WO2014114042A1 - 一种天然橡胶乳汁加工中除臭以及提取蛋白质的方法 - Google Patents

一种天然橡胶乳汁加工中除臭以及提取蛋白质的方法 Download PDF

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WO2014114042A1
WO2014114042A1 PCT/CN2013/076229 CN2013076229W WO2014114042A1 WO 2014114042 A1 WO2014114042 A1 WO 2014114042A1 CN 2013076229 W CN2013076229 W CN 2013076229W WO 2014114042 A1 WO2014114042 A1 WO 2014114042A1
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latex
protein
rubber
weight
container
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PCT/CN2013/076229
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French (fr)
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黄润燕
黄一君
许灿姬
黄一书
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Huang Runyan
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L7/00—Compositions of natural rubber
    • C08L7/02—Latex

Definitions

  • the invention belongs to the technical field of rubber processing, and particularly relates to a technology for preserving and extracting by-products of rubber raw latex.
  • Natural rubber is China's strategic material, its quality is superior to synthetic rubber. It has the quality of synthetic rubber. China has a rubber planting area of 875,000 hectares. The annual output is about 590,000 tons. According to the statistics of the Yunnan Provincial Department of Agriculture, only the rubber planting area in Yunnan province is 7.65 million mu, the output is 7.35 million tons, and the output value is 11.5 billion yuan. It has basically become the economic pillar of agriculture and forestry in Yunnan province. The planting area is large, so various types of rubber processing plants have emerged. Only Yunnan Xishuangbanna natural rubber processing plants have 89, of which more than 30 have an annual output of 10,000 tons. The rubber processing plant is open for a period of eight months each year.
  • the surrounding area of the factory is 2-3 kilometers. It will be stinking. It is no exaggeration to describe dozens of factories at the same time. This kind of scene has caused the local government.
  • the functional departments attach great importance to it. From the rubber-collected rubber processing to the rubber processing plant, the rubber farmer must solidify with formic acid on the day the glue is collected, so that the transportation can be carried out without affecting the quality of the rubber, but the treatment process is lost. Since the anti-corrosion treatment is not carried out after the glue is collected, the protein of the glue undergoes a qualitative change in a short time after being affected by the formic acid, so that every time the rubber processing plant is started, a malodor is generated during the pressing and baking process.
  • Odor gas pollution refers to all gaseous substances that stimulate people's unpleasantness and damage to the living environment. It has been recognized as a typical environmental hazard by many countries in the world. Many developed countries regard it as a kind of single-row public hazard. And special legislation to prevent and control. Foreign efforts to control odor pollution have also been carried out earlier. At present, technologies such as fixed activated carbon adsorption and deodorization have been used in the world. Over the years, China has begun to pay attention to the monitoring and prevention of malodor, and formulated some malodorous compounds.
  • Emission standards (GB14554-93) and supporting analytical methods.
  • One of the targets of the foul-smelling pollution prevention is to meet the emission standards of odorous substances (ammonium trimethylamine, hydrogen sulfide, methyl mercaptan, methyl disulfide, carbon trisulfide and ethylene) as specified in GB 14554-93.
  • the purpose is to eliminate stench and create a odorless and good working and living environment.
  • the methods of international and domestic malodor control mainly include direct combustion method, catalytic nitridation method, ozone deodorization method, activated carbon adsorption method, chemical liquid spray method and biodegradation method.
  • direct combustion method catalytic nitridation method
  • ozone deodorization method activated carbon adsorption method
  • chemical liquid spray method chemical liquid spray method
  • biodegradation method biodegradation method.
  • the 'wood vinegar' addition method is currently used.
  • the treatment of the malodor of the rubber processing plant has high running cost, uncut processing, unstable, and low deodorizing efficiency, and does not eliminate the malodor in the rubber processing process from the essence and the root. In order to eradicate this odor phenomenon in the rubber processing process, it is necessary to start from the source, and it is not waiting for the malodor to be processed during processing.
  • the present invention provides a method for deodorizing the natural rubber milk processing.
  • the technical solution is as follows:
  • a method for deodorizing natural rubber latex processing comprising the following sequential steps:
  • Step 1 Prepare a sodium benzoate solution: Take 10 parts by weight of water into a stainless steel container, control the water temperature between 25-35 ° C, then add 1 part by weight of sodium benzoate while stirring in the same direction in water until the sodium benzoate granules are completely dissolved. , obtaining a sodium benzoate solution;
  • Step 2 Prepare the straw section: Take 20 parts by weight of dry weight straw and cut the straw into 2-3 cm long straw segments;
  • Step 3 Prepare glutinous rice clear liquid: 10 parts by weight of glutinous rice is added to 150 parts by weight of water, after grinding into a slurry, 0.005 parts by weight of ammonium polyacrylate is added to the slurry to carry out a coagulation reaction, the coagulation temperature is controlled to 30 ° C, and the coagulation time is 3-4 h; After the condensation reaction is completed, the supernatant liquid on the surface layer is extracted, and the pH value is 4-5 by adding clear water to obtain a glutinous rice clear liquid;
  • Step 4 Prepare preservative solution: Take the straw section prepared in step 2 into a non-metallic container, take the glutinous rice clear liquid prepared in step 3 into a non-metallic container, soak the straw section for 7-12 hours, and control the temperature at 30-40 ° C to obtain straw. Segment suspension Then, the 80-mesh nylon filter cloth is used to filter the straw section suspension in the non-metallic container, and the obtained filtrate is adjusted to pH 5-6 with water to obtain a preservative solution;
  • Step 5 Preservative treatment: Take 50 parts by weight of the original latex into a plastic container, add the sodium benzoate solution prepared in step 1 at a ratio of 20 ml of sodium benzoate solution per kg of latex at room temperature, and stir in the same direction for 5-7 seconds. ; Then, the preservative solution prepared in the step 4 was added in a ratio of 30 ml of the preservative solution per kg of the latex, stirred for 30 seconds, and allowed to stand for 30 minutes to obtain a preservative-treated latex.
  • the present invention also provides a method of extracting protein and preparing a raw gum using the above-described preservative-treated latex, comprising the following sequential steps:
  • Step 6 taking the anti-corrosion treatment latex into a stainless steel container, under repeated stirring conditions, adding 0.01 parts by weight of diisopropyl fluorophosphoric acid, by adding water to control the pH value of 5-7;
  • Step 7 Extract the protein: An ultraviolet sterilizing lamp is installed in the above-mentioned latex-equipped stainless steel container, and a medium-wave electromagnetic group is installed at the bottom of the stainless steel container, and then the latex is repeatedly stirred, so that the protein in the latex is formed by the ultraviolet rays and electromagnetic repetitive action to form a protein molecule assembly. crystallization; Then, the latex is sent to the ultrasonic extractor through a metering pump to extract the protein;
  • Step 8 Prepare the original rubber: 10 parts by weight of the latex after extracting the protein is placed in a plastic container, and formic acid is added in a ratio of 1 ml of formic acid per kg of latex in the latex, and stirred for 5-7 seconds; then the material of the plastic container is poured into the container. In a container of 10 parts by weight of fresh water, after 2-4 hours, a wet heavy gum which is shaped like a tofu block is obtained.
  • the ultraviolet sterilization lamp in the step 7 has two, is installed in the middle section of the inner wall of the container, and is disposed in a pair diameter, and the ultraviolet light has a wavelength of 0.1-0.4 micrometer;
  • the medium wave electromagnetic group in the step 7 is an electric magnetic disk, and the electromagnetic wave has a wavelength of 50-100 meters and a frequency of 6-30 MHz.
  • the ultrasonic extractor in the step 7 is a pipeline continuous countercurrent ultrasonic extractor;
  • the protein extracted in the step 7 is further concentrated, purified, and vacuum dried to finally obtain a plant protein.
  • the technical scheme of the invention directly adds sodium benzoate to the rubber milk to achieve the purpose of antiseptic, not only does not increase the cost of the rubber farmer, but also eliminates the generation of the odor by the anticorrosion of the latex liquid, and improves the quality of the glue. And without affecting the quality of the rubber raw rubber, the plant protein is extracted, the income of the rubber farmer is increased, the added value of the product is increased, the capital for adding sodium benzoate is compensated, and the production cost is lowered.
  • sodium benzoate can be purchased in the market (quality requirement content is ⁇ 99.5%, water insoluble matter: 0.01%, chloride 0.01%, sulfate 0.005%, iron 0.0005%); Cut into 2-3cm with 20kg dry weight of straw; After adding 10 kg of glutinous rice and adding 150 kg of water to make a slurry, add 5 g of ammonium polyacrylate (mass required to synthesize molecular weight 4 million, polymerization temperature 30 ° C, time 3-4 h) to carry out the condensation on the surface, and prepare with clear water.
  • the pH value of 4-5 is suitable, and the glutinous rice clear liquid is obtained, which is ready for use; Cut the cut straw into a non-metallic container such as ceramic or plastic, soak the straw with glutinous rice liquid for 7-12 hours, control the temperature at 30-40 degrees, and then use 80 mesh nylon filter cloth. Filtration, if the pH value is abnormal, the pH can be adjusted to between 5-6 with water to obtain a 'mixed liquid', which is ready for use.
  • Protein extraction 1. Concentrate the already-preserved latex into a stainless steel container capable of holding 50 kg. Add 10 g of diisopropyl fluorophosphate under constant stirring, but strictly control the balance of pH, pH must be controlled at 5. Between -7, you can add water to adjust; Among them, diisopropylfluorophate is known as diisopropylfluorophate; dissopropylphorofluoridate; molecular formula: C6H14FO3P, (quality requirements sodium formate content, % ⁇ 95.0 - 93.0, NaCl content, % ⁇ 1.00 - 1.50, Na 2 CO 3 content, % ⁇ 3.00 , Na 2 S content, % ⁇ 0.10 - 0.20, moisture content, % ⁇ 1.00 - 1.50); 2, the middle section of the inner wall of the container is installed with two UV sterilization lamps (sold in the market), generally 0.1-0.4 microns, and a wavelength of 50 meters to 100 meters (frequency 6
  • the medium wave electromagnetic group which is a commercially available S3036 type electric disk, enables the latex to reach the protein in the latex under constant stirring, and forms a relative assembly of protein molecules under the action of ultraviolet rays and electromagnetic interference. Then, the latex is sent to the TDCH-7/2200/5400 pipeline type continuous countercurrent ultrasonic extractor produced by Shandong Jining Jinbaite Biological Machinery Co., Ltd. through the metering pump to extract the protein, and the concentrated solution is extracted using DTN-1000 type.
  • the dynamic cycle evaporative concentrator concentrates the protein and purifies it by vacuum drying to obtain 14 kg of plant protein with a protein content of 80%; 3.
  • the latex is divided into 5 parts per 10 kg, placed in a plastic container capable of holding 15 kg, 10 mL of formic acid is added per 10 kg, stirred for 5-7 seconds and then placed in another five.
  • a container containing 10 kg of clean water 35 kg of wet heavy-weight raw rubber like tofu can be formed in 2-4 hours, and it can be sent to a baking rubber processing factory for processing into a rubber product.
  • Protein extraction 1. Concentrate the already-preserved latex into a stainless steel container capable of holding 100 kg. Add 20 g of diisopropyl fluorophosphate under constant stirring, but strictly control the balance of pH. The pH must be controlled at 5. Between -7, you can add water to adjust; 2, the middle section of the inner wall of the container is installed with two UV sterilization lamps (sold in the market), generally 0.1-0.4 microns, and a wavelength of 50 meters to 100 meters (frequency 6 MHz - 30 MHz) installed at the bottom of the container.
  • the medium wave electromagnetic group which is a commercially available S3036 type electric disk, enables the latex to reach the protein in the latex under constant stirring, and forms a relative assembly of protein molecules under the action of ultraviolet rays and electromagnetic interference. Then, the latex is sent to the TDCH-7/2200/5400 pipeline type continuous countercurrent ultrasonic extractor produced by Shandong Jining Jinbaite Biological Machinery Co., Ltd. through the metering pump to extract the protein, and the concentrated solution is extracted using DTN-1000 type.
  • the dynamic circulation evaporative concentrator concentrates the protein, and purifies and vacuums to obtain 29 kg of plant protein with a protein content of 80%; 3.
  • the latex is divided into 10 parts of 10 kg each, placed in 10 plastic containers capable of holding 15 kg, 10 mL of formic acid is added per 10 kg, stirred for 5-7 seconds and then placed in another 10 containers containing 10 kg of clean water can be formed into 75 kg of wet heavy-weight raw rubber like tofu in 2-4 hours, and can be sent to a baking rubber processing factory for processing into a rubber product.
  • Protein extraction 1. Concentrate the already-preserved latex into two stainless steel containers capable of holding 100 kg. Add 20 g of diisopropylfluorophosphoric acid to each stainless steel container under constant stirring, but strictly control the pH. Balance, pH must be controlled between 5-7, can be adjusted by adding water; 2, the middle section of the inner wall of the container is installed with two UV sterilization lamps (sold in the market), generally 0.1-0.4 microns, and a wavelength of 50 meters to 100 meters (frequency 6 MHz - 30 MHz) installed at the bottom of the container.
  • the medium wave electromagnetic group which is a commercially available S3036 type electric disk, enables the latex to reach the protein in the latex under constant stirring, and forms a relative assembly of protein molecules under the action of ultraviolet rays and electromagnetic interference. Then, the latex is sent to the TDCH-7/2200/5400 pipeline type continuous countercurrent ultrasonic extractor produced by Shandong Jining Jinbaite Biological Machinery Co., Ltd. through the metering pump to extract the protein, and the concentrated solution is extracted using DTN-1000 type.
  • the dynamic cycle evaporative concentrator concentrates the protein and purifies it by vacuum drying to obtain 58 kg of plant protein with a protein content of 80%; 3.
  • the latex is divided into 20 parts per 10 kg, placed in a plastic container capable of holding 15 kg, 10 mL of formic acid is added per 10 kg, stirred for 5-7 seconds and then placed in another five.
  • a container containing 10 kg of clean water 140 kg of wet heavy original glue like tofu can be formed in 2-4 hours, and it can be sent to a baking rubber processing factory for processing into a rubber product.
  • Protein is a source of malodorous rubber processing. Moreover, its presence is not only good for the quality of rubber, but also reduces the quality of rubber. It causes serious deterioration of the environment after the quality change in the process of processing, and the quality of the latex after protein extraction is improved.
  • the content of protein is the level of feed quality, usually plant protein, the price is 2.5-30,000 yuan per ton, through the anti-corrosion of the original latex extract protein, improve the quality of latex and other scientific treatment
  • the means to achieve the purpose of curing rubber odor sources, the above examples are all tested in Xishuangbanna Advocate Biotechnology Co., Ltd. in Yunnan province, have achieved good results, and increase the income of rubber farmers to improve the air quality index around the rubber processing plant.

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Abstract

一种天然橡胶乳汁加工中除臭以及提取蛋白质的方法，属于橡胶加工技术领域，解决了现有橡胶收胶后发生质变，在压胶和烤胶时产生恶臭气体，严重污染环境、危害人体健康的问题，本发明首先提供一种天然橡胶乳汁加工中除臭的方法，步骤为：制备安息香酸钠溶液、制备稻草段、制备糯米清液、制备防腐液、防腐处理。本发明还提供一种使用上述胶乳来提取蛋白质和制取原胶的方法：取经防腐处理的胶乳加入二异丙基氟磷酸、提取蛋白质、制取原胶。本发明把安息香酸纳添加到橡胶乳中，消除臭气，提取了植物蛋白，提高了产品的附加值，降低了生产成本。

Description

一种天然橡胶乳汁加工中除臭以及提取蛋白质的方法
技术领域
本发明属于橡胶加工技术领域，特别涉及一种橡胶原胶乳的防腐与提取副产品的技术。
背景技术
天然橡胶是我国的战略物资，品质优于合成橡胶，它具有合成橡胶没有的品质，我国已有橡胶种植面积有87.5万公顷。年产量约59万吨，据云南省农业厅统计，仅云南省橡胶种植面积就有765万亩，产量为735万吨，产值为115亿元，基本成为云南省农、林的经济支柱，橡胶种植面积之大，因此各类大小的橡胶加工厂便应运而生，仅云南省西双版纳州天然橡胶加工厂就有89家，其中年产10000吨的就有30多家。橡胶加工厂每年八个月的开工期工厂周边方圆2－3公里就会恶臭连天，几十家工厂同时开工用'臭不可闻'来形容一点都不为过，这种现场已引起了当地政府职能部门的高度重视。从胶农收胶运到橡胶加工厂这个工程中，胶农必须在收集到胶水的当天用甲酸进行凝固，以便好运输和不影响胶的质量，但这个处理过程有得就有失。由于收胶后没有进行防腐处理，胶水的蛋白质在受到甲酸的影响被氧化后短时间内就发生质变，于是每到收胶加工厂开工，进行压胶和烤胶过程中就产生一种恶臭，一个加工厂周边三公里内时常都闻到橡胶加工时散发出来的恶臭，是真正的'臭不可闻'严重污染环境。恶臭气体污染是指一切刺激臭觉器官引起人们不愉快及损害生活环境的气体物质，它作为一种典型的环境公害已为世界各国所公认，不少发达国家将其作为一种单列公害作为研究，并专项立法实施防治。国外对恶臭污染的治理工作也开展较早，目前世界上采用的，如；固定式活性炭吸附脱臭等技术已有一定历史，连年来，我国开始重视对恶臭的监测与防治，制定了部分恶臭化合物的排放标准（GB14554-93)和配套分析方法。恶臭的污染防治目标之一就是要达到GB14554-93规定的恶臭物质（氨三甲胺、硫化氢、甲硫氢甲硫醇、二甲二硫、三硫化碳和本乙稀等的排放标准，最终目的是要消除恶臭，创造一个无臭的良好的工作、生活环境。
目前，国际国内治理恶臭的手段主要采用：直接燃烧法、催化氮化法、臭氧除臭法、活性炭吸附法、药液喷淋法和生物降解法。在云南西双版纳目前使用'木醋液'添加法。但对橡胶加工厂的恶臭进行处理都存在运行成本高、处理不切底，不稳定，脱臭效率不高，且没有从实质和根源上根除橡胶加工过程中的恶臭。要想根除橡胶加工过程中这种恶臭现象，就得从源头做起，绝不是等在加工时产生了恶臭再去处理。
发明内容
为解决现有橡胶收胶后发生质变，在压胶和烤胶时产生恶臭气体，严重污染环境、危害人体健康的问题，本发明提供一种天然橡胶乳汁加工中除臭的方法 ，其技术方案如下：
一种天然橡胶乳汁加工中除臭的方法，包括以下顺序步骤：
步骤1：制备安息香酸钠溶液：
取10重量份的水放入不锈钢容器中，水温控制在25-35℃之间，然后在水中顺着同一个方向边搅拌边加入1重量份的安息香酸钠，直到安息香酸钠颗粒完全溶解为止，得到安息香酸钠溶液；
步骤2：制备稻草段：
取20重量份干重的稻草，将稻草切成2-3cm长的稻草段；
步骤3：制备糯米清液：
取10重量份的糯米加入150重量份的水中，磨成浆后，在浆中加入0.005重量份的聚 丙 烯酸铵，进行凝聚反应，控制凝聚温度为30℃，凝聚时间为3－4h；
凝聚反应完成后，提取面层上的清液，通过加入清水控制pH值为4-5，得到糯米清液；
步骤4：制备防腐液：
取步骤2制备的稻草段放入非金属容器中，取步骤3制备得到的糯米清液放入非金属容器中，对稻草段浸泡7-12个小时，温度控制在30-40℃，得到稻草段悬浮液；
然后取80目的尼龙滤布对上述非金属容器中的稻草段悬浮液进行过滤，得到的过滤液用清水调整pH值至5-6，得到防腐液；
步骤5：防腐处理：
取50重量份的原胶乳放入塑料容器中，在常温下按照每公斤胶乳加入20毫升安息香酸钠溶液的比例加入步骤1制备得到的安息香酸钠溶液，顺着同一个方向搅拌5-7秒；
然后按照每公斤胶乳加入30毫升防腐液的比例加入步骤4制备得到的防腐液，搅拌30秒，静置 30 分钟后 ，得到经防腐处理的胶乳。
本发明还提供一种 使用上述 经防腐处理的胶乳来提取蛋白质和制取原胶的方法，包括以下顺序步骤：
步骤6：取经防腐处理的胶乳放入不锈钢容器内，在反复的搅拌条件下，加入0.01重量份的二异丙基氟磷酸，通过加入清水控制pH值为5-7；
步骤7：提取蛋白质：
在上述装有胶乳的不锈钢容器中安装紫外线灭菌灯，并在不锈钢容器底部安装中波电磁组，然后反复搅拌胶乳，使胶乳中的蛋白质在紫外线和电磁的反复作用下，形成蛋白分子集结从而结晶；
然后通过计量泵把胶乳送到超声波提取机中，提取得到蛋白质；
步骤8：制取原胶：
将经过提取蛋白质后的10重量份的胶乳放入塑料容器中，在胶乳中按照每公斤胶乳加入1毫升甲酸的比例加入甲酸，搅拌5-7秒钟；然后将塑料容器的物料倒入装有10重量份的清水的容器中，经2-4个小时后，得到结成形似豆腐块状的湿重原胶。
作为本发明方法的优选方案：
所述步骤7中的紫外线灭菌灯有两个，安装在容器的内壁中段，并且呈对径设置，紫外线的波长为0.1-0.4微米；
所述步骤7中的中波电磁组为电磁盘，其电磁波的波长50-100米，频率为6-30兆赫。
作为本发明方法的优选方案：
所述步骤7中 的超声波提取机为管道式连续逆流超声波提取机；
所述步骤7中 提取得到的蛋白质还要经过浓缩、提纯、真空干燥后，最终得到植物蛋白质。
经发明人用了五年时间观察并做了无数次的实验，得知橡胶中所含的30％蛋白质是臭源。收胶后的胶乳在添加甲酸后凝固的同时又对蛋白细胞瞬间氧化破坏致死因而由酸性变臭并产生气体，这种气体极小，不具备冲破已经凝固的胶块的活力，于是就形成了象蛋糕一种的微孔间隙，成为臭气胞，因此在胶厂的压胶和烤胶过程中受到外力和温差的情况下，使这些不饱和的蛋白臭胞爆裂扩散在空气中，因此散发的原臭随风漂移，慢慢形成饱和，到了一定的程度后形成饱和后与空气的比重后形成臭雾降落后才能自然地消除这种恶臭。
在食品中臭气的产生，最常见的是蛋白和脂肪细胞的败变坏死后变形成惰性臭气体，橡胶乳液含有30％的蛋白质，要防止这些活跃的蛋白质在较长的时间内不产生质变，那就必须寻找一种易穿透细胞膜进入细胞体内，并能干扰蛋白细胞膜的通透性，抑制细胞膜对氨基酸的吸收，进入蛋白细胞体内电离酸从细胞内得碱储，抑制细胞的呼吸酶气的活性，阻止乙酰辅酶A缩合反应，防止质变发酵起到了防腐的目的的防腐剂，因此，安息香酸钠是目前最理想的替代品。
本发明技术方案直接把安息香酸纳添加到橡胶乳中，达到防腐的目的，不但不增加胶农的成本支出，而且通过对乳胶液的防腐从根本上消除臭气的产生的同时提高胶的质量，并在不影响橡胶原胶质量的前提下提取了植物蛋白，增加胶农的收入，提高了产品的附加值，弥补因添加安息香酸钠的资金，降低了生产成本。
具体实施方式
按以下方式进行备料 ：
用10公斤的清水放在不锈钢的容器中，水温度控制在25-35度之间，然后徐徐地加入1公斤的安息香酸钠边加入边搅拌，顺着一个方向搅拌，看不到安息香酸钠颗粒并完全溶解为止，静置待用。其中，安息香酸钠可在市面上购买 (质量要求含量为≥99.5％，水不溶物：0.01％、氯化物0.01％、硫酸盐0.005％、铁0.0005％)；
用20公斤干重的稻草切成2-3cm；
用10公斤的糯米加入150公斤的水磨成浆后，加入5g的聚丙烯酸铵（质量要求合成分子量400万，聚合温度30℃，时间3－4h)进行凝聚后取面上的清夜，用清水调制pH值4-5为宜，得出糯米清液，待用；
将切好的稻草，盛在一个陶瓷或塑料等非金属的容器装入内，用糯米清液对稻草进行浸泡7-12个小时，温度控制在30-40度，然后用80目的尼龙滤布进行过滤，如pH值出现异常时可用清水将pH值调整至在5-6之间，得到'混合液'，待用。
实施例1 ：
用50公斤的原胶乳放在能承装50公斤的塑料容器中，并在常温下加入已经调好备用的安息香酸钠1000mL，进行顺时针方向搅拌5-7秒后再加入1500mL的混合液体搅拌30秒，静置 30 分钟 ，这时的胶乳就完全得到了全方面的防腐，即使放置10-15天也不会出现蛋白质质变发臭现象。
蛋白质提取：
1 、将已经防腐后的胶乳集中在能盛装50公斤的不锈钢容器内，在不停的搅拌情况下，加入10g二异丙基氟磷酸，但要严格控制酸碱度的平衡，pH值必须控制在5-7之间，可以加入清水进行调节；
其中，二异丙基氟磷酸的英文名为diisopropylfluorophate；dissopropylphorofluoridate；分子式：C6H14FO3P, ( 质量要求 甲酸钠含量，% ≥ 95.0 - 93.0，NaCl含量，% ≤ 1.00 -1.50，Na2CO3含量，% ≤ 3.00，Na2S含量，% ≤ 0.10 - 0.20，水份含量，% ≤ 1.00 -1.50 ) ；
2 、容器的内壁中段呈对径设置分别安装两个紫外线灭菌灯（市场有卖），一般为 0.1-0.4 微米，并在容器底部安装一个波长50米至100米（频率6兆赫-30兆赫）的中波电磁组，所述中波电磁组为市售的S3036型 电磁盘 ，使乳胶在不停的搅拌中达到乳胶中的蛋白质在紫外线作用和电磁的反复干扰下，形成蛋白分子相对集结，然后通过计量泵把乳胶送到由山东济宁金百特生物机械有限公司生产的TDCH-7／2200／5400型管道式连续逆流超声波提取机进行提取蛋白质，提取后的浓缩使用DTN-1000型单效动态循环蒸发浓缩器对蛋白质浓缩，提纯真空干燥就可以得到蛋白质含量80%的14公斤的植物蛋白质;
3、经提取蛋白质后的乳胶依次分成每份10公斤共5份，放在能承装15公斤的塑料容器中，每10公斤加入10mL甲酸，进行搅拌5-7秒钟后放在另五个装有10公斤清水的容器中，2-4个小时就可以结成形似豆腐块状的湿重原胶35公斤，就可以送到烤胶加工厂进行加工成橡胶成品。
实施例2 ：
用100公斤的原胶乳放在能承装100公斤的塑料容器中，并在常温下加入已经调好备用的安息香酸钠2000mL，进行顺时针方向搅拌5-7秒后再加入3000mL的混合液体搅拌30秒，静置30分钟，这时的胶乳就完全得到了全方面的防腐，即使放置10-15天也不会出现蛋白质质变发臭现象。
蛋白质提取：
1 、将已经防腐后的胶乳集中在能盛装100公斤的不锈钢容器内，在不停的搅拌情况下，加入20g二异丙基氟磷酸，但要严格控制酸碱度的平衡，PH值必须控制在5-7之间，可以加入清水进行调节；
2 、容器的内壁中段呈对径设置分别安装两个紫外线灭菌灯（市场有卖），一般为 0.1-0.4 微米，并在容器底部安装一个波长50米至100米（频率6兆赫-30兆赫）的中波电磁组，所述中波电磁组为市售的S3036型 电磁盘 ，使乳胶在不停的搅拌中达到乳胶中的蛋白质在紫外线作用和电磁的反复干扰下，形成蛋白分子相对集结，然后通过计量泵把乳胶送到由山东济宁金百特生物机械有限公司生产的TDCH-7／2200／5400型管道式连续逆流超声波提取机进行提取蛋白质，提取后的浓缩使用DTN-1000型单效动态循环蒸发浓缩器对蛋白质浓缩，提纯真空干燥就可以得到蛋白质含量80%的29公斤的植物蛋白质;
3、经提取蛋白质后的乳胶依次分成每份10公斤共10份，放在10个能承装15公斤的塑料容器中，每10公斤加入10mL甲酸，进行搅拌5-7秒钟后放在另10个装有10公斤清水的容器中，2-4个小时就可以结成形似豆腐块状的湿重原胶75公斤，就可以送到烤胶加工厂进行加工成橡胶成品。
实施例3 ：
用200公斤的原胶乳放在2个能承装100公斤的塑料容器中，并在常温下每100公斤胶乳加入已经调好备用的安息香酸钠2000mL，进行顺时针方向搅拌5-7秒后每100公斤胶乳再加入3000mL的混合液体搅拌30秒，静置30分钟，这时的胶乳就完全得到了全方面的防腐，即使放置10-15天也不会出现蛋白质质变发臭现象。
蛋白质提取：
1 、将已经防腐后的胶乳集中在2个能盛装100公斤的不锈钢容器内，在不停的搅拌情况下，在每个不锈钢容器内加入20g二异丙基氟磷酸，但要严格控制酸碱度的平衡，PH值必须控制在5-7之间，可以加入清水进行调节；
2 、容器的内壁中段呈对径设置分别安装两个紫外线灭菌灯（市场有卖），一般为 0.1-0.4 微米，并在容器底部安装一个波长50米至100米（频率6兆赫-30兆赫）的中波电磁组，所述中波电磁组为市售的S3036型 电磁盘 ，使乳胶在不停的搅拌中达到乳胶中的蛋白质在紫外线作用和电磁的反复干扰下，形成蛋白分子相对集结，然后通过计量泵把乳胶送到由山东济宁金百特生物机械有限公司生产的TDCH-7／2200／5400型管道式连续逆流超声波提取机进行提取蛋白质，提取后的浓缩使用DTN-1000型单效动态循环蒸发浓缩器对蛋白质浓缩，提纯真空干燥就可以得到蛋白质含量80%的58公斤的植物蛋白质;
3、经提取蛋白质后的乳胶依次分成每份10公斤共20份，放在能承装15公斤的塑料容器中，每10公斤加入10mL甲酸，，进行搅拌5-7秒钟后放在另五个装有10公斤清水的容器中，2-4个小时就可以结成形似豆腐块状的湿重原胶140公斤，就可以送到烤胶加工厂进行加工成橡胶成品。
蛋白质是橡胶加工的恶臭源，况且它的存在不但对橡胶的质量没有好处，而且降低了橡胶的品质，在加工的过程中产生质变后严重污染环境，提取蛋白质后的胶乳品质有所提高外，从根本上消除橡胶加工的恶臭现象，蛋白质的含量多少是饲料质量的高低，通常植物蛋白，售价每吨2.5-3万元，通过对原胶乳的防腐提取蛋白，提高胶乳的质量等科学处理手段达到根治橡胶臭源的目的，以上的实例均在云南省西双版纳倡华生物科技有限公司中试，均取得良好的效果，和增加胶农收入提高橡胶加工厂周边空气质量指数。

Claims (5)

  1. 一种天然橡胶乳汁加工中除臭的方法，其特征在于，包括以下顺序步骤：
    步骤1：制备安息香酸钠溶液：
    取10重量份的水放入不锈钢容器中，水温控制在25-35℃之间，然后在水中顺着同一个方向边搅拌边加入1重量份的安息香酸钠，直到安息香酸钠颗粒完全溶解为止，得到安息香酸钠溶液；
    步骤2：制备稻草段：
    取20重量份干重的稻草，将稻草切成2-3cm长的稻草段；
    步骤3：制备糯米清液：
    取10重量份的糯米加入150重量份的水中，磨成浆后，在浆中加入0.005重量份的聚 丙 烯酸铵，进行凝聚反应，控制凝聚温度为30℃，凝聚时间为3－4h；
    凝聚反应完成后，提取面层上的清液，通过加入清水控制pH值为4-5，得到糯米清液；
    步骤4：制备防腐液：
    取步骤2制备的稻草段放入非金属容器中，取步骤3制备得到的糯米清液放入非金属容器中，对稻草段浸泡7-12个小时，温度控制在30-40℃，得到稻草段悬浮液；
    然后取80目的尼龙滤布对上述非金属容器中的稻草段悬浮液进行过滤，得到的过滤液用清水调整pH值至5-6，得到防腐液；
    步骤5：防腐处理：
    取50重量份的原胶乳放入塑料容器中，在常温下按照每公斤胶乳加入20毫升安息香酸钠溶液的比例加入步骤1制备得到的安息香酸钠溶液，顺着同一个方向搅拌5-7秒；
    然后按照每公斤胶乳加入30毫升防腐液的比例加入步骤4制备得到的防腐液，搅拌30秒，静置 30 分钟后 ，得到经防腐处理的胶乳。
  2. 使用权利要求1所述的 经防腐处理的胶乳来提取蛋白质和制取原胶的方法，其特征在于，包括以下顺序步骤：
    步骤6：取经防腐处理的胶乳放入不锈钢容器内，在反复的搅拌条件下，加入0.01重量份的二异丙基氟磷酸，通过加入清水控制pH值为5-7；
    步骤7：提取蛋白质：
    在上述装有胶乳的不锈钢容器中安装紫外线灭菌灯，并在不锈钢容器底部安装中波电磁组，然后反复搅拌胶乳，使胶乳中的蛋白质在紫外线和电磁的反复作用下，形成蛋白分子集结从而结晶；
    然后通过计量泵把胶乳送到超声波提取机中，提取得到蛋白质；
    步骤8：制取原胶：
    将经过提取蛋白质后的10重量份的胶乳放入塑料容器中，在胶乳中按照每公斤胶乳加入1毫升甲酸的比例加入甲酸，搅拌5-7秒钟；然后将塑料容器的物料倒入装有10重量份的清水的容器中，经2-4个小时后，得到结成形似豆腐块状的湿重原胶。
  3. 根据权利要求2所述的提取蛋白质和制取原胶的方法，其特征在于：
    所述步骤7中的紫外线灭菌灯有两个，安装在容器的内壁中段，并且呈对径设置，紫外线的波长为0.1-0.4微米；
    所述步骤7中的中波电磁组为电磁盘，其电磁波的波长50-100米，频率为6-30兆赫。
  4. 根据权利要求3所述的提取蛋白质和制取原胶的方法，其特征在于：
    所述步骤7中的超声波提取机为管道式连续逆流超声波提取机。
  5. 根据权利要求3所述的提取蛋白质和制取原胶的方法，其特征在于：
    所述步骤7中提取得到的蛋白质还要经过浓缩、提纯、真空干燥后，最终得到植物蛋白质。
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