KR101773232B1 - Composition for preventing and treating hangover comprising extracts of hovenia dulcis thunb and extracts of leaves of perilla frutescens britton var - Google Patents

Abstract

The present invention provides a high-value-added functional health food which is effective in relieving hangover, comprising a mixture of Hovenia dulcis extract and tea leaf extract, which is a natural resource of Korea, and can be safely used for long- . In order to achieve the above object, the present invention relates to a mixture of extracts of Hovenia dulcis Thunb extract and tea leaf extract extracted from any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof or a mixture of hexane, chloroform, And ethylacetate as an active ingredient. The present invention provides a hangover-free food composition comprising, as an active ingredient, any one of non-polar soluble extracts selected from the group consisting of: Since the food composition of the present invention for hangover is excellent in the hangover relieving action and excellent in the protection against hepatotoxicity without side effects on the human body, the health supplement containing it as an active ingredient is easy to take and can be stored for a long time, It can be useful for hangover relief and soy sauce protection.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a food composition for prevention and elimination of hangover which comprises a mixture of an extract of Hovenous Fruit and a mixture of Leaf Extract of Charch Leaf. BACKGROUND OF THE INVENTION 1. Field of the Invention [0002]

The present invention relates to a hangover-eliminating food composition, and more particularly to a hangover rescue composition containing a mixture of Hovenous Fruit Extract and Charpy Leaf Extract which can be safely used without toxicity and side effects by using a natural raw material.

Perilla frutescens (L.) Britton var. Acuta (Thunb.) Kudo) is a biennial medicinal plant belonging to the pizza plant door, dicotyledonous plant river, and Lamiaceae family in the biological classification and is distributed in Korea and China.

The stem is straight and square, all the plants are purple and fragrant, the leaves are broad, ovate, the ends are pointed, the bottom is round or somewhat wedge shaped, the edge has sawtooths, It has long hairs on it and a long petiole. Flowers bloom in August to September with light purple color, and hang on to the stem of the stem, the tip of the branch, and the leaf blade of the upper part. The calyx is divided into two, the upper one is again three rows, the lower one is two rows, and there are hairs on the inside and outside of the tubule. The corolla is short, straight, and the bottom is slightly longer than the top, the stamen is round and the fruit is round with a branch. This species is purple throughout, the circle is circular, and there is a net pattern. Leaves and stems are used for medicinal purposes; young leaves and seeds are edible.

Young leaves are hardly distinguishable from perilla leaves, and sugar made from perylaldehyde in charcoal is about 2,000 times stronger than normal sugar, so it is used for tobacco, toothpaste and toothpaste. It is said that the leaves are not purple, but green, and they are called for for viridis. The cleaned leaves are white flowers and smell stronger than those of tea.

The hinoki is 10m high and the bark is dark gray and cracked and peeled vertically. Leaves are alternate phyllotaxis, oval or oval shape, with a frog on the edge. The front side of the leaf is green and has no hairs. The back side is light green with no hairs on the veins. Fruits ripen in September ~ October, rounded, brownish, deciduous broad-leaved arboreous tree, native to Korea and distributed throughout the country.

Drinking is indispensable for modern people. Excessive drinking temporarily causes hangover such as throbbing, headache, thirst, nausea, gastrointestinal disorder, diarrhea, etc., and chronic diseases such as fatty liver and cirrhosis Can cause. Headaches during hangover are due to the expansion of brain blood vessels. Thirst and dehydration are caused by the diuretic effect of ethanol, which is produced by the suppression of the secretion of vasopressin, an antidiuretic hormone secreted by ethanol in the pituitary gland. In addition, digestive organ abnormalities such as gastrointestinal disorders are caused by an increase in gastric acid secretion, and the main cause is toxicity of ethanol or acetaldehyde accumulated in hepatocytes.

In addition to the mechanism of ethanol degradation, toxicological studies of ethanol have been done in various ways. It has been reported that the toxicity of ethanol is not only observed neurologically but also genetically influenced (J. Caballeria, et al Life Sci., 41: 1021-1727, 1986). In recent years, many researches and experiments have been conducted on a number of substances that can reduce the toxicity of ethanol or inhibit the expression of toxins, and as a result, a variety of health supplements containing ingredients extracted from natural or herbal ingredients (Kim JJ, et al., Journal of the Korean Society of Agricultural Chemistry, 38 (6): 549-553, 1995).

Accordingly, the present invention provides a hangover rescue composition containing a mixture of Hovenia dulciflora extract and Leaf leaf extract, which can be safely used without toxicity and side effects by using a mixture of Hovenia dulcis extract and Leaf leaf extract for utilization of traditional plant resources of Korea I want to.

Korean Patent Laid-Open Publication No. 10-2011-0060002 relates to a composition for improving hangover, which comprises an appropriate ratio of post-fermented tea extract and chitooligosaccharide using a non-pathogenic microorganism, and contains an aldehyde dehydrogenase which degrades acetaldehyde, Compositions for the improvement of hangover, reduction of blood acetaldehyde concentration, hepatoprotection and improvement of hangover for fatigue recovery through synergistic effects on the activation of hypertension. Korean Patent Laid-Open Publication No. 10-2011-0033644 relates to a composition for relieving hangover which accelerates blood alcohol degradation rate and a method for producing the same, and more particularly to a composition for removing hangover which comprises an extract of Hovenia dulcis, Artemisia japonica, The present invention relates to a composition for use as a hangover detoxifying composition, and more particularly, to a composition for a hangover detoxifying composition, which shows a high synergistic effect as compared with the case where the respective components are separately used by mixing the components at appropriate ratios, And gastric inflammation. Korean Patent Laid-Open Publication No. 10-2010-0119150 relates to a composition for hangover decomposition containing a herbal medicine mixed extract and is characterized by containing an extract of Utila or an extract of Ufu, pomegranate and kidney as an active ingredient. From this, the activity of ADH and ALDH is promoted and the decomposition of alcohol and acetaldehyde is promoted, so that it can be usefully used as a hangover food and beverage. Korean Patent Publication No. 10-0372561 relates to a composition for hangover marrow containing an extract of natural herbal medicine and a health supplement containing the same as an effective ingredient and is characterized by containing at least one natural selected from the group consisting of red ginseng, red ginseng, And at least one natural herbal medicine extract selected from the group consisting of 40 to 80% by weight of the herbal medicine extract, 5 to 40% by weight of at least one natural herbal medicine extract selected from the group consisting of Rhizobium, Rhubarb, 5 to 40% by weight of the extract, 5 to 15% by weight of at least one natural herbal medicine extract selected from the group consisting of licorice, wormwood, and green tea, and a composition containing the same as an active ingredient ≪ / RTI > However, the above-mentioned prior arts have different materials and ingredients from those of the composition for hulting hangover using a mixture of Hovenia dulcis extract and Leaf leaf extract for the purpose of the present invention.

The present invention provides a high-value-added functional health food which acts as a hangover relieving agent comprising a mixture of Hovenous Fruit extract and Chazju leaf extract, which are natural resources of Korea, and provides safe hangover elimination composition without side effects even when taken for a long time by using natural raw materials I want to.

In order to accomplish the object of the present invention described above, the present invention provides a hangover resolution composition comprising as an active ingredient, a mixture of a horno fruit extract and a tea leaf extract or a non-polar soluble extract thereof.

The Houttuynia fruit extract and the tea leaf extract mixture may be extracted by using at least one selected from the group consisting of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof as an extraction solvent. , And a non-polar solvent which is obtained by fractionating the crude extract of Chamaecyparis obtusa using any one selected from the group consisting of hexane, chloroform, dichloromethane and ethyl acetate as a fraction solvent.

In addition, a drink for prevention and elimination of hangover may be provided, which comprises the extract as an active ingredient, and the extract may be contained in an amount of 0.01 to 100% by weight of the total hangover resolution composition.

Since the composition for the hangover of the present invention is excellent in the effect of eliminating the hangover and excellent in the effect of protecting the hepatotoxicity without side effects on the human body, the health supplement containing it as an active ingredient is easy to take and can be stored for a long time, And can be usefully used for the prevention of liver and liver.

Fig. 1 shows the extraction and fractionation diagrams of the hot-water extract of Chaji leaf.
Fig. 2 shows a schematic diagram of the extraction of the hot-water extract of Hovenia dulcis.
Fig. 3 shows the results of blood alcohol concentration measurement on a mixture of Houttuynia cordata fruit extract and Cha-ki leaf extract.
FIG. 4 is a graph showing a comparison of the blood alcohol concentration with the positive control hanging drink (YM) in the hangover resolution observation experiment of a mixture of Houttuynia cordata fruit extract and Chauzi leaf extract.

The present invention provides a composition for prevention and elimination of hangover comprising a mixture of Hovenous Fruit extract, Charpy Leaf Extract or nonpolar soluble extract as an active ingredient.

The fruit extract of Hovenia dulcis Thunbergii and the tea leaf extract may be obtained by extracting at least one selected from the group consisting of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof as an extraction solvent, The non-polar soluble solvent extracted from the Hovenia dulcis extract and the tea leaf extract may be fractionated using any one selected from the group consisting of hexane, chloroform, dichloromethane and ethyl acetate, and mixtures thereof as a fraction solvent.

The extract mixture may be contained in an amount of 0.01 to 95% by weight of the total composition, and may be provided as a beverage for prevention and elimination of hangover, and the amount of the extract mixture to be provided may be provided in an amount of 100-200 mg per kg of body weight.

A method for extracting a composition for prevention and elimination of hangover comprises the steps of: washing each of Hovenous Fruit and Charan leaf with distilled water, mixing with distilled water, and extracting it with hot water; Filtering and concentrating the extract to freeze-dry the extract; Dissolving the extract of the above step in a fraction solvent to obtain fractions, filtering and concentrating the fractions with a vacuum filter, and freeze-drying the extract.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

1. Preparation of hot water extracts and fractions of Leaf tea

Fig. 1 shows a process for obtaining a tea leaf extract and an organic solvent fraction. 3 kg of water was washed with distilled water, and 30 L of distilled water was added thereto, and the mixture was heated and extracted at 100 캜 for 3 hours with an electric bath.

The extracted solution was filtered with 400 mesh filter cloth and then concentrated with a rotary evaporator under reduced pressure. After the filtration, the same amount of distilled water is again used for the remaining residue, which is further extracted twice, filtered and concentrated under reduced pressure. The concentrated hot water extract was lyophilized in a freeze dryer. And 390 g (13.01%) of hot water extract of Chazugi leaf were obtained.

2. Preparation of polar solvent and non-polar solvent-soluble fraction of tea leaves

The hot-water extract prepared as shown in Fig. 1 was fractionated using an organic solvent. The production of polar and nonpolar solvent soluble fractions of tea leaves 250 g of hot water extract of Chazu leaf was completely dissolved in 1 L of distilled water, and the water layer and the hexane layer were separated by adding 1 L of hexane (Hexane) to the fractional funnel, and this process was repeated three times.

Chloroform, ethyl acetate and butanol were sequentially added through the same procedure to obtain fractions. Each of the fractions thus obtained was filtered with a vacuum filtration apparatus and concentrated. The extract was lyophilized to remove the solvent completely This test was used for this experiment.

2.1. Hexane-soluble fraction separation

After 250 g of the hot water extract of Chazu was completely dissolved in 1 L of distilled water, the mixture was placed in a separating funnel and 1 L of hexane was added to separate the hexane insoluble layer (water layer) and the hexane soluble layer. Again, the hexane insoluble fraction and the soluble fraction were collected by repeating the same process three times for the hexane insoluble layer (water layer).

2.2. Isolation of Soluble Fraction from Chloroform

The hexane insoluble fraction (aqueous layer) was mixed with 5 L of chloroform, and then the chloroform-soluble fraction and the insoluble fraction were separated. The chloroform-insoluble fraction and the soluble fraction were collected by repeating the same process three times for the chloroform insoluble layer (water layer).

2.3. Ethyl acetate soluble fractionation

After adding 5 L of ethyl acetate to the chloroform insoluble fraction (aqueous layer), the ethyl acetate soluble fraction and the insoluble fraction were separated. The ethyl acetate insoluble layer (water layer) was subjected to the same process three times to collect the ethyl acetate insoluble fraction and the soluble fraction Respectively.

2.4. Butanol soluble fraction fractionation

The butanol insoluble fraction and the insoluble fraction were separated by adding 5 L of butanol to the ethyl acetate insoluble fraction (water layer), and the butanol insoluble fraction was repeated three times to collect the butanol insoluble fraction and the soluble fraction.

2.5 Obtained Hot Water Extract and Fraction of Leaf Leaf

The hexane-soluble fraction, the chloroform-soluble fraction, the ethyl acetate-soluble fraction and the butanol-soluble fraction were concentrated under reduced pressure and then lyophilized to obtain 0.015 g of the hexane fraction, 0.57 g of the chloroform fraction, 3.62 g of the ethyl acetate fraction, g was used as a sample.

3. Production of hot-water extract of Hovenia fruit

Fig. 2 shows the process of obtaining houtto fruit extract. One kilogram of the hoven fruit was washed with distilled water, and then 20 L of distilled water was added thereto, and the mixture was heated and extracted at 100 DEG C for 3 hours with an electric bath.

The extracted solution was filtered with 400 mesh filter cloth and then concentrated with a rotary evaporator under reduced pressure. After the filtration, the same amount of distilled water is again used for the remaining residue, which is further extracted twice, filtered and concentrated under reduced pressure. The concentrated hot water extract was lyophilized in a freeze dryer. 298.2 g (29.8%) of hot water extract of Hovenia fruit was obtained.

4. Experimental animal breeding and serum separation

Male ICR mice weighing 15 to 20 g at 5 weeks of age were purchased from SAMTACO, Korea as an experimental animal for the prevention of hangover and the effect of preventing the hangover of a mixture of Houttuynia cordata L. and Chazuri leaf extract. The animals were adapted for one week at a constant temperature (temperature: 22 ± 2 ° C, humidity: 50 ± 5%, darkness: 12 hours light / dark cycle)

4.1 Experimental animal Alcohol administration and serum separation

Six experimental groups were divided into normal (normal), control (control), positive control (control), and sample administration groups. Prior to the experiment, the animals were fasted for 18 hours to exclude the absorption of alcohol through the gastrointestinal tract, which may be caused by feed intake, and water was supplied without restriction.

The mixing ratio of hot water extract of Houttuynia cordata and hot water extract of Chazji leaf was orally administered at a ratio of 1: 0.5, 1: 1 (hot water extract of Houttuynia cordata: hot water extract of Chazju leaf). Specifically, 200 mg / kg of hot water extract of Hovenia duli fruit was mixed with 100 and 200 mg / kg of hot water extract of Chaziri leaf, respectively, orally. In addition, oral administration of 200, 400 mg / kg, and 200 mg / kg (based on the main component) of the extracts of Hovenia duli fruit juice, 200 and 400 mg / kg, respectively.

Alcohol was given once at a level of 3g per kg body weight of 25% alcohol. The control group received oral administration of distilled water instead of the sample and no conditions were given to the normal group. Blood samples were taken at 0.5 hour, 1 hour, 2 hours, and 4 hours after dihydrate ether anesthesia with alcohol. The collected blood was allowed to stand at room temperature for 2 hours, centrifuged at 600 xg for 15 minutes, and the serum was separated to measure the alcohol concentration.

5. Hinoki  Fruit extract Charge  Blood alcohol concentration test on leaf extract mixture

Blood alcohol concentration was measured using the EtOH assay kit (biovision, USA). 5μl of serum isolated from blood was diluted 1:10 with EtOH assay buffer and mixed with 50μl of reaction mixture. After standing for 30 minutes at room temperature in a dark room, the absorbance was measured at 570 nm and the alcohol concentration in the blood was calculated using an ethanol calibration curve.

Hovenia dulcis extract and tea leaf extract mixture were orally administered 30 minutes before the administration of ethanol and blood was collected at 30 minutes, 1 hour and 2 hours after the administration of ethanol, and the blood alcohol concentration was measured As shown in Fig. 3, the effect of decreasing blood alcohol concentration can be confirmed and compared in 2 hours after administration of ethanol.

As a result of comparing the blood alcohol concentration at 2 hours after ethanol administration, in case of 200 mg / kg of Houttuynia cordata extract and 100 or 200 mg / kg of hot water extract, (MM) blood alcohol concentration was decreased by 1.0 and 0.7 nmol / ul (mM), respectively, compared with the control group treated with ethanol only (4.1 nmol / ul, mM). These results show that 75% and 82.9% of the alcohol concentration in the blood was reduced by 100%, respectively, in the control group treated with ethanol alone.

In the group treated with 400 mg / kg of hydrolyzate of Hovenia duli fruit juice alone, the blood alcohol concentration was decreased to 4.0 and 2.4 nmol / ul (mM), respectively, compared to the ethanol treated group (4.1 nmol / It is possible to confirm that it is. These results show that the alcohol concentration of ethanol was reduced by 2.4% and 41.5%, respectively, when the alcohol concentration of the control group was 100%.

In the experimental group treated with 200 mg / kg and 400 mg / kg of hot water extract of Chazugi leaves, blood alcohol concentration was decreased to 3.2 and 3.1 nmol / ul (mM), respectively, compared with the ethanol treated group (4.1 nmol / It is possible to confirm that it is. These results show that alcohol concentration was reduced by 22% and 24.4%, respectively, when the ethanol concentration of the control group was 100%.

From these results, it was concluded that the effect of reducing the alcohol concentration in the blood was higher when the mixture was treated in the form of a mixture of hot and cold water extracts of Houttuynia fruit and Chazji leaf, .

FIG. 4 is a graph showing a comparison of the blood alcohol concentration with the hangover elimination beverage (YM) being sold on the market as a positive control in the experiment to observe the hangover elimination effect of the mixture of Houttuynia cordata fruit extract and Cha-zhi leaf extract.

As a result of comparing the blood alcohol level at 2 hours after the administration of the positive control sample (YM), 100 mg / kg of the hot water extract of Chamaecyparis obtusa and 200 mg / kg of the hot water extract of Hovenia dulcis In one experimental group, the blood alcohol concentration was 24.3% and 16.8%, respectively, when the blood alcohol concentration of the positive control (YM treated group, 400 mg / kg) was taken as 100%. That is, 75% and 83.2% of the blood alcohol concentration was reduced when the blood alcohol concentration of the positive control group (YM treated group, 400 mg / kg) was taken as 100%, respectively.

In the test group treated with 200 mg of 400 mg / kg of hot-water extract of Hovenia duli fruit, the blood alcohol concentration of 6.5% and 43.5% in the positive control group (YM treated group, 400 mg / kg) The effect of concentration reduction is shown.

In the test group treated with 200 mg / kg and 400 mg / kg of hot water extract of Chazugi leaves, the blood alcohol concentration of the positive control group (YM treated group, 400 mg / kg) was 25% and 28.3% The effect of concentration reduction is shown.

From these results, it was found that the effect of reducing the concentration of alcohol in blood when treated with the mixture of hot - water extract of Hovenia dulcis and hot - water extract of chinchilla leaf was significantly lower than that of the control group, hot - water extract of Hovenia dulcis High.

From this result, it was confirmed that the mixture of hot water extract of Hovenia duli and hot water extract of Chazji leaf was administered at a certain ratio to the body weight of the experimental animals, and it was confirmed that it has an effect of inhibiting hangover caused by alcohol including an increase in the rate of degradation of blood alcohol by natural products. Can be used for the prevention and resolution of hangover caused by alcohol ingestion.

6. Manufacture of functional food for hangover and prevention

Example 1: Functional beverage containing the extract of the present invention

The beverage containing the extract of the present invention contains a mixture of 10.89% by weight of a 1: 1 mixture of a mixture of hot and cold water extract of Hovenia duli and tea leaves extracted according to the method of the present invention, and 0.002% by weight of vitamin B1 0.5 to 10% by weight of oligosaccharide and 0 to 0.1% by weight of citric acid were mixed, and the remaining amount of purified water was added to the mixture. The resulting mixture was stirred at 60 to 65 ° C for 30 minutes to 1 hour And sterilized to prepare a final product. At this time, additives such as sugar, saccharides, and fragrance generally used for imparting flavor can be added according to the taste of the consumer.

The additives may be added, for example, one or more of the following additives, if necessary. Examples of the additive include various kinds of fruit juice such as grapefruit, apple, orange, lemon, pineapple, banana, and pear (may be farmed fruit juice, powdered fruit juice, etc.); Vitamins and provitamins such as palmitoyl retinol, bisbentiamine, riboflavin, pyridoxine hydrochloride, cyanocobalamine, sodium ascorbate, nicotinamide, calcium pantothenate, folic acid, biotin, Water-soluble and fat-soluble vitamins such as cholecalciferol, choline quartet, tocopherol, beta -carotene and the like); Spices (lemon flavor, orange flavor, grapefruit flavor, vanilla essence, etc.); Amino acids, nucleic acids and salts thereof (glutamic acid, sodium glutamate, glycine, alanine, aspartic acid, sodium aspartate, inosine, etc.); Vegetable fibers (polydextrose, pectin, xanthan gum, gum arabic, alginic acid and the like); Minerals or trace elements such as sodium chloride, sodium acetate, magnesium sulfate, potassium chloride, magnesium chloride, magnesium carbonate, calcium chloride, dipotassium phosphate, disodium phosphate, calcium glycerophosphate, sodium ferrous citrate, , Manganese sulfate, copper sulfate, sodium iodide, potassium sorbate, zinc, manganese, copper, iodine, cobalt, etc.).

Example 2: A ring and powder containing the extract of the present invention

The ring containing the extract of the present invention was prepared by mixing 70% by weight of a mixture of the hot-water extract of Hovenia duli and the hot-water extract of Char-grilled leaf, 5% by weight of aloe vera concentrate or aloe alboresense powder, 20% 5 wt% of the kneaded mixture is sterilized, molded through a milling machine and a ring cycle, and the molded mixture is cut through a switcher to cut a predetermined amount. The mixture cut into a certain amount can be obtained by molding with a circulating machine (sugar group), drying the molded ring, sterilizing and cooling the mixture. In addition, the dry sterilized ring can be finely pulverized into micro-units to produce powder.

As a composition for spoiling hangover, the mixture of hot and cold water extracts of Hovenia duli and Chazji leaves is excellent in the hangover relieving action and has excellent hepatotoxic effect without any adverse effect on the human body. Therefore, the health supplement food containing the effective ingredient is easy to take It can be stored for a long time, so it can be useful for the elimination of hangover before and after drinking, and it can be used for protecting the liver. By replacing the hangover elimination raw material with a plant inhabited by nature, it is possible to expect the effect of import substitution and export by industrial production will be.

Claims (6)

Characterized in that the functional food composition for prevention and elimination of hangover, wherein the weight ratio of Hovenous fruit extract and tea leaf extract is 1: 0.5 to 1, is contained in an amount of 0.01 to 95% by weight of the whole composition Functional food for
The functional food for preventing and resolving hangovers according to claim 1, wherein the functional food for hangover prevention and elimination is in the form of powder, granule, tablet, capsule or beverage. delete delete delete delete

Images