KR101490030B1 - Cigarette filter FOR ANTI-TUSSIVE ACTIVITY OR DISCHARGE OF PHLEGM ACTIVITY COMPRISING EXTRACTS OF DENDROPANAX MORBIFERA - Google Patents

Abstract

The present invention relates to a process for the production of a non-polar soluble one of hexane, chloroform, dichloromethane and ethyl acetate as a nonpolar solvent in a crude leaf extract extracted from water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof, The present invention also provides a method for producing a tobacco filter having an extractive activity as an active ingredient and having a shrinking or deodorizing activity.
Since the medicinal composition for deep-sea or dandruff of the present invention is free from toxicity and side effects by using natural raw materials, it is excellent in the action of removing the deep sea bream or the ginger dander, so it is easy to produce a tobacco filter containing it as an active ingredient. .

Description

TECHNICAL FIELD The present invention relates to a cigarette filter containing an extract of Huangchil Leaf having an active ingredient and a method for producing the same,

The present invention relates to a tobacco filter containing a Huangchil-derived extract, which is a natural product, as an active ingredient, and a Huangchil leaf extract having the same activity, and more particularly, Can be safely used without toxicity and side effects and can be safely used without any adverse effects. Also, it can be used safely without any toxic effect or side effects, and can be used safely without any adverse effects even when taken for a long time. And a method of manufacturing the same.

It is an economic value of traditional Korean paintings. It is a traditional Korean traditional painting, and it has economic values such as Wando, Haenam, and Bogildo, and the southern coast of Jeollanamdo, It is an evergreen broad-leaved arboreous tree native to a large area.

The woody tree is 15 m high, and the young branches are green and have no hairs. The leaves of the perennials are alternate ovate or oval, and the flowers bloom in June with light yellow green color and are positive. The fruit of the perennial tree is elliptical and opens in black in October. The juice of the above-mentioned Hwangchil tree which is used for the traditional craft, Huangchil, is collected from August to September.

In winter, it is a species that does not support fallen leaves. If you bruise the bark, yellow resinous liquid comes out and it is called 黄 漆. Hwangchil is a transparent natural paint that has been used in furniture, leather, cloth, metal, paper and so on since the Three Kingdoms period. Especially, wood crafts with yellow crust have the advantage of brightening the wood grain of the base, It has been widely used in woodworking.

In addition to its function as a paint, Huangchil is known to have some pharmacological action. Huangchil in Huangchil, which was written by Ming Yi, has been known to eliminate the burning, to alleviate yellow jaundice and yellowing of the liquor, and to treat leprosy .

The ingredients of Huangchil are composed of 66.7% of nonvolatile components, 10.8% of directional components, 8.1% of water and 14.4% of solids, which is a paint component that forms a golden coat. The directional component is mainly composed of cubebene, γ-selinene and δ-cadinene of sesquiterpenes, and these components are known to exhibit sedative and tonic effects on the nervous system .

In addition, when the sap is grown properly, it cleanses the head and makes the body feel comfortable. It is also used as a fragrance for women because it has a charming and fascinating fragrance. It is also used as an antioxidant (antioxidant, pterostyrax hispidum ), And it is known that the flavor evolved from yellowing products is unique as it was made with benzylic acid solution.

In particular, when it is known that it has antioxidative, antibacterial or anticancer effects, it can be used as a medicine in case of separating certain components from Huangchil, and it is easy to handle without causing skin allergy almost unlike lacquer, It can be widely used as a health food.

Considering the industrialization using Hwangchu-gil leaf, it is much more economical when using Hwangchu-gil leaf (based on raw leaves), which is sold about 12,000 to 25,000 won per kg, compared to the sap that is sold for 90,000 to 100,000 won per g Able to know.

However, some of the components of sesquiterpene, which is the major component of the crude oil, are not only unique but also bitter. , Jinhae or geomorphic studies are not known at all.

In order to solve such problems, the present invention can be safely used without toxicity and side effects by using hot water extract of Huangchil leaf, which is a natural raw material which is more economical than juvenile juice, as an active ingredient. A tobacco filter containing a Huanghui leaf extract having a chewing gum activity and a preparation method thereof.

Korean Patent Laid-Open Publication No. 10-2011-0091959 discloses a plant which is effective in anticancer and cancer treatment in traditional oriental medicine and private medicine, such as lacquer tree, Hwangchil tree, fucoidan (algae such as seaweed), chitosan It is possible to destroy the marine ecosystem by using ordinary water such as silkworm grass, chestnut, chestnut, garlic, saururus chrysanthemum, chrysanthemum, A health functional food composition and method for treating and improving cancer by using apoptosis inducing action of killing normal cancer cells by using a fermenting liquid obtained by fermenting with lactic acid bacterium using only an extract of jellyfish designated as a disturbing animal ≪ / RTI > Korean Patent Laid-Open Publication No. 10-2011-00801387 discloses a composition for hair which further comprises extracts obtained from at least one selected from the group consisting of red ginseng extract, The present invention relates to an additive, and more particularly, to a hair composition comprising a extract of Hokkaido japonica, which provides an effect of protecting hair and scalp by preventing skin irritation and dermatitis when a hair dye is used to increase the concentration of an extract, Additives and a process for producing the same. Korean Patent Laid-Open Publication No. 10-2007-0098529 discloses a method of sterilizing and detoxifying a mixture of charcoal powder, nano-silver powder, elvan powder, and ginkgo powder with a conventional yellowish liquid which has no function of sterilizing bacteria or detoxifying harmful components And a method for manufacturing a functional yellowing that has a sterilizing and decontaminating function for producing a functional yellowing having a preservative function. Korean Patent Laid-Open Publication No. 1998-026683 discloses a solvent composition for yellow paint, which is a gold-colored natural paint prepared by collecting sap from woody wood. More specifically, a solvent selected from esters, ethers and ketone solvents is used alone Or mixed to form a solution for the purpose of controlling the solubility, flowability and viscosity of the emulsion, and a solvent for the purpose of controlling the solubility or the rate of evaporation of the emulsifier is used, and a hydrocarbon solvent is used for dilution Discloses a solvent composition for painted paint. However, in this prior art, as in the present invention, it can be safely used without toxicity and side effects by using the hot water extract of Huangchil leaf which is a natural raw material as an active ingredient, and it can be used safely without any adverse effect even if it is taken for a long time, This method is different from the tobacco filter containing Huangchil leaf extract which has gingerbreading activity and its preparation method to enable phloem removal (germination).

The present invention relates to a tobacco filter containing a Huangchil leaf extract which has an effective action on Jinhae or a phagocytosis activity and a Huanghui leaf extract having a phagocytosis activity and a method for preparing the same. It is intended to provide a cigarette filter that works to reliably prevent deep darkness or giddiness without side effects.

In order to solve the above-mentioned problems, the present invention provides a tobacco filter for deep sea or dwarf, comprising an extract of Huangchil Leaf or a non-polar soluble extract as an active ingredient.

The Crassulaceae leaf extract may be an extract which is soluble in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof. To the Crassulaceae leaf extract extracted with the above extracting solvent, hexane, chloroform , Fractionation using either dichloromethane or ethyl acetate as the fraction solvent.

The pharmaceutical composition for oral hygiene or dandruff comprising 0.01 to 99.9% by weight of the pharmaceutical composition for chewing gum or dandruff characterized by containing extracts soluble in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof as an active ingredient And the daily dose of the extract can be taken in an amount of 10 to 2000 mg / kg per kg of body weight.

The pharmaceutical composition for deep sea or dandruff, which comprises as an active ingredient a fraction obtained by using the crude extract of Huangchil leaf as a fraction solvent of any one of hexane, chloroform, dichloromethane and ethyl acetate as a non-polar solvent, %, And the daily dose of the extract may be administered in an amount of 1 to 1000 mg / kg of the fraction per kg of body weight.

More preferably, the fraction is a fraction obtained by fractionation of chloroform-derived hot-water extract of Yellow Grass leaf as an active ingredient. The fraction contains 0.01 to 99.9% by weight of the total composition, and the yellowish leaf The daily dose of the hot-water extract is such that the fraction contains 1 to 1000 mg / kg body weight.

The cigarette filter is characterized in that it is made of at least one selected from a filter for electronic cigarette, an acetate filter, a paper filter, a charcoal filter, and a carbon filter.

Cigarette filter S1) drying the perennial leaf; S2) hot water extracting the leaf of Tochigi; S3) lyophilizing the hot-water extract of step S2) to obtain a concentrated hot-water extract; S4) spraying the hot-water extract powder of Hwangbukki leaf in the step S3) into a tobacco filter type; .

S1) It is dried at 40 ~ 80 degrees Celsius,

S2) and S3) was washed with distilled water, and then 20 L of distilled water was added thereto. The resultant was lyophilized by heating at 100 ° C. for 3 hours in an electric bath,

The tobacco filter used in the step S4) is any one selected from a cellulose filter, a double charcoal filter and a triple charcoal filter. The powder is obtained by diluting a hot water extract powder of Hwangchilgae leaf with water at a concentration of 5 ~ 15% Spraying the tobacco filter with 20 to 50 ul, and drying the tobacco filter in a 50 to 60 oven for 1 to 2 hours.

The present invention can be used for cough removal or sputum elimination (genomic) without adverse effect even if taken for a long period of time by using hot water extract of Huangchil leaf as a natural ingredient in a cigarette filter. The chloroform fraction of Huanghui leaf hot water extract By using it as an active ingredient, there is a remarkable effect that the cough can be eliminated (shrinkage) or sputum elimination (gossamer) without side effects even when taken for a long time.

Fig. 1 is a photograph showing a yellowish leaf.
FIG. 2 shows the extraction and fractionation diagram of the hot-water extract of Yellow Grass leaf.
Fig. 3 shows the results of measurement of mucous secretion amount in airway to the hot-water extract of Yellow Grass leaf.
Fig. 4 shows the results of measurement of mucous secretion amount in airway of the hot-water extract and the methanol extract of Yellow Grass leaf.
FIG. 5 shows the result of measurement of mucous secretion amount in the airway for the fraction of the solvent of the hot-water extract of Yellow Grass leaf.
FIG. 6 shows the results of allergy in an animal model of skin sensitization of the fractions of the solvent of the hot water extract of Huangchil leaf and the Huanghe hot water extract.
FIG. 7 is a schematic diagram of a tobacco filter containing a hot water extract of Yellow Grass leaf
FIG. 8 shows the results of HPLC separation of the hot-water extract of Yellow Grass leaf.
FIG. 9 shows the result of separating a substance obtained by capturing a tobacco filter with a methanol solvent by HPLC.
FIG. 10 shows the results obtained by separating a material obtained by passing hot water extract of Huangchil leaf through a cigarette filter and collecting it with a solvent by HPLC.

The present invention provides a tobacco filter for deep sea or dwarf, comprising as an active ingredient an extract of Yellow Grass leaf or a non-polar soluble extract.

The Crassulaceae leaf extract may be an extract which is soluble in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof. To the Crassulaceae leaf extract extracted with the above extracting solvent, hexane, chloroform , Fractionation using either dichloromethane or ethyl acetate as the fraction solvent.

In addition, the extract may be provided in an amount of 0.01 to 99.9% by weight of the whole composition and provided as a filter for deep sea or dwarfism, and the daily dose of the extract may be 10 to 2000 mg / kg per kg of body weight .

Wherein the fractions obtained by fractionating the hot water extract of Huangchil leaf with chloroform are contained as an active ingredient and the fractions are contained in an amount of 0.01 to 99.9% by weight of the total composition, and the daily dose of the composition contained in the tobacco filter for deep- And the fraction contains 1 to 1000 mg / kg body weight.

A method for extracting a composition for a deep sea or a goddam includes the steps of washing watery leaves with distilled water, mixing with distilled water, Filtering and concentrating the extract to freeze-dry the extract; And extracting the extract of step (a) by dissolving the extract in a fraction solvent, filtering and concentrating the extract with a vacuum filtration apparatus to obtain a fraction, and freeze-drying the extract.

The extract is used as a filter for preventing or treating a respiratory disease, and the composition is formulated into a powder, granule, tablet, capsule, suspension, emulsion, syrup, aerosol, transdermal preparation, Which is used for the prevention or treatment of respiratory diseases.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

One. Burrows  leaf Heat number  The extract and Fraction  Produce

FIG. 2 shows the process of obtaining a hot-water extract of Yellow Grass leaf and a fraction by an organic solvent. 1 kg of dried yellowish crushed with water was washed with distilled water and then 20 L of distilled water was added thereto. The mixture was heated and extracted at 100 캜 for 3 hours with an electric bath.

The extracted solution was filtered with 400 mesh filter cloth and then concentrated with a rotary evaporator under reduced pressure. After the filtration, the same amount of distilled water is again used for the remaining residue, which is further extracted twice, filtered and concentrated under reduced pressure. The concentrated hot water extract was lyophilized in a freeze dryer. After drying, 279g (24.9%) of hot-water extract of Yellowish leaves was obtained.

2. Preparation of polar solvent and nonpolar solvent-soluble fraction of Huangchil leaf

The fractions were prepared using the organic extracts of the Yellowish-leaf hot-water extract prepared as shown in FIG. For the preparation of the polar solvent and the nonpolar solvent soluble fraction of Huangchil leaf, 249g of Huangchil leaf hot water extract was completely dissolved in 1L of distilled water, and the water layer and the hexane layer were separated by adding 1L of hexane (Hexane) Was repeated three times.

Each fraction was obtained by sequentially adding chloroform, ethyl acetate, butanol, and water through the same procedure. Each of the obtained fractions was filtered through a vacuum filtration apparatus, concentrated and lyophilized to obtain a solvent Were completely removed and used in this experiment.

2.1. Hexane-soluble fraction separation

249g of Huangchil leaf hot water extract was completely dissolved in lL of distilled water, and then put into a separating funnel. 1L of hexane was added to separate the hexane insoluble layer (water layer) and the hexane soluble layer. Again, the hexane insoluble fraction and the soluble fraction were collected by repeating the same process three times for the hexane insoluble layer (water layer).

2.2. Chloroform-soluble fractionation

The hexane insoluble fraction (aqueous layer) was mixed with 5 L of chloroform, and then the chloroform-soluble fraction and the insoluble fraction were separated. The chloroform-insoluble fraction and the soluble fraction were collected by repeating the same process three times for the chloroform insoluble layer (water layer).

2.3. Ethyl acetate soluble fraction separation

After adding 5 L of ethyl acetate to the chloroform insoluble fraction (aqueous layer), the ethyl acetate soluble fraction and the insoluble fraction were separated. The ethyl acetate insoluble layer (water layer) was subjected to the same process three times to collect the ethyl acetate insoluble fraction and the soluble fraction Respectively.

2.4. Butanol soluble fraction fractionation

The butanol insoluble fraction and the insoluble fraction were separated by adding 5 L of butanol to the ethyl acetate insoluble fraction (water layer), and the butanol insoluble fraction was repeated three times to collect the butanol insoluble fraction and the soluble fraction.

2.5. Obtained Hot Water Extract and Fraction of Yellow Grass Leaves

The hexane-soluble fraction, the chloroform-soluble fraction, the ethyl acetate-soluble fraction and the butanol-soluble fraction were concentrated under reduced pressure and the residue was purified by filtration to obtain 0.096 g (0.04%) of hexane fraction, 0.57 g (0.84%) of chloroform fraction, , 4.04 g (1.63%) of the fraction, 54.74 g (10.28%) of the butanol fraction and 168.64 g (67.84%) of the water fraction were used as a sample

3. Experimental animal breeding

Male ICR mice (18-22 g) and male SD Rat rats weighing 320 ± 100 g at 8 weeks of age were randomly divided into three groups as follows: SAMTACO, Korea, And were adapted for one week under constant conditions (temperature: 22 ± 2 ° C, humidity: 50 ± 5%, darkness: 12 hours light / dark cycle).

3.1. Mucin secretion measurement in airway using phenol red (Hwangchil leaf hot water extract)

Male ICR mice were divided into three groups as normal, positive control, and sample groups in order to measure the gonadal activity of hot water extract of Hwangryuck leaves. The normal group was orally administered 100 μL of saline, and the positive control group was orally administered 200 μL of ammonia chloride (NH 4 Cl) at a concentration of 1000 mg / kg.

In the experimental group, 100 μL of 50, 100, 200 mg / kg of hot water extract of Hwangchil were administered orally. The samples were prepared by dissolving in saline, and NH4Cl was dissolved in third distilled water. One hour after oral administration of each group, 0.2 ml of phenol red (10 mg / kg) was intraperitoneally injected.

Each group of mice was anesthetized with diethylether for 30 minutes and the abdominal aorta was dissected and the entire trachea was excised. Separated organs were placed in 1 ml of physiological saline and the tissues were washed and vortexed for 30 minutes. Then, the washings were centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml of the separated supernatant was taken, and 0.1 ml of 1 M NaOH was added to the solution to develop color. The solution was measured at an absorbance of 546 nm to measure the phenol red extracted from the airways. The ratio of phenol red to airway was divided by the weight of the extracted airway, and the amount of mucous secretion in airway was obtained.

As shown in FIG. 3, the content of phenol red per gram of airway was 2.436 μg / g in the normal group, 3.476 μg / g in the NH4Cl group used as the positive control group, (21.4%), 3.108 (27.6%) and 3.105 μg / g (27%) were obtained in the groups treated with 50, 100 and 200 mg / kg of extracts, respectively. It was confirmed that the hot water extract of Huangchil leaf exhibited an activity (gadotactic activity) superior in the sputum emission ability by increasing the phenol red content, that is, the mucous secretion amount in a concentration dependent manner.

3.2. Measurement of mucus secretion in airway using phenol red (Comparison of hot water extract and methanol extract of Huangchil leaf)

Male SD Rat mice were divided into three groups as normal, normal control, and sample groups to determine the vigorous activity of hydrothermal extract. The normal group was orally administered 500 μL of saline, and the positive control group was orally administered 1 mL of ammonia chloride (NH 4 Cl) at a concentration of 1000 mg / kg.

In the experimental group, 500 μL of 200 mg / kg of water extract of Huangchil leaf and methanol extract of Huangchil leaf were orally administered. The sample was dissolved in saline and NH 4 Cl used in the positive control was dissolved in the third distilled water. After 30 minutes of oral administration, 0.5 mg of phenol red (10 mg / kg) was intraperitoneally injected into each group.

The rats in each group were anesthetized with diethylether for 30 minutes, and the abdominal aorta was dissected to remove the trachea. The separated organs were placed in 1 ml of physiological saline and the tissues were washed and vortexed for 30 minutes, and then the washings were centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml of the separated supernatant was taken, and 0.1 ml of 1 M NaOH was added to the solution to develop color. The solution was measured at an absorbance of 546 nm to measure the phenol red extracted from the airways. The ratio of phenol red to airway was divided by the weight of the extracted airway, and the amount of mucous secretion in airway was obtained.

As shown in Fig. 4, the content of phenol red was 2.6 μg / g in the normal group, 3.9 μg / g in the NH4Cl group used as the positive control group, and 200 mg (19.2%) and 2.2μg / g (no effect), respectively, in the group treated with methanol extract (200mg / kg) It was confirmed that the extract of Hwangyil leaf showed an activity (spontaneous activity) of sputum excretion by increasing the content of phenol red, ie, mucous secretion, than the methanol extract.

3.3. Measurement of mucus secretion in airway using phenol red (Comparison of fraction of solvent in hot water extract of Hwangchil leaf)

Male SD Rat mice were divided into 6 groups as normal, normal control, and sample groups in order to measure the vigorous activity of hydrothermal extract. The normal group was orally administered 500 μL of saline and the positive control group was orally administered 1 mL of ammonia chloride (NH 4 Cl) at a concentration of 1000 mg / kg.

In the experimental group, 500 μL of hot water extract of hexane, chloroform, ethyl acetate, butanol and water fractions were respectively administered at a concentration of 50 mg / kg. The samples were prepared by dissolving in saline and ethanol. NH 4 Cl used in the positive control group was diluted with tertiary distilled water . One hour after oral administration of each group, 0.5 mg of phenol red (10 mg / kg) was intraperitoneally injected.

The rats in each group were anesthetized with diethylether for 30 minutes, and the abdominal aorta was dissected to remove the trachea. The separated organs were placed in 1 ml of physiological saline and the tissues were washed and vortexed for 30 minutes, and then the washings were centrifuged (10,000 rpm, 5 min, room temperature). 0.9 ml of the separated supernatant was taken, and 0.1 ml of 1 M NaOH was added to the solution to develop color. The solution was measured at an absorbance of 546 nm to measure the phenol red extracted from the airways. The ratio of phenol red to airway was divided by the weight of the extracted airway, and the amount of mucous secretion in airway was obtained.

As shown in FIG. 5, the content of phenol red was 2.6 μg / g in the normal group, 3.9 μg / g in the NH ₄ Cl group used as the positive control, (50 mg / kg), the ethyl acetate fraction (50 mg / kg), the butanol fraction (50 mg / kg) and the water layer fraction (50 mg / kg) of the hot water extract of Yellow Grass leaf g / g (84.6%), 3.8μg / g (46.2%), and 2.5μg / g (no significance) and 2.6μg / g (no significance), respectively.

Among them, the phenol red content of the chloroform fraction of the hot water extract of Huangchil leaf was the highest. It was confirmed that the chloroform fraction of Hwangryuck leaf hot water extract showed the best activity (spontaneous activity) of sputum emission by increasing mucous secretion amount.

3.4 Measurement of Vitale Activity of Hot Water Extract of Yellow Grass Leaves Using Citric Acid

In order to investigate the vigorous activity of hydrothermal extracts, male SD Rat rats were divided into two groups, control and sample. Male rats were orally administered Hwangchil water extract (200 mg / kg) and after 1 hour, the rats were placed in a plexe-smear chamber (Buxco, USA) to induce stabilization. Cough inducers (citric acid) Lt; / RTI > Rats were exposed to 1 M citric acid for 10 minutes and cough counts were measured for 10 minutes.

As shown in Table 1, the shrinkage activity of hot water extract of Huangchil leaf was 20.1% higher than that of citrate-induced cough.

The results of the measurement of the vinegar activity of the hot water extract of Huangchil leaf are shown. Dosage (mg / kg) Coughing frequency (times / min) Cough suppression rate (%) Control group - 110.2 ± 17.3 Experimental group
(Huangchun hot water extract) 200 88.0 + - 4.7 20.1

3.5. Allergy experiment of extracts and fractions of Hwangchil leaf by skin sensitization experiment

In order to evaluate the safety of hot water extracts and fractions of Huangchil leaf, hot water extracts of Huangchil leaf were injected subcutaneously in 10, 50, 70 and 100 mg / mL, respectively, into female nude mice with 30 gauge insulin syringe. The fraction of each solvent was injected subcutaneously at a concentration of 50 mg / mL 0.02 mL. After 30 minutes, the presence or absence of allergy was determined on the fraction of the solvent of the hot water extract of Huangchil leaf and the hot water extract of Huangchil leaf.

FIG. 6 shows the results of allergy in an animal model of skin sensitization of the fractions of the solvent of the hot water extract of Huangchil leaf and the Huanghe hot water extract. As shown in FIG. 6A, when the hot water extract of Huangchil leaf was treated at different concentrations (10, 50, 70 and 100 mg / mL), it was confirmed that the allergic reaction was not induced at a concentration of 50 mg / mL (final concentration 1 mg) (Table 2).

Allergic reaction results by concentration of hot water extract Yellowish water extract (mg / mL) 10 50 70 100 Allergy reaction - - ** **

Allergy reaction: -: none, *: lower, **: middle, ***: upper

Next, as shown in FIG. 6B, it was confirmed that in the case of fraction (50 mg / ml) of solvent of hot water extract of Huangchil leaf, no allergic reaction was observed in mouse skin in hexane, chloroform and water fractions (Table 3).

The fraction of solvent (50mg / ml) The yellowish fraction (50 mg / mL) Hexane chloroform Ethyl acetate Butanol water Allergy reaction - - ** ** -

Allergy reaction: -: none, *: lower, **: middle, ***: upper

Therefore, in the present invention, the allergic reaction to the skin is not induced at a concentration of 50 mg / mL (final concentration 1 mg), 50 mg / mL of hexane, chloroform and water fraction of hot water extract of Huangchil leaf And it is stable without toxicity. Particularly, it was confirmed that the chloroform fraction contained an active ingredient which is stable to the deep sea water or gut dam activity.

From this, it can be said that the composition for deep sea or dandruff of the present invention is free of toxicity and side effects by using natural raw materials, and is excellent in the action of removing the deep sea bream or the ginger, so that it can be used as a tobacco filter containing it as an effective ingredient. Can be used safely.

4. Tobacco Leaves on Tobacco Filter Hot water extract  Comparisons to identify substances

The cellulose acetate filter 1 was sprayed with a diluted solution of 12.5% concentration of water extract of Hwangbukki leaf and dried in a 30 oven for 10 hours. The dried cellulose acetate filter was fixed on both sides of a common cellulose acetate filter so as to be a single filter. The fixed filter length is 3 cm and is shown in FIG. In order to confirm whether or not the hot-water extract materials of the leaves of Tochigi leaf had passed through the tobacco filter, the filter was given a constant suction power and the solvent was collected with methanol. To compare, the tobacco filter alone was given a constant suction force, and the solvent was collected with methanol. The extract was prepared. Samples of three conditions were diluted to 10/10 with methanol and analyzed by HPLC using methanol gradient method as shown in Figs. 8, 9 and 10.

As can be seen from FIG. 8, the HPLC data of the hydrothermal extract of Crassulaceae leaf showed a certain pattern of peaks. As shown in FIG. 9, the HPLC data of the tobacco filter alone showed that the retention time At this later point, we were able to identify a specific peak. As shown in FIG. 10, the HPLC data obtained by spraying a diluted solution obtained by diluting hot water extract of Huangchil leaf with water at a concentration of 12.5%, and drying it at 30 oven for 10 hours, It was possible to produce a tobacco filter containing the components having a vigorous or germane activity in the leaves of Yellowish leaf hydrothermal extracts, Respectively.

As a composition for deep-sea or goddess, the hot-water extract of Huangchil leaf has no adverse effects on the human body and is excellent in the action of removing vinegar or dahingam. Therefore, it can be easily taken as a pharmaceutical composition or a health supplement containing an effective ingredient. It can be used stably and it can be expected to reduce the production cost by replacing the raw material for the removal of Jinhae or Gadam shrine with a plant inhabited by nature, and to import substitution and export effect through industrialization.

Claims (12)

Characterized in that it contains an extract of Crassulaceae leaf extract extracted from any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof as an active ingredient,
2. The tobacco filter according to claim 1, wherein the crude Crude leaf crude extract is contained in the tobacco filter in an amount of 10 to 2000 mg / kg body weight per day
Extracts of Yellowish Leaf Extracts Solubilized Extracted from Any One of Water, Methanol, Ethanol, Propanol, Isopropanol, Butanol or a Mixture of These Solvents As a nonpolar solvent, any one of hexane, chloroform, dichloromethane and ethyl acetate is used as a fraction solvent Wherein the fraction obtained by fractionation of the tobacco filter is an effective component,
4. The tobacco filter according to claim 3, characterized in that the fraction is contained in the tobacco filter in an amount of 10 to 1000 mg / kg body weight per day
The cigarette filter according to claim 2 or 4, wherein the cigarette filter is any one selected from a filter for electronic cigarette, an acetate filter, a paper filter, a charcoal filter, a carbon filter, a cellulose filter,
S1) drying the leafy leaves at 40 to 80 degrees Celsius;
S2) hot water extracting the leaf of Tochigi;
S3) lyophilizing the hot-water extract of step S2) to obtain a concentrated hot-water extract;
S4) step S3) is carried out by diluting the hot-water extract powder of Hwanyeolmu leaf with water to a concentration of 5 ~ 15% and spraying the diluted solution to the tobacco filter with 20 ~ 50ul of the tobacco filter. Wherein the cigarette filter is made of a synthetic resin.
[7] The method according to claim 6, wherein steps S2) and S3) are performed by washing the dried Hwigulnyeo leaf with distilled water, adding distilled water thereto, heating the flour at 100 DEG C for 3 hours, Way.
[7] The method of claim 7, wherein the tobacco filter used in step S4) comprises a step of drying the tobacco filter sprayed with the powder in an oven at 50 to 60 DEG C for 1 to 2 hours, , A charcoal filter, a carbon filter, a cellulose filter, a double charcoal filter, and a triple charcoal filter. delete delete delete delete

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