가가 가장 높게 나타났다. 이는 황칠 잎 열수 추출물의 클로로포름 분획물이 점액분비량을 증가시킴으로서 객담배출능이 가장 우수한 활성(거담활성)을 나타냄을 확인 하였다.Among them, the phenol red content of the chloroform fraction of the Hwangchil leaf hydrothermal extract was the highest. It was confirmed that the chloroform fraction of the Hwangchil leaf hydrothermal extract showed the highest activity (sputum activity) with sputum discharge ability by increasing the mucus secretion.
3.4 시트르산을 이용한 황칠 잎 열수 추출물의 진해활성 측정3.4 Measurement of Antitussive Activity of Hot Water Extracts from Yellow Chil Leaves Using Citric Acid
황칠 잎 열수 추출물의 진해활성을 측정하기위하여, 수컷 SD Rat 흰쥐를 대조군(control), 시료 투여군(sample)으로 각각 2군으로 나누었다. 수컷 Rat 흰쥐에 황칠 물 추출물 (200 mg/kg)을 경구투여하고 1시간 후 흰쥐를 플레테시스모그래프 챔버 (Buxco, U.S.A)에 넣어 안정을 유도한 후, 기침 유발제 (시트르산)을 분무하여 기침을 유발하였다. 1 M의 시트르산에 흰쥐를 10분간 노출시키고 이때 발생하는 기침수를 10분 동안 측정하였다. In order to measure the antitussive activity of the hot-water extract of Hwangchil leaves, male SD Rat rats were divided into two groups, a control group and a sample group. After 1 hour of oral administration of Hwangchil water extract (200 mg / kg) to male rats, the rats were placed in the plestismograph chamber (Buxco, USA) to induce stability, and then coughed with a cough inducer (citric acid). Induced. Rats were exposed to 1 M citric acid for 10 minutes and the cough generated at this time was measured for 10 minutes.
표 1에서 나타낸 바와 같이 황칠 잎 열수 추출물의 진해 활성을 측정한 결과, 시트르산으로 기침을 유발한 대조군에 비해 20.1 % 우수한 진해활성을 확인하였다.As shown in Table 1, as a result of measuring the antitussive activity of the hot-water extract of Hwangchil leaves, it was confirmed that the antitussive activity was 20.1% superior to that of the citrate-induced cough.
표 1 황칠 잎 열수 추출물의 진해 활성 측정결과를 나타낸다.
투여용량(mg/kg) 기침횟수(회/min) 기침억제율(%)
대조군 - 110.2±17.3
실험군(황칠 열수 추출물) 200 88.0±4.7 20.1
Table 1 The results of the antitussive activity of the hot-water extract of Hwangchil leaves are shown.
Dose (mg / kg) Cough Count (times / min) Cough suppression rate (%)
Control - 110.2 ± 17.3
Experimental group (Hwangchil hot water extract) 200 88.0 ± 4.7 20.1
3.5. 피부감작실험을 통한 황칠 잎 열수 추출물, 분획물의 allergy 실험3.5. Allergy experiment of Hwangchil Leaf hot water extract and fractions through skin sensitization test
황칠 잎 열수 추출물 및 분획물의 안전성을 측정하기 위하여 30gauge 인슐린 주사기로 암컷 nude 마우스 등에 황칠 잎 열수 추출물을 10, 50, 70 및 100 mg/mL 농도로 각각 0.02mL 씩 피하 주입하였으며, 황칠 잎 열수 추출물의 용매별 분획물은 각각 50mg/mL 농도로 0.02mL 피하로 주입하였다. 30분 후 황칠 잎 열수 추출물과 황칠 잎 열수 추출물의 용매별 분획물의 allergy 유무를 판단하였다. In order to measure the safety of the hot yellow leaf extract and fractions, the male nude mice were injected subcutaneously with 0.02 mL of 10, 50, 70, and 100 mg / mL concentrations in female nude mice, respectively. Fractions for each solvent were injected subcutaneously at 0.02 mL at 50 mg / mL concentration, respectively. After 30 minutes, the presence or absence of allergy of the fractions by solvent of the Hwangchil leaf hydrothermal extract and the Hwangchil leaf hydrothermal extract was determined.
도 6은 황칠 잎 열수 추출물 및 황칠 열수 추출물의 용매별 분획물의 피부감작실험 동물모델에서의 allergy 결과를 나타낸다. 도 6A에서 나타낸바와 같이, 황칠 잎 열수 추출물을 농도 (10, 50, 70, 100 mg/mL) 별로 처리해서 확인한 결과 50mg/mL 농도 (최종농도 1mg) 이하에서는 알러지 반응이 유발되지 않는 것을 확인하였다(표 2). Figure 6 shows the results of allergy in the skin sensitization test animal model of the solvent-specific fractions of the Hwangchil hydrothermal extract and Hwangchil hydrothermal extract. As shown in FIG. 6A, the results were confirmed by treating Hwangchil leaf hydrothermal extract by concentration (10, 50, 70, 100 mg / mL) and confirming that the allergic reaction was not induced at the 50 mg / mL concentration (final concentration 1 mg) or less. (Table 2).
표 2 황칠 잎 열수 추출물의 농도별 알러지 반응 결과
황칠 물추출물(mg/mL)
10 50 70 100
Allergy 반응 - - ** **
TABLE 2 Allergic Reaction Results by Concentration of Hwangchil Leaf Hot Water Extract
Hwangchil Water Extract (mg / mL)
10 50 70 100
Allergy reaction - - ** **
Allergy 반응: -: 없음, *: 하, **: 중, ***: 상Allergy Reaction:-: None, *: Lower, **: Medium, ***: Upper
다음으로 도 6B에서 나타낸 바와 같이 황칠 잎 열수 추출물의 용매별 분획물(50mg/ml)의 경우, 헥산, 클로로포름, 물 분획물에서는 마우스 피부에 알러지 반응이 나타나지 않음을 확인하였다(표 3). Next, as shown in Figure 6B for the solvent-specific fraction (50mg / ml) of the Hwangchil leaf hydrothermal extract, it was confirmed that the allergic reaction does not appear in the mouse skin in hexane, chloroform, water fraction (Table 3).
표 3 황칠 잎 열수 추출물의 용매별 분획물(50mg/ml)의 경우 알러지 반응 결과
황칠 분획물(50 mg/mL)
헥산 클로로포름 에틸아세이트 부탄올 물
Allergy 반응 - - ** ** -
TABLE 3 Allergic Reaction Results of Solvent Fractions (50mg / ml)
Hwangchil fraction (50 mg / mL)
Hexane chloroform Ethyl acetate Butanol water
Allergy reaction - - ** ** -
Allergy 반응: -: 없음, *: 하, **: 중, ***: 상 Allergy Reaction:-: None, *: Lower, **: Medium, ***: Upper
따라서, 본 발명에서는 황칠 잎 열수 추출물 50mg/mL 농도 (최종농도 1mg), 황칠 잎 열수 추출물의 헥산, 클로로포름, 물 분획물 각각 50mg/mL 농도 (최종농도 1mg)에서 피부에 대한 알러지 반응이 유발되지 않을 정도로 독성이 없이 안정함을 확인하였다. 특히, 클로로포름 분획물은 진해 또는 거담 활성에 안정성이 있는 유효성분이 포함되어 있는 것을 확인하였다. Therefore, in the present invention, allergic reactions to the skin will not be induced at 50 mg / mL concentration (final concentration of 1 mg), hexane, chloroform, and water fractions of 50 mg / mL (final concentration of 1 mg) of yellow lacquer leaf hydrothermal extract (final concentration 1 mg). It was confirmed that it is stable without toxicity. In particular, the chloroform fraction was confirmed to contain an active ingredient that is stable to antitussive or expectorant activity.
이로부터 본 발명의 진해 또는 거담용 의약조성물은 천연원료를 이용하여 독성 및 부작용 없으며 진해 또는 거담제거 작용에 뛰어나기 때문에 이를 유효성분으로 함유하는 건강보조식품으로 복용이 용이하며, 장기간 복용하여도 부작용이 없어 안전하게 사용될 수 있다.From this, the antitussive or expectorant pharmaceutical composition of the present invention is easy to take as a health supplement containing the active ingredient because it is excellent in removing the antitoxicity and antitussive effect by using natural raw materials and without the antitussive or expectorant effect, even if taking long-term side effects It can be used safely without this.
진해 또는 거담용 조성물로서 황칠 잎 열수 추출물은 인체에 부작용이 없으며 진해 또는 거담제거 작용에 뛰어나기 때문에 이를 유효성분으로 함유하는 약학조성물 또는 건강보조식품으로 복용이 용이하며, 장기간 복용하여도 부작용이 없어 안정하게 사용될 수 있고, 진해 또는 거담 제거를 위한 원료를 자연에 서식하는 식물로 대체함으로 제조생산단가 절감과 산업화를 통한 수입대체 및 수출효과를 기대할 수 있을 것이다.Hwangchil leaf hot water extract as a composition for Jinhae or expectorant has no side effects to the human body and is excellent in removing antitussive or expectorant, so it is easy to take as a pharmaceutical composition or health supplement containing it as an active ingredient, and no side effects even after long-term use It can be used stably, and by replacing raw materials for removal of Jinhae or expectorant with plants that live in nature, it is expected to reduce the production cost and import substitution and export effect through industrialization.

Claims (10)

  1. 황칠 잎 조추출물을 유효성분으로 포함하는 진해 또는 거담용 약학 조성물Jinhae or expectorant pharmaceutical composition comprising crude extract as an active ingredient
  2. 제1항에 있어서, 상기 황칠 잎 조추출물은 물, 메탄올, 에탄올, 프로판올, 이소프로판올, 부탄올 또는 이들의 혼합용매 중에서 선택되는 어느 하나로부터 가용한 추출물인 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물According to claim 1, wherein the rough extract of the hwangchil leaves Jinhae containing the hot water extract of hwangchil leaves, characterized in that the extract available from any one selected from water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof Or expectorant pharmaceutical composition
  3. 제1항 또는 제2항에 있어서, 상기 조성물은 0.01 내지 99.9 중량%의 양으로 포함되는 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물According to claim 1 or 2, wherein the composition is an antitussive or expectorant pharmaceutical composition containing the hwangchil leaf hydrothermal extract, characterized in that it is included in an amount of 0.01 to 99.9% by weight
  4. 제3항에 있어서, 상기 추출물의 1일당 투여량은 체중 kg당 10 내지 2000mg/kg의 양으로 음용하는 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물According to claim 3, wherein the daily dose of the extract is an antitussive or expectorant pharmaceutical composition containing hot-lipped leaf hydrothermal extract, characterized in that drinking in an amount of 10 to 2000mg / kg per kg body weight
  5. 제2항에 있어서, 상기 추출용매를 사용하여 추출한 황칠 잎 조추출물에 비극성용매로서 헥산, 클로로포름, 디클로메탄 및 에틸아세테이트 중 어느 하나를 분획용매로 사용하여 분획한 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물According to claim 2, Hwangchil leaves hot water extract, characterized in that the crude extract extracted using the solvent extracted from any one of hexane, chloroform, dichloromethane and ethyl acetate as a fraction solvent as a non-polar solvent Pharmaceutical composition for antitussive or expectorant containing
  6. 제5항에 있어서, 분획물은 전체 조성물의 0.01 내지 99.9중량%로 포함되는 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물The pharmaceutical composition for antitussive or expectorant of claim 5, wherein the fraction comprises 0.01 to 99.9% by weight of the total composition.
  7. 제6항에 있어서, 상기 진해 또는 거담용 약학 조성물의 1일당 투여량은 상기 분획물이 체중 kg당 1 내지 1000 mg/kg의 함량인 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물The pharmaceutical composition for antitussive or expectorant according to claim 6, wherein the daily dose of the pharmaceutical composition for antitussive or expectorant is a content of 1 to 1000 mg / kg per kg body weight of the fraction. Composition
  8. 제4항 또는 제7항에 있어서, 상기 조성물은 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽, 에어로졸, 경피제, 좌제 또는 멸균 주사용 액으로 제형화된 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물8. Hwangchil leaf hot water according to claim 4 or 7, wherein the composition is formulated as a powder, granule, tablet, capsule, suspension, emulsion, syrup, aerosol, transdermal, suppository, or sterile injectable solution. Pharmaceutical composition for antitussive or expectorant containing extract
  9. 제1항 내지 제7항 중 어느 한 항에 있어서, 상기 추출물은 호흡기 질환의 예방 또는 치료용 약학 조성물로 사용되는 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물The pharmaceutical composition for antitussive or expectorant of claim 1, wherein the extract is used as a pharmaceutical composition for the prophylaxis or treatment of respiratory diseases.
  10. 제9항에 있어서, 상기 호흡기 질환은 폐기종, 만성기관지염, 기관지선종, 고립성폐결절, 폐결핵, 농흉, 폐농양, 감기, 독감 또는 폐의 조직구 증식증인 호흡기 질환의 예방 또는 치료용 약학 조성물로 사용되는 것을 특징으로 하는 황칠 잎 열수 추출물을 함유하는 진해 또는 거담용 약학 조성물The method of claim 9, wherein the respiratory disease is used as a pharmaceutical composition for preventing or treating respiratory diseases such as emphysema, chronic bronchitis, bronchial adenomas, isolated pulmonary nodules, pulmonary tuberculosis, empyema, lung abscess, cold, flu, or histiocytosis of the lung. Jinhae or expectorant pharmaceutical composition containing hot-water extract of yellow lacquer leaf, characterized in that
PCT/KR2014/000680 2013-02-04 2014-01-23 Pharmaceutical composition for cough relief or discharge of phlegm, containing hot water extract of dendropanax morbiferus h.lev. leaves WO2014119875A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104958395A (en) * 2015-08-02 2015-10-07 闫花英 Traditional Chinese medicine composition for treating cold-with-dampness type cough

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101490030B1 (en) 2013-07-02 2015-02-06 재단법인 전남생물산업진흥원 Cigarette filter FOR ANTI-TUSSIVE ACTIVITY OR DISCHARGE OF PHLEGM ACTIVITY COMPRISING EXTRACTS OF DENDROPANAX MORBIFERA
KR102413705B1 (en) 2014-05-29 2022-06-27 주식회사유한양행 Composition for improving liver function comprising Dendropanax morbifera extract
KR102624473B1 (en) * 2019-07-31 2024-01-15 (주)화인내츄럴 A Manufacturing method of sliced bellflower sugared
KR102359165B1 (en) 2019-08-01 2022-02-08 비알 컨설팅 인코포레이티드 Dendropanax morbifera complex extracts for improving liver and kidney, Manufacturing method thereof and Health functional food containing the same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20000004499A (en) * 1998-06-30 2000-01-25 박호군 Dendropanax morbifera lev extract having anti-cancer activity
KR20040097112A (en) * 2004-11-01 2004-11-17 주식회사 디피바이오텍 A health foodstuff having a effective component a Dendropanax morbifera Lev. extract, Dendropanax morbifera Lev. fractions
KR20060131360A (en) * 2005-06-16 2006-12-20 제주대학교 산학협력단 The fruits extracts of dendropanax morbifera leveille having the excellent biological activity
KR20110067789A (en) * 2009-12-15 2011-06-22 한국인스팜(주) A composition comprising the extract of crude drug selected from eriobotryae folium and dendropanax morbiferafor treating and preventing neuro-degenerative disease
KR20120101743A (en) * 2011-02-17 2012-09-17 동아대학교 산학협력단 Composition for treating and preventing diabetes containing dendropanoxide from leaves of dendropanax morbifera leveille

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100494482B1 (en) * 2002-04-02 2005-06-10 주식회사 디피바이오텍 Dendropanax morbifera Lev. extract having a protective effect of hepatic cells, Dendropanax morbifera Lev. fractions, and pharmaceutical composition containing the same
KR101313178B1 (en) * 2011-04-20 2013-09-30 경희대학교 산학협력단 Composition for skin-whitening including phenolic compound isolated from extracts of dendropanax morbifera

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20000004499A (en) * 1998-06-30 2000-01-25 박호군 Dendropanax morbifera lev extract having anti-cancer activity
KR20040097112A (en) * 2004-11-01 2004-11-17 주식회사 디피바이오텍 A health foodstuff having a effective component a Dendropanax morbifera Lev. extract, Dendropanax morbifera Lev. fractions
KR20060131360A (en) * 2005-06-16 2006-12-20 제주대학교 산학협력단 The fruits extracts of dendropanax morbifera leveille having the excellent biological activity
KR20110067789A (en) * 2009-12-15 2011-06-22 한국인스팜(주) A composition comprising the extract of crude drug selected from eriobotryae folium and dendropanax morbiferafor treating and preventing neuro-degenerative disease
KR20120101743A (en) * 2011-02-17 2012-09-17 동아대학교 산학협력단 Composition for treating and preventing diabetes containing dendropanoxide from leaves of dendropanax morbifera leveille

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104958395A (en) * 2015-08-02 2015-10-07 闫花英 Traditional Chinese medicine composition for treating cold-with-dampness type cough

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